GR402356

EST Overview


Name	GR402356
Unique Name	GR402356
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	186

Libraries


Library Name	Type
LIBEST_024785 ICCV2 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR402356 vs. TrEMBL
Match: B9RRD6_RICCO (2-oxoisovalerate dehydrogenase, putative OS=Ricinus communis GN=RCOM_1187670 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.563e-15
Identity = 33/54 (61.11%), Postives = 45/54 (83.33%), Query Frame = -3

Query:   23 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPTNISM 184
            L  IQVAE VEKPP+ADMFSD+YDVPP + C+Q KWL+E +K+HP++YP ++ +
Sbjct:  397 LNVIQVAERVEKPPVADMFSDIYDVPPPHLCDQEKWLREAIKRHPQDYPPDVPL 450

[Close]

BLAST of GR402356 vs. TrEMBL
Match: B9H495_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1077225 PE=4 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.151e-14
Identity = 34/54 (62.96%), Postives = 44/54 (81.48%), Query Frame = -3

Query:   23 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPTNISM 184
            L  IQVAE  EKPPL D+F+DVYDVPPSN  EQ KWL+ET+K HP++YP++ ++
Sbjct:  325 LDAIQVAERTEKPPLVDIFTDVYDVPPSNLSEQEKWLRETIKIHPQDYPSDFTL 378

[Close]

BLAST of GR402356 vs. TrEMBL
Match: D7U4C7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_44.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00026470001 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.160e-12
Identity = 31/52 (59.62%), Postives = 44/52 (84.62%), Query Frame = -3

Query:   29 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPTNI 184
            L  IQVAE VEKPP+A++F+DVYD PPSN CEQ K L+ET++++P++YP ++
Sbjct:  427 LDAIQVAERVEKPPIAEIFTDVYDAPPSNLCEQEKLLRETIRRYPQDYPPDV 478

[Close]

BLAST of GR402356 vs. TrEMBL
Match: B9H7L5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_559310 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.375e-12
Identity = 31/54 (57.41%), Postives = 42/54 (77.78%), Query Frame = -3

Query:   23 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPTNISM 184
            L+ IQVAE  EKPPL  +FSDVYD+PP N CEQ K L+ET+  HP++YP+++ +
Sbjct:  342 LQVIQVAEKTEKPPLKYLFSDVYDIPPPNLCEQEKQLRETIYAHPQDYPSDVPL 395

[Close]

BLAST of GR402356 vs. TrEMBL
Match: B9S2P3_RICCO (2-oxoisovalerate dehydrogenase, putative OS=Ricinus communis GN=RCOM_0559760 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.857e-11
Identity = 31/50 (62.00%), Postives = 39/50 (78.00%), Query Frame = -3

Query:   35 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPT 184
            L+ IQVAE  EKPPL DMF+DVYD+P SN  EQ K L+ET+  HP++YP+
Sbjct:  327 LQAIQVAEKTEKPPLGDMFTDVYDIPSSNLREQEKQLRETISAHPQDYPS 376

[Close]

BLAST of GR402356 vs. TrEMBL
Match: B9GTQ0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_813656 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.852e-10
Identity = 29/54 (53.70%), Postives = 42/54 (77.78%), Query Frame = -3

Query:   23 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPTNISM 184
            L+ IQVAE  EKPPL ++FSDVYD+ P N  EQ K L+ET+ +HP++YP+++ +
Sbjct:  327 LQVIQVAEKKEKPPLKELFSDVYDITPPNLREQEKQLRETIIRHPQDYPSDVPL 380

[Close]

BLAST of GR402356 vs. TrEMBL
Match: Q2QWT8_ORYSJ (2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial, putative, expressed OS=Oryza sativa subsp. japonica GN=Os12g0183100 PE=4 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 2.419e-10
Identity = 29/52 (55.77%), Postives = 41/52 (78.85%), Query Frame = -3

Query:   29 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPTNI 184
            L+ IQVAE + KPPLA++F+DVYD  PSN  EQ + L++T+KKHP +YP ++
Sbjct:  433 LKAIQVAERMPKPPLAELFTDVYDEVPSNLREQERLLRDTIKKHPADYPADV 484

[Close]

BLAST of GR402356 vs. TrEMBL
Match: Q2QWT6_ORYSJ (2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial, putative, expressed OS=Oryza sativa subsp. japonica GN=LOC_Os12g08260 PE=4 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 2.419e-10
Identity = 29/52 (55.77%), Postives = 41/52 (78.85%), Query Frame = -3

Query:   29 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPTNI 184
            L+ IQVAE + KPPLA++F+DVYD  PSN  EQ + L++T+KKHP +YP ++
Sbjct:  434 LKAIQVAERMPKPPLAELFTDVYDEVPSNLREQERLLRDTIKKHPADYPADV 485

[Close]

BLAST of GR402356 vs. TrEMBL
Match: B9GC70_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_35450 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.419e-10
Identity = 29/52 (55.77%), Postives = 41/52 (78.85%), Query Frame = -3

Query:   29 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPTNI 184
            L+ IQVAE + KPPLA++F+DVYD  PSN  EQ + L++T+KKHP +YP ++
Sbjct:  459 LKAIQVAERMPKPPLAELFTDVYDEVPSNLREQERLLRDTIKKHPADYPADV 510

[Close]

BLAST of GR402356 vs. TrEMBL
Match: B8BNH6_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_37710 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.419e-10
Identity = 29/52 (55.77%), Postives = 41/52 (78.85%), Query Frame = -3

