GR916691

EST Overview


Name	GR916691
Unique Name	GR916691
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	175

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR916691 vs. SwissProt
Match: P2_ARATH (Probable NADP-dependent oxidoreductase P2 OS=Arabidopsis thaliana GN=P2 PE=2 SV=2)

HSP 1 Score: 55.8398 bits (133), Expect = 7.802e-8
Identity = 23/51 (45.10%), Postives = 37/51 (72.55%), Query Frame = 3

Query:   24 YSVVNEQSIVK-KLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            YSV+   + +  K++ ++ PL+Y+ G+LG  G+TAY GF+E+C P+KGE V
Sbjct:  107 YSVITPMAHMHFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETV 157

[Close]

BLAST of GR916691 vs. SwissProt
Match: P1_ARATH (Probable NADP-dependent oxidoreductase P1 OS=Arabidopsis thaliana GN=P1 PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.331e-7
Identity = 24/58 (41.38%), Postives = 39/58 (67.24%), Query Frame = 3

Query:    3 LAWSRPRYSVVNEQSIVK-KLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            +AW    YSV+   +    K++ ++ PL+Y+ G+LG  G+TAY GF+E+C P++GE V
Sbjct:  104 VAWEE--YSVITPMTHAHFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETV 159

[Close]

BLAST of GR916691 vs. TrEMBL
Match: D7SQE9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_52.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00029270001 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.130e-12
Identity = 33/50 (66.00%), Postives = 41/50 (82.00%), Query Frame = 3

Query:   24 YSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            Y++V   +  +KL+   FPL+YHLGVLG SGLTAYGGFFE+CKP+KGEKV
Sbjct:  112 YTIVKGGNTFRKLDPMGFPLSYHLGVLGLSGLTAYGGFFEVCKPKKGEKV 161

[Close]

BLAST of GR916691 vs. TrEMBL
Match: D7TC06_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_16.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00015527001 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.609e-12
Identity = 32/57 (56.14%), Postives = 46/57 (80.70%), Query Frame = 3

Query:    3 LAWSRPRYSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            ++W    YSVV   ++++KL+   FPL+ H+G+LGFSGLTA+GGFF++CKP+KGEKV
Sbjct:  106 ISWGE--YSVVKGGNMLRKLDPMGFPLSNHVGILGFSGLTAHGGFFQVCKPKKGEKV 160

[Close]

BLAST of GR916691 vs. TrEMBL
Match: D7SQE8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_52.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00029269001 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.609e-12
Identity = 32/50 (64.00%), Postives = 41/50 (82.00%), Query Frame = 3

Query:   24 YSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            Y++V   +I++KL+   FPL+YHLGVLG SGLTAY GF E+CKP+KGEKV
Sbjct:  112 YTIVKGGNILRKLDPMGFPLSYHLGVLGLSGLTAYAGFVEVCKPKKGEKV 161

[Close]

BLAST of GR916691 vs. TrEMBL
Match: A5AXT8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_026273 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.609e-12
Identity = 32/57 (56.14%), Postives = 46/57 (80.70%), Query Frame = 3

Query:    3 LAWSRPRYSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            ++W    YSVV   ++++KL+   FPL+ H+G+LGFSGLTA+GGFF++CKP+KGEKV
Sbjct:  108 ISWGE--YSVVKGGNMLRKLDPMGFPLSNHVGILGFSGLTAHGGFFQVCKPKKGEKV 162

[Close]

BLAST of GR916691 vs. TrEMBL
Match: B9SER7_RICCO (Alcohol dehydrogenase, putative OS=Ricinus communis GN=RCOM_0223350 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.131e-11
Identity = 28/50 (56.00%), Postives = 41/50 (82.00%), Query Frame = 3

Query:   24 YSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            YSV+   ++++K +   FP+++H+G+LGFSGLTAY G FE+CKP+KGEKV
Sbjct:  111 YSVIKPGAMLRKFDPMRFPMSHHVGILGFSGLTAYAGLFEVCKPKKGEKV 160

[Close]

BLAST of GR916691 vs. TrEMBL
Match: A5BJX1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_003098 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.131e-11
Identity = 31/50 (62.00%), Postives = 40/50 (80.00%), Query Frame = 3

