GR395758

EST Overview


Name	GR395758
Unique Name	GR395758
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	689

Libraries


Library Name	Type
LIBEST_024781 ICC4958 dehydration stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR395758 vs. SwissProt
Match: 7OMT9_MEDSA (Isoflavone-7-O-methyltransferase 9 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 6.735e-29
Identity = 62/80 (77.50%), Postives = 70/80 (87.50%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L+  ++ +   GK+GKV +I MVINEKKDENQ+TQIKLLMDVNMACLNGKERNE+EWKKLFIEA FQ YKISPLTGFLSL
Sbjct:  268 LKKCKEAVTNDGKRGKVTIIDMVINEKKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSL 347

HSP 2 Score: 60.4622 bits (145), Expect = 1.011e-8
Identity = 24/34 (70.59%), Postives = 32/34 (94.12%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKKRK 108
            + +K+ILHNWTDKDC+RIL+KCKEAVT++GK+ K
Sbjct:  250 VLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGK 283

[Close]

BLAST of GR395758 vs. SwissProt
Match: 7OMT6_MEDSA (Isoflavone-7-O-methyltransferase 6 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 2.559e-28
Identity = 61/80 (76.25%), Postives = 70/80 (87.50%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L+  ++ +   GK+GKV +I MVI+EKKDENQ+TQIKLLMDVNMACLNGKERNE+EWKKLFIEA FQ YKISPLTGFLSL
Sbjct:  268 LKKCKEAVTNDGKRGKVTIIDMVIDEKKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSL 347

HSP 2 Score: 60.4622 bits (145), Expect = 1.011e-8
Identity = 24/34 (70.59%), Postives = 32/34 (94.12%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKKRK 108
            + +K+ILHNWTDKDC+RIL+KCKEAVT++GK+ K
Sbjct:  250 VLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGK 283

[Close]

BLAST of GR395758 vs. SwissProt
Match: 7OMT8_MEDSA (Isoflavone-7-O-methyltransferase 8 OS=Medicago sativa PE=1 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 7.446e-28
Identity = 60/80 (75.00%), Postives = 70/80 (87.50%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L+  ++ +   GK+GKV +I MVI++KKDENQ+TQIKLLMDVNMACLNGKERNE+EWKKLFIEA FQ YKISPLTGFLSL
Sbjct:  268 LKKCKEAVTNDGKRGKVTIIDMVIDKKKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSL 347

HSP 2 Score: 60.4622 bits (145), Expect = 1.011e-8
Identity = 24/34 (70.59%), Postives = 32/34 (94.12%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKKRK 108
            + +K+ILHNWTDKDC+RIL+KCKEAVT++GK+ K
Sbjct:  250 VLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGK 283

[Close]

BLAST of GR395758 vs. SwissProt
Match: ZRP4_MAIZE (O-methyltransferase ZRP4 OS=Zea mays GN=ZRP4 PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 4.190e-14
Identity = 29/80 (36.25%), Postives = 49/80 (61.25%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L+N +K +      GKVI+I MV+     + +  +++ + DV +  +NG ER+E+EW K+F EA + DY+I P+ G  S+
Sbjct:  280 LKNCKKAIPPREAGGKVIIINMVVGAGPSDMKHKEMQAIFDVYIMFINGMERDEQEWSKIFSEAGYSDYRIIPVLGVRSI 359

HSP 2 Score: 35.4242 bits (80), Expect = 4.190e-14
Identity = 11/26 (42.31%), Postives = 20/26 (76.92%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAV 84
            + +K +LH+W   DC++IL+ CK+A+
Sbjct:  262 VLLKSVLHDWDHDDCVKILKNCKKAI 287

[Close]

BLAST of GR395758 vs. SwissProt
Match: 6OMT_COPJA ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 5.450e-14
Identity = 27/69 (39.13%), Postives = 47/69 (68.12%), Query Frame = 2

Query:   98 KKGKVIVIGMVINEKKDENQITQIKLLMDVNMAC-LNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            K GKVI++ +V+N +  E+  T+++L +D++M     GKER E+EWKKL  +A ++ +KI+ +T   S+
Sbjct:  274 KGGKVIIVDIVLNVQS-EHPYTKMRLTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSV 341

HSP 2 Score: 45.8246 bits (107), Expect = 5.450e-14
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKK 102
            + MK ILH+W DK+CI IL++CKEAV   G K
Sbjct:  246 IMMKCILHDWDDKECIEILKRCKEAVPVKGGK 277

[Close]

