FE671619

EST Overview


Name	FE671619
Unique Name	FE671619
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	593

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FE671619 vs. SwissProt
Match: GLYC1_DICDI (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum GN=shmt1 PE=1 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 4.262e-28
Identity = 58/82 (70.73%), Postives = 69/82 (84.15%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L E D+E+  ++N+EKDRQF+ LELIASENFTSRAVMEA+GS  TNKY+EG PG RYYGG E +DELETLCQ+RAL AF +D
Sbjct:   10 LKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSRYYGGTEVVDELETLCQKRALKAFRLD 91

[Close]

BLAST of FE671619 vs. SwissProt
Match: GLYC_HUMAN (Serine hydroxymethyltransferase, cytosolic OS=Homo sapiens GN=SHMT1 PE=1 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 8.038e-27
Identity = 58/82 (70.73%), Postives = 68/82 (82.93%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L ++D EV+ II KE +RQ   LELIASENF SRAV+EA+GSCL NKYSEG PG+RYYGG EFIDELETLCQ+RAL A+ +D
Sbjct:   26 LKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELETLCQKRALQAYKLD 107

[Close]

BLAST of FE671619 vs. SwissProt
Match: GLYC_MOUSE (Serine hydroxymethyltransferase, cytosolic OS=Mus musculus GN=Shmt1 PE=1 SV=2)

HSP 1 Score: 119.398 bits (298), Expect = 1.371e-26
Identity = 58/86 (67.44%), Postives = 70/86 (81.40%), Query Frame = 2

Query:  329 LDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L   L ++D+EV++II KE +RQ   LELIASENF SRAV+EA+GS L NKYSEG PG+RYYGG EFIDELE LCQ+RAL A+H+D
Sbjct:   16 LSQPLKDSDAEVYSIIKKESNRQRVGLELIASENFASRAVLEALGSSLNNKYSEGYPGQRYYGGTEFIDELEMLCQKRALQAYHLD 101

[Close]

BLAST of FE671619 vs. SwissProt
Match: GLYC_RABIT (Serine hydroxymethyltransferase, cytosolic OS=Oryctolagus cuniculus GN=SHMT1 PE=1 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 3.989e-26
Identity = 57/82 (69.51%), Postives = 68/82 (82.93%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L ++D+EV+ II KE +RQ   LELIASENF SRAV+EA+GSCL NKYSEG PG+RYYGG E IDELETLCQ+RAL A+ +D
Sbjct:   26 LKDSDAEVYDIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEHIDELETLCQKRALQAYGLD 107

[Close]

BLAST of FE671619 vs. SwissProt
Match: GLYC2_DICDI (Serine hydroxymethyltransferase 2 OS=Dictyostelium discoideum GN=shmt2 PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 3.989e-26
Identity = 53/82 (64.63%), Postives = 69/82 (84.15%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            ++E+D E++ ++ KEK RQF  LELIASENFTSRAVME++GSC TNKY+EGLPG RYYGGNE +D+LE LC +RAL  F+++
Sbjct:   34 VSESDPEIYDLMMKEKQRQFTGLELIASENFTSRAVMESIGSCFTNKYAEGLPGARYYGGNEVVDQLENLCIKRALETFNLN 115

[Close]

BLAST of FE671619 vs. SwissProt
Match: GLYC_PONAB (Serine hydroxymethyltransferase, cytosolic OS=Pongo abelii GN=SHMT1 PE=2 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 6.804e-26
Identity = 56/82 (68.29%), Postives = 67/82 (81.71%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L ++D EV+ II KE +RQ   LEL ASENF S+AV+EA+GSCL NKYSEG PG+RYYGG EFIDELETLCQ+RAL A+ +D
Sbjct:   26 LKDSDVEVYNIIKKESNRQRVGLELFASENFASQAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELETLCQKRALQAYKLD 107

[Close]

BLAST of FE671619 vs. SwissProt
Match: GLYM_HUMAN (Serine hydroxymethyltransferase, mitochondrial OS=Homo sapiens GN=SHMT2 PE=1 SV=3)

