CK148866

EST Overview


Name	CK148866
Unique Name	CK148866
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	639

Libraries


Library Name	Type
LIBEST_014674 Suppressive subtractive hybridization library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of CK148866 vs. SwissProt
Match: PUR4_ARATH (Probable phosphoribosylformylglycinamidine synthase, chloroplastic OS=Arabidopsis thaliana GN=At1g74260 PE=1 SV=1)

HSP 1 Score: 347.051 bits (889), Expect = 4.681e-95
Identity = 168/208 (80.77%), Postives = 186/208 (89.42%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEP+IQG+ RTFGMRLPSGDRREWLKPIMFSAGIGQIDH HI+KGEP++GMLVVKIGGPAYRI             QNDAELDFNAVQRGDAEM+QKLYR+VR CIEMG+KNPIISIHDQGAGGNCNVVKEIIYP+GAEID+R++VVGDHTMSVLEIWGAEYQEQDAILVK ES E+L+SI KRE++SMAVIGTI+G GR  L+DS
Sbjct:  460 KFGEPMIQGYTRTFGMRLPSGDRREWLKPIMFSAGIGQIDHTHITKGEPEVGMLVVKIGGPAYRIGMGGGAASSMVSGQNDAELDFNAVQRGDAEMSQKLYRVVRACIEMGEKNPIISIHDQGAGGNCNVVKEIIYPQGAEIDIRAVVVGDHTMSVLEIWGAEYQEQDAILVKAESREILQSICKRERLSMAVIGTINGGGRCTLIDS 667

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BLAST of CK148866 vs. SwissProt
Match: PUR4_DICDI (Phosphoribosylformylglycinamidine synthase OS=Dictyostelium discoideum GN=purL PE=1 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 4.000e-62
Identity = 116/209 (55.50%), Postives = 152/209 (72.73%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEM---GDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLV 631
            KFGEP+I GF R++G  LP+G+RREW+KPIMFS GIG +D  H+ K +P+IGM+VVK GGPAYRI              N  ELDF+AVQRGDAEM QKL R+VR+C+E    G  NPI+S+HDQGAGG  NV+KEI+ P GA+I +  I+ GD T+S +EIWGAEYQE DA+L+K E  + LK +S+RE++ +A +G ++GDG   L+
Sbjct:  399 KFGEPVIIGFTRSYGNTLPNGERREWIKPIMFSGGIGFMDERHLKKEQPEIGMVVVKAGGPAYRIGMGGGSASSMVGGDNKHELDFSAVQRGDAEMGQKLNRIVRSCVESEIHGGCNPIVSVHDQGAGGAGNVLKEIVDPLGAKIYLDRIISGDPTLSAMEIWGAEYQENDALLIKAEHKDYLKKVSERERLPIAFVGDVTGDGIAQLI 607

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BLAST of CK148866 vs. SwissProt
Match: PUR4_MOUSE (Phosphoribosylformylglycinamidine synthase OS=Mus musculus GN=Pfas PE=2 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 8.631e-57
Identity = 107/208 (51.44%), Postives = 140/208 (67.31%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXX-QNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVD 634
            KFGEP++ GF R+ G++LP G RREW+KPIMFS GIG ++  H+ K  P+ GM VVK+GGP YRI               N ++LDF AVQRGD EM QK+ R++R C+E    NPI S+HDQGAGGN NV+KE+  P+GA I      +GD T++ LEIWGAEYQE +A+L++P   + L   S RE+     +GTI+GD R+VLVD
Sbjct:  395 KFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEAKHVGKKPPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPEGAIIYTSRFQLGDPTLNALEIWGAEYQESNALLLRPSDRDFLSRASARERCPACFVGTITGDKRIVLVD 602

[Close]

BLAST of CK148866 vs. SwissProt
Match: PUR4_HUMAN (Phosphoribosylformylglycinamidine synthase OS=Homo sapiens GN=PFAS PE=1 SV=3)

HSP 1 Score: 218.394 bits (555), Expect = 2.511e-56
Identity = 108/208 (51.92%), Postives = 141/208 (67.79%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXX-QNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVD 634
            KFGEP++ GF R+ G++LP G RREW+KPIMFS GIG ++  HISK  P+ GM VVK+GGP YRI               N ++LDF AVQRGD EM QK+ R++R C+E    NPI S+HDQGAGGN NV+KE+  P GA I      +GD T++ LEIWGAEYQE +A+L++  + + L  +S RE+     +GTI+GD R+VLVD
Sbjct:  395 KFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHISKEAPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPKGNPICSLHDQGAGGNGNVLKELSDPAGAIIYTSRFQLGDPTLNALEIWGAEYQESNALLLRSPNRDFLTHVSARERCPACFVGTITGDRRIVLVD 602

