FE671364

EST Overview


Name	FE671364
Unique Name	FE671364
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	629

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FE671364 vs. SwissProt
Match: IDH5_ARATH (Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial OS=Arabidopsis thaliana GN=IDH5 PE=1 SV=1)

HSP 1 Score: 273.478 bits (698), Expect = 6.381e-73
Identity = 136/174 (78.16%), Postives = 149/174 (85.63%), Query Frame = 1

Query:  148 SMASQILRRTLGSRYL--------------ANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            +MA+ + RR +G+R                + ARAF S++TPI ATLFPGDGIGPEIAESVK++F  A VPIEWEEHYVGTEIDPRTQSFLTWES+ESVRRN+VGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VDLITIRENTEGEYSGLEHQVVRG
Sbjct:    2 TMAANLARRLIGNRSTQILGAVNSSSGAASSVARAFCSSTTPITATLFPGDGIGPEIAESVKKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVRG 175

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BLAST of FE671364 vs. SwissProt
Match: LEU3_SOLTU (3-isopropylmalate dehydrogenase, chloroplastic OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 263.848 bits (673), Expect = 5.056e-70
Identity = 135/168 (80.36%), Postives = 145/168 (86.31%), Query Frame = 1

Query:  151 MASQILRRTLGSRYLANA-------RAFSSASTP--IRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            MA QI +R L  R  + A       R F+S S    IRATLFPGDGIGPEIAESV+QIF+ AEVPIEWEEHYVGTE+DPRT SFLTWES+ESVRRN+VGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+V+LITIRENTEGEYSGLEHQVVRG
Sbjct:    1 MALQIAKRLLRCRADSVASSVRFFDRTFTSESNSNLIRATLFPGDGIGPEIAESVRQIFKVAEVPIEWEEHYVGTEVDPRTNSFLTWESLESVRRNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRG 168

[Close]

BLAST of FE671364 vs. SwissProt
Match: IDH6_ARATH (Isocitrate dehydrogenase [NAD] catalytic subunit 6, mitochondrial OS=Arabidopsis thaliana GN=IDH6 PE=1 SV=2)

HSP 1 Score: 258.455 bits (659), Expect = 2.124e-68
Identity = 124/160 (77.50%), Postives = 146/160 (91.25%), Query Frame = 1

Query:  154 ASQILRRTLGSR--YLANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            +SQIL  +  S   +++ +RAF S+STPI+ATLFPGDGIGPEIAESVKQ+F AA+V I+W+E +VGTE+DPRT SFLTW++++SV +N+VGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VDLITIRENTEGEYSGLEHQVV+G
Sbjct:   16 SSQILGTSSSSSGPFISVSRAFFSSSTPIKATLFPGDGIGPEIAESVKQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWDNLQSVLKNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVKG 175

[Close]

BLAST of FE671364 vs. SwissProt
Match: IDH3A_DROME (Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Drosophila melanogaster GN=l(1)G0156 PE=2 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 8.128e-44
Identity = 87/144 (60.42%), Postives = 110/144 (76.39%), Query Frame = 1

Query:  196 ANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            A +R++SS +  +  TL PGDGIGPEI+ +V++IF AA VPIEWE   V     P  +  +   +I+SV  N++GLKGP+ TP+GKGHRSLNL LRKE NLYANVRPC SL GYKT YD+VD++TIRENTEGEYSG+EH++V G
Sbjct:   38 AASRSYSSGTKKV--TLIPGDGIGPEISAAVQKIFTAANVPIEWEAVDVTPVRGPDGKFGIPQAAIDSVNTNKIGLKGPLMTPVGKGHRSLNLALRKEFNLYANVRPCRSLEGYKTLYDDVDVVTIRENTEGEYSGIEHEIVDG 179

[Close]