HSP 1 Score: 115.931 bits (289), Expect = 1.516e-25
Identity = 54/82 (65.85%), Postives = 66/82 (80.49%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L+++D E+  ++ +EKDRQ R LELIASENF SRA +EA+GSCL NKYSEG PGKRYYGG E +DE+E LCQ RAL AF +D
Sbjct:   49 LSDSDPEMWELLQREKDRQCRGLELIASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDLD 130

[Close]

BLAST of FE671619 vs. SwissProt
Match: GLYM_BOVIN (Serine hydroxymethyltransferase, mitochondrial OS=Bos taurus GN=SHMT2 PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.516e-25
Identity = 54/82 (65.85%), Postives = 66/82 (80.49%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L+++D E+  ++ +EKDRQ R LELIASENF SRA +EA+GSCL NKYSEG PGKRYYGG E +DE+E LCQ RAL AF +D
Sbjct:   49 LSDSDPEMWELLRREKDRQCRGLELIASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDLD 130

[Close]

BLAST of FE671619 vs. SwissProt
Match: GLYC_SCHPO (Probable serine hydroxymethyltransferase, cytosolic OS=Schizosaccharomyces pombe GN=shm2 PE=2 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 1.980e-25
Identity = 56/83 (67.47%), Postives = 64/83 (77.11%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVDG 589
            L E D  V+ I+  EK RQ  S+ LIASENFTSRAVM+A+GS + NKYSEG PG RYYGGNEFID+ E LCQ RAL AFH+DG
Sbjct:   18 LAECDPTVYKILESEKSRQKESIALIASENFTSRAVMDALGSIMQNKYSEGYPGARYYGGNEFIDQAERLCQTRALEAFHLDG 100

[Close]

BLAST of FE671619 vs. SwissProt
Match: GLYC_SHEEP (Serine hydroxymethyltransferase, cytosolic OS=Ovis aries GN=SHMT1 PE=1 SV=3)

HSP 1 Score: 115.161 bits (287), Expect = 2.586e-25
Identity = 56/82 (68.29%), Postives = 65/82 (79.27%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L + D EV+ II KE +RQ   LELIASENF SRAV+EA+GSCL NKYSEG PG+RYYGG EFIDELE LCQ+RAL  + +D
Sbjct:   26 LKDNDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELEVLCQKRALQVYGLD 107

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BLAST of FE671619 vs. TrEMBL
Match: C6ZJY9_SOYBN (Serine hydroxymethyltransferase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 225.713 bits (574), Expect = 1.882e-57
Identity = 118/166 (71.08%), Postives = 132/166 (79.52%), Query Frame = 2

Query:  122 MSSCTGVAMMSSLQQPLWTKLHNFTSNKGHTSHFFLP---------CKASHVEASLVAGNPSSLSFSVPQIGGDGCSFLDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVDGN 592
            M +CT   M S  QQP+WTK  NF +    +++ FLP          KASHVEASLV G PSS+ FSVP+IGGDG SFLDYGL+EAD +V  II+KEKDRQF+SLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNE+IDELE LCQ+RALAAFHVD N
Sbjct:    1 MQACT--TMSSLQQQPIWTKGLNFPAKGYGSNNGFLPQVRFCNIKPSKASHVEASLVTGKPSSVPFSVPEIGGDGSSFLDYGLSEADPDVRAIIDKEKDRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDELEILCQQRALAAFHVDEN 164

[Close]

BLAST of FE671619 vs. TrEMBL
Match: D7STM0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_8.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00035348001 PE=4 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 1.306e-50
Identity = 107/165 (64.85%), Postives = 124/165 (75.15%), Query Frame = 2

Query:  122 MSSCTGVAMMSSLQQPLWTKLHNFTSNKGHTSHF--------FLPCKASHVEASLVAGNP-SSLSFSVPQIGGDGCSFLDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVDG 589
            M +C+G A M SLQQP+W K  +F  N    + F          PC+ S+++ SLV G P SS+S +VP  G  G +F+D+GLNEAD EV  II KEK RQ +SLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQ+RALAAFH+DG
Sbjct:    1 MQACSGTAAMGSLQQPIWIKGSSFAPNGSTVNGFPHQIKLNSVKPCR-SYIKGSLVTGKPPSSVSVTVPGNGDSGVNFIDHGLNEADPEVCAIIYKEKQRQMKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQKRALAAFHLDG 164

[Close]

