FE672165

EST Overview


Name	FE672165
Unique Name	FE672165
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	596

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FE672165 vs. SwissProt
Match: IDNK_ECOLI (Thermosensitive gluconokinase OS=Escherichia coli (strain K12) GN=idnK PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.650e-27
Identity = 69/157 (43.95%), Postives = 95/157 (60.51%), Query Frame = 2

Query:   98 VIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDA-IKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNID--DSERIFRVD 559
            ++MGVSG+GKT IG ++   +  K++D DD H   N +KMS GIPL+DEDR PWLE L DA    Y  N+ G ++ CS+LKKQYR+ILR   P          V+F+ LD   E ++ R+  RA    H+MP +LL+SQ + L     D + I R+D
Sbjct:    7 ILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNIDKMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIV-CSSLKKQYRDILRKGSPH---------VHFLWLDGDYETILARMQRRAG---HFMPVALLKSQFEALERPQADEQDIVRID 150

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BLAST of FE672165 vs. SwissProt
Match: GNTK_XENTR (Probable gluconokinase OS=Xenopus tropicalis PE=2 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 2.003e-25
Identity = 69/175 (39.43%), Postives = 96/175 (54.86%), Query Frame = 2

Query:   92 VLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESY------ASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDS-ERIFRVDATLSPESIVNTI 595
            ++VIMGVSG+GKT +G +L K + + + DADD+H   NKEKMS G PL D+DR PWL  L + +       + +VL CSALK+ YR  L +    +  E+Y      +S   F+ L    E+L  RL  R     H+MP +LL SQ+D L    + ER   +D       IV+ I
Sbjct:    2 IIVIMGVSGSGKTVVGSQLAKKLGWNFYDADDYHPLENKEKMSQGTPLNDQDRHPWLCELHEIMMREKALGQHVVLACSALKRAYRSTLLTGSTPHWPENYQENDDLSSDTLFVHLHGSLEILSRRLLERKG---HFMPRTLLDSQIDTLEPPSAPERFIAIDVDKDISVIVSEI 173

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BLAST of FE672165 vs. SwissProt
Match: GNTK_RAT (Probable gluconokinase OS=Rattus norvegicus PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.298e-24
Identity = 64/153 (41.83%), Postives = 91/153 (59.48%), Query Frame = 2

Query:   92 VLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASAV-------NFILLDV--PAEVLITRLNNRAAEGEHYMPASLLQSQLDLL 523
            VL++MGVSG+GK+T+G  L   + +K+ DADD+HS+ N+ KM  G+PL D+DR PWL SL D +   + + + +VL CSALKK YR+IL     D    S  SA         F+++ +    E++  RL  R     H+MP  LLQSQ  +L
Sbjct:    6 VLLVMGVSGSGKSTVGALLANKLGWKFYDADDYHSEENRIKMGKGVPLNDQDRIPWLCSLHDILLRDVASGQSVVLACSALKKMYRDILNRGGSDVPPRSDESAKEEPLAGGKFLVVHLCGSFELIYGRLLQRRG---HFMPPELLQSQFSIL 155

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BLAST of FE672165 vs. SwissProt
Match: GNTK_MOUSE (Probable gluconokinase OS=Mus musculus PE=2 SV=2)

HSP 1 Score: 108.997 bits (271), Expect = 1.875e-23
Identity = 62/152 (40.79%), Postives = 91/152 (59.87%), Query Frame = 2

Query:   92 VLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASA--------VNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLL 523
            VL++MGVSG+GK+T+G  L   + +K+ DADD+HS+ N+ KM+ G+PL+D+DR PWL +L D +   +   + +VL CSALKK YR+IL     D   +S  SA        +  + L    +++  RL  R     H+MP  LLQSQ  +L
Sbjct:    6 VLLVMGVSGSGKSTVGALLASKLGWKFYDADDYHSEENRIKMAKGVPLSDQDRIPWLCTLHDILLRDVALGQPVVLACSALKKTYRDILIRGGSDAPLKSDDSAKEPLAGGKLLVVYLCGSFDIIYGRLLQRKG---HFMPPELLQSQFSIL 154

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BLAST of FE672165 vs. SwissProt
Match: GNTK_DICDI (Probable gluconokinase OS=Dictyostelium discoideum GN=DDB_G0284557 PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.875e-23
Identity = 70/178 (39.33%), Postives = 97/178 (54.49%), Query Frame = 2

