FD449514

EST Overview


Name	FD449514
Unique Name	FD449514
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	353

Libraries


Library Name	Type
LIBEST_022499 Chickpea Ascochyta-Induced Library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FD449514 vs. SwissProt
Match: DCE2_ARATH (Glutamate decarboxylase 2 OS=Arabidopsis thaliana GN=GAD2 PE=1 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 3.980e-37
Identity = 72/100 (72.00%), Postives = 84/100 (84.00%), Query Frame = -3

Query:   22 EERNEKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIK 321
            +E  EKT RFNI+SKD GVP+VAF+LKD S H  FEI++ LR++GWIVPAYTMPADAQHI VLRVVIREDFS +LAERLV+DI KV+  LDTLPS +S K
Sbjct:  354 KEGIEKTERFNIVSKDQGVPVVAFSLKDHSFHNEFEISEMLRRFGWIVPAYTMPADAQHITVLRVVIREDFSRTLAERLVADISKVLHELDTLPSKISKK 453

HSP 2 Score: 31.5722 bits (70), Expect = 3.980e-37
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = -1

Query:  297 MENCLENTIVLKKGMRKQE 353
            MENC+EN +VLK+G+ K E
Sbjct:  343 MENCIENMVVLKEGIEKTE 361

[Close]

BLAST of FD449514 vs. SwissProt
Match: DCE1_ARATH (Glutamate decarboxylase 1 OS=Arabidopsis thaliana GN=GAD1 PE=1 SV=2)

HSP 1 Score: 142.51 bits (358), Expect = 1.944e-36
Identity = 72/93 (77.42%), Postives = 81/93 (87.10%), Query Frame = -3

Query:   31 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTV 309
            EKT RFNI+SKD GVPLVAF+LKDSS HT FEI+D LR+YGWIVPAYTMP +AQHI VLRVVIREDFS +LAERLV DI+KV++ LD LPS V
Sbjct:  359 EKTERFNIVSKDEGVPLVAFSLKDSSCHTEFEISDMLRRYGWIVPAYTMPPNAQHITVLRVVIREDFSRTLAERLVIDIEKVMRELDELPSRV 451

HSP 2 Score: 29.6462 bits (65), Expect = 1.944e-36
Identity = 12/19 (63.16%), Postives = 15/19 (78.95%), Query Frame = -1

Query:  297 MENCLENTIVLKKGMRKQE 353
            MENC EN IVL++G+ K E
Sbjct:  344 MENCRENMIVLREGLEKTE 362

[Close]

BLAST of FD449514 vs. SwissProt
Match: DCE_SOLLC (Glutamate decarboxylase OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 3.298e-36
Identity = 71/101 (70.30%), Postives = 88/101 (87.13%), Query Frame = -3

Query:    4 KTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIKSAHVTA 306
            K GRF+I+SKDVGVP+VAF+L+DSS +TVFE+++HLR++GWIVPAYTMP DA+HIAVLRVVIREDFS SLAERLVSDI+K++  LDT P  +  K+  VTA
Sbjct:  361 KMGRFDIVSKDVGVPVVAFSLRDSSKYTVFEVSEHLRRFGWIVPAYTMPPDAEHIAVLRVVIREDFSHSLAERLVSDIEKILSELDTQPPRLPTKAVRVTA 461

HSP 2 Score: 22.7126 bits (47), Expect = 3.298e-36
Identity = 9/17 (52.94%), Postives = 12/17 (70.59%), Query Frame = -1

Query:  303 MENCLENTIVLKKGMRK 353
            M+NCL N  VL +G+ K
Sbjct:  345 MKNCLSNAKVLTEGITK 361

[Close]

BLAST of FD449514 vs. SwissProt
Match: DCE_PETHY (Glutamate decarboxylase OS=Petunia hybrida GN=GAD PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.098e-35
Identity = 69/98 (70.41%), Postives = 84/98 (85.71%), Query Frame = -3

Query:   16 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIKSA 309
            EKTGRFNI+SK++GVPLVAF+LKD+  H  FEI++ LR++GWIVPAYTMP +AQHI VLRVVIREDFS +LAERLV DI+KV+  LDTLP+ V+ K A
Sbjct:  359 EKTGRFNIISKEIGVPLVAFSLKDNRQHNEFEISETLRRFGWIVPAYTMPPNAQHITVLRVVIREDFSRTLAERLVRDIEKVLHELDTLPARVNAKLA 456

