EH059303

EST Overview


Name	EH059303
Unique Name	EH059303
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	650

Libraries


Library Name	Type
LIBEST_020668 subtracted cDNA library of drought stressed chickpea	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of EH059303 vs. SwissProt
Match: AGAL_CYATE (Alpha-galactosidase OS=Cyamopsis tetragonoloba PE=1 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 1.063e-49
Identity = 98/131 (74.81%), Postives = 113/131 (86.26%), Query Frame = -2

Query:  188 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTPQS 580
            F   AL KAP+ +  +I+A D+TT ELI+N EV+AVNQD  GVQGKKVKS +DLEVWAGPLS+NKVAVILWNRSSS ATVTASWSD+GL+ GT VDARDLW+HSTQS VSG+ISAE+ SHACKMYVLTP+S
Sbjct:  281 FSIWALAKAPLLVGCDIRAMDDTTHELISNAEVIAVNQDKLGVQGKKVKSTNDLEVWAGPLSDNKVAVILWNRSSSRATVTASWSDIGLQQGTTVDARDLWEHSTQSLVSGEISAEIDSHACKMYVLTPRS 411

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BLAST of EH059303 vs. SwissProt
Match: AGAL_COFAR (Alpha-galactosidase OS=Coffea arabica PE=1 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 3.207e-38
Identity = 79/130 (60.77%), Postives = 96/130 (73.85%), Query Frame = -2

Query:  191 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTPQ 580
            F   AL KAP+ I  +I++ D  T +L++N EV+AVNQD  GVQG KVK+  DLEVWAGPLS  +VAV LWNR SS AT+TA WSD+GL S  +V+ARDLW HST+ SV G ISA + +H  KMYVLTPQ
Sbjct:  249 FSIWALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGDLEVWAGPLSGKRVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAHSTEKSVKGQISAAVDAHDSKMYVLTPQ 378

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BLAST of EH059303 vs. SwissProt
Match: AGAL_ORYSJ (Alpha-galactosidase OS=Oryza sativa subsp. japonica GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 2.810e-34
Identity = 72/129 (55.81%), Postives = 93/129 (72.09%), Query Frame = -2

Query:  194 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTP 580
            F   AL KAP+ I  ++++    T  +++N EV+AVNQD+ GVQGKKV+S++ LEVWAGPLSNN+ AV+LWNR S  AT+TA WS++GL     V ARDLW HS+  +  G ISA +A H CKMYVLTP
Sbjct:  289 FSIWALAKAPLLIGCDVRSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQSDNGLEVWAGPLSNNRKAVVLWNRQSYQATITAHWSNIGLAGSVAVTARDLWAHSS-FAAQGQISASVAPHDCKMYVLTP 416

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BLAST of EH059303 vs. SwissProt
Match: AGAL_DICDI (Probable alpha-galactosidase OS=Dictyostelium discoideum GN=melA PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 5.509e-14
Identity = 47/140 (33.57%), Postives = 76/140 (54.29%), Query Frame = -2

Query:  194 DTE*IVPFQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKS-NSDLE-VWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAE--LASHACKMYVLTP 601
            +TE +  F   +L  AP+    ++   D  T+ ++T  EV+A+NQD  GVQG  VKS N  L+ +WA PLSN   AV+L+N  ++ AT+   W ++ +E    +  R+LW  +   + +    ++  +  H C M  LTP
Sbjct:  244 NTEYVSHFSLWSLLNAPLIAGCDLIDIDQETLSILTASEVIAINQDPLGVQGSLVKSYNGGLQQIWAKPLSNGARAVVLFNTDTNPATIELLWGNIWMEPSQQLSIRNLWTQTNLGTFTESYESDSLIPPHGCIMLTLTP 383

[Close]

BLAST of EH059303 vs. SwissProt
Match: MEL_ZYGCI (Alpha-galactosidase OS=Zygosaccharomyces cidri GN=MEL PE=3 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 4.517e-8
Identity = 31/110 (28.18%), Postives = 58/110 (52.73%), Query Frame = -2

