FE672335

EST Overview
NameFE672335
Unique NameFE672335
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length450
Libraries
Library NameType
LIBEST_022820 Drought-stressed chickpea leave librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of FE672335 vs. SwissProt
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.175e-45
Identity = 83/150 (55.33%), Postives = 115/150 (76.67%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            +LS HR+NSF GIR+ E  +L+  L  +S  +FA++EL S+L +MTFN IMRMISGKRYYGEDCD SD++EA  FRD++ E++ + G++N  +F+ + R+LDF + EKRLK +  + D FL+GL++EHR KK+  NTMIDHLL LQ+SQP
Sbjct:  135 VLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQP 284
[Close]
BLAST of FE672335 vs. SwissProt
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 2.629e-29
Identity = 70/148 (47.30%), Postives = 95/148 (64.19%), Query Frame = -1 
Query:    7 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQES 450
            I STHRLN F  +R  E+ +L+  L R +      VELK  L ++TFN IMRM++GKRYYGE  + +D EEAK  R L+ +V +   S N  ++V I R   F  YE R+K++G   D FLQGL+D+ R +++ G TMIDHLL LQ+S
Sbjct:  143 IFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGE--ETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRL--FSSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKS 286
[Close]
BLAST of FE672335 vs. SwissProt
Match: C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 119.783 bits (299), Expect = 5.482e-27
Identity = 66/156 (42.31%), Postives = 104/156 (66.67%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGR-----ESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGD-YEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            ILS+HRL +F  IRK EI +++  L R        NDF  +EL+  L+++TFN I+RM++GKRYYG+  DV++ EEA+ F+ L+ ++    G+++  +++ I +   FG+ +EK +K +G   D  LQ L+DE RR K+ GNTM++HL+ LQ+ QP
Sbjct:  132 ILSSHRLINFQHIRKDEILRMLTRLSRYTQTSNESNDFTHIELEPLLSDLTFNNIVRMVTGKRYYGD--DVNNKEEAELFKKLVYDIAMYSGANHSADYLPILKL--FGNKFEKEVKAIGKSMDDILQRLLDECRRDKE-GNTMVNHLISLQQQQP 282
[Close]
BLAST of FE672335 vs. SwissProt
Match: C82A1_PEA (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 88.9669 bits (219), Expect = 1.036e-17
Identity = 47/133 (35.34%), Postives = 77/133 (57.89%), Query Frame = -1 
Query:   43 THRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGN 441
            +++++S YG+   +  K   T    S  D+  VELK    ++T N ++RM+ GKR +G D DV + EEAK F + I++ + + G+   G+ V   +WLD G +EK +K+   +FD  L   ++EHR KK  G+
Sbjct:  169 SNQISSQYGLL--DDTKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFG-DVDVENKEEAKRFLENIRDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGS 298
[Close]
BLAST of FE672335 vs. SwissProt
Match: C82A2_SOYBN (Cytochrome P450 82A2 OS=Glycine max GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 3.686e-15
Identity = 45/145 (31.03%), Postives = 72/145 (49.66%), Query Frame = -1 
Query:   31 LSTHRLNSFYGIRKGEIAKLVQTLGRESVND------FAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMID 447
            LS  R+   + +R  E+   +  L R+  ++      FA VELK   + + FN I+RM+ GKRY+      SD E+A      + E + +  +   G+ +   RW DFG YE  ++  G   D  +   +DEHR+K+K G  + D
Sbjct:  143 LSPSRVEQLHHVRVSEVQSSITELFRDWRSNKNVQSGFATVELKQWFSLLVFNMILRMVCGKRYFS--ASTSDDEKANRCVKAVDEFVRLAATFTVGDAIPYLRWFDFGGYENDMRETGKELDEIIGEWLDEHRQKRKMGENVQD 285
[Close]
BLAST of FE672335 vs. SwissProt
