FE671642

EST Overview


Name	FE671642
Unique Name	FE671642
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	645

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FE671642 vs. SwissProt
Match: UPL4_ARATH (E3 ubiquitin-protein ligase UPL4 OS=Arabidopsis thaliana GN=UPL4 PE=1 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.782e-41
Identity = 81/99 (81.82%), Postives = 92/99 (92.93%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPP+  LL+I+ EFD +Q+RAFLQFVTG+PRLP GGLASL+PKLTIVRKH S+ +D+DLPSVMTCANYLKLPPYSSKEKMKEKL+YAITEGQGSFHLS
Sbjct: 1404 SSPPVEYLLQILHEFDREQQRAFLQFVTGSPRLPHGGLASLSPKLTIVRKHGSDSSDTDLPSVMTCANYLKLPPYSSKEKMKEKLIYAITEGQGSFHLS 1502

[Close]

BLAST of FE671642 vs. SwissProt
Match: UPL3_ARATH (E3 ubiquitin-protein ligase UPL3 OS=Arabidopsis thaliana GN=UPL3 PE=1 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.110e-34
Identity = 77/110 (70.00%), Postives = 82/110 (74.55%), Query Frame = -3

Query:  347 SPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKH------------CSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 640
            SP I+NLLEI+ E   DQ+RAF QFVTG PRLPPGGLA LNPKLTIVRKH             S  AD DLPSVMTCANYLKLPPYS+KE M +KLLYAI EGQGSF LS
Sbjct: 1779 SPAIINLLEIMGELTADQQRAFCQFVTGAPRLPPGGLAVLNPKLTIVRKHSSTSSAAANGAGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1888

[Close]

BLAST of FE671642 vs. SwissProt
Match: TRIPC_HUMAN (Probable E3 ubiquitin-protein ligase TRIP12 OS=Homo sapiens GN=TRIP12 PE=1 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 4.712e-26
Identity = 62/94 (65.96%), Postives = 69/94 (73.40%), Query Frame = -3

Query:  347 LLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRK--HCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 622
            L EI+  FD++Q+R FLQFVTG+PRLP GG  SLNP LTIVRK    +   D  LPSVMTC NYLKLP YSS E M+EKLL A  EGQ SFHLS
Sbjct: 1899 LFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS 1992

[Close]

BLAST of FE671642 vs. SwissProt
Match: UFD4_YEAST (Ubiquitin fusion degradation protein 4 OS=Saccharomyces cerevisiae GN=UFD4 PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.086e-22
Identity = 55/97 (56.70%), Postives = 68/97 (70.10%), Query Frame = -3

Query:  347 IVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSN--QADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 631
            I + + II  F   +RR FLQF+TG+P+LP GG  SLNPK T+V KH  +   AD  LPSVMTCANYLKLP Y+SK+ M+ +L  AI EG G+F LS
Sbjct: 1387 IHDFISIISAFGKHERRLFLQFLTGSPKLPIGGFKSLNPKFTVVLKHAEDGLTADEYLPSVMTCANYLKLPKYTSKDIMRSRLCQAIEEGAGAFLLS 1483

[Close]

BLAST of FE671642 vs. SwissProt
Match: YDE1_SCHPO (Probable ubiquitin fusion degradation protein C12B10.01c OS=Schizosaccharomyces pombe GN=SPAC12B10.01c PE=2 SV=2)

HSP 1 Score: 102.449 bits (254), Expect = 2.049e-21
Identity = 54/100 (54.00%), Postives = 67/100 (67.00%), Query Frame = -3

Query:  347 SPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRK--HCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 640
            SP I  LL ++ + +  ++R FLQF+TG+ +LP GG A LNP LT+VR+        D  LPSVMTC NYLKLP YSS E +  +L  AI EGQGSFHLS
Sbjct: 1548 SPTIQRLLTLMSQMNFQEQRDFLQFITGSRKLPIGGFAGLNPPLTVVRRLNEPPYVPDDYLPSVMTCVNYLKLPEYSSSEVLGSRLSKAILEGQGSFHLS 1647

[Close]

BLAST of FE671642 vs. SwissProt
Match: HECD1_MOUSE (E3 ubiquitin-protein ligase HECTD1 OS=Mus musculus GN=Hectd1 PE=1 SV=2)

HSP 1 Score: 93.5893 bits (231), Expect = 9.516e-19
Identity = 49/98 (50.00%), Postives = 66/98 (67.35%), Query Frame = -3

