GR407658

EST Overview


Name	GR407658
Unique Name	GR407658
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	219

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR407658 vs. SwissProt
Match: IN21B_ORYSJ (Protein IN2-1 homolog B OS=Oryza sativa subsp. japonica GN=GSTZ5 PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 2.400e-17
Identity = 41/74 (55.41%), Postives = 55/74 (74.32%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSL--KGDAVQQ 218
            VYPENKVPSLEHN +V GESLDL+KY+D NFEG  LLP+D  ++++ E+LL++ D F K  + S+  KGD   +
Sbjct:   79 VYPENKVPSLEHNNQVKGESLDLVKYIDTNFEGPALLPDDSEKQQFAEELLAYTDAFNKASYSSIVAKGDVCDE 152

[Close]

BLAST of GR407658 vs. SwissProt
Match: IN21B_ORYSI (Protein IN2-1 homolog B OS=Oryza sativa subsp. indica GN=GSTZ5 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 2.400e-17
Identity = 41/74 (55.41%), Postives = 55/74 (74.32%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSL--KGDAVQQ 218
            VYPENKVPSLEHN +V GESLDL+KY+D NFEG  LLP+D  ++++ E+LL++ D F K  + S+  KGD   +
Sbjct:   79 VYPENKVPSLEHNNQVKGESLDLVKYIDTNFEGPALLPDDSEKQQFAEELLAYTDAFNKASYSSIVAKGDVCDE 152

[Close]

BLAST of GR407658 vs. SwissProt
Match: IN21A_ORYSJ (Protein IN2-1 homolog A OS=Oryza sativa subsp. japonica GN=Os03g0283200 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.115e-14
Identity = 33/65 (50.77%), Postives = 50/65 (76.92%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSL 197
            VY +  VPSLEHNGK++GESLDLIKY+D++FEG  LLP D  ++++ ++L+++ + F K L+  L
Sbjct:   78 VYEQGTVPSLEHNGKIMGESLDLIKYIDSHFEGPALLPEDPEKRQFADELIAYANAFTKALYSPL 142

[Close]

BLAST of GR407658 vs. SwissProt
Match: IN21_MAIZE (Protein IN2-1 OS=Zea mays GN=IN2-1 PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 7.991e-13
Identity = 32/69 (46.38%), Postives = 48/69 (69.57%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGDA 209
            VY +  VPSLEH+ +V GESLDLI+Y+D+NF+G  LLP D A++++ ++L +  + F K L+  L   A
Sbjct:   78 VYAQGTVPSLEHDSEVRGESLDLIRYIDSNFDGPALLPEDAAKRQFADELFASANAFTKALYSPLLSHA 146

[Close]

BLAST of GR407658 vs. TrEMBL
Match: Q9FQ95_SOYBN (In2-1 protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 4.687e-30
Identity = 64/72 (88.89%), Postives = 69/72 (95.83%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGDAVQQ 218
            VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPL P+D A+KE+GE+L+SHVDTF KDLFVSLKGDAVQQ
Sbjct:   73 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLFPSDPAKKEFGEQLISHVDTFSKDLFVSLKGDAVQQ 144

[Close]

BLAST of GR407658 vs. TrEMBL
Match: C6TBE3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 4.687e-30
Identity = 64/72 (88.89%), Postives = 69/72 (95.83%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGDAVQQ 218
            VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPL P+D A+KE+GE+L+SHVDTF KDLFVSLKGDAVQQ
Sbjct:   73 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLFPSDPAKKEFGEQLISHVDTFSKDLFVSLKGDAVQQ 144

[Close]

BLAST of GR407658 vs. TrEMBL
Match: Q76KW1_PEA (Glutathione S-transferase OS=Pisum sativum GN=GST PE=2 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.276e-27
Identity = 59/72 (81.94%), Postives = 66/72 (91.67%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGDAVQQ 218
            VYPENKVPSLEHNGKVLGESLDL+KYVDANFEG PL PND A+KE+ E+LLSH+DTF+KDL+ SLKGD VQQ
Sbjct:   70 VYPENKVPSLEHNGKVLGESLDLVKYVDANFEGAPLSPNDPAKKEFAEQLLSHLDTFLKDLYGSLKGDTVQQ 141

[Close]

BLAST of GR407658 vs. TrEMBL
Match: B7FHT3_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 1.843e-26
Identity = 58/72 (80.56%), Postives = 64/72 (88.89%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGDAVQQ 218
            VY ENKVPSLEHNGKVLGESLDLIKY+DANF+G PL PND A+KE+ E+LLSHVDTF K+L VSLKGD VQQ
Sbjct:   73 VYLENKVPSLEHNGKVLGESLDLIKYIDANFDGPPLFPNDPAKKEFAEQLLSHVDTFTKELLVSLKGDTVQQ 144

[Close]

