FE673258

EST Overview


Name	FE673258
Unique Name	FE673258
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	610

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FE673258 vs. SwissProt
Match: PAPS1_CAVPO (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 OS=Cavia porcellus GN=PAPSS1 PE=2 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 9.668e-31
Identity = 69/133 (51.88%), Postives = 90/133 (67.67%), Query Frame = 2

Query:  212 ELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            EL V ++K  L K +A SLP ++++++D++WV VL+EGWATPLGGFMRERE+LQ LHF+ L   DG V+N+SVPIVL   +  K R+      AL    G  VAIL + E ++H KEER AR WGTT    PY
Sbjct:  236 ELYVPENKLHLAKTDAESLPALQINKVDMQWVQVLAEGWATPLGGFMREREYLQCLHFDCLL--DGGVINLSVPIVLTATEEDKERLDGCTAFALIYE-GRRVAILRNPEFFEHRKEERCARQWGTTCKSHPY 365

[Close]

BLAST of FE673258 vs. SwissProt
Match: PAPS2_HUMAN (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens GN=PAPSS2 PE=1 SV=2)

HSP 1 Score: 130.954 bits (328), Expect = 4.798e-30
Identity = 68/133 (51.13%), Postives = 91/133 (68.42%), Query Frame = 2

Query:  212 ELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            EL V ++K D  + EA +LP + ++++D++WV VLSEGWATPL GFMRE+E+LQ +HF++L LDDG V+NMS+PIVL +    K R+    K  L    G  VAIL D E Y+H KEER +R WGTT    P+
Sbjct:  226 ELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQVMHFDTL-LDDG-VINMSIPIVLPVSAEDKTRLEGCSKFVLAHG-GRRVAILRDAEFYEHRKEERCSRVWGTTCTKHPH 355

[Close]

BLAST of FE673258 vs. SwissProt
Match: PAPS1_HUMAN (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 OS=Homo sapiens GN=PAPSS1 PE=1 SV=2)

HSP 1 Score: 130.183 bits (326), Expect = 8.185e-30
Identity = 68/133 (51.13%), Postives = 88/133 (66.17%), Query Frame = 2

Query:  212 ELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            EL V ++K  L K +A +LP +K++++D++WV VL+EGWATPL GFMRERE+LQ LHF+ L   DG V+N+SVPIVL      K R+      AL    G  VAIL + E ++H KEER AR WGTT    PY
Sbjct:  236 ELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLL--DGGVINLSVPIVLTATHEDKERLDGCTAFALMYE-GRRVAILRNPEFFEHRKEERCARQWGTTCKNHPY 365

[Close]

BLAST of FE673258 vs. SwissProt
Match: PAPS1_MOUSE (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 OS=Mus musculus GN=Papss1 PE=2 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.069e-29
Identity = 68/133 (51.13%), Postives = 88/133 (66.17%), Query Frame = 2

Query:  212 ELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            EL V ++K  L K +A +LP +K++++D++WV VL+EGWATPL GFMRERE+LQ LHF+ L   DG V+N+SVPIVL      K R+      AL    G  VAIL + E ++H KEER AR WGTT    PY
Sbjct:  236 ELYVPENKLHLAKTDAEALPALKINKVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLL--DGGVINLSVPIVLTATHEDKERLDGCTAFALVYE-GRRVAILRNPEFFEHRKEERCARQWGTTCKNHPY 365

[Close]

BLAST of FE673258 vs. SwissProt
Match: PAPS2_MOUSE (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Mus musculus GN=Papss2 PE=1 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 5.866e-28
Identity = 68/138 (49.28%), Postives = 92/138 (66.67%), Query Frame = 2

Query:  212 ELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLD-----DGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            EL V ++K D  + EA +LP + ++++D++WV +LSEGWATPL GFMRE+E+LQTLHF++L LD     DG V+NMS+PIVL +    K R+    K AL    G  VA+L D E Y+H KEER +R WGT     P+
Sbjct:  227 ELFVPENKVDQIRAEAETLPSLPITKLDLQWVQILSEGWATPLKGFMREKEYLQTLHFDTL-LDGVVPRDG-VINMSIPIVLPVSADDKARLEGCSKFALMYE-GRRVALLQDPEFYEHRKEERCSRVWGTATAKHPH 361