Query:  347 SPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 640
            SP  +  + ++     D+R+AFLQF TG   LPPGGLA+L+P+LT+VRK   +  D+  PSV TC +YLKLP YSS+E M+E+LL A  E    FHL+
Sbjct: 2525 SPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRK--VDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATME--KGFHLN 2618

[Close]

BLAST of FE671642 vs. SwissProt
Match: HECD1_HUMAN (E3 ubiquitin-protein ligase HECTD1 OS=Homo sapiens GN=HECTD1 PE=1 SV=2)

HSP 1 Score: 93.5893 bits (231), Expect = 9.516e-19
Identity = 49/98 (50.00%), Postives = 66/98 (67.35%), Query Frame = -3

Query:  347 SPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 640
            SP  +  + ++     D+R+AFLQF TG   LPPGGLA+L+P+LT+VRK   +  D+  PSV TC +YLKLP YSS+E M+E+LL A  E    FHL+
Sbjct: 2519 SPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRK--VDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATME--KGFHLN 2612

[Close]

BLAST of FE671642 vs. SwissProt
Match: UBE3A_HUMAN (Ubiquitin-protein ligase E3A OS=Homo sapiens GN=UBE3A PE=1 SV=4)

HSP 1 Score: 75.0998 bits (183), Expect = 3.502e-13
Identity = 44/86 (51.16%), Postives = 55/86 (63.95%), Query Frame = -3

Query:  362 EIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSD-LPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQG 616
            EI+  F  +Q+R FLQF TGT R P GGL  L  K+ I +    N  D++ LP+  TC N L LP YSSKEK+KE+LL AIT  +G
Sbjct:  792 EIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKL--KMIIAK----NGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKG 871

[Close]

BLAST of FE671642 vs. SwissProt
Match: WWP2_MOUSE (NEDD4-like E3 ubiquitin-protein ligase WWP2 OS=Mus musculus GN=Wwp2 PE=1 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 5.974e-13
Identity = 44/96 (45.83%), Postives = 58/96 (60.42%), Query Frame = -3

Query:  362 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSD--LPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQG 643
            SS  I    ++++E D+++R   LQFVTGT RLP GG A L       +K C ++   +  LP   TC N L LPPY S E++KEKLLYAI E +G
Sbjct:  772 SSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGS-NGPQKFCIDRVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLYAIEETEG 866

[Close]

BLAST of FE671642 vs. SwissProt
Match: YFK7_SCHPO (Probable E3 ubiquitin protein ligase C167.07c OS=Schizosaccharomyces pombe GN=SPAC167.07c PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.802e-13
Identity = 37/94 (39.36%), Postives = 58/94 (61.70%), Query Frame = -3

Query:  362 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQG 643
            +SP IV   E++REF+ + +R+F++FVT   R P  G  +L P   I     + + ++ LP+  TC N LKLP YS+K+ +++KLL A+  G G
Sbjct:  935 NSPTIVLFWEVLREFEEEDKRSFVKFVTSVARPPILGFKALMPSFCI---RVNGEDETRLPTASTCVNLLKLPMYSTKQTLRDKLLTAVRSGVG 1025

[Close]

BLAST of FE671642 vs. TrEMBL
Match: D7TQ95_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_40.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025537001 PE=4 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 1.080e-46
Identity = 90/99 (90.91%), Postives = 96/99 (96.97%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPPI+NLLEI++EFDH+QRRAFLQFVTG PRLPPGGLASLNPKLTIVRKHCS  AD+DLPSVMTCANYLKLPPYSSKE+MKEKLLYAITEGQGSFHLS
Sbjct: 1389 SSPPIINLLEIVQEFDHEQRRAFLQFVTGAPRLPPGGLASLNPKLTIVRKHCSKWADADLPSVMTCANYLKLPPYSSKERMKEKLLYAITEGQGSFHLS 1487

[Close]

BLAST of FE671642 vs. TrEMBL
Match: B9SGR6_RICCO (Ubiquitin protein ligase E3a, putative OS=Ricinus communis GN=RCOM_0819650 PE=4 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 6.551e-44
Identity = 86/99 (86.87%), Postives = 94/99 (94.95%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPPI NLLEI++ F+ +++RAFLQFVTG PRLPPGGLASLNPKLTIVRKHCSN+ D+DLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS
Sbjct: 1463 SSPPITNLLEIMQGFNQEEQRAFLQFVTGAPRLPPGGLASLNPKLTIVRKHCSNRVDADLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 1561