BLAST of GR395758 vs. SwissProt
Match: 4OMT_COPJA (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 41.2022 bits (95), Expect = 6.809e-9
Identity = 20/67 (29.85%), Postives = 41/67 (61.19%), Query Frame = 2

Query:  104 GKVIVIGMVINEKKDENQITQIKLLMDVNMAC-LNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            GKVI++ + ++E+ D ++++  +L++D++M     GKER ++ W+K+   A F   KI  +    S+
Sbjct:  280 GKVIIVDVALDEESD-HELSSTRLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSV 345

HSP 2 Score: 39.6614 bits (91), Expect = 6.809e-9
Identity = 15/32 (46.88%), Postives = 24/32 (75.00%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKK 102
            + +K ILH+W D+D I+IL++C+ AV  +G K
Sbjct:  250 IILKLILHDWNDEDSIKILKQCRNAVPKDGGK 281

[Close]

BLAST of GR395758 vs. SwissProt
Match: COOMT_COPJA (Columbamine O-methyltransferase OS=Coptis japonica PE=1 SV=1)

HSP 1 Score: 43.8986 bits (102), Expect = 1.145e-8
Identity = 21/66 (31.82%), Postives = 41/66 (62.12%), Query Frame = 2

Query:  104 GKVIVIGMVINEKKDENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            GKVI++ +V+ + ++    T  +L M ++M  ++GKER +KEW+ L  +A F  +++ P+    S+
Sbjct:  282 GKVILVEIVV-DTENLPLFTSARLSMGMDMMLMSGKERTKKEWEDLLRKANFTSHQVIPIMAIESI 346

HSP 2 Score: 36.1946 bits (82), Expect = 1.145e-8
Identity = 16/32 (50.00%), Postives = 21/32 (65.62%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKK 102
            + +K  LHN+ D DCI+IL   KEA+ S G K
Sbjct:  252 ILLKSTLHNYEDDDCIKILNIAKEALPSTGGK 283

[Close]

BLAST of GR395758 vs. SwissProt
Match: EOMT1_OCIBA (Eugenol O-methyltransferase OS=Ocimum basilicum GN=EOMT1 PE=1 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.490e-7
Identity = 29/81 (35.80%), Postives = 47/81 (58.02%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMAC-LNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L+ ++K    +   GKVI+I +V+    D +++ + +L  D+ M C  N KER   EW+KL  +A F+ YK++P  G  SL
Sbjct:  272 LKILKKCKDAVVMGGKVIIIDVVVGVNHDIDEVLEDQLHFDMAMMCYFNAKERTMSEWEKLIYDAGFKSYKLTPAFGVRSL 352

[Close]

BLAST of GR395758 vs. SwissProt
Match: COMT1_EUCGU (Caffeic acid 3-O-methyltransferase OS=Eucalyptus gunnii GN=OMT PE=2 SV=1)

HSP 1 Score: 42.743 bits (99), Expect = 9.374e-7
Identity = 15/37 (40.54%), Postives = 25/37 (67.57%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKKRKSDC 117
            +FMKWI H+W+D  C + L+ C +A+ + GK   ++C
Sbjct:  263 IFMKWICHDWSDDHCAKFLKNCYDALPNIGKVIVAEC 299

HSP 2 Score: 30.8018 bits (68), Expect = 9.374e-7
Identity = 21/59 (35.59%), Postives = 32/59 (54.24%), Query Frame = 2

Query:  104 GKVIVIGMVINEKKDENQITQIKLLMDVNMACLN--GKERNEKEWKKLFIEARFQDYKI 274
            GKVIV   V+    D +  T+  + +D  M   N  GKER +KE++ L   A FQ +++
Sbjct:  292 GKVIVAECVLPVYPDTSLATKNVIHIDCIMLAHNPGGKERTQKEFETLAKGAGFQGFQV 350

[Close]

BLAST of GR395758 vs. TrEMBL
Match: Q1EDY7_MEDTR (SAM dependent isoflavone 7-O-methyltransferase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.821e-29
Identity = 65/80 (81.25%), Postives = 72/80 (90.00%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L+  ++ +   GKKGKVI+I MVINEKKDENQ+TQIKLLMDVNMACLNGKERNE+EWKKLFIEA FQDYKISPLTGFLSL
Sbjct:  268 LKKCKEAVTNDGKKGKVIIIDMVINEKKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQDYKISPLTGFLSL 347

HSP 2 Score: 60.4622 bits (145), Expect = 1.500e-7
Identity = 25/34 (73.53%), Postives = 31/34 (91.18%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKKRK 108
            + +K+ILHNWTDKDC RIL+KCKEAVT++GKK K
Sbjct:  250 VLLKYILHNWTDKDCTRILKKCKEAVTNDGKKGK 283

[Close]

BLAST of GR395758 vs. TrEMBL
Match: O22555_MEDSA (O-methytransferase OS=Medicago sativa GN=iomt2003 PE=2 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 3.797e-27
Identity = 61/80 (76.25%), Postives = 70/80 (87.50%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L+  ++ +   GK+GKV +I MVI+EKKDENQ+TQIKLLMDVNMACLNGKERNE+EWKKLFIEA FQ YKISPLTGFLSL
Sbjct:  259 LKKCKEAVTNDGKRGKVTIIDMVIDEKKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSL 338

[Close]

BLAST of GR395758 vs. TrEMBL
Match: Q06YR3_MEDTR (IOMT 3 OS=Medicago truncatula PE=1 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 2.084e-25
Identity = 57/80 (71.25%), Postives = 69/80 (86.25%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L+  ++ +   GK+GKVIVI MVINEKKDENQ+TQIKLLM+V ++C+NGKERNE+EWKKLFIEA FQDYKISP TG +SL
Sbjct:  273 LKKCKEAVTSDGKRGKVIVIDMVINEKKDENQLTQIKLLMNVTISCVNGKERNEEEWKKLFIEAGFQDYKISPFTGLMSL 352

[Close]

BLAST of GR395758 vs. TrEMBL
Match: Q84KK5_GLYEC (S-adenosyl-L-methionine: daidzein 7-0-methyltransferase OS=Glycyrrhiza echinata GN=D7OMT PE=2 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 1.351e-24
Identity = 57/80 (71.25%), Postives = 68/80 (85.00%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L   ++ +   G+KGKVI+I MVINE +DE++IT  KLLMDVNMACLNGKER+E+EWKKLFIEA F+DYKISPLTGFLSL
Sbjct:  273 LEKCKEAVSSDGEKGKVIIIEMVINENQDEHEITGTKLLMDVNMACLNGKERSEEEWKKLFIEAGFRDYKISPLTGFLSL 352

HSP 2 Score: 60.8474 bits (146), Expect = 1.149e-7
Identity = 25/34 (73.53%), Postives = 30/34 (88.24%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKKRK 108
            + +KWILHNWTD DC RILEKCKEAV+S+G+K K
Sbjct:  255 VLLKWILHNWTDNDCRRILEKCKEAVSSDGEKGK 288

[Close]

BLAST of GR395758 vs. TrEMBL
Match: Q06YR4_MEDTR (IOMT 2 OS=Medicago truncatula PE=4 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 3.930e-24
Identity = 54/80 (67.50%), Postives = 69/80 (86.25%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L+  ++ +   GK+GKVIVI MVINEKK+EN++TQIKLLM+V ++CLNGKERNE+EWKKLF+EA F++YKISP TG LSL
Sbjct:  273 LKKCKEAVTTEGKRGKVIVIDMVINEKKEENELTQIKLLMNVTISCLNGKERNEEEWKKLFMEAGFENYKISPCTGLLSL 352

HSP 2 Score: 60.077 bits (144), Expect = 1.959e-7
Identity = 23/34 (67.65%), Postives = 31/34 (91.18%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKKRK 108
            + +KWILH+WTD+DCI+IL+KCKEAVT+ GK+ K
Sbjct:  255 VLLKWILHDWTDRDCIKILKKCKEAVTTEGKRGK 288

[Close]

BLAST of GR395758 vs. TrEMBL
Match: C6TDI4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.950e-23
Identity = 50/80 (62.50%), Postives = 70/80 (87.50%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L+N ++ +   GK+GKVI+I +VINEK+DE+++T++KL+MD+ MAC+NGKERNE+EWKKLF+EA FQDYKI PLT +LS+
Sbjct:  269 LKNCKEAISNNGKRGKVIIIDVVINEKEDEHKVTELKLVMDITMACVNGKERNEEEWKKLFMEAGFQDYKIFPLTKYLSV 348

HSP 2 Score: 61.2326 bits (147), Expect = 8.795e-8
Identity = 23/35 (65.71%), Postives = 31/35 (88.57%), Query Frame = 1

Query:    4 VLFMKWILHNWTDKDCIRILEKCKEAVTSNGKKRK 108
            V+ +KWILHNW DKDCI+IL+ CKEA+++NGK+ K
Sbjct:  250 VILLKWILHNWIDKDCIKILKNCKEAISNNGKRGK 284

