GR914969

EST Overview


Name	GR914969
Unique Name	GR914969
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	222

Libraries


Library Name	Type
LIBEST_024968 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. JG-62) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR914969 vs. SwissProt
Match: KCAB_SCHPO (Putative voltage-gated potassium channel subunit beta OS=Schizosaccharomyces pombe GN=SPCC965.06 PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 8.510e-15
Identity = 35/56 (62.50%), Postives = 46/56 (82.14%), Query Frame = -2

Query:   51 KEPNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVIRN 218
            K PNT G+SRKH++EGL +SLKRL  PYVDV+ AHRPD + PM+E+V  FTQ+I++
Sbjct:  103 KLPNTTGLSRKHIIEGLNASLKRLGLPYVDVIMAHRPDPSVPMEEVVRAFTQLIQD 158

[Close]

BLAST of GR914969 vs. SwissProt
Match: KCAB1_RAT (Voltage-gated potassium channel subunit beta-1 OS=Rattus norvegicus GN=Kcnab1 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.806e-12
Identity = 32/48 (66.67%), Postives = 39/48 (81.25%), Query Frame = -2

Query:   57 GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVI 200
            G+SRKH++EGLK SL+RL+  YVDVVFA+RPD  TPM+EIV   T VI
Sbjct:  164 GLSRKHIIEGLKGSLQRLQLEYVDVVFANRPDSNTPMEEIVRAMTHVI 211

[Close]

BLAST of GR914969 vs. SwissProt
Match: KCAB1_RABIT (Voltage-gated potassium channel subunit beta-1 OS=Oryctolagus cuniculus GN=KCNAB1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.806e-12
Identity = 32/48 (66.67%), Postives = 39/48 (81.25%), Query Frame = -2

Query:   57 GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVI 200
            G+SRKH++EGLK SL+RL+  YVDVVFA+RPD  TPM+EIV   T VI
Sbjct:  182 GLSRKHIIEGLKGSLQRLQLEYVDVVFANRPDSNTPMEEIVRAMTHVI 229

[Close]

BLAST of GR914969 vs. SwissProt
Match: KCAB1_MUSPU (Voltage-gated potassium channel subunit beta-1 OS=Mustela putorius GN=KCNAB1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.806e-12
Identity = 32/48 (66.67%), Postives = 39/48 (81.25%), Query Frame = -2

Query:   57 GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVI 200
            G+SRKH++EGLK SL+RL+  YVDVVFA+RPD  TPM+EIV   T VI
Sbjct:  171 GLSRKHIIEGLKGSLQRLQLEYVDVVFANRPDSNTPMEEIVRAMTHVI 218

[Close]

BLAST of GR914969 vs. SwissProt
Match: KCAB1_MOUSE (Voltage-gated potassium channel subunit beta-1 OS=Mus musculus GN=Kcnab1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.806e-12
Identity = 32/48 (66.67%), Postives = 39/48 (81.25%), Query Frame = -2

Query:   57 GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVI 200
            G+SRKH++EGLK SL+RL+  YVDVVFA+RPD  TPM+EIV   T VI
Sbjct:  164 GLSRKHIIEGLKGSLQRLQLEYVDVVFANRPDSNTPMEEIVRAMTHVI 211

[Close]

BLAST of GR914969 vs. SwissProt
Match: KCAB1_HUMAN (Voltage-gated potassium channel subunit beta-1 OS=Homo sapiens GN=KCNAB1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.806e-12
Identity = 32/48 (66.67%), Postives = 39/48 (81.25%), Query Frame = -2

Query:   57 GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVI 200
            G+SRKH++EGLK SL+RL+  YVDVVFA+RPD  TPM+EIV   T VI
Sbjct:  182 GLSRKHIIEGLKGSLQRLQLEYVDVVFANRPDSNTPMEEIVRAMTHVI 229

[Close]

BLAST of GR914969 vs. SwissProt
Match: KCAB1_BOVIN (Voltage-gated potassium channel subunit beta-1 OS=Bos taurus GN=KCNAB1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.806e-12
Identity = 32/48 (66.67%), Postives = 39/48 (81.25%), Query Frame = -2

Query:   57 GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVI 200
            G+SRKH++EGLK SL+RL+  YVDVVFA+RPD  TPM+EIV   T VI
Sbjct:  164 GLSRKHIIEGLKGSLQRLQLEYVDVVFANRPDSNTPMEEIVRAMTHVI 211

[Close]

BLAST of GR914969 vs. SwissProt
Match: KCAB_ORYSJ (Probable voltage-gated potassium channel subunit beta OS=Oryza sativa subsp. japonica GN=KOB1 PE=1 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 1.502e-11
Identity = 31/52 (59.62%), Postives = 38/52 (73.08%), Query Frame = -2

