GR912371

EST Overview


Name	GR912371
Unique Name	GR912371
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	460

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR912371 vs. SwissProt
Match: SYQ_LUPLU (Glutaminyl-tRNA synthetase OS=Lupinus luteus PE=2 SV=2)

HSP 1 Score: 258.455 bits (659), Expect = 6.582e-69
Identity = 127/138 (92.03%), Postives = 130/138 (94.20%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EFETRRASYYWLLHALGIYQPYVWEYSRLN+SN VMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGM  TAIN+FVRG+GITRSDGTLISV RLEYHVREELNKTAPR MVVL PLKVVITNLEA SAIEVDAK
Sbjct:  469 TLEFETRRASYYWLLHALGIYQPYVWEYSRLNVSNTVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMTPTAINAFVRGMGITRSDGTLISVERLEYHVREELNKTAPRAMVVLHPLKVVITNLEAKSAIEVDAK 606

HSP 2 Score: 22.7126 bits (47), Expect = 6.582e-69
Identity = 8/8 (100.00%), Postives = 8/8 (100.00%), Query Frame = 3

Query:  435 KKWPDAQA 458
            KKWPDAQA
Sbjct:  606 KKWPDAQA 613

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BLAST of GR912371 vs. SwissProt
Match: SYQ_DICDI (Probable glutaminyl-tRNA synthetase OS=Dictyostelium discoideum GN=glnS PE=2 SV=2)

HSP 1 Score: 144.05 bits (362), Expect = 2.826e-34
Identity = 71/139 (51.08%), Postives = 94/139 (67.63%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAKN 441
            T EFE RR +Y WL+  LG+Y+P VWEY+RLN+++ V+SKRK+  LV  K V+GWDDPRL TL   RR+G    AIN     IG+TR++GT IS   LE   R++L+  A R M V DP+KVVITN   + + E++A N
Sbjct:  459 TLEFEIRRLTYNWLIDVLGLYRPVVWEYARLNLTHTVLSKRKIITLVQNKIVNGWDDPRLSTLNAFRRKGYTPEAINLLCDTIGVTRTNGTTISYELLELCCRQDLDGKATRAMAVFDPIKVVITNYPEDKSEEINAPN 597

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BLAST of GR912371 vs. SwissProt
Match: SYQ_DROME (Probable glutaminyl-tRNA synthetase OS=Drosophila melanogaster GN=Aats-gln PE=1 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 5.330e-33
Identity = 70/136 (51.47%), Postives = 96/136 (70.59%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVD 432
            T EF++RR+SYYWL +ALGIY P  WEY RLN++ A++SKRK+ +L+TE+ V  WDDPRL TL  LRRRG  + AIN+F   +G+T +    +  A LE  VR+ LN TAPR +VVL+PLKV I N    + ++++
Sbjct:  460 TKEFQSRRSSYYWLCNALGIYCPVQWEYGRLNMNYALVSKRKIAKLITEQIVHDWDDPRLFTLTALRRRGFPAEAINNFCAQMGVTGAQ-IAVDPAMLEAAVRDVLNVTAPRRLVVLEPLKVTIKNFPHAAPVQLE 594

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BLAST of GR912371 vs. SwissProt
Match: SYQ_BOVIN (Glutaminyl-tRNA synthetase OS=Bos taurus GN=QARS PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 5.893e-32
Identity = 71/139 (51.08%), Postives = 91/139 (65.47%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAKN 441
            T EF+ RR+SY+WL +AL +Y P  WEY RLN+  AV+SKRK+ +LV    V  WDDPRL TL  LRRRG    AIN+F   +G+T +  T +    LE  VR+ LN TAPR M VL+PL+VVITN  A  A+++   N
Sbjct:  457 TKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVAAGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQ-TTMEPHLLEACVRDVLNDTAPRAMAVLEPLQVVITNFPATKALDIQVPN 594

[Close]