[Close]

BLAST of GR395758 vs. TrEMBL
Match: C6TEX3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 2.547e-23
Identity = 55/81 (67.90%), Postives = 68/81 (83.95%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMAC-LNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L N ++ +    K GKVIVI +VINEKKDE++IT++KLLMD+NMAC LNGKER E++WKKLF+EA FQ YKISPLTG+LSL
Sbjct:  269 LENCKEAISSKSKTGKVIVIDVVINEKKDEHEITRLKLLMDLNMACLLNGKERREEDWKKLFVEAGFQSYKISPLTGYLSL 349

[Close]

BLAST of GR395758 vs. TrEMBL
Match: O24249_PRUAR (Methyltransferase OS=Prunus armeniaca PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.146e-22
Identity = 35/70 (50.00%), Postives = 48/70 (68.57%), Query Frame = 2

Query:   95 GKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLN-GKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            GKKGKVI++ M +  K  + +  + +L  D++M  ++ GKERNEKEW KLF +A F  YKI+PL GF SL
Sbjct:  280 GKKGKVIIVEMTVENKNTDKESGETQLFFDMHMMVMSTGKERNEKEWAKLFSDAGFSQYKITPLFGFKSL 349

HSP 2 Score: 57.3806 bits (137), Expect = 2.146e-22
Identity = 21/34 (61.76%), Postives = 30/34 (88.24%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKKRK 108
            +F+KWILH+W+D++C++ILE+CK AVT  GKK K
Sbjct:  251 IFLKWILHDWSDEECVKILERCKAAVTREGKKGK 284

[Close]

BLAST of GR395758 vs. TrEMBL
Match: C6TAY1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 3.114e-21
Identity = 51/81 (62.96%), Postives = 64/81 (79.01%), Query Frame = 2

Query:   62 LRNVRKLLQVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMAC-LNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            L N ++ +    K GKV+VI  VINE KDE Q+T++KLLMDV+MAC +NGKER E++WKKLF+EA FQ YKISP TG+LSL
Sbjct:  273 LENCKEAISGESKTGKVVVIDTVINENKDERQVTELKLLMDVHMACIINGKERKEEDWKKLFMEAGFQSYKISPFTGYLSL 353

[Close]

BLAST of GR395758 vs. TrEMBL
Match: Q8L5K8_ROSHC (Orcinol O-methyltransferase OS=Rosa hybrid cultivar GN=OOMT1 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 4.411e-19
Identity = 35/69 (50.72%), Postives = 49/69 (71.01%), Query Frame = 2

Query:   98 KKGKVIVIGMVINEKKDENQITQIKLLMDV-NMACLNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            KKGKVI+I M++  +K + +  + +L  D+  MA + GKERNEKEW KLF +A F DYKI+P++G  SL
Sbjct:  294 KKGKVIIIDMMMENQKGDEESIETQLFFDMLMMALVGGKERNEKEWAKLFTDAGFSDYKITPISGLRSL 362

HSP 2 Score: 46.595 bits (109), Expect = 4.411e-19
Identity = 17/34 (50.00%), Postives = 27/34 (79.41%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGKKRK 108
            + +KWILH+W+D++CI+ILE+ + A+T   KK K
Sbjct:  264 VLLKWILHDWSDEECIKILERSRVAITGKEKKGK 297

[Close]

BLAST of GR395758 vs. TAIR peptide
Match: AT4G35160.1 (| Symbols: | O-methyltransferase family protein | chr4:16730989-16732808 REVERSE LENGTH=382)

HSP 1 Score: 51.6026 bits (122), Expect = 2.220e-12
Identity = 19/29 (65.52%), Postives = 25/29 (86.21%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSN 93
            +F+KW+LH+W DKDCI+IL+ CKEAV  N
Sbjct:  272 IFIKWVLHDWGDKDCIKILKNCKEAVPPN 300

HSP 2 Score: 37.7354 bits (86), Expect = 2.220e-12
Identity = 23/75 (30.67%), Postives = 38/75 (50.67%), Query Frame = 2

Query:  104 GKVIVIGMVINEKKD-------ENQITQIKLLMDVNMAC--LNGKERNEKEWKKLFIEARFQDYKISPLTGFLSL 301
            GKV+++  VI E K        + ++  ++L++D+ M      GKER  KEW  +  EA F  Y++  +    SL
Sbjct:  302 GKVLIVESVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSL 376

[Close]