Query:   29 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPTNI 184
            L+ IQVAE + KPPLA++F+DVYD  PSN  EQ + L++T+KKHP +YP ++
Sbjct:  426 LKAIQVAERMPKPPLAELFTDVYDEVPSNLREQERLLRDTIKKHPADYPADV 477

[Close]

BLAST of GR402356 vs. TAIR peptide
Match: AT5G09300.2 (| Symbols: | Thiamin diphosphate-binding fold (THDP-binding) superfamily protein | chr5:2884282-2886291 REVERSE LENGTH=401)

HSP 1 Score: 65.0846 bits (157), Expect = 1.018e-11
Identity = 27/54 (50.00%), Postives = 40/54 (74.07%), Query Frame = -3

Query:   23 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPTNISM 184
            L  ++VAE  EKP L +MFSDVYDVPPSN  EQ   +++T+  HP++YP+++ +
Sbjct:  348 LEALRVAEKTEKPNLQNMFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 401

[Close]

BLAST of GR402356 vs. TAIR peptide
Match: AT5G09300.1 (| Symbols: | Thiamin diphosphate-binding fold (THDP-binding) superfamily protein | chr5:2884282-2886797 REVERSE LENGTH=472)

HSP 1 Score: 65.0846 bits (157), Expect = 1.018e-11
Identity = 27/54 (50.00%), Postives = 40/54 (74.07%), Query Frame = -3

Query:   23 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYPTNISM 184
            L  ++VAE  EKP L +MFSDVYDVPPSN  EQ   +++T+  HP++YP+++ +
Sbjct:  419 LEALRVAEKTEKPNLQNMFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

[Close]

BLAST of GR402356 vs. TAIR peptide
Match: AT1G21400.1 (| Symbols: | Thiamin diphosphate-binding fold (THDP-binding) superfamily protein | chr1:7493492-7496240 FORWARD LENGTH=472)

HSP 1 Score: 58.9214 bits (141), Expect = 7.298e-10
Identity = 28/49 (57.14%), Postives = 34/49 (69.39%), Query Frame = -3

Query:   38 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREYP 184
            L+ IQ AE  EK PL ++F+DVYDV P N  EQ   LKE VKK P++YP
Sbjct:  419 LQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYP 467

[Close]

BLAST of GR402356 vs. TAIR peptide
Match: AT5G34780.1 (| Symbols: | Thiamin diphosphate-binding fold (THDP-binding) superfamily protein | chr5:12961682-12963892 REVERSE LENGTH=365)

HSP 1 Score: 53.1434 bits (126), Expect = 4.005e-8
Identity = 24/48 (50.00%), Postives = 33/48 (68.75%), Query Frame = -3

Query:   41 LRTIQVAESVEKPPLADMFSDVYDVPPSNFCEQMKWLKETVKKHPREY 184
            L+ IQ AE  EK PL ++F+DVYDV P N  E+   LKE ++K P++Y
Sbjct:  189 LQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEEELGLKELIEKQPQDY 236

The following BLAST results are available for this feature:

BLAST of GR402356 vs. TrEMBL
BLAST of GR402356 vs. TAIR peptide

BLAST of GR402356 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9RRD6_RICCO	5.563e-15	61.11	2-oxoisovalerate dehydrogenase, putative OS=Ricinu...	[more]
B9H495_POPTR	6.151e-14	62.96	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7U4C7_VITVI	1.160e-12	59.62	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9H7L5_POPTR	3.375e-12	57.41	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9S2P3_RICCO	2.857e-11	62.00	2-oxoisovalerate dehydrogenase, putative OS=Ricinu...	[more]
B9GTQ0_POPTR	1.852e-10	53.70	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q2QWT8_ORYSJ	2.419e-10	55.77	2-oxoisovalerate dehydrogenase alpha subunit, mito...	[more]
Q2QWT6_ORYSJ	2.419e-10	55.77	2-oxoisovalerate dehydrogenase alpha subunit, mito...	[more]
B9GC70_ORYSJ	2.419e-10	55.77	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8BNH6_ORYSI	2.419e-10	55.77	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GR402356 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G09300.2	1.018e-11	50.00	\| Symbols: \| Thiamin diphosphate-binding fold (TH...	[more]
AT5G09300.1	1.018e-11	50.00	\| Symbols: \| Thiamin diphosphate-binding fold (TH...	[more]
AT1G21400.1	7.298e-10	57.14	\| Symbols: \| Thiamin diphosphate-binding fold (TH...	[more]
AT5G34780.1	4.005e-8	50.00	\| Symbols: \| Thiamin diphosphate-binding fold (TH...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.970	no description	coord: 3..49 score: 0.0
None	No IPR available	PANTHER	PTHR11516	PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT (BACTERIAL AND ORGANELLAR)	coord: 1..54 score: 3.1
None	No IPR available	PANTHER	PTHR11516:SF1	2-OXOISOVALERATE DEHYDROGENASE ALPHA SUBUNIT-RELATED	coord: 1..54 score: 3.1

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR402356 ID=GR402356; Name=GR402356; organism=Cicer arietinum; type=EST; length=186bp
CTAAATATCGAGGTTAAAATTACATGGAAATATTTGTTGGGTACTCCCGT
GGATGTTTTTTTACGGTCTCCTTCAGCCATTTCATCTGTTCACAGAAATT
GGATGGAGGAACATCATAAACATCACTAAACATGTCTGCAAGTGGAGGTT
TCTCTACACTCTCTGCAACTTGAATGGTCCGCAAAA

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