HSP 2 Score: 26.1794 bits (56), Expect = 2.098e-35
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = -1

Query:  303 MENCLENTIVLKKGMRK 353
            MENC EN  VL++G+ K
Sbjct:  344 MENCQENASVLREGLEK 360

[Close]

BLAST of FD449514 vs. SwissProt
Match: DCE_LACLM (Glutamate decarboxylase OS=Lactococcus lactis subsp. cremoris (strain MG1363) GN=gadB PE=1 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 5.875e-11
Identity = 32/92 (34.78%), Postives = 56/92 (60.87%), Query Frame = -3

Query:   49 ERNEKTGRFNILSKDVGVPLVAFTLKDSSHH--TVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLD 318
            E  EKTG F I++    +P+V + LK++S+    ++++AD L   GW VPAY +P + ++  + R+VIR DF  ++A   V D+ + +  L+
Sbjct:  356 EEIEKTGMFEIMNDGAQLPIVCYKLKENSNRGWNLYDLADRLLMKGWQVPAYPLPKNLENEIIQRLVIRADFGMNMAFNYVQDMQEAIDALN 447

[Close]

BLAST of FD449514 vs. SwissProt
Match: DCE_LACLA (Glutamate decarboxylase OS=Lactococcus lactis subsp. lactis GN=gadB PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.309e-10
Identity = 31/89 (34.83%), Postives = 55/89 (61.80%), Query Frame = -3

Query:   49 EKTGRFNILSKDVGVPLVAFTLKDSSHH--TVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLD 309
            EKTG F I++    +P+V + LK+ S+    ++++AD L   GW VPAY +P + ++  + R+VIR DF  ++A   V D+ + ++ L+
Sbjct:  359 EKTGMFEIMNDGSQLPIVCYKLKEDSNRGWNLYDLADRLLMKGWQVPAYPLPKNLENEIIQRLVIRADFGMNMAFNYVQDMQEAIEALN 447

[Close]

BLAST of FD449514 vs. SwissProt
Match: DCEB_LISMO (Glutamate decarboxylase beta OS=Listeria monocytogenes GN=gadB PE=3 SV=2)

HSP 1 Score: 64.3142 bits (155), Expect = 2.233e-10
Identity = 30/89 (33.71%), Postives = 53/89 (59.55%), Query Frame = -3

Query:   49 EKTGRFNILSKDVGVPLVAFTLKDSSH--HTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLD 309
            E+TG F+I +    +P+V + LKD ++   T++++AD L+  GW VPAY +P   ++I + R V R D   ++AE  + D    ++ L+
Sbjct:  357 EQTGYFDIFNDGSHLPIVCYKLKDDANVNWTLYDLADRLQMRGWQVPAYPLPKSLENIIIQRYVCRADLGFNMAEEFIQDFQASIQELN 445

[Close]

BLAST of FD449514 vs. SwissProt
Match: DCEB_LISIN (Glutamate decarboxylase beta OS=Listeria innocua GN=gadB PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.233e-10
Identity = 30/89 (33.71%), Postives = 54/89 (60.67%), Query Frame = -3

Query:   49 EKTGRFNILSKDVGVPLVAFTLKDSSH--HTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLD 309
            E+TG F+I +    +P+V + LKD ++   T++++AD L+  GW VPAY +P + ++I + R V R D   ++AE  + D    ++ L+
Sbjct:  357 EQTGYFDIYNDGSHLPIVCYKLKDDANVKWTLYDLADRLQMRGWQVPAYPLPKNLENIIIQRYVCRADLGFNMAEEFIQDFQASIQELN 445

[Close]

BLAST of FD449514 vs. SwissProt
Match: DCEB_SHIFL (Glutamate decarboxylase beta OS=Shigella flexneri GN=gadB PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.108e-9
Identity = 31/86 (36.05%), Postives = 48/86 (55.81%), Query Frame = -3

Query:   25 DVGVPLVAFTLKDSSH--HTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSI 276
            D G+P V F LKD     +T++++++ LR  GW VPA+T+  +A  I V+R++ R  F    AE L+ D    +K+L   P    I
Sbjct:  372 DEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGI 457