[Close]

BLAST of FE673258 vs. SwissProt
Match: PAPSS_URECA (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase OS=Urechis caupo PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.802e-27
Identity = 67/146 (45.89%), Postives = 89/146 (60.96%), Query Frame = 2

Query:  176 NGLIEPDGGKLV-ELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            NG++      +V EL V +S  +  K E   LP ++++++D +WV VLSEGWATPL GFMRERE+LQ+ HF  L   DG V N S+PIVL +    K R+  S   AL    G  +AIL   E Y+H KEER +R +GT+  G PY
Sbjct:  208 NGVVPESAVNIVKELFVPESGLEHAKAEIVDLPTMEITKLDTQWVQVLSEGWATPLTGFMREREYLQSQHFGCLL--DGGVTNQSIPIVLPVHTADKDRLEGSSAFAL-SYEGKRIAILRTPEFYEHRKEERCSRQFGTSNAGQPY 350

[Close]

BLAST of FE673258 vs. SwissProt
Match: SAT_HYPBU (Sulfate adenylyltransferase OS=Hyperthermus butylicus (strain DSM 5456 / JCM 9403) GN=sat PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.476e-10
Identity = 43/139 (30.94%), Postives = 76/139 (54.68%), Query Frame = 2

Query:  191 PDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLP 607
            P GG+LV+  V D +R+  + EA  LP I+L+      V  ++ G  +PL GFM + ++L  L  + +RL +   +  ++PI+L +D  +   + +   +AL  + G P+A++   EIY   ++E  A+ + TT P  P
Sbjct:    5 PHGGRLVDRTVSDKRRERLREEARELPAIRLTAGLAADVANIAHGVYSPLEGFMLQEDYLSVL--DEMRLSND--LPWTIPIILDVDPGEIAGVREGDDIALVYN-GKPIALMRVEEIYGWDRKEYAAKVFKTTDPAHP 138

[Close]

BLAST of FE673258 vs. SwissProt
Match: SATC1_SORC5 (Probable bifunctional SAT/APS kinase 1 OS=Sorangium cellulosum (strain So ce56) GN=sat1/cysC1 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 9.408e-10
Identity = 43/143 (30.07%), Postives = 65/143 (45.45%), Query Frame = 2

Query:  179 GLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLP 607
            GL  P GG+LV+  V    R         LPR++L       + ++  G  +PL GFM  R++L+ +     RL+ G V   S+PI LA+      R+    +VAL    G  V +L  ++ +   KE      +G T  G P
Sbjct:  189 GLAPPHGGELVDRFVRGDARQRLLERVAGLPRVRLDERGASDLELIGNGAYSPLKGFMTSRDYLRVV--RERRLESGLV--WSIPITLAVPGEDAARLSLGSEVALASPDGRVVGVLELVDRWTPDKEVEARNVYGATDEGHP 327

[Close]

BLAST of FE673258 vs. SwissProt
Match: SAT_SYMTH (Sulfate adenylyltransferase OS=Symbiobacterium thermophilum GN=sat PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.737e-9
Identity = 38/141 (26.95%), Postives = 72/141 (51.06%), Query Frame = 2

Query:  185 IEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLP 607
            I P GG+LV+  +    R+     A  LPR++L   +   + ++ +G  +PL GFM + ++   +    +RL  G +   ++P+ LA+   +   I + +++AL D  G  +A++   E + + +    AR +GTT P  P
Sbjct:    5 IAPHGGRLVDRWLRGPAREEALERARRLPRVRLDAREAADLEMIGDGALSPLTGFMGQADYRSVV--AEMRLASGLL--WALPVTLAVSRAEAESIREGEEIALEDPGGRLMAVMRVAERFAYDRGAEAARCYGTTDPAHP 141