BLAST of FE671364 vs. SwissProt
Match: IDHA_DICDI (Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrial OS=Dictyostelium discoideum GN=idhA PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 9.936e-42
Identity = 82/142 (57.75%), Postives = 111/142 (78.17%), Query Frame = 1

Query:  205 RAFSSASTPI-RATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            R +SS+++ I + TL PGDGIGPEI+ESVK++F A + PIEWE       +D  T   ++ E IES+ +N++GLKGP++TPIG GH+SLNL LRK  NLYAN+RPC S+PG+KTRY+NV+ + +RENTEGEYSG+E+Q V+G
Sbjct:   14 RNYSSSTSKIQKVTLIPGDGIGPEISESVKRVFSAVKAPIEWET----VVVDANTG--ISKEVIESISKNKIGLKGPISTPIGTGHQSLNLGLRKTFNLYANIRPCLSIPGHKTRYNNVNTVVVRENTEGEYSGIENQPVKG 149

[Close]

BLAST of FE671364 vs. SwissProt
Match: IDH3A_CAEEL (Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Caenorhabditis elegans GN=F43G9.1 PE=1 SV=3)

HSP 1 Score: 166.392 bits (420), Expect = 1.099e-40
Identity = 83/132 (62.88%), Postives = 102/132 (77.27%), Query Frame = 1

Query:  235 RATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSF-LTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            R TL PGDGIGPEI+ SV++IF+AA+ PI W+   V T +  R   F +    IE +  N+VGLKGP+ TPIGKGHRSLNL +RKE +LYANVRPC SL G+KT YDNVD++TIRENTEGEYSG+EH++V G
Sbjct:   26 RVTLIPGDGIGPEISASVQKIFEAADAPIAWDPVDV-TPVKGRDGVFRIPSRCIELMHANKVGLKGPLETPIGKGHRSLNLAVRKEFSLYANVRPCRSLEGHKTLYDNVDVVTIRENTEGEYSGIEHEIVPG 156

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BLAST of FE671364 vs. SwissProt
Match: IDH2_SCHPO (Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial OS=Schizosaccharomyces pombe GN=idh2 PE=1 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 2.706e-39
Identity = 79/129 (61.24%), Postives = 102/129 (79.07%), Query Frame = 1

Query:  241 TLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            T+  GDGIGPEIA+SV++IF+AA+VPIEWE   V   +   T + +  ++ ESVR+N+V LKGP+ATPIGKGH S+NLTLR+   L+ANVRPC S+ GYKT YDNV+ + IRENTEGEYSG+EH+V+ G
Sbjct:   49 TMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPILKNGTTT-IPDDAKESVRKNKVALKGPLATPIGKGHVSMNLTLRRTFGLFANVRPCVSITGYKTPYDNVNTVLIRENTEGEYSGIEHEVIPG 176

[Close]

BLAST of FE671364 vs. SwissProt
Match: IDH3A_RAT (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Rattus norvegicus GN=Idh3a PE=1 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 5.103e-38
Identity = 81/162 (50.00%), Postives = 109/162 (67.28%), Query Frame = 1

Query:  142 WSSMASQILRRTLGSRYLANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            W S  S++L     ++ +   R F+     +  TL PGDGIGPEI+ SV +IF AA+ PI+WEE  V     P  +  +  E+ ES+ +N++GLKGP+ TPI  GH S+NL LRK  +LYANVRPC S+ GYKT Y +V+++TIRENTEGEYSG+EH +V G
Sbjct:    6 WVSKVSRLLGAFHNTKQVT--RGFAGGVQTV--TLIPGDGIGPEISASVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDG 163

[Close]

BLAST of FE671364 vs. SwissProt
Match: IDH3A_MOUSE (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Mus musculus GN=Idh3a PE=1 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 5.103e-38
Identity = 81/162 (50.00%), Postives = 109/162 (67.28%), Query Frame = 1

