GR398034

EST Overview


Name	GR398034
Unique Name	GR398034
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	765

Libraries


Library Name	Type
LIBEST_024782 ICC4958 field drought stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR398034 vs. SwissProt
Match: C98A2_SOYBN (Cytochrome P450 98A2 OS=Glycine max GN=CYP98A2 PE=2 SV=1)

HSP 1 Score: 382.104 bits (980), Expect = 2.000e-115
Identity = 181/207 (87.44%), Postives = 199/207 (96.14%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            MAL L IP+SL+TL+L YT+YQ+L+FKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSN+ELAKE+LKE+DQ LADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELF+PKRLEALRPIREDEVT+MV+S++N  T+ ENLGKGIL+RKH+G VAFNNITRLAFGKRFVNSEGVMDEQG+EFK
Sbjct:    1 MALLLIIPISLVTLWLGYTLYQRLRFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNSELAKEVLKEHDQLLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFSPKRLEALRPIREDEVTSMVDSVYNHCTSTENLGKGILLRKHLGVVAFNNITRLAFGKRFVNSEGVMDEQGVEFK 207

HSP 2 Score: 55.8398 bits (133), Expect = 2.000e-115
Identity = 24/26 (92.31%), Postives = 25/26 (96.15%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+V NGLKLGASLAMAEHIPWLRWMF
Sbjct:  208 AIVENGLKLGASLAMAEHIPWLRWMF 233

[Close]

BLAST of GR398034 vs. SwissProt
Match: C98A3_ARATH (Cytochrome P450 98A3 OS=Arabidopsis thaliana GN=CYP98A3 PE=1 SV=1)

HSP 1 Score: 320.472 bits (820), Expect = 1.348e-96
Identity = 152/207 (73.43%), Postives = 180/207 (86.96%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            M+ FL I ++ I   + Y + Q+L++K PPGP P P+VGNLYDIKPVRFRC+ EWAQSYGPIISVW GS LNV+VS+ ELAKE+LKE+DQ+LADRHR+RS   FSR+G+DLIWADYGPHYVKVRKVCTLELFTPKRLE+LRPIREDEVTAMVES+F D   PEN  KG+ +RK++GAVAFNNITRLAFGKRF+N+EGV+DEQG+EFK
Sbjct:    1 MSWFL-IAVATIAAVVSYKLIQRLRYKFPPGPSPKPIVGNLYDIKPVRFRCYYEWAQSYGPIISVWIGSILNVVVSSAELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVCTLELFTPKRLESLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLAFGKRFMNAEGVVDEQGLEFK 206

HSP 2 Score: 54.6842 bits (130), Expect = 1.348e-96
Identity = 23/26 (88.46%), Postives = 26/26 (100.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+VSNGLKLGASL++AEHIPWLRWMF
Sbjct:  207 AIVSNGLKLGASLSIAEHIPWLRWMF 232

[Close]

BLAST of GR398034 vs. SwissProt
Match: C98A1_SORBI (Cytochrome P450 98A1 OS=Sorghum bicolor GN=CYP98A1 PE=2 SV=1)

HSP 1 Score: 293.893 bits (751), Expect = 5.645e-83
Identity = 138/207 (66.67%), Postives = 171/207 (82.61%), Query Frame = 1

Query:   70 ALFLTIPLSLITLFLCYTIYQKLKF-KLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            +L L++ L+++ + L   +  +L+  +LPPGPRPWPV+GNL  IKP+R RCF EWA+ YGP+ISVWFGS L V+VS +ELAKE+LKENDQQLADR R+RS  +FSR+G+DLIWADYGPHY+KVRK+C LELFTPKRLEALRPIREDEVTAMVES++   T P N GK +++R H+  VAFNNITRLAFGKRF+N+ G +DEQG EFK
Sbjct:    4 SLLLSVALAVVLIPLSLALLNRLRLGRLPPGPRPWPVLGNLRQIKPIRCRCFQEWAERYGPVISVWFGSGLTVVVSTSELAKEVLKENDQQLADRPRNRSTQRFSRNGQDLIWADYGPHYIKVRKLCNLELFTPKRLEALRPIREDEVTAMVESVYRAATAPGNEGKPMVVRNHLSMVAFNNITRLAFGKRFMNANGDIDEQGREFK 210

HSP 2 Score: 35.8094 bits (81), Expect = 5.645e-83
Identity = 14/24 (58.33%), Postives = 22/24 (91.67%), Query Frame = 3

Query:  690 VVSNGLKLGASLAMAEHIPWLRWM 761
            +V+NG+K+GASL++AE I +LRW+
Sbjct:  212 IVNNGIKIGASLSVAEFIWYLRWL 235

[Close]

BLAST of GR398034 vs. SwissProt
Match: C75A1_PINTA (Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 4.000e-28
Identity = 69/204 (33.82%), Postives = 118/204 (57.84%), Query Frame = 1

Query:   79 LTIPLSLITLFL----CYTIYQKLKF-KLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQG 675
            L +P  LI  F+    C+ ++Q  +  +LPPGP PWP++GN + ++    R     A+ YGPI+ + FGS   V+VS++E AK  LK +D   A R  +     F  + KD+ ++ YG H+ K+RK+C LEL T KR+E+ + +R++E++AM+ SI+ ++   E+    + + K I     N + R+   K+F +++   D +G
Sbjct:   14 LPLPAILIATFIFFFSCWILHQSQRNERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPILFLRFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMRKICVLELLTSKRIESFKHVRQEELSAMIHSIWEES---ESGRIAVNVSKAISTSLANILWRILARKKFSDNDLGADGKG 214

[Close]

BLAST of GR398034 vs. SwissProt
Match: F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 3.744e-26
Identity = 63/207 (30.43%), Postives = 111/207 (53.62%), Query Frame = 1