Query:  311 NEQDTE*IVPFQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRG----------VQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLE 610
            N  D+E +  F   A+ K+P+ I  +I    ++++ + +NP V+A+NQD  G          V  K      ++++W+GPL N    V L N  ++  ++ ASW+D+ ++
Sbjct:  269 NMSDSEEVAHFSMWAIVKSPLIIGADIDDLKDSSLSVYSNPAVIAINQDVLGTPATRIWKYHVSDKDQYGEGEIQLWSGPLDNGDHVVALLNGGNNERSMNASWNDIFID 378

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BLAST of EH059303 vs. SwissProt
Match: AGALA_ASPNG (Alpha-galactosidase A OS=Aspergillus niger GN=aglA PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 4.995e-7
Identity = 39/125 (31.20%), Postives = 58/125 (46.40%), Query Frame = -2

Query:  185 APV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGL-ESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTPQSG 556
            AP+ I   I A     +  +TN  ++AVNQD    Q      +  L++    L+N    + + N+ ++  T       LGL E+     A DLWD  TQ  +S  I  ELASHA  ++ L+   G
Sbjct:  297 APLIISAYIPALSKDEIAFLTNEALIAVNQDPLAQQATLASRDDTLDILTRSLANGDRLLTVLNKGNTTVTRDIPVQWLGLTETDCTYTAEDLWDGKTQ-KISDHIKIELASHATAVFRLSLPQG 420

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BLAST of EH059303 vs. SwissProt
Match: AGALD_NEOFI (Probable alpha-galactosidase D OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN=aglD PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 6.523e-7
Identity = 37/133 (27.82%), Postives = 65/133 (48.87%), Query Frame = -2

Query:  200 FQHLALGKAPV*IVLNIQAKDNT----TVELITNPEVMAVNQDTRGVQG--KKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVL 580
            F   A+ K+P+ I   ++  D +    ++E++   +V+  NQD  GV    K+  S+   EVW+GPLS N+  V + N    +  +T    D+GL+   +  AR++W  +    V    +A +A H   +  L
Sbjct:  270 FSLWAILKSPLTIGAALKDDDTSINQASLEVLKQKDVIGFNQDALGVSASLKRRWSDEGYEVWSGPLSGNRTVVAVINWRDESRDLTLDLPDVGLQYAQV--ARNIWGKTVVRDVRTSYTAGVAGHGTMLLEL 400

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BLAST of EH059303 vs. SwissProt
Match: AGALD_ASPFU (Probable alpha-galactosidase D OS=Aspergillus fumigatus GN=aglD PE=3 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 6.523e-7
Identity = 36/126 (28.57%), Postives = 64/126 (50.79%), Query Frame = -2

Query:  221 FQHLALGKAPV*IVLNIQAKDNT----TVELITNPEVMAVNQDTRGVQG--KKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASH 580
            F   A+ K+P+ I   ++  D +    ++E++   +V+  NQD  GV    K+  S+   EVW+GPLS N+  V + N  + +  +T    D+GL+   +  AR++W  +    V    +A +A H
Sbjct:  270 FSLWAILKSPLTIGAALKDDDTSISQASLEVLKQKDVIGFNQDALGVSASLKRRWSDEGYEVWSGPLSGNRTVVAVINWRNESRDLTLDLPDVGLQYAQV--ARNIWGKTVVRDVRTSYTAGVAGH 393

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BLAST of EH059303 vs. SwissProt
Match: AGALD_ASPFC (Probable alpha-galactosidase D OS=Aspergillus fumigatus (strain CEA10 / CBS 144.89 / FGSC A1163) GN=aglD PE=3 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 6.523e-7
Identity = 36/126 (28.57%), Postives = 64/126 (50.79%), Query Frame = -2