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BLAST of CK148866 vs. SwissProt
Match: PUR4_DROME (Phosphoribosylformylglycinamidine synthase OS=Drosophila melanogaster GN=ade2 PE=1 SV=2)

HSP 1 Score: 196.823 bits (499), Expect = 7.825e-50
Identity = 102/212 (48.11%), Postives = 140/212 (66.04%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMR--LPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXX-QNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYP--KGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVD 634
            KFGEP+I GF  ++G+     +  R E++KPIMFS G+G +      K  P  G L+ KIGGP YRI                DAELDFNAVQRGDAEM  KL R+VR C+++G++NPI++IHDQGAGGN NV+KE++ P   GA I  +   +GD T++ LE+WGAEYQE +AIL   +  ELL+ I +RE+  ++ +G ++GDGRV L++
Sbjct:  400 KFGEPVISGFALSYGLNSAADASQRDEYVKPIMFSGGLGTMPATMREKLPPARGQLLAKIGGPVYRIGVGGGAASSVEIQGSGDAELDFNAVQRGDAEMENKLNRVVRACLDLGEQNPILAIHDQGAGGNGNVLKELVEPGFAGAVIFSKEFQLGDPTITALELWGAEYQENNAILCNADQRELLEKICRRERCPISFVGVVTGDGRVTLLE 611

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BLAST of CK148866 vs. SwissProt
Match: PUR4_CAEEL (Probable phosphoribosylformylglycinamidine synthase OS=Caenorhabditis elegans GN=F10F2.2 PE=2 SV=2)

HSP 1 Score: 188.348 bits (477), Expect = 2.783e-47
Identity = 101/207 (48.79%), Postives = 133/207 (64.25%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQN-DAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLV 631
            KFGEP+I GF R+FG RL +G+R E+LKPIMFS GIG ID   + K        VVKIGGP YRI              N + +LDF AVQRGDAEM  KL+R+VR C E    NP+++IHDQGAGGN NV+KE++   G  +   +  +GD ++S+ E+W AEYQE DA LV     + L++ISKREK  ++V+G +  + RV L+
Sbjct:  387 KFGEPVISGFARSFGQRLENGERCEYLKPIMFSGGIGAIDKDEVRKEPCAPHQKVVKIGGPVYRIGVGGGAASSVSVQGNRENQLDFAAVQRGDAEMGGKLHRVVRACAERIGGNPLMAIHDQGAGGNGNVIKELVEGCGVTVKSDTFQLGDESISLRELWTAEYQENDAALVDASLLDALQTISKREKCHVSVVGEVEKEQRVKLL 593

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BLAST of CK148866 vs. SwissProt
Match: PUR4_NEIMA (Phosphoribosylformylglycinamidine synthase OS=Neisseria meningitidis serogroup A GN=purL PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 1.483e-32
Identity = 75/209 (35.89%), Postives = 118/209 (56.46%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYP--KGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVD 634
            +FG P + G+ RTF  +   G  R + KPIM + G+G I      K E   G L++++GGP   I              NDA LDFN+VQRG+ E+ ++   ++  C ++GDKNPIISIHD GAGG  N   E++    +GA   +R + + +H ++ L+IW  E QE+  + +  +  ++ +SI +RE+   AV+GT + DG + + D
Sbjct:  383 EFGRPNLLGYFRTFEEKF-DGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGGGAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGLSNAFPELVNDAGRGAVFKLREVPLEEHGLNPLQIWCNESQERYVLSILEKDLDIFRSICERERCPFAVVGTATDDGHLKVRD 590