[Close]

BLAST of FE671642 vs. TrEMBL
Match: Q60EQ3_ORYSJ (Putative uncharacterized protein OJ1280_A04.10 OS=Oryza sativa subsp. japonica GN=OJ1280_A04.10 PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.906e-43
Identity = 83/99 (83.84%), Postives = 95/99 (95.96%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPP++NLLE+I+EF+  QRRAFLQF+TG+PRLPPGGLA+LNPKLT+VRKH SN+AD DLPSVMTCANYLKLPPYSSK+KM+EKLLYAITEGQGSFHLS
Sbjct:  425 SSPPVINLLEVIQEFEGHQRRAFLQFITGSPRLPPGGLAALNPKLTVVRKHNSNEADDDLPSVMTCANYLKLPPYSSKDKMREKLLYAITEGQGSFHLS 523

[Close]

BLAST of FE671642 vs. TrEMBL
Match: Q0DHI3_ORYSJ (Os05g0463900 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os05g0463900 PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.906e-43
Identity = 83/99 (83.84%), Postives = 95/99 (95.96%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPP++NLLE+I+EF+  QRRAFLQF+TG+PRLPPGGLA+LNPKLT+VRKH SN+AD DLPSVMTCANYLKLPPYSSK+KM+EKLLYAITEGQGSFHLS
Sbjct:  443 SSPPVINLLEVIQEFEGHQRRAFLQFITGSPRLPPGGLAALNPKLTVVRKHNSNEADDDLPSVMTCANYLKLPPYSSKDKMREKLLYAITEGQGSFHLS 541

[Close]

BLAST of FE671642 vs. TrEMBL
Match: C5YZC1_SORBI (Putative uncharacterized protein Sb09g022820 OS=Sorghum bicolor GN=Sb09g022820 PE=4 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 2.752e-42
Identity = 83/99 (83.84%), Postives = 92/99 (92.93%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPP++NLLEII+EF   +RRAFLQF+TG+PRLPPGGLA+LNPK T+VRKH SN AD DLPSVMTCANYLKLPPYSSKEKM+EKLLYAITEGQGSFHLS
Sbjct: 1416 SSPPVINLLEIIQEFGSLERRAFLQFITGSPRLPPGGLAALNPKFTVVRKHNSNDADDDLPSVMTCANYLKLPPYSSKEKMREKLLYAITEGQGSFHLS 1514

[Close]

BLAST of FE671642 vs. TrEMBL
Match: B9FKM6_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_18837 PE=4 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 4.695e-42
Identity = 83/100 (83.00%), Postives = 95/100 (95.00%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRK-HCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPP++NLLE+I+EF+  QRRAFLQF+TG+PRLPPGGLA+LNPKLT+VRK H SN+AD DLPSVMTCANYLKLPPYSSK+KM+EKLLYAITEGQGSFHLS
Sbjct:  963 SSPPVINLLEVIQEFEGHQRRAFLQFITGSPRLPPGGLAALNPKLTVVRKQHNSNEADDDLPSVMTCANYLKLPPYSSKDKMREKLLYAITEGQGSFHLS 1062

[Close]

BLAST of FE671642 vs. TrEMBL
Match: B8AZ12_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_20254 PE=4 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 4.695e-42
Identity = 83/100 (83.00%), Postives = 95/100 (95.00%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRK-HCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPP++NLLE+I+EF+  QRRAFLQF+TG+PRLPPGGLA+LNPKLT+VRK H SN+AD DLPSVMTCANYLKLPPYSSK+KM+EKLLYAITEGQGSFHLS
Sbjct: 1252 SSPPVINLLEVIQEFEGHQRRAFLQFITGSPRLPPGGLAALNPKLTVVRKQHNSNEADDDLPSVMTCANYLKLPPYSSKDKMREKLLYAITEGQGSFHLS 1351

[Close]

