HO664251

EST Overview


Name	HO664251
Unique Name	HO664251
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	302

Libraries


Library Name	Type
LIBEST_026619 TDZ treated Chickpea SSH library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO664251 vs. SwissProt
Match: ADAL_DROME (Adenosine deaminase-like protein OS=Drosophila melanogaster GN=Ada PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.448e-9
Identity = 33/82 (40.24%), Postives = 49/82 (59.76%), Query Frame = 1

Query:   46 KCNANARKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFG---VVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGE 282
            K  +    ++ V+LL SI+R E  + A ETV LA+E+       ++GIDLSGNP  G +  F P L  A+++GL + +HC E
Sbjct:  120 KAASETYPEITVKLLPSINRAEPVDVAEETVSLAVELARAHPNLILGIDLSGNPGKGRFSDFAPILAQARDKGLKLAIHCAE 201

[Close]

BLAST of HO664251 vs. SwissProt
Match: ADAL_DROPS (Adenosine deaminase-like protein OS=Drosophila pseudoobscura pseudoobscura GN=GA11319 PE=3 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 7.123e-9
Identity = 37/80 (46.25%), Postives = 47/80 (58.75%), Query Frame = 1

Query:   61 ARKK---VFVRLLLSIDRRETTEAAMETVMLALEMRHFG---VVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGE 282
            +RKK   + V+LL SI+R E    A ETV LALE        VVGIDLSG P  G++  F  AL  A+ +GL + +HC E
Sbjct:  124 SRKKYPNILVKLLPSINRSEPVAVAEETVALALEFAKTDPDLVVGIDLSGIPTKGKFTDFCGALDLARREGLKLVIHCAE 203

[Close]

BLAST of HO664251 vs. SwissProt
Match: ADAL_DANRE (Adenosine deaminase-like protein OS=Danio rerio GN=adal PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 3.535e-8
Identity = 34/75 (45.33%), Postives = 45/75 (60.00%), Query Frame = 1

Query:   79 VRLLLSIDRRETTEAAMETVMLA---LEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAPAR 294
            VR L+++DRR   E AM+TV LA   L      VVG+DLSG+P VG     L AL+ A+  GL + LH  E P++
Sbjct:  135 VRFLVAVDRRHGPEVAMQTVKLAEDFLLSSDGTVVGLDLSGDPTVGHGKDLLAALQKAKNCGLKLALHLSEVPSQ 209

[Close]

BLAST of HO664251 vs. SwissProt
Match: ADALA_XENLA (Adenosine deaminase-like protein A OS=Xenopus laevis GN=adal-a PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.754e-7
Identity = 33/75 (44.00%), Postives = 45/75 (60.00%), Query Frame = 1

Query:   79 VRLLLSIDRRETTEAAMETVMLALEM---RHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAPAR 294
            VR LL+IDRR    AA ETV LA +     +  V+G+DLSG+P VG    F+  L  A++ GL + LH  E P++
Sbjct:  135 VRFLLAIDRRGGPTAAKETVKLAEDFFCSSNELVLGLDLSGDPTVGHGRDFMEPLNKARQSGLKLALHLSEIPSQ 209

[Close]

BLAST of HO664251 vs. SwissProt
Match: ADAL_BOVIN (Adenosine deaminase-like protein OS=Bos taurus GN=ADAL PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 2.993e-7
Identity = 33/73 (45.21%), Postives = 43/73 (58.90%), Query Frame = 1

Query:   79 VRLLLSIDRRETTEAAMETVMLALEM---RHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAP 288
            VR L+SIDRR  + AA E V LA E        V+G+DLSG+P+ G+   FL  L  A++ GL + LH  E P
Sbjct:  140 VRYLISIDRRGGSSAAKEAVKLAEEFFLSAEDTVLGLDLSGDPSAGQAKDFLEPLLEAKKSGLKLALHLSEIP 212

[Close]

BLAST of HO664251 vs. SwissProt
Match: ADAL_MOUSE (Adenosine deaminase-like protein OS=Mus musculus GN=Adal PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 6.667e-7
Identity = 33/75 (44.00%), Postives = 43/75 (57.33%), Query Frame = 1