BLAST of FE671619 vs. TrEMBL
Match: B9S1D7_RICCO (Serine hydroxymethyltransferase OS=Ricinus communis GN=RCOM_1657030 PE=3 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 2.910e-50
Identity = 107/163 (65.64%), Postives = 121/163 (74.23%), Query Frame = 2

Query:  122 MSSCTGVAMMSSLQQPLWTKLHNFTSNKGHTSHF--------FLPCKASHVEASLVAGNPSSLSFSVPQIGGDGCSFLDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            M  CTG  +M SLQQP+W K  +    + +   F        F PC+  +++ SLVAG P S S SVP+I GD  SF DYGL+EAD EV  II KEK+RQF+SLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNE IDELETLCQERALAAF +D
Sbjct:    1 MQGCTGAQVMGSLQQPVWIKGSSLPLKQSNFVGFPHQIKLNSFRPCRYLNIQGSLVAGRPPS-SVSVPEIEGDRSSFKDYGLSEADPEVREIIEKEKNRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQERALAAFGLD 162

[Close]

BLAST of FE671619 vs. TrEMBL
Match: B9HDQ7_POPTR (Serine hydroxymethyltransferase OS=Populus trichocarpa GN=SHMT8 PE=3 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 4.202e-49
Identity = 106/165 (64.24%), Postives = 123/165 (74.55%), Query Frame = 2

Query:  122 MSSCTGVAMMSSLQQPLWTKLHNFTSNKGHTSHF--------FLPCKASHVEASLVAGNP-SSLSFSVPQIGGDGCSFLDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVDG 589
            M + +G A+M SLQQP+ +K   F   +     F          PC+AS +E SLV G P SS+S  +P+ G D  SF DYGL EAD EV  IINKEKDRQF+SLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNE+IDELETLCQ+RALA+F++DG
Sbjct:    1 MQATSGAAVMGSLQQPVLSKGPAFPMKRSVIIGFPYQVKLNSVKPCRASSLEGSLVTGRPPSSVSVPIPETGADISSFKDYGLGEADPEVLEIINKEKDRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDELETLCQKRALASFNLDG 165

[Close]

BLAST of FE671619 vs. TrEMBL
Match: A9PL11_POPTM (Serine hydroxymethyltransferase OS=Populus tremuloides GN=SHMT8 PE=2 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 1.597e-48
Identity = 105/165 (63.64%), Postives = 123/165 (74.55%), Query Frame = 2

Query:  122 MSSCTGVAMMSSLQQPLWTKLHNFTSNKGHTSHF--------FLPCKASHVEASLVAGNP-SSLSFSVPQIGGDGCSFLDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVDG 589
            M + +G A+M SLQQP+ +K   F   +     F          PC+AS +E SLV G P SS+S  +P+ G D  SF DYGL+EAD EV  II KEKDRQF+SLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNE+IDELETLCQ+RALA+F++DG
Sbjct:    1 MQATSGAAVMGSLQQPVLSKGPAFPMKRSVIIGFPYQVKLNSVKPCRASSLEGSLVTGRPPSSVSVPIPETGADISSFKDYGLSEADPEVLEIIKKEKDRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDELETLCQKRALASFNLDG 165

[Close]

BLAST of FE671619 vs. TrEMBL
Match: D7MA38_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_491471 PE=4 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 1.226e-40
Identity = 96/166 (57.83%), Postives = 112/166 (67.47%), Query Frame = 2

Query:  122 MSSCTGVAMMSSLQQP----------LWTKLHNFTSNKGHTSHFFLPCKASHVEASLV-AGNPSSLSFSVPQIGGDGCSFLDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            M +C G   M+SLQQP          L   +  F+       +F  P ++S +E +LV     SS+S    ++     +F DYGL E D EV TII KEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNE+ID+LETLCQ RALAAF +D
Sbjct:    1 MQACCGGTSMASLQQPGRVQGSVFPPLMPPVTKFSQQLKF--NFSKPFRSSFLERNLVFERRASSVSLPNVEMSSKDIAFADYGLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLD 164

[Close]

BLAST of FE671619 vs. TrEMBL
Match: Q94JQ3_ARATH (Serine hydroxymethyltransferase OS=Arabidopsis thaliana GN=At4g32520 PE=2 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 3.019e-39
Identity = 95/164 (57.93%), Postives = 110/164 (67.07%), Query Frame = 2