BLAST of FE671642 vs. TrEMBL
Match: B9MT83_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_739328 PE=4 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 2.330e-41
Identity = 87/112 (77.68%), Postives = 94/112 (83.93%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRK-------------HCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPP+VNLLEII+EF+++QRR+FLQFVTG PRLP GGLASLNPKLTIVRK             HCSN  D DLPSVMTCANYLKLPPYSSK+KMKEKLLYAITEGQGSFHLS
Sbjct:  548 SSPPVVNLLEIIKEFEYEQRRSFLQFVTGAPRLPTGGLASLNPKLTIVRKSLFNHLSSWCSLQHCSNCEDVDLPSVMTCANYLKLPPYSSKDKMKEKLLYAITEGQGSFHLS 659

[Close]

BLAST of FE671642 vs. TrEMBL
Match: D7M7X1_ARALY (Ubiquitin-protein ligase 4 OS=Arabidopsis lyrata subsp. lyrata GN=UPL4 PE=4 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 3.043e-41
Identity = 83/99 (83.84%), Postives = 92/99 (92.93%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPP+ NLLEI+ EFD +Q+RAFLQFVTG+PRLP GGLASLNPKLTIVRKH S+ +D+DLPSVMTCANYLKLP YSSKEKMKEKL+YAITEGQGSFHLS
Sbjct: 1411 SSPPVENLLEILHEFDREQQRAFLQFVTGSPRLPHGGLASLNPKLTIVRKHGSDSSDTDLPSVMTCANYLKLPAYSSKEKMKEKLIYAITEGQGSFHLS 1509

[Close]

BLAST of FE671642 vs. TrEMBL
Match: B9IIZ8_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_256737 PE=4 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 4.547e-37
Identity = 83/112 (74.11%), Postives = 89/112 (79.46%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRK-------------HCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPPIVN+     EF+++QRR+FLQFVTG PRLP GGLASLNPKLTIVRK             HCSN  D DLPSVMTCANYLKLPPYSSK+KMKEKLLYAITEGQGSFHLS
Sbjct:  544 SSPPIVNV-----EFEYEQRRSFLQFVTGAPRLPTGGLASLNPKLTIVRKSLFNHLSSWCSLQHCSNCEDVDLPSVMTCANYLKLPPYSSKDKMKEKLLYAITEGQGSFHLS 650

[Close]

BLAST of FE671642 vs. TAIR peptide
Match: AT5G02880.1 (| Symbols: UPL4 | ubiquitin-protein ligase 4 | chr5:662643-668847 FORWARD LENGTH=1502)

HSP 1 Score: 169.088 bits (427), Expect = 1.827e-42
Identity = 81/99 (81.82%), Postives = 92/99 (92.93%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SSPP+  LL+I+ EFD +Q+RAFLQFVTG+PRLP GGLASL+PKLTIVRKH S+ +D+DLPSVMTCANYLKLPPYSSKEKMKEKL+YAITEGQGSFHLS
Sbjct: 1404 SSPPVEYLLQILHEFDREQQRAFLQFVTGSPRLPHGGLASLSPKLTIVRKHGSDSSDTDLPSVMTCANYLKLPPYSSKEKMKEKLIYAITEGQGSFHLS 1502

[Close]

BLAST of FE671642 vs. TAIR peptide
Match: AT4G38600.1 (| Symbols: KAK, UPL3 | HEAT repeat ;HECT-domain (ubiquitin-transferase) | chr4:18041503-18049292 REVERSE LENGTH=1888)

HSP 1 Score: 145.591 bits (366), Expect = 2.163e-35
Identity = 77/110 (70.00%), Postives = 82/110 (74.55%), Query Frame = -3

Query:  347 SPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKH------------CSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 640
            SP I+NLLEI+ E   DQ+RAF QFVTG PRLPPGGLA LNPKLTIVRKH             S  AD DLPSVMTCANYLKLPPYS+KE M +KLLYAI EGQGSF LS
Sbjct: 1779 SPAIINLLEIMGELTADQQRAFCQFVTGAPRLPPGGLAVLNPKLTIVRKHSSTSSAAANGAGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1888

[Close]

BLAST of FE671642 vs. TAIR peptide
Match: AT4G38600.2 (| Symbols: KAK, UPL3 | HEAT repeat ;HECT-domain (ubiquitin-transferase) | chr4:18041503-18049073 REVERSE LENGTH=1794)

HSP 1 Score: 135.191 bits (339), Expect = 2.923e-32
Identity = 74/110 (67.27%), Postives = 79/110 (71.82%), Query Frame = -3