Query:  221 FQHLALGKAPV*IVLNIQAKDNT----TVELITNPEVMAVNQDTRGVQG--KKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASH 580
            F   A+ K+P+ I   ++  D +    ++E++   +V+  NQD  GV    K+  S+   EVW+GPLS N+  V + N  + +  +T    D+GL+   +  AR++W  +    V    +A +A H
Sbjct:  270 FSLWAILKSPLTIGAALKDDDTSISQASLEVLKQKDVIGFNQDALGVSASLKRRWSDEGYEVWSGPLSGNRTVVAVINWRNESRDLTLDLPDVGLQYAQV--ARNIWGKTVVRDVRTSYTAGVAGH 393

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BLAST of EH059303 vs. SwissProt
Match: AGALA_ASPTN (Probable alpha-galactosidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN=aglA PE=3 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 6.523e-7
Identity = 34/124 (27.42%), Postives = 59/124 (47.58%), Query Frame = -2

Query:  185 APV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTPQSG 556
            AP+ I   I       +  +TN +++AV+QD    Q      + +++V    L++    V + NR +S+         LGL  G    AR+LWD  T+  +  D++  + SHA ++Y  T  +G
Sbjct:  285 APLIISAYIPGLSEEDIGYLTNRDLIAVDQDPLAQQATLASRDDEVDVLTRSLADGSRLVSVLNRGNSSVQRVIPLQWLGLNPGQRYQARNLWD-GTEKRIRKDLTVTVRSHATEIYKFTGSNG 407

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BLAST of EH059303 vs. TrEMBL
Match: Q41100_PHAVU (Alpha-galactosidase OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 1.444e-46
Identity = 96/128 (75.00%), Postives = 109/128 (85.16%), Query Frame = -2

Query:  197 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLT 580
            F   AL KAP+ I  +I+A D TT EL++N EV+AVNQD  GVQGKKVKSN+DLEVWAGPLSNN++AVILWNRSSS A VTASWSD+GL+ GT+VDARDLW HSTQSSVSG+ISAEL SHAC MYVLT
Sbjct:  296 FSIWALAKAPLLIGCDIRALDVTTKELLSNEEVIAVNQDKLGVQGKKVKSNNDLEVWAGPLSNNRLAVILWNRSSSKAKVTASWSDIGLKPGTLVDARDLWKHSTQSSVSGEISAELDSHACNMYVLT 423

[Close]

BLAST of EH059303 vs. TrEMBL
Match: Q39811_SOYBN (Alpha galactosidase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 2.464e-46
Identity = 96/129 (74.42%), Postives = 109/129 (84.50%), Query Frame = -2

Query:  194 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTP 580
            F   +L KAP+ I  +I+A D TT EL++N EV+AVNQD  GVQGKKVKS +DLEVWAGPLSNNKVAVILWNRSSS A VTASWSD+GL+ GT V+ARDLW HSTQSSVSG+ISAEL SHACKMYV+TP
Sbjct:  293 FSIWSLAKAPLLIGCDIRALDATTKELLSNKEVIAVNQDKLGVQGKKVKSTNDLEVWAGPLSNNKVAVILWNRSSSKAKVTASWSDIGLKPGTSVEARDLWAHSTQSSVSGEISAELDSHACKMYVVTP 421

[Close]

BLAST of EH059303 vs. TrEMBL
Match: C6TLN0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 2.464e-46
Identity = 96/129 (74.42%), Postives = 109/129 (84.50%), Query Frame = -2

Query:  194 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTP 580
            F   +L KAP+ I  +I+A D TT EL++N EV+AVNQD  GVQGKKVKS +DLEVWAGPLSNNKVAVILWNRSSS A VTASWSD+GL+ GT V+ARDLW HSTQSSVSG+ISAEL SHACKMYV+TP
Sbjct:  297 FSIWSLAKAPLLIGCDIRALDATTKELLSNKEVIAVNQDKLGVQGKKVKSTNDLEVWAGPLSNNKVAVILWNRSSSKAKVTASWSDIGLKPGTSVEARDLWAHSTQSSVSGEISAELDSHACKMYVVTP 425