Query:  142 WSSMASQILRRTLGSRYLANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            W S  S++L     ++ +   R F+     +  TL PGDGIGPEI+ SV +IF AA+ PI+WEE  V     P  +  +  E+ ES+ +N++GLKGP+ TPI  GH S+NL LRK  +LYANVRPC S+ GYKT Y +V+++TIRENTEGEYSG+EH +V G
Sbjct:    6 WVSKVSRLLGAFHNTKQVT--RGFAGGVQTV--TLIPGDGIGPEISASVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDG 163

[Close]

BLAST of FE671364 vs. SwissProt
Match: IDH3A_BOVIN (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Bos taurus GN=IDH3A PE=1 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 8.704e-38
Identity = 80/162 (49.38%), Postives = 108/162 (66.67%), Query Frame = 1

Query:  142 WSSMASQILRRTLGSRYLANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            W S  S++L      + +   R F+     +  TL PGDGIGPEI+ +V +IF AA+ PI+WEE  V     P  +  +  E+ ES+ +N++GLKGP+ TPI  GH S+NL LRK  +LYANVRPC S+ GYKT Y +V+++TIRENTEGEYSG+EH +V G
Sbjct:    6 WISKVSRLLGAFHNQKQVT--RGFAGGVKTV--TLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVAAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYHDVNIVTIRENTEGEYSGIEHVIVDG 163

[Close]

BLAST of FE671364 vs. TrEMBL
Match: B9MTD0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_589452 PE=3 SV=1)

HSP 1 Score: 284.648 bits (727), Expect = 3.954e-75
Identity = 139/158 (87.97%), Postives = 150/158 (94.94%), Query Frame = 1

Query:  157 SQILRRTLGSRYLANA-RAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            +Q+LRR LGS  +++A RAFSSA  PI ATLFPGDGIGPEIAE+VKQ+FQAAEVPIEWEEHYVG +IDPRTQSFLTWES+ESVRRN+VGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNV+LITIRENTEGEYSGLEHQVVRG
Sbjct:    4 TQVLRRVLGSTPISSAARAFSSAPAPIPATLFPGDGIGPEIAEAVKQVFQAAEVPIEWEEHYVGDQIDPRTQSFLTWESLESVRRNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRG 161

[Close]

BLAST of FE671364 vs. TrEMBL
Match: B9IFP1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_825258 PE=3 SV=1)

HSP 1 Score: 283.108 bits (723), Expect = 1.150e-74
Identity = 139/160 (86.88%), Postives = 148/160 (92.50%), Query Frame = 1

Query:  160 QILRRTLGS----RYLANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            Q+LRR LG+    +  + ARAFSSA  PI ATLFPGDGIGPEIAESVKQ+FQAAEVPIEWEEHYVG +IDPRTQSFLTWES+ESVRRNRVGLKGPMATP+GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNV+LITIRENTEGEYSGLEHQVVRG
Sbjct:    5 QVLRRVLGASGSTQISSTARAFSSAPAPIIATLFPGDGIGPEIAESVKQVFQAAEVPIEWEEHYVGDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPVGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRG 164

[Close]

BLAST of FE671364 vs. TrEMBL
Match: A9P904_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 282.722 bits (722), Expect = 1.502e-74
Identity = 138/160 (86.25%), Postives = 148/160 (92.50%), Query Frame = 1

Query:  160 QILRRTLGS----RYLANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            Q+LRR LG+    +  + ARAFSSA  PI ATLFPGDG+GPEIAESVKQ+FQAAEVPIEWEEHYVG +IDPRTQSFLTWES+ESVRRNRVGLKGPMATP+GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNV+LITIRENTEGEYSGLEHQVVRG
Sbjct:    5 QVLRRVLGASGSTQISSTARAFSSAPAPIIATLFPGDGVGPEIAESVKQVFQAAEVPIEWEEHYVGDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPVGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRG 164

[Close]