Match: C82A4_SOYBN (Cytochrome P450 82A4 OS=Glycine max GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 2.641e-13
Identity = 41/140 (29.29%), Postives = 70/140 (50.00%), Query Frame = -1 
Query:   46 ILSTHRLNSFYGIRKGEIAKLVQTL-----GRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNG 450
            ILS+ R+     +R  E+   +  L      +++ +D+A VELK    +  FN ++RM+ GKR+       +  E+A+     + E + + G    G+ +   RWLDFG YEK +K      D  +   ++EHR+K+  G
Sbjct:  145 ILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELKQWFAQPIFNMVLRMVVGKRFLSA---TATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRWLDFGGYEKAMKETAKELDVMISEWLEEHRQKRALG 281
[Close]
BLAST of FE672335 vs. SwissProt
Match: C82A3_SOYBN (Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 3.450e-13
Identity = 44/143 (30.77%), Postives = 74/143 (51.75%), Query Frame = -1 
Query:   37 LSTHRLNSFYGIRKGEIAKLVQTL------GRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTM 447
            LS  R+     IR  E+   ++ L      G ++ + +  V++K  L  +TFN ++RM+ GKRY+G    V   ++A+ F   I+E +++ G+    + V   RWLD G +EK +K      D  L   ++EHR+KK  G  +
Sbjct:  144 LSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQWLAYLTFNMVVRMVVGKRYFGV-MHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENV 285
[Close]
BLAST of FE672335 vs. SwissProt
Match: C75A1_PINTA (Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.814e-12
Identity = 36/128 (28.12%), Postives = 69/128 (53.91%), Query Frame = -1 
Query:   67 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEH 450
            +L++ R+ SF  +R+ E++ ++ ++  ES +    V +   ++    N + R+++ K++   D       + KGF DL+ EV    GS N G+F+     LD    ++ LK+  +RFD F + ++DEH
Sbjct:  145 LLTSKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILWRILARKKFSDNDLGA----DGKGFADLVVEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANARFDAFAEKMIDEH 268
[Close]
BLAST of FE672335 vs. SwissProt
Match: F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 4.217e-11
Identity = 38/131 (29.01%), Postives = 68/131 (51.91%), Query Frame = -1 
Query:   58 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRK 450
            + ST  L+ F  +R+ E+  L + L          V+L   L   T N + R++ GKR + +     D + A+ F+ ++ E++ V G  N G+F+    WLD      ++K++ +RFD FL  +++EH+ K
Sbjct:  136 LFSTKALDDFRHVRQDEVKTLTRALASAGQKP---VKLGQLLNVCTTNALARVMLGKRVFADGSGDVDPQAAE-FKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKMKKLHARFDAFLTDILEEHKGK 262
[Close]
BLAST of FE672335 vs. SwissProt
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 3.570e-10
Identity = 38/128 (29.69%), Postives = 61/128 (47.66%), Query Frame = -1 
Query:   67 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEH 450
            + S   L  F  +R+ E+  L + L R        V L   +     N + R + G+R +G D D     +A  FR ++ E++++ G  N G+FV    WLD      ++KR+  RFD FL  ++ EH
Sbjct:  136 LFSAKALEDFKHVRQEEVGTLTRELVRVGTKP---VNLGQLVNMCVVNALGREMIGRRLFGADAD----HKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKRFDAFLSSILKEH 256
[Close]
BLAST of FE672335 vs. TrEMBL
Match: B7FM85_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 2.779e-59
Identity = 115/155 (74.19%), Postives = 130/155 (83.87%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGR----ESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQE-SQP 450
            +LSTHRLNSF  IRK EI KL+Q L R     S + F +VELK+KL+EMTFNTIMRMISGKRYYGEDCDVSD EEAKGFR++IKE++S+GGSSNPGEFVGI RW DFG YEK+LKR+  RFD FLQGL+DEHRRKK+ GN MIDHLL LQE SQP