[Close]

BLAST of FD449514 vs. SwissProt
Match: DCEB_ECOLI (Glutamate decarboxylase beta OS=Escherichia coli (strain K12) GN=gadB PE=1 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.108e-9
Identity = 31/86 (36.05%), Postives = 48/86 (55.81%), Query Frame = -3

Query:   25 DVGVPLVAFTLKDSSH--HTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSI 276
            D G+P V F LKD     +T++++++ LR  GW VPA+T+  +A  I V+R++ R  F    AE L+ D    +K+L   P    I
Sbjct:  372 DEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGI 457

[Close]

BLAST of FD449514 vs. TrEMBL
Match: C6TCI6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 3.780e-42
Identity = 83/106 (78.30%), Postives = 95/106 (89.62%), Query Frame = -3

Query:    4 EERNEKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIKSAHVTA 321
            +E  E+TGRFNI+SKD+GVPLVAF+LKDSS HTVFEIADHLRK+GWIVPAYTMP DAQHIAVLRVVIREDFS  LAERL +DI+KVVK LDTLPS ++ K+ H+TA
Sbjct:  358 KEGIERTGRFNIISKDIGVPLVAFSLKDSSQHTVFEIADHLRKFGWIVPAYTMPPDAQHIAVLRVVIREDFSRGLAERLAADIEKVVKLLDTLPSPLTTKAVHITA 463

HSP 2 Score: 25.409 bits (54), Expect = 3.780e-42
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = -1

Query:  303 MENCLENTIVLKKGMRK 353
            MENC EN  VLK+G+ +
Sbjct:  347 MENCWENARVLKEGIER 363

[Close]

BLAST of FD449514 vs. TrEMBL
Match: B9I4W8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_569405 PE=3 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 2.536e-37
Identity = 72/100 (72.00%), Postives = 91/100 (91.00%), Query Frame = -3

Query:   10 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIKSAHV 309
            EKTGRF+I+SKD GVPLVAF+LKDSS HTVFEIA+ LR++GWI+PAYTMPA+AQHIAVLRVV+REDF+ SLAERLVS ID+V+K  D+LPS V+++++ +
Sbjct:  359 EKTGRFDIVSKDKGVPLVAFSLKDSSKHTVFEIAESLRRFGWIIPAYTMPANAQHIAVLRVVVREDFNRSLAERLVSHIDQVMKETDSLPSRVAVQASRI 458

HSP 2 Score: 28.1054 bits (61), Expect = 2.536e-37
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = -1

Query:  303 MENCLENTIVLKKGMRK 353
            MENCLEN  VL++G+ K
Sbjct:  344 MENCLENARVLREGLEK 360

[Close]

BLAST of FD449514 vs. TrEMBL
Match: B9S7C3_RICCO (Glutamate decarboxylase, putative OS=Ricinus communis GN=RCOM_0776380 PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 3.620e-36
Identity = 75/107 (70.09%), Postives = 89/107 (83.18%), Query Frame = -3

Query:    1 EERNEKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIKSAHVTAS 321
            +E  EKTGRFNI+SKD+GVPLVAF+LKD+S H  FEIA+ LR++GWIVPAYTMPADAQHI VLRVVIREDFS +LAERLV DI KV+  LD LP+ +S K +  TA+
Sbjct:   33 KEGLEKTGRFNIVSKDIGVPLVAFSLKDNSRHNEFEIAETLRRFGWIVPAYTMPADAQHITVLRVVIREDFSRTLAERLVLDITKVLHELDQLPAKISAKVSVSTAA 139

HSP 2 Score: 26.9498 bits (58), Expect = 3.620e-36
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = -1

Query:  303 MENCLENTIVLKKGMRK 353
            MENC +N +VLK+G+ K
Sbjct:   22 MENCHQNAMVLKEGLEK 38

[Close]

BLAST of FD449514 vs. TrEMBL
Match: Q8RXH0_ARATH (Glutamate decarboxylase, putative OS=Arabidopsis thaliana GN=At1g65960 PE=2 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 7.908e-36
Identity = 72/100 (72.00%), Postives = 84/100 (84.00%), Query Frame = -3

