CK148704

EST Overview


Name	CK148704
Unique Name	CK148704
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	683

Libraries


Library Name	Type
LIBEST_014674 Suppressive subtractive hybridization library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of CK148704 vs. TrEMBL
Match: B9SGT8_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0820170 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.215e-29
Identity = 50/89 (56.18%), Postives = 63/89 (70.79%), Query Frame = 3

Query:  111 PTAANSTDENPNKTLDHPTTSPKPHSD--PISADIDTGADKSKDTPADLENDAPLSDVQKKMRRAERFGISVQLSEKEKRNSRAERFGT 371
            PTA    ++NP+K LD P  SP   SD   ++A ++  A+ SK T     N AP+SD +KK+RRAERFGI+VQLSE+EKRNSRAERFGT
Sbjct:    6 PTAIAPLEDNPSKALDAPIPSPV-QSDLVEVTAPVNKDAEDSKSTKVS-GNTAPVSDTEKKIRRAERFGITVQLSEQEKRNSRAERFGT 92

HSP 2 Score: 65.4698 bits (158), Expect = 2.215e-29
Identity = 38/64 (59.38%), Postives = 45/64 (70.31%), Query Frame = 1

Query:  367 AQRVLHGPEGSKDEDLKRKARAERFGMPTPSTTADEDAKKKARLARFASGSKTDPAEEDKRKAR 558
            A+R   GP     E+LKRKARAERFG+P  + +ADE+AKKKARL RFA  SK    EEDK KA+
Sbjct:   87 AERFGTGPTVKSGEELKRKARAERFGLPV-AVSADEEAKKKARLQRFAPTSK---EEEDKEKAK 146

[Close]

BLAST of CK148704 vs. TrEMBL
Match: B9IIY1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_667725 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 2.216e-29
Identity = 47/107 (43.93%), Postives = 70/107 (65.42%), Query Frame = 3

Query:  126 STDENPNKTLDHPTT-SPKPH-----SDPISADIDTGADKSKDTPADLENDAPLSDVQKKMRRAERFGISVQLSEKEKRNSRAERFGTACITWT-RGIKR*GSEKES 425
            + ++NP++TLD     S KP      ++  S+ ++   +  K    + ++ AP++D++KK+RRAERFGI+VQLSE+EKRNSRAERFGT+  T T  G    GSE +S
Sbjct:   12 AVEDNPSETLDTAVLDSTKPDRGEDSTNTASSTVELATEGPKKDSENSKSAAPVTDIEKKIRRAERFGITVQLSEQEKRNSRAERFGTSTGTGTGTGSTAQGSESDS 118

HSP 2 Score: 74.7146 bits (182), Expect = 2.216e-29
Identity = 34/57 (59.65%), Postives = 45/57 (78.95%), Query Frame = 1

Query:  400 KDEDLKRKARAERFGMPTPSTTADEDAKKKARLARFASGSKTDPAEEDKRKARALSF 570
            K E+LKRKARAERFG+P P   +DE+AKKK+RL +F   +K++  E+DKRKARAL +
Sbjct:  121 KSEELKRKARAERFGIPVPPAASDEEAKKKSRLEKFTPAAKSNTLEDDKRKARALRY 177

[Close]

BLAST of CK148704 vs. TrEMBL
Match: B9MT62_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_675939 PE=4 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 4.878e-29
Identity = 47/102 (46.08%), Postives = 63/102 (61.76%), Query Frame = 3

Query:   84 TATGRNGHTPTAANSTDENPNKTLDHPT-TSPKPH-----SDPISADIDTGADKSKDTPADLENDAPLSDVQKKMRRAERFGISVQLSEKEKRNSRAERFGT 371
            T T        AA + ++NP+KTLD     S KP      ++  S+ +D+  + S     + ++ AP +D  KK+RRAERFGI+VQLSEKEKRNSRAERFGT
Sbjct:    3 TETENPSTASAAALTVEDNPSKTLDTAVPASTKPDRVEDSTNTASSTVDSATEGSMQGADNSKSAAPATDTDKKIRRAERFGITVQLSEKEKRNSRAERFGT 104

HSP 2 Score: 72.4034 bits (176), Expect = 4.878e-29
Identity = 39/67 (58.21%), Postives = 43/67 (64.18%), Query Frame = 1

Query:  385 GPEGSKD-----EDLKRKARAERFGMPTPSTTADEDAKKKARLARFASGSKTDPAEEDKRKARALSF 570
            GPE   D     E+LKRKARAERFG+P P T   E+ KKKAR  R A   K D  EE+KRKARAL F
Sbjct:  108 GPESESDSVKKSEELKRKARAERFGIPVPPTATAEETKKKARHERLAPAPKIDALEENKRKARALRF 174

