GR391615

EST Overview
NameGR391615
Unique NameGR391615
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length775
Libraries
Library NameType
LIBEST_024777 ICC1882 dehydration stressed root cDNA librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of GR391615 vs. SwissProt
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 182.57 bits (462), Expect = 1.306e-51
Identity = 87/123 (70.73%), Postives = 97/123 (78.86%), Query Frame = 1 
Query:  112 VIRLLFQSRKFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCI 480
            +  ++ ++RKFK               HHLKRPLHRTFKGLS+ YG + SLW+GSRLVVVVSS  EFQQCFTKNDVVL+NRPRFLSGKYIFYNYTTLGSTSY EHWRNLRRIT+LDVLS H I
Sbjct:   19 IFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRI 141

HSP 2 Score: 42.3578 bits (98), Expect = 1.306e-51
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2 
Query:  470 NTASKNFPGARRDENHRLITKLAEDSSTSFAE 565
            N    +F G RRDE  RLIT+LA+DSST+FAE
Sbjct:  138 NHRINSFSGIRRDETQRLITRLADDSSTNFAE 169
[Close]
BLAST of GR391615 vs. SwissProt
Match: C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 100.908 bits (250), Expect = 8.252e-21
Identity = 49/95 (51.58%), Postives = 67/95 (70.53%), Query Frame = 1 
Query:  193 HHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFT-KNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            H +K P+HR  +  S  YG I SL +GSR VVV++S    Q+ FT +ND+VLS+RP  L+ KY+ YN+TT+G+  Y +HWRNLRRI S ++LS H
Sbjct:   42 HLMKPPIHRLLQRYSNQYGPIFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDHWRNLRRICSQEILSSH 136
[Close]
BLAST of GR391615 vs. SwissProt
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 5.006e-18
Identity = 46/98 (46.94%), Postives = 66/98 (67.35%), Query Frame = 1 
Query:  199 LKRPLHRTFKGLSKTY-----GDIISLWYGSRLVVVVSSLP-EFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            LK P+HRT +  S+T      G ++SL  GSRLV VVSS     ++CF KNDVVL+NRP+ + GK++ YN T + +  Y +HWRNLRR+ ++++ S H
Sbjct:   50 LKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLRRLCTIEIFSTH 147
[Close]
BLAST of GR391615 vs. SwissProt
Match: C75A1_PINTA (Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.507e-14
Identity = 37/97 (38.14%), Postives = 60/97 (61.86%), Query Frame = 1 
Query:  193 HHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCIK 483
            H ++ PLHRT K L++ YG I+ L +GS   VVVSS  + +     +D++ ++RP    GKY FYN+  +  + Y +HWR +R+I  L++L+   I+
Sbjct:   56 HQVRLPLHRTLKNLAEKYGPILFLRFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMRKICVLELLTSKRIE 152
[Close]
BLAST of GR391615 vs. SwissProt
Match: C82A1_PEA (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 1.560e-11
Identity = 33/92 (35.87%), Postives = 57/92 (61.96%), Query Frame = 1 
Query:  214 HRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCIKKL 489
            H+T   L   YG I ++  G+   +V+S+    ++CFTKND+V+S+RP+ ++ + + YN   +G   Y  +WR LR+I +L++LS   I+ L
Sbjct:   58 HKTLGALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYGAYWRQLRKIVTLEILSNRRIELL 149
[Close]
BLAST of GR391615 vs. SwissProt
Match: C71DB_LOTJA (Cytochrome P450 71D11 (Fragment) OS=Lotus japonicus GN=CYP71D11 PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 7.742e-11