BLAST of GR407658 vs. TrEMBL
Match: B7FIE5_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 4.540e-25
Identity = 53/72 (73.61%), Postives = 64/72 (88.89%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGDAVQQ 218
            VYPENKVPSLEHNGKVLGESLDLI Y+D NFEG  L+P+D A+KE+G++L+SHVDTF KDL+ SLKGD ++Q
Sbjct:   72 VYPENKVPSLEHNGKVLGESLDLISYIDVNFEGLSLVPSDPAKKEFGDQLISHVDTFTKDLYSSLKGDPIKQ 143

[Close]

BLAST of GR407658 vs. TrEMBL
Match: C6TMJ4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 7.248e-23
Identity = 48/72 (66.67%), Postives = 60/72 (83.33%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGDAVQQ 218
            VYP NKVPSLEHN KVLGESLDL++Y+DANFEG PL P D A++E+GE+L+SHVDTF   ++ + KGD +QQ
Sbjct:   73 VYPTNKVPSLEHNSKVLGESLDLMRYIDANFEGAPLFPTDPAKREFGEQLISHVDTFTSGIYPTFKGDPIQQ 144

[Close]

BLAST of GR407658 vs. TrEMBL
Match: D6BR66_9ROSI (Glutathione S-transferase omega OS=Jatropha curcas PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.109e-22
Identity = 49/71 (69.01%), Postives = 60/71 (84.51%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGDAVQ 215
            VYP NKVP+LEHNGK++GESLDLIKY+DANFEG  LLP+D A+KE+ E+L S+ DTF K +F S KGDAV+
Sbjct:   77 VYPANKVPALEHNGKIIGESLDLIKYIDANFEGPSLLPDDPAKKEFAEELFSYTDTFTKTVFTSFKGDAVK 147

[Close]

BLAST of GR407658 vs. TrEMBL
Match: Q9LZ06_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana GN=F9G14_100 PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.784e-21
Identity = 46/68 (67.65%), Postives = 58/68 (85.29%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGD 206
            VYPENKVP+LEHNGK++GESLDLIKY+D  FEG  L P D A++E+G++LL + DTFVK ++VSLKGD
Sbjct:   74 VYPENKVPALEHNGKIIGESLDLIKYLDNTFEGPSLYPEDHAKREFGDELLKYTDTFVKTMYVSLKGD 141

[Close]

BLAST of GR407658 vs. TrEMBL
Match: Q8LF01_ARATH (In2-1 protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.784e-21
Identity = 46/68 (67.65%), Postives = 58/68 (85.29%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGD 206
            VYPENKVP+LEHNGK++GESLDLIKY+D  FEG  L P D A++E+G++LL + DTFVK ++VSLKGD
Sbjct:   74 VYPENKVPALEHNGKIIGESLDLIKYLDNTFEGPSLYPEDHAKREFGDELLKYTDTFVKTMYVSLKGD 141

[Close]

BLAST of GR407658 vs. TrEMBL
Match: D2WL72_POPTR (Lambda class glutathione transferase GSTL2 OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.182e-19
Identity = 44/72 (61.11%), Postives = 57/72 (79.17%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGDAVQQ 218
            VYP NKVPSLEHNGK+ GESLDLIKY+++NF+G  LLP D A+KE+ E+L S+ DTF + +F S KGD  ++
Sbjct:   49 VYPPNKVPSLEHNGKITGESLDLIKYLESNFQGPSLLPEDPAKKEFAEELFSYTDTFNRTVFTSFKGDPAKE 120

[Close]

BLAST of GR407658 vs. TAIR peptide
Match: AT5G02790.1 (| Symbols: GSTL3 | Glutathione S-transferase family protein | chr5:632877-634858 FORWARD LENGTH=235)

HSP 1 Score: 103.605 bits (257), Expect = 2.608e-23
Identity = 46/68 (67.65%), Postives = 58/68 (85.29%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKGD 206
            VYPENKVP+LEHNGK++GESLDLIKY+D  FEG  L P D A++E+G++LL + DTFVK ++VSLKGD
Sbjct:   74 VYPENKVPALEHNGKIIGESLDLIKYLDNTFEGPSLYPEDHAKREFGDELLKYTDTFVKTMYVSLKGD 141

[Close]

BLAST of GR407658 vs. TAIR peptide
Match: AT5G02780.2 (| Symbols: GSTL1 | glutathione transferase lambda 1 | chr5:630957-632485 FORWARD LENGTH=235)

HSP 1 Score: 97.4413 bits (241), Expect = 1.869e-21
Identity = 47/73 (64.38%), Postives = 59/73 (80.82%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVD-TFVKDLFVSLKGDAVQQ 218
            V P NKVP+LEHNGK+ GESLDLIKYVD+NF+G  L P D A++E+GE+LL +VD TFVK +F S KGD V++
Sbjct:   76 VNPANKVPALEHNGKITGESLDLIKYVDSNFDGPSLYPEDSAKREFGEELLKYVDETFVKTVFGSFKGDPVKE 148

[Close]

BLAST of GR407658 vs. TAIR peptide
Match: AT5G02780.1 (| Symbols: GSTL1 | glutathione transferase lambda 1 | chr5:630957-632485 FORWARD LENGTH=237)

