FE671090

EST Overview


Name	FE671090
Unique Name	FE671090
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	417

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FE671090 vs. SwissProt
Match: AMPN_CAUCR (Aminopeptidase N OS=Caulobacter crescentus GN=pepN PE=3 SV=2)

HSP 1 Score: 60.077 bits (144), Expect = 4.150e-9
Identity = 28/73 (38.36%), Postives = 42/73 (57.53%), Query Frame = -3

Query:  197 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSLG 415
            H   G+GY FL D ++ +D  NP  A+R+      WR Y      + +A LE+I++   LS+NV E+ SK+LG
Sbjct:  791 HDPSGAGYAFLADEILKVDAFNPMTAARLVEPLGGWRRYKPELGDLMRAQLERIVAHPNLSKNVLELASKALG 863

[Close]

BLAST of FE671090 vs. SwissProt
Match: AMPN_ECOLI (Aminopeptidase N OS=Escherichia coli (strain K12) GN=pepN PE=1 SV=2)

HSP 1 Score: 57.3806 bits (137), Expect = 2.690e-8
Identity = 30/72 (41.67%), Postives = 46/72 (63.89%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HA+DGSGY FL +++  L+  NPQVASR+     R + YD  RQ+  +A LE++     LS +++E  +K+L
Sbjct:  798 HAEDGSGYLFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLENLSGDLYEKITKAL 869

[Close]

BLAST of FE671090 vs. SwissProt
Match: AMPN_HAEIN (Aminopeptidase N OS=Haemophilus influenzae GN=pepN PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 7.827e-8
Identity = 28/72 (38.89%), Postives = 44/72 (61.11%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            H   GSGY FL D+++ L++ NPQVA+R+     R+  +D  RQ + K  LE++     LS+++FE   K+L
Sbjct:  797 HNVSGSGYRFLTDVLIRLNESNPQVAARLIEPLIRFSRFDAQRQTLMKRALERLSVVENLSKDLFEKIEKAL 868

[Close]

BLAST of FE671090 vs. TrEMBL
Match: C4WRH6_RAPSA (Putative aminopeptidase OS=Raphanus sativus PE=4 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 3.403e-25
Identity = 59/72 (81.94%), Postives = 63/72 (87.50%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLGD+VV LDKINPQVASRM SAFSRW+ YDE RQ +AKA LE IMS NGLSENVFEI SKSL
Sbjct:  813 HAKDGSGYKFLGDIVVQLDKINPQVASRMVSAFSRWKRYDETRQALAKAQLEMIMSANGLSENVFEIASKSL 884

[Close]

BLAST of FE671090 vs. TrEMBL
Match: Q9CAE1_ARATH (Putative aminopeptidase; 4537-10989 OS=Arabidopsis thaliana GN=At1g63770 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 7.580e-25
Identity = 58/72 (80.56%), Postives = 63/72 (87.50%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLGD+VV LDK+NPQVASRM SAFSRW+ YDE RQ +AKA LE IMS NGLSENVFEI SKSL
Sbjct:  891 HAKDGSGYKFLGDIVVQLDKLNPQVASRMVSAFSRWKRYDETRQGLAKAQLEMIMSANGLSENVFEIASKSL 962

[Close]

BLAST of FE671090 vs. TrEMBL
Match: Q8H0S9_ARATH (At1g63770 OS=Arabidopsis thaliana GN=At1g63770 PE=2 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 7.580e-25
Identity = 58/72 (80.56%), Postives = 63/72 (87.50%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLGD+VV LDK+NPQVASRM SAFSRW+ YDE RQ +AKA LE IMS NGLSENVFEI SKSL
Sbjct:  810 HAKDGSGYKFLGDIVVQLDKLNPQVASRMVSAFSRWKRYDETRQGLAKAQLEMIMSANGLSENVFEIASKSL 881

[Close]

BLAST of FE671090 vs. TrEMBL
Match: D7KTD6_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_893061 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 7.580e-25
Identity = 58/72 (80.56%), Postives = 63/72 (87.50%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLGD+VV LDK+NPQVASRM SAFSRW+ YDE RQ +AKA LE IMS NGLSENVFEI SKSL
Sbjct:  904 HAKDGSGYKFLGDIVVQLDKLNPQVASRMVSAFSRWKRYDETRQGLAKAQLEMIMSANGLSENVFEIASKSL 975

[Close]

BLAST of FE671090 vs. TrEMBL
Match: B9RCE0_RICCO (Aminopeptidase, putative OS=Ricinus communis GN=RCOM_1687220 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 7.580e-25
Identity = 58/72 (80.56%), Postives = 63/72 (87.50%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLG++V+ LDKINPQVASRM SAFSRWR YDE RQ +AKA LE IMS NGLSENVFEI SKSL
Sbjct:  793 HAKDGSGYQFLGEIVMQLDKINPQVASRMVSAFSRWRRYDETRQTLAKAQLEMIMSTNGLSENVFEIASKSL 864