Identity = 31/99 (31.31%), Postives = 57/99 (57.58%), Query Frame = 1 
Query:  193 HHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCIKKL 489
            H +  P HR  + L+K YG ++ L  G  + ++VSS    ++    +DV  ++RPR L    +FY  T +G + Y ++WR +R+I ++++LS   ++ L
Sbjct:   34 HLVGSPPHRKLRDLAKKYGPLMHLQLGEVIFIIVSSAEYAKEVMKTHDVTFASRPRSLFTDIVFYGSTDIGFSPYGDYWRQVRKICNVELLSMKRVQSL 132
[Close]
BLAST of GR391615 vs. SwissProt
Match: C71BM_ARATH (Cytochrome P450 71B22 OS=Arabidopsis thaliana GN=CYP71B22 PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 7.742e-11
Identity = 32/97 (32.99%), Postives = 53/97 (54.64%), Query Frame = 1 
Query:  193 HHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCIK 483
            H L + LHR+F  LS+ YG ++ L +G   VVVVS+    ++    +D+    RP+  + K   YNY  +G   Y + WR +R++  L++ S   +K
Sbjct:   42 HQLGKSLHRSFHKLSQNYGPVMFLHFGVVPVVVVSTREAAEEVLKTHDLETCTRPKLTATKLFSYNYKDIGFAQYGDDWREMRKLAMLELFSSKKLK 138
[Close]
BLAST of GR391615 vs. SwissProt
Match: C82A2_SOYBN (Cytochrome P450 82A2 OS=Glycine max GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.907e-9
Identity = 30/92 (32.61%), Postives = 52/92 (56.52%), Query Frame = 1 
Query:  214 HRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCIKKL 489
            H+T   L+  YG I S+  G++  VVVS+    ++C+T ND+ +S+ P  +S   + YN + +    Y  +WR LR+I   + LS   +++L
Sbjct:   60 HKTLGDLADKYGPIFSIKIGAKNAVVVSNWEMAKECYTTNDIAVSSLPDLISANLLCYNRSMIVVAPYGPYWRQLRKILMSEFLSPSRVEQL 151
[Close]
BLAST of GR391615 vs. SwissProt
Match: C82A4_SOYBN (Cytochrome P450 82A4 OS=Glycine max GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.253e-9
Identity = 29/92 (31.52%), Postives = 52/92 (56.52%), Query Frame = 1 
Query:  214 HRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCIKKL 489
            H+    L++ +G + ++  G++  +VVS     ++CFT NDV +S RP+ L  + + YN   L    Y  +WR LR+I   ++LS   +++L
Sbjct:   63 HKALGALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVAPYGPYWRELRKIIVTEILSSSRVEQL 154
[Close]
BLAST of GR391615 vs. SwissProt
Match: C71A3_SOLME (Cytochrome P450 71A3 (Fragment) OS=Solanum melongena GN=CYP71A3 PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.253e-9
Identity = 33/92 (35.87%), Postives = 49/92 (53.26%), Query Frame = 1 
Query:  193 HHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLS 468
            H L    HR+   LSK YG ++ L  GS+ V+V SS+   +     ND+V SNRP+      + Y    +  + + E+WR +R IT L +LS
Sbjct:   39 HQLGLHPHRSLHKLSKKYGPVMLLHLGSKPVIVASSVEAVRDIMKTNDLVWSNRPKSKMADRLIYGSKDVSFSPHGEYWRQIRSITVLHLLS 130
[Close]
BLAST of GR391615 vs. TrEMBL
Match: Q9ZRW6_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 3.548e-59
Identity = 102/123 (82.93%), Postives = 105/123 (85.37%), Query Frame = 1 
Query:  112 VIRLLFQSRKFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCI 480
            +IRLLFQSRKFK               HHLKRPLHRTFKGLSKTYGDIISLW+GSRLVVVVSSL EFQQCFTKNDVVL+NRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLS H I
Sbjct:   19 IIRLLFQSRKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSSLSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRI 141