Query:  122 MSSCTGVAMMSSLQQPLWTKLHNFTSNKGHTSHFFL--------PCKASHVEASLVAG-NPSSLSFSVPQIGGDGCSFLDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            M +C G   M+SLQQP   +   F       + F          P ++S ++ +LV+    SS+S    +I      F DYGL E D EV TII KEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNE+ID+LETLCQ RALAAF +D
Sbjct:    1 MQACCGGNSMASLQQPGRVQGSVFPPIMPPVTKFSQQLKFNISKPFRSSFLKRNLVSEMRASSVSLPNVEISSKEIPFEDYGLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLD 164

[Close]

BLAST of FE671619 vs. TrEMBL
Match: Q9SUU0_ARATH (Serine hydroxymethyltransferase OS=Arabidopsis thaliana GN=F8B4.220 PE=2 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 1.831e-36
Identity = 77/92 (83.70%), Postives = 80/92 (86.96%), Query Frame = 2

Query:  311 GDGCSFLDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            G    F DYGL E D EV TII KEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNE+ID+LETLCQ RALAAF +D
Sbjct:    6 GKEIPFEDYGLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLD 97

[Close]

BLAST of FE671619 vs. TrEMBL
Match: D5A8K3_PICSI (Serine hydroxymethyltransferase OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 3.233e-33
Identity = 75/122 (61.48%), Postives = 87/122 (71.31%), Query Frame = 2

Query:  248 VEASLVAGNPSSLSFSVPQIGGDGCSF-------LDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVDGN 592
            + +SL  G PS      P +    C+F       +D  L+E D E+H II +EK RQFR LELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYY GNEFID+ E LCQ+RAL AFH+D +
Sbjct:   55 IRSSLTMGKPS------PSVPAQPCNFPEGKQNFVDLPLSELDPEMHAIIEQEKQRQFRGLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYAGNEFIDQSERLCQKRALEAFHLDSS 170

[Close]

BLAST of FE671619 vs. TrEMBL
Match: A9T736_PHYPA (Serine hydroxymethyltransferase OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_168438 PE=3 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 3.574e-32
Identity = 74/109 (67.89%), Postives = 83/109 (76.15%), Query Frame = 2

Query:  260 LVAGNPSSLSFSVPQIGGDGCSFLDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            LV    ++   SVP+       F+D  L+E D +VH II  EK RQFR LELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNE+ID+ E LCQ+RAL AFHVD
Sbjct:   63 LVVCASAAAPVSVPE---GATRFVDPPLSEIDPDVHAIIECEKRRQFRGLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDQSERLCQQRALTAFHVD 168

[Close]

BLAST of FE671619 vs. TAIR peptide
Match: AT4G32520.2 (| Symbols: SHM3 | serine hydroxymethyltransferase 3 | chr4:15689642-15692334 REVERSE LENGTH=529)

HSP 1 Score: 165.236 bits (417), Expect = 2.288e-41
Identity = 95/164 (57.93%), Postives = 110/164 (67.07%), Query Frame = 2

Query:  122 MSSCTGVAMMSSLQQPLWTKLHNFTSNKGHTSHFFL--------PCKASHVEASLVAG-NPSSLSFSVPQIGGDGCSFLDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            M +C G   M+SLQQP   +   F       + F          P ++S ++ +LV+    SS+S    +I      F DYGL E D EV TII KEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNE+ID+LETLCQ RALAAF +D
Sbjct:    1 MQACCGGNSMASLQQPGRVQGSVFPPIMPPVTKFSQQLKFNISKPFRSSFLKRNLVSEMRASSVSLPNVEISSKEIPFEDYGLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLD 164

[Close]

BLAST of FE671619 vs. TAIR peptide
Match: AT4G32520.1 (| Symbols: SHM3, AtSHMT3 | serine hydroxymethyltransferase 3 | chr4:15689642-15692334 REVERSE LENGTH=529)

HSP 1 Score: 165.236 bits (417), Expect = 2.288e-41
Identity = 95/164 (57.93%), Postives = 110/164 (67.07%), Query Frame = 2