[Close]

BLAST of CK148704 vs. TrEMBL
Match: B6TDH3_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.046e-21
Identity = 37/68 (54.41%), Postives = 49/68 (72.06%), Query Frame = 1

Query:  367 AQRVLHGPEGSKDEDLKRKARAERFGMPTPSTTADEDAKKKARLARFASGSKTDPAEEDKRKARALSF 570
            A+R   G    K+E+ K+K+RAERFG+ +PS++ DE+AKKKARL RF   +  D AEE+KRKARA  F
Sbjct:   76 AERFGTGSSSVKEEE-KKKSRAERFGLASPSSS-DEEAKKKARLERFGQSANVDKAEEEKRKARAARF 141

HSP 2 Score: 60.077 bits (144), Expect = 1.046e-21
Identity = 37/81 (45.68%), Postives = 50/81 (61.73%), Query Frame = 3

Query:  144 NKTLDHPTTSPKPHSDPISADIDTGADKSKDTPADLENDAPLS-----DVQKKMRRAERFGISVQLSEKEKRNSRAERFGT 371
            +KT D  T +P P    ++A  +     +  TPA   N A  +     D++KKMRRAERFG+ V +SE+EKR+SRAERFGT
Sbjct:    6 SKTAD--TAAPPPE---VAATGEAAPGAASSTPAQNPNAAATAVAGATDLEKKMRRAERFGMPVLMSEEEKRSSRAERFGT 81

[Close]

BLAST of CK148704 vs. TrEMBL
Match: A3AGQ8_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_10389 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.766e-21
Identity = 39/68 (57.35%), Postives = 47/68 (69.12%), Query Frame = 1

Query:  367 AQRVLHGPEGSKDEDLKRKARAERFGMPTPSTTADEDAKKKARLARFASGSKTDPAEEDKRKARALSF 570
            A+R   G    K E+ KRK+RAERFG+   S++ADEDAKKKARL RF   +  D  EE+KRKARAL F
Sbjct:   83 AERFGTGSSNEKAEEQKRKSRAERFGLA--SSSADEDAKKKARLERFGQSTNVDKGEEEKRKARALRF 148

HSP 2 Score: 55.0694 bits (131), Expect = 1.766e-21
Identity = 25/34 (73.53%), Postives = 32/34 (94.12%), Query Frame = 3

Query:  270 SDVQKKMRRAERFGISVQLSEKEKRNSRAERFGT 371
            +D++KKMRRAERFG +V +SE+EKR+SRAERFGT
Sbjct:   55 TDLEKKMRRAERFGTAVVMSEEEKRSSRAERFGT 88

[Close]

BLAST of CK148704 vs. TrEMBL
Match: A2XFB1_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_11054 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.766e-21
Identity = 39/68 (57.35%), Postives = 47/68 (69.12%), Query Frame = 1

Query:  367 AQRVLHGPEGSKDEDLKRKARAERFGMPTPSTTADEDAKKKARLARFASGSKTDPAEEDKRKARALSF 570
            A+R   G    K E+ KRK+RAERFG+   S++ADEDAKKKARL RF   +  D  EE+KRKARAL F
Sbjct:   83 AERFGTGSSNEKAEEQKRKSRAERFGLA--SSSADEDAKKKARLERFGQSTNVDKGEEEKRKARALRF 148

HSP 2 Score: 55.0694 bits (131), Expect = 1.766e-21
Identity = 25/34 (73.53%), Postives = 32/34 (94.12%), Query Frame = 3

Query:  270 SDVQKKMRRAERFGISVQLSEKEKRNSRAERFGT 371
            +D++KKMRRAERFG +V +SE+EKR+SRAERFGT
Sbjct:   55 TDLEKKMRRAERFGTAVVMSEEEKRSSRAERFGT 88

[Close]

BLAST of CK148704 vs. TrEMBL
Match: Q8H8U3_ORYSJ (Expressed protein OS=Oryza sativa subsp. japonica GN=Os03g0283300 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.766e-21
Identity = 39/68 (57.35%), Postives = 47/68 (69.12%), Query Frame = 1

Query:  367 AQRVLHGPEGSKDEDLKRKARAERFGMPTPSTTADEDAKKKARLARFASGSKTDPAEEDKRKARALSF 570
            A+R   G    K E+ KRK+RAERFG+   S++ADEDAKKKARL RF   +  D  EE+KRKARAL F
Sbjct:   83 AERFGTGSSNEKAEEQKRKSRAERFGLA--SSSADEDAKKKARLERFGQSTNVDKGEEEKRKARALRF 148

HSP 2 Score: 55.0694 bits (131), Expect = 1.766e-21
Identity = 25/34 (73.53%), Postives = 32/34 (94.12%), Query Frame = 3