HSP 2 Score: 48.1358 bits (113), Expect = 3.548e-59
Identity = 24/32 (75.00%), Postives = 24/32 (75.00%), Query Frame = 2 
Query:  470 NTASKNFPGARRDENHRLITKLAEDSSTSFAE 565
            N    NF G RRDE  RLITKLAEDSSTSFAE
Sbjct:  138 NHRINNFSGVRRDETQRLITKLAEDSSTSFAE 169
[Close]
BLAST of GR391615 vs. TrEMBL
Match: Q9XFX0_CICAR (Cytochrome P450 monooxygenase OS=Cicer arietinum GN=cyp81E3v2 PE=2 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 1.025e-58
Identity = 101/123 (82.11%), Postives = 104/123 (84.55%), Query Frame = 1 
Query:  112 VIRLLFQSRKFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCI 480
            + RLLFQSRKFK               HHLKRPLHRTFKGLSKTYGDIISLW+GSRLVVVVSSL EFQQCFTKNDVVL+NRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLS H I
Sbjct:   19 IFRLLFQSRKFKNLPPGPPSLPIIGNFHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSSLSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRI 141

HSP 2 Score: 48.1358 bits (113), Expect = 1.025e-58
Identity = 24/32 (75.00%), Postives = 24/32 (75.00%), Query Frame = 2 
Query:  470 NTASKNFPGARRDENHRLITKLAEDSSTSFAE 565
            N    NF G RRDE  RLITKLAEDSSTSFAE
Sbjct:  138 NHRINNFSGVRRDETQRLITKLAEDSSTSFAE 169
[Close]
BLAST of GR391615 vs. TrEMBL
Match: Q6WNR0_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 1.700e-52
Identity = 93/123 (75.61%), Postives = 102/123 (82.93%), Query Frame = 1 
Query:  112 VIRLLFQSRKFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCI 480
            +IRLLFQSRKFK               HHLKRPL+RTFK L++ YG++ISLW+GSRLVVVVSSL EFQ+CFTKNDVVL+NRPRFLSGKYIFYNYTTLGSTSY EHWRNLRRITSLDVLS H I
Sbjct:   19 IIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRI 141

HSP 2 Score: 40.817 bits (94), Expect = 1.700e-52
Identity = 21/32 (65.62%), Postives = 21/32 (65.62%), Query Frame = 2 
Query:  470 NTASKNFPGARRDENHRLITKLAEDSSTSFAE 565
            N    NF   RRDE  RLI KLAEDSST FAE
Sbjct:  138 NHRINNFAPIRRDETQRLIKKLAEDSSTKFAE 169
[Close]
BLAST of GR391615 vs. TrEMBL
Match: Q9ZWF2_GLYEC (Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-31 PE=2 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 6.953e-51
Identity = 88/123 (71.54%), Postives = 98/123 (79.67%), Query Frame = 1 
Query:  112 VIRLLFQSRKFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCI 480
            +  ++ ++RKFK               HHLKRPLHRTFKGLS+ YG +ISLW+GSRLVVVVSS  EFQQCFTKNDVVL+NRPRFLSGKYIFYNYTTLGSTSY EHWRNLRRIT+LDVLS H I
Sbjct:   19 IFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVISLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRI 141

HSP 2 Score: 42.3578 bits (98), Expect = 6.953e-51
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 2 
Query:  470 NTASKNFPGARRDENHRLITKLAEDSSTSFAE 565
            N    +F G RRDE  RLIT+LA+DSST+FAE
Sbjct:  138 NHRINSFSGIRRDETQRLITRLADDSSTNFAE 169
[Close]
BLAST of GR391615 vs. TrEMBL
Match: Q9MBE4_LOTJA (Cytochrome P450 OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 4.365e-47
Identity = 86/123 (69.92%), Postives = 96/123 (78.05%), Query Frame = 1 
Query:  112 VIRLLFQSRKFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCI 480
            +I+L   SRKFK               HHLKRPLHRTF+ LS+ YGD+ SLW+G+RLVVVVSS  + Q+CFTKNDVVL+NRPRFLSGKYIFYNYTTLGSTSY EHWRNLRRITSLDVLS H I
Sbjct:   19 IIKLFLGSRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALSEKYGDVFSLWFGNRLVVVVSSFADVQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRI 141

HSP 2 Score: 33.8834 bits (76), Expect = 4.365e-47
Identity = 17/32 (53.12%), Postives = 21/32 (65.62%), Query Frame = 2 
Query:  470 NTASKNFPGARRDENHRLITKLAEDSSTSFAE 565
            N    +F   RRDE  RLI KLAEDS+ +F+E
Sbjct:  138 NHRINSFSPIRRDETTRLIRKLAEDSAKNFSE 169
[Close]
BLAST of GR391615 vs. TrEMBL
Match: Q6WNQ9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 6.366e-39
Identity = 76/120 (63.33%), Postives = 88/120 (73.33%), Query Frame = 1 
Query:  115 IRLLFQSRKFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            I L   SR+ K               HHLK PLHRTF  LS+TYGDI SLW+GSRLVVVVSS     +CFTKND++L+NRPRFL+GKYIFYNYTTLGS SY +HWRNLRRIT++DVLS +
Sbjct:   18 ILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNN 137

