FE672862

EST Overview


Name	FE672862
Unique Name	FE672862
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	299

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FE672862 vs. TrEMBL
Match: D7T1S3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_264.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00005197001 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.780e-11
Identity = 40/72 (55.56%), Postives = 45/72 (62.50%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVEXXXXXXXXXXXXXXYLGLKNT 219
            KVA CFL    P   ISRGF FVTME+VEDA+RC+ +L QSVLEGR + VE              YLGLKNT
Sbjct:  116 KVASCFLVM-EPRTRISRGFAFVTMETVEDANRCIKHLNQSVLEGRYITVEKSRRKRARTPTPGHYLGLKNT 186

[Close]

BLAST of FE672862 vs. TrEMBL
Match: C6T0S6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.631e-11
Identity = 39/72 (54.17%), Postives = 43/72 (59.72%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVEXXXXXXXXXXXXXXYLGLKNT 219
            KV+ CFL    P   ISRGF FVTMES EDA RC+ YL QSVLEGR + +E              YLGLKNT
Sbjct:   96 KVSSCFLVV-EPRTRISRGFAFVTMESAEDAERCIKYLNQSVLEGRYITIERSRRKRARTPTPGHYLGLKNT 166

[Close]

BLAST of FE672862 vs. TrEMBL
Match: C6TAB9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.089e-11
Identity = 38/72 (52.78%), Postives = 45/72 (62.50%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVEXXXXXXXXXXXXXXYLGLKNT 219
            KVA CFL    P   ISRGF F+TM++VEDA+RC+ YL QSVLEGR + VE              YLGLK+T
Sbjct:   94 KVASCFLVV-EPRTRISRGFAFITMDTVEDANRCIKYLNQSVLEGRYITVERSRRKRPRTPTPGHYLGLKST 164

[Close]

BLAST of FE672862 vs. TrEMBL
Match: B9SXC4_RICCO (Arginine/serine-rich-splicing factor, putative OS=Ricinus communis GN=RCOM_0783220 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.802e-10
Identity = 39/71 (54.93%), Postives = 44/71 (61.97%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVEXXXXXXXXXXXXXXYLGLKN 216
            KVA CFL    P   ISRGF FVTM++VEDA+RCV YL QSVLEGR + VE              YLGLK+
Sbjct:   99 KVASCFLVV-EPRTRISRGFAFVTMDNVEDANRCVKYLNQSVLEGRYITVEKSRRKRPRTPTPGHYLGLKS 168

[Close]

BLAST of FE672862 vs. TrEMBL
Match: Q494N5_ARATH (At4g35785 OS=Arabidopsis thaliana GN=At4g35785 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.526e-9
Identity = 36/72 (50.00%), Postives = 43/72 (59.72%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVEXXXXXXXXXXXXXXYLGLKNT 219
            KVA CFL    P   +SRGF FVTM S++DA RC+ YL QSVLEGR + VE              YLGLK++
Sbjct:   97 KVASCFLVM-EPRTRVSRGFAFVTMSSLKDAERCIKYLNQSVLEGRYITVERSRRKRPRTPTPGHYLGLKSS 167

[Close]

BLAST of FE672862 vs. TrEMBL
Match: Q3E9Q6_ARATH (Putative uncharacterized protein At4g35785.1 OS=Arabidopsis thaliana GN=At4g35785 PE=4 SV=2)

HSP 1 Score: 65.855 bits (159), Expect = 1.526e-9
Identity = 36/72 (50.00%), Postives = 43/72 (59.72%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVEXXXXXXXXXXXXXXYLGLKNT 219
            KVA CFL    P   +SRGF FVTM S++DA RC+ YL QSVLEGR + VE              YLGLK++
Sbjct:   96 KVASCFLVM-EPRTRVSRGFAFVTMSSLKDAERCIKYLNQSVLEGRYITVERSRRKRPRTPTPGHYLGLKSS 166

[Close]

BLAST of FE672862 vs. TrEMBL
Match: D5A9M6_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.602e-9
Identity = 32/61 (52.46%), Postives = 38/61 (62.30%), Query Frame = 1

Query:   37 PLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVEXXXXXXXXXXXXXXYLGLKNT 219
            P   ISRGFGFVTM S+EDA RC+ YL QS+LEGR + VE              YLG+K+T
Sbjct:   77 PRTRISRGFGFVTMGSLEDAERCIKYLNQSILEGRFITVEKSRRKRPRTPTPGNYLGVKST 137

[Close]

BLAST of FE672862 vs. TrEMBL
Match: P93396_TOBAC (Transformer-SR ribonucleoprotein (Fragment) OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 4.331e-9
Identity = 31/60 (51.67%), Postives = 38/60 (63.33%), Query Frame = 1