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BLAST of EH059303 vs. TrEMBL
Match: B9N7Z5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_786149 PE=4 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 7.418e-43
Identity = 86/130 (66.15%), Postives = 103/130 (79.23%), Query Frame = -2

Query:  191 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTPQ 580
            F   AL KAP+ I  +++   + T+E+++N EV+AVNQD  GVQGKKVK+N DLEVWAGPLSNNK+AV+LWNR SS ATVTA WSD+GL+  T V+ARDLW HS Q SV G ISA+L SHACKMYVLTPQ
Sbjct:  238 FSIWALAKAPLLIGCDVRTMSDETIEILSNREVIAVNQDKLGVQGKKVKNNGDLEVWAGPLSNNKIAVVLWNRGSSRATVTAYWSDIGLDPTTTVNARDLWAHSNQPSVKGQISADLDSHACKMYVLTPQ 367

[Close]

BLAST of EH059303 vs. TrEMBL
Match: B9SI47_RICCO (Alpha-galactosidase/alpha-n-acetylgalactosaminidase, putative OS=Ricinus communis GN=RCOM_0612700 PE=4 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 1.827e-41
Identity = 88/130 (67.69%), Postives = 103/130 (79.23%), Query Frame = -2

Query:  194 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGL-ESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTP 580
            F   AL KAP+ I  +I++  N T E+++N EV+AVNQD  GVQGKKVK++ DLEVWAGPLSN KVA++LWNR SS ATVTA WSD+GL  S T+V ARDLW HSTQSSV G ISA+L SHACKMYVLTP
Sbjct:  284 FSIWALAKAPLLIGCDIRSMSNETYEILSNKEVIAVNQDKLGVQGKKVKNDGDLEVWAGPLSNRKVAIVLWNRGSSRATVTAYWSDIGLNNSTTMVHARDLWAHSTQSSVKGHISADLDSHACKMYVLTP 413

[Close]

BLAST of EH059303 vs. TrEMBL
Match: D7TXW6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_71.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034334001 PE=4 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 4.070e-41
Identity = 81/130 (62.31%), Postives = 103/130 (79.23%), Query Frame = -2

Query:  191 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTPQ 580
            F   AL KAP+ I  ++++ DN+T EL++N EV+AVNQD  GVQG+KVK + +LEVWAGPLS+NKVAV+LWNR SS A +TA WSD+GL    +VDARDLW HSTQSSV G + A++ SHACKMY+LTP+
Sbjct:  280 FSIWALAKAPLLIGCDVRSMDNSTFELLSNKEVIAVNQDELGVQGRKVKKDGNLEVWAGPLSDNKVAVVLWNRGSSQANMTAHWSDIGLSPSAVVDARDLWAHSTQSSVQGQLWAQVDSHACKMYILTPK 409

[Close]

BLAST of EH059303 vs. TrEMBL
Match: A5ALZ9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_000962 PE=4 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 4.500e-40
Identity = 80/130 (61.54%), Postives = 102/130 (78.46%), Query Frame = -2

Query:  191 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTPQ 580
            F   AL KAP+ I  ++++ DN+T EL++N EV+AVNQD  GVQG+KVK + +LEVWAGPLS+NKVAV+LWNR SS A +TA WSD+GL    +VDARDLW  STQSSV G + A++ SHACKMY+LTP+
Sbjct:  263 FSIWALAKAPLLIGCDVRSMDNSTFELLSNKEVIAVNQDELGVQGRKVKKDGNLEVWAGPLSDNKVAVVLWNRGSSQANMTAHWSDIGLSPSAVVDARDLWAXSTQSSVQGQLWAQVDSHACKMYILTPK 392

[Close]

BLAST of EH059303 vs. TrEMBL
Match: Q84VQ7_HELAN (Alpha-galactosidase OS=Helianthus annuus GN=GLA PE=2 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 2.233e-39
Identity = 80/130 (61.54%), Postives = 100/130 (76.92%), Query Frame = -2