HSP 2 Score: 28.4906 bits (62), Expect = 6.366e-39
Identity = 13/25 (52.00%), Postives = 16/25 (64.00%), Query Frame = 2 
Query:  470 NTASKNFPGARRDENHRLITKLAED 544
            N    +F G RRDE +RLI KL +D
Sbjct:  136 NNRLNSFLGVRRDETNRLIQKLLKD 160
[Close]
BLAST of GR391615 vs. TrEMBL
Match: Q2ENF7_ASTMO (Isoflavone 3'-hydroxylase (Fragment) OS=Astragalus mongholicus PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 5.334e-38
Identity = 73/94 (77.66%), Postives = 83/94 (88.30%), Query Frame = 1 
Query:  193 HHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            HHLK PLHRTF  LS+ YGDIISLW+GSRLVVVV+S    Q+CFTKNDVVL+NRP+FL+GKYIFYNYTTLGS SY +HWRNLRRIT++DVLS H
Sbjct:    4 HHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNH 97

HSP 2 Score: 24.6386 bits (52), Expect = 5.334e-38
Identity = 11/25 (44.00%), Postives = 14/25 (56.00%), Query Frame = 2 
Query:  470 NTASKNFPGARRDENHRLITKLAED 544
            N    +F G R+DE  RL+ KL  D
Sbjct:   96 NHRLNSFHGVRKDETLRLVEKLRND 120
[Close]
BLAST of GR391615 vs. TrEMBL
Match: Q6YA92_PEA (Cytochrome P450 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 2.541e-36
Identity = 75/120 (62.50%), Postives = 89/120 (74.17%), Query Frame = 1 
Query:  115 IRLLFQSRKFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            I L  QSR+ K               HHLK PLHRTF  LS+ YGDI+SLW+GSRLVVV+SS    ++CF KNDVVL+NRPRFL+GKYIFY+YTTLGS SY +HWRNLRRIT++DVLS +
Sbjct:   20 ILLQTQSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLRRITTIDVLSNN 139
[Close]
BLAST of GR391615 vs. TrEMBL
Match: Q6WNQ8_MEDTR (CYP81E8 OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 5.957e-33
Identity = 67/114 (58.77%), Postives = 80/114 (70.18%), Query Frame = 1 
Query:  133 SRKFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            SRKFK               H LK+PLH TF  LS+ YG I SLW+GSRLVVVVSSL   Q+CFTKND+VL+NRP FL+GKYI YN TT+  + Y +HWRNLRRI S+++LS H
Sbjct:   26 SRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRRILSIEILSSH 139

HSP 2 Score: 26.1794 bits (56), Expect = 5.957e-33
Identity = 14/27 (51.85%), Postives = 16/27 (59.26%), Query Frame = 2 
Query:  485 NFPGARRDENHRLITKLAEDSSTSFAE 565
            +F   RRDE  RLI KLA+ S   F E
Sbjct:  143 SFLEIRRDEIMRLIQKLAQKSYNGFTE 169
[Close]
BLAST of GR391615 vs. TrEMBL
Match: Q2LAK9_SOYBN (Cytochrome P450 monooxygenase CYP81E10 OS=Glycine max GN=CYP82E13 PE=2 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 3.554e-30
Identity = 64/118 (54.24%), Postives = 86/118 (72.88%), Query Frame = 1 
Query:  115 IRLLFQSRKFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLS 468
            ++ +FQSRK +               + L++P+HR F+ +SK YG+++SLW+GSRL VV+SS   +Q+CFTK+DV L+NR   LSGKYIFYN TT+GS S+ EHWRNLRRIT+LDVLS
Sbjct:   21 VKFVFQSRKLRNIPPGPPPLPIIGNLNLLEQPIHRFFQRMSKQYGNVVSLWFGSRLAVVISSPTAYQECFTKHDVALANRLPSLSGKYIFYNNTTVGSCSHGEHWRNLRRITALDVLS 138
[Close]
BLAST of GR391615 vs. TAIR peptide
Match: AT4G37400.1 (| Symbols: CYP81F3 | cytochrome P450, family 81, subfamily F, polypeptide 3 | chr4:17584096-17586197 FORWARD LENGTH=501)

HSP 1 Score: 115.546 bits (288), Expect = 8.355e-28
Identity = 57/97 (58.76%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:  193 HH--LKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFT-KNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            HH  LK P+HR F  LSKT+G I SL +GSR  VV+SS     QCFT +ND++LSNRP FL+ KY+ YNYTT+G+  Y +HWRNLRRI SL++LS +
Sbjct:   43 HHNLLKPPVHRLFHRLSKTHGPIFSLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWRNLRRICSLEILSSN 139

HSP 2 Score: 26.1794 bits (56), Expect = 8.355e-28
Identity = 10/20 (50.00%), Postives = 15/20 (75.00%), Query Frame = 2 
Query:  485 NFPGARRDENHRLITKLAED 544
            NF   R+DE HR++T+L+ D
Sbjct:  143 NFLHIRKDEIHRMLTRLSRD 162
[Close]
BLAST of GR391615 vs. TAIR peptide
Match: AT5G57220.1 (| Symbols: CYP81F2 | cytochrome P450, family 81, subfamily F, polypeptide 2 | chr5:23187911-23189681 FORWARD LENGTH=491)

HSP 1 Score: 118.242 bits (295), Expect = 1.843e-27
Identity = 54/95 (56.84%), Postives = 75/95 (78.95%), Query Frame = 1 
Query:  193 HHLKRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFT-KNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            H +K P+HR F+  ++ YGDI SL YGSR VVV+SSLP  ++ FT +NDV+L+NRP FL+ KY+ Y+YTT+G+ +Y +HWRNLRRI SL++LS +
Sbjct:   44 HLVKPPVHRLFRRFAEKYGDIFSLRYGSRQVVVISSLPLVRESFTGQNDVILTNRPHFLTAKYVAYDYTTIGTAAYGDHWRNLRRICSLEILSSN 138

HSP 2 Score: 22.3274 bits (46), Expect = 1.843e-27
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = 2 
Query:  488 FPGARRDENHRLITKLAED 544
            F   R+DE  RL+TKL+ +
Sbjct:  143 FLSVRKDEIRRLLTKLSRE 161
[Close]
BLAST of GR391615 vs. TAIR peptide
Match: AT2G23190.1 (| Symbols: CYP81D7 | cytochrome P450, family 81, subfamily D, polypeptide 7 | chr2:9877058-9879007 FORWARD LENGTH=543)

HSP 1 Score: 115.161 bits (287), Expect = 4.218e-26
Identity = 60/124 (48.39%), Postives = 79/124 (63.71%), Query Frame = 1 
Query:  115 IRLLFQSR--KFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGD--IISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            ++LLF  R  KF                H LK+PLHRTF   S++ GD  I SL  G+ L VVVSS    ++CFTKND+VL+NRP+F+ GK+I YN+TT+ S  Y +HWRNLRRI +L++ S H
Sbjct:   62 LKLLFGKRHSKFNLPPSPARPLPFIGHLHLLKQPLHRTFLSFSQSLGDAPIFSLRLGNHLTVVVSSYSIAEECFTKNDIVLANRPKFILGKHIEYNFTTMTSAPYGDHWRNLRRIGTLEIFSSH 185
[Close]
BLAST of GR391615 vs. TAIR peptide
Match: AT4G37320.1 (| Symbols: CYP81D5 | cytochrome P450, family 81, subfamily D, polypeptide 5 | chr4:17559742-17561690 REVERSE LENGTH=495)

HSP 1 Score: 103.99 bits (258), Expect = 1.026e-24
Identity = 49/98 (50.00%), Postives = 70/98 (71.43%), Query Frame = 1 
Query:  193 HHLKRPLHRTFKGLSKTYGD--IISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKHCI 480
            H LK+P+HRTF  +SK+ G+  I  L  G+RLV V+SS    ++CFTKNDVVL+NRP  +  K++ YN+T + + SY +HWRNLRRI ++++ S H I
Sbjct:   46 HLLKQPVHRTFHSISKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNLRRIAAVEIFSSHRI 143

HSP 2 Score: 27.335 bits (59), Expect = 1.026e-24
Identity = 14/34 (41.18%), Postives = 17/34 (50.00%), Query Frame = 2 
Query:  464 FRNTASKNFPGARRDENHRLITKLAEDSSTSFAE 565
            F +     F   R+DE  RLIT L+ DS   F E
Sbjct:  138 FSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVE 171
[Close]
BLAST of GR391615 vs. TAIR peptide
Match: AT4G37410.1 (| Symbols: CYP81F4 | cytochrome P450, family 81, subfamily F, polypeptide 4 | chr4:17590848-17592780 FORWARD LENGTH=501)

