GR405114

EST Overview


Name	GR405114
Unique Name	GR405114
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	410

Libraries


Library Name	Type
LIBEST_024785 ICCV2 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR405114 vs. SwissProt
Match: M1330_ARATH (Uncharacterized mitochondrial protein AtMg01330 OS=Arabidopsis thaliana GN=AtMg01330 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 5.837e-11
Identity = 35/60 (58.33%), Postives = 38/60 (63.33%), Query Frame = -2

Query:   14 KVKIGGYKFPISLGAENESTFRCDTAFFILVGKERGXXXXXXXXXXXXSKAPMAREAHGK 193
            KV+IGGYKFPISLGAENEST RCDTAF  LVGKER              +A    EAH +
Sbjct:   24 KVEIGGYKFPISLGAENESTSRCDTAFSFLVGKERRSPSEPNRPMKNKRRAKPNGEAHAE 83

[Close]

BLAST of GR405114 vs. SwissProt
Match: NU2M_BETVU (NADH-ubiquinone oxidoreductase chain 2 OS=Beta vulgaris GN=ND2 PE=3 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 5.464e-9
Identity = 27/29 (93.10%), Postives = 29/29 (100.00%), Query Frame = -3

Query:  304 TVYSISLYESTITTRDEPWFGEFKLALGV 390
            +VYSISLYESTITTRDEPWFGEF+LALGV
Sbjct:  455 SVYSISLYESTITTRDEPWFGEFELALGV 483

HSP 2 Score: 52.373 bits (124), Expect = 8.723e-7
Identity = 35/74 (47.30%), Postives = 41/74 (55.41%), Query Frame = -1

Query:  222 GSSLKHQPFTQYPCTSPQSL-----------HEMNLGLVNLSWP*V*KKGLPVTARDRILRCSPPVVGKTRAGP 410
            GSSL HQP + Y  +  +S             E+ LG++          GLPVTA DRILRCSPPVVG TRAGP
Sbjct:  447 GSSLIHQP-SVYSISLYESTITTRDEPWFGEFELALGVI----------GLPVTAHDRILRCSPPVVGTTRAGP 509

[Close]

BLAST of GR405114 vs. TrEMBL
Match: Q332P4_WHEAT (Orf173 protein OS=Triticum aestivum GN=orf173 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.040e-26
Identity = 32/39 (82.05%), Postives = 32/39 (82.05%), Query Frame = 1

Query:   55 LILHWTVRMDFPVLSQLK*KRLYHIEMSIRFPPPMRWGI 171
            L LHW VRMDF VLSQ K K LYHIEMSIRFPP MRWGI
Sbjct:   93 LFLHWRVRMDFAVLSQRKWKGLYHIEMSIRFPPQMRWGI 131

HSP 2 Score: 56.9954 bits (136), Expect = 1.040e-26
Identity = 25/29 (86.21%), Postives = 27/29 (93.10%), Query Frame = 3

Query:  216 EWRPGSRLSNNRRGAPQYTIARSNWESFF 302
            E RPGSR SNNRRGAPQYTIARSNWES++
Sbjct:  144 EARPGSRRSNNRRGAPQYTIARSNWESYY 172

HSP 3 Score: 38.5058 bits (88), Expect = 1.040e-26
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 2

Query:   11 PFPMRFARHWRFALLLF 61
            PF MRFARHWRFALLLF
Sbjct:   78 PFRMRFARHWRFALLLF 94

[Close]

BLAST of GR405114 vs. TrEMBL
Match: Q8HCM5_ORYSJ (Mitchondrion orf173 OS=Oryza sativa subsp. japonica GN=orf173 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.753e-26
Identity = 32/39 (82.05%), Postives = 32/39 (82.05%), Query Frame = 1

Query:   55 LILHWTVRMDFPVLSQLK*KRLYHIEMSIRFPPPMRWGI 171
            L LHW VRMDF VLSQ K K LYHIEMSIRFPP MRWGI
Sbjct:   93 LFLHWRVRMDFAVLSQRKWKGLYHIEMSIRFPPQMRWGI 131

HSP 2 Score: 56.225 bits (134), Expect = 1.753e-26
Identity = 24/27 (88.89%), Postives = 26/27 (96.30%), Query Frame = 3

Query:  222 RPGSRLSNNRRGAPQYTIARSNWESFF 302
            RPGSR SNNRRGAPQYTIARSNWES++
Sbjct:  146 RPGSRRSNNRRGAPQYTIARSNWESYY 172

HSP 3 Score: 38.5058 bits (88), Expect = 1.753e-26
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 2

Query:   11 PFPMRFARHWRFALLLF 61
            PF MRFARHWRFALLLF
Sbjct:   78 PFRMRFARHWRFALLLF 94

[Close]

BLAST of GR405114 vs. TrEMBL
Match: Q2F956_ORYSI (Putative uncharacterized protein orf173 OS=Oryza sativa subsp. indica GN=orf173 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.753e-26
Identity = 32/39 (82.05%), Postives = 32/39 (82.05%), Query Frame = 1

