GR420864

EST Overview


Name	GR420864
Unique Name	GR420864
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	466

Libraries


Library Name	Type
LIBEST_024785 ICCV2 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28

Homology

[Close]

BLAST of GR420864 vs. SwissProt
Match: PER15_IPOBA (Peroxidase 15 OS=Ipomoea batatas GN=pod PE=1 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 5.601e-9
Identity = 36/80 (45.00%), Postives = 47/80 (58.75%), Query Frame = -1

Query:  242 NDKYYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESF-----KAGSIGVITGNKGEIRKHCNFVN 466
            ++ Y+SNL+  +       ELFSTSGA TI+IVN FS +Q AF     FESF       G+I  +TG+ GEIR +C   N
Sbjct:  253 DNNYFSNLQTNRGLLQSDQELFSTSGAPTIAIVNNFSANQTAF-----FESFVQSMINMGNISPLTGSNGEIRSNCRRPN 327

[Close]

BLAST of GR420864 vs. SwissProt
Match: PER22_ARATH (Peroxidase 22 OS=Arabidopsis thaliana GN=PER22 PE=1 SV=2)

HSP 1 Score: 57.7658 bits (138), Expect = 2.780e-8
Identity = 37/95 (38.95%), Postives = 55/95 (57.89%), Query Frame = -1

Query:  203 NDKYYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESF-----KAGSIGVITGNKGEIRKHCNFVNK--KSVEQDIVSVAS 466
            + +YY+NL+  K       ELFST GADTI +VN++S+D   F     F +F     + G++  +TG +GEIR++C  VN   + VE D   V+S
Sbjct:  259 DSQYYTNLRNGKGLIQSDQELFSTPGADTIPLVNQYSSDMSVF-----FRAFIDAMIRMGNLRPLTGTQGEIRQNCRVVNPRIRVVENDDGVVSS 348

[Close]

BLAST of GR420864 vs. SwissProt
Match: PER3_ARMRU (Peroxidase C3 OS=Armoracia rusticana GN=PRXC3 PE=3 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 4.014e-7
Identity = 30/75 (40.00%), Postives = 45/75 (60.00%), Query Frame = -1

Query:  236 KYYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESFKAGSIGVITGNKGEIRKHCNFVNKK 460
            +YY+NL+  K       ELFST GADTI +VN +S++  AF         + G++  +TG +GEIR++C  VN +
Sbjct:  261 QYYTNLRNGKGLIQSDQELFSTPGADTIPLVNLYSSNTFAFFGAFVDAMIRMGNLRPLTGTQGEIRQNCRVVNSR 335

[Close]

BLAST of GR420864 vs. SwissProt
Match: PER54_ARATH (Peroxidase 54 OS=Arabidopsis thaliana GN=PER54 PE=2 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 5.242e-7
Identity = 31/83 (37.35%), Postives = 48/83 (57.83%), Query Frame = -1

Query:  233 NDKYYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESF-----KAGSIGVITGNKGEIRKHCNFVNKKS 466
            ++ Y++NL+          ELFS +G+ T+ IVN F+++Q  F     FE+F     K G+I  +TG+ GEIR+ C  VN +S
Sbjct:  260 DNNYFTNLQSNNGLLQSDQELFSNTGSATVPIVNSFASNQTLF-----FEAFVQSMIKMGNISPLTGSSGEIRQDCKVVNGQS 337

[Close]

BLAST of GR420864 vs. TrEMBL
Match: Q18PQ8_PEA (Peroxidase OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.039e-21
Identity = 60/91 (65.93%), Postives = 67/91 (73.63%), Query Frame = -1

Query:  200 YYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESFKA-----GSIGVITGNKGEIRKHCNFVNKKSVEQDIVSVAST 457
            YYSNL+VKK       ELFSTSGADTISIVNKFS DQ+AF     FESFKA     G+IGV+TG +GEIRK CNFVN KS E  ++SVAST
Sbjct:  257 YYSNLQVKKGLLQSDQELFSTSGADTISIVNKFSADQNAF-----FESFKAAMIKMGNIGVLTGKQGEIRKQCNFVNSKSAELGLISVAST 342

[Close]