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BLAST of CK148866 vs. SwissProt
Match: PUR4_HAES1 (Phosphoribosylformylglycinamidine synthase OS=Haemophilus somnus (strain 129Pt) GN=purL PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 5.634e-32
Identity = 75/213 (35.21%), Postives = 120/213 (56.34%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRRE---WLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYP--KGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            +FG P + G+ RT+  ++ S +  E   + KPIM + GIG I H H+ KGE  +G  ++ +GG A  I             ++  +LDF +VQR + EM ++   ++  C ++G +NPI+ IHD GAGG  N + E+++   +G   ++R I+  +  MS LEIW  E QE+  + V PE   L ++I +RE+ S AVIG  +   ++ L DS
Sbjct:  379 EFGRPALLGYFRTYEEKVNSFNGEEVRGYHKPIMLAGGIGNIRHEHVQKGEIPVGAKLIVLGGAAMNIGLGGGAASSMASGKSKEDLDFASVQRDNPEMERRCQEVIDRCWQLGSENPILFIHDVGAGGLSNAMPELVHDGGRGGRFELRKILCDEKGMSPLEIWCNESQERYVLAVSPEKLPLFEAICQRERASFAVIGEATEQQQLTLQDS 591

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BLAST of CK148866 vs. SwissProt
Match: PUR4_NEIG1 (Phosphoribosylformylglycinamidine synthase OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=purL PE=3 SV=2)

HSP 1 Score: 136.732 bits (343), Expect = 9.609e-32
Identity = 73/209 (34.93%), Postives = 118/209 (56.46%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYP--KGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVD 634
            +FG P + G+ RTF  +   G  R + KPIM + G+G I      K E   G L++++GGP   I              NDA LDFN+VQRG+ E+ ++   ++  C ++GD+NPIISIHD GAGG  N   E++    +GA  ++R + + +H ++ L+IW  E QE+  + +  +  +  ++I +RE+   AV+GT + DG + + D
Sbjct:  379 EFGRPNLLGYFRTFEEKF-DGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGGGAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDQNPIISIHDVGAGGLSNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAICERERCPFAVVGTATDDGHLKVRD 586

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BLAST of CK148866 vs. SwissProt
Match: PUR4_PHOLL (Phosphoribosylformylglycinamidine synthase OS=Photorhabdus luminescens subsp. laumondii GN=purL PE=3 SV=2)

HSP 1 Score: 136.346 bits (342), Expect = 1.255e-31
Identity = 73/212 (34.43%), Postives = 119/212 (56.13%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPS---GDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYP--KGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVD 634
            +FG P + G+ RT+  ++ S    + R + KPIM + GIG I   H+ KGE  +G  ++ +GGP+  I             Q+DA+LDF +VQR + EM ++   ++  C ++G+ NPI+ IHD GAGG  N + E++    +G   ++R I+  +  MS LE+W  E QE+  + V PE   L + I +RE+   A+IG  + +  ++L D
Sbjct:  378 EFGRPALLGYFRTYEEKVNSHNGSELRGYHKPIMLAGGIGNIRDEHVKKGEISVGAKLIVLGGPSMNIGLGGGAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGENNPILFIHDVGAGGLSNAMPELVSDGGRGGRFELRKILNDEPGMSPLEVWCNESQERYVLAVAPEQLPLFEEICRRERAPYAIIGEATEERHLLLND 589

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BLAST of CK148866 vs. TrEMBL
Match: Q84XV9_SOYBN (Phosphoribosylformylglycinamidine synthase OS=Glycine max GN=FGAM2 PE=4 SV=1)

HSP 1 Score: 373.244 bits (957), Expect = 8.778e-102
Identity = 187/208 (89.90%), Postives = 191/208 (91.83%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEPLIQGFCRTFGMRLPSG+RREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRI             QNDAELDFNAVQRGDAEMAQKLYRLVR CIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVR+IVVGDHTMSVLEIWGAEYQEQDAILVKPES +LL+SI  REKVSMAVIGTISGDGRVVLVDS
Sbjct:  385 KFGEPLIQGFCRTFGMRLPSGERREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIGMGGGAASSMVSGQNDAELDFNAVQRGDAEMAQKLYRLVRACIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRAIVVGDHTMSVLEIWGAEYQEQDAILVKPESRDLLESICNREKVSMAVIGTISGDGRVVLVDS 592

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BLAST of CK148866 vs. TrEMBL
Match: Q8VYU2_VIGUN (Formylglycinamide ribonucleotide amidotransferase (Fragment) OS=Vigna unguiculata GN=pur4 PE=2 SV=1)