Query:   24 YSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            Y++V   + ++KL+   FPL+YHLGVLG SGLTAY GF E+CKP+KGEKV
Sbjct:  112 YTIVKGGNTLRKLDPMGFPLSYHLGVLGLSGLTAYAGFVEVCKPKKGEKV 161

[Close]

BLAST of GR916691 vs. TrEMBL
Match: B9SER6_RICCO (Alcohol dehydrogenase, putative OS=Ricinus communis GN=RCOM_0223340 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.606e-9
Identity = 28/57 (49.12%), Postives = 40/57 (70.18%), Query Frame = 3

Query:    3 LAWSRPRYSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            ++W    YS+     ++ KL+    PL++H+G+LG+SGLTAY G F+ICKP KGEKV
Sbjct:  107 ISWGE--YSIRKSPGLLTKLDPIGLPLSHHVGILGYSGLTAYAGLFDICKPMKGEKV 161

[Close]

BLAST of GR916691 vs. TrEMBL
Match: B9HAL1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_560614 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.606e-9
Identity = 30/51 (58.82%), Postives = 39/51 (76.47%), Query Frame = 3

Query:   24 YSVVNEQSIVKKLE-SSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            YSVV    +++K++  +E PL+YH G LG SGLTAY G FEICKP+KG+KV
Sbjct:  111 YSVVKGGGMLRKVDPKTELPLSYHAGSLGLSGLTAYAGLFEICKPKKGDKV 161

[Close]

BLAST of GR916691 vs. TrEMBL
Match: Q8S0M7_ORYSJ (Os01g0891300 protein OS=Oryza sativa subsp. japonica GN=B1078G07.5 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.403e-9
Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 3

Query:   24 YSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            YS+++    + K++ S+ PL+YHLG+LG  G TAY GF+EIC P+KGE V
Sbjct:  118 YSLIDRPEQLSKIQQSDIPLSYHLGLLGMPGFTAYAGFYEICSPKKGEFV 167

[Close]

BLAST of GR916691 vs. TrEMBL
Match: B8A7E3_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_04748 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.403e-9
Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 3

Query:   24 YSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            YS+++    + K++ S+ PL+YHLG+LG  G TAY GF+EIC P+KGE V
Sbjct:  110 YSLIDRPEQLSKIQQSDIPLSYHLGLLGMPGFTAYAGFYEICSPKKGEFV 159

[Close]

BLAST of GR916691 vs. TAIR peptide
Match: AT3G59845.2 (| Symbols: | Zinc-binding dehydrogenase family protein | chr3:22106421-22107407 REVERSE LENGTH=244)

HSP 1 Score: 56.9954 bits (136), Expect = 2.803e-9
Identity = 25/50 (50.00%), Postives = 34/50 (68.00%), Query Frame = 3

Query:   24 YSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            +S +N  +I K   +   PL+Y+ G+LG  GLTAY GFFEIC P+KG+ V
Sbjct:   11 FSTINPAAIFKIDVNINVPLSYYTGILGMIGLTAYAGFFEICSPKKGDTV 60

[Close]

BLAST of GR916691 vs. TAIR peptide
Match: AT3G03080.1 (| Symbols: | Zinc-binding dehydrogenase family protein | chr3:698530-700278 REVERSE LENGTH=350)

HSP 1 Score: 56.9954 bits (136), Expect = 2.803e-9
Identity = 23/50 (46.00%), Postives = 35/50 (70.00%), Query Frame = 3

Query:   24 YSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            YS++       K++ ++ PL+Y+ G+LG  G+TAY GF+EIC P+KGE V
Sbjct:  115 YSLITPDFSHYKIQHTDVPLSYYTGLLGMPGMTAYAGFYEICSPKKGETV 164

[Close]

BLAST of GR916691 vs. TAIR peptide
Match: AT3G59845.1 (| Symbols: | Zinc-binding dehydrogenase family protein | chr3:22106421-22107785 REVERSE LENGTH=346)

HSP 1 Score: 56.9954 bits (136), Expect = 2.803e-9
Identity = 25/50 (50.00%), Postives = 34/50 (68.00%), Query Frame = 3