BLAST of FE671364 vs. TrEMBL
Match: B9S0K1_RICCO (Isocitrate dehydrogenase, putative OS=Ricinus communis GN=RCOM_1355470 PE=3 SV=1)

HSP 1 Score: 281.952 bits (720), Expect = 2.563e-74
Identity = 141/165 (85.45%), Postives = 148/165 (89.70%), Query Frame = 1

Query:  151 MASQILRRTLGSRY------LANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            MASQ+LRR  GSR        A ARA  S STPI ATLFPGDGIGPEIAESVKQ+F+ AEVPIEWEEHYVG +IDPRTQSFLTWES+ESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+V+LITIRENTEGEYSGLEHQVVRG
Sbjct:    1 MASQLLRRAFGSRSSSQIGAAAAARALFSTSTPITATLFPGDGIGPEIAESVKQVFKEAEVPIEWEEHYVGDQIDPRTQSFLTWESLESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRG 165

[Close]

BLAST of FE671364 vs. TrEMBL
Match: D7LWM4_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487101 PE=4 SV=1)

HSP 1 Score: 271.552 bits (693), Expect = 3.464e-71
Identity = 130/142 (91.55%), Postives = 137/142 (96.48%), Query Frame = 1

Query:  202 ARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            ARAF S++TPI ATLFPGDGIGPEIAESVK++F  A VPIEWEEHYVGTEIDPRTQSFLTWES+ESVRRN+VGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VDLITIRENTEGEYSGLEHQVVRG
Sbjct:   33 ARAFCSSTTPITATLFPGDGIGPEIAESVKKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVRG 174

[Close]

BLAST of FE671364 vs. TrEMBL
Match: D7TQM9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_40.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025704001 PE=4 SV=1)

HSP 1 Score: 270.396 bits (690), Expect = 7.716e-71
Identity = 137/167 (82.04%), Postives = 149/167 (89.22%), Query Frame = 1

Query:  151 MASQILRRTLGSRYLANA-----RAFSSA---STPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            M +QILRR LGS   + A     R+FSS+   S  IRATLFPGDGIGPEIAE+VKQ+F+AA+VPIEWEEHYVG +IDPRTQSFLTWES+ESVR+N VGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VDLITIRENTEGEYSGLEHQVVRG
Sbjct:    1 MGTQILRRLLGSPNSSRAFSSFNRSFSSSDSSSNLIRATLFPGDGIGPEIAEAVKQVFRAADVPIEWEEHYVGDQIDPRTQSFLTWESLESVRQNGVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVRG 167

[Close]

BLAST of FE671364 vs. TrEMBL
Match: O65852_TOBAC (Isocitrate dehydrogenase (NAD+) OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 266.159 bits (679), Expect = 1.455e-69
Identity = 134/165 (81.21%), Postives = 144/165 (87.27%), Query Frame = 1

Query:  151 MASQILRRTLGSR------YLANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            MA QI RR L SR      YL  + +  S S  IRATLFPGDGIGPEIA+SV+QIF+ AEVPIEWEEHYVG EIDPRT SFLTWES+ESVRRN+VGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+V+LITIRENTEGEYSG+EHQVVRG
Sbjct:    1 MAFQIARRLLRSRASSSIRYLDRSFSSESNSNLIRATLFPGDGIGPEIADSVRQIFKTAEVPIEWEEHYVGKEIDPRTNSFLTWESLESVRRNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGVEHQVVRG 165

[Close]

BLAST of FE671364 vs. TrEMBL
Match: D7L8C9_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_897145 PE=4 SV=1)

HSP 1 Score: 259.225 bits (661), Expect = 1.779e-67
Identity = 125/173 (72.25%), Postives = 149/173 (86.13%), Query Frame = 1