Query:   73 LFLTIPLSLITLFLCYTIYQK-LKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKR-FVNSEGVMDEQGIEFK 687
            +  T+  S +  F+  + ++K     LPPGP+PWP++GNL  + P   +  A  AQ+YGP++ +  G    V+ ++  +A + LK +D   + R  +  A   + + +DL++A YGP +  +RK+C++ LF+ K L+  R +R+DEV  +  ++ +    P  LG      + +     N + R+  GKR F +  G +D Q  EFK
Sbjct:    7 ILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAMAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLG------QLLNVCTTNALARVMLGKRVFADGSGDVDPQAAEFK 207

[Close]

BLAST of GR398034 vs. SwissProt
Match: C83A1_ARATH (Cytochrome P450 83A1 OS=Arabidopsis thaliana GN=CYP83A1 PE=1 SV=2)

HSP 1 Score: 118.242 bits (295), Expect = 4.890e-26
Identity = 71/194 (36.60%), Postives = 105/194 (54.12%), Query Frame = 1

Query:   85 IPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIK---PVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEG 657
            + L+ + LF  Y   +  ++KLPPGP P PV+GNL  ++   P RF  FA WA+ YGPI+S   GS   V++S+ ELAKE+LK  D   ADR   R     S   +D+    Y P+Y ++RK+    LF+P R+   + +RE+E   M++ I       E +    LM     +V    + R AFGK++ N +G
Sbjct:    9 VALAAVLLFFLYQKPKTKRYKLPPGPSPLPVIGNLLQLQKLNPQRF--FAGWAKKYGPILSYRIGSRTMVVISSAELAKELLKTQDVNFADRPPHRGHEFISYGRRDMALNHYTPYYREIRKMGMNHLFSPTRVATFKHVREEEARRMMDKINKAADKSEVVDISELMLTFTNSV----VCRQAFGKKY-NEDG 195

[Close]

BLAST of GR398034 vs. SwissProt
Match: C71BZ_ARATH (Cytochrome P450 71B38 OS=Arabidopsis thaliana GN=CYP71B38 PE=2 SV=2)

HSP 1 Score: 112.849 bits (281), Expect = 2.054e-24
Identity = 65/195 (33.33%), Postives = 115/195 (58.97%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKL---KFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRF 642
            +   L +PLSLI       +++KL   K KLPPGP   P++GNL+ +  + ++ F + +Q YGP++ +  G    ++VS+ E A+E+LK +D +   R ++ +   F+ + KD+ +A +G  + ++RK+ TLELF+ K+L++ R IRE+E   +V+ I       +N    + +RK + +   + I RLAFG+ F
Sbjct:    5 LCFLLLLPLSLI-------LFKKLLPSKGKLPPGPIGLPIIGNLHQLGKLLYKSFHKISQEYGPVVLLRLGVVPVIVVSSKEGAEEVLKTHDLETCTRPKTAATGLFTYNFKDIGFAPFGDDWREMRKITTLELFSVKKLKSFRYIREEESELLVKKISKSVDETQN--SSVDLRKVLFSFTASIICRLAFGQNF 190

[Close]

BLAST of GR398034 vs. SwissProt
Match: C71B2_ARATH (Cytochrome P450 71B2 OS=Arabidopsis thaliana GN=CYP71B2 PE=2 SV=2)

HSP 1 Score: 112.849 bits (281), Expect = 2.054e-24
Identity = 67/211 (31.75%), Postives = 119/211 (56.40%), Query Frame = 1

Query:   58 TFSMALFLTIPLSLIT-LFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDT--TNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIE 681
            T  +  FL   L++++ +FL     +  KF LPP P   P++GNL+ +  +  RCF + +  YGP++ +  GS   V++S++E A+ +LK ND +   R ++  + K S   KD+ +A YG ++ +VRK+  +ELF+ K++++ R IRE+EV  +V+ +       +P +L K         ++  + I R+A G+ F  S  V+D+  IE
Sbjct:    2 TILLCFFLVSLLTIVSSIFLKQN--KTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYGPLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWREVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFF------SLTASIICRVALGQNFNESGFVIDQDRIE 204

[Close]

BLAST of GR398034 vs. SwissProt
Match: C71BL_ARATH (Cytochrome P450 71B21 OS=Arabidopsis thaliana GN=CYP71B21 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 2.271e-23
Identity = 68/195 (34.87%), Postives = 115/195 (58.97%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKL---KFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRF 642
            M++FL   L L+ LFL +  Y++L   K KLPPGP   P++GNL+ +     R F + +Q YGP++ + FG    V+ S  E A+E+LK +D +   R +  +   F+ + KD+ +A YG  + ++RK+  LELF+ K+L+A R IRE+E   +V+ +  ++   + L   + +RK + +   + + RLAFG+ F
Sbjct:    1 MSIFLCF-LLLLPLFLVF--YKRLLPSKGKLPPGPISLPIIGNLHQLGKSLHRSFYKLSQEYGPVMFLRFGVVPVVVFSTKEAAEEVLKTHDLETCTRPKLSATGLFTYNFKDIGFAQYGEDWREMRKLAMLELFSSKKLKAFRYIREEESELLVKKV-TESAQTQTL---VDLRKALFSYTASIVCRLAFGQNF 188

[Close]

BLAST of GR398034 vs. SwissProt
Match: C71BM_ARATH (Cytochrome P450 71B22 OS=Arabidopsis thaliana GN=CYP71B22 PE=2 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 2.967e-23
Identity = 67/187 (35.83%), Postives = 108/187 (57.75%), Query Frame = 1

Query:   91 LSLITLFLCYTIYQKL---KFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRF 642
            L L+ LFL +  ++KL   K KLPPGP   P++GNL+ +     R F + +Q+YGP++ + FG    V+VS  E A+E+LK +D +   R +  +   FS + KD+ +A YG  + ++RK+  LELF+ K+L+A R IRE+E   +V    N  +        + +RK + +   + + RLAFG+ F
Sbjct:    8 LLLLPLFLIF--FKKLSPSKGKLPPGPLGLPIIGNLHQLGKSLHRSFHKLSQNYGPVMFLHFGVVPVVVVSTREAAEEVLKTHDLETCTRPKLTATKLFSYNYKDIGFAQYGDDWREMRKLAMLELFSSKKLKAFRYIREEESEVLV----NKLSKSAETRTMVDLRKALFSYTASIVCRLAFGQNF 188