BLAST of GR912371 vs. SwissProt
Match: SYQ_EDWI9 (Glutaminyl-tRNA synthetase OS=Edwardsiella ictaluri (strain 93-146) GN=glnS PE=3 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 2.925e-31
Identity = 67/132 (50.76%), Postives = 94/132 (71.21%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGI-YQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANS 417
            T EF+  R  Y W+L  + I   P  +E+SRLN+  A+MSKRKLN LV+EK V+GWDDPR+ T++GLRRRG ++ A+  F R IG+T+ D   + +A LE  +R++LN+ APR M VLDP+++VI NL A+S
Sbjct:  231 TLEFQDNRRLYDWVLDNISIPCHPRQYEFSRLNLEYAIMSKRKLNLLVSEKIVEGWDDPRMPTISGLRRRGYSAAAVREFCRRIGVTKQDNN-VEMAALESCIRDDLNENAPRAMAVLDPVRLVIDNLAADS 361

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BLAST of GR912371 vs. SwissProt
Match: SYQ_ERWT9 (Glutaminyl-tRNA synthetase OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=glnS PE=3 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 4.989e-31
Identity = 68/131 (51.91%), Postives = 94/131 (71.76%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGI-YQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEAN 414
            T EF+  R  Y W+L  + I   P  +E+SRLN+  A+MSKRKLN LVTEK V+GWDDPR++T++GLRRRG ++ +I  F R IG+T+ D  ++ +A LE  +R++LN+ APR M VLD +KVVI NL A+
Sbjct:  231 TLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYAIMSKRKLNLLVTEKVVEGWDDPRMLTVSGLRRRGYSAGSIREFCRRIGVTKQD-NIVEMAALESCIRDDLNENAPRAMAVLDAVKVVIENLPAH 360

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BLAST of GR912371 vs. SwissProt
Match: SYQ_VIBHB (Glutaminyl-tRNA synthetase OS=Vibrio harveyi (strain ATCC BAA-1116 / BB120) GN=glnS PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 6.515e-31
Identity = 68/140 (48.57%), Postives = 93/140 (66.43%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGI-YQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAKN 441
            T EF   R  Y W+L  + I  +P+ +E+SRLN+   VMSKRKLN+LVTEK VDGWDDPR+ T++GLRRRG  S +I  F + IG+T+ +  +I    LE  +R++LN+ APR M VLDP+K+VI N E  +   +   N
Sbjct:  231 TLEFMDNRRLYDWVLDNITIDCRPHQYEFSRLNLEYTVMSKRKLNQLVTEKLVDGWDDPRMPTVSGLRRRGFTSASIREFCKRIGVTKQE-NMIEFGSLESCIRDDLNENAPRAMAVLDPVKIVIENFEEGAVETLTVAN 369

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BLAST of GR912371 vs. SwissProt
Match: SYQ_KLEP7 (Glutaminyl-tRNA synthetase OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) GN=glnS PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 6.515e-31
Identity = 67/127 (52.76%), Postives = 90/127 (70.87%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGI-YQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITN 402
            T EF+  R  Y W+L  + I   P  +E+SRLN+   VMSKRKLN+LVTEK V+GWDDPR+ T++GLRRRG  + +I  F + IG+T+ D T I +A LE  +RE+LN+ APR M V+DP+K+VI N
Sbjct:  231 TLEFQDNRRLYDWVLDNISIPVHPRQYEFSRLNLEYTVMSKRKLNQLVTEKHVEGWDDPRMPTISGLRRRGYTAESIREFCKRIGVTKQDNT-IEMASLESCIREDLNENAPRAMAVIDPVKLVIEN 356