HSP 1 Score: 110.538 bits (275), Expect = 1.039e-24
Identity = 58/120 (48.33%), Postives = 76/120 (63.33%), Query Frame = 1 
Query:  118 RLLFQSRKFKXXXXXXXXXXXXXXXHHL--KRPLHRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCFT-KNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLS 468
            +  F S+K +               HHL  K P+HR F  LS  +G I  L  GSR  VV+SS    ++CFT +NDV++SNRPRFL+ KYI YNYTT+ +TSY +HWRNLRRI SL+++S
Sbjct:   18 KFFFTSKKQRYYLPPSPSYSLPILGHHLLIKPPVHRLFHRLSNIHGPIFYLRLGSRRAVVISSSSLARECFTGQNDVIVSNRPRFLTSKYIAYNYTTIATTSYGDHWRNLRRICSLEIVS 137
[Close]
BLAST of GR391615 vs. TAIR peptide
Match: AT4G37330.1 (| Symbols: CYP81D4 | cytochrome P450, family 81, subfamily D, polypeptide 4 | chr4:17562547-17564569 REVERSE LENGTH=492)

HSP 1 Score: 104.76 bits (260), Expect = 6.477e-24
Identity = 52/96 (54.17%), Postives = 68/96 (70.83%), Query Frame = 1 
Query:  193 HHLKRPLHRTFKGLSKTYGD--IISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            H LK PLHRTF  LSK+ G+  +  L  G+RLV V+SS    ++CFTKNDVVL+NRP+F   K++ YN T L S SY +HWRNLRRI ++++ S H
Sbjct:   46 HLLKPPLHRTFLSLSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNLRRIAAVEIFSTH 141

HSP 2 Score: 23.8682 bits (50), Expect = 6.477e-24
Identity = 11/22 (50.00%), Postives = 14/22 (63.64%), Query Frame = 2 
Query:  500 RRDENHRLITKLAEDSSTSFAE 565
            R+DE  RLI+ L+ DS   F E
Sbjct:  150 RKDEIRRLISHLSRDSLHGFVE 171
[Close]
BLAST of GR391615 vs. TAIR peptide
Match: AT4G37310.1 (| Symbols: CYP81H1 | cytochrome P450, family 81, subfamily H, polypeptide 1 | chr4:17556152-17558833 REVERSE LENGTH=518)

HSP 1 Score: 107.457 bits (267), Expect = 8.795e-24
Identity = 52/94 (55.32%), Postives = 70/94 (74.47%), Query Frame = 1 
Query:  193 HHLKRPL-HRTFKGLSKTYGDIISLWYGSRLVVVVSSLPEFQQCF-TKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLS 468
            H LK+PL HRT   LS + G + SL  GSRL V++SS    ++CF TKND+VL+NRPRF+ GKY+ Y+YT++ +  Y +HWRNLRRIT+L+V S
Sbjct:   45 HLLKKPLLHRTLSHLSHSLGPVFSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWRNLRRITALEVFS 138
[Close]
BLAST of GR391615 vs. TAIR peptide
Match: AT4G37340.1 (| Symbols: CYP81D3 | cytochrome P450, family 81, subfamily D, polypeptide 3 | chr4:17564953-17566706 REVERSE LENGTH=500)

HSP 1 Score: 97.8265 bits (242), Expect = 4.084e-23
Identity = 51/94 (54.26%), Postives = 67/94 (71.28%), Query Frame = 1 
Query:  199 LKRPLHRTFKGLSKTYGD--IISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            LK PLHR F  +S++ GD  IISL  G+RLV VVSS    ++CFTKNDVVL+NR   L+ K+I Y  TT+ + SY +HWRNLRRI ++++ S H
Sbjct:   47 LKPPLHRVFLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLRRIGAVEIFSAH 140

HSP 2 Score: 28.1054 bits (61), Expect = 4.084e-23
Identity = 13/27 (48.15%), Postives = 17/27 (62.96%), Query Frame = 2 
Query:  485 NFPGARRDENHRLITKLAEDSSTSFAE 565
            +F   RRDE HRLI  L+ +SS  F +
Sbjct:  144 SFSSIRRDEIHRLIACLSRNSSLEFTK 170
[Close]
BLAST of GR391615 vs. TAIR peptide
Match: AT4G37370.1 (| Symbols: CYP81D8 | cytochrome P450, family 81, subfamily D, polypeptide 8 | chr4:17569971-17571678 REVERSE LENGTH=497)