BLAST of GR395758 vs. TAIR peptide
Match: AT1G33030.1 (| Symbols: | O-methyltransferase family protein | chr1:11964885-11966212 REVERSE LENGTH=352)

HSP 1 Score: 46.9802 bits (110), Expect = 3.246e-9
Identity = 16/31 (51.61%), Postives = 24/31 (77.42%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGK 99
            +FMKW+LH+W D  C++IL  C +++ SNGK
Sbjct:  249 IFMKWMLHSWDDDHCVKILSNCYQSLPSNGK 279

HSP 2 Score: 31.5722 bits (70), Expect = 3.246e-9
Identity = 21/65 (32.31%), Postives = 35/65 (53.85%), Query Frame = 2

Query:   86 QVMGKKGKVIVIGMVINEKKDENQITQIKLLMDVNMACLN--GKERNEKEWKKLFIEARFQDYKI 274
            Q +   GKVIV+ MVI E   +  + +     ++ M  +N  GKER +KE++ L   A F + ++
Sbjct:  272 QSLPSNGKVIVVDMVIPEFPGDTLLDRSLFQFELFMMNMNPSGKERTKKEFEILARLAGFSNVQV 336

[Close]

BLAST of GR395758 vs. TAIR peptide
Match: AT1G63140.2 (| Symbols: | O-methyltransferase family protein | chr1:23417517-23418875 FORWARD LENGTH=381)

HSP 1 Score: 45.4394 bits (106), Expect = 1.216e-7
Identity = 16/31 (51.61%), Postives = 25/31 (80.65%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGK 99
            +FMKWILH+WTD+DC++IL+   +++   GK
Sbjct:  279 IFMKWILHDWTDEDCVKILKNYWKSLPEKGK 309

HSP 2 Score: 27.7202 bits (60), Expect = 1.216e-7
Identity = 19/57 (33.33%), Postives = 34/57 (59.65%), Query Frame = 2

Query:   98 KKGKVIVIGMVINEKKDENQI-TQIKLLMDVNMACLN--GKERNEKEWKKLFIEARF 259
            +KGKVI++ +V  E+   N I + I   MD+ M  ++  GKER+  +++ L  ++ F
Sbjct:  306 EKGKVIIVEVVTPEEPKINDISSNIVFGMDMLMLAVSSGGKERSLSQFETLASDSGF 362

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BLAST of GR395758 vs. TAIR peptide
Match: AT1G62900.1 (| Symbols: | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | chr1:23297792-23298536 FORWARD LENGTH=205)

HSP 1 Score: 45.4394 bits (106), Expect = 1.253e-7
Identity = 16/31 (51.61%), Postives = 25/31 (80.65%), Query Frame = 1

Query:    7 LFMKWILHNWTDKDCIRILEKCKEAVTSNGK 99
            +FMKWILH+WTD+DC++IL+   +++   GK
Sbjct:  103 IFMKWILHDWTDEDCVKILKNYWKSLPEKGK 133

HSP 2 Score: 27.7202 bits (60), Expect = 1.253e-7
Identity = 19/57 (33.33%), Postives = 34/57 (59.65%), Query Frame = 2

Query:   98 KKGKVIVIGMVINEKKDENQI-TQIKLLMDVNMACLN--GKERNEKEWKKLFIEARF 259
            +KGKVI++ +V  E+   N I + I   MD+ M  ++  GKER+  +++ L  ++ F
Sbjct:  130 EKGKVIIVEVVTPEEPKINDISSNIVFGMDMLMLAVSSGGKERSLSQFETLASDSGF 186

The following BLAST results are available for this feature:

BLAST of GR395758 vs. SwissProt
BLAST of GR395758 vs. TrEMBL
BLAST of GR395758 vs. TAIR peptide

BLAST of GR395758 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
7OMT9_MEDSA	6.735e-29	77.50	Isoflavone-7-O-methyltransferase 9 OS=Medicago sat...	[more]
7OMT6_MEDSA	2.559e-28	76.25	Isoflavone-7-O-methyltransferase 6 OS=Medicago sat...	[more]
7OMT8_MEDSA	7.446e-28	75.00	Isoflavone-7-O-methyltransferase 8 OS=Medicago sat...	[more]
ZRP4_MAIZE	4.190e-14	36.25	O-methyltransferase ZRP4 OS=Zea mays GN=ZRP4 PE=2 ...	[more]
6OMT_COPJA	5.450e-14	39.13	(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis...	[more]
4OMT_COPJA	6.809e-9	29.85	3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltran...	[more]
COOMT_COPJA	1.145e-8	31.82	Columbamine O-methyltransferase OS=Coptis japonica...	[more]
EOMT1_OCIBA	2.490e-7	35.80	Eugenol O-methyltransferase OS=Ocimum basilicum GN...	[more]
COMT1_EUCGU	9.374e-7	40.54	Caffeic acid 3-O-methyltransferase OS=Eucalyptus g...	[more]