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BLAST of GR912371 vs. SwissProt
Match: SYQ_KLEP3 (Glutaminyl-tRNA synthetase OS=Klebsiella pneumoniae (strain 342) GN=glnS PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 6.515e-31
Identity = 67/127 (52.76%), Postives = 90/127 (70.87%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGI-YQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITN 402
            T EF+  R  Y W+L  + I   P  +E+SRLN+   VMSKRKLN+LVTEK V+GWDDPR+ T++GLRRRG  + +I  F + IG+T+ D T I +A LE  +RE+LN+ APR M V+DP+K+VI N
Sbjct:  231 TLEFQDNRRLYDWVLDNISIPVHPRQYEFSRLNLEYTVMSKRKLNQLVTEKHVEGWDDPRMPTISGLRRRGYTAESIREFCKRIGVTKQDNT-IEMASLESCIREDLNENAPRAMAVIDPVKLVIEN 356

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BLAST of GR912371 vs. SwissProt
Match: SYQ_CAEEL (Probable glutaminyl-tRNA synthetase OS=Caenorhabditis elegans GN=ers-1 PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 6.515e-31
Identity = 73/137 (53.28%), Postives = 92/137 (67.15%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNL-EANSAIEVD 432
            T EF++RR+SYYWL +AL IY P  WEY RLN++  V+SKRK+ +L+T K V+ WDDPRL TL  LRRRG+ S AIN FV  +G+T S   +I    L+  VR+ LN  APRTM VL+ LK+ I N  E N    VD
Sbjct:  463 TKEFQSRRSSYYWLCNALDIYCPVQWEYGRLNVNYTVVSKRKILKLITTKTVNDWDDPRLFTLTALRRRGIPSEAINRFVAKLGLTMSQ-MVIDPHVLDATVRDYLNIHAPRTMAVLEGLKLTIENFSELNLPSSVD 598

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BLAST of GR912371 vs. TrEMBL
Match: B6UC34_MAIZE (Glutaminyl-tRNA synthetase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 235.343 bits (599), Expect = 1.452e-60
Identity = 113/138 (81.88%), Postives = 124/138 (89.86%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EF+ RR SYYWLL ALG+YQPYVWEYSRLNISN VMSKRKLNRLVTEKWVDGWDDPRL+TLAGLRRRG++STAINSF+RGIGITRSD +LI V RLEYH+REELNKTAPRTMVVL PLKVVITNLE    +++D K
Sbjct:  470 TLEFDIRRPSYYWLLVALGLYQPYVWEYSRLNISNTVMSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVSSTAINSFIRGIGITRSDNSLIRVDRLEYHIREELNKTAPRTMVVLRPLKVVITNLEEGKVLDLDGK 607

[Close]

BLAST of GR912371 vs. TrEMBL
Match: D7T929_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011871001 PE=4 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 2.477e-60
Identity = 110/138 (79.71%), Postives = 126/138 (91.30%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EFETRRASYYWL+ ALG+YQPYVWEYSRLN++N VMSKRKLNRLVTE+WVDGWDDPRLMTLAGLRRRG+ ST+IN+FVRGIGITRSD ++I + RLE+H+REELNKTAPR MVVL PLKVVITNLE  S +++DAK
Sbjct:  466 TLEFETRRASYYWLIDALGLYQPYVWEYSRLNVTNTVMSKRKLNRLVTERWVDGWDDPRLMTLAGLRRRGVTSTSINAFVRGIGITRSDSSMIRLDRLEFHIREELNKTAPRMMVVLHPLKVVITNLEVGSTMDLDAK 603

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BLAST of GR912371 vs. TrEMBL
Match: D7SM09_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_21.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018308001 PE=4 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 2.477e-60
Identity = 111/138 (80.43%), Postives = 125/138 (90.58%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EFETRRASYYWLLH LG+Y PYVWEYSRLN++N VMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRG+ ST+IN+F+RGIGITRSD ++I + RLEY++REELNKTAPRTMVVL PLKVVITNLE  S  ++DAK
Sbjct:  465 TLEFETRRASYYWLLHVLGLYHPYVWEYSRLNVTNTVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGVTSTSINTFIRGIGITRSDCSIIRLDRLEYYIREELNKTAPRTMVVLHPLKVVITNLEDGSITDLDAK 602