Query:   37 PLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVEXXXXXXXXXXXXXXYLGLKN 216
            P   ISRGF F+TM+S+EDA+RC+ +L QSVLEGR + VE              YLGLKN
Sbjct:  101 PRSRISRGFAFITMDSLEDANRCIKHLNQSVLEGRYITVEKSRRKRARTPTPGHYLGLKN 160

HSP 2 Score: 20.4014 bits (41), Expect = 4.331e-9
Identity = 8/12 (66.67%), Postives = 11/12 (91.67%), Query Frame = 2

Query:    5 RLLSVFLMVEPR 40
            ++ SVFL+VEPR
Sbjct:   91 KVKSVFLVVEPR 102

[Close]

BLAST of FE672862 vs. TrEMBL
Match: C6T424_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 9.889e-9
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVE 156
            KVA CFL    P   ISRGF F+TM++VEDA+RC+ YL QSVLEGR + VE
Sbjct:   94 KVASCFLVV-EPRTRISRGFAFITMDTVEDANRCIKYLNQSVLEGRYITVE 143

[Close]

BLAST of FE672862 vs. TrEMBL
Match: D7MC38_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_912788 PE=4 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.687e-8
Identity = 32/51 (62.75%), Postives = 37/51 (72.55%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVE 156
            KVA CFL    P   +SRGF FVTM+SV+DA RC+ YL QSVLEGR + VE
Sbjct:   78 KVASCFLVM-EPRTRVSRGFAFVTMDSVKDAERCIKYLNQSVLEGRYITVE 127

[Close]

BLAST of FE672862 vs. TAIR peptide
Match: AT4G35785.4 (| Symbols: | RNA-binding (RRM/RBD/RNP motifs) family protein | chr4:16953404-16955127 REVERSE LENGTH=201)

HSP 1 Score: 60.4622 bits (145), Expect = 4.667e-12
Identity = 31/51 (60.78%), Postives = 36/51 (70.59%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVE 156
            KVA CFL    P   +SRGF FVTM S++DA RC+ YL QSVLEGR + VE
Sbjct:  111 KVASCFLVM-EPRTRVSRGFAFVTMSSLKDAERCIKYLNQSVLEGRYITVE 160

HSP 2 Score: 25.7942 bits (55), Expect = 4.667e-12
Identity = 12/22 (54.55%), Postives = 14/22 (63.64%), Query Frame = 3

Query:  159 VTEKATKNSKIWALSWAEKYQR 224
            + EKA +NS    LSW EK QR
Sbjct:  174 IQEKAPENSHPRPLSWLEKLQR 195

[Close]

BLAST of FE672862 vs. TAIR peptide
Match: AT4G35785.2 (| Symbols: | RNA-binding (RRM/RBD/RNP motifs) family protein | chr4:16953211-16955127 REVERSE LENGTH=239)

HSP 1 Score: 65.855 bits (159), Expect = 6.015e-12
Identity = 36/72 (50.00%), Postives = 43/72 (59.72%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVEXXXXXXXXXXXXXXYLGLKNT 219
            KVA CFL    P   +SRGF FVTM S++DA RC+ YL QSVLEGR + VE              YLGLK++
Sbjct:   97 KVASCFLVM-EPRTRVSRGFAFVTMSSLKDAERCIKYLNQSVLEGRYITVERSRRKRPRTPTPGHYLGLKSS 167

[Close]

BLAST of FE672862 vs. TAIR peptide
Match: AT4G35785.1 (| Symbols: | RNA-binding (RRM/RBD/RNP motifs) family protein | chr4:16953211-16955127 REVERSE LENGTH=238)

HSP 1 Score: 65.855 bits (159), Expect = 6.015e-12
Identity = 36/72 (50.00%), Postives = 43/72 (59.72%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVEXXXXXXXXXXXXXXYLGLKNT 219
            KVA CFL    P   +SRGF FVTM S++DA RC+ YL QSVLEGR + VE              YLGLK++
Sbjct:   96 KVASCFLVM-EPRTRVSRGFAFVTMSSLKDAERCIKYLNQSVLEGRYITVERSRRKRPRTPTPGHYLGLKSS 166

[Close]

BLAST of FE672862 vs. TAIR peptide
Match: AT4G35785.5 (| Symbols: | RNA-binding (RRM/RBD/RNP motifs) family protein | chr4:16953404-16955127 REVERSE LENGTH=244)

HSP 1 Score: 60.4622 bits (145), Expect = 2.527e-10
Identity = 31/51 (60.78%), Postives = 36/51 (70.59%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVE 156
            KVA CFL    P   +SRGF FVTM S++DA RC+ YL QSVLEGR + VE
Sbjct:  111 KVASCFLVM-EPRTRVSRGFAFVTMSSLKDAERCIKYLNQSVLEGRYITVE 160

[Close]

BLAST of FE672862 vs. TAIR peptide
Match: AT4G35785.3 (| Symbols: | RNA-binding (RRM/RBD/RNP motifs) family protein | chr4:16953708-16955127 REVERSE LENGTH=207)