HSP 1 Score: 372.859 bits (956), Expect = 1.146e-101
Identity = 186/208 (89.42%), Postives = 192/208 (92.31%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEPLIQGFCRTFGMRLPSG+RREWLKPIMFSAGIGQIDHLHI+KGEPDIGMLVVKIGGPAYRI             QNDAELDFNAVQRGDAEMAQKLYRLVR CIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVR+IVVGDHTMSVLEIWGAEYQEQDAILVKPES +LL+SI +REKVSMAVIGTISGDGRVVLVDS
Sbjct:  361 KFGEPLIQGFCRTFGMRLPSGERREWLKPIMFSAGIGQIDHLHITKGEPDIGMLVVKIGGPAYRIGMGGGAASSMVSGQNDAELDFNAVQRGDAEMAQKLYRLVRACIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRAIVVGDHTMSVLEIWGAEYQEQDAILVKPESRDLLESICRREKVSMAVIGTISGDGRVVLVDS 568

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BLAST of CK148866 vs. TrEMBL
Match: Q84XV8_SOYBN (FGAM synthase OS=Glycine max GN=FGAM1 PE=4 SV=1)

HSP 1 Score: 371.703 bits (953), Expect = 2.554e-101
Identity = 186/208 (89.42%), Postives = 190/208 (91.35%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEPLIQGFCRTFGMRLP G+RREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRI             QNDAELDFNAVQRGDAEMAQKLYRLVR CIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVR+IVVGDHTMSVLEIWGAEYQEQDAILVKPES +LL+SI  REKVSMAVIGTISGDGRVVLVDS
Sbjct:  385 KFGEPLIQGFCRTFGMRLPGGERREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIGMGGGAASSMVDGQNDAELDFNAVQRGDAEMAQKLYRLVRACIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRAIVVGDHTMSVLEIWGAEYQEQDAILVKPESRDLLESICNREKVSMAVIGTISGDGRVVLVDS 592

[Close]

BLAST of CK148866 vs. TrEMBL
Match: B9HT78_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_882858 PE=4 SV=1)

HSP 1 Score: 352.058 bits (902), Expect = 2.094e-95
Identity = 173/208 (83.17%), Postives = 188/208 (90.38%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEPLIQG+ RTFGMRLPSG+RREWLKPIMFS GIGQIDH HI+KGEPDIGMLVVKIGGPAYRI             QNDA+LDFNAVQRGDAEMAQKLYR+VR+CIEMG+ NPIISIHDQGAGGNCNVVKEIIYPKGA+ID+R+IV+GDHTMSVLEIWGAEYQEQDAILVK ES +LL+SI KRE+VSMAVIGTISG+GRVVLVDS
Sbjct:  449 KFGEPLIQGYTRTFGMRLPSGERREWLKPIMFSGGIGQIDHTHITKGEPDIGMLVVKIGGPAYRIGMGGGAASSMVSGQNDADLDFNAVQRGDAEMAQKLYRVVRSCIEMGEDNPIISIHDQGAGGNCNVVKEIIYPKGAQIDIRAIVIGDHTMSVLEIWGAEYQEQDAILVKAESRDLLQSICKRERVSMAVIGTISGEGRVVLVDS 656

[Close]

BLAST of CK148866 vs. TrEMBL
Match: B9T4L5_RICCO (Phosphoribosylformylglycinamidine synthase, putative OS=Ricinus communis GN=RCOM_0246180 PE=4 SV=1)

HSP 1 Score: 350.903 bits (899), Expect = 4.665e-95
Identity = 171/208 (82.21%), Postives = 187/208 (89.90%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEPL+QG+ RTFGMRLPSG+RREWLKPIMFS GIGQIDH HI+KGEPDIGMLVVKIGGPAYRI             QNDAELDFNAVQRGDAEMAQKLYR+VRTCIEMG+ NPIISIHDQGAGGNCNVVKEIIYPKGA ID+R+IVVGDHTMS+LEIWGAEYQEQDAILVKPES +LL+SI +RE+VSMAV+G I+G+GRVVLVDS
Sbjct:  485 KFGEPLVQGYTRTFGMRLPSGERREWLKPIMFSGGIGQIDHTHITKGEPDIGMLVVKIGGPAYRIGMGGGAASSMVSGQNDAELDFNAVQRGDAEMAQKLYRVVRTCIEMGENNPIISIHDQGAGGNCNVVKEIIYPKGAVIDIRAIVVGDHTMSILEIWGAEYQEQDAILVKPESRDLLQSICERERVSMAVLGAINGEGRVVLVDS 692

[Close]

BLAST of CK148866 vs. TrEMBL
Match: B9HMK5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_563927 PE=4 SV=1)

