GR408982

EST Overview


Name	GR408982
Unique Name	GR408982
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	739

Libraries


Library Name	Type
LIBEST_024778 ICC1882 field drought stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR408982 vs. SwissProt
Match: DEF_NELNU (Defensin-like protein OS=Nelumbo nucifera PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.146e-8
Identity = 30/65 (46.15%), Postives = 38/65 (58.46%), Query Frame = 2

Query:   41 MARSIPMDSTIXXXXXXXXXXEMG---AETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            M R + + S++          EMG   AE RTCES+  +FKG C SD NCA VC+TE FP G C+
Sbjct:    1 MERGMRLFSSLVLVLLLVTATEMGPKVAEARTCESQSHRFKGACLSDTNCASVCQTEGFPAGDCK 65

[Close]

BLAST of GR408982 vs. SwissProt
Match: DFSD2_HELAN (Defensin SD2 OS=Helianthus annuus GN=SD2 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.954e-8
Identity = 31/66 (46.97%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   41 MARSIPMDSTIXXXXXXXXXXEMGA----ETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            M  S+ M + +          EMG     E RTCES+  KFKG C SD NCA VC +ERF GG CR
Sbjct:    1 MKSSMKMFAALLLVVMCLLANEMGGPLVVEARTCESQSHKFKGTCLSDTNCANVCHSERFSGGKCR 66

[Close]

BLAST of GR408982 vs. SwissProt
Match: DEF_PETIN (Defensin-like protein OS=Petunia integrifolia PE=2 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 9.699e-8
Identity = 30/66 (45.45%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   41 MARSIPMDSTIXXXXXXXXXXEMG----AETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            M RSI + +T           EMG    AE RTCES+  +F G C  + NCA VC+TE F GG CR
Sbjct:    1 MGRSIRLFATFFLIAMLFLSTEMGPMTSAEARTCESQSHRFHGTCVRESNCASVCQTEGFIGGNCR 66

[Close]

BLAST of GR408982 vs. SwissProt
Match: DEF04_ARATH (Defensin-like protein 4 OS=Arabidopsis thaliana GN=PDF2.1 PE=2 SV=2)

HSP 1 Score: 55.8398 bits (133), Expect = 2.822e-7
Identity = 23/37 (62.16%), Postives = 26/37 (70.27%), Query Frame = 2

Query:  116 ETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            E RTC S+ Q+FKG+C SD NC  VC  E FPGG CR
Sbjct:   29 EARTCASQSQRFKGKCVSDTNCENVCHNEGFPGGDCR 65

[Close]

BLAST of GR408982 vs. SwissProt
Match: DF322_SOLTU (Defensin-like protein P322 OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 3.686e-7
Identity = 24/37 (64.86%), Postives = 25/37 (67.57%), Query Frame = 2

Query:  113 AETRTCESKRQKFKGECASDRNCAFVCETERFPGGYC 223
            AE R CES   +FKG C  D NCA VCETERF GG C
Sbjct:   25 AEARHCESLSHRFKGPCTRDSNCASVCETERFSGGNC 61

[Close]

BLAST of GR408982 vs. SwissProt
Match: DEF2_TRIKH (Defensin Tk-AMP-D2 OS=Triticum kiharae PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 6.287e-7
Identity = 23/34 (67.65%), Postives = 24/34 (70.59%), Query Frame = 2

Query:  122 RTCESKRQKFKGECASDRNCAFVCETERFPGGYC 223
            RTCES+  KFKG C SD NCA VC TE FP G C
Sbjct:    1 RTCESQSHKFKGPCFSDSNCATVCRTENFPRGQC 34

[Close]

BLAST of GR408982 vs. SwissProt
Match: DEF2_CAPAN (Defensin J1-2 OS=Capsicum annuum PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 6.287e-7
Identity = 24/40 (60.00%), Postives = 27/40 (67.50%), Query Frame = 2

Query:  107 MGAETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            M AE RTCES+  +FKG C S  NC  VC TE F GG+CR
Sbjct:   23 MVAEARTCESQSHRFKGLCFSKSNCGSVCHTEGFNGGHCR 62

[Close]

BLAST of GR408982 vs. TrEMBL
Match: C1K3M7_VIGUN (Defensin OS=Vigna unguiculata GN=PDEF PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 8.908e-12
Identity = 37/66 (56.06%), Postives = 42/66 (63.64%), Query Frame = 2