BLAST of GR420864 vs. TrEMBL
Match: A4UN78_MEDTR (Peroxidase OS=Medicago truncatula GN=PRX3 PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.357e-21
Identity = 60/91 (65.93%), Postives = 68/91 (74.73%), Query Frame = -1

Query:  200 YYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESFKA-----GSIGVITGNKGEIRKHCNFVNKKSVEQDIVSVAST 457
            YYSNL+VKK       ELFSTSG+DTISIVNKF+TDQ AF     FESFKA     G+IGV+TG +GEIRK CNFVN KSVE  +V+VAST
Sbjct:  260 YYSNLQVKKGLLQSDQELFSTSGSDTISIVNKFATDQKAF-----FESFKAAMIKMGNIGVLTGKQGEIRKQCNFVNSKSVELGLVNVAST 345

[Close]

BLAST of GR420864 vs. TrEMBL
Match: C6TB83_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 3.949e-21
Identity = 57/90 (63.33%), Postives = 68/90 (75.56%), Query Frame = -1

Query:  203 YYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESFKA-----GSIGVITGNKGEIRKHCNFVNKKSVEQDIVSVAS 457
            Y+SNL+VKK       ELFST GADTI IVN+FS+DQ+ F     F++F+A     G+IGV+TGNKGEIRKHCNFVNKKSVE DI +VAS
Sbjct:  258 YFSNLQVKKGLLQSDQELFSTPGADTIPIVNRFSSDQNVF-----FDAFEASMIKMGNIGVLTGNKGEIRKHCNFVNKKSVELDIATVAS 342

[Close]

BLAST of GR420864 vs. TrEMBL
Match: Q40365_MEDSA (Peroxidase OS=Medicago sativa GN=pxdA PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.158e-21
Identity = 58/90 (64.44%), Postives = 66/90 (73.33%), Query Frame = -1

Query:  203 YYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESFKA-----GSIGVITGNKGEIRKHCNFVNKKSVEQDIVSVAS 457
            YYSNL+VKK       ELFSTSGADTISIV+KFSTDQ+AF     FESFKA     G+IGV+TG KGEIRK CNFVN  S E D+ ++AS
Sbjct:  250 YYSNLQVKKGLLQSDQELFSTSGADTISIVDKFSTDQNAF-----FESFKAAMIKMGNIGVLTGTKGEIRKQCNFVNSNSAELDLATIAS 334

[Close]

BLAST of GR420864 vs. TrEMBL
Match: Q43790_MEDSA (Peroxidase1B OS=Medicago sativa GN=prx1B PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 8.798e-21
Identity = 57/90 (63.33%), Postives = 67/90 (74.44%), Query Frame = -1

Query:  203 YYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESFKA-----GSIGVITGNKGEIRKHCNFVNKKSVEQDIVSVAS 457
            YYSNL+VKK       ELFSTSG+DTISIVNKF+TDQ AF     FESF+A     G+IGV+TGN+GEIRK CNFVN KS E  +++VAS
Sbjct:  259 YYSNLQVKKGLLQSDQELFSTSGSDTISIVNKFATDQKAF-----FESFRAAMIKMGNIGVLTGNQGEIRKQCNFVNSKSAELGLINVAS 343

[Close]

BLAST of GR420864 vs. TrEMBL
Match: O23961_SOYBN (Peroxidase OS=Glycine max GN=GMIPER1 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.501e-20
Identity = 57/90 (63.33%), Postives = 66/90 (73.33%), Query Frame = -1

Query:  203 YYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESFKA-----GSIGVITGNKGEIRKHCNFVNKKSVEQDIVSVAS 457
            Y+SNL+VKK       ELFST GADTI IVN+FS+DQ  F     F++F+A     G+IGV+TG KGEIRKHCNFVNKKSVE DI SVAS
Sbjct:  258 YFSNLQVKKGLLQSDQELFSTPGADTIPIVNRFSSDQKVF-----FDAFEASMIKMGNIGVLTGKKGEIRKHCNFVNKKSVEVDIASVAS 342

[Close]

BLAST of GR420864 vs. TrEMBL
Match: Q43791_MEDSA (Peroxidase1C OS=Medicago sativa GN=prx1C PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.703e-20
Identity = 59/93 (63.44%), Postives = 66/93 (70.97%), Query Frame = -1