Query:   79 VRLLLSIDRRETTEAAMETVMLALEM---RHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAPAR 294
            VR L++IDRR     A ETV LA E        V+G+DLSG+P +G+   FL  L  A++ GL + LH  E P R
Sbjct:  140 VRYLMAIDRRGGPTIARETVELAKEFFLSTENTVLGLDLSGDPTIGQANDFLEPLLEAKKAGLKLALHLAEIPNR 214

[Close]

BLAST of HO664251 vs. TrEMBL
Match: B9RXP2_RICCO (Adenosine deaminase, putative OS=Ricinus communis GN=RCOM_0905310 PE=3 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.847e-34
Identity = 70/85 (82.35%), Postives = 76/85 (89.41%), Query Frame = 1

Query:   40 NDKCNANARKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAPAR 294
            ND C  + R+K++VRLLLSIDRRETTEAAMETV LALEMRH GVVGIDLSGNP VGEW+TFLPALKFAQEQGLY+TLHCGE P R
Sbjct:  147 NDACCKSTRRKIYVRLLLSIDRRETTEAAMETVKLALEMRHLGVVGIDLSGNPVVGEWITFLPALKFAQEQGLYITLHCGEVPNR 231

[Close]

BLAST of HO664251 vs. TrEMBL
Match: B9P4P9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_837657 PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 1.912e-31
Identity = 66/81 (81.48%), Postives = 72/81 (88.89%), Query Frame = 1

Query:   40 NDKCNANARKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGE 282
            ND C+   +KK++VRLLLSIDRRETTEAA+ETV LALEMR  GVVGIDLSGNP VGEW TFLPALKFAQEQGLY+TLHCGE
Sbjct:  147 NDACDGTKKKKIYVRLLLSIDRRETTEAAIETVKLALEMRDLGVVGIDLSGNPVVGEWNTFLPALKFAQEQGLYITLHCGE 227

[Close]

BLAST of HO664251 vs. TrEMBL
Match: C6TC39_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 1.618e-30
Identity = 63/79 (79.75%), Postives = 74/79 (93.67%), Query Frame = 1

Query:   52 NANARKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAP 288
            + +ARK++FVRLLLSIDRRETTEAAM+TV LALEMR +GVVGIDLSGNP++G+W T+LPALKFA+EQGLYVTLHCGE P
Sbjct:  148 DGHARKRIFVRLLLSIDRRETTEAAMDTVKLALEMRPYGVVGIDLSGNPSIGDWTTYLPALKFAREQGLYVTLHCGELP 226

[Close]

BLAST of HO664251 vs. TrEMBL
Match: A5C4M9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030807 PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.114e-30
Identity = 65/83 (78.31%), Postives = 71/83 (85.54%), Query Frame = 1

Query:   40 NDKCNANARKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAP 288
            ND CN   +KK+FVRLLLSIDRRETT  AMETV LALEMR  GVVGIDLSGNP VG+W+TFLPALKFA+EQGL +TLHCGE P
Sbjct:  139 NDICNGTTKKKIFVRLLLSIDRRETTADAMETVKLALEMRDQGVVGIDLSGNPTVGDWMTFLPALKFAREQGLSITLHCGEVP 221

[Close]

BLAST of HO664251 vs. TrEMBL
Match: D7M1U8_ARALY (Adenosine/AMP deaminase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_911747 PE=4 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 5.756e-28
Identity = 60/80 (75.00%), Postives = 69/80 (86.25%), Query Frame = 1

Query:   49 CNANARKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAP 288
            C+   RKK++VRLLLSIDRRETTE+AMETV LALEMR  GVVGIDLSGNP VGEW TFLPAL+FA++  L++TLHCGE P
Sbjct:  143 CDGIGRKKIYVRLLLSIDRRETTESAMETVKLALEMRDVGVVGIDLSGNPLVGEWSTFLPALQFAKDSNLHITLHCGEVP 222

[Close]