[Close]

BLAST of FE673258 vs. SwissProt
Match: SAT_HALOH (Sulfate adenylyltransferase OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) GN=sat PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 4.669e-9
Identity = 44/138 (31.88%), Postives = 73/138 (52.90%), Query Frame = 2

Query:  182 LIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVAL-FDSLGNPVAILNDIEIYKHPKEERIARTWGTT 592
            +I+P GGKLV  +VE +KR+    +A  +PRI L+R ++  V  ++ G  +PL GF+   ++ + +    +RL DG+V   S+P VL +   +   + + + V L F+      AIL+  E Y    E      +GTT
Sbjct:    1 MIKPHGGKLVNRIVEGAKREELIKKAGEMPRIMLNRDELTAVDNIATGLFSPLEGFLTSEDYNRVV--EEMRLADGTV--WSIPEVLGVTREEADNLKEGQDVGLYFEEDDELYAILHLEEKYTCDPEREAELVYGTT 134

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BLAST of FE673258 vs. TrEMBL
Match: B7FHX4_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 335.495 bits (859), Expect = 1.838e-90
Identity = 166/194 (85.57%), Postives = 181/194 (93.30%), Query Frame = 2

Query:   29 MASMATLLSKTPFPSNSLTKKIDTHFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MASMATLLSKT FPS+SL K  DTHFAP +RVN+A+G K+RT++++R+S+GLIEPDGGKLVELVVE+SKRDLKKGEA SLPRIKLS+IDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRL+DGS VNMSVPIVLAIDD QKHRIG+S KVALFDS GNPVAIL DIEIYKH KEERIARTWGTTAPGLPY
Sbjct:    1 MASMATLLSKTSFPSHSLFKTFDTHFAPPIRVNVAIGTKARTNQRLRVSSGLIEPDGGKLVELVVEESKRDLKKGEALSLPRIKLSKIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLNDGSFVNMSVPIVLAIDDDQKHRIGESTKVALFDSKGNPVAILKDIEIYKHLKEERIARTWGTTAPGLPY 194

[Close]

BLAST of FE673258 vs. TrEMBL
Match: Q8SAG1_SOYBN (ATP sulfurylase OS=Glycine max PE=2 SV=2)

HSP 1 Score: 312.768 bits (800), Expect = 1.275e-83
Identity = 153/191 (80.10%), Postives = 170/191 (89.01%), Query Frame = 2

Query:   38 MATLLSKTPFPSNSLTKKIDTHFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MAT  ++T FPS+SL+K  DTHFAP+ +VN+ +  ++R H  +R+SN LIEPDGGKLVELVV D +RDLKKGEA SLPRIKLSRID+EWVHVLSEGWATPL GFMRE EFLQTLHFNSLRLDDGSVVNMSVPIVLAIDD QKHRIGD+KKVALFDS G+PVAILN+IEIYKHPKEERIARTWGT APGLPY
Sbjct:    1 MATFFAQTSFPSHSLSKTFDTHFAPAPKVNVFVNFRARRHVGVRVSNALIEPDGGKLVELVVTDFERDLKKGEALSLPRIKLSRIDLEWVHVLSEGWATPLKGFMREAEFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDAQKHRIGDNKKVALFDSKGDPVAILNNIEIYKHPKEERIARTWGTIAPGLPY 191

[Close]

BLAST of FE673258 vs. TrEMBL
Match: A5AV70_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_002534 PE=4 SV=1)

HSP 1 Score: 283.108 bits (723), Expect = 1.082e-74
Identity = 142/196 (72.45%), Postives = 165/196 (84.18%), Query Frame = 2