Query:  122 MSSCTGVAMMSSLQQPLWTKLHNFTSNKGHTSHFFL--------PCKASHVEASLVAG-NPSSLSFSVPQIGGDGCSFLDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            M +C G   M+SLQQP   +   F       + F          P ++S ++ +LV+    SS+S    +I      F DYGL E D EV TII KEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNE+ID+LETLCQ RALAAF +D
Sbjct:    1 MQACCGGNSMASLQQPGRVQGSVFPPIMPPVTKFSQQLKFNISKPFRSSFLKRNLVSEMRASSVSLPNVEISSKEIPFEDYGLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLD 164

[Close]

BLAST of FE671619 vs. TAIR peptide
Match: AT4G13930.1 (| Symbols: SHM4 | serine hydroxymethyltransferase 4 | chr4:8048013-8050021 REVERSE LENGTH=471)

HSP 1 Score: 121.709 bits (304), Expect = 2.901e-28
Identity = 58/82 (70.73%), Postives = 66/82 (80.49%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L   D E+H +I KEK RQ R +ELIASENFTS AV+EA+GS LTNKYSEG+PG RYYGGNEFIDE+E LC+ RAL AFH D
Sbjct:   12 LVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGIPGNRYYGGNEFIDEIENLCRSRALEAFHCD 93

[Close]

BLAST of FE671619 vs. TAIR peptide
Match: AT1G22020.1 (| Symbols: SHM6 | serine hydroxymethyltransferase 6 | chr1:7754599-7757087 FORWARD LENGTH=599)

HSP 1 Score: 120.168 bits (300), Expect = 8.439e-28
Identity = 56/79 (70.89%), Postives = 66/79 (83.54%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAF 577
            + EAD E+H  + KEK RQFR +ELIASENF  RAVMEA+GS LTNKYSEG+PG RYY GN++ID++E LCQERALAAF
Sbjct:  142 IEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQYIDQIEILCQERALAAF 220

[Close]

BLAST of FE671619 vs. TAIR peptide
Match: AT4G13890.1 (| Symbols: SHM5, EDA36, EDA37 | Pyridoxal phosphate (PLP)-dependent transferases superfamily protein | chr4:8032146-8033718 REVERSE LENGTH=470)

HSP 1 Score: 119.013 bits (297), Expect = 1.880e-27
Identity = 55/80 (68.75%), Postives = 67/80 (83.75%), Query Frame = 2

Query:  341 LNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFH 580
            L+  D E++ +I KEK RQ R +ELIA+ENFTS AVMEA+GSCLTNKYSEG+PG RYYGG EFIDE+E+LC+ R+L AFH
Sbjct:   12 LDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGMPGNRYYGGTEFIDEIESLCRSRSLEAFH 91

[Close]

BLAST of FE671619 vs. TAIR peptide
Match: AT4G37930.1 (| Symbols: SHM1, STM, SHMT1 | serine transhydroxymethyltransferase 1 | chr4:17831891-17834742 REVERSE LENGTH=517)

HSP 1 Score: 112.849 bits (281), Expect = 1.347e-25
Identity = 56/86 (65.12%), Postives = 66/86 (76.74%), Query Frame = 2

Query:  329 LDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L+  L E D E+  II  EK RQ++ LELI SENFTS +VM+AVGS +TNKYSEG PG RYYGGNE+ID  ETLCQ+RAL AF +D
Sbjct:   51 LNAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLD 136

[Close]

BLAST of FE671619 vs. TAIR peptide
Match: AT5G26780.2 (| Symbols: SHM2 | serine hydroxymethyltransferase 2 | chr5:9418299-9421725 FORWARD LENGTH=533)

HSP 1 Score: 112.464 bits (280), Expect = 1.760e-25
Identity = 56/86 (65.12%), Postives = 66/86 (76.74%), Query Frame = 2

Query:  329 LDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L+  L+E D EV  II  EK RQ++  ELI SENFTS +VM+AVGS +TNKYSEG PG RYYGGNE+ID  ETLCQ+RAL AF +D
Sbjct:   51 LNASLDEIDPEVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFQLD 136

[Close]

BLAST of FE671619 vs. TAIR peptide
Match: AT5G26780.3 (| Symbols: SHM2 | serine hydroxymethyltransferase 2 | chr5:9418299-9421725 FORWARD LENGTH=533)