Query:  151 MASQILRRTLGSR--------------YLANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            MA+ + RR +G+R              +++ +RAF S++TPI+ATLFPGDGIGPEIAESVKQ+F AA+V I+W+E +VGTE+DPRT SFLTWE+++SV +N+VGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VDLITIRENTEGEYSGLEHQVV+G
Sbjct:    1 MAAFLARRLIGNRSSQILGTSNSSSGAFISVSRAFFSSTTPIKATLFPGDGIGPEIAESVKQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWENLQSVLKNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVKG 173

[Close]

BLAST of FE671364 vs. TrEMBL
Match: Q9SDG5_ORYSJ (NAD-dependent isocitrate dehydrogenase a OS=Oryza sativa subsp. japonica GN=P0038F12.18 PE=2 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 2.569e-66
Identity = 124/145 (85.52%), Postives = 134/145 (92.41%), Query Frame = 1

Query:  196 ANARAFSSAS-TPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            A A  FS+AS   +RATLFPGDGIGPEIAESVKQ+F  A VPIEWEEHYVGTE+DPRT+SFLTWES+ESVRRN+VGLKGPMATPIGKGHRSLNLTLRKEL LYANVRPC SLPGYKTRYD+V+L+TIRENTEGEYSGLEHQVVRG
Sbjct:   19 AAAAPFSTASGETVRATLFPGDGIGPEIAESVKQVFNVAGVPIEWEEHYVGTEVDPRTESFLTWESLESVRRNKVGLKGPMATPIGKGHRSLNLTLRKELGLYANVRPCNSLPGYKTRYDDVNLVTIRENTEGEYSGLEHQVVRG 163

[Close]

BLAST of FE671364 vs. TrEMBL
Match: B6TJM1_MAIZE (3-isopropylmalate dehydrogenase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 2.569e-66
Identity = 125/160 (78.12%), Postives = 137/160 (85.62%), Query Frame = 1

Query:  151 MASQILRRTLGSRYLANARAFSSAS-TPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            +   +L R +G         FS+ S   IRATLFPGDGIGPEIAESVKQ+F  A VPIEWEEHYVGTE+DPRT+SFLTWES+ESVRRN+VGLKGPMATPIGKGHRSLNLTLRKEL LYANVRPC SLPGYKTRYD+V+L+TIRENTEGEYSGLEHQVVRG
Sbjct:    7 LQGSVLPRIIGRDLAPAVAPFSTESGETIRATLFPGDGIGPEIAESVKQVFNVAGVPIEWEEHYVGTEVDPRTESFLTWESLESVRRNKVGLKGPMATPIGKGHRSLNLTLRKELGLYANVRPCNSLPGYKTRYDDVNLVTIRENTEGEYSGLEHQVVRG 166

[Close]

BLAST of FE671364 vs. TAIR peptide
Match: AT5G03290.1 (| Symbols: IDH-V | isocitrate dehydrogenase V | chr5:794043-795939 FORWARD LENGTH=374)

HSP 1 Score: 273.478 bits (698), Expect = 6.587e-74
Identity = 136/174 (78.16%), Postives = 149/174 (85.63%), Query Frame = 1

Query:  148 SMASQILRRTLGSRYL--------------ANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            +MA+ + RR +G+R                + ARAF S++TPI ATLFPGDGIGPEIAESVK++F  A VPIEWEEHYVGTEIDPRTQSFLTWES+ESVRRN+VGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VDLITIRENTEGEYSGLEHQVVRG
Sbjct:    2 TMAANLARRLIGNRSTQILGAVNSSSGAASSVARAFCSSTTPITATLFPGDGIGPEIAESVKKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVRG 175

[Close]

BLAST of FE671364 vs. TAIR peptide
Match: AT3G09810.1 (| Symbols: IDH-VI | isocitrate dehydrogenase VI | chr3:3008753-3011070 FORWARD LENGTH=374)

HSP 1 Score: 258.455 bits (659), Expect = 2.193e-69
Identity = 124/160 (77.50%), Postives = 146/160 (91.25%), Query Frame = 1

