GR408876

EST Overview


Name	GR408876
Unique Name	GR408876
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	579

Libraries


Library Name	Type
LIBEST_024777 ICC1882 dehydration stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR408876 vs. SwissProt
Match: ISPG_PARUW (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Protochlamydia amoebophila (strain UWE25) GN=ispG PE=3 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 1.091e-33
Identity = 64/93 (68.82%), Postives = 76/93 (81.72%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDP 281
            CPSCGRTLF+LQ ++  I+ +TSHLPGV IAIMGCIVNGPGEMADADFGYVG  PGKIDLYVGK  V++ I    A D L++LI+ HGRW++P
Sbjct:  557 CPSCGRTLFNLQDVTKRIQSRTSHLPGVKIAIMGCIVNGPGEMADADFGYVGSKPGKIDLYVGKECVEKDIDFADADDRLVNLIRAHGRWIEP 649

[Close]

BLAST of GR408876 vs. SwissProt
Match: ISPG_AKKM8 (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Akkermansia muciniphila (strain ATCC BAA-835) GN=ispG PE=3 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 3.173e-33
Identity = 65/93 (69.89%), Postives = 76/93 (81.72%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDP 281
            CPSCGRTL+++Q  +A IR+ T HL GV IA+MGCIVNGPGEMADADFGYVGGAP KI+LYV  T VK  I  E+A + L+DLIKE+GRWVDP
Sbjct:  492 CPSCGRTLYNIQEATARIRKATEHLKGVKIAVMGCIVNGPGEMADADFGYVGGAPNKINLYVKHTPVKFNIPQEEAVERLVDLIKEYGRWVDP 584

[Close]

BLAST of GR408876 vs. SwissProt
Match: ISPG_CHLPN (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Chlamydia pneumoniae GN=ispG PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.474e-30
Identity = 65/100 (65.00%), Postives = 75/100 (75.00%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE*KL 302
            CP CGRTLFDL+ ++  IR++T HLPG+ IAIMGCIVNGPGEMADADFG+VG   G IDLYV  T VK  I ME A + LI L++EHG W DP  EE KL
Sbjct:  514 CPMCGRTLFDLEEVTTRIRKRTQHLPGLKIAIMGCIVNGPGEMADADFGFVGSKTGMIDLYVKHTCVKAHIPMEDAEEELIRLLQEHGVWKDP--EETKL 611

[Close]

BLAST of GR408876 vs. SwissProt
Match: ISPG_LEPIN (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Leptospira interrogans GN=ispG PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.514e-30
Identity = 59/93 (63.44%), Postives = 71/93 (76.34%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDP 281
            CPSCGRTLFDLQ  +A I+ +T HL GV IA+MGCIVNGPGEMADADFGYVG  PGK+ LY GK +V + +  E A + L+ LIK++G W DP
Sbjct:  568 CPSCGRTLFDLQETTARIKSRTGHLKGVKIAVMGCIVNGPGEMADADFGYVGAGPGKVHLYRGKEIVLKNVPSEIADEKLVQLIKDNGLWQDP 660

[Close]

BLAST of GR408876 vs. SwissProt
Match: ISPG_LEPBL (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Leptospira borgpetersenii serovar Hardjo-bovis (strain L550) GN=ispG PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 4.289e-30
Identity = 58/92 (63.04%), Postives = 71/92 (77.17%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVD 278
            CPSCGRTLF+LQ  +A I+ +T HL GV IA+MGCIVNGPGEMADADFGYVG  PGK+ LY GK +V + +  E A + L++LIK+HG W D
Sbjct:  568 CPSCGRTLFNLQETTARIKSRTGHLKGVKIAVMGCIVNGPGEMADADFGYVGAGPGKVHLYRGKEIVMKNVPSEVADEKLVELIKKHGLWQD 659

[Close]

BLAST of GR408876 vs. SwissProt
Match: ISPG_LEPBJ (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) GN=ispG PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 4.289e-30
Identity = 58/92 (63.04%), Postives = 71/92 (77.17%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVD 278
            CPSCGRTLF+LQ  +A I+ +T HL GV IA+MGCIVNGPGEMADADFGYVG  PGK+ LY GK +V + +  E A + L++LIK+HG W D
Sbjct:  568 CPSCGRTLFNLQETTARIKSRTGHLKGVKIAVMGCIVNGPGEMADADFGYVGAGPGKVHLYRGKEIVMKNVPSEVADEKLVELIKKHGLWQD 659

[Close]