Query:   41 MARSIPMDSTIXXXXXXXXXXEMG----AETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            MARS+P+ STI          EMG    AE RTCES+  +FKG C SD NCA VC TERF GG+CR
Sbjct:    1 MARSVPLVSTIFVFLLLLVATEMGPTMVAEARTCESQSHRFKGPCVSDTNCASVCRTERFSGGHCR 66

[Close]

BLAST of GR408982 vs. TrEMBL
Match: Q39807_SOYBN (Protease inhibitor OS=Glycine max PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.089e-9
Identity = 34/67 (50.75%), Postives = 40/67 (59.70%), Query Frame = 2

Query:   41 MARSIPMDSTIXXXXXXXXXXEMG-----AETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            M+RS+P+ STI          EM      AE RTCES+  +FKG C SD NC  VC TERF GG+CR
Sbjct:    1 MSRSVPLVSTICVLLLLLVATEMMGPTMVAEARTCESQSHRFKGPCLSDTNCGSVCRTERFTGGHCR 67

[Close]

BLAST of GR408982 vs. TrEMBL
Match: B1N681_SOLPI (Defensin protein OS=Solanum pimpinellifolium GN=SUNPIM_69t000002 PE=4 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 2.054e-8
Identity = 33/66 (50.00%), Postives = 38/66 (57.58%), Query Frame = 2

Query:   41 MARSIPMDSTIXXXXXXXXXXEMG----AETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            MA+SI   +T+          EMG     E R CES   +FKG C SD+NCA VCETERF GG CR
Sbjct:    1 MAQSIRFFATLFLLAMLVMATEMGPTRIVEARHCESLSHRFKGPCVSDKNCASVCETERFSGGNCR 66

[Close]

BLAST of GR408982 vs. TrEMBL
Match: Q84UH1_PRUPE (Defensin protein 1 OS=Prunus persica GN=DFN1 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 5.975e-8
Identity = 26/40 (65.00%), Postives = 30/40 (75.00%), Query Frame = 2

Query:  107 MGAETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            M AE RTCES+  +FKG C S  NCA VC+TE FPGG+CR
Sbjct:   28 MVAEARTCESQSNRFKGTCVSTSNCASVCQTEGFPGGHCR 67

[Close]

BLAST of GR408982 vs. TrEMBL
Match: B2BGR0_OLEEU (Putative defensin protein 1 (Fragment) OS=Olea europaea PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.739e-7
Identity = 25/40 (62.50%), Postives = 29/40 (72.50%), Query Frame = 2

Query:  107 MGAETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            M A+ RTCES+  +FKG C S  NCA VC+TE FP GYCR
Sbjct:    5 MVADARTCESQSHRFKGSCVSKSNCAAVCQTEGFPDGYCR 44

[Close]

BLAST of GR408982 vs. TrEMBL
Match: Q9MB66_TOBAC (Thionin like protein OS=Nicotiana tabacum GN=Nt-thionin PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 5.058e-7
Identity = 31/66 (46.97%), Postives = 37/66 (56.06%), Query Frame = 2

Query:   41 MARSIPMDSTIXXXXXXXXXXEMG----AETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            MA S+   +T+          EMG    AE RTCES+  +FKG C+ D NCA VC TE F GG CR
Sbjct:    1 MANSMRFFATVLLIALLVTATEMGPMTIAEARTCESQSHRFKGPCSRDSNCATVCLTEGFSGGDCR 66

[Close]

BLAST of GR408982 vs. TrEMBL
Match: Q9XG53_SOLLC (Gamma-thionin OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 8.628e-7
Identity = 24/37 (64.86%), Postives = 28/37 (75.68%), Query Frame = 2

Query:  116 ETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            E RTCES+  +FKG C S++NCA VCETE F GG CR
Sbjct:   24 EARTCESQSHRFKGPCVSEKNCASVCETEGFSGGDCR 60

[Close]

BLAST of GR408982 vs. TrEMBL
Match: Q8W4V6_CAPAN (Defensin OS=Capsicum annuum PE=3 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 8.628e-7
Identity = 30/66 (45.45%), Postives = 37/66 (56.06%), Query Frame = 2

Query:   41 MARSIPMDSTIXXXXXXXXXXEMG----AETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            MA S+   + +          EMG     E RTCES+  +FKG CAS+ NCA VC+TE F GG CR
Sbjct:    1 MAHSMRFFAIVLLLAMLVMATEMGPMRIVEARTCESQSHRFKGVCASETNCASVCQTEGFSGGDCR 66