Query:  191 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTPQ 580
            F   AL KAP+ +  ++++    T ++++N EV+AVNQD+ GVQGKKVK N DLEVWAGPL++NKVAVILWNR SS A +TA WSD+GL S T+V+ RDLW H TQ SV G ISA + SHACKMYVLTP+
Sbjct:  299 FSIWALAKAPLLVGCDMRSMSKETHDILSNREVIAVNQDSLGVQGKKVKKNGDLEVWAGPLAHNKVAVILWNRGSSRAQITAYWSDIGLNSTTVVNVRDLWAHRTQRSVKGQISATVESHACKMYVLTPR 428

[Close]

BLAST of EH059303 vs. TrEMBL
Match: Q9SP05_SOLLC (Alpha-galactosidase OS=Solanum lycopersicum GN=LeaGal PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 1.448e-38
Identity = 78/130 (60.00%), Postives = 98/130 (75.38%), Query Frame = -2

Query:  191 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTPQ 580
            F   AL KAP+ I  ++++ DNT  ++++NPEV+AVNQD  GVQGKKVK   DLEVWAGPLS  +VAV+LWNR S  A +TA WSD+GL+  T+VDARDLW HST+ SV G +SA + SH C MYVLTP+
Sbjct:  279 FSIWALVKAPLIIGCDLRSMDNTAHDILSNPEVIAVNQDKLGVQGKKVKQYGDLEVWAGPLSGKRVAVVLWNRGSYKADITAYWSDIGLDYSTLVDARDLWAHSTKGSVKGQLSASVESHDCSMYVLTPK 408

[Close]

BLAST of EH059303 vs. TrEMBL
Match: D7TXW5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_71.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034333001 PE=4 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 1.601e-37
Identity = 76/129 (58.91%), Postives = 94/129 (72.87%), Query Frame = -2

Query:  194 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLTP 580
            F   AL KAP+ I  +I++ DN T EL++N EV+ VNQD  G QGKKVK   DLEVW+GPLS+N+VAV+LWNR SS AT+ A WSD+GL S  +VDARDLW HST  S+   + A + +HACKMY LTP
Sbjct:  277 FSIWALAKAPLLIGCDIRSMDNETFELLSNKEVIEVNQDKLGAQGKKVKKTGDLEVWSGPLSDNRVAVVLWNRESSEATIIADWSDIGLNSSAVVDARDLWTHSTIYSIRHQLKATVEAHACKMYALTP 405

[Close]

BLAST of EH059303 vs. TAIR peptide
Match: AT5G08370.2 (| Symbols: AtAGAL2, AGAL2 | alpha-galactosidase 2 | chr5:2691116-2693441 REVERSE LENGTH=370)

HSP 1 Score: 154.066 bits (388), Expect = 6.185e-38
Identity = 74/128 (57.81%), Postives = 94/128 (73.44%), Query Frame = -2

Query:  197 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLT 580
            F   AL KAP+ I  ++++ D  T EL++N EV+AVNQD  G+QGKKVK   DLEVWAGPLS  +VAVILWNR S++A +TA W+++GL S  IV+ARDLW+HST S V   +SA +  HACKMY LT
Sbjct:  239 FSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKMYTLT 366

[Close]

BLAST of EH059303 vs. TAIR peptide
Match: AT5G08370.1 (| Symbols: AtAGAL2, AGAL2 | alpha-galactosidase 2 | chr5:2691116-2693441 REVERSE LENGTH=396)

HSP 1 Score: 154.066 bits (388), Expect = 6.185e-38
Identity = 74/128 (57.81%), Postives = 94/128 (73.44%), Query Frame = -2

Query:  197 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHSTQSSVSGDISAELASHACKMYVLT 580
            F   AL KAP+ I  ++++ D  T EL++N EV+AVNQD  G+QGKKVK   DLEVWAGPLS  +VAVILWNR S++A +TA W+++GL S  IV+ARDLW+HST S V   +SA +  HACKMY LT
Sbjct:  265 FSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKMYTLT 392