Query:  154 ASQILRRTLGSR--YLANARAFSSASTPIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            +SQIL  +  S   +++ +RAF S+STPI+ATLFPGDGIGPEIAESVKQ+F AA+V I+W+E +VGTE+DPRT SFLTW++++SV +N+VGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VDLITIRENTEGEYSGLEHQVV+G
Sbjct:   16 SSQILGTSSSSSGPFISVSRAFFSSSTPIKATLFPGDGIGPEIAESVKQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWDNLQSVLKNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVKG 175

[Close]

BLAST of FE671364 vs. TAIR peptide
Match: AT4G35260.1 (| Symbols: IDH1, IDH-I | isocitrate dehydrogenase 1 | chr4:16774494-16776233 REVERSE LENGTH=367)

HSP 1 Score: 134.035 bits (336), Expect = 6.237e-32
Identity = 67/133 (50.38%), Postives = 96/133 (72.18%), Query Frame = 1

Query:  229 PIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            P   TL PGDGIGP +  +V+Q+ +A   PI +E++ V  E+     S +  E +ES+R+N+V LKG + TP+G G  SLN+ LRKEL+L+A++  C++LPG  TR++NVD++ IRENTEGEY+GLEH+VV G
Sbjct:   37 PRAVTLIPGDGIGPLVTNAVEQVMEAMHAPIFFEKYDVHGEM-----SRVPPEVMESIRKNKVCLKGGLKTPVGGGVSSLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPG 164

[Close]

BLAST of FE671364 vs. TAIR peptide
Match: AT4G35650.1 (| Symbols: IDH-III | isocitrate dehydrogenase III | chr4:16908584-16909973 FORWARD LENGTH=368)

HSP 1 Score: 131.724 bits (330), Expect = 3.095e-31
Identity = 66/133 (49.62%), Postives = 93/133 (69.92%), Query Frame = 1

Query:  229 PIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            P   TL PGDGIGP +  +V+Q+ +A   P+ +E + V   +    +     E IESV+RN+V LKG +ATP+G G  SLN+ LRKEL+++A++  C ++PG  TR++NVD++ IRENTEGEYSGLEH+VV G
Sbjct:   38 PRTVTLIPGDGIGPLVTGAVEQVMEAMHAPVHFERYEVLGNMRKVPE-----EVIESVKRNKVCLKGGLATPVGGGVSSLNMQLRKELDIFASLVNCINVPGLVTRHENVDIVVIRENTEGEYSGLEHEVVPG 165

[Close]

BLAST of FE671364 vs. TAIR peptide
Match: AT2G17130.2 (| Symbols: IDH2, IDH-II | isocitrate dehydrogenase subunit 2 | chr2:7461062-7462466 REVERSE LENGTH=363)

HSP 1 Score: 128.257 bits (321), Expect = 3.422e-30
Identity = 61/133 (45.86%), Postives = 92/133 (69.17%), Query Frame = 1

Query:  229 PIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            P   TL PGDG+GP +  +V+Q+ +A   P+ +E   V  ++    +  L     ES+++N+V LKG + TP+G G  SLN+ LRKEL+L+A++  C++LPG  +R++NVD++ IRENTEGEY+GLEH+VV G
Sbjct:   37 PRPVTLIPGDGVGPLVTNAVQQVMEAMHAPVYFEPFEVHGDMKSLPEGLL-----ESIKKNKVCLKGGLKTPVGGGVSSLNVNLRKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPG 164

[Close]

BLAST of FE671364 vs. TAIR peptide
Match: AT2G17130.1 (| Symbols: IDH2, IDH-II | isocitrate dehydrogenase subunit 2 | chr2:7461062-7462466 REVERSE LENGTH=367)

HSP 1 Score: 128.257 bits (321), Expect = 3.422e-30
Identity = 61/133 (45.86%), Postives = 92/133 (69.17%), Query Frame = 1