Query:  270 SDVQKKMRRAERFGISVQLSEKEKRNSRAERFGT 371
            +D++KKMRRAERFG +V +SE+EKR+SRAERFGT
Sbjct:   55 TDLEKKMRRAERFGTAVVMSEEEKRSSRAERFGT 88

[Close]

BLAST of CK148704 vs. TrEMBL
Match: Q10N42_ORYSJ (Expressed protein OS=Oryza sativa subsp. japonica GN=LOC_Os03g17490 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.767e-21
Identity = 39/68 (57.35%), Postives = 47/68 (69.12%), Query Frame = 1

Query:  367 AQRVLHGPEGSKDEDLKRKARAERFGMPTPSTTADEDAKKKARLARFASGSKTDPAEEDKRKARALSF 570
            A+R   G    K E+ KRK+RAERFG+   S++ADEDAKKKARL RF   +  D  EE+KRKARAL F
Sbjct:   83 AERFGTGSSNEKAEEQKRKSRAERFGLA--SSSADEDAKKKARLERFGQSTNVDKGEEEKRKARALRF 148

HSP 2 Score: 55.0694 bits (131), Expect = 1.767e-21
Identity = 25/34 (73.53%), Postives = 32/34 (94.12%), Query Frame = 3

Query:  270 SDVQKKMRRAERFGISVQLSEKEKRNSRAERFGT 371
            +D++KKMRRAERFG +V +SE+EKR+SRAERFGT
Sbjct:   55 TDLEKKMRRAERFGTAVVMSEEEKRSSRAERFGT 88

[Close]

BLAST of CK148704 vs. TrEMBL
Match: D7TQC0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_40.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025570001 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 6.944e-21
Identity = 45/65 (69.23%), Postives = 53/65 (81.54%), Query Frame = 1

Query:  379 LHGPEGSK-DEDLKRKARAERFGMPTPSTTADEDAKKKARLARFASGSKTDPAEEDKRKARALSF 570
            +HG +GSK  E+LKRKARAERFG+P  S   DE++KKKARLARFAS SKTD  EEDK+KARA+ F
Sbjct:   24 VHGSDGSKKSEELKRKARAERFGLPLDSAPTDEESKKKARLARFASVSKTDTLEEDKKKARAIRF 88

HSP 2 Score: 36.965 bits (84), Expect = 6.944e-21
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 3

Query:  318 VQLSEKEKRNSRAERFGT 371
            VQLSE+EKRNSRAERFGT
Sbjct:    3 VQLSEEEKRNSRAERFGT 20

[Close]

BLAST of CK148704 vs. TrEMBL
Match: C5WP84_SORBI (Putative uncharacterized protein Sb01g038880 OS=Sorghum bicolor GN=Sb01g038880 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.202e-20
Identity = 37/68 (54.41%), Postives = 50/68 (73.53%), Query Frame = 1

Query:  367 AQRVLHGPEGSKDEDLKRKARAERFGMPTPSTTADEDAKKKARLARFASGSKTDPAEEDKRKARALSF 570
            A+R   G    K+E+ K+K+RAERFG+ + S+++DE+AKKKARL RF  G+  D AEE+KRKARA  F
Sbjct:   78 AERFGTGSSSVKEEE-KKKSRAERFGLAS-SSSSDEEAKKKARLERFGQGTNVDKAEEEKRKARAARF 143

HSP 2 Score: 55.0694 bits (131), Expect = 3.202e-20
Identity = 32/74 (43.24%), Postives = 44/74 (59.46%), Query Frame = 3

Query:  165 TTSPKPHSDPISADIDTGADKSKDTPADLENDAPLS-----DVQKKMRRAERFGISVQLSEKEKRNSRAERFGT 371
            T +P P  +  +A  +     +  TPA     A  +     D++KKMRRAERFG  V +SE+EKR++RAERFGT
Sbjct:   11 TAAPAPAPEAAAAG-EAAPSAASSTPAQNPTAAATAAAGATDLEKKMRRAERFGTPVVMSEEEKRSNRAERFGT 83

[Close]

BLAST of CK148704 vs. TAIR peptide
Match: AT5G02770.1 (| Symbols: | unknown protein; Has 469 Blast hits to 336 proteins in 126 species: Archae - 0; Bacteria - 54; Metazoa - 249; Fungi - 22; Plants - 47; Viruses - 0; Other Eukaryotes - 97 (source: NCBI BLink). | chr5:628101-629168 REVERSE LENGTH=214)

HSP 1 Score: 81.2629 bits (199), Expect = 5.502e-16
Identity = 50/100 (50.00%), Postives = 65/100 (65.00%), Query Frame = 1