Query:   92 VLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINK----KGLVLGCSALKKQYR-EILRSADPDYKWESYASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFR-----VDATLSPESIVNTI 595
            ++VIMGVSG+GKTTIG  +   +   + DAD+FHS+ NKEKM  GIPL D+DRKPWL S+   + E+L N+       V  CSALK  YR +I  + + D         + FILL    ++L  RL NR     H+   +LL SQL +L +     +       +D + S + IV  I
Sbjct:   29 IIVIMGVSGSGKTTIGNAIASSLGCGFNDADEFHSEENKEKMRSGIPLNDDDRKPWLSSINKRMIEFLNNENDGANDHVFTCSALKSTYRDQISNNINKD--------NLLFILLQGSKQLLSERLQNRK---NHFFNPNLLDSQLSILELPTQSELSNHHYAFIDISNSVDEIVEEI 195

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BLAST of FE672165 vs. SwissProt
Match: GNTK_HUMAN (Probable gluconokinase OS=Homo sapiens GN=C9orf103 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 7.123e-23
Identity = 66/177 (37.29%), Postives = 97/177 (54.80%), Query Frame = 2

Query:   95 LVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASA---------VNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDS-ERIFRVDATLSPESIVNTI 595
            L++MGVSG+GK+T+G  L  ++ +K+ DADD+H + N+ KM  GIPL D+DR PWL +L D +   + + + +VL CSALKK YR+IL         +   S          +  + L    EV+  RL  R  EG H+MP  LLQSQ + L    + E   ++    +   I+ TI
Sbjct:    7 LLVMGVSGSGKSTVGALLASELGWKFYDADDYHPEENRRKMGKGIPLNDQDRIPWLCNLHDILLRDVASGQRVVLACSALKKTYRDILTQGKDGVALKCEESGKEAKQAEMQLLVVHLSGSFEVISGRLLKR--EG-HFMPPELLQSQFETLEPPAAPENFIQISVDKNVSEIIATI 180

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BLAST of FE672165 vs. SwissProt
Match: GNTK_SCHPO (Probable gluconokinase OS=Schizosaccharomyces pombe GN=SPAC4G9.12 PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 4.617e-22
Identity = 62/157 (39.49%), Postives = 92/157 (58.60%), Query Frame = 2

Query:   68 PMTSSNAP---VLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINK--KGLVLGCSALKKQYREILRSADPDYKWESYASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLL 523
            P+  +N P   V V++G +G+GKTT+ + + + + ++Y++ DD H ++N EKMS G PL D DR  WL +   A    L  +  KG+VL CSALK+ YR+ILRS+      E   + + FI L    E LI R  +R     HYM A +++SQL +L
Sbjct:    5 PINPTNQPYKYVFVVIGPAGSGKTTMAKAVSEKLGFEYIEGDDLHPKANIEKMSQGHPLNDNDRWGWLHNCGGACAMELDKESIKGVVLTCSALKRSYRDILRSS-----LEHRPAILRFIYLAASRETLIKRTTSRK---NHYMKADMVESQLAIL 153

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BLAST of FE672165 vs. SwissProt
Match: GNTK_ECOLI (Thermoresistant gluconokinase OS=Escherichia coli (strain K12) GN=gntK PE=1 SV=4)

HSP 1 Score: 95.5153 bits (236), Expect = 2.145e-19
Identity = 53/150 (35.33%), Postives = 83/150 (55.33%), Query Frame = 2

Query:   74 TSSNAPVLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLL 523
            T+ +  + V+MGVSG+GK+ +   +   +   +LD D  H + N EKM+ G PL D+DRKPWL++L DA        K  ++ CSALKK YR++LR  +P+         ++FI L    +V+ +RL  R     H+    +L +Q + L
Sbjct:    4 TNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHPRRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGNPN---------LSFIYLKGDFDVIESRLKARKG---HFFKTQMLVTQFETL 141

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BLAST of FE672165 vs. SwissProt
Match: GNTK_YEAST (Probable gluconokinase OS=Saccharomyces cerevisiae GN=YDR248C PE=1 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.699e-16
Identity = 54/161 (33.54%), Postives = 86/161 (53.42%), Query Frame = 2