BLAST of HO664251 vs. TrEMBL
Match: Q8LPL7_ARATH (Putative adenosine deaminase OS=Arabidopsis thaliana GN=At4g04880 PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.086e-26
Identity = 58/75 (77.33%), Postives = 67/75 (89.33%), Query Frame = 1

Query:   64 RKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAP 288
            RKK++VRLLLSIDRRETTE+AMETV LALEMR  GVVGIDLSGNP VGEW TFLPAL++A++  L++TLHCGE P
Sbjct:  148 RKKIYVRLLLSIDRRETTESAMETVKLALEMRDVGVVGIDLSGNPLVGEWSTFLPALQYAKDNDLHITLHCGEVP 222

[Close]

BLAST of HO664251 vs. TrEMBL
Match: Q8H3U7_ORYSJ (Os07g0661000 protein OS=Oryza sativa subsp. japonica GN=P0496C02.124 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.242e-22
Identity = 51/77 (66.23%), Postives = 65/77 (84.42%), Query Frame = 1

Query:   64 RKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAPAR 294
            +KK++VRLLLSIDRRETT AA++TV LA+EM+  GV+GIDLSGNP VGEW T+LPAL+ A+E G+ +T+HCGE   R
Sbjct:  160 KKKIYVRLLLSIDRRETTLAALDTVNLAMEMKDQGVIGIDLSGNPVVGEWETYLPALEHAKELGIPITIHCGEVANR 236

[Close]

BLAST of HO664251 vs. TrEMBL
Match: A3BN30_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_25451 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.242e-22
Identity = 51/77 (66.23%), Postives = 65/77 (84.42%), Query Frame = 1

Query:   64 RKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAPAR 294
            +KK++VRLLLSIDRRETT AA++TV LA+EM+  GV+GIDLSGNP VGEW T+LPAL+ A+E G+ +T+HCGE   R
Sbjct:  134 KKKIYVRLLLSIDRRETTLAALDTVNLAMEMKDQGVIGIDLSGNPVVGEWETYLPALEHAKELGIPITIHCGEVANR 210

[Close]

BLAST of HO664251 vs. TrEMBL
Match: B4FH67_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.767e-22
Identity = 51/77 (66.23%), Postives = 63/77 (81.82%), Query Frame = 1

Query:   64 RKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAPAR 294
            +KK++VRLLLSIDRRETT AA++TV LA+EM   GV+GIDLSGNP VGEW T+LPAL+ A+  G+ VT+HCGE   R
Sbjct:  167 KKKIYVRLLLSIDRRETTSAALDTVNLAVEMMDQGVIGIDLSGNPVVGEWETYLPALQHAKNMGIPVTIHCGEVANR 243

[Close]

BLAST of HO664251 vs. TrEMBL
Match: B6TRX4_MAIZE (Deaminase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 3.614e-22
Identity = 51/77 (66.23%), Postives = 63/77 (81.82%), Query Frame = 1

Query:   64 RKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAPAR 294
            +KK++VRLLLSIDRRETT AA++TV LA+EM   GV+GIDLSGNP VGEW T+LPAL+ A+  G+ VT+HCGE   R
Sbjct:  167 KKKIYVRLLLSIDRRETTSAALDTVNLAMEMMDQGVIGIDLSGNPVVGEWETYLPALQHAKNLGIPVTIHCGEVANR 243

[Close]

BLAST of HO664251 vs. TAIR peptide
Match: AT4G04880.1 (| Symbols: | adenosine/AMP deaminase family protein | chr4:2465055-2467103 REVERSE LENGTH=355)

HSP 1 Score: 122.865 bits (307), Expect = 4.134e-29
Identity = 58/75 (77.33%), Postives = 67/75 (89.33%), Query Frame = 1

Query:   64 RKKVFVRLLLSIDRRETTEAAMETVMLALEMRHFGVVGIDLSGNPAVGEWVTFLPALKFAQEQGLYVTLHCGEAP 288
            RKK++VRLLLSIDRRETTE+AMETV LALEMR  GVVGIDLSGNP VGEW TFLPAL++A++  L++TLHCGE P
Sbjct:  148 RKKIYVRLLLSIDRRETTESAMETVKLALEMRDVGVVGIDLSGNPLVGEWSTFLPALQYAKDNDLHITLHCGEVP 222