Query:   22 EERNEKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIK 321
            +E  EKT RFNI+SKD GVP+VAF+LKD S H  FEI++ LR++GWIVPAYTMPADAQHI VLRVVIREDFS +LAERLV+DI KV+  LDTLPS +S K
Sbjct:  279 KEGIEKTERFNIVSKDQGVPVVAFSLKDHSFHNEFEISEMLRRFGWIVPAYTMPADAQHITVLRVVIREDFSRTLAERLVADISKVLHELDTLPSKISKK 378

HSP 2 Score: 31.5722 bits (70), Expect = 7.908e-36
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = -1

Query:  297 MENCLENTIVLKKGMRKQE 353
            MENC+EN +VLK+G+ K E
Sbjct:  268 MENCIENMVVLKEGIEKTE 286

[Close]

BLAST of FD449514 vs. TrEMBL
Match: Q56W28_ARATH (Putative uncharacterized protein At1g65960 OS=Arabidopsis thaliana GN=At1g65960 PE=2 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 7.921e-36
Identity = 72/100 (72.00%), Postives = 84/100 (84.00%), Query Frame = -3

Query:   22 EERNEKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIK 321
            +E  EKT RFNI+SKD GVP+VAF+LKD S H  FEI++ LR++GWIVPAYTMPADAQHI VLRVVIREDFS +LAERLV+DI KV+  LDTLPS +S K
Sbjct:  225 KEGIEKTERFNIVSKDQGVPVVAFSLKDHSFHNEFEISEMLRRFGWIVPAYTMPADAQHITVLRVVIREDFSRTLAERLVADISKVLHELDTLPSKISKK 324

HSP 2 Score: 31.5722 bits (70), Expect = 7.921e-36
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = -1

Query:  297 MENCLENTIVLKKGMRKQE 353
            MENC+EN +VLK+G+ K E
Sbjct:  214 MENCIENMVVLKEGIEKTE 232

[Close]

BLAST of FD449514 vs. TrEMBL
Match: D7LWZ5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_488599 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.744e-35
Identity = 71/93 (76.34%), Postives = 81/93 (87.10%), Query Frame = -3

Query:   31 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTV 309
            EKT RFNI+SKD GVPLVAF+LKD+S HT FEI+D LR+YGWIVPAYTMP +AQHI VLRVVIREDFS +LAERLV DI+KV++ LD LPS V
Sbjct:  359 EKTERFNIVSKDEGVPLVAFSLKDNSSHTEFEISDMLRRYGWIVPAYTMPPNAQHITVLRVVIREDFSRTLAERLVIDIEKVMRELDELPSRV 451

HSP 2 Score: 31.187 bits (69), Expect = 1.744e-35
Identity = 13/19 (68.42%), Postives = 15/19 (78.95%), Query Frame = -1

Query:  297 MENCLENTIVLKKGMRKQE 353
            MENC EN IVL+KG+ K E
Sbjct:  344 MENCRENMIVLRKGLEKTE 362

[Close]

BLAST of FD449514 vs. TrEMBL
Match: Q944L6_ARATH (At1g65960/F12P19_12 OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 2.272e-35
Identity = 71/100 (71.00%), Postives = 83/100 (83.00%), Query Frame = -3

Query:   22 EERNEKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIK 321
            +E  EKT RFNI+SKD GVP+VAF+LKD S H  FEI++ LR++GWIVPAYTMPAD QHI VLRVVIREDFS +LAERLV+DI KV+  LDTLPS +S K
Sbjct:  354 KEGIEKTERFNIVSKDQGVPVVAFSLKDHSFHNEFEISEMLRRFGWIVPAYTMPADVQHITVLRVVIREDFSRTLAERLVADISKVLHELDTLPSKISKK 453

HSP 2 Score: 31.5722 bits (70), Expect = 2.272e-35
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = -1

Query:  297 MENCLENTIVLKKGMRKQE 353
            MENC+EN +VLK+G+ K E
Sbjct:  343 MENCIENMVVLKEGIEKTE 361

[Close]

BLAST of FD449514 vs. TrEMBL
Match: Q6Q4I2_BRAJU (Glutamate decarboxylase 4a OS=Brassica juncea GN=GAD4a PE=2 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 2.272e-35
Identity = 72/103 (69.90%), Postives = 87/103 (84.47%), Query Frame = -3