Query:  203 YYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESFKA-----GSIGVITGNKGEIRKHC---NFVNKKSVEQDIVSVAS 457
            YYSNL+VKK       ELFSTSGADTISIVNKFSTDQ+AF     FESFKA     G+IGV+TG KGEIRK C   NFVN  S E D+ ++AS
Sbjct:  258 YYSNLQVKKGLLQSDQELFSTSGADTISIVNKFSTDQNAF-----FESFKAAMIKMGNIGVLTGTKGEIRKQCNFVNFVNSNSAELDLATIAS 345

[Close]

BLAST of GR420864 vs. TrEMBL
Match: C6TI50_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 9.727e-20
Identity = 59/90 (65.56%), Postives = 64/90 (71.11%), Query Frame = -1

Query:  203 YYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESFKA-----GSIGVITGNKGEIRKHCNFVNKKSVEQDIVSVAS 457
            YYSNL+VKK       ELFST GADTISIVNKFS+ Q AF     F+SF A     G+IGV+TG KGEIRK CNFVNKKS E DI SVAS
Sbjct:  256 YYSNLQVKKGLLQSDQELFSTPGADTISIVNKFSSGQIAF-----FKSFSASMIKMGNIGVLTGKKGEIRKQCNFVNKKSAELDIGSVAS 340

[Close]

BLAST of GR420864 vs. TrEMBL
Match: Q8GZR9_LUPAL (Peroxidase 1 (Fragment) OS=Lupinus albus GN=prx1 PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.828e-19
Identity = 54/93 (58.06%), Postives = 65/93 (69.89%), Query Frame = -1

Query:  203 NDKYYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESFKA-----GSIGVITGNKGEIRKHCNFVNKKSVEQDIVSVAS 466
            ++ YYSNL+VKK       ELFST+GADTI++VN F+ +QDAF     F SFKA     G+IGVITG  GEIRK CNF+NKKS E D+ SV S
Sbjct:  193 DNHYYSNLQVKKGLLQSDQELFSTTGADTINLVNTFAKNQDAF-----FASFKASMIKMGNIGVITGKNGEIRKQCNFINKKSAELDLASVVS 280

[Close]

BLAST of GR420864 vs. TrEMBL
Match: Q76KW0_PEA (Peroxidase (Fragment) OS=Pisum sativum GN=PRX PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.834e-18
Identity = 52/81 (64.20%), Postives = 60/81 (74.07%), Query Frame = -1

Query:  230 YYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESF-----KAGSIGVITGNKGEIRKHCNFVNKKSV 457
            YYSNL+VKK       ELFSTSGADTI+IVNKFS D++AF     F+SF     K G+IGV+TGNKGEIRKHCNFVNK  +
Sbjct:  180 YYSNLQVKKGLLQSDQELFSTSGADTITIVNKFSADKNAF-----FDSFETAMIKMGNIGVLTGNKGEIRKHCNFVNKDRI 255

[Close]

BLAST of GR420864 vs. TAIR peptide
Match: AT2G38380.1 (| Symbols: | Peroxidase superfamily protein | chr2:16076443-16078314 FORWARD LENGTH=349)

HSP 1 Score: 57.7658 bits (138), Expect = 3.207e-9
Identity = 37/95 (38.95%), Postives = 55/95 (57.89%), Query Frame = -1

Query:  203 NDKYYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESF-----KAGSIGVITGNKGEIRKHCNFVNK--KSVEQDIVSVAS 466
            + +YY+NL+  K       ELFST GADTI +VN++S+D   F     F +F     + G++  +TG +GEIR++C  VN   + VE D   V+S
Sbjct:  259 DSQYYTNLRNGKGLIQSDQELFSTPGADTIPLVNQYSSDMSVF-----FRAFIDAMIRMGNLRPLTGTQGEIRQNCRVVNPRIRVVENDDGVVSS 348

[Close]

BLAST of GR420864 vs. TAIR peptide
Match: AT5G06730.1 (| Symbols: | Peroxidase superfamily protein | chr5:2080207-2081621 REVERSE LENGTH=358)

HSP 1 Score: 53.5286 bits (127), Expect = 6.049e-8
Identity = 31/83 (37.35%), Postives = 48/83 (57.83%), Query Frame = -1