[Close]

BLAST of EH059303 vs. TAIR peptide
Match: AT5G08380.1 (| Symbols: AtAGAL1, AGAL1 | alpha-galactosidase 1 | chr5:2694851-2697616 REVERSE LENGTH=410)

HSP 1 Score: 131.339 bits (329), Expect = 4.293e-31
Identity = 64/135 (47.41%), Postives = 92/135 (68.15%), Query Frame = -2

Query:  194 E*IVPFQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSDLEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDHST-QSSVSGDISAELASHACKMYVLTP 595
            E IV F   A+ KAP+ +  +I+     T+E++ N EV+A+NQD  GVQ KKV+   DLEVWAGPLS  +VA++L NR  S  ++TA W D+ + + +IV+ARDLW+H T +    G+++A + SHACK+YVL P
Sbjct:  274 EYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWAGPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATVDSHACKLYVLKP 408

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BLAST of EH059303 vs. TAIR peptide
Match: AT3G56310.1 (| Symbols: | Melibiase family protein | chr3:20882886-20885745 FORWARD LENGTH=437)

HSP 1 Score: 124.02 bits (310), Expect = 6.854e-29
Identity = 62/135 (45.93%), Postives = 89/135 (65.93%), Query Frame = -2

Query:  188 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSD---LEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDH-STQSSVSGDISAELASHACKMYVLTPQS 580
            F   AL KAP+ I  +++     T+E+++N E++AVNQD  GVQG+K+++N +    +VW+GPLS +++ V LWNR S  AT+TASW  +GLES   V  RDLW H     + SG   A++ +H C MYVLTPQ+
Sbjct:  297 FSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLTPQT 431

[Close]

BLAST of EH059303 vs. TAIR peptide
Match: AT3G56310.2 (| Symbols: | Melibiase family protein | chr3:20882886-20885745 FORWARD LENGTH=413)

HSP 1 Score: 124.02 bits (310), Expect = 6.854e-29
Identity = 62/135 (45.93%), Postives = 89/135 (65.93%), Query Frame = -2

Query:  188 FQHLALGKAPV*IVLNIQAKDNTTVELITNPEVMAVNQDTRGVQGKKVKSNSD---LEVWAGPLSNNKVAVILWNRSSSNATVTASWSDLGLESGTIVDARDLWDH-STQSSVSGDISAELASHACKMYVLTPQS 580
            F   AL KAP+ I  +++     T+E+++N E++AVNQD  GVQG+K+++N +    +VW+GPLS +++ V LWNR S  AT+TASW  +GLES   V  RDLW H     + SG   A++ +H C MYVLTPQ+
Sbjct:  273 FSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLTPQT 407

The following BLAST results are available for this feature:

BLAST of EH059303 vs. SwissProt
BLAST of EH059303 vs. TrEMBL
BLAST of EH059303 vs. TAIR peptide

BLAST of EH059303 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AGAL_CYATE	1.063e-49	74.81	Alpha-galactosidase OS=Cyamopsis tetragonoloba PE=...	[more]
AGAL_COFAR	3.207e-38	60.77	Alpha-galactosidase OS=Coffea arabica PE=1 SV=1	[more]
AGAL_ORYSJ	2.810e-34	55.81	Alpha-galactosidase OS=Oryza sativa subsp. japonic...	[more]
AGAL_DICDI	5.509e-14	33.57	Probable alpha-galactosidase OS=Dictyostelium disc...	[more]
MEL_ZYGCI	4.517e-8	28.18	Alpha-galactosidase OS=Zygosaccharomyces cidri GN=...	[more]
AGALA_ASPNG	4.995e-7	31.20	Alpha-galactosidase A OS=Aspergillus niger GN=aglA...	[more]
AGALD_NEOFI	6.523e-7	27.82	Probable alpha-galactosidase D OS=Neosartorya fisc...	[more]
AGALD_ASPFU	6.523e-7	28.57	Probable alpha-galactosidase D OS=Aspergillus fumi...	[more]
AGALD_ASPFC	6.523e-7	28.57	Probable alpha-galactosidase D OS=Aspergillus fumi...	[more]
AGALA_ASPTN	6.523e-7	27.42	Probable alpha-galactosidase A OS=Aspergillus terr...	[more]