[Close]

BLAST of GR398034 vs. TrEMBL
Match: D0EYH3_TRIPR (p-coumaroyl-shikimate 3'-hydroxylase OS=Trifolium pratense GN=CYP98A44v2 PE=2 SV=1)

HSP 1 Score: 398.282 bits (1022), Expect = 3.290e-119
Identity = 191/207 (92.27%), Postives = 204/207 (98.55%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            MALFLTIPLSLIT+FL YT++Q+L+FKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSN+ELAKE+LKE DQQLADRHRSRSAAKFSRDG+DLIWADYGPHYVKVRKVCTLELF+PKR+EALRPIREDEVTAMVESIFND+TN ENLGKGILMRK+IGAVAFNNITRLAFGKRFVNSEGVMDEQG+EFK
Sbjct:    1 MALFLTIPLSLITIFLFYTLFQRLRFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNSELAKEVLKEKDQQLADRHRSRSAAKFSRDGQDLIWADYGPHYVKVRKVCTLELFSPKRIEALRPIREDEVTAMVESIFNDSTNSENLGKGILMRKYIGAVAFNNITRLAFGKRFVNSEGVMDEQGVEFK 207

HSP 2 Score: 56.225 bits (134), Expect = 3.290e-119
Identity = 24/26 (92.31%), Postives = 26/26 (100.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+V+NGLKLGASLAMAEHIPWLRWMF
Sbjct:  208 AIVANGLKLGASLAMAEHIPWLRWMF 233

[Close]

BLAST of GR398034 vs. TrEMBL
Match: C9EGT6_TRIPR (p-coumaroyl-shikimate 3'-hydroxylase OS=Trifolium pratense GN=CYP98A44 PE=2 SV=1)

HSP 1 Score: 397.897 bits (1021), Expect = 4.293e-119
Identity = 190/207 (91.79%), Postives = 204/207 (98.55%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            MALFLTIPLSLIT+FL YT++Q+L+FKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVI+SN+ELAKE+LKE DQQLADRHRSRSAAKFSRDG+DLIWADYGPHYVKVRKVCTLELF+PKR+EALRPIREDEVTAMVESIFND+TN ENLGKGILMRK+IGAVAFNNITRLAFGKRFVNSEGVMDEQG+EFK
Sbjct:    1 MALFLTIPLSLITIFLFYTLFQRLRFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIISNSELAKEVLKEKDQQLADRHRSRSAAKFSRDGQDLIWADYGPHYVKVRKVCTLELFSPKRIEALRPIREDEVTAMVESIFNDSTNSENLGKGILMRKYIGAVAFNNITRLAFGKRFVNSEGVMDEQGVEFK 207

HSP 2 Score: 56.225 bits (134), Expect = 4.293e-119
Identity = 24/26 (92.31%), Postives = 26/26 (100.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+V+NGLKLGASLAMAEHIPWLRWMF
Sbjct:  208 AIVANGLKLGASLAMAEHIPWLRWMF 233

[Close]

BLAST of GR398034 vs. TrEMBL
Match: Q2MJ10_MEDTR (Cytochrome P450 monooxygenase CYP98A37 OS=Medicago truncatula GN=CYP98A37 PE=2 SV=1)

HSP 1 Score: 395.586 bits (1015), Expect = 2.119e-118
Identity = 189/207 (91.30%), Postives = 203/207 (98.07%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            MALFLTIPLS I +FL YT++Q+L+FKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSN++LAKE+LKENDQQLADRHRSRSAAKFSRDG+DLIWADYGPHYVKVRKVCTLELF+PKR+EALRPIREDEVTAMVESIFND+TN ENLGKGILMRK+IGAVAFNNITRLAFGKRFVNSEGVMDEQG+EFK
Sbjct:    1 MALFLTIPLSFIAIFLFYTLFQRLRFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNSKLAKEVLKENDQQLADRHRSRSAAKFSRDGQDLIWADYGPHYVKVRKVCTLELFSPKRIEALRPIREDEVTAMVESIFNDSTNSENLGKGILMRKYIGAVAFNNITRLAFGKRFVNSEGVMDEQGVEFK 207

HSP 2 Score: 56.225 bits (134), Expect = 2.119e-118
Identity = 24/26 (92.31%), Postives = 26/26 (100.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+V+NGLKLGASLAMAEHIPWLRWMF
Sbjct:  208 AIVANGLKLGASLAMAEHIPWLRWMF 233

[Close]

BLAST of GR398034 vs. TrEMBL
Match: B2Z6P3_POPTR (Coumaroyl 3-hydroxylase OS=Populus trichocarpa GN=CYP98A27 PE=2 SV=1)

HSP 1 Score: 376.326 bits (965), Expect = 5.716e-113
Identity = 178/203 (87.68%), Postives = 193/203 (95.07%), Query Frame = 1

Query:   79 LTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            L IP+S ITL L Y IYQ+L+FKLPPGPRPWP+VGNLYD+KPVRFRCFAEWAQ+YGPIISVWFGSTLNVIVSNTELAKE+LKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELF+PKRLEALRPIREDEV AMVESIFND TNPEN GK + ++K++GAVAFNNITRLAFGKRFVN+EGVMDEQG+EFK
Sbjct:    4 LLIPISFITLLLTYKIYQRLRFKLPPGPRPWPIVGNLYDVKPVRFRCFAEWAQAYGPIISVWFGSTLNVIVSNTELAKEVLKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFSPKRLEALRPIREDEVAAMVESIFNDCTNPENNGKTLTVKKYLGAVAFNNITRLAFGKRFVNAEGVMDEQGLEFK 206

HSP 2 Score: 57.3806 bits (137), Expect = 5.716e-113
Identity = 25/26 (96.15%), Postives = 26/26 (100.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+VSNGLKLGASLAMAEHIPWLRWMF
Sbjct:  207 AIVSNGLKLGASLAMAEHIPWLRWMF 232