Query:  229 PIRATLFPGDGIGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            P   TL PGDG+GP +  +V+Q+ +A   P+ +E   V  ++    +  L     ES+++N+V LKG + TP+G G  SLN+ LRKEL+L+A++  C++LPG  +R++NVD++ IRENTEGEY+GLEH+VV G
Sbjct:   37 PRPVTLIPGDGVGPLVTNAVQQVMEAMHAPVYFEPFEVHGDMKSLPEGLL-----ESIKKNKVCLKGGLKTPVGGGVSSLNVNLRKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPG 164

[Close]

BLAST of FE671364 vs. TAIR peptide
Match: AT1G32480.1 (| Symbols: IDH-IV | isocitrate dehydrogenase IV | chr1:11741083-11741727 FORWARD LENGTH=214)

HSP 1 Score: 90.8929 bits (224), Expect = 6.055e-19
Identity = 46/122 (37.70%), Postives = 75/122 (61.48%), Query Frame = 1

Query:  262 IGPEIAESVKQIFQAAEVPIEWEEHYVGTEIDPRTQSFLTWESIESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRG 627
            I   +  +V Q+  A + P+ +E +     I  +  + LTWE ++S+R+N+V L G +   +  G        RKEL+L+A++  C++L G  +R++NVD++ IRENTEGEY+G EH+VV G
Sbjct:    8 IDSNVTNAVHQVMDAMQAPVYFETYI----IKGKNMNHLTWEVVDSIRKNKVCLNGRVNNSLCGG-------ARKELDLFASLVDCFNLNGQPSRHENVDIVVIRENTEGEYAGREHEVVPG 118

The following BLAST results are available for this feature:

BLAST of FE671364 vs. SwissProt
BLAST of FE671364 vs. TrEMBL
BLAST of FE671364 vs. TAIR peptide

BLAST of FE671364 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
IDH5_ARATH	6.381e-73	78.16	Isocitrate dehydrogenase [NAD] catalytic subunit 5...	[more]
LEU3_SOLTU	5.056e-70	80.36	3-isopropylmalate dehydrogenase, chloroplastic OS=...	[more]
IDH6_ARATH	2.124e-68	77.50	Isocitrate dehydrogenase [NAD] catalytic subunit 6...	[more]
IDH3A_DROME	8.128e-44	60.42	Probable isocitrate dehydrogenase [NAD] subunit al...	[more]
IDHA_DICDI	9.936e-42	57.75	Isocitrate dehydrogenase [NAD] regulatory subunit ...	[more]
IDH3A_CAEEL	1.099e-40	62.88	Probable isocitrate dehydrogenase [NAD] subunit al...	[more]
IDH2_SCHPO	2.706e-39	61.24	Isocitrate dehydrogenase [NAD] subunit 2, mitochon...	[more]
IDH3A_RAT	5.103e-38	50.00	Isocitrate dehydrogenase [NAD] subunit alpha, mito...	[more]
IDH3A_MOUSE	5.103e-38	50.00	Isocitrate dehydrogenase [NAD] subunit alpha, mito...	[more]
IDH3A_BOVIN	8.704e-38	49.38	Isocitrate dehydrogenase [NAD] subunit alpha, mito...	[more]

BLAST of FE671364 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9MTD0_POPTR	3.954e-75	87.97	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9IFP1_POPTR	1.150e-74	86.88	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9P904_POPTR	1.502e-74	86.25	Putative uncharacterized protein OS=Populus tricho...	[more]
B9S0K1_RICCO	2.563e-74	85.45	Isocitrate dehydrogenase, putative OS=Ricinus comm...	[more]
D7LWM4_ARALY	3.464e-71	91.55	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7TQM9_VITVI	7.716e-71	82.04	Whole genome shotgun sequence of line PN40024, sca...	[more]
O65852_TOBAC	1.455e-69	81.21	Isocitrate dehydrogenase (NAD+) OS=Nicotiana tabac...	[more]
D7L8C9_ARALY	1.779e-67	72.25	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9SDG5_ORYSJ	2.569e-66	85.52	NAD-dependent isocitrate dehydrogenase a OS=Oryza ...	[more]
B6TJM1_MAIZE	2.569e-66	78.13	3-isopropylmalate dehydrogenase OS=Zea mays PE=2 S...	[more]