Query:   74 TSSNAPVLVIMGVSGAGKTTIGQRLEKDIK-----YKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLR-DAIKEYLINKKGL-VLGCSALKKQYREILRSADPDYKWESYASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDD 535
            T     V+V+ G +G GK+TI   L  + K      K+++ DD H  +N EKM+ GIPL D+DR  WL+ +  ++ K     K+ L ++ CS+LKK+YR+++R   P+       S  +FI L      ++ RL  R     H+M A +++SQ   L + D
Sbjct:    7 TMGKFKVIVLAGTAGTGKSTIAGELIHEFKDIYPDLKFIEGDDLHPPANVEKMTRGIPLNDDDRWDWLKKVAVESTKAAASTKEHLSIVACSSLKKKYRDLIRHTCPE-------SEFHFIFLYASKIEVLKRLKTRKG---HFMKADMMESQFRDLELPD 157

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BLAST of FE672165 vs. TrEMBL
Match: B9S1Y6_RICCO (Thermosensitive gluconokinase, putative OS=Ricinus communis GN=RCOM_1324370 PE=4 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 1.013e-58
Identity = 115/176 (65.34%), Postives = 145/176 (82.39%), Query Frame = 2

Query:   71 MTSS-NAPVLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            MTS      +VIMGVSGAGK+TIG+ L KD+K K+LDADDFHSQSN+EKM  GIPL++EDR PWL+SL++A++E LI  K ++LGCS+L+ +YREILRSADP+Y+  SY S V F+LLDV AEVL  RLN RAAEG+H+MPA+LLQSQLDLL++DDSE I++VDA  SP +IV+ I
Sbjct:    1 MTSDFQGKAIVIMGVSGAGKSTIGEMLAKDLKCKFLDADDFHSQSNREKMCQGIPLSEEDRIPWLQSLQEAVRESLIVGKIVILGCSSLQNRYREILRSADPNYQLGSYVSVVKFVLLDVNAEVLAARLNKRAAEGKHFMPATLLQSQLDLLHVDDSEGIYKVDAAQSPRAIVDAI 176

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BLAST of FE672165 vs. TrEMBL
Match: B9S1Y5_RICCO (Thermosensitive gluconokinase, putative OS=Ricinus communis GN=RCOM_1324360 PE=4 SV=1)

HSP 1 Score: 224.942 bits (572), Expect = 3.258e-57
Identity = 111/167 (66.47%), Postives = 140/167 (83.83%), Query Frame = 2

Query:   95 LVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            +VIMGVSGAGK+TIGQ L +++K  +LDAD FHSQSNKEKM  GIPL++EDR PWL+SL++A++E L   + ++LGCS+L+ QYREILRSADP Y+  SY SAV F+LLDV AEVL+ RLN RAAEG+H+MPA+LLQSQL+ L+IDDSE I++VDAT SP +IVN I
Sbjct:   10 IVIMGVSGAGKSTIGQMLAEELKCDFLDADVFHSQSNKEKMRQGIPLSEEDRIPWLQSLQEALRESLTAGETIILGCSSLQNQYREILRSADPYYQLGSYVSAVKFVLLDVSAEVLVERLNKRAAEGKHFMPATLLQSQLEFLHIDDSEGIYKVDATQSPRAIVNVI 176

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BLAST of FE672165 vs. TrEMBL
Match: B9IPI0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_738338 PE=4 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 4.705e-56
Identity = 111/167 (66.47%), Postives = 136/167 (81.44%), Query Frame = 2

Query:   95 LVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            +VIMGVSGAGK+TIG+ L K +   +LDADDFH QSNK+KM  GIPLT+EDR PWLE L+DA++E LI+ K +VL CSAL+KQYREILRSAD DY   S+ SAV F+LLD  AEVL  RL+ RAAEG+H+MPA LLQSQL+LL IDDSE + +VDATL+P+++VN I
Sbjct:   10 IVIMGVSGAGKSTIGELLAKALDCSFLDADDFHPQSNKDKMHQGIPLTEEDRIPWLEILQDALRESLISGKTVVLSCSALQKQYREILRSADSDYHHGSFNSAVKFVLLDAKAEVLAERLDKRAAEGKHFMPAKLLQSQLELLQIDDSEALCKVDATLNPQALVNAI 176

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BLAST of FE672165 vs. TrEMBL
Match: D7T7U3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_39.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00024450001 PE=4 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 1.326e-50
Identity = 100/167 (59.88%), Postives = 128/167 (76.65%), Query Frame = 2