[Close]

BLAST of FE671090 vs. TrEMBL
Match: B9NER3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_828926 PE=4 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.206e-24
Identity = 57/72 (79.17%), Postives = 63/72 (87.50%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLG++VV LDKINPQVASRM SAFSRW+ YDE RQ +AKA LE I+S NGLSENVFEI SKSL
Sbjct:  179 HAKDGSGYKFLGEIVVQLDKINPQVASRMVSAFSRWKRYDETRQNLAKAQLEMIVSANGLSENVFEIASKSL 250

[Close]

BLAST of FE671090 vs. TrEMBL
Match: B9GKF8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_548443 PE=4 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.206e-24
Identity = 57/72 (79.17%), Postives = 63/72 (87.50%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLG++VV LDKINPQVASRM SAFSRW+ YDE RQ +AKA LE I+S NGLSENVFEI SKSL
Sbjct:  845 HAKDGSGYKFLGEIVVQLDKINPQVASRMVSAFSRWKRYDETRQNLAKAQLEMIVSANGLSENVFEIASKSL 916

[Close]

BLAST of FE671090 vs. TrEMBL
Match: C5YMU8_SORBI (Putative uncharacterized protein Sb07g023850 OS=Sorghum bicolor GN=Sb07g023850 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.762e-24
Identity = 56/74 (75.68%), Postives = 64/74 (86.49%), Query Frame = -3

Query:  194 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSLGG 415
            HAKDGSGY+FLG++V+ LDKINPQVASRM SAFSRWR YD+ RQ +AKA LE I+S NGLSENV+EI SKSL G
Sbjct:  815 HAKDGSGYKFLGEIVLQLDKINPQVASRMVSAFSRWRRYDKTRQDLAKAQLEMIVSANGLSENVYEIASKSLAG 888

[Close]

BLAST of FE671090 vs. TrEMBL
Match: B9GWT2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_830842 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.762e-24
Identity = 57/72 (79.17%), Postives = 62/72 (86.11%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLG++VV LDKINPQVASRM SAFSRWR YDE RQ +AKA LE I+S NGLSENVFEI SK L
Sbjct:  877 HAKDGSGYKFLGEIVVQLDKINPQVASRMVSAFSRWRRYDETRQNLAKAQLEMIVSANGLSENVFEIASKCL 948

[Close]

BLAST of FE671090 vs. TrEMBL
Match: A9PGS2_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.762e-24
Identity = 57/72 (79.17%), Postives = 62/72 (86.11%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLG++VV LDKINPQVASRM SAFSRWR YDE RQ +AKA LE I+S NGLSENVFEI SK L
Sbjct:  408 HAKDGSGYKFLGEIVVQLDKINPQVASRMVSAFSRWRRYDETRQNLAKAQLEMIVSANGLSENVFEIASKCL 479

[Close]

BLAST of FE671090 vs. TAIR peptide
Match: AT1G63770.5 (| Symbols: | Peptidase M1 family protein | chr1:23657791-23664243 REVERSE LENGTH=1013)

HSP 1 Score: 116.701 bits (291), Expect = 4.461e-27
Identity = 58/72 (80.56%), Postives = 63/72 (87.50%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLGD+VV LDK+NPQVASRM SAFSRW+ YDE RQ +AKA LE IMS NGLSENVFEI SKSL
Sbjct:  940 HAKDGSGYKFLGDIVVQLDKLNPQVASRMVSAFSRWKRYDETRQGLAKAQLEMIMSANGLSENVFEIASKSL 1011

[Close]

BLAST of FE671090 vs. TAIR peptide
Match: AT1G63770.4 (| Symbols: | Peptidase M1 family protein | chr1:23657791-23663476 REVERSE LENGTH=895)

HSP 1 Score: 116.701 bits (291), Expect = 4.461e-27
Identity = 58/72 (80.56%), Postives = 63/72 (87.50%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLGD+VV LDK+NPQVASRM SAFSRW+ YDE RQ +AKA LE IMS NGLSENVFEI SKSL
Sbjct:  822 HAKDGSGYKFLGDIVVQLDKLNPQVASRMVSAFSRWKRYDETRQGLAKAQLEMIMSANGLSENVFEIASKSL 893

[Close]

BLAST of FE671090 vs. TAIR peptide
Match: AT1G63770.3 (| Symbols: | Peptidase M1 family protein | chr1:23657791-23664243 REVERSE LENGTH=987)

HSP 1 Score: 116.701 bits (291), Expect = 4.461e-27
Identity = 58/72 (80.56%), Postives = 63/72 (87.50%), Query Frame = -3

Query:  200 HAKDGSGYEFLGDLVVLLDKINPQVASRMGSAFSRWRGYDENRQKIAKAPLEKIMSFNGLSENVFEIGSKSL 415
            HAKDGSGY+FLGD+VV LDK+NPQVASRM SAFSRW+ YDE RQ +AKA LE IMS NGLSENVFEI SKSL
Sbjct:  914 HAKDGSGYKFLGDIVVQLDKLNPQVASRMVSAFSRWKRYDETRQGLAKAQLEMIMSANGLSENVFEIASKSL 985