Query:   57 PNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVI 212
            PN  G+SRKH+VEGL+ SLKRL+  YVDVV+ HRPD  TP++E V     VI
Sbjct:   90 PNDKGLSRKHIVEGLRGSLKRLDMDYVDVVYCHRPDATTPVEETVRAMNWVI 141

[Close]

BLAST of GR914969 vs. SwissProt
Match: KCAB1_CHICK (Voltage-gated potassium channel subunit beta-1 OS=Gallus gallus GN=KCNAB1 PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.502e-11
Identity = 31/48 (64.58%), Postives = 40/48 (83.33%), Query Frame = -2

Query:   57 GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVI 200
            G+SRKH++EGL++SL+RL+  YVDVVFA+RPD  TPM+EIV   T VI
Sbjct:  164 GLSRKHIIEGLRASLQRLQLEYVDVVFANRPDNNTPMEEIVRAMTHVI 211

[Close]

BLAST of GR914969 vs. SwissProt
Match: KCAB2_XENLA (Voltage-gated potassium channel subunit beta-2 OS=Xenopus laevis GN=kcnab2 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 3.346e-11
Identity = 31/48 (64.58%), Postives = 39/48 (81.25%), Query Frame = -2

Query:   57 GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVI 200
            G+SRKH++EGLK+SL+RL+  YVDVVFA+RPD  TPM+E V   T VI
Sbjct:  130 GLSRKHIIEGLKASLERLQLDYVDVVFANRPDPNTPMEETVRAMTHVI 177

[Close]

BLAST of GR914969 vs. TrEMBL
Match: C7ZAY6_NECH7 (Predicted protein OS=Nectria haematococca (strain 77-13-4 / FGSC 9596 / MPVI) GN=NECHADRAFT_70743 PE=4 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.316e-24
Identity = 54/61 (88.52%), Postives = 60/61 (98.36%), Query Frame = -2

Query:   39 RKEPNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVIRNLNLA 221
            RKEPNT G+SRKH+VEGLKSSL+RL+QPYVD+VFAHRPDYATP+KEIVEGFTQVIRNLNLA
Sbjct:  101 RKEPNTRGLSRKHIVEGLKSSLERLQQPYVDIVFAHRPDYATPLKEIVEGFTQVIRNLNLA 161

[Close]

BLAST of GR914969 vs. TrEMBL
Match: C9SVP2_VERA1 (Voltage-gated potassium channel subunit beta-3 OS=Verticillium albo-atrum (strain VaMs.102) GN=VDBG_08967 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.830e-24
Identity = 55/61 (90.16%), Postives = 59/61 (96.72%), Query Frame = -2

Query:   39 RKEPNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVIRNLNLA 221
            RKEPNT G+S KH+VEGLKSSL+RL+QPYVDVVFAHRPDYATPMKEIVEGFTQVIRNLNLA
Sbjct:  101 RKEPNTRGLSWKHIVEGLKSSLQRLDQPYVDVVFAHRPDYATPMKEIVEGFTQVIRNLNLA 161

[Close]

BLAST of GR914969 vs. TrEMBL
Match: D1Z801_SORMA (Whole genome shotgun sequence assembly, contig_3154 OS=Sordaria macrospora GN=SMAC_00541 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.043e-21
Identity = 51/61 (83.61%), Postives = 56/61 (91.80%), Query Frame = -2

Query:   39 RKEPNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVIRNLNLA 221
            RKEPNT G+SRKH+VEGLK+S+KRL+ PYVDVVFAHRPD ATPM EIVE FTQVIRNLNLA
Sbjct:  105 RKEPNTRGLSRKHIVEGLKASIKRLQTPYVDVVFAHRPDAATPMLEIVEAFTQVIRNLNLA 165

[Close]

BLAST of GR914969 vs. TrEMBL
Match: B2A9P4_PODAN (Predicted CDS Pa_1_1240 OS=Podospora anserina PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 3.963e-21
Identity = 51/61 (83.61%), Postives = 54/61 (88.52%), Query Frame = -2

Query:   39 RKEPNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVIRNLNLA 221
            RKEPNT G+SRKH+VEGLKSSL RL+QPYVDVVFAHRPD   PM EIVE FTQVIRNLNLA
Sbjct:  101 RKEPNTRGLSRKHIVEGLKSSLARLQQPYVDVVFAHRPDPTVPMLEIVEAFTQVIRNLNLA 161

[Close]

BLAST of GR914969 vs. TrEMBL
Match: Q7SHL7_NEUCR (Voltage-gated potassium channel beta-2 subunit OS=Neurospora crassa GN=NCU02887 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.176e-21
Identity = 50/61 (81.97%), Postives = 55/61 (90.16%), Query Frame = -2