Query:   95 LVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            +VIMGV G+GK+TIG  L K +   +LDADDFH +SNK+KM  GI L+D+DR PWLE+LR+A++E+L++   +VLGCSAL+K YREILRSADP+Y+  S+ S V F+LLD  AEV+  RL  RAAEG H+MP +LLQSQLD L +D SE I +VDAT SP+  VN I
Sbjct:   13 VVIMGVCGSGKSTIGNMLAKVLNCSFLDADDFHPESNKDKMKRGIALSDKDRIPWLETLRNALREHLLSNNTVVLGCSALQKNYREILRSADPNYEHGSFESMVKFVLLDARAEVIAARLEKRAAEGNHFMPPNLLQSQLDSLQLDPSEGILKVDATQSPDDTVNGI 179

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BLAST of FE672165 vs. TrEMBL
Match: A5BY26_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_019218 PE=4 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 5.038e-50
Identity = 99/167 (59.28%), Postives = 127/167 (76.05%), Query Frame = 2

Query:   95 LVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            +VIMGV G+GK+TIG  L K +   +LDADDFH +SNK+KM  GI L+D+DR PWLE+LR+A++E+L++   +VLGCSAL+K YREILRSADP+Y+  S+ S V F+LLD  AEV+  RL  RAAEG H+MP + LQSQLD L +D SE I +VDAT SP+  VN I
Sbjct:    3 VVIMGVCGSGKSTIGNMLAKVLNCSFLDADDFHPESNKDKMKRGIALSDKDRIPWLETLRNALREHLLSNNTVVLGCSALQKNYREILRSADPNYEHGSFESMVKFVLLDARAEVIAARLEKRAAEGNHFMPPNXLQSQLDSLQLDPSEGILKVDATQSPDDTVNGI 169

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BLAST of FE672165 vs. TrEMBL
Match: D7L873_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_674426 PE=4 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 1.466e-49
Identity = 98/169 (57.99%), Postives = 131/169 (77.51%), Query Frame = 2

Query:   92 VLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASA-VNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            V+VIMGVSGAGK+TIG+ L K +   +LDADDFHS SN++KM  GI L+DEDR PWLE +++++++ L++ + +VL CS+L+KQYREILR +DPDY+  SY S  V F+LL+  AEV+  RL  RA+EGEH+MP +LLQSQ DLL  D+ E+IF++   LSPE IVNTI
Sbjct:   11 VIVIMGVSGAGKSTIGKLLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKIQESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYEQGSYTSCKVKFVLLEGNAEVIAARLQKRASEGEHFMPLTLLQSQFDLLQADECEKIFKISVILSPEVIVNTI 179

[Close]

BLAST of FE672165 vs. TrEMBL
Match: Q8LDN6_ARATH (Putative gluconokinase OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 4.265e-49
Identity = 98/169 (57.99%), Postives = 130/169 (76.92%), Query Frame = 2

Query:   92 VLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASA-VNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            V+VIMGVSGAGK+TIG+ L K +   +LDADDFHS SN++KM  GI L+DEDR PWLE +++++++ L++ + +VL CS+L+KQYREILR +DPDYK  SY S  V F+LL+  AEV+  RL  RA+E EH+MP +LLQSQ DLL  D+ E+IF++   LSPE IVNTI
Sbjct:   11 VIVIMGVSGAGKSTIGKMLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKIQESLRKRLLDVETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAARLQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTI 179

[Close]

BLAST of FE672165 vs. TrEMBL
Match: B3H6H9_ARATH (Uncharacterized protein At2g16790.2 OS=Arabidopsis thaliana GN=At2g16790 PE=4 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 9.502e-49
Identity = 97/173 (56.07%), Postives = 131/173 (75.72%), Query Frame = 2

Query:   80 SNAPVLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASA-VNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            ++  V+ IMGVSGAGK+TIG+ L K +   +LDADDFHS SN++KM  GI L+DEDR PWLE +++++++ L++ + +VL CS+L+KQYREILR +DPDYK  SY S  V F+LL+  AEV+  RL  RA+E EH+MP +LLQSQ DLL  D+ E+IF++   LSPE IVNTI
Sbjct:    5 NDGKVIAIMGVSGAGKSTIGKMLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKIQESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAARLQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTI 177

[Close]

BLAST of FE672165 vs. TrEMBL
Match: Q9SLE0_ARATH (Putative gluconokinase OS=Arabidopsis thaliana GN=At2g16790 PE=2 SV=2)

HSP 1 Score: 196.438 bits (498), Expect = 1.241e-48
Identity = 97/169 (57.40%), Postives = 129/169 (76.33%), Query Frame = 2