BLAST of GR395758 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q1EDY7_MEDTR	1.821e-29	81.25	SAM dependent isoflavone 7-O-methyltransferase OS=...	[more]
O22555_MEDSA	3.797e-27	76.25	O-methytransferase OS=Medicago sativa GN=iomt2003 ...	[more]
Q06YR3_MEDTR	2.084e-25	71.25	IOMT 3 OS=Medicago truncatula PE=1 SV=1	[more]
Q84KK5_GLYEC	1.351e-24	71.25	S-adenosyl-L-methionine: daidzein 7-0-methyltransf...	[more]
Q06YR4_MEDTR	3.930e-24	67.50	IOMT 2 OS=Medicago truncatula PE=4 SV=1	[more]
C6TDI4_SOYBN	1.950e-23	62.50	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TEX3_SOYBN	2.547e-23	67.90	Putative uncharacterized protein OS=Glycine max PE...	[more]
O24249_PRUAR	2.146e-22	50.00	Methyltransferase OS=Prunus armeniaca PE=2 SV=1	[more]
C6TAY1_SOYBN	3.114e-21	62.96	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q8L5K8_ROSHC	4.411e-19	50.72	Orcinol O-methyltransferase OS=Rosa hybrid cultiva...	[more]

BLAST of GR395758 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT4G35160.1	2.220e-12	65.52	\| Symbols: \| O-methyltransferase family protein \|...	[more]
AT1G33030.1	3.246e-9	51.61	\| Symbols: \| O-methyltransferase family protein \|...	[more]
AT1G63140.2	1.216e-7	51.61	\| Symbols: \| O-methyltransferase family protein \|...	[more]
AT1G62900.1	1.253e-7	51.61	\| Symbols: \| S-adenosyl-L-methionine-dependent me...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 62..78 score
None	No IPR available	GENE3D	3.40.50.150	no description	coord: 6..82 score: 6.8
None	No IPR available	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 7..87 score: 1.3
None	No IPR available	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 2..83 score: 8.4
None	No IPR available	SEG	seg	seg	coord: 37..48 score
None	No IPR available	SEG	seg	seg	coord: 25..36 score
None	No IPR available	SEG	seg	seg	coord: 2..13 score
None	No IPR available	SEG	seg	seg	coord: 9..24 score
IPR001077	O-methyltransferase, family 2	PFAM	PF00891	Methyltransf_2	coord: 3..63 score: 1.5

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR395758 ID=GR395758; Name=GR395758; organism=Cicer arietinum; type=EST; length=689bp
TTTGTTTTATTTATGAAGTGGATTTTACATAATTGGACTGATAAGGATTG
CATAAGGATACTTGAGAAATGTAAGGAAGCTGTTACAAGTAATGGGAAAA
AAAGGAAAAGTGATTGTGATAGGTATGGTGATAAATGAAAAGAAAGATGA
GAACCAAATTACTCAAATAAAGCTCCTTATGGATGTGAACATGGCATGTC
TTAATGGAAAAGAGAGAAATGAGAAAGAATGGAAGAAACTTTTCATTGAA
GCACGCTTCCAAGACTATAAGATATCTCCTTTGACTGGATTTTTGTCCCT
CTACCGAAATCTATCTTTACAATAGTAGTGCTTTGTGAATTTGTGATCTA
TTTAATATTTTACATGTTCATCAATTATCACCCGTTTTGATCCACTCTAA
TACTTACTTTCACCAAAATTCCGGCCCCCTCACCCCCTACACTATATTTA
ACAACCCATTTTTCTCCCCTTTCAATTCCCCCCACCTTTATATACTCTCC
ACATCTCCTAGATCCTCCTTGTATGACCCCTACTAAATCTAGACACAACC
CAACTACAAGAATTTATTATTGTACCATAATATCCCTACTATTACTCCCA
TTCCCTCTATACTTTTCTCTCACACAATCTTACCCCCCATAATATATTTT
TATTTTCCTACTACTTCTATCCCCCCCTCTTATATCTCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008171	O-methyltransferase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR001077	O_MeTrfase_dom

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