HSP 1 Score: 60.4622 bits (145), Expect = 2.527e-10
Identity = 31/51 (60.78%), Postives = 36/51 (70.59%), Query Frame = 1

Query:    4 KVAFCFLDGGAPLRAISRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVE 156
            KVA CFL    P   +SRGF FVTM S++DA RC+ YL QSVLEGR + VE
Sbjct:   97 KVASCFLVM-EPRTRVSRGFAFVTMSSLKDAERCIKYLNQSVLEGRYITVE 146

[Close]

BLAST of FE672862 vs. TAIR peptide
Match: AT1G07350.1 (| Symbols: | RNA-binding (RRM/RBD/RNP motifs) family protein | chr1:2257732-2260101 REVERSE LENGTH=382)

HSP 1 Score: 51.6026 bits (122), Expect = 1.174e-7
Identity = 25/54 (46.30%), Postives = 33/54 (61.11%), Query Frame = 1

Query:   52 SRGFGFVTMESVEDASRCVSYLGQSVLEGRCVNVEXXXXXXXXXXXXXXYLGLK 213
            SRGFGF++M+SV DA+RC+  L  SVL+GR + VE              YLGL+
Sbjct:  115 SRGFGFISMKSVGDANRCIRSLDHSVLQGRVITVEKARRRRGRTPTPGKYLGLR 168

The following BLAST results are available for this feature:

BLAST of FE672862 vs. TrEMBL
BLAST of FE672862 vs. TAIR peptide

BLAST of FE672862 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7T1S3_VITVI	2.780e-11	55.56	Whole genome shotgun sequence of line PN40024, sca...	[more]
C6T0S6_SOYBN	3.631e-11	54.17	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TAB9_SOYBN	8.089e-11	52.78	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9SXC4_RICCO	1.802e-10	54.93	Arginine/serine-rich-splicing factor, putative OS=...	[more]
Q494N5_ARATH	1.526e-9	50.00	At4g35785 OS=Arabidopsis thaliana GN=At4g35785 PE=...	[more]
Q3E9Q6_ARATH	1.526e-9	50.00	Putative uncharacterized protein At4g35785.1 OS=Ar...	[more]
D5A9M6_PICSI	2.602e-9	52.46	Putative uncharacterized protein OS=Picea sitchens...	[more]
P93396_TOBAC	4.331e-9	51.67	Transformer-SR ribonucleoprotein (Fragment) OS=Nic...	[more]
C6T424_SOYBN	9.889e-9	62.75	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7MC38_ARALY	1.687e-8	62.75	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of FE672862 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT4G35785.4	4.667e-12	60.78	\| Symbols: \| RNA-binding (RRM/RBD/RNP motifs) fam...	[more]
AT4G35785.2	6.015e-12	50.00	\| Symbols: \| RNA-binding (RRM/RBD/RNP motifs) fam...	[more]
AT4G35785.1	6.015e-12	50.00	\| Symbols: \| RNA-binding (RRM/RBD/RNP motifs) fam...	[more]
AT4G35785.5	2.527e-10	60.78	\| Symbols: \| RNA-binding (RRM/RBD/RNP motifs) fam...	[more]
AT4G35785.3	2.527e-10	60.78	\| Symbols: \| RNA-binding (RRM/RBD/RNP motifs) fam...	[more]
AT1G07350.1	1.174e-7	46.30	\| Symbols: \| RNA-binding (RRM/RBD/RNP motifs) fam...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 18..49 score: 0.0
IPR012677	Nucleotide-binding, alpha-beta plait	GENE3D	3.30.70.330	no description	coord: 14..65 score: 9.8
None	No IPR available	PANTHER	PTHR15241	TRANSFORMER-2-RELATED	coord: 17..99 score: 9.4
None	No IPR available	SEG	seg	seg	coord: 53..66 score: NAcoord: 74..98 score
None	No IPR available	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 18..88 score: 1.7

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE672862 ID=FE672862; Name=FE672862; organism=Cicer arietinum; type=EST; length=299bp
CGAAAGGTTGCTTTCTGTTTTCTTGATGGTGGAGCCCCGTTACGCGCTAT
TTCTCGTGGTTTTGGGTTTGTTACTATGGAATCTGTTGAGGATGCCAGCC
GCTGTGTCTCGTACCTCGGGCAATCTGTTTTAGAAGGTCGCTGTGTTAAT
GTAGAGCGGTCACGGAGAAAGCGACCAAGAACTCGAAAATCTGGGCGTTA
TCTTGGGCTGAAAAATACCAGAGATGATGGTCACCGTGGTGACCGGGGAA
GGCATAGGGGTGGACATGGTCGTGATGATTATCACTATCGGAGGTCTCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0000166	nucleotide binding

Vocabulary: **INTERPRO**
Term	Definition
IPR000504	RRM_dom
IPR012677	Nucleotide-bd_a/b_plait_sf

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