GR913317

EST Overview


Name	GR913317
Unique Name	GR913317
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	221

Libraries


Library Name	Type
LIBEST_024968 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. JG-62) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR913317 vs. SwissProt
Match: NDUA9_BOVIN (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial OS=Bos taurus GN=NDUFA9 PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 1.040e-12
Identity = 35/56 (62.50%), Postives = 42/56 (75.00%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRYVV  L +MGSQ++VP R       HL+ MGDLGQ++ M +N
Sbjct:   54 IVATVFGATGFLGRYVVNHLGRMGSQVIVPHRCEPYDTMHLRPMGDLGQIIFMDWN 109

[Close]

BLAST of GR913317 vs. SwissProt
Match: NDUA9_RAT (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (Fragment) OS=Rattus norvegicus GN=Ndufa9 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.359e-12
Identity = 32/56 (57.14%), Postives = 45/56 (80.36%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            +VATVFGATGFLGRYVV  L +MGSQ+++P+R       HL+LMGDLGQ++ ++++
Sbjct:   47 VVATVFGATGFLGRYVVNHLGRMGSQVIIPYRCDIYDTMHLRLMGDLGQLIFLEWD 102

[Close]

BLAST of GR913317 vs. SwissProt
Match: NDUA9_DICDI (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial OS=Dictyostelium discoideum GN=ndufa9 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.027e-12
Identity = 30/56 (53.57%), Postives = 47/56 (83.93%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            +VATVFGATGF GRY+VQ LA+ G Q++VP+R  ++  R LK++G+LGQ++P++++
Sbjct:   40 LVATVFGATGFTGRYLVQLLARTGIQVVVPYRCEDEGFRDLKVLGELGQIIPVRFD 95

[Close]

BLAST of GR913317 vs. SwissProt
Match: NDUA9_PANTR (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial OS=Pan troglodytes GN=NDUFA9 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 6.743e-12
Identity = 32/56 (57.14%), Postives = 45/56 (80.36%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRYVV  L +MGSQ+++P+R  +    HL+ MGDLGQ++ ++++
Sbjct:   54 IVATVFGATGFLGRYVVNHLGRMGSQVIIPYRCDKYDIMHLRPMGDLGQLLFLEWD 109

[Close]

BLAST of GR913317 vs. SwissProt
Match: NDUA9_HUMAN (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial OS=Homo sapiens GN=NDUFA9 PE=1 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 6.743e-12
Identity = 32/56 (57.14%), Postives = 45/56 (80.36%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRYVV  L +MGSQ+++P+R  +    HL+ MGDLGQ++ ++++
Sbjct:   54 IVATVFGATGFLGRYVVNHLGRMGSQVIIPYRCDKYDIMHLRPMGDLGQLLFLEWD 109

[Close]

BLAST of GR913317 vs. SwissProt
Match: NDUA9_GORGO (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial OS=Gorilla gorilla gorilla GN=NDUFA9 PE=2 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 6.743e-12
Identity = 32/56 (57.14%), Postives = 45/56 (80.36%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRYVV  L +MGSQ+++P+R  +    HL+ MGDLGQ++ ++++
Sbjct:   54 IVATVFGATGFLGRYVVNHLGRMGSQVIIPYRCDKYDIMHLRPMGDLGQLLFLEWD 109

[Close]

BLAST of GR913317 vs. SwissProt
Match: NDUA9_PONPY (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial OS=Pongo pygmaeus GN=NDUFA9 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.150e-11
Identity = 32/56 (57.14%), Postives = 44/56 (78.57%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRYVV  L +MGSQ+++P+R       HL+ MGDLGQ++ ++++
Sbjct:   54 IVATVFGATGFLGRYVVNHLGRMGSQVIIPYRCDTYDIMHLRPMGDLGQLLFLEWD 109

[Close]

BLAST of GR913317 vs. SwissProt
Match: NDUA9_PONAB (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial OS=Pongo abelii GN=NDUFA9 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.150e-11
Identity = 32/56 (57.14%), Postives = 44/56 (78.57%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRYVV  L +MGSQ+++P+R       HL+ MGDLGQ++ ++++
Sbjct:   54 IVATVFGATGFLGRYVVNHLGRMGSQVIIPYRCDTYDIMHLRPMGDLGQLLFLEWD 109

[Close]

BLAST of GR913317 vs. SwissProt
Match: NDUA9_MOUSE (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial OS=Mus musculus GN=Ndufa9 PE=1 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 2.562e-11
Identity = 31/56 (55.36%), Postives = 43/56 (76.79%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            +VATVFGATGFLGRYVV  L +MGSQ+++P+R       HL+LMGD GQ+  ++++
Sbjct:   54 VVATVFGATGFLGRYVVNHLGRMGSQVIIPYRCDVYDIMHLRLMGDPGQLTFLEWD 109