HSP 1 Score: 350.903 bits (899), Expect = 4.665e-95
Identity = 172/208 (82.69%), Postives = 188/208 (90.38%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEPLIQG+ RTFGMRLPSG+RREWLKPIMFS GIGQIDH HI+KGEPD+GMLVVKIGGPAYRI             QNDA+LDFNAVQRGDAEMAQKLYR+VR+CIEMG+ NPIISIHDQGAGGNCNVVKEIIYPKGA+ID+++IVVGDHTMSVLEIWGAEYQEQDAILVK ES +LL+SI KRE+VSMAVIGTISG+GRVVLVDS
Sbjct:   57 KFGEPLIQGYTRTFGMRLPSGERREWLKPIMFSGGIGQIDHTHITKGEPDVGMLVVKIGGPAYRIGMGGGAASSMVSGQNDADLDFNAVQRGDAEMAQKLYRVVRSCIEMGENNPIISIHDQGAGGNCNVVKEIIYPKGAQIDIQAIVVGDHTMSVLEIWGAEYQEQDAILVKAESRDLLQSICKRERVSMAVIGTISGEGRVVLVDS 264

[Close]

BLAST of CK148866 vs. TrEMBL
Match: B9SDY9_RICCO (Phosphoribosylformylglycinamidine synthase, putative OS=Ricinus communis GN=RCOM_1480330 PE=4 SV=1)

HSP 1 Score: 349.362 bits (895), Expect = 1.357e-94
Identity = 171/208 (82.21%), Postives = 186/208 (89.42%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEPLIQG+ RTFGMRLPSG+RREWLKPIMFS GIGQIDH HI+KGEPDIGMLVVKIGGPAYRI             QNDAELDFNAVQRGDAEMAQKLYR+VR CIEMG+ NPIISIHDQGAGGNCNVVKEIIYPKGA ID+R+IVVGDHTMS+LEIWGAEYQEQDAILVKPES +LL+SI +RE+VSMAV+G I+G+GRVVLVDS
Sbjct:  486 KFGEPLIQGYTRTFGMRLPSGERREWLKPIMFSGGIGQIDHTHITKGEPDIGMLVVKIGGPAYRIGMGGGAASSMVSGQNDAELDFNAVQRGDAEMAQKLYRVVRACIEMGENNPIISIHDQGAGGNCNVVKEIIYPKGAVIDIRAIVVGDHTMSILEIWGAEYQEQDAILVKPESRDLLQSICQRERVSMAVLGAINGEGRVVLVDS 693

[Close]

BLAST of CK148866 vs. TrEMBL
Match: A9Y5J1_ARATH (Phosphoribosylformylglycinamidine synthase OS=Arabidopsis thaliana GN=pur4 PE=2 SV=1)

HSP 1 Score: 347.051 bits (889), Expect = 6.736e-94
Identity = 168/208 (80.77%), Postives = 186/208 (89.42%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEP+IQG+ RTFGMRLPSGDRREWLKPIMFSAGIGQIDH HI+KGEP++GMLVVKIGGPAYRI             QNDAELDFNAVQRGDAEM+QKLYR+VR CIEMG+KNPIISIHDQGAGGNCNVVKEIIYP+GAEID+R++VVGDHTMSVLEIWGAEYQEQDAILVK ES E+L+SI KRE++SMAVIGTI+G GR  L+DS
Sbjct:  460 KFGEPMIQGYTRTFGMRLPSGDRREWLKPIMFSAGIGQIDHTHITKGEPEVGMLVVKIGGPAYRIGMGGGAASSMVSGQNDAELDFNAVQRGDAEMSQKLYRVVRACIEMGEKNPIISIHDQGAGGNCNVVKEIIYPQGAEIDIRAVVVGDHTMSVLEIWGAEYQEQDAILVKAESREILQSICKRERLSMAVIGTINGGGRCTLIDS 667

[Close]

BLAST of CK148866 vs. TrEMBL
Match: D7KS05_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_476563 PE=4 SV=1)