Query:   55 LILHWTVRMDFPVLSQLK*KRLYHIEMSIRFPPPMRWGI 171
            L LHW VRMDF VLSQ K K LYHIEMSIRFPP MRWGI
Sbjct:   93 LFLHWRVRMDFAVLSQRKWKGLYHIEMSIRFPPQMRWGI 131

HSP 2 Score: 56.225 bits (134), Expect = 1.753e-26
Identity = 24/27 (88.89%), Postives = 26/27 (96.30%), Query Frame = 3

Query:  222 RPGSRLSNNRRGAPQYTIARSNWESFF 302
            RPGSR SNNRRGAPQYTIARSNWES++
Sbjct:  146 RPGSRRSNNRRGAPQYTIARSNWESYY 172

HSP 3 Score: 38.5058 bits (88), Expect = 1.753e-26
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 2

Query:   11 PFPMRFARHWRFALLLF 61
            PF MRFARHWRFALLLF
Sbjct:   78 PFRMRFARHWRFALLLF 94

[Close]

BLAST of GR405114 vs. TrEMBL
Match: D3DEZ2_ORYRU (Putative uncharacterized protein orf173 OS=Oryza rufipogon GN=orf173 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.753e-26
Identity = 32/39 (82.05%), Postives = 32/39 (82.05%), Query Frame = 1

Query:   55 LILHWTVRMDFPVLSQLK*KRLYHIEMSIRFPPPMRWGI 171
            L LHW VRMDF VLSQ K K LYHIEMSIRFPP MRWGI
Sbjct:   93 LFLHWRVRMDFAVLSQRKWKGLYHIEMSIRFPPQMRWGI 131

HSP 2 Score: 56.225 bits (134), Expect = 1.753e-26
Identity = 24/27 (88.89%), Postives = 26/27 (96.30%), Query Frame = 3

Query:  222 RPGSRLSNNRRGAPQYTIARSNWESFF 302
            RPGSR SNNRRGAPQYTIARSNWES++
Sbjct:  146 RPGSRRSNNRRGAPQYTIARSNWESYY 172

HSP 3 Score: 38.5058 bits (88), Expect = 1.753e-26
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 2

Query:   11 PFPMRFARHWRFALLLF 61
            PF MRFARHWRFALLLF
Sbjct:   78 PFRMRFARHWRFALLLF 94

[Close]

BLAST of GR405114 vs. TrEMBL
Match: A5AFS5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_004351 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 8.860e-20
Identity = 32/35 (91.43%), Postives = 33/35 (94.29%), Query Frame = -3

Query:  304 VEFETSTVYSISLYESTITTRDEPWFGEFKLALGV 408
            VE +TSTVYSISLYESTITTRDEPWFGE KLALGV
Sbjct:  216 VESDTSTVYSISLYESTITTRDEPWFGELKLALGV 250

HSP 2 Score: 53.1434 bits (126), Expect = 8.860e-20
Identity = 24/25 (96.00%), Postives = 24/25 (96.00%), Query Frame = -1

Query:  222 GLPVTARDRILRCSPPVVGKTRAGP 296
            GLPVTARDRILRCSPPVVG TRAGP
Sbjct:  252 GLPVTARDRILRCSPPVVGTTRAGP 276

[Close]

BLAST of GR405114 vs. TrEMBL
Match: D7SZ18_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_505.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00007203001 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.480e-19
Identity = 32/35 (91.43%), Postives = 32/35 (91.43%), Query Frame = -3

Query:  304 VEFETSTVYSISLYESTITTRDEPWFGEFKLALGV 408
            VE  TSTVYSISLYESTITTRDEPWFGE KLALGV
Sbjct:  460 VESNTSTVYSISLYESTITTRDEPWFGELKLALGV 494

HSP 2 Score: 53.1434 bits (126), Expect = 1.480e-19
Identity = 24/25 (96.00%), Postives = 24/25 (96.00%), Query Frame = -1

Query:  222 GLPVTARDRILRCSPPVVGKTRAGP 296
            GLPVTARDRILRCSPPVVG TRAGP
Sbjct:  496 GLPVTARDRILRCSPPVVGTTRAGP 520

[Close]

BLAST of GR405114 vs. TrEMBL
Match: Q9XFG1_ORYSA (Putative uncharacterized protein (Fragment) OS=Oryza sativa PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.056e-19
Identity = 32/39 (82.05%), Postives = 32/39 (82.05%), Query Frame = 1

Query:   55 LILHWTVRMDFPVLSQLK*KRLYHIEMSIRFPPPMRWGI 171
            L LHW VRMDF VLSQ K K LYHIEMSIRFPP MRWGI
Sbjct:   93 LFLHWRVRMDFAVLSQRKWKGLYHIEMSIRFPPQMRWGI 131

HSP 2 Score: 38.5058 bits (88), Expect = 5.056e-19
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 2

Query:   11 PFPMRFARHWRFALLLF 61
            PF MRFARHWRFALLLF
Sbjct:   78 PFRMRFARHWRFALLLF 94

HSP 3 Score: 30.8018 bits (68), Expect = 5.056e-19
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = 3