[Close]

BLAST of GR398034 vs. TrEMBL
Match: B0LXE6_9ROSI (p-coumaryl-CoA 3'-hydroxylase OS=Populus alba x Populus grandidentata PE=2 SV=1)

HSP 1 Score: 376.326 bits (965), Expect = 5.716e-113
Identity = 177/203 (87.19%), Postives = 194/203 (95.57%), Query Frame = 1

Query:   79 LTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            L IP+S IT+ L Y IYQ+L+FKLPPGPRPWP+VGNLYD+KPVRFRCFAEWAQ+YGPIISVWFGSTLNVIVSNTELAKE+LKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELF+PKRLEALRPIREDEVTAMVESIFND TNPEN GK ++++K++GAVAFNNITRLAFGKRF N+EGVMDEQG+EFK
Sbjct:    4 LLIPISFITILLTYKIYQRLRFKLPPGPRPWPIVGNLYDVKPVRFRCFAEWAQAYGPIISVWFGSTLNVIVSNTELAKEVLKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFSPKRLEALRPIREDEVTAMVESIFNDCTNPENNGKTLMVKKYLGAVAFNNITRLAFGKRFENAEGVMDEQGLEFK 206

HSP 2 Score: 57.3806 bits (137), Expect = 5.716e-113
Identity = 25/26 (96.15%), Postives = 26/26 (100.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+VSNGLKLGASLAMAEHIPWLRWMF
Sbjct:  207 AIVSNGLKLGASLAMAEHIPWLRWMF 232

[Close]

BLAST of GR398034 vs. TrEMBL
Match: B9SJN4_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_1106600 PE=3 SV=1)

HSP 1 Score: 372.089 bits (954), Expect = 2.370e-111
Identity = 175/205 (85.37%), Postives = 194/205 (94.63%), Query Frame = 1

Query:   73 LFLTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            L +TIP+S++ L L Y +Y +L+FKLPPGPRPWP+VGNLYDIKPVRFRCFAEWAQ+YGPIISVWFGSTLNVIVSNTELAKE+LKE+DQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRL+ALRPIREDEVTAMVESIF D TNPEN GK +L++K++GAVAFNNITRLAFGKRFVN+EG+MDEQG EFK
Sbjct:    5 LIVTIPISILLLILTYKLYYRLRFKLPPGPRPWPIVGNLYDIKPVRFRCFAEWAQAYGPIISVWFGSTLNVIVSNTELAKEVLKEHDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLDALRPIREDEVTAMVESIFMDCTNPENNGKSVLVKKYLGAVAFNNITRLAFGKRFVNAEGIMDEQGKEFK 209

HSP 2 Score: 56.225 bits (134), Expect = 2.370e-111
Identity = 24/26 (92.31%), Postives = 26/26 (100.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+V+NGLKLGASLAMAEHIPWLRWMF
Sbjct:  210 AIVANGLKLGASLAMAEHIPWLRWMF 235

[Close]

BLAST of GR398034 vs. TrEMBL
Match: D6QY03_EUCGL (4-coumarate 3-hydroxylase OS=Eucalyptus globulus PE=3 SV=1)

HSP 1 Score: 357.066 bits (915), Expect = 3.418e-107
Identity = 169/205 (82.44%), Postives = 193/205 (94.15%), Query Frame = 1

Query:   73 LFLTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            + L+IPL  + L L + +YQ+L+FKLPPGPR WPVVGNLYDIKPVRFRCFAEW+Q+YGPIISVWFGSTLNV+VS++ELAKE+LKENDQQLADRHRSRSAAKFSRDG+DLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIF D TNP+N GK +L++K++GAVAFNNITRLAFGKRF+N+EGV+DEQG+EFK
Sbjct:    6 ILLSIPL--LFLLLAHQLYQRLRFKLPPGPRAWPVVGNLYDIKPVRFRCFAEWSQAYGPIISVWFGSTLNVVVSSSELAKEVLKENDQQLADRHRSRSAAKFSRDGQDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFKDCTNPDNSGKTLLVKKYLGAVAFNNITRLAFGKRFMNAEGVIDEQGLEFK 208

HSP 2 Score: 57.3806 bits (137), Expect = 3.418e-107
Identity = 25/26 (96.15%), Postives = 26/26 (100.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+VSNGLKLGASLAMAEHIPWLRWMF
Sbjct:  209 AIVSNGLKLGASLAMAEHIPWLRWMF 234

[Close]

BLAST of GR398034 vs. TrEMBL
Match: A9ZT63_COPJA (Cytochrome P450 OS=Coptis japonica var. dissecta GN=CYP98A46 PE=2 SV=1)

HSP 1 Score: 351.288 bits (900), Expect = 4.104e-105
Identity = 163/199 (81.91%), Postives = 183/199 (91.96%), Query Frame = 1

Query:   91 LSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            L LI   + Y +YQ+L+FKLPPGPRPWP+VGNLYDIKPVRFRCFAEWAQ+YGPI+SVWFGS LNV+VSN+ELAKE+LKENDQQLADRHRSRSAAKFSRDG DLIWADYGPHYVKVRKVCTLELF+PKR+E+LRPIREDEVTAMVESIFN  T PEN GK ++++ ++  VAFNNITRLAFGKRFVNSEGVMDEQG+EFK
Sbjct:   11 LILILFIVAYKLYQRLRFKLPPGPRPWPIVGNLYDIKPVRFRCFAEWAQTYGPIMSVWFGSILNVVVSNSELAKEVLKENDQQLADRHRSRSAAKFSRDGTDLIWADYGPHYVKVRKVCTLELFSPKRIESLRPIREDEVTAMVESIFNHCTKPENYGKSLVVKNYLSTVAFNNITRLAFGKRFVNSEGVMDEQGVEFK 209

HSP 2 Score: 56.225 bits (134), Expect = 4.104e-105
Identity = 24/26 (92.31%), Postives = 26/26 (100.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+V+NGLKLGASLAMAEHIPWLRWMF
Sbjct:  210 AIVANGLKLGASLAMAEHIPWLRWMF 235