HSP 1 Score: 345.895 bits (886), Expect = 1.501e-93
Identity = 167/208 (80.29%), Postives = 186/208 (89.42%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEP+IQG+ RTFGMRLPSGDRREWLKPIMFSAGIGQIDH HI+KGEP++GMLVVKIGGPAYRI             QNDAELDFNAVQRGDAEM+QKLYR+VR CIEMG+KNPIISIHDQGAGGNCNVVKEIIYP+GAEID+R++VVGDHTMSVLEIWGAEYQEQDAILVK ES E+L+SI KRE++SMA+IGTI+G GR  L+DS
Sbjct:  483 KFGEPMIQGYTRTFGMRLPSGDRREWLKPIMFSAGIGQIDHTHITKGEPEVGMLVVKIGGPAYRIGMGGGAASSMVSGQNDAELDFNAVQRGDAEMSQKLYRVVRACIEMGEKNPIISIHDQGAGGNCNVVKEIIYPQGAEIDIRAVVVGDHTMSVLEIWGAEYQEQDAILVKAESREILQSICKRERLSMAMIGTINGGGRCTLIDS 690

[Close]

BLAST of CK148866 vs. TrEMBL
Match: A9TZH0_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_172846 PE=4 SV=1)

HSP 1 Score: 341.658 bits (875), Expect = 2.830e-92
Identity = 168/208 (80.77%), Postives = 182/208 (87.50%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEPLIQG+ RTFGMRLPSG+RREWLKPIMFSAGIGQIDH H+ K +P+IGMLVVKIGGPAYRI             QN AELDFNAVQRGDAEM+QKLYR VRTC+EMGD NPI+SIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGD TMSVLEIWGAEYQEQDA+L++PES  LL+SI  RE+VSMAVIGTISGDGR+VL DS
Sbjct:  513 KFGEPLIQGYTRTFGMRLPSGERREWLKPIMFSAGIGQIDHRHLEKEDPEIGMLVVKIGGPAYRIGMGGGAASSMVSGQNTAELDFNAVQRGDAEMSQKLYRAVRTCVEMGDDNPIVSIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDETMSVLEIWGAEYQEQDALLIRPESEALLRSICARERVSMAVIGTISGDGRIVLSDS 720

[Close]

BLAST of CK148866 vs. TAIR peptide
Match: AT1G74260.1 (| Symbols: PUR4 | purine biosynthesis 4 | chr1:27923005-27927764 REVERSE LENGTH=1407)

HSP 1 Score: 347.051 bits (889), Expect = 4.812e-96
Identity = 168/208 (80.77%), Postives = 186/208 (89.42%), Query Frame = 2

Query:   14 KFGEPLIQGFCRTFGMRLPSGDRREWLKPIMFSAGIGQIDHLHISKGEPDIGMLVVKIGGPAYRIXXXXXXXXXXXXXQNDAELDFNAVQRGDAEMAQKLYRLVRTCIEMGDKNPIISIHDQGAGGNCNVVKEIIYPKGAEIDVRSIVVGDHTMSVLEIWGAEYQEQDAILVKPESYELLKSISKREKVSMAVIGTISGDGRVVLVDS 637
            KFGEP+IQG+ RTFGMRLPSGDRREWLKPIMFSAGIGQIDH HI+KGEP++GMLVVKIGGPAYRI             QNDAELDFNAVQRGDAEM+QKLYR+VR CIEMG+KNPIISIHDQGAGGNCNVVKEIIYP+GAEID+R++VVGDHTMSVLEIWGAEYQEQDAILVK ES E+L+SI KRE++SMAVIGTI+G GR  L+DS
Sbjct:  480 KFGEPMIQGYTRTFGMRLPSGDRREWLKPIMFSAGIGQIDHTHITKGEPEVGMLVVKIGGPAYRIGMGGGAASSMVSGQNDAELDFNAVQRGDAEMSQKLYRVVRACIEMGEKNPIISIHDQGAGGNCNVVKEIIYPQGAEIDIRAVVVGDHTMSVLEIWGAEYQEQDAILVKAESREILQSICKRERLSMAVIGTINGGGRCTLIDS 687

The following BLAST results are available for this feature:

BLAST of CK148866 vs. SwissProt
BLAST of CK148866 vs. TrEMBL
BLAST of CK148866 vs. TAIR peptide