Query:   92 VLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASA-VNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            V+ IMGVSGAGK+TIG+ L K +   +LDADDFHS SN++KM  GI L+DEDR PWLE +++++++ L++ + +VL CS+L+KQYREILR +DPDYK  SY S  V F+LL+  AEV+  RL  RA+E EH+MP +LLQSQ DLL  D+ E+IF++   LSPE IVNTI
Sbjct:   11 VIAIMGVSGAGKSTIGKMLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKIQESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAARLQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTI 179

[Close]

BLAST of FE672165 vs. TrEMBL
Match: D5AAY1_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 1.329e-42
Identity = 94/194 (48.45%), Postives = 124/194 (63.92%), Query Frame = 2

Query:   95 LVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWES---------------------------YASAVNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            +VIMGV+G+GK+TIG  L K I   ++DADDFH   NKEKM  GIPLTDEDR PWLE++RD + +Y+I  K  V+ CSAL+ +YREILR+ADP+Y+  S                           ++  V F+ L  PAE++ +RL +RA EG H+M   LLQSQ+DLL IDD+E +F +DATL P+ IV  I
Sbjct:    6 IVIMGVTGSGKSTIGSCLAKAIHCMFIDADDFHCFENKEKMRSGIPLTDEDRLPWLEAVRDTMIDYIIEGKIAVVACSALQPKYREILRTADPEYEPASSDGFSCEKIYGNSFSTSIEVENCAKAPFSRRVVFLWLQAPAELIASRLESRAKEGAHFMSPGLLQSQMDLLQIDDAEGVFCIDATLDPDLIVENI 199

[Close]

BLAST of FE672165 vs. TAIR peptide
Match: AT2G16790.2 (| Symbols: | P-loop containing nucleoside triphosphate hydrolases superfamily protein | chr2:7284093-7284901 REVERSE LENGTH=187)

HSP 1 Score: 196.823 bits (499), Expect = 7.164e-51
Identity = 97/173 (56.07%), Postives = 131/173 (75.72%), Query Frame = 2

Query:   80 SNAPVLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASA-VNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            ++  V+ IMGVSGAGK+TIG+ L K +   +LDADDFHS SN++KM  GI L+DEDR PWLE +++++++ L++ + +VL CS+L+KQYREILR +DPDYK  SY S  V F+LL+  AEV+  RL  RA+E EH+MP +LLQSQ DLL  D+ E+IF++   LSPE IVNTI
Sbjct:    5 NDGKVIAIMGVSGAGKSTIGKMLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKIQESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAARLQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTI 177

[Close]

BLAST of FE672165 vs. TAIR peptide
Match: AT2G16790.1 (| Symbols: | P-loop containing nucleoside triphosphate hydrolases superfamily protein | chr2:7284093-7284901 REVERSE LENGTH=189)

HSP 1 Score: 196.438 bits (498), Expect = 9.357e-51
Identity = 97/169 (57.40%), Postives = 129/169 (76.33%), Query Frame = 2

Query:   92 VLVIMGVSGAGKTTIGQRLEKDIKYKYLDADDFHSQSNKEKMSMGIPLTDEDRKPWLESLRDAIKEYLINKKGLVLGCSALKKQYREILRSADPDYKWESYASA-VNFILLDVPAEVLITRLNNRAAEGEHYMPASLLQSQLDLLNIDDSERIFRVDATLSPESIVNTI 595
            V+ IMGVSGAGK+TIG+ L K +   +LDADDFHS SN++KM  GI L+DEDR PWLE +++++++ L++ + +VL CS+L+KQYREILR +DPDYK  SY S  V F+LL+  AEV+  RL  RA+E EH+MP +LLQSQ DLL  D+ E+IF++   LSPE IVNTI
Sbjct:   11 VIAIMGVSGAGKSTIGKMLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKIQESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAARLQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTI 179

The following BLAST results are available for this feature:

BLAST of FE672165 vs. SwissProt
BLAST of FE672165 vs. TrEMBL
BLAST of FE672165 vs. TAIR peptide