BLAST of GR408876 vs. SwissProt
Match: ISPG_CHLAB (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Chlamydophila abortus GN=ispG PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 9.554e-30
Identity = 60/93 (64.52%), Postives = 69/93 (74.19%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDP 281
            CP CGRTLFDL  ++  I E+T HL G+ IA+MGCIVNGPGEMADADFG+VG   G +DLYV  T VK  I MEQA D L+ L+KEHG W DP
Sbjct:  513 CPGCGRTLFDLPEVTKRIHERTQHLVGLKIAVMGCIVNGPGEMADADFGFVGSKTGMVDLYVKHTCVKAHIPMEQAEDELVLLLKEHGVWKDP 605

[Close]

BLAST of GR408876 vs. SwissProt
Match: ISPG_CHLTR (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Chlamydia trachomatis GN=ispG PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.248e-29
Identity = 61/94 (64.89%), Postives = 70/94 (74.47%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPG-VSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDP 281
            CP CGRTLFDL  +S  IRE+T HLPG + IA+MGCIVNGPGEMADADFGYVG  PG IDLYV    VK  I +E A + L+ L+KEHG W +P
Sbjct:  508 CPGCGRTLFDLLAVSQRIRERTQHLPGGLKIAVMGCIVNGPGEMADADFGYVGSKPGMIDLYVKHKCVKSCIPIENAEEELVQLLKEHGVWKEP 601

[Close]

BLAST of GR408876 vs. SwissProt
Match: ISPG_CHLTB (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Chlamydia trachomatis (strain L2b/UCH-1/proctitis) GN=ispG PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.248e-29
Identity = 61/94 (64.89%), Postives = 70/94 (74.47%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPG-VSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDP 281
            CP CGRTLFDL  +S  IRE+T HLPG + IA+MGCIVNGPGEMADADFGYVG  PG IDLYV    VK  I +E A + L+ L+KEHG W +P
Sbjct:  508 CPGCGRTLFDLLAVSQRIRERTKHLPGGLKIAVMGCIVNGPGEMADADFGYVGSKPGMIDLYVKHKCVKSCIPIENAEEELVQLLKEHGVWKEP 601

[Close]

BLAST of GR408876 vs. SwissProt
Match: ISPG_CHLTA (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Chlamydia trachomatis (strain A/HAR-13 / ATCC VR-571B) GN=ispG PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.248e-29
Identity = 61/94 (64.89%), Postives = 70/94 (74.47%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPG-VSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDP 281
            CP CGRTLFDL  +S  IRE+T HLPG + IA+MGCIVNGPGEMADADFGYVG  PG IDLYV    VK  I +E A + L+ L+KEHG W +P
Sbjct:  508 CPGCGRTLFDLLAVSQRIRERTQHLPGGLKIAVMGCIVNGPGEMADADFGYVGSKPGMIDLYVKHKCVKSCIPIENAEEELVQLLKEHGVWKEP 601

[Close]

BLAST of GR408876 vs. TrEMBL
Match: D2D5E3_ARTAN ((E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase OS=Artemisia annua GN=HDS PE=2 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 7.491e-48
Identity = 93/97 (95.88%), Postives = 95/97 (97.94%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVV+RGIAME ATDALI LIK+HGRWVDPPVEE
Sbjct:  521 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVQRGIAMEGATDALIQLIKDHGRWVDPPVEE 617

[Close]

BLAST of GR408876 vs. TrEMBL
Match: D7SKY1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025182001 PE=4 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 4.856e-47
Identity = 91/97 (93.81%), Postives = 93/97 (95.88%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQ ISA+IREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAME ATDALI LIK+HGRWVDPP EE
Sbjct:  644 CPSCGRTLFDLQEISAQIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEHATDALIQLIKDHGRWVDPPAEE 740

[Close]

BLAST of GR408876 vs. TrEMBL
Match: A9ZN14_HEVBR (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Hevea brasiliensis GN=HbHDS PE=2 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 4.856e-47
Identity = 92/97 (94.85%), Postives = 93/97 (95.88%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQ ISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAME ATDALI LIK+HGRWVDPP EE
Sbjct:  644 CPSCGRTLFDLQDISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEGATDALIQLIKDHGRWVDPPAEE 740

[Close]

BLAST of GR408876 vs. TrEMBL
Match: A5BBY6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005654 PE=3 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 4.856e-47
Identity = 91/97 (93.81%), Postives = 93/97 (95.88%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQ ISA+IREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAME ATDALI LIK+HGRWVDPP EE
Sbjct:  644 CPSCGRTLFDLQEISAQIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEHATDALIQLIKDHGRWVDPPAEE 740

[Close]

BLAST of GR408876 vs. TrEMBL
Match: Q0PUV7_STERE ((E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase OS=Stevia rebaudiana GN=HDS PE=2 SV=2)

HSP 1 Score: 190.66 bits (483), Expect = 6.342e-47
Identity = 91/97 (93.81%), Postives = 95/97 (97.94%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQVISAEIR+KTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVV+RGIAME ATDALI LIK++GRWVDPPVEE
Sbjct:  644 CPSCGRTLFDLQVISAEIRDKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVQRGIAMENATDALIQLIKDNGRWVDPPVEE 740