Sbjct:  134 VLSTHRLNSFLRIRKNEIMKLMQALARGSSSSSSDGFVRVELKTKLSEMTFNTIMRMISGKRYYGEDCDVSDEEEAKGFREMIKEMVSLGGSSNPGEFVGILRWFDFGGYEKKLKRIARRFDGFLQGLIDEHRRKKEKGNNMIDHLLNLQELSQP 288
[Close]
BLAST of FE672335 vs. TrEMBL
Match: Q6WNQ8_MEDTR (CYP81E8 OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 6.205e-51
Identity = 96/150 (64.00%), Postives = 119/150 (79.33%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            ILS+HRLNSF  IR+ EI +L+Q L ++S N F +VEL+   +EMTFNTIMRM+SGKRYYG DCDVSDVEEA+ FR +IKEV+S+GG++N G+F+G  RW DF   EKRLK++  R D FLQGL+DEHR  K+N NTMIDHLL  Q+SQP
Sbjct:  135 ILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQP 284
[Close]
BLAST of FE672335 vs. TrEMBL
Match: Q9ZWF2_GLYEC (Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-31 PE=2 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 2.138e-43
Identity = 81/150 (54.00%), Postives = 113/150 (75.33%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            +LS HR+NSF GIR+ E  +L+  L  +S  +FA++EL  +  +MTFN IMRMISGKRYYGEDCD+SD++EA  FRD++ E++ + G++N  +F+ + R+LDF + EKRLK +  + D FL+GL+ EHR KK+  NTMIDHLL LQ+SQP
Sbjct:  135 VLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEIELSYRFYDMTFNNIMRMISGKRYYGEDCDMSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIQEHRAKKERANTMIDHLLNLQDSQP 284
[Close]
BLAST of FE672335 vs. TrEMBL
Match: Q6WNR0_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 2.001e-41
Identity = 78/150 (52.00%), Postives = 112/150 (74.67%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            +LS HR+N+F  IR+ E  +L++ L  +S   FA+VEL  +  +MTFN IMRMISGKRYYG+DCD+S+V+EA  FRD++ E++ + G++N  +F+ + ++LDF + EKR+KR+G + D FL GL+ E R KK+  NTMIDHLL +QESQP
Sbjct:  135 VLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKRIGEKNDVFLSGLLQEQRSKKERTNTMIDHLLNMQESQP 284
[Close]
BLAST of FE672335 vs. TrEMBL
Match: Q9XFX1_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp81E2 PE=2 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 3.413e-41
Identity = 88/151 (58.28%), Postives = 108/151 (71.52%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKK-KNGNTMIDHLLKLQESQP 450
            ILSTHRLNSF  IRK E  +L+  L ++S  DF KVEL+S   E+TFNTIMRM+ GKR+YGE+ D ++ EEAK FRD++ E+   G  SN G+FV +F+  DF    K+LK+VG + D   QGLVDEHR  K KN NTMIDHLL LQESQP
Sbjct:  134 ILSTHRLNSFSEIRKDETMRLIGKLAKKSHKDFTKVELRSLFGELTFNTIMRMVCGKRFYGEESDGTNAEEAKKFRDMMNEMQEFGLGSNLGDFVPLFKLFDFSGGHKKLKKVGEKMDALFQGLVDEHRNNKDKNLNTMIDHLLSLQESQP 284
[Close]
BLAST of FE672335 vs. TrEMBL
Match: Q9XFX0_CICAR (Cytochrome P450 monooxygenase OS=Cicer arietinum GN=cyp81E3v2 PE=2 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 3.413e-41
Identity = 77/150 (51.33%), Postives = 110/150 (73.33%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            +LS HR+N+F G+R+ E  +L+  L  +S   FA+VEL  +  +MTFN IMRMISGKRYYGEDCD+SD++EA  FRD++ E++ + G++N  +F+ + ++ DF + EKR+K +  + D FL+GL+ E R KK+  NTMIDHLL LQESQP
Sbjct:  135 VLSNHRINNFSGVRRDETQRLITKLAEDSSTSFAEVELSFRFYDMTFNNIMRMISGKRYYGEDCDMSDLQEASQFRDMVTELLQLSGANNKTDFMPLLKFFDFENLEKRVKNIADKTDAFLRGLLQEQRNKKERTNTMIDHLLNLQESQP 284
[Close]
BLAST of FE672335 vs. TrEMBL
Match: Q6WNQ9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 3.413e-41
Identity = 86/155 (55.48%), Postives = 118/155 (76.13%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVND---FAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKK--KNGNTMIDHLLKLQESQP 450