HSP 1 Score: 112.464 bits (280), Expect = 1.760e-25
Identity = 56/86 (65.12%), Postives = 66/86 (76.74%), Query Frame = 2

Query:  329 LDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L+  L+E D EV  II  EK RQ++  ELI SENFTS +VM+AVGS +TNKYSEG PG RYYGGNE+ID  ETLCQ+RAL AF +D
Sbjct:   51 LNASLDEIDPEVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFQLD 136

[Close]

BLAST of FE671619 vs. TAIR peptide
Match: AT5G26780.1 (| Symbols: SHM2 | serine hydroxymethyltransferase 2 | chr5:9418299-9421725 FORWARD LENGTH=517)

HSP 1 Score: 112.464 bits (280), Expect = 1.760e-25
Identity = 56/86 (65.12%), Postives = 66/86 (76.74%), Query Frame = 2

Query:  329 LDYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVD 586
            L+  L+E D EV  II  EK RQ++  ELI SENFTS +VM+AVGS +TNKYSEG PG RYYGGNE+ID  ETLCQ+RAL AF +D
Sbjct:   51 LNASLDEIDPEVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFQLD 136

[Close]

BLAST of FE671619 vs. TAIR peptide
Match: AT1G36370.1 (| Symbols: SHM7 | serine hydroxymethyltransferase 7 | chr1:13696240-13698576 REVERSE LENGTH=598)

HSP 1 Score: 112.079 bits (279), Expect = 2.298e-25
Identity = 52/87 (59.77%), Postives = 68/87 (78.16%), Query Frame = 2

Query:  332 DYGLNEADSEVHTIINKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEFIDELETLCQERALAAFHVDGN 592
            D  ++ AD ++H ++ KEK RQ R +ELIASENF  RAVMEA+GS LTNKYSEG+PG RYY GN++ID++E LC ERAL AF ++ +
Sbjct:  135 DQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESD 221

The following BLAST results are available for this feature:

BLAST of FE671619 vs. SwissProt
BLAST of FE671619 vs. TrEMBL
BLAST of FE671619 vs. TAIR peptide

BLAST of FE671619 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GLYC1_DICDI	4.262e-28	70.73	Serine hydroxymethyltransferase 1 OS=Dictyostelium...	[more]
GLYC_HUMAN	8.038e-27	70.73	Serine hydroxymethyltransferase, cytosolic OS=Homo...	[more]
GLYC_MOUSE	1.371e-26	67.44	Serine hydroxymethyltransferase, cytosolic OS=Mus ...	[more]
GLYC_RABIT	3.989e-26	69.51	Serine hydroxymethyltransferase, cytosolic OS=Oryc...	[more]
GLYC2_DICDI	3.989e-26	64.63	Serine hydroxymethyltransferase 2 OS=Dictyostelium...	[more]
GLYC_PONAB	6.804e-26	68.29	Serine hydroxymethyltransferase, cytosolic OS=Pong...	[more]
GLYM_HUMAN	1.516e-25	65.85	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]
GLYM_BOVIN	1.516e-25	65.85	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]
GLYC_SCHPO	1.980e-25	67.47	Probable serine hydroxymethyltransferase, cytosoli...	[more]
GLYC_SHEEP	2.586e-25	68.29	Serine hydroxymethyltransferase, cytosolic OS=Ovis...	[more]

BLAST of FE671619 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6ZJY9_SOYBN	1.882e-57	71.08	Serine hydroxymethyltransferase OS=Glycine max PE=...	[more]
D7STM0_VITVI	1.306e-50	64.85	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9S1D7_RICCO	2.910e-50	65.64	Serine hydroxymethyltransferase OS=Ricinus communi...	[more]
B9HDQ7_POPTR	4.202e-49	64.24	Serine hydroxymethyltransferase OS=Populus trichoc...	[more]
A9PL11_POPTM	1.597e-48	63.64	Serine hydroxymethyltransferase OS=Populus tremulo...	[more]
D7MA38_ARALY	1.226e-40	57.83	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q94JQ3_ARATH	3.019e-39	57.93	Serine hydroxymethyltransferase OS=Arabidopsis tha...	[more]
Q9SUU0_ARATH	1.831e-36	83.70	Serine hydroxymethyltransferase OS=Arabidopsis tha...	[more]
D5A8K3_PICSI	3.233e-33	61.48	Serine hydroxymethyltransferase OS=Picea sitchensi...	[more]
A9T736_PHYPA	3.574e-32	67.89	Serine hydroxymethyltransferase OS=Physcomitrella ...	[more]