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BLAST of GR912371 vs. TrEMBL
Match: A5BJW9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_036765 PE=4 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 2.477e-60
Identity = 111/138 (80.43%), Postives = 125/138 (90.58%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EFETRRASYYWLLH LG+Y PYVWEYSRLN++N VMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRG+ ST+IN+F+RGIGITRSD ++I + RLEY++REELNKTAPRTMVVL PLKVVITNLE  S  ++DAK
Sbjct:  158 TLEFETRRASYYWLLHVLGLYHPYVWEYSRLNVTNTVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGVTSTSINTFIRGIGITRSDCSIIRLDRLEYYIREELNKTAPRTMVVLHPLKVVITNLEDGSITDLDAK 295

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BLAST of GR912371 vs. TrEMBL
Match: C5XMK4_SORBI (Putative uncharacterized protein Sb03g003420 OS=Sorghum bicolor GN=Sb03g003420 PE=3 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 4.226e-60
Identity = 112/138 (81.16%), Postives = 123/138 (89.13%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EF+ RR SYYWLL ALG+YQPYVWEYSRLNISN VMSKRKLNRLVTEKWVDGWDDPRL+TLAGLRRRG++STAINSF+RGIGITRSD +LI V RLEYH+REELNKTAPRTM VL PLKVVITNLE    +++D K
Sbjct:  470 TLEFDIRRPSYYWLLVALGLYQPYVWEYSRLNISNTVMSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVSSTAINSFIRGIGITRSDNSLIRVDRLEYHIREELNKTAPRTMAVLRPLKVVITNLEEGKVLDLDGK 607

[Close]

BLAST of GR912371 vs. TrEMBL
Match: Q8W4F3_ARATH (Putative uncharacterized protein At1g25350 OS=Arabidopsis thaliana GN=At1g25350 PE=2 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.208e-60
Identity = 109/138 (78.99%), Postives = 128/138 (92.75%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EFETRRASYYWLLH+L +Y PYVWEYSRLN++N VMSKRKLN +VT K+VDGWDDPRL+TL+GLRRRG+ STAIN+FVRGIGITRSDG++I V+RLE+H+REELNKTAPRTMVVL+PLKVVITNLE++  IE+DAK
Sbjct:  469 TLEFETRRASYYWLLHSLSLYMPYVWEYSRLNVTNTVMSKRKLNYIVTNKYVDGWDDPRLLTLSGLRRRGVTSTAINAFVRGIGITRSDGSMIHVSRLEHHIREELNKTAPRTMVVLNPLKVVITNLESDKLIELDAK 606

[Close]

BLAST of GR912371 vs. TrEMBL
Match: D7LZB1_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_661834 PE=4 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.208e-60
Identity = 109/138 (78.99%), Postives = 127/138 (92.03%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EFETRRASYYWLLH+LG+Y PYVWEYSRLN++N VMSK KLN +VT K+VDGWDDPRL+TLAGLRRRG+  TAIN+FVRG+GITRSDG+LI V+RLE+H+REELNKTAPRTMVVL+PLKVVITNLEA+  +E+DAK
Sbjct:  459 TLEFETRRASYYWLLHSLGLYMPYVWEYSRLNVTNTVMSKHKLNYIVTNKYVDGWDDPRLLTLAGLRRRGVTPTAINAFVRGLGITRSDGSLIHVSRLEHHIREELNKTAPRTMVVLNPLKVVITNLEADKVMELDAK 596

[Close]