BLAST of EH059303 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q41100_PHAVU	1.444e-46	75.00	Alpha-galactosidase OS=Phaseolus vulgaris PE=2 SV=...	[more]
Q39811_SOYBN	2.464e-46	74.42	Alpha galactosidase OS=Glycine max PE=2 SV=1	[more]
C6TLN0_SOYBN	2.464e-46	74.42	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9N7Z5_POPTR	7.418e-43	66.15	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SI47_RICCO	1.827e-41	67.69	Alpha-galactosidase/alpha-n-acetylgalactosaminidas...	[more]
D7TXW6_VITVI	4.070e-41	62.31	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5ALZ9_VITVI	4.500e-40	61.54	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q84VQ7_HELAN	2.233e-39	61.54	Alpha-galactosidase OS=Helianthus annuus GN=GLA PE...	[more]
Q9SP05_SOLLC	1.448e-38	60.00	Alpha-galactosidase OS=Solanum lycopersicum GN=Lea...	[more]
D7TXW5_VITVI	1.601e-37	58.91	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of EH059303 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT5G08370.2	6.185e-38	57.81	\| Symbols: AtAGAL2, AGAL2 \| alpha-galactosidase 2 ...	[more]
AT5G08370.1	6.185e-38	57.81	\| Symbols: AtAGAL2, AGAL2 \| alpha-galactosidase 2 ...	[more]
AT5G08380.1	4.293e-31	47.41	\| Symbols: AtAGAL1, AGAL1 \| alpha-galactosidase 1 ...	[more]
AT3G56310.1	6.854e-29	45.93	\| Symbols: \| Melibiase family protein \| chr3:2088...	[more]
AT3G56310.2	6.854e-29	45.93	\| Symbols: \| Melibiase family protein \| chr3:2088...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013780	Glycosyl hydrolase, family 13, all-beta	GENE3D	2.60.40.1180	no description	coord: 30..118 score: 7.6
None	No IPR available	PANTHER	PTHR11452	ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE	coord: 9..108 score: 8.6
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 18..116 score: 2.7

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EH059303 ID=EH059303; Name=EH059303; organism=Cicer arietinum; type=EST; length=650bp
ACTTTTTTTTGGTGACCGACTTGTATATTTATTCTTACATTTCTAGATGA
AAAATAATTTTCTGTTCCTCCATTTGTCGCTCCCCTTTAGTTTAAGAGCA
GTGAATGTGTGAATGCTTTGCCAATACAGTAATTATCAAAACCTGACAAC
TACTGTTTATCTTCTTCTCTGGCACTCGAAACTAACCGCTTTGGGGAGTC
AGAACATACATCTTACAAGCATGTGAAGCTAACTCAGCAGAGATGTCTCC
TGAAACCGATGATTGTGTTGAGTGATCCCATAAATCTCTAGCATCAACTA
TAGTTCCTGATTCCAGGCCTAAGTCAGACCAGGATGCAGTCACTGTTGCA
TTTGATGAACTTCTATTCCATAAGATCACTGCTACTTTGTTATTACTGAG
AGGTCCTGCCCATACCTCCAAATCACTGTTGCTTTTCACTTTCTTTCCTT
GAACTCCACGTGTATCTTGGTTTACTGCCATAACTTCAGGGTTCGTTATC
AATTCAACTGTGGTATTATCCTTTGCTTGGATATTCAAAACAATTCATAC
AGGGGCCTTACCTAATGCCAAATGCTGAAATGGCACGATTTACTCTGTAT
CTTGCTCATTTCAACTCAAACATGTCCGGATCACCTCAGCCGGACAACTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0043169	cation binding

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR013780	Glyco_hydro_b

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