            +LS +RLNSF G+R+ E  +L+Q L ++ V++   F KVEL+ +LTEMTFN +MRMISGKRYYG+D DVSDVEEAK FR++I E++S+ G++N G+F+ + R +D  + EKR KR+  R + FL+GL++EHRR     +G TMIDHLLKL ESQP
Sbjct:  133 VLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQP 287
[Close]
BLAST of FE672335 vs. TrEMBL
Match: Q9MBE4_LOTJA (Cytochrome P450 OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 7.603e-41
Identity = 79/151 (52.32%), Postives = 114/151 (75.50%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHR-RKKKNGNTMIDHLLKLQESQP 450
            +LS HR+NSF  IR+ E  +L++ L  +S  +F++VEL S+  +MTFN IMRMISGKRYYGEDCD+++++EA  FRD++ E++ + G++N  +F+ I R +DF   EKR+K + S+ D FL+GL+ EHR +K++  NTMIDHLL LQESQP
Sbjct:  135 VLSNHRINSFSPIRRDETTRLIRKLAEDSAKNFSEVELTSRFFDMTFNNIMRMISGKRYYGEDCDMTELQEASEFRDMVTELLQLSGANNKADFMPILRLVDFEGLEKRVKGISSKTDRFLRGLLQEHRDKKQRTANTMIDHLLTLQESQP 285
[Close]
BLAST of FE672335 vs. TrEMBL
Match: Q2ENF7_ASTMO (Isoflavone 3'-hydroxylase (Fragment) OS=Astragalus mongholicus PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 7.603e-41
Identity = 84/157 (53.50%), Postives = 115/157 (73.25%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVND----FAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHR---RKKKNGNTMIDHLLKLQESQP 450
            +LS HRLNSF+G+RK E  +LV+ L  + V +    F  VEL+ +LTEMTFN +MRMISGKRYYG+D DV+DV+EAK FRD+I E++S+ G++N G+F+ + R  D    EKR KR+  R D FLQGL+++H+       +G+TMIDHLLKL+E+QP
Sbjct:   93 VLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGDFLPLLRLFDLDHLEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQP 249
[Close]
BLAST of FE672335 vs. TrEMBL
Match: Q9ZRW6_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.297e-40
Identity = 76/150 (50.67%), Postives = 109/150 (72.67%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            +LS HR+N+F G+R+ E  +L+  L  +S   FA+VEL  +  +MTFN IMRMI GKRYYGEDCD+SD++EA  FRD++ E++ + G++N  +F+ + ++ DF + EKR+K +  + D FL+GL+ E R KK+  NTMIDHLL LQESQP
Sbjct:  135 VLSNHRINNFSGVRRDETQRLITKLAEDSSTSFAEVELSFRFYDMTFNNIMRMIPGKRYYGEDCDMSDLQEASQFRDMVTELLQLSGANNKTDFMPLLKFFDFENLEKRVKNIADKTDAFLRGLLQEQRNKKERTNTMIDHLLNLQESQP 284
[Close]
BLAST of FE672335 vs. TAIR peptide
Match: AT4G37330.1 (| Symbols: CYP81D4 | cytochrome P450, family 81, subfamily D, polypeptide 4 | chr4:17562547-17564569 REVERSE LENGTH=492)

HSP 1 Score: 153.68 bits (387), Expect = 4.035e-38
Identity = 77/150 (51.33%), Postives = 107/150 (71.33%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            I STHRLNSF  IRK EI +L+  L R+S++ F +VE+K+ LT +  NT +RM++GKRY+GED D     +AK  ++L+ E ++  G+ NP +++ I RW+    YEKR+K +G+RFDTFLQ LVDE R +K+ G TMIDHLL LQ+ QP
Sbjct:  137 IFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTTIRMLAGKRYFGEDND-----DAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRIKNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQP 279
[Close]
BLAST of FE672335 vs. TAIR peptide
Match: AT4G37370.1 (| Symbols: CYP81D8 | cytochrome P450, family 81, subfamily D, polypeptide 8 | chr4:17569971-17571678 REVERSE LENGTH=497)

HSP 1 Score: 152.14 bits (383), Expect = 1.174e-37
Identity = 79/150 (52.67%), Postives = 103/150 (68.67%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            I S HRLNSF  IRK EI +LV  L R    +F KV++KS L+++TFN I+RM++GKRYYG+   V D  EAK  R LI +V++  G+ N  +++ + R +   DYE R+K++  R D FLQGLVDE R  K+ GNTMIDHLL LQESQP