Query:   24 YSVVNEQSIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            +S +N  +I K   +   PL+Y+ G+LG  GLTAY GFFEIC P+KG+ V
Sbjct:  113 FSTINPAAIFKIDVNINVPLSYYTGILGMIGLTAYAGFFEICSPKKGDTV 162

[Close]

BLAST of GR916691 vs. TAIR peptide
Match: AT5G16990.1 (| Symbols: | Zinc-binding dehydrogenase family protein | chr5:5581831-5583849 REVERSE LENGTH=343)

HSP 1 Score: 55.8398 bits (133), Expect = 6.244e-9
Identity = 23/51 (45.10%), Postives = 37/51 (72.55%), Query Frame = 3

Query:   24 YSVVNEQSIVK-KLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            YSV+   + +  K++ ++ PL+Y+ G+LG  G+TAY GF+E+C P+KGE V
Sbjct:  107 YSVITPMAHMHFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETV 157

[Close]

BLAST of GR916691 vs. TAIR peptide
Match: AT1G26320.2 (| Symbols: | Zinc-binding dehydrogenase family protein | chr1:9105240-9107029 FORWARD LENGTH=346)

HSP 1 Score: 55.8398 bits (133), Expect = 6.244e-9
Identity = 21/39 (53.85%), Postives = 31/39 (79.49%), Query Frame = 3

Query:   57 KLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            K+E ++ PL+Y+ G+LG  G+TAY GF+E+C P+KGE V
Sbjct:  127 KIEHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETV 165

[Close]

BLAST of GR916691 vs. TAIR peptide
Match: AT1G26320.1 (| Symbols: | Zinc-binding dehydrogenase family protein | chr1:9105240-9107029 FORWARD LENGTH=351)

HSP 1 Score: 55.8398 bits (133), Expect = 6.244e-9
Identity = 21/39 (53.85%), Postives = 31/39 (79.49%), Query Frame = 3

Query:   57 KLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            K+E ++ PL+Y+ G+LG  G+TAY GF+E+C P+KGE V
Sbjct:  127 KIEHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETV 165

[Close]

BLAST of GR916691 vs. TAIR peptide
Match: AT5G16970.1 (| Symbols: AT-AER, AER | alkenal reductase | chr5:5576291-5578001 REVERSE LENGTH=345)

HSP 1 Score: 55.0694 bits (131), Expect = 1.065e-8
Identity = 24/58 (41.38%), Postives = 39/58 (67.24%), Query Frame = 3

Query:    3 LAWSRPRYSVVNEQSIVK-KLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            +AW    YSV+   +    K++ ++ PL+Y+ G+LG  G+TAY GF+E+C P++GE V
Sbjct:  104 VAWEE--YSVITPMTHAHFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETV 159

[Close]

BLAST of GR916691 vs. TAIR peptide
Match: AT5G17000.1 (| Symbols: | Zinc-binding dehydrogenase family protein | chr5:5584983-5586991 REVERSE LENGTH=345)

HSP 1 Score: 54.6842 bits (130), Expect = 1.391e-8
Identity = 22/51 (43.14%), Postives = 37/51 (72.55%), Query Frame = 3

Query:   24 YSVVNEQSIVK-KLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            YSV+   + +  K++ ++ PL+Y+ G+LG  G+TAY GF+E+C P++GE V
Sbjct:  109 YSVITPMTHMHFKIQHTDIPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETV 159

[Close]

BLAST of GR916691 vs. TAIR peptide
Match: AT5G16980.2 (| Symbols: | Zinc-binding dehydrogenase family protein | chr5:5579202-5580889 REVERSE LENGTH=305)

HSP 1 Score: 53.9138 bits (128), Expect = 2.373e-8
Identity = 21/39 (53.85%), Postives = 31/39 (79.49%), Query Frame = 3

Query:   57 KLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            K++ ++ PL+Y+ G+LG  G+TAY GF+EIC P+KGE V
Sbjct:   81 KIQHTDVPLSYYTGLLGMPGMTAYVGFYEICSPKKGETV 119

[Close]

BLAST of GR916691 vs. TAIR peptide
Match: AT5G37940.1 (| Symbols: | Zinc-binding dehydrogenase family protein | chr5:15107312-15108820 FORWARD LENGTH=353)