[Close]

BLAST of GR398034 vs. TrEMBL
Match: Q8L5H7_OCIBA (P-coumaroyl shikimate 3'-hydroxylase isoform 2 OS=Ocimum basilicum PE=2 SV=1)

HSP 1 Score: 350.132 bits (897), Expect = 4.104e-105
Identity = 166/207 (80.19%), Postives = 190/207 (91.79%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            MA  L + L L  +FL + +Y +L+F+LPPGPRP PVVGNLYD+KPVRFRCFA+WAQSYGPIISVWFGSTLNVIVSNTELAKE+LKE DQQLADRHRSRSAAKFSRDG+DLIWADYGPHYVKVRKVC LELF+PKRLEALRPIREDEVTAMVESI++D T P+N GK +L++K++GAVAFNNITRLAFGKRFVNSEG++D+QG+EFK
Sbjct:    1 MAALLLLLLLLPAIFLLHHLYYRLRFRLPPGPRPLPVVGNLYDVKPVRFRCFADWAQSYGPIISVWFGSTLNVIVSNTELAKEVLKEKDQQLADRHRSRSAAKFSRDGQDLIWADYGPHYVKVRKVCMLELFSPKRLEALRPIREDEVTAMVESIYHDCTAPDNAGKSLLVKKYLGAVAFNNITRLAFGKRFVNSEGIIDKQGLEFK 207

HSP 2 Score: 57.3806 bits (137), Expect = 4.104e-105
Identity = 25/26 (96.15%), Postives = 26/26 (100.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+VSNGLKLGASLAMAEHIPWLRWMF
Sbjct:  208 AIVSNGLKLGASLAMAEHIPWLRWMF 233

[Close]

BLAST of GR398034 vs. TrEMBL
Match: Q8L5H8_OCIBA (P-coumaroyl shikimate 3'-hydroxylase isoform 1 OS=Ocimum basilicum PE=2 SV=1)

HSP 1 Score: 355.14 bits (910), Expect = 5.354e-105
Identity = 166/208 (79.81%), Postives = 193/208 (92.79%), Query Frame = 1

Query:   64 SMALFLTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            ++ L L +PL L  +FL + +Y +L+F+LPPGPRP P+VGNLYD+KPVRFRCFA+WAQSYGPIISVWFGSTLNVIVSNTELAKE+LKE DQQLADRHRSRSAAKFSRDG+DLIWADYGPHYVKVRKVCTLELF+PKRLEALRPIREDEVTAMVESI++D T P+N GK +L++K++GAVAFNNITRLAFGKRFVNSEG++D+QG+EFK
Sbjct:    3 ALLLLLLLPLLLPAIFLLHHLYYRLRFRLPPGPRPLPIVGNLYDVKPVRFRCFADWAQSYGPIISVWFGSTLNVIVSNTELAKEVLKEKDQQLADRHRSRSAAKFSRDGQDLIWADYGPHYVKVRKVCTLELFSPKRLEALRPIREDEVTAMVESIYHDCTAPDNAGKSLLVKKYLGAVAFNNITRLAFGKRFVNSEGIIDKQGLEFK 210

HSP 2 Score: 51.9878 bits (123), Expect = 5.354e-105
Identity = 24/26 (92.31%), Postives = 25/26 (96.15%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+VSNGLKLGASLAMAEHIP LRWMF
Sbjct:  211 AIVSNGLKLGASLAMAEHIPSLRWMF 236

[Close]

BLAST of GR398034 vs. TAIR peptide
Match: AT2G40890.1 (| Symbols: CYP98A3 | cytochrome P450, family 98, subfamily A, polypeptide 3 | chr2:17058291-17060532 REVERSE LENGTH=508)

HSP 1 Score: 320.472 bits (820), Expect = 1.347e-97
Identity = 152/207 (73.43%), Postives = 180/207 (86.96%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIEFK 687
            M+ FL I ++ I   + Y + Q+L++K PPGP P P+VGNLYDIKPVRFRC+ EWAQSYGPIISVW GS LNV+VS+ ELAKE+LKE+DQ+LADRHR+RS   FSR+G+DLIWADYGPHYVKVRKVCTLELFTPKRLE+LRPIREDEVTAMVES+F D   PEN  KG+ +RK++GAVAFNNITRLAFGKRF+N+EGV+DEQG+EFK
Sbjct:    1 MSWFL-IAVATIAAVVSYKLIQRLRYKFPPGPSPKPIVGNLYDIKPVRFRCYYEWAQSYGPIISVWIGSILNVVVSSAELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVCTLELFTPKRLESLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLAFGKRFMNAEGVVDEQGLEFK 206

HSP 2 Score: 54.6842 bits (130), Expect = 1.347e-97
Identity = 23/26 (88.46%), Postives = 26/26 (100.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWMF 764
            A+VSNGLKLGASL++AEHIPWLRWMF
Sbjct:  207 AIVSNGLKLGASLSIAEHIPWLRWMF 232

[Close]

BLAST of GR398034 vs. TAIR peptide
Match: AT1G74550.1 (| Symbols: CYP98A9 | cytochrome P450, family 98, subfamily A, polypeptide 9 | chr1:28016086-28017549 FORWARD LENGTH=487)

HSP 1 Score: 177.948 bits (450), Expect = 5.199e-45
Identity = 88/193 (45.60%), Postives = 135/193 (69.95%), Query Frame = 1

Query:   79 LTIPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEG 657
            L I L+ I +       ++    +PPGP    +VGNL+ +KP+  + F+EW+Q+YGPIISVW GS L V+VS+++LAK++L++ D QL +RHR+   A+ +++G DLIW+DYG HYVK+RK+CTLELF+ K +E  R +RE EV++MV+SIFND  + +   K +++R ++ +VA N ++RL  GK F   +G
Sbjct:    4 LLISLTTIIIAAYMQNLRRRGSNIPPGPPTRFLVGNLHQLKPLWTQSFSEWSQTYGPIISVWLGSQLAVVVSSSDLAKQVLRDKDYQLCNRHRT---ARMTQNGSDLIWSDYGAHYVKMRKLCTLELFSLKSIECFRSMREMEVSSMVKSIFNDFMSDDQ--KPVVLRNYLDSVALNIVSRLVIGKTFEPKDG 191