BLAST of CK148866 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PUR4_ARATH	4.681e-95	80.77	Probable phosphoribosylformylglycinamidine synthas...	[more]
PUR4_DICDI	4.000e-62	55.50	Phosphoribosylformylglycinamidine synthase OS=Dict...	[more]
PUR4_MOUSE	8.631e-57	51.44	Phosphoribosylformylglycinamidine synthase OS=Mus ...	[more]
PUR4_HUMAN	2.511e-56	51.92	Phosphoribosylformylglycinamidine synthase OS=Homo...	[more]
PUR4_DROME	7.825e-50	48.11	Phosphoribosylformylglycinamidine synthase OS=Dros...	[more]
PUR4_CAEEL	2.783e-47	48.79	Probable phosphoribosylformylglycinamidine synthas...	[more]
PUR4_NEIMA	1.483e-32	35.89	Phosphoribosylformylglycinamidine synthase OS=Neis...	[more]
PUR4_HAES1	5.634e-32	35.21	Phosphoribosylformylglycinamidine synthase OS=Haem...	[more]
PUR4_NEIG1	9.609e-32	34.93	Phosphoribosylformylglycinamidine synthase OS=Neis...	[more]
PUR4_PHOLL	1.255e-31	34.43	Phosphoribosylformylglycinamidine synthase OS=Phot...	[more]

BLAST of CK148866 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q84XV9_SOYBN	8.778e-102	89.90	Phosphoribosylformylglycinamidine synthase OS=Glyc...	[more]
Q8VYU2_VIGUN	1.146e-101	89.42	Formylglycinamide ribonucleotide amidotransferase ...	[more]
Q84XV8_SOYBN	2.554e-101	89.42	FGAM synthase OS=Glycine max GN=FGAM1 PE=4 SV=1	[more]
B9HT78_POPTR	2.094e-95	83.17	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9T4L5_RICCO	4.665e-95	82.21	Phosphoribosylformylglycinamidine synthase, putati...	[more]
B9HMK5_POPTR	4.665e-95	82.69	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SDY9_RICCO	1.357e-94	82.21	Phosphoribosylformylglycinamidine synthase, putati...	[more]
A9Y5J1_ARATH	6.736e-94	80.77	Phosphoribosylformylglycinamidine synthase OS=Arab...	[more]
D7KS05_ARALY	1.501e-93	80.29	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
A9TZH0_PHYPA	2.830e-92	80.77	Predicted protein OS=Physcomitrella patens subsp. ...	[more]

BLAST of CK148866 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G74260.1	4.812e-96	80.77	\| Symbols: PUR4 \| purine biosynthesis 4 \| chr1:279...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010918	AIR synthase-related protein, C-terminal	PFAM	PF02769	AIRS_C	coord: 56..209 score: 4.3
IPR010918	AIR synthase-related protein, C-terminal	SUPERFAMILY	56042	PurM C-terminal domain-like	coord: 53..212 score: 1.6
IPR016188	PurM, N-terminal-like	SUPERFAMILY	55326	PurM N-terminal domain-like	coord: 1..52 score: 3.1
None	No IPR available	PANTHER	PTHR10099	PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE	coord: 5..212 score: 1.5
None	No IPR available	SEG	seg	seg	coord: 70..82 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK148866 ID=CK148866; Name=CK148866; organism=Cicer arietinum; type=EST; length=639bp
CGCGGCCGAGGTCAAATTCGGAGAGCCATTGATCCAAGGCTTTTGTAGAA
CTTTTGGAATGAGACTTCCTAGTGGGGATAGGCGGGAATGGTTGAAGCCT
ATCATGTTTAGTGCAGGCATTGGACAGATTGACCACCTTCATATATCAAA
GGGAGAGCCTGACATCGGAATGTTGGTTGTTAAAATTGGAGGCCCGGCTT
ATCGTATTGGAATGGGAGGCGGGGCAGCCTCAAGCATGGTTAGTGGACAG
AATGATGCAGAGCTTGATTTCAACGCTGTGCAACGTGGGGATGCCGAGAT
GGCGCAAAAGCTTTATCGTCTTGTTCGCACCTGTATTGAGATGGGGGATA
AAAATCCAATTATCAGTATTCATGATCAAGGTGCTGGTGGGAACTGCAAT
GTTGTGAAGGAAATTATATATCCAAAGGGTGCCGAGATAGATGTGCGATC
AATCGTGGTTGGTGATCATACAATGTCTGTTCTTGAGATTTGGGGCGCAG
AGTATCAGGAGCAAGATGCAATCTTGGTGAAGCCTGAAAGTTACGAGCTC
TTAAAATCAATATCTAAAAGGGAGAAGGTTTCGATGGCTGTGATTGGAAC
TATTAGTGGCGATGGTCGAGTTGTGTTGGTTGATAGTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR010918	PurM-like_C_dom
IPR016188	PurM-like_N

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