Sbjct:  136 IFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGD--GVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQP 281
[Close]
BLAST of FE672335 vs. TAIR peptide
Match: AT4G37340.1 (| Symbols: CYP81D3 | cytochrome P450, family 81, subfamily D, polypeptide 3 | chr4:17564953-17566706 REVERSE LENGTH=500)

HSP 1 Score: 138.658 bits (348), Expect = 1.343e-33
Identity = 75/151 (49.67%), Postives = 100/151 (66.23%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRR-KKKNGNTMIDHLLKLQESQP 450
            I S HRLNSF  IR+ EI +L+  L R S  +F KVE+KS  + +TFN I+RM++GK YYG+  +  D  EAK  R+LI E +   G+ N  +++ I  W+     EKR+K++ SR D FLQGLVDE R  K+K  NTM+DHLL LQE+QP
Sbjct:  136 IFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRMLAGKCYYGDGAE--DDPEAKRVRELIAEGMGCFGAGNTADYLPILTWIT--GSEKRIKKIASRLDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQP 282
[Close]
BLAST of FE672335 vs. TAIR peptide
Match: AT2G23220.1 (| Symbols: CYP81D6 | cytochrome P450, family 81, subfamily D, polypeptide 6 | chr2:9884550-9886752 FORWARD LENGTH=515)

HSP 1 Score: 137.887 bits (346), Expect = 2.291e-33
Identity = 69/150 (46.00%), Postives = 100/150 (66.67%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            I ++H+LN F  +RK EI +L+  L + S + FAKVE++  L E+T N + RM++GKR+YGE  +  +V  A+  R L+ E+++  G+ N  +++ I RW  F ++EKR+K +  R D FLQ LVDE R  K+ G TMIDHLL LQESQP
Sbjct:  152 IFASHKLNGFLSVRKDEIRQLLLRLSKNSRHGFAKVEMRHLLFELTINNVFRMVAGKRFYGEGTEQDEV--AQQVRHLMDEIVTSAGAGNAADYLPIMRW--FTNFEKRVKNLAIRIDKFLQSLVDEKRADKEKGTTMIDHLLSLQESQP 297
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BLAST of FE672335 vs. TAIR peptide
Match: AT4G37360.1 (| Symbols: CYP81D2 | cytochrome P450, family 81, subfamily D, polypeptide 2 | chr4:17567124-17568858 REVERSE LENGTH=499)

HSP 1 Score: 135.576 bits (340), Expect = 1.137e-32
Identity = 74/151 (49.01%), Postives = 98/151 (64.90%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRR-KKKNGNTMIDHLLKLQESQP 450
            I S HRLNSF  IR+ EI +L+  L R S   F KVE+KS  +++TFN I+RM++GK YYG+  +  D  EAK  R LI E +S  G  N  +++ I  W+ +   E R+K++  R D FLQGLVDE R  K+K  NTM+DHLL LQE+QP
Sbjct:  136 IFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIRMLAGKCYYGDGKE--DDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRIKKLAGRLDEFLQGLVDEKREGKEKKENTMVDHLLCLQETQP 282
[Close]
BLAST of FE672335 vs. TAIR peptide
Match: AT2G23190.1 (| Symbols: CYP81D7 | cytochrome P450, family 81, subfamily D, polypeptide 7 | chr2:9877058-9879007 FORWARD LENGTH=543)

HSP 1 Score: 134.806 bits (338), Expect = 1.940e-32
Identity = 68/150 (45.33%), Postives = 97/150 (64.67%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            I S+H+LN F  +RK EI  L+  L + S + FAKVE++    ++T N I+RM++GKR+YGE  +  +V  A+    LI E++   G  N  +++ I RW+   D+EK +K + SR D FLQ LVDE R  K+ GNTM+DHLL LQE+QP
Sbjct:  181 IFSSHKLNGFLSVRKDEIRHLLLRLSKNSQHGFAKVEMRQLFYDLTINNILRMVAGKRFYGEGTEQDEV--ARRVTQLIDEIVYRAGVGNAADYIPILRWIT--DFEKGVKELASRVDEFLQSLVDERRVHKQKGNTMMDHLLSLQETQP 326
[Close]
BLAST of FE672335 vs. TAIR peptide
Match: AT4G37320.1 (| Symbols: CYP81D5 | cytochrome P450, family 81, subfamily D, polypeptide 5 | chr4:17559742-17561690 REVERSE LENGTH=495)

HSP 1 Score: 131.339 bits (329), Expect = 2.144e-31
Identity = 67/150 (44.67%), Postives = 101/150 (67.33%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            I S+HR+++F  IRK EI +L+  L R+S++ F +VELKS LT + FN I+ M++GKRYYG   +  D +EAK  R+LI E+++  GS N  +++    W+   ++E + K +G+R D  LQ LVDE R +K+ G T+IDHLL  QE++P