[Close]

BLAST of GR913317 vs. SwissProt
Match: NDUA9_NEUCR (NADH-ubiquinone oxidoreductase 40 kDa subunit, mitochondrial OS=Neurospora crassa GN=nuo-40 PE=1 SV=2)

HSP 1 Score: 62.003 bits (149), Expect = 1.077e-9
Identity = 30/54 (55.56%), Postives = 42/54 (77.78%), Query Frame = 1

Query:   58 ATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            ATVFGATG LGRY+V +LA+ G  +++PFR  E   RHLK+ GDLG+VV ++++
Sbjct:   54 ATVFGATGQLGRYIVNRLARQGCTVVIPFR-DEYNKRHLKVTGDLGKVVMIEFD 106

[Close]

BLAST of GR913317 vs. TrEMBL
Match: Q6K6A4_ORYSJ (Os02g0816800 protein OS=Oryza sativa subsp. japonica GN=P0643F09.23 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 5.530e-23
Identity = 51/56 (91.07%), Postives = 55/56 (98.21%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            I+ATVFGATGFLGRY+VQQLAKMGSQ+LVPFRGSEDC RHLKLMGDLGQ+VPMKYN
Sbjct:   76 IIATVFGATGFLGRYLVQQLAKMGSQVLVPFRGSEDCHRHLKLMGDLGQIVPMKYN 131

[Close]

BLAST of GR913317 vs. TrEMBL
Match: C6TJX1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 5.530e-23
Identity = 52/56 (92.86%), Postives = 55/56 (98.21%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            I+ATVFGATGFLGRYVVQQLAKMGSQ+LVPFRGSED PRHLKLMGDLGQ+VPMKYN
Sbjct:   63 IIATVFGATGFLGRYVVQQLAKMGSQVLVPFRGSEDNPRHLKLMGDLGQIVPMKYN 118

[Close]

BLAST of GR913317 vs. TrEMBL
Match: B8AEU4_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_09443 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 5.530e-23
Identity = 51/56 (91.07%), Postives = 55/56 (98.21%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            I+ATVFGATGFLGRY+VQQLAKMGSQ+LVPFRGSEDC RHLKLMGDLGQ+VPMKYN
Sbjct:   76 IIATVFGATGFLGRYLVQQLAKMGSQVLVPFRGSEDCHRHLKLMGDLGQIVPMKYN 131

[Close]

BLAST of GR913317 vs. TrEMBL
Match: B7FIZ2_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 7.222e-23
Identity = 52/56 (92.86%), Postives = 55/56 (98.21%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRYVVQQLAKMGSQ+LVPFRGSED PRHLKLMGDLGQ+VPMK+N
Sbjct:   63 IVATVFGATGFLGRYVVQQLAKMGSQVLVPFRGSEDSPRHLKLMGDLGQIVPMKFN 118

[Close]

BLAST of GR913317 vs. TrEMBL
Match: B9P8B8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_680727 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 9.433e-23
Identity = 52/56 (92.86%), Postives = 55/56 (98.21%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRYVVQQLAKMGSQ+LVPFRGS+D PRHLKLMGDLGQ+VPMKYN
Sbjct:   61 IVATVFGATGFLGRYVVQQLAKMGSQVLVPFRGSDDDPRHLKLMGDLGQIVPMKYN 116

[Close]

BLAST of GR913317 vs. TrEMBL
Match: B9IBC6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_814393 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 9.433e-23
Identity = 52/56 (92.86%), Postives = 55/56 (98.21%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRYVVQQLAKMGSQ+LVPFRGS+D PRHLKLMGDLGQ+VPMKYN
Sbjct:   61 IVATVFGATGFLGRYVVQQLAKMGSQVLVPFRGSDDDPRHLKLMGDLGQIVPMKYN 116

[Close]

BLAST of GR913317 vs. TrEMBL
Match: A9NUP2_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.609e-22
Identity = 51/56 (91.07%), Postives = 54/56 (96.43%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            I+ATVFGATGFLGRYVVQQLAK GSQ+LVPFRGSED PRHLKLMGDLGQ+VPMKYN
Sbjct:   69 IIATVFGATGFLGRYVVQQLAKTGSQVLVPFRGSEDSPRHLKLMGDLGQIVPMKYN 124

[Close]

BLAST of GR913317 vs. TrEMBL
Match: B9GRC3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_645558 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.744e-22
Identity = 52/56 (92.86%), Postives = 54/56 (96.43%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRYVVQQLAKMGSQ+LVPFRGSED  RHLKLMGDLGQ+VPMKYN
Sbjct:   66 IVATVFGATGFLGRYVVQQLAKMGSQVLVPFRGSEDSHRHLKLMGDLGQIVPMKYN 121

[Close]