[Close]

BLAST of FE671090 vs. TAIR peptide
Match: AT1G63770.2 (| Symbols: | Peptidase M1 family protein | chr1:23658165-23664243 REVERSE LENGTH=945)

HSP 1 Score: 49.2914 bits (116), Expect = 8.746e-7
Identity = 21/25 (84.00%), Postives = 24/25 (96.00%), Query Frame = -3

Query:  341 HAKDGSGYEFLGDLVVLLDKINPQV 415
            HAKDGSGY+FLGD+VV LDK+NPQV
Sbjct:  906 HAKDGSGYKFLGDIVVQLDKLNPQV 930

The following BLAST results are available for this feature:

BLAST of FE671090 vs. SwissProt
BLAST of FE671090 vs. TrEMBL
BLAST of FE671090 vs. TAIR peptide

BLAST of FE671090 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
AMPN_CAUCR	4.150e-9	38.36	Aminopeptidase N OS=Caulobacter crescentus GN=pepN...	[more]
AMPN_ECOLI	2.690e-8	41.67	Aminopeptidase N OS=Escherichia coli (strain K12) ...	[more]
AMPN_HAEIN	7.827e-8	38.89	Aminopeptidase N OS=Haemophilus influenzae GN=pepN...	[more]

BLAST of FE671090 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C4WRH6_RAPSA	3.403e-25	81.94	Putative aminopeptidase OS=Raphanus sativus PE=4 S...	[more]
Q9CAE1_ARATH	7.580e-25	80.56	Putative aminopeptidase; 4537-10989 OS=Arabidopsis...	[more]
Q8H0S9_ARATH	7.580e-25	80.56	At1g63770 OS=Arabidopsis thaliana GN=At1g63770 PE=...	[more]
D7KTD6_ARALY	7.580e-25	80.56	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9RCE0_RICCO	7.580e-25	80.56	Aminopeptidase, putative OS=Ricinus communis GN=RC...	[more]
B9NER3_POPTR	2.206e-24	79.17	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9GKF8_POPTR	2.206e-24	79.17	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C5YMU8_SORBI	3.762e-24	75.68	Putative uncharacterized protein Sb07g023850 OS=So...	[more]
B9GWT2_POPTR	3.762e-24	79.17	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PGS2_POPTR	3.762e-24	79.17	Putative uncharacterized protein OS=Populus tricho...	[more]

BLAST of FE671090 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G63770.5	4.461e-27	80.56	\| Symbols: \| Peptidase M1 family protein \| chr1:2...	[more]
AT1G63770.4	4.461e-27	80.56	\| Symbols: \| Peptidase M1 family protein \| chr1:2...	[more]
AT1G63770.3	4.461e-27	80.56	\| Symbols: \| Peptidase M1 family protein \| chr1:2...	[more]
AT1G63770.2	8.746e-7	84.00	\| Symbols: \| Peptidase M1 family protein \| chr1:2...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001930	Peptidase M1, Alanine aminopeptidase/Leukotriene A4 hydrolase	PANTHER	PTHR11533	PROTEASE M1 ZINC METALLOPROTEASE	coord: 1..38 score: 1.4
IPR012779	Peptidase M1, alanyl aminopeptidase	PANTHER	PTHR11533:SF8	AMINOPEPTIDASE	coord: 1..38 score: 1.4
None	No IPR available	GENE3D	1.25.50.10	no description	coord: 1..72 score: 7.8
None	No IPR available	PFAM	PF11940	DUF3458	coord: 1..72 score: 1.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE671090 ID=FE671090; Name=FE671090; organism=Cicer arietinum; type=EST; length=417bp
CAGCATTTTAAATTGGCTTTCAGTCACCACAATTTGAATAAATAATAATT
CTCAAAACCTGTTTTACAAAAAAACTGCCTTTGCTTTCCATGAACAGCGA
TAGGCAATTACTTTATGAAGGTTGCTCGTGTGCCCAAAGTGTTTATCCCC
TTGGCCTCAAATACACTATCTGAAAGTAGTCATTGGCACTTTAACCACCT
AAACTTTTCGAACCAATTTCAAAAACATTTTCTGATAGGCCATTAAAAGA
CATGATCTTCTCCAACGGGGCCTTTGCAATTTTTTGTCTGTTCTCATCGT
ACCCCCTCCACCTTGAAAAGGCTGACCCCATTCTAGAGGCAACCTGAGGA
TTTATTTTGTCAAGTAGAACCACAAGATCTCCCAAAAACTCGTAGCCCGA
CCCATCCTTTGCGTGGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **Molecular Function**
Term	Definition
GO:0008270	zinc ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001930	Peptidase_M1
IPR012779	Peptidase_M1_pepN

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