BLAST of GR912371 vs. TrEMBL
Match: A4UVN3_ARATH (tRNA-glutamine synthetase, putative OS=Arabidopsis thaliana GN=F4F7.26 PE=3 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.208e-60
Identity = 109/138 (78.99%), Postives = 128/138 (92.75%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EFETRRASYYWLLH+L +Y PYVWEYSRLN++N VMSKRKLN +VT K+VDGWDDPRL+TL+GLRRRG+ STAIN+FVRGIGITRSDG++I V+RLE+H+REELNKTAPRTMVVL+PLKVVITNLE++  IE+DAK
Sbjct:  460 TLEFETRRASYYWLLHSLSLYMPYVWEYSRLNVTNTVMSKRKLNYIVTNKYVDGWDDPRLLTLSGLRRRGVTSTAINAFVRGIGITRSDGSMIHVSRLEHHIREELNKTAPRTMVVLNPLKVVITNLESDKLIELDAK 597

[Close]

BLAST of GR912371 vs. TrEMBL
Match: B8ADM6_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_00686 PE=3 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 1.606e-59
Identity = 112/138 (81.16%), Postives = 123/138 (89.13%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EF+ RR SYYWLL ALG+YQPYVWEYSRLNISN VMSKRKLNRLVTEKWVDGWDDPRL+TLAGLRRRG++STAINSF+RGIGITRSD +LI V RLEYH+REELNKTA R MVVL+PLKVVITNLE    I++D K
Sbjct:  470 TLEFDIRRPSYYWLLVALGLYQPYVWEYSRLNISNTVMSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVSSTAINSFIRGIGITRSDNSLIRVDRLEYHIREELNKTASRAMVVLNPLKVVITNLEDEKVIDLDGK 607

[Close]

BLAST of GR912371 vs. TrEMBL
Match: D7KAJ6_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_472840 PE=4 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.097e-59
Identity = 108/138 (78.26%), Postives = 127/138 (92.03%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EFETRRA YYWLLH+L +Y PYVWEYSRLN++N VMSKRKLN +VT K+VDGWDDPRL+TL+GLRRRG+ STAIN+FVRGIGITRSDG++I V+RLE+H+REELNKTAPRTMVVL+PLKVVITNLE++  IE+DAK
Sbjct:  469 TLEFETRRAPYYWLLHSLSLYMPYVWEYSRLNVTNTVMSKRKLNYIVTNKYVDGWDDPRLLTLSGLRRRGVTSTAINAFVRGIGITRSDGSMIHVSRLEHHIREELNKTAPRTMVVLNPLKVVITNLESDKVIELDAK 606

[Close]

BLAST of GR912371 vs. TAIR peptide
Match: AT1G25350.2 (| Symbols: OVA9 | glutamine-tRNA ligase, putative / glutaminyl-tRNA synthetase, putative / GlnRS, putative | chr1:8889280-8894205 REVERSE LENGTH=800)

HSP 1 Score: 233.032 bits (593), Expect = 5.393e-62
Identity = 109/138 (78.99%), Postives = 128/138 (92.75%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EFETRRASYYWLLH+L +Y PYVWEYSRLN++N VMSKRKLN +VT K+VDGWDDPRL+TL+GLRRRG+ STAIN+FVRGIGITRSDG++I V+RLE+H+REELNKTAPRTMVVL+PLKVVITNLE++  IE+DAK
Sbjct:  474 TLEFETRRASYYWLLHSLSLYMPYVWEYSRLNVTNTVMSKRKLNYIVTNKYVDGWDDPRLLTLSGLRRRGVTSTAINAFVRGIGITRSDGSMIHVSRLEHHIREELNKTAPRTMVVLNPLKVVITNLESDKLIELDAK 611

[Close]

BLAST of GR912371 vs. TAIR peptide
Match: AT1G25350.1 (| Symbols: OVA9 | glutamine-tRNA ligase, putative / glutaminyl-tRNA synthetase, putative / GlnRS, putative | chr1:8889280-8894205 REVERSE LENGTH=795)

HSP 1 Score: 233.032 bits (593), Expect = 5.393e-62
Identity = 109/138 (78.99%), Postives = 128/138 (92.75%), Query Frame = 1