Query:  222 RPGSRLSNNRRGAP 263
            RPGSR SNNRRGAP
Sbjct:  146 RPGSRRSNNRRGAP 159

[Close]

BLAST of GR405114 vs. TrEMBL
Match: Q6R9K1_MAIZE (Putative uncharacterized protein orf158-a1 OS=Zea mays GN=orf158-a2 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.161e-11
Identity = 39/57 (68.42%), Postives = 40/57 (70.18%), Query Frame = 1

Query:    1 LRAPLSHALRAPLALCSPLILHWTVRMDFPVLSQLK*KRLYHIEMSIRFPPPMRWGI 171
            LRAP + A     AL   L LHW VRMDF VLSQ K K LYHIEMSIRFPP MRWGI
Sbjct:   71 LRAPSAFARHWRFALL--LFLHWRVRMDFAVLSQRKWKGLYHIEMSIRFPPQMRWGI 125

[Close]

BLAST of GR405114 vs. TrEMBL
Match: Q9MFC7_BETVU (Orf300 protein OS=Beta vulgaris subsp. vulgaris GN=orf300 PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.110e-7
Identity = 27/29 (93.10%), Postives = 29/29 (100.00%), Query Frame = -3

Query:  304 TVYSISLYESTITTRDEPWFGEFKLALGV 390
            +VYSISLYESTITTRDEPWFGEF+LALGV
Sbjct:  240 SVYSISLYESTITTRDEPWFGEFELALGV 268

[Close]

BLAST of GR405114 vs. TAIR peptide
Match: ATMG01330.1 (| Symbols: ORF107H | hypothetical protein | chrM:329082-329465 REVERSE LENGTH=127)

HSP 1 Score: 66.2402 bits (160), Expect = 6.626e-12
Identity = 35/60 (58.33%), Postives = 38/60 (63.33%), Query Frame = -2

Query:   14 KVKIGGYKFPISLGAENESTFRCDTAFFILVGKERGXXXXXXXXXXXXSKAPMAREAHGK 193
            KV+IGGYKFPISLGAENEST RCDTAF  LVGKER              +A    EAH +
Sbjct:   24 KVEIGGYKFPISLGAENESTSRCDTAFSFLVGKERRSPSEPNRPMKNKRRAKPNGEAHAE 83

The following BLAST results are available for this feature:

BLAST of GR405114 vs. SwissProt
BLAST of GR405114 vs. TrEMBL
BLAST of GR405114 vs. TAIR peptide

BLAST of GR405114 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
M1330_ARATH	5.837e-11	58.33	Uncharacterized mitochondrial protein AtMg01330 OS...	[more]
NU2M_BETVU	5.464e-9	93.10	NADH-ubiquinone oxidoreductase chain 2 OS=Beta vul...	[more]

BLAST of GR405114 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 9


Match Name	E-value	Identity	Description
Q332P4_WHEAT	1.040e-26	82.05	Orf173 protein OS=Triticum aestivum GN=orf173 PE=4...	[more]
Q8HCM5_ORYSJ	1.753e-26	82.05	Mitchondrion orf173 OS=Oryza sativa subsp. japonic...	[more]
Q2F956_ORYSI	1.753e-26	82.05	Putative uncharacterized protein orf173 OS=Oryza s...	[more]
D3DEZ2_ORYRU	1.753e-26	82.05	Putative uncharacterized protein orf173 OS=Oryza r...	[more]
A5AFS5_VITVI	8.860e-20	91.43	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7SZ18_VITVI	1.480e-19	91.43	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q9XFG1_ORYSA	5.056e-19	82.05	Putative uncharacterized protein (Fragment) OS=Ory...	[more]
Q6R9K1_MAIZE	2.161e-11	68.42	Putative uncharacterized protein orf158-a1 OS=Zea ...	[more]
Q9MFC7_BETVU	1.110e-7	93.10	Orf300 protein OS=Beta vulgaris subsp. vulgaris GN...	[more]

BLAST of GR405114 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
ATMG01330.1	6.626e-12	58.33	\| Symbols: ORF107H \| hypothetical protein \| chrM:3...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 15..28 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR405114 ID=GR405114; Name=GR405114; organism=Cicer arietinum; type=EST; length=410bp
CTCCGTGCCCCCCTTTCCCATGCGCTTCGCGCGCCATTGGCGCTTTGCTC
TCCTCTTATTCTTCATTGGACGGTTCGGATGGACTTCCCCGTTCTTTCCC
AACTAAAATAAAAAAGGCTGTATCACATCGAAATGTCGATTCGTTTTCCG
CCCCCAATGAGATGGGGAATTTGTAACCCCCTATTTTGACTTTTGTTTGG
ACCCTTCATCTTTCTGAATGGAGGCCCGGCTCGCGTCTTTCCAACAACCG
GCGGGGAGCACCTCAGTATACGATCGCGCGCAGTAACTGGGAGTCCTTTT
TTTTACACCTAAGGCCAACTTAAATTCACCAAACCAAGGTTCATCTCGTG
TAGTGATTGTGGACTCGTACAAGGATATTGAGTAAACGGTTGATGTTTCA
AACTCGACCC

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