Query:    1 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIKSAHVTAS 309
            EKTGRFNI+SK+ GVPLVAF+LKDSS H  FE+A+ LR++GWIVPAYTMPADAQH+ VLRVVIREDFS +LAERLV+D +KV+  LDTLP+ V  K A+  A+
Sbjct:  359 EKTGRFNIVSKENGVPLVAFSLKDSSRHDEFEVAETLRRFGWIVPAYTMPADAQHVTVLRVVIREDFSRTLAERLVADFEKVLHELDTLPARVQAKMANGNAN 461

HSP 2 Score: 25.7942 bits (55), Expect = 2.272e-35
Identity = 9/17 (52.94%), Postives = 14/17 (82.35%), Query Frame = -1

Query:  303 MENCLENTIVLKKGMRK 353
            M+NC EN +VL++G+ K
Sbjct:  344 MDNCRENMMVLREGLEK 360

[Close]

BLAST of FD449514 vs. TrEMBL
Match: Q94KK8_TOBAC (Glutamate decarboxylase isozyme 3 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 2.960e-35
Identity = 71/100 (71.00%), Postives = 84/100 (84.00%), Query Frame = -3

Query:   10 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIKSAHV 309
            EKTGRFNI+SKD GVPLVAF+LKD+S H  FE+++ LR++GWIVPAYTMPADAQH+ VLRVVIREDFS +LAERLV DI KV+  LDTLP+ +S K   V
Sbjct:  359 EKTGRFNIVSKDEGVPLVAFSLKDNSRHNEFEVSETLRRFGWIVPAYTMPADAQHVTVLRVVIREDFSRTLAERLVLDIVKVLHELDTLPARLSAKLEEV 458

HSP 2 Score: 28.4906 bits (62), Expect = 2.960e-35
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = -1

Query:  303 MENCLENTIVLKKGMRK 353
            MENC EN IVL++G+ K
Sbjct:  344 MENCRENAIVLREGLEK 360

[Close]

BLAST of FD449514 vs. TrEMBL
Match: Q6Q4I3_BRAJU (Glutamate decarboxylase 2 OS=Brassica juncea GN=GAD2 PE=2 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 3.856e-35
Identity = 72/96 (75.00%), Postives = 83/96 (86.46%), Query Frame = -3

Query:   22 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIK 309
            EKT RFNI+SK+VGVPLVAF+LKD S H  FEI++ LR++GWIVPAYTMPADAQHI VLRVVIREDFS +LAERLV+DI KV+  LDTLPS +S K
Sbjct:  359 EKTERFNIVSKEVGVPLVAFSLKDHSFHNEFEISEMLRRFGWIVPAYTMPADAQHITVLRVVIREDFSRTLAERLVADIVKVLHELDTLPSKISKK 454

HSP 2 Score: 28.8758 bits (63), Expect = 3.856e-35
Identity = 11/19 (57.89%), Postives = 15/19 (78.95%), Query Frame = -1

Query:  297 MENCLENTIVLKKGMRKQE 353
            MENC EN +VL++G+ K E
Sbjct:  344 MENCRENMVVLREGIEKTE 362

[Close]

BLAST of FD449514 vs. TAIR peptide
Match: AT1G65960.2 (| Symbols: GAD2 | glutamate decarboxylase 2 | chr1:24552094-24557253 FORWARD LENGTH=494)

HSP 1 Score: 142.895 bits (359), Expect = 3.064e-38
Identity = 72/100 (72.00%), Postives = 84/100 (84.00%), Query Frame = -3

Query:   22 EERNEKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIK 321
            +E  EKT RFNI+SKD GVP+VAF+LKD S H  FEI++ LR++GWIVPAYTMPADAQHI VLRVVIREDFS +LAERLV+DI KV+  LDTLPS +S K
Sbjct:  354 KEGIEKTERFNIVSKDQGVPVVAFSLKDHSFHNEFEISEMLRRFGWIVPAYTMPADAQHITVLRVVIREDFSRTLAERLVADISKVLHELDTLPSKISKK 453

HSP 2 Score: 31.5722 bits (70), Expect = 3.064e-38
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = -1

Query:  297 MENCLENTIVLKKGMRKQE 353
            MENC+EN +VLK+G+ K E
Sbjct:  343 MENCIENMVVLKEGIEKTE 361

[Close]