Query:   29 MASMATLLSKTPFPSNSLT--KKIDTHFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MAS++TL +KTP PS SL   +   +HF P+ R+ + L +K+RTH+K+R+S+ LIEPDGGKLVEL VE+S RD+KK EA  +PRIKLSRID+EWVHVLSEGWA+PL GFMRE EFLQTLHFNSLRLDDGS VNMSVPIVLAIDD +KH+IGDS KVAL DS  N +AIL+ IEIYKH KEERIARTWGTTAPGLPY
Sbjct:    1 MASISTLFTKTPNPSPSLCLPRTPKSHFTPAFRLPIPLHSKTRTHQKLRVSSALIEPDGGKLVELFVEESLRDVKKREALRMPRIKLSRIDLEWVHVLSEGWASPLRGFMRESEFLQTLHFNSLRLDDGSFVNMSVPIVLAIDDAEKHQIGDSTKVALVDSKDNTIAILSSIEIYKHHKEERIARTWGTTAPGLPY 196

[Close]

BLAST of FE673258 vs. TrEMBL
Match: Q1HL01_CAMSI (ATP sulfurylase OS=Camellia sinensis GN=APS2 PE=2 SV=2)

HSP 1 Score: 270.781 bits (691), Expect = 5.558e-71
Identity = 138/194 (71.13%), Postives = 161/194 (82.99%), Query Frame = 2

Query:   29 MASMATLLSKTPFPSNSLTKKIDTHFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MASMA L +K+P PS S  K   TH++  +++ L+  +  +TH+KIRIS GLI+PDGGKLVEL V +S+RD KK +A SLPRIKLSRID++WVHVLSEGWA+PL GFMRE EFLQTLHFNSLRL DGSVVNMSVPIVLA+DD QK RIG+S  VAL DS  +PVAIL++IEIYKH KEERIARTWGTTAPGLPY
Sbjct:    1 MASMALLFNKSPNPSLSFPKTHKTHYSTHLKLPLSHHSSPKTHRKIRISCGLIDPDGGKLVELFVSESERDEKKRKAVSLPRIKLSRIDLQWVHVLSEGWASPLKGFMRESEFLQTLHFNSLRLGDGSVVNMSVPIVLAVDDSQKGRIGESTSVALVDSGDDPVAILSNIEIYKHNKEERIARTWGTTAPGLPY 194

[Close]

BLAST of FE673258 vs. TrEMBL
Match: D7L2G4_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_479799 PE=4 SV=1)

HSP 1 Score: 270.396 bits (690), Expect = 7.259e-71
Identity = 141/194 (72.68%), Postives = 157/194 (80.93%), Query Frame = 2

Query:   29 MASMATLLSKTPFPSNSLTKKIDTHFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MASMA +LSKTPF S  LTK   T   P   V+    +  R +  IR   GLI PDGGKLVEL+VE+ KR  KK EA  LPR++L+ ID++W+HVLSEGWA+PLGGFMRE EFLQTLHFNSLRLDDGSVVNMSVPIVLAIDD QK RIG+SK+VALFDS GNPVAIL+DIEIYKHPKEERIARTWGTTAPGLPY
Sbjct:    1 MASMAAILSKTPFLSQPLTKPSPTSDIPFAAVSFPSKSLRRRNGSIRA--GLIAPDGGKLVELIVEEPKRREKKHEAAELPRVELTTIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESKRVALFDSDGNPVAILSDIEIYKHPKEERIARTWGTTAPGLPY 192

[Close]

BLAST of FE673258 vs. TrEMBL
Match: B9HUQ5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_658460 PE=4 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 1.238e-70
Identity = 131/194 (67.53%), Postives = 163/194 (84.02%), Query Frame = 2

Query:   29 MASMATLLSKTPFPSNSLTKKIDTHFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MA+M+TL +KT + S+SL K ++THF P+++++ A        K++RI  GLI+PDGGKLV+L  E S++DLKK EA SLP++KL++ID++WVHVLSEGWA+PL GFMRE EFLQTLHFNSLRL++GSVVNMSVPIVLAIDD+QK  IG+SK+VAL DS  N +AI NDIEIYKHPKEERIARTWGT+APGLPY
Sbjct:    1 MATMSTLFTKTSYSSHSLPKPLNTHFGPTLKLSFA---PKTQWKRVRIQAGLIDPDGGKLVQLFAEKSQQDLKKKEAISLPKVKLTKIDIQWVHVLSEGWASPLRGFMRESEFLQTLHFNSLRLENGSVVNMSVPIVLAIDDLQKQGIGESKRVALVDSDDNTIAIFNDIEIYKHPKEERIARTWGTSAPGLPY 191