[Close]

BLAST of GR408876 vs. TrEMBL
Match: Q84PA7_ORYSJ (Isoprenoid biosynthesis-like protein (Fragment) OS=Oryza sativa subsp. japonica PE=2 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 1.082e-46
Identity = 90/97 (92.78%), Postives = 94/97 (96.91%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQ +SA+IREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVV+RGIAME ATDALI LIK+HGRWVDPPVEE
Sbjct:  512 CPSCGRTLFDLQEVSAQIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVQRGIAMEGATDALIQLIKDHGRWVDPPVEE 608

[Close]

BLAST of GR408876 vs. TrEMBL
Match: Q6K8J4_ORYSJ (Os02g0603800 protein OS=Oryza sativa subsp. japonica GN=OJ1669_F01.30 PE=2 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 1.082e-46
Identity = 90/97 (92.78%), Postives = 94/97 (96.91%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQ +SA+IREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVV+RGIAME ATDALI LIK+HGRWVDPPVEE
Sbjct:  648 CPSCGRTLFDLQEVSAQIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVQRGIAMEGATDALIQLIKDHGRWVDPPVEE 744

[Close]

BLAST of GR408876 vs. TrEMBL
Match: B8AF20_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_07973 PE=3 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 1.082e-46
Identity = 90/97 (92.78%), Postives = 94/97 (96.91%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQ +SA+IREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVV+RGIAME ATDALI LIK+HGRWVDPPVEE
Sbjct:  648 CPSCGRTLFDLQEVSAQIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVQRGIAMEGATDALIQLIKDHGRWVDPPVEE 744

[Close]

BLAST of GR408876 vs. TrEMBL
Match: A9CBE3_GINBI ((E)-4-hydroxy-3-methyl-but-2-enyl diphosphate synthase OS=Ginkgo biloba PE=2 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 1.082e-46
Identity = 90/97 (92.78%), Postives = 94/97 (96.91%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQ ISAEIREKT+HLPGVSIAIMGCIVNGPGEMADADFGYVGG+PGKIDLYVGK VVKRGIAM+QATDALI LIK+HGRWVDPPVEE
Sbjct:  645 CPSCGRTLFDLQEISAEIREKTAHLPGVSIAIMGCIVNGPGEMADADFGYVGGSPGKIDLYVGKEVVKRGIAMDQATDALIQLIKDHGRWVDPPVEE 741

[Close]

BLAST of GR408876 vs. TrEMBL
Match: Q9FF59_ARATH (GcpE protein OS=Arabidopsis thaliana PE=3 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 3.147e-46
Identity = 90/97 (92.78%), Postives = 93/97 (95.88%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQ ISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGG+PGKIDLYVGKTVVKRGIAM +ATDALI LIKEHGRWVDPPV +
Sbjct:  619 CPSCGRTLFDLQEISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGSPGKIDLYVGKTVVKRGIAMTEATDALIGLIKEHGRWVDPPVAD 715

[Close]

BLAST of GR408876 vs. TAIR peptide
Match: AT5G60600.2 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363274 FORWARD LENGTH=740)

HSP 1 Score: 188.348 bits (477), Expect = 2.400e-48
Identity = 90/97 (92.78%), Postives = 93/97 (95.88%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQ ISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGG+PGKIDLYVGKTVVKRGIAM +ATDALI LIKEHGRWVDPPV +
Sbjct:  643 CPSCGRTLFDLQEISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGSPGKIDLYVGKTVVKRGIAMTEATDALIGLIKEHGRWVDPPVAD 739

[Close]

BLAST of GR408876 vs. TAIR peptide
Match: AT5G60600.1 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363274 FORWARD LENGTH=741)

HSP 1 Score: 188.348 bits (477), Expect = 2.400e-48
Identity = 90/97 (92.78%), Postives = 93/97 (95.88%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKTVVKRGIAMEQATDALIDLIKEHGRWVDPPVEE 293
            CPSCGRTLFDLQ ISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGG+PGKIDLYVGKTVVKRGIAM +ATDALI LIKEHGRWVDPPV +
Sbjct:  644 CPSCGRTLFDLQEISAEIREKTSHLPGVSIAIMGCIVNGPGEMADADFGYVGGSPGKIDLYVGKTVVKRGIAMTEATDALIGLIKEHGRWVDPPVAD 740

[Close]

BLAST of GR408876 vs. TAIR peptide
Match: AT5G60600.3 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363019 FORWARD LENGTH=717)

HSP 1 Score: 62.003 bits (149), Expect = 4.206e-14
Identity = 28/30 (93.33%), Postives = 29/30 (96.67%), Query Frame = 3