Query:   39 RKEPNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVIRNLNLA 221
            RKEPNT G+SRKH+VEGLK+S+ RL+ PYVDVVFAHRPD ATPM EIVE FTQVIRNLNLA
Sbjct:  105 RKEPNTRGLSRKHIVEGLKASVNRLQTPYVDVVFAHRPDAATPMLEIVEAFTQVIRNLNLA 165

[Close]

BLAST of GR914969 vs. TrEMBL
Match: Q2HCP3_CHAGB (Putative uncharacterized protein OS=Chaetomium globosum GN=CHGG_02011 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.175e-19
Identity = 48/61 (78.69%), Postives = 52/61 (85.25%), Query Frame = -2

Query:   39 RKEPNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVIRNLNLA 221
            R EPNT G+SRKH++EGLKSSL RL+ PYVDVVFAHR D ATPM EIVE FTQ IRNLNLA
Sbjct:  103 RAEPNTGGLSRKHIIEGLKSSLARLQTPYVDVVFAHRADAATPMLEIVEAFTQAIRNLNLA 163

[Close]

BLAST of GR914969 vs. TrEMBL
Match: Q0UV21_PHANO (Putative uncharacterized protein OS=Phaeosphaeria nodorum GN=SNOG_04393 PE=4 SV=2)

HSP 1 Score: 86.6557 bits (213), Expect = 8.551e-16
Identity = 42/61 (68.85%), Postives = 51/61 (83.61%), Query Frame = -2

Query:   39 RKEPNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVIRNLNLA 221
            RKEPNT G+S+KH++EGLKSSL RL+ PYVD+VFAHR D   PM+EIVE FTQ I ++NLA
Sbjct:   99 RKEPNTRGLSKKHIIEGLKSSLARLQTPYVDIVFAHRHDPDVPMREIVEAFTQTI-HMNLA 158

[Close]

BLAST of GR914969 vs. TrEMBL
Match: B2WDD1_PYRTR (Voltage-gated potassium channel subunit beta-1 channel subunit beta-1 OS=Pyrenophora tritici-repentis (strain Pt-1C-BFP) GN=PTRG_07990 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.543e-15
Identity = 44/61 (72.13%), Postives = 49/61 (80.33%), Query Frame = -2

Query:   39 RKEPNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVIRNLNLA 221
            RKEPNT G+S+KH+VEGLKSSL RL+  YVDVVFAHR D    MKEIVE FTQ I N+NLA
Sbjct:  102 RKEPNTRGLSKKHIVEGLKSSLARLDHSYVDVVFAHRFDPEVGMKEIVEAFTQTI-NMNLA 161

[Close]

BLAST of GR914969 vs. TrEMBL
Match: Q6CI80_YARLI (YALI0A00847p OS=Yarrowia lipolytica GN=YALI0A00847g PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.736e-12
Identity = 35/56 (62.50%), Postives = 43/56 (76.79%), Query Frame = -2

Query:   51 KEPNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVIRN 218
            K  N  G+SRKHV+EG+  SLKRL+  YVDVVFAHRPD  TP++E+V  FTQVI +
Sbjct:   96 KTVNALGLSRKHVLEGIDESLKRLQLDYVDVVFAHRPDRYTPIEEVVRAFTQVIND 151

[Close]

BLAST of GR914969 vs. TrEMBL
Match: B0DLU1_LACBS (Predicted protein OS=Laccaria bicolor (strain S238N-H82) GN=LACBIDRAFT_252630 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.736e-12
Identity = 35/55 (63.64%), Postives = 41/55 (74.55%), Query Frame = -2

Query:   57 RKEPNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVI 221
            RK PN  G+SRKH+VEG K SL+RL   YVDV+FAHRPD+  PM+EIV  F  VI
Sbjct:  102 RKGPNDSGLSRKHIVEGTKESLERLGLDYVDVIFAHRPDHTVPMEEIVRAFNFVI 156

[Close]

BLAST of GR914969 vs. TAIR peptide
Match: AT1G04690.1 (| Symbols: KAB1, KV-BETA1 | potassium channel beta subunit 1 | chr1:1313662-1315420 FORWARD LENGTH=328)

HSP 1 Score: 67.3958 bits (163), Expect = 2.066e-12
Identity = 30/52 (57.69%), Postives = 39/52 (75.00%), Query Frame = -2

Query:   57 PNT*GISRKHVVEGLKSSLKRLEQPYVDVVFAHRPDYATPMKEIVEGFTQVI 212
            PN  G+SRKH+VEG K+SLKRL+  YVDV++ HRPD +TP++E V     VI
Sbjct:   90 PNDKGLSRKHIVEGTKASLKRLDMDYVDVLYCHRPDASTPIEETVRAMNYVI 141

The following BLAST results are available for this feature:

BLAST of GR914969 vs. SwissProt
BLAST of GR914969 vs. TrEMBL
BLAST of GR914969 vs. TAIR peptide

BLAST of GR914969 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
KCAB_SCHPO	8.510e-15	62.50	Putative voltage-gated potassium channel subunit b...	[more]
KCAB1_RAT	8.806e-12	66.67	Voltage-gated potassium channel subunit beta-1 OS=...	[more]
KCAB1_RABIT	8.806e-12	66.67	Voltage-gated potassium channel subunit beta-1 OS=...	[more]
KCAB1_MUSPU	8.806e-12	66.67	Voltage-gated potassium channel subunit beta-1 OS=...	[more]
KCAB1_MOUSE	8.806e-12	66.67	Voltage-gated potassium channel subunit beta-1 OS=...	[more]
KCAB1_HUMAN	8.806e-12	66.67	Voltage-gated potassium channel subunit beta-1 OS=...	[more]
KCAB1_BOVIN	8.806e-12	66.67	Voltage-gated potassium channel subunit beta-1 OS=...	[more]
KCAB_ORYSJ	1.502e-11	59.62	Probable voltage-gated potassium channel subunit b...	[more]
KCAB1_CHICK	1.502e-11	64.58	Voltage-gated potassium channel subunit beta-1 OS=...	[more]
KCAB2_XENLA	3.346e-11	64.58	Voltage-gated potassium channel subunit beta-2 OS=...	[more]

BLAST of GR914969 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C7ZAY6_NECH7	1.316e-24	88.52	Predicted protein OS=Nectria haematococca (strain ...	[more]
C9SVP2_VERA1	3.830e-24	90.16	Voltage-gated potassium channel subunit beta-3 OS=...	[more]
D1Z801_SORMA	1.043e-21	83.61	Whole genome shotgun sequence assembly, contig_315...	[more]
B2A9P4_PODAN	3.963e-21	83.61	Predicted CDS Pa_1_1240 OS=Podospora anserina PE=4...	[more]
Q7SHL7_NEUCR	5.176e-21	81.97	Voltage-gated potassium channel beta-2 subunit OS=...	[more]
Q2HCP3_CHAGB	2.175e-19	78.69	Putative uncharacterized protein OS=Chaetomium glo...	[more]
Q0UV21_PHANO	8.551e-16	68.85	Putative uncharacterized protein OS=Phaeosphaeria ...	[more]
B2WDD1_PYRTR	5.543e-15	72.13	Voltage-gated potassium channel subunit beta-1 cha...	[more]
Q6CI80_YARLI	5.736e-12	62.50	YALI0A00847p OS=Yarrowia lipolytica GN=YALI0A00847...	[more]
B0DLU1_LACBS	5.736e-12	63.64	Predicted protein OS=Laccaria bicolor (strain S238...	[more]

BLAST of GR914969 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G04690.1	2.066e-12	57.69	\| Symbols: KAB1, KV-BETA1 \| potassium channel beta...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001395	Aldo/keto reductase	PANTHER	PTHR11732	ALDO/KETO REDUCTASE	coord: 1..50 score: 4.9
IPR005399	Potassium channel, voltage-dependent, beta subunit, KCNAB-related	PANTHER	PTHR11732:SF14	POTASSIUM CHANNEL BETA	coord: 1..50 score: 4.9
IPR023210	NADP-dependent oxidoreductase domain	GENE3D	3.20.20.100	no description	coord: 2..49 score: 1.2
IPR023210	NADP-dependent oxidoreductase domain	PFAM	PF00248	Aldo_ket_red	coord: 2..49 score: 2.3
IPR023210	NADP-dependent oxidoreductase domain	SUPERFAMILY	51430	NAD(P)-linked oxidoreductase	coord: 1..49 score: 6.5

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR914969 ID=GR914969; Name=GR914969; organism=Cicer arietinum; type=EST; length=222bp
GATTTCGAGCGGCCGCCCGGGCAGGTACCCCTTTACTAAGCAAGGTTCAG
GTTGCGAATGACCTGAGTAAATCCCTCAACAATCTCCTTCATGGGAGTAG
CGTAGTCGGGACGGTGAGCAAAGACAACATCGACGTAAGGCTGCTCAAGA
CGCTTGAGAGAGCTCTTGAGACCCTCGACGACGTGCTTGCGGCTGATACC
TCAAGTGTTGGGCTCCTTGCGG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process
GO:0006813	potassium ion transport
GO:0055085	transmembrane transport

Vocabulary: **INTERPRO**
Term	Definition
IPR001395	Aldo/ket_red/Kv-b
IPR005399	K_chnl_volt-dep_bsu_KCNAB-rel
IPR023210	NADP_OxRdtase_dom

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