Query:  347 SPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSNQ------------ADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 640
            SP I+N   I+ E   DQ+RAF QFVTG PRLPPGGLA LNPKLTIVRKH S              AD DLPSVMTCANYLKLPPYS+KE M +KLLYAI EGQGSF LS
Sbjct: 1688 SPAIIN---IMGELTADQQRAFCQFVTGAPRLPPGGLAVLNPKLTIVRKHSSTSSAAANGAGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1794

[Close]

BLAST of FE671642 vs. TAIR peptide
Match: AT3G17205.3 (| Symbols: UPL6 | ubiquitin protein ligase 6 | chr3:5873528-5881132 FORWARD LENGTH=1029)

HSP 1 Score: 68.9366 bits (167), Expect = 2.573e-12
Identity = 39/92 (42.39%), Postives = 54/92 (58.70%), Query Frame = -3

Query:  347 EIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRK--HCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 616
            E+++ F  + ++ FL+FVTG  R P  G   L P   I R     SN++   LP+  TC N LKLPPY SKE ++ KL+YAI+  +  F LS
Sbjct:  939 EVMKSFSTENQKKFLKFVTGCSRGPLLGFKYLEPAFCIQRAAGSASNESVDRLPTSATCMNLLKLPPYQSKELLETKLMYAIS-AEAGFDLS 1029

[Close]

BLAST of FE671642 vs. TAIR peptide
Match: AT3G17205.2 (| Symbols: UPL6 | ubiquitin protein ligase 6 | chr3:5873528-5881132 FORWARD LENGTH=1015)

HSP 1 Score: 68.9366 bits (167), Expect = 2.573e-12
Identity = 39/92 (42.39%), Postives = 54/92 (58.70%), Query Frame = -3

Query:  347 EIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRK--HCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 616
            E+++ F  + ++ FL+FVTG  R P  G   L P   I R     SN++   LP+  TC N LKLPPY SKE ++ KL+YAI+  +  F LS
Sbjct:  925 EVMKSFSTENQKKFLKFVTGCSRGPLLGFKYLEPAFCIQRAAGSASNESVDRLPTSATCMNLLKLPPYQSKELLETKLMYAIS-AEAGFDLS 1015

[Close]

BLAST of FE671642 vs. TAIR peptide
Match: AT3G17205.1 (| Symbols: UPL6 | ubiquitin protein ligase 6 | chr3:5873528-5881132 FORWARD LENGTH=1029)

HSP 1 Score: 68.9366 bits (167), Expect = 2.573e-12
Identity = 39/92 (42.39%), Postives = 54/92 (58.70%), Query Frame = -3

Query:  347 EIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRK--HCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 616
            E+++ F  + ++ FL+FVTG  R P  G   L P   I R     SN++   LP+  TC N LKLPPY SKE ++ KL+YAI+  +  F LS
Sbjct:  939 EVMKSFSTENQKKFLKFVTGCSRGPLLGFKYLEPAFCIQRAAGSASNESVDRLPTSATCMNLLKLPPYQSKELLETKLMYAIS-AEAGFDLS 1029

[Close]

BLAST of FE671642 vs. TAIR peptide
Match: AT1G55860.2 (| Symbols: UPL1 | ubiquitin-protein ligase 1 | chr1:20879900-20892910 REVERSE LENGTH=3681)

HSP 1 Score: 63.5438 bits (153), Expect = 1.081e-10
Identity = 40/100 (40.00%), Postives = 53/100 (53.00%), Query Frame = -3

Query:  356 SPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLN-----PKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSF 640
            SP I    E+++ F  +    FLQFVTGT ++P  G  +L       +L I   H +  A   LPS  TC N L LP Y SKE+++E+LL AI E    F
Sbjct: 3582 SPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRLQI---HKAYGAPERLPSAHTCFNQLDLPEYQSKEQLQERLLLAIHEASEGF 3678

[Close]

BLAST of FE671642 vs. TAIR peptide
Match: AT1G55860.1 (| Symbols: UPL1 | ubiquitin-protein ligase 1 | chr1:20879900-20895393 REVERSE LENGTH=3930)

HSP 1 Score: 63.5438 bits (153), Expect = 1.081e-10
Identity = 40/100 (40.00%), Postives = 53/100 (53.00%), Query Frame = -3

Query:  356 SPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLN-----PKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSF 640
            SP I    E+++ F  +    FLQFVTGT ++P  G  +L       +L I   H +  A   LPS  TC N L LP Y SKE+++E+LL AI E    F
Sbjct: 3831 SPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRLQI---HKAYGAPERLPSAHTCFNQLDLPEYQSKEQLQERLLLAIHEASEGF 3927