BLAST of FE672165 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
IDNK_ECOLI	3.650e-27	43.95	Thermosensitive gluconokinase OS=Escherichia coli ...	[more]
GNTK_XENTR	2.003e-25	39.43	Probable gluconokinase OS=Xenopus tropicalis PE=2 ...	[more]
GNTK_RAT	1.298e-24	41.83	Probable gluconokinase OS=Rattus norvegicus PE=2 S...	[more]
GNTK_MOUSE	1.875e-23	40.79	Probable gluconokinase OS=Mus musculus PE=2 SV=2	[more]
GNTK_DICDI	1.875e-23	39.33	Probable gluconokinase OS=Dictyostelium discoideum...	[more]
GNTK_HUMAN	7.123e-23	37.29	Probable gluconokinase OS=Homo sapiens GN=C9orf103...	[more]
GNTK_SCHPO	4.617e-22	39.49	Probable gluconokinase OS=Schizosaccharomyces pomb...	[more]
GNTK_ECOLI	2.145e-19	35.33	Thermoresistant gluconokinase OS=Escherichia coli ...	[more]
GNTK_YEAST	1.699e-16	33.54	Probable gluconokinase OS=Saccharomyces cerevisiae...	[more]

BLAST of FE672165 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9S1Y6_RICCO	1.013e-58	65.34	Thermosensitive gluconokinase, putative OS=Ricinus...	[more]
B9S1Y5_RICCO	3.258e-57	66.47	Thermosensitive gluconokinase, putative OS=Ricinus...	[more]
B9IPI0_POPTR	4.705e-56	66.47	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7T7U3_VITVI	1.326e-50	59.88	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BY26_VITVI	5.038e-50	59.28	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7L873_ARALY	1.466e-49	57.99	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
Q8LDN6_ARATH	4.265e-49	57.99	Putative gluconokinase OS=Arabidopsis thaliana PE=...	[more]
B3H6H9_ARATH	9.502e-49	56.07	Uncharacterized protein At2g16790.2 OS=Arabidopsis...	[more]
Q9SLE0_ARATH	1.241e-48	57.40	Putative gluconokinase OS=Arabidopsis thaliana GN=...	[more]
D5AAY1_PICSI	1.329e-42	48.45	Putative uncharacterized protein OS=Picea sitchens...	[more]

BLAST of FE672165 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G16790.2	7.164e-51	56.07	\| Symbols: \| P-loop containing nucleoside triphos...	[more]
AT2G16790.1	9.357e-51	57.40	\| Symbols: \| P-loop containing nucleoside triphos...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000623	Shikimate kinase	PRINTS	PR01100	SHIKIMTKNASE	coord: 20..35 score: 8.7e-07coord: 44..57 score: 8.7e-07coord: 123..140 score: 8.7
IPR000623	Shikimate kinase	PFAM	PF01202	SKI	coord: 26..144 score: 9.8
IPR006001	Carbohydrate kinase, thermoresistant glucokinase	TIGRFAMs	TIGR01313	therm_gnt_kin: carbohydrate kinase, the	coord: 20..185 score: 1.5
None	No IPR available	GENE3D	3.40.50.300	no description	coord: 15..186 score: 3.4
None	No IPR available	SEG	seg	seg	coord: 2..9 score
None	No IPR available	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 12..186 score: 8.4
None	No IPR available	SEG	seg	seg	coord: 34..48 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE672165 ID=FE672165; Name=FE672165; organism=Cicer arietinum; type=EST; length=596bp
GGCTGCAGGAATTCGAATTCCAGCACTTGAGTTCTAAATTCTCTCTCTCT
CTCTCTTATTGTTACATCCCATGACTTCAAGCAACGCTCCTGTTCTCGTC
ATCATGGGTGTCAGCGGGGCCGGAAAAACCACAATAGGTCAAAGGCTGGA
AAAAGATATTAAATATAAGTATCTTGATGCTGATGATTTTCATTCTCAAT
CAAACAAAGAAAAGATGAGTATGGGAATCCCACTTACAGATGAAGACAGA
AAGCCATGGCTTGAATCACTGAGAGATGCCATAAAAGAATACTTAATTAA
CAAAAAGGGTTTGGTTCTTGGTTGCTCTGCTTTGAAGAAGCAATATAGAG
AAATACTTAGATCAGCTGATCCTGATTATAAATGGGAGAGTTATGCAAGT
GCTGTTAACTTTATTCTATTGGATGTTCCTGCTGAAGTGTTAATTACTCG
ATTAAATAACAGGGCTGCAGAAGGAGAACATTATATGCCTGCATCTCTTC
TGCAATCTCAGTTAGATTTGCTTAACATTGATGATTCTGAACGAATATTT
AGAGTTGATGCTACTTTAAGTCCTGAATCCATTGTCAATACCATTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004765	shikimate kinase activity
GO:0005524	ATP binding
GO:0016301	kinase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000623	Shikimate_kinase/TSH1
IPR006001	Therm_gnt_kin

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