[Close]

BLAST of GR408982 vs. TrEMBL
Match: Q7F8K7_ORYSJ (ESTs AU069800(E3445) OS=Oryza sativa subsp. japonica GN=Os03g0130300 PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 8.628e-7
Identity = 25/39 (64.10%), Postives = 27/39 (69.23%), Query Frame = 2

Query:  107 MGAETRTCESKRQKFKGECASDRNCAFVCETERFPGGYC 223
            M AE RTCES+  +FKG CA   NCA VC TE FP GYC
Sbjct:   29 MVAEARTCESQSHRFKGPCARKANCASVCNTEGFPDGYC 67

[Close]

BLAST of GR408982 vs. TrEMBL
Match: O24225_ORYSA (Proteinase inhibitor OS=Oryza sativa GN=RPI PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 8.628e-7
Identity = 25/39 (64.10%), Postives = 27/39 (69.23%), Query Frame = 2

Query:  107 MGAETRTCESKRQKFKGECASDRNCAFVCETERFPGGYC 223
            M AE RTCES+  +FKG CA   NCA VC TE FP GYC
Sbjct:   29 MVAEARTCESQSHRFKGPCARKANCASVCNTEGFPDGYC 67

[Close]

BLAST of GR408982 vs. TAIR peptide
Match: AT2G02120.1 (| Symbols: PDF2.1, LCR70 | Scorpion toxin-like knottin superfamily protein | chr2:538319-538842 FORWARD LENGTH=77)

HSP 1 Score: 55.8398 bits (133), Expect = 2.809e-8
Identity = 23/37 (62.16%), Postives = 26/37 (70.27%), Query Frame = 2

Query:  116 ETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            E RTC S+ Q+FKG+C SD NC  VC  E FPGG CR
Sbjct:   29 EARTCASQSQRFKGKCVSDTNCENVCHNEGFPGGDCR 65

[Close]

BLAST of GR408982 vs. TAIR peptide
Match: AT2G02130.1 (| Symbols: PDF2.3, LCR68 | low-molecular-weight cysteine-rich 68 | chr2:540071-540407 FORWARD LENGTH=77)

HSP 1 Score: 53.5286 bits (127), Expect = 1.394e-7
Identity = 23/37 (62.16%), Postives = 24/37 (64.86%), Query Frame = 2

Query:  116 ETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            E RTCESK  +FKG C S  NCA VC  E F GG CR
Sbjct:   29 EARTCESKSHRFKGPCVSTHNCANVCHNEGFGGGKCR 65

[Close]

BLAST of GR408982 vs. TAIR peptide
Match: AT2G02100.1 (| Symbols: LCR69, PDF2.2 | low-molecular-weight cysteine-rich 69 | chr2:528397-528885 FORWARD LENGTH=77)

HSP 1 Score: 51.2174 bits (121), Expect = 6.919e-7
Identity = 22/37 (59.46%), Postives = 24/37 (64.86%), Query Frame = 2

Query:  116 ETRTCESKRQKFKGECASDRNCAFVCETERFPGGYCR 226
            E RTCES+  +FKG C S  NCA VC  E F GG CR
Sbjct:   29 EARTCESQSHRFKGTCVSASNCANVCHNEGFVGGNCR 65

The following BLAST results are available for this feature:

BLAST of GR408982 vs. SwissProt
BLAST of GR408982 vs. TrEMBL
BLAST of GR408982 vs. TAIR peptide

BLAST of GR408982 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
DEF_NELNU	1.146e-8	46.15	Defensin-like protein OS=Nelumbo nucifera PE=3 SV=...	[more]
DFSD2_HELAN	1.954e-8	46.97	Defensin SD2 OS=Helianthus annuus GN=SD2 PE=2 SV=1	[more]
DEF_PETIN	9.699e-8	45.45	Defensin-like protein OS=Petunia integrifolia PE=2...	[more]
DEF04_ARATH	2.822e-7	62.16	Defensin-like protein 4 OS=Arabidopsis thaliana GN...	[more]
DF322_SOLTU	3.686e-7	64.86	Defensin-like protein P322 OS=Solanum tuberosum PE...	[more]
DEF2_TRIKH	6.287e-7	67.65	Defensin Tk-AMP-D2 OS=Triticum kiharae PE=1 SV=1	[more]
DEF2_CAPAN	6.287e-7	60.00	Defensin J1-2 OS=Capsicum annuum PE=1 SV=1	[more]