HSP 1 Score: 97.4413 bits (241), Expect = 1.869e-21
Identity = 47/73 (64.38%), Postives = 59/73 (80.82%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVD-TFVKDLFVSLKGDAVQQ 218
            V P NKVP+LEHNGK+ GESLDLIKYVD+NF+G  L P D A++E+GE+LL +VD TFVK +F S KGD V++
Sbjct:   76 VNPANKVPALEHNGKITGESLDLIKYVDSNFDGPSLYPEDSAKREFGEELLKYVDETFVKTVFGSFKGDPVKE 148

[Close]

BLAST of GR407658 vs. TAIR peptide
Match: AT3G55040.1 (| Symbols: GSTL2 | glutathione transferase lambda 2 | chr3:20398718-20400305 REVERSE LENGTH=292)

HSP 1 Score: 78.1814 bits (191), Expect = 1.173e-15
Identity = 37/67 (55.22%), Postives = 49/67 (73.13%), Query Frame = 3

Query:    3 VYPENKVPSLEHNGKVLGESLDLIKYVDANFEGTPLLPNDLARKEYGEKLLSHVDTFVKDLFVSLKG 203
            VY  NKVP+LEHN +VLGESLDLIKY+D NFEG  L P+ L ++   ++LLS+ D+F K +  +L G
Sbjct:  126 VYSANKVPALEHNNRVLGESLDLIKYIDTNFEGPSLTPDGLEKQVVADELLSYTDSFSKAVRSTLNG 192

The following BLAST results are available for this feature:

BLAST of GR407658 vs. SwissProt
BLAST of GR407658 vs. TrEMBL
BLAST of GR407658 vs. TAIR peptide

BLAST of GR407658 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
IN21B_ORYSJ	2.400e-17	55.41	Protein IN2-1 homolog B OS=Oryza sativa subsp. jap...	[more]
IN21B_ORYSI	2.400e-17	55.41	Protein IN2-1 homolog B OS=Oryza sativa subsp. ind...	[more]
IN21A_ORYSJ	1.115e-14	50.77	Protein IN2-1 homolog A OS=Oryza sativa subsp. jap...	[more]
IN21_MAIZE	7.991e-13	46.38	Protein IN2-1 OS=Zea mays GN=IN2-1 PE=2 SV=1	[more]

BLAST of GR407658 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9FQ95_SOYBN	4.687e-30	88.89	In2-1 protein OS=Glycine max PE=2 SV=1	[more]
C6TBE3_SOYBN	4.687e-30	88.89	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q76KW1_PEA	1.276e-27	81.94	Glutathione S-transferase OS=Pisum sativum GN=GST ...	[more]
B7FHT3_MEDTR	1.843e-26	80.56	Putative uncharacterized protein OS=Medicago trunc...	[more]
B7FIE5_MEDTR	4.540e-25	73.61	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6TMJ4_SOYBN	7.248e-23	66.67	Putative uncharacterized protein OS=Glycine max PE...	[more]
D6BR66_9ROSI	2.109e-22	69.01	Glutathione S-transferase omega OS=Jatropha curcas...	[more]
Q9LZ06_ARATH	6.784e-21	67.65	Putative uncharacterized protein OS=Arabidopsis th...	[more]
Q8LF01_ARATH	6.784e-21	67.65	In2-1 protein OS=Arabidopsis thaliana PE=2 SV=1	[more]
D2WL72_POPTR	2.182e-19	61.11	Lambda class glutathione transferase GSTL2 OS=Popu...	[more]

BLAST of GR407658 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G02790.1	2.608e-23	67.65	\| Symbols: GSTL3 \| Glutathione S-transferase famil...	[more]
AT5G02780.2	1.869e-21	64.38	\| Symbols: GSTL1 \| glutathione transferase lambda ...	[more]
AT5G02780.1	1.869e-21	64.38	\| Symbols: GSTL1 \| glutathione transferase lambda ...	[more]
AT3G55040.1	1.173e-15	55.22	\| Symbols: GSTL2 \| glutathione transferase lambda ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012335	Thioredoxin fold	GENE3D	3.40.30.10	no description	coord: 2..56 score: 8.8
IPR012336	Thioredoxin-like fold	SUPERFAMILY	52833	Thioredoxin-like	coord: 1..39 score: 1.6
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 2..56 score: 1.3
None	No IPR available	PANTHER	PTHR11260:SF14	GLUTATHIONE-S-TRANSFERASE OMEGA	coord: 2..56 score: 1.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR407658 ID=GR407658; Name=GR407658; organism=Cicer arietinum; type=EST; length=219bp
AAGTATATCCTGAAAATAAGGTGCCATCTTTGGAGCACAATGGCAAGGTA
TTGGGGGAAAGTCTTGATTTGATCAAATATGTTGATGCTAACTTTGAAGG
GACTCCTCTTCTTCCCAATGATCTGGCCAGGAAGGAGTACGGTGAGAAGT
TGTTATCCCATGTTGATACATTCGTCAAAGACTTGTTTGTTTCGTTAAAA
GGGGATGCTGTGCAACAAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR012335	Thioredoxin fold
IPR012336	Thioredoxin-like_fold

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