The following BLAST results are available for this feature:

BLAST of HO664251 vs. SwissProt
BLAST of HO664251 vs. TrEMBL
BLAST of HO664251 vs. TAIR peptide

BLAST of HO664251 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 6


Match Name	E-value	Identity	Description
ADAL_DROME	2.448e-9	40.24	Adenosine deaminase-like protein OS=Drosophila mel...	[more]
ADAL_DROPS	7.123e-9	46.25	Adenosine deaminase-like protein OS=Drosophila pse...	[more]
ADAL_DANRE	3.535e-8	45.33	Adenosine deaminase-like protein OS=Danio rerio GN...	[more]
ADALA_XENLA	1.754e-7	44.00	Adenosine deaminase-like protein A OS=Xenopus laev...	[more]
ADAL_BOVIN	2.993e-7	45.21	Adenosine deaminase-like protein OS=Bos taurus GN=...	[more]
ADAL_MOUSE	6.667e-7	44.00	Adenosine deaminase-like protein OS=Mus musculus G...	[more]

BLAST of HO664251 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9RXP2_RICCO	1.847e-34	82.35	Adenosine deaminase, putative OS=Ricinus communis ...	[more]
B9P4P9_POPTR	1.912e-31	81.48	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C6TC39_SOYBN	1.618e-30	79.75	Putative uncharacterized protein OS=Glycine max PE...	[more]
A5C4M9_VITVI	2.114e-30	78.31	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7M1U8_ARALY	5.756e-28	75.00	Adenosine/AMP deaminase family protein OS=Arabidop...	[more]
Q8LPL7_ARATH	1.086e-26	77.33	Putative adenosine deaminase OS=Arabidopsis thalia...	[more]
Q8H3U7_ORYSJ	1.242e-22	66.23	Os07g0661000 protein OS=Oryza sativa subsp. japoni...	[more]
A3BN30_ORYSJ	1.242e-22	66.23	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B4FH67_MAIZE	2.767e-22	66.23	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6TRX4_MAIZE	3.614e-22	66.23	Deaminase OS=Zea mays PE=2 SV=1	[more]

BLAST of HO664251 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G04880.1	4.134e-29	77.33	\| Symbols: \| adenosine/AMP deaminase family prote...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001365	Adenosine/AMP deaminase	PANTHER	PTHR11409	ADENOSINE DEAMINASE	coord: 10..96 score: 2.2
IPR001365	Adenosine/AMP deaminase	PFAM	PF00962	A_deaminase	coord: 30..95 score: 3.1
None	No IPR available	GENE3D	3.20.20.140	no description	coord: 19..96 score: 3.4
None	No IPR available	PANTHER	PTHR11409:SF21	ADENOSINE DEAMINASE	coord: 10..96 score: 2.2
None	No IPR available	SUPERFAMILY	51556	Metallo-dependent hydrolases	coord: 19..95 score: 3.9

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO664251 ID=HO664251; Name=HO664251; organism=Cicer arietinum; type=EST; length=302bp
AGCGTGGTCGCGGCCGAGGTACTCCCAGTCTCAGGCACAAATGATAAATG
TAATGCAAATGCTAGGAAAAAAGTATTTGTCAGGCTTCTCTTAAGCATTG
ATCGTAGGGAGACAACAGAAGCAGCAATGGAAACTGTCATGCTTGCGCTA
GAAATGAGGCATTTTGGGGTCGTTGGAATTGACCTCTCTGGGAATCCAGC
TGTTGGTGAATGGGTTACATTTTTGCCAGCATTAAAATTTGCTCAAGAAC
AAGGTCTTTATGTAACTCTTCATTGTGGAGAGGCACCTGCCCGGGCGGCC
GC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0009168	purine ribonucleoside monophosphate biosynthetic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0019239	deaminase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR001365	A_deaminase_dom

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