[Close]

BLAST of GR398034 vs. TAIR peptide
Match: AT1G74540.1 (| Symbols: CYP98A8 | cytochrome P450, family 98, subfamily A, polypeptide 8 | chr1:28013362-28014855 FORWARD LENGTH=497)

HSP 1 Score: 176.792 bits (447), Expect = 6.518e-45
Identity = 88/199 (44.22%), Postives = 135/199 (67.84%), Query Frame = 1

Query:   73 LFLTIPLSLITLFLCYTIYQKL-KFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFND---TTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEG 657
            L   +P+ + TL L    YQ+  +  +PPGP+P  ++GNL+ +KP+    F+EW+++YGPIISVW GS L V+VS+++LA+++L++ D QL++RHR    A+ ++ G DL+W+DY PHYVK+RK+CTLELF+ K +E  R +RE E  +MV SI  D    +  +   K +++RK++ AV  N I+RL  GK F + EG
Sbjct:    5 LISLLPIIVATLML----YQRWWRSNIPPGPKPKFLLGNLHQMKPLWTHSFSEWSETYGPIISVWIGSQLTVVVSSSDLARQVLRDKDHQLSNRHR---IARMTQTGTDLVWSDYSPHYVKLRKLCTLELFSLKSIENFRSLREMEARSMVVSILKDLMSNSGDDQERKPVIVRKYLAAVVLNTISRLMIGKEFGSEEG 196

HSP 2 Score: 22.3274 bits (46), Expect = 6.518e-45
Identity = 8/25 (32.00%), Postives = 14/25 (56.00%), Query Frame = 3

Query:  687 AVVSNGLKLGASLAMAEHIPWLRWM 761
            A+V     L  S  + +H+ WL+W+
Sbjct:  201 AIVEKEHLLSGSGTILDHVWWLKWV 225

[Close]

BLAST of GR398034 vs. TAIR peptide
Match: AT4G13770.1 (| Symbols: CYP83A1, REF2 | cytochrome P450, family 83, subfamily A, polypeptide 1 | chr4:7990682-7992282 REVERSE LENGTH=502)

HSP 1 Score: 118.242 bits (295), Expect = 4.889e-27
Identity = 71/194 (36.60%), Postives = 105/194 (54.12%), Query Frame = 1

Query:   85 IPLSLITLFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIK---PVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEG 657
            + L+ + LF  Y   +  ++KLPPGP P PV+GNL  ++   P RF  FA WA+ YGPI+S   GS   V++S+ ELAKE+LK  D   ADR   R     S   +D+    Y P+Y ++RK+    LF+P R+   + +RE+E   M++ I       E +    LM     +V    + R AFGK++ N +G
Sbjct:    9 VALAAVLLFFLYQKPKTKRYKLPPGPSPLPVIGNLLQLQKLNPQRF--FAGWAKKYGPILSYRIGSRTMVVISSAELAKELLKTQDVNFADRPPHRGHEFISYGRRDMALNHYTPYYREIRKMGMNHLFSPTRVATFKHVREEEARRMMDKINKAADKSEVVDISELMLTFTNSV----VCRQAFGKKY-NEDG 195

[Close]

BLAST of GR398034 vs. TAIR peptide
Match: AT3G44250.1 (| Symbols: CYP71B38 | cytochrome P450, family 71, subfamily B, polypeptide 38 | chr3:15948505-15950224 REVERSE LENGTH=499)

HSP 1 Score: 112.849 bits (281), Expect = 2.054e-25
Identity = 65/195 (33.33%), Postives = 115/195 (58.97%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKL---KFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRF 642
            +   L +PLSLI       +++KL   K KLPPGP   P++GNL+ +  + ++ F + +Q YGP++ +  G    ++VS+ E A+E+LK +D +   R ++ +   F+ + KD+ +A +G  + ++RK+ TLELF+ K+L++ R IRE+E   +V+ I       +N    + +RK + +   + I RLAFG+ F
Sbjct:    5 LCFLLLLPLSLI-------LFKKLLPSKGKLPPGPIGLPIIGNLHQLGKLLYKSFHKISQEYGPVVLLRLGVVPVIVVSSKEGAEEVLKTHDLETCTRPKTAATGLFTYNFKDIGFAPFGDDWREMRKITTLELFSVKKLKSFRYIREEESELLVKKISKSVDETQN--SSVDLRKVLFSFTASIICRLAFGQNF 190

[Close]

BLAST of GR398034 vs. TAIR peptide
Match: AT1G13080.1 (| Symbols: CYP71B2 | cytochrome P450, family 71, subfamily B, polypeptide 2 | chr1:4459212-4460807 FORWARD LENGTH=502)

HSP 1 Score: 112.849 bits (281), Expect = 2.054e-25
Identity = 67/211 (31.75%), Postives = 119/211 (56.40%), Query Frame = 1

Query:   58 TFSMALFLTIPLSLIT-LFLCYTIYQKLKFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDT--TNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMDEQGIE 681
            T  +  FL   L++++ +FL     +  KF LPP P   P++GNL+ +  +  RCF + +  YGP++ +  GS   V++S++E A+ +LK ND +   R ++  + K S   KD+ +A YG ++ +VRK+  +ELF+ K++++ R IRE+EV  +V+ +       +P +L K         ++  + I R+A G+ F  S  V+D+  IE
Sbjct:    2 TILLCFFLVSLLTIVSSIFLKQN--KTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYGPLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWREVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFF------SLTASIICRVALGQNFNESGFVIDQDRIE 204

[Close]

BLAST of GR398034 vs. TAIR peptide
Match: AT3G26190.1 (| Symbols: CYP71B21 | cytochrome P450, family 71, subfamily B, polypeptide 21 | chr3:9583475-9585083 REVERSE LENGTH=499)