[Close]

BLAST of FE671642 vs. TAIR peptide
Match: AT1G70320.1 (| Symbols: UPL2 | ubiquitin-protein ligase 2 | chr1:26488745-26501281 REVERSE LENGTH=3658)

HSP 1 Score: 62.003 bits (149), Expect = 3.145e-10
Identity = 39/100 (39.00%), Postives = 54/100 (54.00%), Query Frame = -3

Query:  356 SPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLN-----PKLTIVRKHCSNQADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSF 640
            SP I    E+++ F  +    FLQFVTGT ++P  G  +L       +L I + + S +    LPS  TC N L LP Y SKE+++E+LL AI E    F
Sbjct: 3559 SPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRLQIHKAYGSPER---LPSAHTCFNQLDLPEYQSKEQVQERLLLAIHEANEGF 3655

[Close]

BLAST of FE671642 vs. TAIR peptide
Match: AT3G53090.2 (| Symbols: UPL7 | ubiquitin-protein ligase 7 | chr3:19679273-19684453 FORWARD LENGTH=1142)

HSP 1 Score: 61.2326 bits (147), Expect = 5.365e-10
Identity = 42/107 (39.25%), Postives = 50/107 (46.73%), Query Frame = -3

Query:  347 SSPPIVNLLEIIREFDHDQRRAFLQFVTGTPRLPPGGLASLNPKLTIVRKHCSN--------QADSDLPSVMTCANYLKLPPYSSKEKMKEKLLYAITEGQGSFHLS 643
            SS  I    E+++ F+  +R   L+FVT   R P  G   L P   I +  C          Q    LPS  TC N LKLP Y     M+EKLLYAIT   G F LS
Sbjct: 1037 SSRTIKIFWEVMKGFEPSERCLLLKFVTSCSRAPLLGFKYLQPTFIIHKVSCDTSLWAAIGGQDVERLPSASTCYNTLKLPTYKRASTMREKLLYAITSNAG-FELS 1142

The following BLAST results are available for this feature:

BLAST of FE671642 vs. SwissProt
BLAST of FE671642 vs. TrEMBL
BLAST of FE671642 vs. TAIR peptide

BLAST of FE671642 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
UPL4_ARATH	1.782e-41	81.82	E3 ubiquitin-protein ligase UPL4 OS=Arabidopsis th...	[more]
UPL3_ARATH	2.110e-34	70.00	E3 ubiquitin-protein ligase UPL3 OS=Arabidopsis th...	[more]
TRIPC_HUMAN	4.712e-26	65.96	Probable E3 ubiquitin-protein ligase TRIP12 OS=Hom...	[more]
UFD4_YEAST	1.086e-22	56.70	Ubiquitin fusion degradation protein 4 OS=Saccharo...	[more]
YDE1_SCHPO	2.049e-21	54.00	Probable ubiquitin fusion degradation protein C12B...	[more]
HECD1_MOUSE	9.516e-19	50.00	E3 ubiquitin-protein ligase HECTD1 OS=Mus musculus...	[more]
HECD1_HUMAN	9.516e-19	50.00	E3 ubiquitin-protein ligase HECTD1 OS=Homo sapiens...	[more]
UBE3A_HUMAN	3.502e-13	51.16	Ubiquitin-protein ligase E3A OS=Homo sapiens GN=UB...	[more]
WWP2_MOUSE	5.974e-13	45.83	NEDD4-like E3 ubiquitin-protein ligase WWP2 OS=Mus...	[more]
YFK7_SCHPO	7.802e-13	39.36	Probable E3 ubiquitin protein ligase C167.07c OS=S...	[more]

BLAST of FE671642 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7TQ95_VITVI	1.080e-46	90.91	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SGR6_RICCO	6.551e-44	86.87	Ubiquitin protein ligase E3a, putative OS=Ricinus ...	[more]
Q60EQ3_ORYSJ	1.906e-43	83.84	Putative uncharacterized protein OJ1280_A04.10 OS=...	[more]
Q0DHI3_ORYSJ	1.906e-43	83.84	Os05g0463900 protein (Fragment) OS=Oryza sativa su...	[more]
C5YZC1_SORBI	2.752e-42	83.84	Putative uncharacterized protein Sb09g022820 OS=So...	[more]
B9FKM6_ORYSJ	4.695e-42	83.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8AZ12_ORYSI	4.695e-42	83.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B9MT83_POPTR	2.330e-41	77.68	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7M7X1_ARALY	3.043e-41	83.84	Ubiquitin-protein ligase 4 OS=Arabidopsis lyrata s...	[more]
B9IIZ8_POPTR	4.547e-37	74.11	Predicted protein (Fragment) OS=Populus trichocarp...	[more]