Query:  233 NDKYYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESF-----KAGSIGVITGNKGEIRKHCNFVNKKS 466
            ++ Y++NL+          ELFS +G+ T+ IVN F+++Q  F     FE+F     K G+I  +TG+ GEIR+ C  VN +S
Sbjct:  260 DNNYFTNLQSNNGLLQSDQELFSNTGSATVPIVNSFASNQTLF-----FEAFVQSMIKMGNISPLTGSSGEIRQDCKVVNGQS 337

[Close]

BLAST of GR420864 vs. TAIR peptide
Match: AT4G08780.1 (| Symbols: | Peroxidase superfamily protein | chr4:5604153-5608202 FORWARD LENGTH=346)

HSP 1 Score: 51.2174 bits (121), Expect = 3.002e-7
Identity = 34/93 (36.56%), Postives = 49/93 (52.69%), Query Frame = -1

Query:  206 NDKYYSNLKVKK*SRGFTSELFST-SGADTISIVNKFSTDQDAFQ*LK*FESF-----KAGSIGVITGNKGEIRKHCNFVNKKSVEQDIVSVA 466
            ++KYY NLK  K       ELFS+   ADT+ +V  ++  Q  F     F++F     +  S+  +TG +GEIR +C  VN KS   D+V  A
Sbjct:  252 DNKYYVNLKENKGLIQSDQELFSSPDAADTLPLVRAYADGQGTF-----FDAFVKAIIRMSSLSPLTGKQGEIRLNCRVVNSKSKIMDVVDDA 339

[Close]

BLAST of GR420864 vs. TAIR peptide
Match: AT4G08770.1 (| Symbols: Prx37 | Peroxidase superfamily protein | chr4:5598259-5600262 REVERSE LENGTH=346)

HSP 1 Score: 50.8322 bits (120), Expect = 3.921e-7
Identity = 34/93 (36.56%), Postives = 50/93 (53.76%), Query Frame = -1

Query:  206 NDKYYSNLKVKK*SRGFTSELFSTSGA-DTISIVNKFSTDQDAFQ*LK*FESF-----KAGSIGVITGNKGEIRKHCNFVNKKSVEQDIVSVA 466
            ++KYY NLK  K       ELFS+  A DT+ +V +++  Q  F     F++F     +  S+  +TG +GEIR +C  VN KS   D+V  A
Sbjct:  252 DNKYYVNLKENKGLIQSDQELFSSPDASDTLPLVREYADGQGKF-----FDAFAKAMIRMSSLSPLTGKQGEIRLNCRVVNSKSKIMDVVEDA 339

[Close]

BLAST of GR420864 vs. TAIR peptide
Match: AT5G19880.1 (| Symbols: | Peroxidase superfamily protein | chr5:6720578-6722411 REVERSE LENGTH=329)

HSP 1 Score: 50.447 bits (119), Expect = 5.121e-7
Identity = 28/80 (35.00%), Postives = 48/80 (60.00%), Query Frame = -1

Query:  242 NDKYYSNLKVKK*SRGFTSELFSTSGADTISIVNKFSTDQDAFQ*LK*FESF-----KAGSIGVITGNKGEIRKHCNFVN 466
            ++ Y+ NL+  +        LFS++GA T+S+VN+F+ +Q+ F     F +F     K G++ ++TG +GEIR+ C  VN
Sbjct:  255 DNDYFKNLQNNRGVIESDQILFSSTGAPTVSLVNRFAENQNEF-----FTNFARSMIKMGNVRILTGREGEIRRDCRRVN 329

[Close]

BLAST of GR420864 vs. TAIR peptide
Match: AT3G32980.1 (| Symbols: | Peroxidase superfamily protein | chr3:13526404-13529949 REVERSE LENGTH=352)

HSP 1 Score: 49.6766 bits (117), Expect = 8.734e-7
Identity = 32/92 (34.78%), Postives = 50/92 (54.35%), Query Frame = -1

Query:  209 NDKYYSNLKVKK*SRGFTSELFSTSGA-DTISIVNKFSTDQDAFQ*LK*FESF-----KAGSIGVITGNKGEIRKHCNFVNKKSVEQDIVSV 466
            ++KYY NLK  K       ELFS+  A DTI +V +++     F     F +F     + G+I  +TG +G+IR++C  VN  S+  D+V +
Sbjct:  259 DNKYYVNLKELKGLIQTDQELFSSPNATDTIPLVREYADGTQKF-----FNAFVEAMNRMGNITPLTGTQGQIRQNCRVVNSNSLLHDVVEI 345