BLAST of FD449514 vs. TAIR peptide
Match: AT1G65960.1 (| Symbols: GAD2 | glutamate decarboxylase 2 | chr1:24555868-24557253 FORWARD LENGTH=365)

HSP 1 Score: 142.895 bits (359), Expect = 3.076e-38
Identity = 72/100 (72.00%), Postives = 84/100 (84.00%), Query Frame = -3

Query:   22 EERNEKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIK 321
            +E  EKT RFNI+SKD GVP+VAF+LKD S H  FEI++ LR++GWIVPAYTMPADAQHI VLRVVIREDFS +LAERLV+DI KV+  LDTLPS +S K
Sbjct:  225 KEGIEKTERFNIVSKDQGVPVVAFSLKDHSFHNEFEISEMLRRFGWIVPAYTMPADAQHITVLRVVIREDFSRTLAERLVADISKVLHELDTLPSKISKK 324

HSP 2 Score: 31.5722 bits (70), Expect = 3.076e-38
Identity = 12/19 (63.16%), Postives = 16/19 (84.21%), Query Frame = -1

Query:  297 MENCLENTIVLKKGMRKQE 353
            MENC+EN +VLK+G+ K E
Sbjct:  214 MENCIENMVVLKEGIEKTE 232

[Close]

BLAST of FD449514 vs. TAIR peptide
Match: AT5G17330.1 (| Symbols: GAD, GAD1 | glutamate decarboxylase | chr5:5711141-5714839 FORWARD LENGTH=502)

HSP 1 Score: 142.51 bits (358), Expect = 1.497e-37
Identity = 72/93 (77.42%), Postives = 81/93 (87.10%), Query Frame = -3

Query:   31 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTV 309
            EKT RFNI+SKD GVPLVAF+LKDSS HT FEI+D LR+YGWIVPAYTMP +AQHI VLRVVIREDFS +LAERLV DI+KV++ LD LPS V
Sbjct:  359 EKTERFNIVSKDEGVPLVAFSLKDSSCHTEFEISDMLRRYGWIVPAYTMPPNAQHITVLRVVIREDFSRTLAERLVIDIEKVMRELDELPSRV 451

HSP 2 Score: 29.6462 bits (65), Expect = 1.497e-37
Identity = 12/19 (63.16%), Postives = 15/19 (78.95%), Query Frame = -1

Query:  297 MENCLENTIVLKKGMRKQE 353
            MENC EN IVL++G+ K E
Sbjct:  344 MENCRENMIVLREGLEKTE 362

[Close]

BLAST of FD449514 vs. TAIR peptide
Match: AT2G02000.1 (| Symbols: GAD3 | glutamate decarboxylase 3 | chr2:469505-471997 REVERSE LENGTH=500)

HSP 1 Score: 145.976 bits (367), Expect = 1.950e-37
Identity = 71/98 (72.45%), Postives = 84/98 (85.71%), Query Frame = -3

Query:   16 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIKSA 309
            EKTGRFNI+SK+ GVPLVAF+LKDSS H  FE+A+ LR++GWIVPAYTMPADAQH+ VLRVVIREDFS +LAERLV+D +KV+  LDTLP+ V  K A
Sbjct:  359 EKTGRFNIVSKENGVPLVAFSLKDSSRHNEFEVAEMLRRFGWIVPAYTMPADAQHVTVLRVVIREDFSRTLAERLVADFEKVLHELDTLPARVHAKMA 456

HSP 2 Score: 25.7942 bits (55), Expect = 1.950e-37
Identity = 9/17 (52.94%), Postives = 14/17 (82.35%), Query Frame = -1

Query:  303 MENCLENTIVLKKGMRK 353
            M+NC EN +VL++G+ K
Sbjct:  344 MDNCRENMMVLRQGLEK 360

[Close]

BLAST of FD449514 vs. TAIR peptide
Match: AT2G02010.1 (| Symbols: GAD4 | glutamate decarboxylase 4 | chr2:474375-476495 REVERSE LENGTH=493)

HSP 1 Score: 142.895 bits (359), Expect = 1.616e-36
Identity = 70/99 (70.71%), Postives = 83/99 (83.84%), Query Frame = -3