[Close]

BLAST of FE673258 vs. TrEMBL
Match: B9HKB7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_720943 PE=4 SV=1)

HSP 1 Score: 266.544 bits (680), Expect = 1.048e-69
Identity = 130/194 (67.01%), Postives = 163/194 (84.02%), Query Frame = 2

Query:   29 MASMATLLSKTPFPSNSLTKKIDTHFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MA+M+ L +KT +P +SL K  +T F+P+++++ A   +    K++RI +GLI+PDGGKLVEL VE S++D KK EA SLP++KL+ ID++W+HVLSEGWA+PL GFMRE EFLQTLHFNSLRL++GSVVNMSVPIVLAIDD+QK  IG+SK+VAL DS  N VAIL+D+EIYKHPKEERIARTWGTTAPGLPY
Sbjct:    1 MATMSALFTKTSYPPHSLPKSFNTRFSPTLKLSFAPKIQ---WKRVRIQSGLIDPDGGKLVELFVEKSQKDAKKKEAISLPKVKLTMIDIQWLHVLSEGWASPLRGFMRESEFLQTLHFNSLRLENGSVVNMSVPIVLAIDDLQKQSIGESKRVALVDSDDNTVAILSDVEIYKHPKEERIARTWGTTAPGLPY 191

[Close]

BLAST of FE673258 vs. TrEMBL
Match: Q1W2K0_CAMSI (ATP-sulfurylase OS=Camellia sinensis PE=2 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 5.202e-69
Identity = 140/194 (72.16%), Postives = 158/194 (81.44%), Query Frame = 2

Query:   29 MASMATLLSKTPFPSNSLTKKIDTHFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MASMA    KTP+ S S  K   THF+  +++ L+L +  +T  KIRIS GLI PDGGKLVELVVE+S+RD KK +A S+PRIKLSRID++WVHVLSEGWA+PL GFMRE EFLQTLHFN LRLDDGSVVNMSVPIVLAI D QK RIG+S  VAL DS  NPVAIL++IEIYKH KEERIARTWGTTAPGLPY
Sbjct:    1 MASMAAFFIKTPYQSLSFPK---THFSTPLKLPLSLHSNPKT-TKIRISCGLINPDGGKLVELVVEESQRDEKKRQALSMPRIKLSRIDLQWVHVLSEGWASPLRGFMRESEFLQTLHFNLLRLDDGSVVNMSVPIVLAITDSQKSRIGESSAVALVDSGDNPVAILSNIEIYKHNKEERIARTWGTTAPGLPY 190

[Close]

BLAST of FE673258 vs. TrEMBL
Match: Q9SE02_ARATH (ATP sulfurylase OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 263.462 bits (672), Expect = 8.874e-69
Identity = 138/194 (71.13%), Postives = 155/194 (79.90%), Query Frame = 2

Query:   29 MASMATLLSKTPFPSNSLTKKIDTHFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MASMA +LSKTPF S  LTK       P   V+    +KS   +   I  GLI PDGGKLVEL+VE+ KR  KK EA  LPR++L+ ID++W+HVLSEGWA+PLGGFMRE EFLQTLHFNSLRLDDGSVVNMSVPIVLAIDD QK RIG+S +VALF+S GNPVAIL+DIEIYKHPKEERIARTWGTTAPGLPY
Sbjct:    1 MASMAAVLSKTPFLSQPLTKSSPNSDLPFAAVSFP--SKSLRRRVGSIRAGLIAPDGGKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPKEERIARTWGTTAPGLPY 192

[Close]