BLAST of FE671364 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT5G03290.1	6.587e-74	78.16	\| Symbols: IDH-V \| isocitrate dehydrogenase V \| ch...	[more]
AT3G09810.1	2.193e-69	77.50	\| Symbols: IDH-VI \| isocitrate dehydrogenase VI \| ...	[more]
AT4G35260.1	6.237e-32	50.38	\| Symbols: IDH1, IDH-I \| isocitrate dehydrogenase ...	[more]
AT4G35650.1	3.095e-31	49.62	\| Symbols: IDH-III \| isocitrate dehydrogenase III ...	[more]
AT2G17130.2	3.422e-30	45.86	\| Symbols: IDH2, IDH-II \| isocitrate dehydrogenase...	[more]
AT2G17130.1	3.422e-30	45.86	\| Symbols: IDH2, IDH-II \| isocitrate dehydrogenase...	[more]
AT1G32480.1	6.055e-19	37.70	\| Symbols: IDH-IV \| isocitrate dehydrogenase IV \| ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001804	Isocitrate/isopropylmalate dehydrogenase	GENE3D	3.40.718.10	no description	coord: 34..166 score: 7.8
IPR001804	Isocitrate/isopropylmalate dehydrogenase	PANTHER	PTHR11835	DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE	coord: 50..166 score: 3.2
IPR001804	Isocitrate/isopropylmalate dehydrogenase	PFAM	PF00180	Iso_dh	coord: 37..162 score: 2.1
None	No IPR available	PANTHER	PTHR11835:SF5	ISOCITRATE DEHYDROGENASE [NAD]	coord: 50..166 score: 3.2
None	No IPR available	SUPERFAMILY	53659	Isocitrate/Isopropylmalate dehydrogenase-like	coord: 27..164 score: 2.1
None	No IPR available	SEG	seg	seg	coord: 12..28 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE671364 ID=FE671364; Name=FE671364; organism=Cicer arietinum; type=EST; length=629bp
ATTCGCTTATGCGCACTCCGCGTCAGAATCTTCAACCCTAAACTAAACTC
AACTCAGCCATCGAACCCCCAACTTTGATTGGGCAGCGTCCACGTAAACT
CCGCATCGATCTTCACAGCTTCCAGAATATAAAAGGGAGTGTGGTCAAGT
ATGGCTTCTCAGATCCTGAGACGAACCCTCGGAAGTCGCTATCTGGCAAA
CGCTAGGGCCTTCTCTTCTGCTTCCACTCCCATTCGGGCCACTCTTTTCC
CCGGAGATGGTATTGGTCCTGAGATTGCTGAATCCGTCAAACAGATATTC
CAAGCTGCTGAAGTTCCAATTGAATGGGAAGAGCATTATGTAGGGACTGA
AATTGACCCTAGAACACAGAGCTTTCTTACATGGGAAAGTATAGAATCAG
TGAGGAGAAATAGGGTTGGCTTGAAAGGGCCAATGGCCACTCCTATTGGA
AAAGGGCACCGATCATTAAACCTTACCCTGAGAAAAGAACTTAATTTATA
TGCCAATGTCCGACCTTGTTACAGCCTTCCTGGCTACAAAACTCGATATG
ATAATGTAGATCTCATCACAATCCGTGAAAATACAGAAGGCGAGTACAGT
GGACTTGAACACCAGGTCGTGAGGGGTGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000287	magnesium ion binding
GO:0051287	NAD binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR001804	Isocitrate/isopropylmalate_DH

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