Query:   13 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIKSAH 309
            EKTGRF I+SK+ GVPLVAF+LKDSS H  FE+A  LR++GWIVPAYTMPADAQH+ VLRVVIREDFS +LAERLV+D +KV+  LDTLP+ V  K A+
Sbjct:  359 EKTGRFKIVSKENGVPLVAFSLKDSSRHNEFEVAHTLRRFGWIVPAYTMPADAQHVTVLRVVIREDFSRTLAERLVADFEKVLHELDTLPARVHAKMAN 457

HSP 2 Score: 25.7942 bits (55), Expect = 1.616e-36
Identity = 9/17 (52.94%), Postives = 14/17 (82.35%), Query Frame = -1

Query:  303 MENCLENTIVLKKGMRK 353
            M+NC EN +VL++G+ K
Sbjct:  344 MDNCRENMMVLRQGLEK 360

[Close]

BLAST of FD449514 vs. TAIR peptide
Match: AT3G17760.2 (| Symbols: GAD5 | glutamate decarboxylase 5 | chr3:6078893-6080838 REVERSE LENGTH=494)

HSP 1 Score: 144.05 bits (362), Expect = 1.706e-35
Identity = 71/103 (68.93%), Postives = 88/103 (85.44%), Query Frame = -3

Query:    1 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIKSAHVTAS 309
            E TG+FNI+SKD+GVPLVAF+LKDSS HTVFEIA+ LRK+GWI+PAYTMPADAQHIAVLRVVIREDFS  LA+RL++ I +V+K ++ LPS +    AH+ A+
Sbjct:  358 EMTGKFNIVSKDIGVPLVAFSLKDSSKHTVFEIAESLRKFGWIIPAYTMPADAQHIAVLRVVIREDFSRGLADRLITHIIQVLKEIEGLPSRI----AHLAAA 456

[Close]

BLAST of FD449514 vs. TAIR peptide
Match: AT3G17760.1 (| Symbols: GAD5 | glutamate decarboxylase 5 | chr3:6078893-6080838 REVERSE LENGTH=494)

HSP 1 Score: 144.05 bits (362), Expect = 1.706e-35
Identity = 71/103 (68.93%), Postives = 88/103 (85.44%), Query Frame = -3

Query:    1 EKTGRFNILSKDVGVPLVAFTLKDSSHHTVFEIADHLRKYGWIVPAYTMPADAQHIAVLRVVIREDFSCSLAERLVSDIDKVVKHLDTLPSTVSIKSAHVTAS 309
            E TG+FNI+SKD+GVPLVAF+LKDSS HTVFEIA+ LRK+GWI+PAYTMPADAQHIAVLRVVIREDFS  LA+RL++ I +V+K ++ LPS +    AH+ A+
Sbjct:  358 EMTGKFNIVSKDIGVPLVAFSLKDSSKHTVFEIAESLRKFGWIIPAYTMPADAQHIAVLRVVIREDFSRGLADRLITHIIQVLKEIEGLPSRI----AHLAAA 456

The following BLAST results are available for this feature:

BLAST of FD449514 vs. SwissProt
BLAST of FD449514 vs. TrEMBL
BLAST of FD449514 vs. TAIR peptide

BLAST of FD449514 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
DCE2_ARATH	3.980e-37	72.00	Glutamate decarboxylase 2 OS=Arabidopsis thaliana ...	[more]
DCE1_ARATH	1.944e-36	77.42	Glutamate decarboxylase 1 OS=Arabidopsis thaliana ...	[more]
DCE_SOLLC	3.298e-36	70.30	Glutamate decarboxylase OS=Solanum lycopersicum PE...	[more]
DCE_PETHY	2.098e-35	70.41	Glutamate decarboxylase OS=Petunia hybrida GN=GAD ...	[more]
DCE_LACLM	5.875e-11	34.78	Glutamate decarboxylase OS=Lactococcus lactis subs...	[more]
DCE_LACLA	1.309e-10	34.83	Glutamate decarboxylase OS=Lactococcus lactis subs...	[more]
DCEB_LISMO	2.233e-10	33.71	Glutamate decarboxylase beta OS=Listeria monocytog...	[more]
DCEB_LISIN	2.233e-10	33.71	Glutamate decarboxylase beta OS=Listeria innocua G...	[more]
DCEB_SHIFL	1.108e-9	36.05	Glutamate decarboxylase beta OS=Shigella flexneri ...	[more]
DCEB_ECOLI	1.108e-9	36.05	Glutamate decarboxylase beta OS=Escherichia coli (...	[more]