BLAST of FE671642 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G02880.1	1.827e-42	81.82	\| Symbols: UPL4 \| ubiquitin-protein ligase 4 \| chr...	[more]
AT4G38600.1	2.163e-35	70.00	\| Symbols: KAK, UPL3 \| HEAT repeat ;HECT-domain (u...	[more]
AT4G38600.2	2.923e-32	67.27	\| Symbols: KAK, UPL3 \| HEAT repeat ;HECT-domain (u...	[more]
AT3G17205.3	2.573e-12	42.39	\| Symbols: UPL6 \| ubiquitin protein ligase 6 \| chr...	[more]
AT3G17205.2	2.573e-12	42.39	\| Symbols: UPL6 \| ubiquitin protein ligase 6 \| chr...	[more]
AT3G17205.1	2.573e-12	42.39	\| Symbols: UPL6 \| ubiquitin protein ligase 6 \| chr...	[more]
AT1G55860.2	1.081e-10	40.00	\| Symbols: UPL1 \| ubiquitin-protein ligase 1 \| chr...	[more]
AT1G55860.1	1.081e-10	40.00	\| Symbols: UPL1 \| ubiquitin-protein ligase 1 \| chr...	[more]
AT1G70320.1	3.145e-10	39.00	\| Symbols: UPL2 \| ubiquitin-protein ligase 2 \| chr...	[more]
AT3G53090.2	5.365e-10	39.25	\| Symbols: UPL7 \| ubiquitin-protein ligase 7 \| chr...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000569	HECT	PFAM	PF00632	HECT	coord: 2..99 score: 2.5
IPR000569	HECT	PROFILE	PS50237	HECT	coord: 1..99 score: 29
IPR000569	HECT	SUPERFAMILY	56204	Hect, E3 ligase catalytic domain	coord: 1..92 score: 1.4
None	No IPR available	PANTHER	PTHR11254	HECT DOMAIN UBIQUITIN-PROTEIN LIGASE	coord: 1..91 score: 4.5
None	No IPR available	PANTHER	PTHR11254:SF73	HECT UBIQUITIN-PROTEIN LIGASE 3 (KAKTUS PROTEIN)	coord: 1..91 score: 4.5

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE671642 ID=FE671642; Name=FE671642; organism=Cicer arietinum; type=EST; length=645bp
GGGCAGCAAAGATAGGAAACTACTATTTTCACTGCATGTAAACGGATATT
AATCGTTAAGGTCCCTATACAAAATACTGTACAAAAAATATCAAAGCATG
ACTACTTAACTTGCCCTCCGTTTCTATACAACGGACACAAGCCACCAATA
AACAGCTAAACTATCAGCAGGCTATATACACCCCCCAACAATTGTAGTCA
GTGAAAACATGAGCTTTGTCCCCAAGTAACTTCTTGCGACCAAAACTTCC
AGCAGCTAGTTAACACCCTTATCTACATTGCTATAGTAACCCTAATAATT
ACACCTTCAAAATCCAAATACAGATCCAGTCAAGAGAATGAAGTCATGAA
AGGTGGAAAGATCCTTGACCCTCTGTTATGGCATACAAGAGCTTCTCTTT
CATCTTTTCTTTTGATGAATAAGGAGGTAGCTTAAGGTAATTTGCACATG
TCATCACACTAGGTAGGTCTGAGTCTGCTTGGTTGCTACAATGCTTTCGG
ACGATAGTCAACTTTGGATTGAGAGATGCCAATCCTCCTGGAGGAAGCCG
AGGTGTGCCAGTCACGAACTGCAGAAATGCTCGTCTCTGGTCGTGGTCAA
ACTCTCGGATAATTTCCAACAAGTTAACAATTGGAGGACTGCTAC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular anatomical structure

Vocabulary: **Biological Process**
Term	Definition
GO:0006464	cellular protein modification process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016881	acid-amino acid ligase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000569	HECT_dom

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