Query:   25 TFEFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVITNLEANSAIEVDAK 438
            T EFETRRASYYWLLH+L +Y PYVWEYSRLN++N VMSKRKLN +VT K+VDGWDDPRL+TL+GLRRRG+ STAIN+FVRGIGITRSDG++I V+RLE+H+REELNKTAPRTMVVL+PLKVVITNLE++  IE+DAK
Sbjct:  469 TLEFETRRASYYWLLHSLSLYMPYVWEYSRLNVTNTVMSKRKLNYIVTNKYVDGWDDPRLLTLSGLRRRGVTSTAINAFVRGIGITRSDGSMIHVSRLEHHIREELNKTAPRTMVVLNPLKVVITNLESDKLIELDAK 606

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BLAST of GR912371 vs. TAIR peptide
Match: AT5G26710.1 (| Symbols: | Glutamyl/glutaminyl-tRNA synthetase, class Ic | chr5:9305673-9308247 FORWARD LENGTH=719)

HSP 1 Score: 69.707 bits (169), Expect = 7.897e-13
Identity = 41/123 (33.33%), Postives = 68/123 (55.28%), Query Frame = 1

Query:   31 EFETRRASYYWLLHALGIYQPYVWEYSRLNISNAVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGMASTAINSFVRGIGITRSDGTLISVARLEYHVREELNKTAPRTMVVLDPLKVVIT 399
            E+  R A Y+ +L  +G+ Q  ++E+SRLN+   ++SKRKL   V    VDGWDDPR  T+ G+ RRG+   A+  F+   G ++ +  L+   +L    +  ++   PR   V+   +V+ T
Sbjct:  414 EYHDRNAQYFKVLEDMGLRQVQLYEFSRLNLVFTLLSKRKLLWFVQTGLVDGWDDPRFPTVQGIVRRGLKIEALIQFILEQGASK-NLNLMEWDKLWSINKRIIDPVCPRHTAVVAERRVLFT 535

The following BLAST results are available for this feature:

BLAST of GR912371 vs. SwissProt
BLAST of GR912371 vs. TrEMBL
BLAST of GR912371 vs. TAIR peptide

BLAST of GR912371 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SYQ_LUPLU	6.582e-69	92.03	Glutaminyl-tRNA synthetase OS=Lupinus luteus PE=2 ...	[more]
SYQ_DICDI	2.826e-34	51.08	Probable glutaminyl-tRNA synthetase OS=Dictyosteli...	[more]
SYQ_DROME	5.330e-33	51.47	Probable glutaminyl-tRNA synthetase OS=Drosophila ...	[more]
SYQ_BOVIN	5.893e-32	51.08	Glutaminyl-tRNA synthetase OS=Bos taurus GN=QARS P...	[more]
SYQ_EDWI9	2.925e-31	50.76	Glutaminyl-tRNA synthetase OS=Edwardsiella ictalur...	[more]
SYQ_ERWT9	4.989e-31	51.91	Glutaminyl-tRNA synthetase OS=Erwinia tasmaniensis...	[more]
SYQ_VIBHB	6.515e-31	48.57	Glutaminyl-tRNA synthetase OS=Vibrio harveyi (stra...	[more]
SYQ_KLEP7	6.515e-31	52.76	Glutaminyl-tRNA synthetase OS=Klebsiella pneumonia...	[more]
SYQ_KLEP3	6.515e-31	52.76	Glutaminyl-tRNA synthetase OS=Klebsiella pneumonia...	[more]
SYQ_CAEEL	6.515e-31	53.28	Probable glutaminyl-tRNA synthetase OS=Caenorhabdi...	[more]