HSP 1 Score: 102.064 bits (253), Expect = 4.085e-23
Identity = 50/94 (53.19%), Postives = 68/94 (72.34%), Query Frame = 1 
Query:  199 LKRPLHRTFKGLSKTYGD--IISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            LK P+HRTF  LS++  +  I SL  G+RLV V SS    ++CFTKNDVVL+NRP F+  K++ Y+YTT+ + SY +HWRNLRRI S+++ S H
Sbjct:   47 LKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLRRIGSVEIFSNH 140

HSP 2 Score: 23.8682 bits (50), Expect = 4.085e-23
Identity = 11/34 (32.35%), Postives = 18/34 (52.94%), Query Frame = 2 
Query:  464 FRNTASKNFPGARRDENHRLITKLAEDSSTSFAE 565
            F N    +F   R+DE  RL+ +L+ + S  F +
Sbjct:  137 FSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVK 170
[Close]
BLAST of GR391615 vs. TAIR peptide
Match: AT2G23220.1 (| Symbols: CYP81D6 | cytochrome P450, family 81, subfamily D, polypeptide 6 | chr2:9884550-9886752 FORWARD LENGTH=515)

HSP 1 Score: 104.76 bits (260), Expect = 5.701e-23
Identity = 56/124 (45.16%), Postives = 73/124 (58.87%), Query Frame = 1 
Query:  115 IRLLFQSR--KFKXXXXXXXXXXXXXXXHHLKRPLHRTFKGLSKTYGD--IISLWYGSRLVVVVSSLPEFQQCFTKNDVVLSNRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSKH 474
            ++LLF  R  KF                H LK PLHRTF   S++ G   I  L  G+RL VVVSS    ++CFTKND+V +NRP  + GK+I YN TT+ S  Y +HWRNLRRI +L++ + H
Sbjct:   33 VKLLFGKRLSKFNLPPSPARPLPLIGHLHLLKLPLHRTFLSFSQSLGGAPIFCLRLGNRLTVVVSSYSIAEECFTKNDIVFANRPELILGKHIEYNSTTMTSAPYGDHWRNLRRIATLEIFASH 156
The following BLAST results are available for this feature:
BLAST of GR391615 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
C81E1_GLYEC1.306e-5170.73Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata ... [more]
C81F1_ARATH8.252e-2151.58Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CY... [more]
C81D1_ARATH5.006e-1846.94Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CY... [more]
C75A1_PINTA1.507e-1438.14Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 P... [more]
C82A1_PEA1.560e-1135.87Cytochrome P450 82A1 (Fragment) OS=Pisum sativum G... [more]
C71DB_LOTJA7.742e-1131.31Cytochrome P450 71D11 (Fragment) OS=Lotus japonicu... [more]
C71BM_ARATH7.742e-1132.99Cytochrome P450 71B22 OS=Arabidopsis thaliana GN=C... [more]
C82A2_SOYBN1.907e-932.61Cytochrome P450 82A2 OS=Glycine max GN=CYP82A2 PE=... [more]
C82A4_SOYBN3.253e-931.52Cytochrome P450 82A4 OS=Glycine max GN=CYP82A4 PE=... [more]
C71A3_SOLME3.253e-935.87Cytochrome P450 71A3 (Fragment) OS=Solanum melonge... [more]
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BLAST of GR391615 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9ZRW6_CICAR3.548e-5982.93Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2... [more]
Q9XFX0_CICAR1.025e-5882.11Cytochrome P450 monooxygenase OS=Cicer arietinum G... [more]
Q6WNR0_MEDTR1.700e-5275.61Isoflavone 2'-hydroxylase OS=Medicago truncatula P... [more]
Q9ZWF2_GLYEC6.953e-5171.54Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-... [more]
Q9MBE4_LOTJA4.365e-4769.92Cytochrome P450 OS=Lotus japonicus PE=2 SV=1[more]
Q6WNQ9_MEDTR6.366e-3963.33Isoflavone 3'-hydroxylase (Fragment) OS=Medicago t... [more]
Q2ENF7_ASTMO5.334e-3877.66Isoflavone 3'-hydroxylase (Fragment) OS=Astragalus... [more]
Q6YA92_PEA2.541e-3662.50Cytochrome P450 OS=Pisum sativum PE=2 SV=1[more]
Q6WNQ8_MEDTR5.957e-3358.77CYP81E8 OS=Medicago truncatula PE=2 SV=1[more]
Q2LAK9_SOYBN3.554e-3054.24Cytochrome P450 monooxygenase CYP81E10 OS=Glycine ... [more]
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BLAST of GR391615 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10
Match NameE-valueIdentityDescription
AT4G37400.18.355e-2858.76| Symbols: CYP81F3 | cytochrome P450, family 81, s... [more]
AT5G57220.11.843e-2756.84| Symbols: CYP81F2 | cytochrome P450, family 81, s... [more]
AT2G23190.14.218e-2648.39| Symbols: CYP81D7 | cytochrome P450, family 81, s... [more]
AT4G37320.11.026e-2450.00| Symbols: CYP81D5 | cytochrome P450, family 81, s... [more]
AT4G37410.11.039e-2448.33| Symbols: CYP81F4 | cytochrome P450, family 81, s... [more]
AT4G37330.16.477e-2454.17| Symbols: CYP81D4 | cytochrome P450, family 81, s... [more]
AT4G37310.18.795e-2455.32| Symbols: CYP81H1 | cytochrome P450, family 81, s... [more]
AT4G37340.14.084e-2354.26| Symbols: CYP81D3 | cytochrome P450, family 81, s... [more]
AT4G37370.14.085e-2353.19| Symbols: CYP81D8 | cytochrome P450, family 81, s... [more]
AT2G23220.15.701e-2345.16| Symbols: CYP81D6 | cytochrome P450, family 81, s... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450GENE3D1.10.630.10no descriptioncoord: 51..162
score: 3.4
IPR001128Cytochrome P450PANTHERPTHR19383CYTOCHROME P450coord: 25..158
score: 6.6
IPR001128Cytochrome P450PFAMPF00067p450coord: 52..157
score: 1.9
IPR001128Cytochrome P450SUPERFAMILY48264Cytochrome P450coord: 49..163
score: 1.4
NoneNo IPR availablePANTHERPTHR19383:SF43CYTOCHROME P450 SUBFAMILY 91Acoord: 25..158
score: 6.6
NoneNo IPR availableSEGsegsegcoord: 23..37
score: NAcoord: 50..64
score
NoneNo IPR availableSEGsegsegcoord: 94..101
score