BLAST of GR913317 vs. TrEMBL
Match: A9PE76_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.744e-22
Identity = 52/56 (92.86%), Postives = 54/56 (96.43%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRYVVQQLAKMGSQ+LVPFRGSED  RHLKLMGDLGQ+VPMKYN
Sbjct:   66 IVATVFGATGFLGRYVVQQLAKMGSQVLVPFRGSEDSHRHLKLMGDLGQIVPMKYN 121

[Close]

BLAST of GR913317 vs. TrEMBL
Match: Q9SK66_ARATH (Putative NADH-ubiquinone oxireductase OS=Arabidopsis thaliana GN=At2g20360 PE=2 SV=2)

HSP 1 Score: 107.457 bits (267), Expect = 4.681e-22
Identity = 51/56 (91.07%), Postives = 55/56 (98.21%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRY+VQQLAKMGSQ+LVPFRGSED PRHLKLMGDLGQVVPMK++
Sbjct:   69 IVATVFGATGFLGRYLVQQLAKMGSQVLVPFRGSEDSPRHLKLMGDLGQVVPMKFD 124

[Close]

BLAST of GR913317 vs. TAIR peptide
Match: AT2G20360.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr2:8786070-8789098 FORWARD LENGTH=402)

HSP 1 Score: 107.457 bits (267), Expect = 1.801e-24
Identity = 51/56 (91.07%), Postives = 55/56 (98.21%), Query Frame = 1

Query:   52 IVATVFGATGFLGRYVVQQLAKMGSQILVPFRGSEDCPRHLKLMGDLGQVVPMKYN 219
            IVATVFGATGFLGRY+VQQLAKMGSQ+LVPFRGSED PRHLKLMGDLGQVVPMK++
Sbjct:   69 IVATVFGATGFLGRYLVQQLAKMGSQVLVPFRGSEDSPRHLKLMGDLGQVVPMKFD 124

The following BLAST results are available for this feature:

BLAST of GR913317 vs. SwissProt
BLAST of GR913317 vs. TrEMBL
BLAST of GR913317 vs. TAIR peptide

BLAST of GR913317 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NDUA9_BOVIN	1.040e-12	62.50	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
NDUA9_RAT	1.359e-12	57.14	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
NDUA9_DICDI	3.027e-12	53.57	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
NDUA9_PANTR	6.743e-12	57.14	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
NDUA9_HUMAN	6.743e-12	57.14	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
NDUA9_GORGO	6.743e-12	57.14	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
NDUA9_PONPY	1.150e-11	57.14	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
NDUA9_PONAB	1.150e-11	57.14	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
NDUA9_MOUSE	2.562e-11	55.36	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex...	[more]
NDUA9_NEUCR	1.077e-9	55.56	NADH-ubiquinone oxidoreductase 40 kDa subunit, mit...	[more]

BLAST of GR913317 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q6K6A4_ORYSJ	5.530e-23	91.07	Os02g0816800 protein OS=Oryza sativa subsp. japoni...	[more]
C6TJX1_SOYBN	5.530e-23	92.86	Putative uncharacterized protein OS=Glycine max PE...	[more]
B8AEU4_ORYSI	5.530e-23	91.07	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B7FIZ2_MEDTR	7.222e-23	92.86	Putative uncharacterized protein OS=Medicago trunc...	[more]
B9P8B8_POPTR	9.433e-23	92.86	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9IBC6_POPTR	9.433e-23	92.86	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9NUP2_PICSI	1.609e-22	91.07	Putative uncharacterized protein OS=Picea sitchens...	[more]
B9GRC3_POPTR	2.744e-22	92.86	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PE76_POPTR	2.744e-22	92.86	Putative uncharacterized protein OS=Populus tricho...	[more]
Q9SK66_ARATH	4.681e-22	91.07	Putative NADH-ubiquinone oxireductase OS=Arabidops...	[more]

BLAST of GR913317 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G20360.1	1.801e-24	91.07	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 19..53 score: 1.6
None	No IPR available	PANTHER	PTHR12126	NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED	coord: 5..72 score: 4.3
None	No IPR available	PANTHER	PTHR12126:SF1	NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT	coord: 5..72 score: 4.3
None	No IPR available	SEG	seg	seg	coord: 1..15 score
None	No IPR available	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 9..69 score: 7.7

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR913317 ID=GR913317; Name=GR913317; organism=Cicer arietinum; type=EST; length=221bp
GATTAGCGTGGTCGCGGCCGAGGTACTGGTGGACGATCATCTGTCAGTGG
TATTGTTGCTACAGTATTTGGGGCGACTGGATTCCTTGGTCGATATGTTG
TCCAACAACTTGCCAAAATGGGATCTCAAATCCTAGTTCCTTTCCGAGGC
TCTGAAGATTGTCCTCGTCATCTCAAGCTGATGGGAGATTTAGGACAGGT
TGTTCCCATGAAATACAATCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR016040	NAD(P)-bd_dom

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