BLAST of GR912371 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B6UC34_MAIZE	1.452e-60	81.88	Glutaminyl-tRNA synthetase OS=Zea mays PE=2 SV=1	[more]
D7T929_VITVI	2.477e-60	79.71	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7SM09_VITVI	2.477e-60	80.43	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BJW9_VITVI	2.477e-60	80.43	Putative uncharacterized protein OS=Vitis vinifera...	[more]
C5XMK4_SORBI	4.226e-60	81.16	Putative uncharacterized protein Sb03g003420 OS=So...	[more]
Q8W4F3_ARATH	7.208e-60	78.99	Putative uncharacterized protein At1g25350 OS=Arab...	[more]
D7LZB1_ARALY	7.208e-60	78.99	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
A4UVN3_ARATH	7.208e-60	78.99	tRNA-glutamine synthetase, putative OS=Arabidopsis...	[more]
B8ADM6_ORYSI	1.606e-59	81.16	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7KAJ6_ARALY	2.097e-59	78.26	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of GR912371 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G25350.2	5.393e-62	78.99	\| Symbols: OVA9 \| glutamine-tRNA ligase, putative ...	[more]
AT1G25350.1	5.393e-62	78.99	\| Symbols: OVA9 \| glutamine-tRNA ligase, putative ...	[more]
AT5G26710.1	7.897e-13	33.33	\| Symbols: \| Glutamyl/glutaminyl-tRNA synthetase,...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000924	Glutamyl/glutaminyl-tRNA synthetase, class Ic	PANTHER	PTHR10119	GLUTAMYL/GLUTAMINYL-TRNA SYNTHETASE	coord: 7..145 score: 8.3
IPR004514	Glutaminyl-tRNA synthetase, class Ic	PANTHER	PTHR10119:SF3	GLUTAMINYL-TRNA SYNTHETASE (GLUTAMINE--TRNA LIGASE) (GLNRS)	coord: 7..145 score: 8.3
IPR020058	Glutamyl/glutaminyl-tRNA synthetase, class Ic, catalytic domain	PFAM	PF00749	tRNA-synt_1c	coord: 7..113 score: 1.7
IPR020061	Glutamyl/glutaminyl-tRNA synthetase, class Ic, alpha-bundle domain	GENE3D	1.10.1160.10	no description	coord: 36..114 score: 6.8
None	No IPR available	SUPERFAMILY	52374	Nucleotidylyl transferase	coord: 6..113 score: 5

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR912371 ID=GR912371; Name=GR912371; organism=Cicer arietinum; type=EST; length=460bp
GATTAGCGTGGTCGCGGCCGAGGTACTTTTGAGTTTGAGACACGCCGTGC
TTCATATTATTGGTTGTTACACGCATTAGGTATCTACCAACCTTATGTAT
GGGAATATTCAAGGTTAAATATCTCTAACGCTGTTATGTCAAAGCGCAAG
CTTAATCGCCTAGTTACTGAAAAGTGGGTTGATGGGTGGGATGATCCTCG
CCTGATGACATTAGCCGGATTGCGGCGTAGAGGCATGGCTTCAACAGCAA
TCAATTCTTTTGTTCGAGGAATTGGAATCACTAGAAGTGATGGCACTTTG
ATATCTGTGGCTCGCCTTGAATATCATGTTAGGGAAGAATTGAACAAAAC
AGCTCCTCGGACAATGGTTGTCCTAGATCCACTCAAGGTTGTTATTACTA
ATCTTGAAGCCAACTCAGCCATTGAGGTTGATGCAAAAAATGGCCTGATG
CTCAAGCTGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000166	nucleotide binding
GO:0005524	ATP binding
GO:0004819	glutamine-tRNA ligase activity
GO:0004812	aminoacyl-tRNA ligase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation
GO:0043039	tRNA aminoacylation
GO:0006425	glutaminyl-tRNA aminoacylation
GO:0006418	tRNA aminoacylation for protein translation

Vocabulary: **INTERPRO**
Term	Definition
IPR000924	Glu/Gln-tRNA-synth
IPR004514	Gln-tRNA-synth
IPR020058	Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020061	Glu_tRNA_lig_a-bdl

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