HSP 1 Score: 109.383 bits (272), Expect = 2.271e-24
Identity = 68/195 (34.87%), Postives = 115/195 (58.97%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKL---KFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRF 642
            M++FL   L L+ LFL +  Y++L   K KLPPGP   P++GNL+ +     R F + +Q YGP++ + FG    V+ S  E A+E+LK +D +   R +  +   F+ + KD+ +A YG  + ++RK+  LELF+ K+L+A R IRE+E   +V+ +  ++   + L   + +RK + +   + + RLAFG+ F
Sbjct:    1 MSIFLCF-LLLLPLFLVF--YKRLLPSKGKLPPGPISLPIIGNLHQLGKSLHRSFYKLSQEYGPVMFLRFGVVPVVVFSTKEAAEEVLKTHDLETCTRPKLSATGLFTYNFKDIGFAQYGEDWREMRKLAMLELFSSKKLKAFRYIREEESELLVKKV-TESAQTQTL---VDLRKALFSYTASIVCRLAFGQNF 188

[Close]

BLAST of GR398034 vs. TAIR peptide
Match: AT3G26200.1 (| Symbols: CYP71B22 | cytochrome P450, family 71, subfamily B, polypeptide 22 | chr3:9589347-9590972 FORWARD LENGTH=500)

HSP 1 Score: 108.997 bits (271), Expect = 2.966e-24
Identity = 67/187 (35.83%), Postives = 108/187 (57.75%), Query Frame = 1

Query:   91 LSLITLFLCYTIYQKL---KFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRF 642
            L L+ LFL +  ++KL   K KLPPGP   P++GNL+ +     R F + +Q+YGP++ + FG    V+VS  E A+E+LK +D +   R +  +   FS + KD+ +A YG  + ++RK+  LELF+ K+L+A R IRE+E   +V    N  +        + +RK + +   + + RLAFG+ F
Sbjct:    8 LLLLPLFLIF--FKKLSPSKGKLPPGPLGLPIIGNLHQLGKSLHRSFHKLSQNYGPVMFLHFGVVPVVVVSTREAAEEVLKTHDLETCTRPKLTATKLFSYNYKDIGFAQYGDDWREMRKLAMLELFSSKKLKAFRYIREEESEVLV----NKLSKSAETRTMVDLRKALFSYTASIVCRLAFGQNF 188

[Close]

BLAST of GR398034 vs. TAIR peptide
Match: AT3G53280.1 (| Symbols: CYP71B5 | cytochrome p450 71b5 | chr3:19755749-19757466 FORWARD LENGTH=498)

HSP 1 Score: 107.842 bits (268), Expect = 6.608e-24
Identity = 67/195 (34.36%), Postives = 109/195 (55.90%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKL---KFKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIFNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRF 642
            +   L +PLSLI L       +KL   K KLPPGP+  P++GNL+       +   + +Q YGP++ + FG    +IVS+ E A+E+LK +D +   R ++  +  F+ + KD+ +A YG ++ ++RK+   ELF+ K+L++ R IREDE   +V  +       E     + +RK I   A + I RL+FG+ F
Sbjct:    5 LCFLLLLPLSLIFL-------KKLLPSKGKLPPGPKGLPIIGNLHQFGRFLHKSLHKISQEYGPVMLLHFGVVPVIIVSSKEGAEEVLKTHDLETCSRPKTVGSGLFTYNFKDIGFAPYGENWREMRKIAVSELFSQKKLKSFRYIREDESQLLVRKVSKSAL--ETPTSSVNLRKVIFTFAASIICRLSFGQNF 190

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BLAST of GR398034 vs. TAIR peptide
Match: AT2G30770.1 (| Symbols: CYP71A13 | cytochrome P450, family 71, subfamily A, polypeptide 13 | chr2:13109909-13112006 REVERSE LENGTH=503)

HSP 1 Score: 106.686 bits (265), Expect = 1.472e-23
Identity = 64/207 (30.92%), Postives = 111/207 (53.62%), Query Frame = 1

Query:   67 MALFLTIPLSLITLFLCYTIYQKLK-----FKLPPGPRPWPVVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVSNTELAKEILKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESI--FNDTTNPENLGKGILMRKHIGAVAFNNITRLAFGKRFVNSEGVMD 666
            M + L+I L L TL     + + LK       LPP P   PV+GNL+ +     R     +  YGP++ + FG    ++VS+ E A+E+LK +D + A+R RS++       G+D+++A YG ++ +++ VC L L T K +E+   +REDEV AM+E +   + +++ ENL +  +       +  +  +R+A G++    E   D
Sbjct:    7 MEMILSISLCLTTLITLLLLRRFLKRTATKVNLPPSPWRLPVIGNLHQLSLHPHRSLRSLSLRYGPLMLLHFGRVPILVVSSGEAAQEVLKTHDHKFANRPRSKAVHGLMNGGRDVVFAPYGEYWRQMKSVCILNLLTNKMVESFEKVREDEVNAMIEKLEKASSSSSSENLSELFI------TLPSDVTSRVALGRKHSEDETARD 207

The following BLAST results are available for this feature:

BLAST of GR398034 vs. SwissProt
BLAST of GR398034 vs. TrEMBL
BLAST of GR398034 vs. TAIR peptide

BLAST of GR398034 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
C98A2_SOYBN	2.000e-115	87.44	Cytochrome P450 98A2 OS=Glycine max GN=CYP98A2 PE=...	[more]
C98A3_ARATH	1.348e-96	73.43	Cytochrome P450 98A3 OS=Arabidopsis thaliana GN=CY...	[more]
C98A1_SORBI	5.645e-83	66.67	Cytochrome P450 98A1 OS=Sorghum bicolor GN=CYP98A1...	[more]
C75A1_PINTA	4.000e-28	33.82	Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 P...	[more]
F3PH_PETHY	3.744e-26	30.43	Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=C...	[more]
C83A1_ARATH	4.890e-26	36.60	Cytochrome P450 83A1 OS=Arabidopsis thaliana GN=CY...	[more]
C71BZ_ARATH	2.054e-24	33.33	Cytochrome P450 71B38 OS=Arabidopsis thaliana GN=C...	[more]
C71B2_ARATH	2.054e-24	31.75	Cytochrome P450 71B2 OS=Arabidopsis thaliana GN=CY...	[more]
C71BL_ARATH	2.271e-23	34.87	Cytochrome P450 71B21 OS=Arabidopsis thaliana GN=C...	[more]
C71BM_ARATH	2.967e-23	35.83	Cytochrome P450 71B22 OS=Arabidopsis thaliana GN=C...	[more]