Sbjct:  137 IFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNIIMMVAGKRYYGTGTE--DNDEAKLVRELIAEIMAGAGSGNLADYLPSINWVT--NFENQTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEP 282
[Close]
BLAST of FE672335 vs. TAIR peptide
Match: AT1G66540.1 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24824837-24826502 FORWARD LENGTH=386)

HSP 1 Score: 131.339 bits (329), Expect = 2.144e-31
Identity = 73/153 (47.71%), Postives = 100/153 (65.36%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRE--SVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHR-RKKKNGNTMIDHLLKLQESQP 450
            I S HRLNSFY IR+ EI +L+  L R   +  +FAKVE+ S L+ + FN I+RM++GK YYG+  +  D  EAK  R LI E +S  G+ +  + + + RW+   D+E+R+K++ +R D F Q LVDE R  K+K  NTMIDHLL LQ SQP
Sbjct:   21 IFSNHRLNSFYTIRRDEIRRLIARLSRSPNASLEFAKVEMNSMLSNLAFNNIIRMVTGKCYYGDGAE--DDPEAKRVRQLIAEAMSCFGAGHAADHLPMLRWIT--DFERRVKKIAARLDEFFQRLVDEKRVAKEKKENTMIDHLLSLQVSQP 169
[Close]
BLAST of FE672335 vs. TAIR peptide
Match: AT3G28740.1 (| Symbols: CYP81D1 | Cytochrome P450 superfamily protein | chr3:10788764-10790552 REVERSE LENGTH=509)

HSP 1 Score: 129.413 bits (324), Expect = 8.149e-31
Identity = 70/150 (46.67%), Postives = 98/150 (65.33%), Query Frame = -1 
Query:    1 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQESQP 450
            I S+ RLNSF  IR+ EI +L+  L + S + F KVE+K     +T N I+RM++GKR+YG+  +  +  EAK  R LI EV+  GG+ N  ++  I R++   +YEK +K++  R D FLQ LV+E R +K  GNTMIDHLL LQE+QP
Sbjct:  145 IFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIRMVAGKRFYGDGTE--NDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVT--NYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQP 290
[Close]
BLAST of FE672335 vs. TAIR peptide
Match: AT5G36220.1 (| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1 | chr5:14253827-14256015 REVERSE LENGTH=502)

HSP 1 Score: 127.487 bits (319), Expect = 3.097e-30
Identity = 70/148 (47.30%), Postives = 95/148 (64.19%), Query Frame = -1 
Query:    7 ILSTHRLNSFYGIRKGEIAKLVQTLGRESVNDFAKVELKSKLTEMTFNTIMRMISGKRYYGEDCDVSDVEEAKGFRDLIKEVISVGGSSNPGEFVGIFRWLDFGDYEKRLKRVGSRFDTFLQGLVDEHRRKKKNGNTMIDHLLKLQES 450
            I STHRLN F  +R  E+ +L+  L R +      VELK  L ++TFN IMRM++GKRYYGE  + +D EEAK  R L+ +V +   S N  ++V I R   F  YE R+K++G   D FLQGL+D+ R +++ G TMIDHLL LQ+S
Sbjct:  143 IFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGE--ETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRL--FSSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKS 286
The following BLAST results are available for this feature:
BLAST of FE672335 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
C81E1_GLYEC1.175e-4555.33Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata ... [more]
C81D1_ARATH2.629e-2947.30Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CY... [more]
C81F1_ARATH5.482e-2742.31Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CY... [more]
C82A1_PEA1.036e-1735.34Cytochrome P450 82A1 (Fragment) OS=Pisum sativum G... [more]
C82A2_SOYBN3.686e-1531.03Cytochrome P450 82A2 OS=Glycine max GN=CYP82A2 PE=... [more]
C82A4_SOYBN2.641e-1329.29Cytochrome P450 82A4 OS=Glycine max GN=CYP82A4 PE=... [more]
C82A3_SOYBN3.450e-1330.77Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=... [more]
C75A1_PINTA3.814e-1228.13Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 P... [more]