BLAST of FE673258 vs. TrEMBL
Match: Q9LIK9_ARATH (AT3g22890/F5N5_6 OS=Arabidopsis thaliana GN=At3g22890/F5N5.6 PE=2 SV=1)

HSP 1 Score: 263.462 bits (672), Expect = 8.874e-69
Identity = 138/194 (71.13%), Postives = 155/194 (79.90%), Query Frame = 2

Query:   29 MASMATLLSKTPFPSNSLTKKIDTHFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MASMA +LSKTPF S  LTK       P   V+    +KS   +   I  GLI PDGGKLVEL+VE+ KR  KK EA  LPR++L+ ID++W+HVLSEGWA+PLGGFMRE EFLQTLHFNSLRLDDGSVVNMSVPIVLAIDD QK RIG+S +VALF+S GNPVAIL+DIEIYKHPKEERIARTWGTTAPGLPY
Sbjct:    1 MASMAAVLSKTPFLSQPLTKSSPNSDLPFAAVSFP--SKSLRRRVGSIRAGLIAPDGGKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPKEERIARTWGTTAPGLPY 192

[Close]

BLAST of FE673258 vs. TAIR peptide
Match: AT3G22890.1 (| Symbols: APS1 | ATP sulfurylase 1 | chr3:8112837-8114734 FORWARD LENGTH=463)

HSP 1 Score: 263.462 bits (672), Expect = 6.535e-71
Identity = 138/194 (71.13%), Postives = 155/194 (79.90%), Query Frame = 2

Query:   29 MASMATLLSKTPFPSNSLTKKIDTHFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MASMA +LSKTPF S  LTK       P   V+    +KS   +   I  GLI PDGGKLVEL+VE+ KR  KK EA  LPR++L+ ID++W+HVLSEGWA+PLGGFMRE EFLQTLHFNSLRLDDGSVVNMSVPIVLAIDD QK RIG+S +VALF+S GNPVAIL+DIEIYKHPKEERIARTWGTTAPGLPY
Sbjct:    1 MASMAAVLSKTPFLSQPLTKSSPNSDLPFAAVSFP--SKSLRRRVGSIRAGLIAPDGGKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPKEERIARTWGTTAPGLPY 192

[Close]

BLAST of FE673258 vs. TAIR peptide
Match: AT4G14680.1 (| Symbols: APS3 | Pseudouridine synthase/archaeosine transglycosylase-like family protein | chr4:8413443-8415311 REVERSE LENGTH=465)

HSP 1 Score: 245.358 bits (625), Expect = 1.842e-65
Identity = 129/197 (65.48%), Postives = 155/197 (78.68%), Query Frame = 2

Query:   29 MASMATLLSK-TPFPSNSLTKKIDTHFAPSMRVNLALGAKSRTH--KKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            MASM+T+  K T F S  LTK   +H + S+  +++  + S+T   + I +  GLIEPDGGKLV+LVV + +R  KK EA  LPR++L+ ID++W+HVLSEGWA+PL GFMRE EFLQTLHFN L LDDGSVVNMSVPIVLAIDD QK  IG+SK+V+L DS  NP+AILNDIEIYKHPKEERIARTWGTTAPGLPY
Sbjct:    1 MASMSTVFPKPTSFISQPLTK---SHKSDSVTTSISFPSNSKTRSLRTISVRAGLIEPDGGKLVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGWASPLRGFMRESEFLQTLHFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILNDIEIYKHPKEERIARTWGTTAPGLPY 194

[Close]

BLAST of FE673258 vs. TAIR peptide
Match: AT5G43780.1 (| Symbols: APS4 | Pseudouridine synthase/archaeosine transglycosylase-like family protein | chr5:17589631-17591480 REVERSE LENGTH=469)

HSP 1 Score: 241.121 bits (614), Expect = 3.473e-64
Identity = 124/195 (63.59%), Postives = 155/195 (79.49%), Query Frame = 2