BLAST of GR398034 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D0EYH3_TRIPR	3.290e-119	92.27	p-coumaroyl-shikimate 3'-hydroxylase OS=Trifolium ...	[more]
C9EGT6_TRIPR	4.293e-119	91.79	p-coumaroyl-shikimate 3'-hydroxylase OS=Trifolium ...	[more]
Q2MJ10_MEDTR	2.119e-118	91.30	Cytochrome P450 monooxygenase CYP98A37 OS=Medicago...	[more]
B2Z6P3_POPTR	5.716e-113	87.68	Coumaroyl 3-hydroxylase OS=Populus trichocarpa GN=...	[more]
B0LXE6_9ROSI	5.716e-113	87.19	p-coumaryl-CoA 3'-hydroxylase OS=Populus alba x Po...	[more]
B9SJN4_RICCO	2.370e-111	85.37	Cytochrome P450, putative OS=Ricinus communis GN=R...	[more]
D6QY03_EUCGL	3.418e-107	82.44	4-coumarate 3-hydroxylase OS=Eucalyptus globulus P...	[more]
A9ZT63_COPJA	4.104e-105	81.91	Cytochrome P450 OS=Coptis japonica var. dissecta G...	[more]
Q8L5H7_OCIBA	4.104e-105	80.19	P-coumaroyl shikimate 3'-hydroxylase isoform 2 OS=...	[more]
Q8L5H8_OCIBA	5.354e-105	79.81	P-coumaroyl shikimate 3'-hydroxylase isoform 1 OS=...	[more]

BLAST of GR398034 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT2G40890.1	1.347e-97	73.43	\| Symbols: CYP98A3 \| cytochrome P450, family 98, s...	[more]
AT1G74550.1	5.199e-45	45.60	\| Symbols: CYP98A9 \| cytochrome P450, family 98, s...	[more]
AT1G74540.1	6.518e-45	44.22	\| Symbols: CYP98A8 \| cytochrome P450, family 98, s...	[more]
AT4G13770.1	4.889e-27	36.60	\| Symbols: CYP83A1, REF2 \| cytochrome P450, family...	[more]
AT3G44250.1	2.054e-25	33.33	\| Symbols: CYP71B38 \| cytochrome P450, family 71, ...	[more]
AT1G13080.1	2.054e-25	31.75	\| Symbols: CYP71B2 \| cytochrome P450, family 71, s...	[more]
AT3G26190.1	2.271e-24	34.87	\| Symbols: CYP71B21 \| cytochrome P450, family 71, ...	[more]
AT3G26200.1	2.966e-24	35.83	\| Symbols: CYP71B22 \| cytochrome P450, family 71, ...	[more]
AT3G53280.1	6.608e-24	34.36	\| Symbols: CYP71B5 \| cytochrome p450 71b5 \| chr3:1...	[more]
AT2G30770.1	1.472e-23	30.92	\| Symbols: CYP71A13 \| cytochrome P450, family 71, ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	1.10.630.10	no description	coord: 50..218 score: 3.2
IPR001128	Cytochrome P450	PANTHER	PTHR19383	CYTOCHROME P450	coord: 20..229 score: 3.6e
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 51..215 score: 5.6
IPR001128	Cytochrome P450	SUPERFAMILY	48264	Cytochrome P450	coord: 48..214 score: 5.1
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 78..97 score: 3e-08coord: 102..123 score: 3e-08coord: 200..218 score: 3
None	No IPR available	PANTHER	PTHR19383:SF44	CYTOCHROME P450, SUBFAMILY 98A	coord: 20..229 score: 3.6e

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR398034 ID=GR398034; Name=GR398034; organism=Cicer arietinum; type=EST; length=765bp
GGTGTAACATATATGAAGATCAACCTGTTTCCAACCTTCTCTAATCAATC
AAACACAACTTTCTCAATGGCTCTGTTTCTTACAATTCCCCTTTCACTCA
TCACCCTTTTCCTTTGTTACACAATCTACCAAAAACTCAAATTCAAACTT
CCACCCGGTCCACGCCCGTGGCCAGTGGTTGGAAACCTCTATGACATAAA
ACCGGTCCGGTTCCGGTGTTTTGCAGAATGGGCCCAATCTTATGGGCCCA
TTATATCAGTTTGGTTTGGTTCGACTTTAAACGTTATTGTTTCCAATACA
GAATTGGCGAAAGAGATATTAAAGGAGAACGATCAACAATTGGCTGACCG
ACACAGGAGTCGGTCAGCTGCGAAATTTAGTAGAGATGGGAAGGATTTAA
TTTGGGCCGATTATGGGCCGCATTATGTGAAAGTGAGGAAGGTTTGTACG
TTGGAGCTTTTTACACCTAAGAGACTTGAAGCTTTAAGACCTATTCGAGA
AGATGAGGTTACTGCCATGGTTGAATCCATTTTCAATGACACAACCAATC
CTGAGAATTTGGGGAAAGGTATATTGATGAGGAAACATATAGGGGCAGTG
GCATTCAACAACATCACTAGGTTGGCATTTGGGAAAAGATTTGTGAACTC
AGAAGGTGTAATGGATGAGCAAGGAATAGAATTTAAGCTGTAGTATCAAA
TGGGTTAAAGCTAGGAGCATCTCTAGCTATGGCAGAGCATATCCCTTGGC
TGCGCTGGATGTTCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0009055	electron transfer activity
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR002401	Cyt_P450_E_grp-I

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