BLAST of FD449514 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TCI6_SOYBN	3.780e-42	78.30	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9I4W8_POPTR	2.536e-37	72.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9S7C3_RICCO	3.620e-36	70.09	Glutamate decarboxylase, putative OS=Ricinus commu...	[more]
Q8RXH0_ARATH	7.908e-36	72.00	Glutamate decarboxylase, putative OS=Arabidopsis t...	[more]
Q56W28_ARATH	7.921e-36	72.00	Putative uncharacterized protein At1g65960 OS=Arab...	[more]
D7LWZ5_ARALY	1.744e-35	76.34	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q944L6_ARATH	2.272e-35	71.00	At1g65960/F12P19_12 OS=Arabidopsis thaliana PE=2 S...	[more]
Q6Q4I2_BRAJU	2.272e-35	69.90	Glutamate decarboxylase 4a OS=Brassica juncea GN=G...	[more]
Q94KK8_TOBAC	2.960e-35	71.00	Glutamate decarboxylase isozyme 3 OS=Nicotiana tab...	[more]
Q6Q4I3_BRAJU	3.856e-35	75.00	Glutamate decarboxylase 2 OS=Brassica juncea GN=GA...	[more]

BLAST of FD449514 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT1G65960.2	3.064e-38	72.00	\| Symbols: GAD2 \| glutamate decarboxylase 2 \| chr1...	[more]
AT1G65960.1	3.076e-38	72.00	\| Symbols: GAD2 \| glutamate decarboxylase 2 \| chr1...	[more]
AT5G17330.1	1.497e-37	77.42	\| Symbols: GAD, GAD1 \| glutamate decarboxylase \| c...	[more]
AT2G02000.1	1.950e-37	72.45	\| Symbols: GAD3 \| glutamate decarboxylase 3 \| chr2...	[more]
AT2G02010.1	1.616e-36	70.71	\| Symbols: GAD4 \| glutamate decarboxylase 4 \| chr2...	[more]
AT3G17760.2	1.706e-35	68.93	\| Symbols: GAD5 \| glutamate decarboxylase 5 \| chr3...	[more]
AT3G17760.1	1.706e-35	68.93	\| Symbols: GAD5 \| glutamate decarboxylase 5 \| chr3...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002129	Pyridoxal phosphate-dependent decarboxylase	PANTHER	PTHR11999	GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE	coord: 15..107 score: 4.9
IPR010107	Glutamate decarboxylase	PANTHER	PTHR11999:SF1	GLUTAMATE DECARBOXYLASE	coord: 15..107 score: 4.9
IPR015424	Pyridoxal phosphate-dependent transferase, major domain	SUPERFAMILY	53383	PLP-dependent transferases	coord: 15..104 score: 3.7

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FD449514 ID=FD449514; Name=FD449514; organism=Cicer arietinum; type=EST; length=353bp
ACTAGCTGTGACATGAGCACTTTTAATGGAGACAGTGCTTGGAAGTGTGT
CCAAGTGTTTAACAACTTTGTCTATGTCAGACACAAGTCTCTCAGCCAAG
CTACAGCTGAAATCCTCCCTAATCACAACTCGGAGGACGGCGATGTGTTG
AGCGTCTGCCGGCATTGTATAGGCCGGAACTATCCAACCATATTTTCTTA
AATGGTCGGCTATCTCGAACACGGTGTGATGGCTACTGTCTTTCAATGTG
AAGGCTACAAGTGGCACTCCTACATCTTTGGAGAGAATGTTGAACCTTCC
TGTTTTCTCATTCCTTTCTTCAAAACTATTGTATTCTCCAAGCAATTTTC
CAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016831	carboxy-lyase activity
GO:0030170	pyridoxal phosphate binding
GO:0004351	glutamate decarboxylase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0019752	carboxylic acid metabolic process
GO:0006536	glutamate metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR002129	PyrdxlP-dep_de-COase
IPR010107	Glutamate_decarboxylase
IPR015424	PyrdxlP-dep_Trfase

Search form