Query:  283 RRCVALNDSVFPSSCPKKRSAILEPKDLAQRVLHGPEGSKD-EDLKRKARAERFGMPTPSTTAD---EDAKKKARLARFASGSKTDPAEEDKRKARALSF 570
            RR      SV  +   K+ S       +A  V++G EG+K  E+LKRKARA+RFG+P+ ++T D   E+AKKKARLARF   +K D AEE+KRKARAL F
Sbjct:   86 RRAERFGVSVKLTEEEKRNSRAERFGTVAAAVVNGSEGTKKAEELKRKARADRFGVPSATSTTDKTEEEAKKKARLARFGKETKVDSAEENKRKARALRF 185

HSP 2 Score: 75.485 bits (184), Expect = 3.019e-14
Identity = 51/126 (40.48%), Postives = 72/126 (57.14%), Query Frame = 3

Query:   87 ATGRNGHTPTAAN----STDENPNKTLDHPTTSPKPHSDPISADI----DTGADKSKDTPA---------DLENDAPLSDVQKKMRRAERFGISVQLSEKEKRNSRAERFGT---ACITWTRGIKR 404
            AT     T T  N    ST ENP K +D  TT      D +  ++    D+G +K ++T +         D  + +P+ D+QKK+RRAERFG+SV+L+E+EKRNSRAERFGT   A +  + G K+
Sbjct:    2 ATNGEKVTATVVNGGGLSTGENPKKIVDLNTTELDRTDDILDGEVKGFSDSG-EKKEETDSNGIGSTAGVDSGDISPVDDIQKKIRRAERFGVSVKLTEEEKRNSRAERFGTVAAAVVNGSEGTKK 126

The following BLAST results are available for this feature:

BLAST of CK148704 vs. TrEMBL
BLAST of CK148704 vs. TAIR peptide

BLAST of CK148704 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SGT8_RICCO	2.215e-29	56.18	Putative uncharacterized protein OS=Ricinus commun...	[more]
B9IIY1_POPTR	2.216e-29	43.93	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9MT62_POPTR	4.878e-29	46.08	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B6TDH3_MAIZE	1.046e-21	54.41	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
A3AGQ8_ORYSJ	1.766e-21	57.35	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A2XFB1_ORYSI	1.766e-21	57.35	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q8H8U3_ORYSJ	1.766e-21	57.35	Expressed protein OS=Oryza sativa subsp. japonica ...	[more]
Q10N42_ORYSJ	1.767e-21	57.35	Expressed protein OS=Oryza sativa subsp. japonica ...	[more]
D7TQC0_VITVI	6.944e-21	69.23	Whole genome shotgun sequence of line PN40024, sca...	[more]
C5WP84_SORBI	3.202e-20	54.41	Putative uncharacterized protein Sb01g038880 OS=So...	[more]

BLAST of CK148704 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G02770.1	5.502e-16	50.00	\| Symbols: \| unknown protein; Has 469 Blast hits ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 4..15 score: NAcoord: 23..39 score
None	No IPR available	SEG	seg	seg	coord: 2..13 score: NAcoord: 27..35 score: NAcoord: 45..57 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK148704 ID=CK148704; Name=CK148704; organism=Cicer arietinum; type=EST; length=683bp
GGGCGAATTGCAATTTAGACGGCGCGGAATTGCGCCCTTTAACGGGGGGC
AGGTCTCGTAGGCTCGGTCTAAACGTTCTTCACACTGCCACCGGGCGAAA
TGGACATACGCCCACCGCCGCTAACTCAACAGACGAAAACCCTAACAAAA
CCCTAGACCACCCCACCACCTCACCCAAACCACACTCAGATCCAATCTCC
GCCGACATTGACACGGGCGCCGACAAATCGAAGGACACTCCGGCCGATCT
CGAAAACGACGCTCCACTTTCCGATGTTCAGAAGAAGATGCGTCGCGCTG
AACGATTCGGTATTTCCGTCCAGTTGTCCGAAAAAGAGAAGCGCAATTCT
CGAGCCGAAAGATTTGGCACAGCGTGTATTACATGGACCCGAGGGATCAA
AAGATGAGGATCTGAAAAGGAAAGCTAGGGCTGAGAGATTTGGAATGCCT
ACTCCTTCTACGACCGCCGACGAGGATGCAAAGAAGAAAGCTCGTCTTGC
TAGGTTTGCATCTGGTTCGAAGACTGACCCTGCAGAGGAAGATAAAAGGA
AAGCGAGAGCACTTAGTTTTCAAATTCATCATCAACGACTCTGTCCAAGT
CAATGTCGAACAAACATTGACCTGCTGCATTGCAGCAATGCTGGAGAGGC
ACCTGATTTTGCCTTGCTTCTTTTTGGAGGATT

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