Query:    3 CPSCGRTLFDLQVISAEIREKTSHLPGVSI 92
            CPSCGRTLFDLQ ISAEIREKTSHLPGVS+
Sbjct:  644 CPSCGRTLFDLQEISAEIREKTSHLPGVSV 673

HSP 2 Score: 32.7278 bits (73), Expect = 4.206e-14
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = 2

Query:   89 DCNHGMHCKWSGRDG*C 139
            D NHGMHC+W+ R+G C
Sbjct:  701 DRNHGMHCEWTRRNGRC 717

The following BLAST results are available for this feature:

BLAST of GR408876 vs. SwissProt
BLAST of GR408876 vs. TrEMBL
BLAST of GR408876 vs. TAIR peptide

BLAST of GR408876 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ISPG_PARUW	1.091e-33	68.82	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
ISPG_AKKM8	3.173e-33	69.89	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
ISPG_CHLPN	1.474e-30	65.00	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
ISPG_LEPIN	2.514e-30	63.44	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
ISPG_LEPBL	4.289e-30	63.04	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
ISPG_LEPBJ	4.289e-30	63.04	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
ISPG_CHLAB	9.554e-30	64.52	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
ISPG_CHLTR	1.248e-29	64.89	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
ISPG_CHLTB	1.248e-29	64.89	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
ISPG_CHLTA	1.248e-29	64.89	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]

BLAST of GR408876 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D2D5E3_ARTAN	7.491e-48	95.88	(E)-4-hydroxy-3-methylbut-2-enyl diphosphate synth...	[more]
D7SKY1_VITVI	4.856e-47	93.81	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9ZN14_HEVBR	4.856e-47	94.85	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
A5BBY6_VITVI	4.856e-47	93.81	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q0PUV7_STERE	6.342e-47	93.81	(E)-4-hydroxy-3-methylbut-2-enyl diphosphate synth...	[more]
Q84PA7_ORYSJ	1.082e-46	92.78	Isoprenoid biosynthesis-like protein (Fragment) OS...	[more]
Q6K8J4_ORYSJ	1.082e-46	92.78	Os02g0603800 protein OS=Oryza sativa subsp. japoni...	[more]
B8AF20_ORYSI	1.082e-46	92.78	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A9CBE3_GINBI	1.082e-46	92.78	(E)-4-hydroxy-3-methyl-but-2-enyl diphosphate synt...	[more]
Q9FF59_ARATH	3.147e-46	92.78	GcpE protein OS=Arabidopsis thaliana PE=3 SV=1	[more]

BLAST of GR408876 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G60600.2	2.400e-48	92.78	\| Symbols: GCPE, ISPG, CSB3, CLB4, HDS \| 4-hydroxy...	[more]
AT5G60600.1	2.400e-48	92.78	\| Symbols: GCPE, ISPG, CSB3, CLB4, HDS \| 4-hydroxy...	[more]
AT5G60600.3	4.206e-14	93.33	\| Symbols: GCPE, ISPG, CSB3, CLB4, HDS \| 4-hydroxy...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004588	4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, bacterial-type	PFAM	PF04551	GcpE	coord: 1..87 score: 2.6
None	No IPR available	SUPERFAMILY	56014	Sulfite reductase hemoprotein (SiRHP), domains 2 and 4	coord: 1..85 score: 1.6
None	No IPR available	SEG	seg	seg	coord: 26..39 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR408876 ID=GR408876; Name=GR408876; organism=Cicer arietinum; type=EST; length=579bp
CATGCCCATCGTGTGGCAGAACCTTGTTTGATCTTCAAGTAATAAGCGCA
GAAATCCGGGAGAAGACATCGCACCTCCCTGGTGTTTCGATTGCAATCAT
GGGATGCATTGTAAATGGTCCGGGAGAGATGGCTGATGCAGACTTTGGGT
ATGTGGGAGGTGCTCCTGGGAAGATTGACCTCTATGTTGGGAAGACTGTG
GTGAAGCGAGGGATTGCGATGGAGCAAGCGACCGATGCCTTGATCGATCT
AATAAAAGAGCATGGACGTTGGGTAGACCCTCCTGTAGAGGAGTAAAAGC
TACATCTCAATGTTCTGATTAGCCACCAATAAGGTCCTGTCACAAGGGAC
GTTGGGTGGATCCTCTAGGATGATCAATGTATTTAATATGTTATATGAAC
CTCAGAAAGTTTTATTTGCAATTTTCAACTTAGTTGGAAAATGCTGAAAT
TTTCTTTTTACAAAGATCACAAATAAAGTATTAGAGTGGAAAATTCTTCA
GCTGAATCATGTATACAATAAAAAGTGCATTGCTTTGCCCCCATCAATGA
TGATGATATTATTATAGAGAAATTCTCCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0016114	terpenoid biosynthetic process
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0046429	4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR004588	IspG_bac-typ

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