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GR391615 ID=GR391615; Name=GR391615; organism=Cicer arietinum; type=EST; length=775bp
CTTATACTTATCGAGCATTTTCCGTACCCAAAGCAAAACCATTCACATTC
ACAAACCATGGGGATCTTTTCTTTGTTGTGCTACTCTCTCTTTTATCTTT
CTTTATTTTTCGTCATTAGACTTTTGTTCCAATCAAGAAAATTCAAAAAC
CTCCCACCAGGTCCACCTTCTCTTCCCATACCCGGCAACCTCCATCATCT
CAAACGTCCCCTCCACCGCACCTTTAAGGGACTCTCTAAAACTTATGGTG
ATATCATTTCTCTTTGGTATGGTTCACGTCTTGTTGTTGTTGTTTCTTCT
CTTCCCGAATTCCAACAATGCTTCACTAAAAACGATGTTGTCCTATCAAA
TAGGCCTCGTTTTCTCTCTGGAAAATACATCTTCTACAATTATACTACCC
TAGGATCCACCTCCTATAGTGAACACTGGCGCAACCTTCGTCGTATCACT
TCCCTCGATGTTCTTTCGAAACACTGCATCAAAAAACTTCCCTGGAGCCC
GAAGGGATGAGAATCACCGATTAATCACTAAGCTGGCAGAGGATTCATCC
ACTTCCTTTGCTGAATATACAACTTACCTCCAGATTCTACTAATAGCACC
CTTACACCCATCATGCGAAAGGATCTTCTGGCCCGAGATATTTACATGAA
ACGATTGTATACATTATAGGATTTTCCCAGCAACCATTTTAATTTTATGG
CACCCCGCAAAACAAATTCGTTTTAAATTATATTGGTCGAAAAAAACAAA
AACTGTTTCTCCCTCCCCATATATT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004497monooxygenase activity
GO:0005506iron ion binding
GO:0009055electron transfer activity
GO:0020037heme binding
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450