Query:   32 ASMATLLSKTPFPSNSLTKKID-THFAPSMRVNLALGAKSRTHKKIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLP-RIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            +S A ++S +PF S+ L      + +AP   +++    +  + + + + +GLIEPDGGKL+ LVVE+S+R + K EA ++P RIKL+R+D+EWVHVLSEGWA+PL GFMR+ EFLQTLHFNS RL+DGSVVNMSVPIVLAIDD QK RIGDS +V L DS+GNP+AILNDIEIYKHPKEERIARTWGTTA GLPY
Sbjct:    3 SSAAAIVSGSPFRSSPLIHNHHASRYAPGS-ISVVSLPRQVSRRGLSVKSGLIEPDGGKLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLKGFMRQSEFLQTLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILNDIEIYKHPKEERIARTWGTTARGLPY 196

[Close]

BLAST of FE673258 vs. TAIR peptide
Match: AT1G19920.1 (| Symbols: APS2, ASA1 | Pseudouridine synthase/archaeosine transglycosylase-like family protein | chr1:6914835-6916657 REVERSE LENGTH=476)

HSP 1 Score: 197.208 bits (500), Expect = 5.752e-51
Identity = 92/150 (61.33%), Postives = 123/150 (82.00%), Query Frame = 2

Query:  161 KIRISNGLIEPDGGKLVELVVEDSKRDLKKGEAFSLPRIKLSRIDVEWVHVLSEGWATPLGGFMREREFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDVQKHRIGDSKKVALFDSLGNPVAILNDIEIYKHPKEERIARTWGTTAPGLPY 610
            K+ + + LI+PDGG+LVEL+V +++  +KK E+ ++P++KL++ID+EWVHV+SEGWA+PL GFMRE E+LQ+LHFNSLRL +G+ VNMS+PIVLAIDD  K +IG S+ VAL    G+ +  L  +EIYKH KEERIARTWGTT+PGLPY
Sbjct:   57 KMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPLKGFMREDEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDIIGSLRSVEIYKHNKEERIARTWGTTSPGLPY 206

The following BLAST results are available for this feature:

BLAST of FE673258 vs. SwissProt
BLAST of FE673258 vs. TrEMBL
BLAST of FE673258 vs. TAIR peptide

BLAST of FE673258 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PAPS1_CAVPO	9.668e-31	51.88	Bifunctional 3'-phosphoadenosine 5'-phosphosulfate...	[more]
PAPS2_HUMAN	4.798e-30	51.13	Bifunctional 3'-phosphoadenosine 5'-phosphosulfate...	[more]
PAPS1_HUMAN	8.185e-30	51.13	Bifunctional 3'-phosphoadenosine 5'-phosphosulfate...	[more]
PAPS1_MOUSE	1.069e-29	51.13	Bifunctional 3'-phosphoadenosine 5'-phosphosulfate...	[more]
PAPS2_MOUSE	5.866e-28	49.28	Bifunctional 3'-phosphoadenosine 5'-phosphosulfate...	[more]
PAPSS_URECA	3.802e-27	45.89	Bifunctional 3'-phosphoadenosine 5'-phosphosulfate...	[more]
SAT_HYPBU	2.476e-10	30.94	Sulfate adenylyltransferase OS=Hyperthermus butyli...	[more]
SATC1_SORC5	9.408e-10	30.07	Probable bifunctional SAT/APS kinase 1 OS=Sorangiu...	[more]
SAT_SYMTH	2.737e-9	26.95	Sulfate adenylyltransferase OS=Symbiobacterium the...	[more]
SAT_HALOH	4.669e-9	31.88	Sulfate adenylyltransferase OS=Halothermothrix ore...	[more]