HSP 1 Score: 53.9138 bits (128), Expect = 2.373e-8
Identity = 28/61 (45.90%), Postives = 38/61 (62.30%), Query Frame = 3

Query:    3 LAWSR---PRYSVVNEQ-SIVKKLESSEFPLTYHLGVLGFSGLTAYGGFFEICKPRKGEKV 173
            L W R     YSV+    S   K+  ++ PL+++ G+LG  GLTAY GF+EIC P+KGE V
Sbjct:  107 LLWGRVGWEEYSVITPTPSSHFKIHHTDVPLSFYTGLLGIPGLTAYVGFYEICSPKKGETV 167

The following BLAST results are available for this feature:

BLAST of GR916691 vs. SwissProt
BLAST of GR916691 vs. TrEMBL
BLAST of GR916691 vs. TAIR peptide

BLAST of GR916691 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
P2_ARATH	7.802e-8	45.10	Probable NADP-dependent oxidoreductase P2 OS=Arabi...	[more]
P1_ARATH	1.331e-7	41.38	Probable NADP-dependent oxidoreductase P1 OS=Arabi...	[more]

BLAST of GR916691 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7SQE9_VITVI	1.130e-12	66.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7TC06_VITVI	5.609e-12	56.14	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7SQE8_VITVI	5.609e-12	64.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AXT8_VITVI	5.609e-12	56.14	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9SER7_RICCO	2.131e-11	56.00	Alcohol dehydrogenase, putative OS=Ricinus communi...	[more]
A5BJX1_VITVI	2.131e-11	62.00	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9SER6_RICCO	2.606e-9	49.12	Alcohol dehydrogenase, putative OS=Ricinus communi...	[more]
B9HAL1_POPTR	2.606e-9	58.82	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q8S0M7_ORYSJ	3.403e-9	52.00	Os01g0891300 protein OS=Oryza sativa subsp. japoni...	[more]
B8A7E3_ORYSI	3.403e-9	52.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GR916691 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G59845.2	2.803e-9	50.00	\| Symbols: \| Zinc-binding dehydrogenase family pr...	[more]
AT3G03080.1	2.803e-9	46.00	\| Symbols: \| Zinc-binding dehydrogenase family pr...	[more]
AT3G59845.1	2.803e-9	50.00	\| Symbols: \| Zinc-binding dehydrogenase family pr...	[more]
AT5G16990.1	6.244e-9	45.10	\| Symbols: \| Zinc-binding dehydrogenase family pr...	[more]
AT1G26320.2	6.244e-9	53.85	\| Symbols: \| Zinc-binding dehydrogenase family pr...	[more]
AT1G26320.1	6.244e-9	53.85	\| Symbols: \| Zinc-binding dehydrogenase family pr...	[more]
AT5G16970.1	1.065e-8	41.38	\| Symbols: AT-AER, AER \| alkenal reductase \| chr5:...	[more]
AT5G17000.1	1.391e-8	43.14	\| Symbols: \| Zinc-binding dehydrogenase family pr...	[more]
AT5G16980.2	2.373e-8	53.85	\| Symbols: \| Zinc-binding dehydrogenase family pr...	[more]
AT5G37940.1	2.373e-8	45.90	\| Symbols: \| Zinc-binding dehydrogenase family pr...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002085	Alcohol dehydrogenase superfamily, zinc-containing	PANTHER	PTHR11695	ALCOHOL DEHYDROGENASE RELATED	coord: 8..57 score: 2.4
None	No IPR available	GENE3D	3.90.180.10	no description	coord: 7..57 score: 1.7
None	No IPR available	PANTHER	PTHR11695:SF5	ALCOHOL DEHYDROGENASE	coord: 8..57 score: 2.4

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR916691 ID=GR916691; Name=GR916691; organism=Cicer arietinum; type=EST; length=175bp
GATTAGCGTGGTCGCGGCCGAGGTACAGTGTGGTTAACGAGCAAAGCATA
GTAAAAAAACTAGAGTCCTCTGAATTTCCACTCACTTACCATCTAGGAGT
TCTAGGATTTAGTGGACTGACAGCTTATGGGGGATTTTTCGAAATTTGCA
AACCCCGAAAGGGTGAAAAAGTATT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008270	zinc ion binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR002085	ADH_SF_Zn-type

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