The following BLAST results are available for this feature:

BLAST of GR420864 vs. SwissProt
BLAST of GR420864 vs. TrEMBL
BLAST of GR420864 vs. TAIR peptide

BLAST of GR420864 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
PER15_IPOBA	5.601e-9	45.00	Peroxidase 15 OS=Ipomoea batatas GN=pod PE=1 SV=1	[more]
PER22_ARATH	2.780e-8	38.95	Peroxidase 22 OS=Arabidopsis thaliana GN=PER22 PE=...	[more]
PER3_ARMRU	4.014e-7	40.00	Peroxidase C3 OS=Armoracia rusticana GN=PRXC3 PE=3...	[more]
PER54_ARATH	5.242e-7	37.35	Peroxidase 54 OS=Arabidopsis thaliana GN=PER54 PE=...	[more]

BLAST of GR420864 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q18PQ8_PEA	1.039e-21	65.93	Peroxidase OS=Pisum sativum PE=2 SV=1	[more]
A4UN78_MEDTR	1.357e-21	65.93	Peroxidase OS=Medicago truncatula GN=PRX3 PE=2 SV=...	[more]
C6TB83_SOYBN	3.949e-21	63.33	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q40365_MEDSA	5.158e-21	64.44	Peroxidase OS=Medicago sativa GN=pxdA PE=2 SV=1	[more]
Q43790_MEDSA	8.798e-21	63.33	Peroxidase1B OS=Medicago sativa GN=prx1B PE=2 SV=1	[more]
O23961_SOYBN	1.501e-20	63.33	Peroxidase OS=Glycine max GN=GMIPER1 PE=2 SV=1	[more]
Q43791_MEDSA	5.703e-20	63.44	Peroxidase1C OS=Medicago sativa GN=prx1C PE=2 SV=1	[more]
C6TI50_SOYBN	9.727e-20	65.56	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q8GZR9_LUPAL	4.828e-19	58.06	Peroxidase 1 (Fragment) OS=Lupinus albus GN=prx1 P...	[more]
Q76KW0_PEA	1.834e-18	64.20	Peroxidase (Fragment) OS=Pisum sativum GN=PRX PE=2...	[more]

BLAST of GR420864 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT2G38380.1	3.207e-9	38.95	\| Symbols: \| Peroxidase superfamily protein \| chr...	[more]
AT5G06730.1	6.049e-8	37.35	\| Symbols: \| Peroxidase superfamily protein \| chr...	[more]
AT4G08780.1	3.002e-7	36.56	\| Symbols: \| Peroxidase superfamily protein \| chr...	[more]
AT4G08770.1	3.921e-7	36.56	\| Symbols: Prx37 \| Peroxidase superfamily protein ...	[more]
AT5G19880.1	5.121e-7	35.00	\| Symbols: \| Peroxidase superfamily protein \| chr...	[more]
AT3G32980.1	8.734e-7	34.78	\| Symbols: \| Peroxidase superfamily protein \| chr...	[more]

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR420864 ID=GR420864; Name=GR420864; organism=Cicer arietinum; type=EST; length=466bp
CAAATACAAGTTTTACGAACACAACTCAAAGAAAGATAACACATACCATG
AATGTGTGAAACAATAACTATCTGTTTTCACCCACAAAGACTTATACAGC
TTCTTTATTAGCCTAGTTATGACCTCCAATAGTGACCCTACACTACATTT
CAAGAGTATACATTTACATTGAGCTAACCATACCCTCCTCTGATGAATAT
GTGGAAGCCACACTTACTATGTCTTGTTCTACAGATTTTTTGTTAACAAA
ATTACAATGTTTTCTAATTTCTCCCTTGTTTCCTGTTATGACACCAATAC
TACCAGCTTTAAAGCTTTCAAATCATTTTAATCATTGGAAAGCATCTTGG
TCAGTACTAAACTTGTTGACAATACTAATAGTATCTGCACCAGATGTTGA
AAAAAGCTCTGAAGTAAACCCTCTTGATCACTTTTTAACCTTTAAATTGG
AGTAGTATTTGTCGTT

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