BLAST of FE671619 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G32520.2	2.288e-41	57.93	\| Symbols: SHM3 \| serine hydroxymethyltransferase ...	[more]
AT4G32520.1	2.288e-41	57.93	\| Symbols: SHM3, AtSHMT3 \| serine hydroxymethyltra...	[more]
AT4G13930.1	2.901e-28	70.73	\| Symbols: SHM4 \| serine hydroxymethyltransferase ...	[more]
AT1G22020.1	8.439e-28	70.89	\| Symbols: SHM6 \| serine hydroxymethyltransferase ...	[more]
AT4G13890.1	1.880e-27	68.75	\| Symbols: SHM5, EDA36, EDA37 \| Pyridoxal phosphat...	[more]
AT4G37930.1	1.347e-25	65.12	\| Symbols: SHM1, STM, SHMT1 \| serine transhydroxym...	[more]
AT5G26780.2	1.760e-25	65.12	\| Symbols: SHM2 \| serine hydroxymethyltransferase ...	[more]
AT5G26780.3	1.760e-25	65.12	\| Symbols: SHM2 \| serine hydroxymethyltransferase ...	[more]
AT5G26780.1	1.760e-25	65.12	\| Symbols: SHM2 \| serine hydroxymethyltransferase ...	[more]
AT1G36370.1	2.298e-25	59.77	\| Symbols: SHM7 \| serine hydroxymethyltransferase ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 14..29 score
IPR001085	Serine hydroxymethyltransferase	PANTHER	PTHR11680	SERINE HYDROXYMETHYLTRANSFERASE	coord: 79..158 score: 3.5
IPR001085	Serine hydroxymethyltransferase	PFAM	PF00464	SHMT	coord: 77..158 score: 3.5
IPR015421	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	GENE3D	3.40.640.10	no description	coord: 106..158 score: 4.1
IPR015424	Pyridoxal phosphate-dependent transferase, major domain	SUPERFAMILY	53383	PLP-dependent transferases	coord: 70..158 score: 2.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE671619 ID=FE671619; Name=FE671619; organism=Cicer arietinum; type=EST; length=593bp
ATTCGAATTCGCAGCAGCCAGTCTTTCCCAAAAACCCATTTCTTTCTCTC
TTCTCTTCTCTTCACTTACCTTTTCCTCTTCTTCACTGTGTGTGGTGAGC
GCAAAACCCTAATTTATCATCATGTCGTCGTGTACTGGAGTTGCAATGAT
GAGTTCTTTGCAACAACCTCTCTGGACTAAGTTGCATAATTTCACTTCTA
ATAAGGGGCATACCAGTCATTTCTTTTTACCCTGCAAAGCTTCTCATGTC
GAAGCAAGTTTAGTCGCTGGAAACCCATCATCTTTGTCTTTCTCTGTCCC
TCAAATTGGAGGTGATGGATGTAGCTTCTTGGACTATGGCTTGAATGAAG
CTGATTCTGAAGTTCATACAATAATTAACAAGGAAAAGGACCGTCAATTT
AGAAGCCTTGAACTTATTGCTTCTGAGAATTTTACATCTAGAGCAGTGAT
GGAAGCAGTTGGTTCATGCCTCACAAACAAGTACTCAGAAGGTTTGCCTG
GTAAAAGGTATTATGGTGGCAATGAGTTCATTGATGAGCTTGAAACCCTA
TGTCAAGAAAGGGCACTGGCTGCATTTCATGTTGATGGTAACA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004372	glycine hydroxymethyltransferase activity
GO:0003824	catalytic activity
GO:0030170	pyridoxal phosphate binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006544	glycine metabolic process
GO:0006563	L-serine metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001085	Ser_HO-MeTrfase
IPR015421	PyrdxlP-dep_Trfase_major
IPR015424	PyrdxlP-dep_Trfase

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