BLAST of GR408982 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C1K3M7_VIGUN	8.908e-12	56.06	Defensin OS=Vigna unguiculata GN=PDEF PE=3 SV=1	[more]
Q39807_SOYBN	1.089e-9	50.75	Protease inhibitor OS=Glycine max PE=4 SV=1	[more]
B1N681_SOLPI	2.054e-8	50.00	Defensin protein OS=Solanum pimpinellifolium GN=SU...	[more]
Q84UH1_PRUPE	5.975e-8	65.00	Defensin protein 1 OS=Prunus persica GN=DFN1 PE=4 ...	[more]
B2BGR0_OLEEU	1.739e-7	62.50	Putative defensin protein 1 (Fragment) OS=Olea eur...	[more]
Q9MB66_TOBAC	5.058e-7	46.97	Thionin like protein OS=Nicotiana tabacum GN=Nt-th...	[more]
Q9XG53_SOLLC	8.628e-7	64.86	Gamma-thionin OS=Solanum lycopersicum PE=4 SV=1	[more]
Q8W4V6_CAPAN	8.628e-7	45.45	Defensin OS=Capsicum annuum PE=3 SV=1	[more]
Q7F8K7_ORYSJ	8.628e-7	64.10	ESTs AU069800(E3445) OS=Oryza sativa subsp. japoni...	[more]
O24225_ORYSA	8.628e-7	64.10	Proteinase inhibitor OS=Oryza sativa GN=RPI PE=4 S...	[more]

BLAST of GR408982 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT2G02120.1	2.809e-8	62.16	\| Symbols: PDF2.1, LCR70 \| Scorpion toxin-like kno...	[more]
AT2G02130.1	1.394e-7	62.16	\| Symbols: PDF2.3, LCR68 \| low-molecular-weight cy...	[more]
AT2G02100.1	6.919e-7	59.46	\| Symbols: LCR69, PDF2.2 \| low-molecular-weight cy...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008176	Gamma thionin	PFAM	PF00304	Gamma-thionin	coord: 37..73 score: 3.2
IPR008177	Gamma Purothionin	PRINTS	PR00288	PUROTHIONIN	coord: 38..53 score: 6.1e-05coord: 54..67 score: 6.1
None	No IPR available	GENE3D	3.30.30.10	no description	coord: 37..74 score: 1
None	No IPR available	SEG	seg	seg	coord: 21..30 score
None	No IPR available	SUPERFAMILY	57095	Scorpion toxin-like	coord: 37..73 score: 1.1
None	No IPR available	SEG	seg	seg	coord: 8..18 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR408982 ID=GR408982; Name=GR408982; organism=Cicer arietinum; type=EST; length=739bp
GACTCCAAACTAGTTCTCTTTTTATTTCTTTGAAATTAACATGGCTCGTT
CAATTCCTATGGATTCCACCATCTTTGTCTTCCTTCTGCTTCTTGTAACT
ACTGAGATGGGGGCAGAGACAAGGACATGTGAGTCAAAAAGACAAAAATT
CAAAGGAGAATGTGCGAGTGATCGCAATTGTGCTTTTGTGTGCGAAACGG
AACGTTTTCCTGGAGGGTATTGCCGTGTGATAGAGTCCTCTATGCTTCCG
CACTACACTTTGATAATTAATGTTAGAACAGCTCCCCACTACGCCCCCAG
AATTCACAATAAGACTGAGCCCTCTCTCTTCTACGACTAGAACCGATTTT
GTAATTATGCAACCGATACCATGAATGAAAATAATTAACGCCCGCGCCCG
ATTAAGTTACTCATATTGTATCATTTAACAAATTGAGGAGGTGGGGTTTT
TATGCCGCCCCCGCGCGCAATAAATCTGTAGGTTATTTTTTGCTCCAAAC
TACTACCTTTATTATTTTTATTCCGAAATTGTTTTGGTACCCAATTCTAC
TATTCCCATTCTTCTATTGCACTGCCCCCCATTAAATTATTTCACTGGTG
CTCCACGTGTGGAAGTCAAATGACGTCCTACCCTCCCTTTTAGAATATTC
CCCCCTTGGTTTTTACGCTCTCAAAAACCCTTTTGGAAACGCCGAACCCC
TCTTTTTATATTAGTACCTTTCAGTACCCACTCAATTAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006952	defense response

Vocabulary: **INTERPRO**
Term	Definition
IPR008176	Defensin_plant
IPR008177	G_Purothionin

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