BLAST of FE673258 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FHX4_MEDTR	1.838e-90	85.57	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q8SAG1_SOYBN	1.275e-83	80.10	ATP sulfurylase OS=Glycine max PE=2 SV=2	[more]
A5AV70_VITVI	1.082e-74	72.45	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q1HL01_CAMSI	5.558e-71	71.13	ATP sulfurylase OS=Camellia sinensis GN=APS2 PE=2 ...	[more]
D7L2G4_ARALY	7.259e-71	72.68	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9HUQ5_POPTR	1.238e-70	67.53	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9HKB7_POPTR	1.048e-69	67.01	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q1W2K0_CAMSI	5.202e-69	72.16	ATP-sulfurylase OS=Camellia sinensis PE=2 SV=1	[more]
Q9SE02_ARATH	8.874e-69	71.13	ATP sulfurylase OS=Arabidopsis thaliana PE=4 SV=1	[more]
Q9LIK9_ARATH	8.874e-69	71.13	AT3g22890/F5N5_6 OS=Arabidopsis thaliana GN=At3g22...	[more]

BLAST of FE673258 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT3G22890.1	6.535e-71	71.13	\| Symbols: APS1 \| ATP sulfurylase 1 \| chr3:8112837...	[more]
AT4G14680.1	1.842e-65	65.48	\| Symbols: APS3 \| Pseudouridine synthase/archaeosi...	[more]
AT5G43780.1	3.473e-64	63.59	\| Symbols: APS4 \| Pseudouridine synthase/archaeosi...	[more]
AT1G19920.1	5.752e-51	61.33	\| Symbols: APS2, ASA1 \| Pseudouridine synthase/arc...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002650	Sulphate adenylyltransferase	PFAM	PF01747	ATP-sulfurylase	coord: 131..199 score: 3.2
IPR015947	Pseudouridine synthase/archaeosine transglycosylase-like	SUPERFAMILY	88697	PUA domain-like	coord: 59..200 score: 5
None	No IPR available	GENE3D	3.10.400.10	no description	coord: 59..200 score: 2.1
None	No IPR available	PANTHER	PTHR11055	ADENYLSULFATE KINASE/SULFATE ADENYLTRANSFERASE	coord: 71..200 score: 2.6
None	No IPR available	PANTHER	PTHR11055:SF2	SULFATE ADENYLYLTRANSFERASE	coord: 71..200 score: 2.6
None	No IPR available	SEG	seg	seg	coord: 27..33 score
None	No IPR available	SEG	seg	seg	coord: 1..12 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE673258 ID=FE673258; Name=FE673258; organism=Cicer arietinum; type=EST; length=610bp
ATTGTTTTAATCATTCAGAGCCATTTCAATGGCTTCCATGGCTACCCTTT
TGTCCAAAACCCCTTTCCCTTCTAACTCTTTGACGAAAAAAATCGATACC
CATTTCGCTCCTTCCATGAGAGTCAACTTAGCTTTGGGTGCAAAATCAAG
GACCCACAAAAAGATTCGAATTTCGAACGGGTTGATTGAACCAGACGGTG
GGAAATTGGTTGAGCTCGTGGTTGAAGATTCCAAGAGAGATTTGAAAAAA
GGTGAAGCTTTTTCTTTGCCAAGGATAAAGCTTTCTAGGATTGATGTTGA
ATGGGTTCATGTACTTAGTGAAGGATGGGCTACACCACTTGGTGGGTTCA
TGAGAGAAAGAGAGTTCCTCCAAACGCTTCATTTCAATTCACTTAGACTC
GATGATGGGTCTGTGGTGAATATGTCTGTTCCGATTGTTTTGGCCATTGA
TGATGTGCAGAAACATCGGATCGGTGATTCAAAAAAAGTTGCACTTTTTG
ATTCACTGGGTAACCCTGTTGCTATTCTGAATGATATTGAAATTTATAAG
CACCCTAAAGAAGAAAGGATAGCCCGAACTTGGGGAACAACAGCCCCTGG
CCTACCTTAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0000103	sulfate assimilation

Vocabulary: **Molecular Function**
Term	Definition
GO:0004781	sulfate adenylyltransferase (ATP) activity

Vocabulary: **INTERPRO**
Term	Definition
IPR002650	Sulphate_adenylyltransferase
IPR015947	PUA-like_sf

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