F3PH_PETHY4.217e-1129.01Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=C... [more]
F3PH_ARATH3.570e-1029.69Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana... [more]
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BLAST of FE672335 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B7FM85_MEDTR2.779e-5974.19Putative uncharacterized protein OS=Medicago trunc... [more]
Q6WNQ8_MEDTR6.205e-5164.00CYP81E8 OS=Medicago truncatula PE=2 SV=1[more]
Q9ZWF2_GLYEC2.138e-4354.00Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-... [more]
Q6WNR0_MEDTR2.001e-4152.00Isoflavone 2'-hydroxylase OS=Medicago truncatula P... [more]
Q9XFX1_CICAR3.413e-4158.28Cytochrome P450 OS=Cicer arietinum GN=cyp81E2 PE=2... [more]
Q9XFX0_CICAR3.413e-4151.33Cytochrome P450 monooxygenase OS=Cicer arietinum G... [more]
Q6WNQ9_MEDTR3.413e-4155.48Isoflavone 3'-hydroxylase (Fragment) OS=Medicago t... [more]
Q9MBE4_LOTJA7.603e-4152.32Cytochrome P450 OS=Lotus japonicus PE=2 SV=1[more]
Q2ENF7_ASTMO7.603e-4153.50Isoflavone 3'-hydroxylase (Fragment) OS=Astragalus... [more]
Q9ZRW6_CICAR1.297e-4050.67Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2... [more]
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BLAST of FE672335 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10
Match NameE-valueIdentityDescription
AT4G37330.14.035e-3851.33| Symbols: CYP81D4 | cytochrome P450, family 81, s... [more]
AT4G37370.11.174e-3752.67| Symbols: CYP81D8 | cytochrome P450, family 81, s... [more]
AT4G37340.11.343e-3349.67| Symbols: CYP81D3 | cytochrome P450, family 81, s... [more]
AT2G23220.12.291e-3346.00| Symbols: CYP81D6 | cytochrome P450, family 81, s... [more]
AT4G37360.11.137e-3249.01| Symbols: CYP81D2 | cytochrome P450, family 81, s... [more]
AT2G23190.11.940e-3245.33| Symbols: CYP81D7 | cytochrome P450, family 81, s... [more]
AT4G37320.12.144e-3144.67| Symbols: CYP81D5 | cytochrome P450, family 81, s... [more]
AT1G66540.12.144e-3147.71| Symbols: | Cytochrome P450 superfamily protein ... [more]
AT3G28740.18.149e-3146.67| Symbols: CYP81D1 | Cytochrome P450 superfamily p... [more]
AT5G36220.13.097e-3047.30| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450GENE3D1.10.630.10no descriptioncoord: 2..149
score: 1.2
IPR001128Cytochrome P450PANTHERPTHR19383CYTOCHROME P450coord: 1..150
score: 1.8
IPR001128Cytochrome P450PFAMPF00067p450coord: 6..146
score: 9.4
IPR001128Cytochrome P450SUPERFAMILY48264Cytochrome P450coord: 1..149
score: 5.6
NoneNo IPR availablePANTHERPTHR19383:SF42CYTOCHROME P450 SUBFAMILY 81Dcoord: 1..150
score: 1.8

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FE672335 ID=FE672335; Name=FE672335; organism=Cicer arietinum; type=EST; length=450bp
TGGCTGTGATTCTTGCAACTTCAATAAATGATCAATCATGGTGTTCCCGT
TTTTCTTCTTTCTACGATGTTCATCAACAAGTCCCTGCAAAAACGTATCA
AATCTACTACCAACCCTCTTCAACCTCTTCTCATAATCACCAAAATCTAA
CCACCTAAAAATCCCAACAAATTCACCAGGATTACTCGATCCTCCCACAG
ATATAACCTCTTTAATCAAATCCCTAAACCCCTTCGCTTCTTCCACATCA
CTCACATCACAATCCTCTCCATAATACCTCTTTCCCGATATCATTCTCAT
TATCGTGTTAAATGTCATCTCCGTTAACTTCGATTTCAGTTCCACTTTCG
CGAAATCGTTAACAGATTCACGACCTAGGGTTTGCACGAGTTTCGCGATT
TCGCCTTTTCTGATTCCGTAAAAGGAGTTTAAACGGTGTGTTGAGAGAAT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004497monooxygenase activity
GO:0005506iron ion binding
GO:0009055electron transfer activity
GO:0020037heme binding
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450