FE672911

EST Overview


Name	FE672911
Unique Name	FE672911
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	201

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FE672911 vs. SwissProt
Match: AAT1_MEDSA (Aspartate aminotransferase 1 OS=Medicago sativa GN=AAT-1 PE=2 SV=2)

HSP 1 Score: 60.8474 bits (146), Expect = 2.453e-9
Identity = 29/54 (53.70%), Postives = 38/54 (70.37%), Query Frame = -3

Query:   38 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQVA 199
            MFT T + PE VS++ KEY  Y+T   R S  GLSS+TV H+A A+HA +T+VA
Sbjct:  365 MFTFTGLNPEQVSILTKEYHIYLTSDGRISMAGLSSKTVPHLAHAIHAVVTRVA 418

[Close]

BLAST of FE672911 vs. SwissProt
Match: AAT3_ARATH (Aspartate aminotransferase, chloroplastic OS=Arabidopsis thaliana GN=ASP3 PE=1 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 9.320e-9
Identity = 29/52 (55.77%), Postives = 35/52 (67.31%), Query Frame = -3

Query:   44 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQ 199
            MFT T + P  VS M KEY  YMT   R S  GLSS+TV H+ADA+HA +T+
Sbjct:  396 MFTFTGLNPAQVSFMTKEYHIYMTSDGRISMAGLSSKTVPHLADAIHAVVTK 447

[Close]

BLAST of FE672911 vs. SwissProt
Match: AATC_DAUCA (Aspartate aminotransferase, cytoplasmic OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 6.041e-8
Identity = 28/51 (54.90%), Postives = 34/51 (66.67%), Query Frame = -3

Query:   47 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALT 199
            MFT T +  E V+ M  EY  Y+T   R S  GLSSRTV H+ADA+HAA+T
Sbjct:  352 MFTFTGLNSEQVTFMTNEYHIYLTSDGRISMAGLSSRTVPHLADAIHAAVT 402

[Close]

BLAST of FE672911 vs. SwissProt
Match: AAT2_ARATH (Aspartate aminotransferase, cytoplasmic isozyme 1 OS=Arabidopsis thaliana GN=ASP2 PE=1 SV=2)

HSP 1 Score: 56.225 bits (134), Expect = 6.041e-8
Identity = 28/53 (52.83%), Postives = 36/53 (67.92%), Query Frame = -3

Query:   41 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQV 199
            MFT T +  E V  M KE+  YMT   R S  GLSS+TV H+ADA+HAA+T++
Sbjct:  352 MFTFTGLNKEQVEFMTKEFHIYMTSDGRISMAGLSSKTVPHLADAMHAAVTRL 404

[Close]

BLAST of FE672911 vs. SwissProt
Match: AATC_ORYSJ (Aspartate aminotransferase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os01g0760600 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.030e-7
Identity = 26/53 (49.06%), Postives = 36/53 (67.92%), Query Frame = -3

Query:   41 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQV 199
            MFT T +  + V+ M +EY  YMT   R S  GLS RT+ H+ADA+HAA+T++
Sbjct:  354 MFTFTGLNSDQVAFMRQEYHIYMTSDGRISMAGLSGRTIPHLADAIHAAVTKL 406

[Close]

BLAST of FE672911 vs. SwissProt
Match: AAT4_ARATH (Aspartate aminotransferase, cytoplasmic isozyme 2 OS=Arabidopsis thaliana GN=ASP4 PE=2 SV=2)

HSP 1 Score: 53.1434 bits (126), Expect = 5.114e-7
Identity = 27/54 (50.00%), Postives = 35/54 (64.81%), Query Frame = -3

Query:   38 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQVA 199
            MFT T +  E V +M KEY  YMT   R S   LSS+TV  +ADA+HA +T++A
Sbjct:  350 MFTFTGLSEEQVRLMAKEYHIYMTYDGRISMASLSSKTVPQLADAIHAVVTRIA 403

[Close]

BLAST of FE672911 vs. TrEMBL
Match: Q93WX7_9FABA (Aspartate aminotransferase (Fragment) OS=Securigera parviflora GN=CRAAT1 PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 7.646e-9
Identity = 31/52 (59.62%), Postives = 38/52 (73.08%), Query Frame = -3

Query:   44 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQ 199
            MFT T + PE VS M KEY  Y+T   R S  GLSS+TVSH+ADA+HAA+T+
Sbjct:  288 MFTFTGLNPEQVSFMTKEYHIYLTSDGRISMAGLSSKTVSHLADAIHAAVTR 339

[Close]

BLAST of FE672911 vs. TrEMBL
Match: Q43781_LOTJA (Aspartate aminotransferase OS=Lotus japonicus GN=AAT PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.225e-8
Identity = 30/54 (55.56%), Postives = 38/54 (70.37%), Query Frame = -3

Query:   38 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQVA 199
            MFT T + PE VS M KEY  Y+T   R S  GLSS+TV H+ADA+HAA+T+ +
Sbjct:  365 MFTFTGLNPEQVSFMTKEYHIYLTSDGRISMAGLSSKTVPHLADAIHAAVTRAS 418

[Close]

BLAST of FE672911 vs. TrEMBL
Match: D7TBL7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_16.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00015367001 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 4.956e-8
Identity = 30/53 (56.60%), Postives = 37/53 (69.81%), Query Frame = -3

Query:   41 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQV 199
            MFT T +  E V+ M KEY  YMT   R S  GLSSRTV H+ADA+HAA+T++
Sbjct:  358 MFTFTGLNTEQVAFMTKEYHIYMTSDGRISMAGLSSRTVPHLADAIHAAVTRI 410

[Close]

BLAST of FE672911 vs. TrEMBL
Match: A9PHH1_POPTR (Aspartate aminotransferase OS=Populus trichocarpa GN=POPTRDRAFT_835881 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.104e-7
Identity = 30/53 (56.60%), Postives = 36/53 (67.92%), Query Frame = -3

Query:   41 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQV 199
            MFT T +  E V+ M KEY  YMT   R S  GLSSRTV H+ DA+HAA+T+V
Sbjct:  354 MFTFTGLNTEQVAFMTKEYHIYMTSDGRISMAGLSSRTVPHLTDAIHAAVTRV 406

[Close]

BLAST of FE672911 vs. TrEMBL
Match: Q40108_LUPAN (Aspartate aminotransferase OS=Lupinus angustifolius PE=3 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.883e-7
Identity = 29/53 (54.72%), Postives = 37/53 (69.81%), Query Frame = -3

Query:   41 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQV 199
            MFT T +  E VS + KEY  Y+T   R S  GLSS+TV H+ADA+HAA+T+V
Sbjct:  367 MFTFTGLNAEQVSFLTKEYHIYLTSDGRISMAGLSSKTVPHLADAIHAAVTRV 419

[Close]

BLAST of FE672911 vs. TrEMBL
Match: Q40107_LUPAN (Aspartate aminotransferase OS=Lupinus angustifolius GN=AAT-P1 PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.883e-7
Identity = 29/53 (54.72%), Postives = 37/53 (69.81%), Query Frame = -3

Query:   41 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQV 199
            MFT T +  E VS + KEY  Y+T   R S  GLSS+TV H+ADA+HAA+T+V
Sbjct:  367 MFTFTGLNAEQVSFLTKEYHIYLTSDGRISMAGLSSKTVPHLADAIHAAVTRV 419

[Close]

BLAST of FE672911 vs. TrEMBL
Match: D7M3R5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487989 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.883e-7
Identity = 29/52 (55.77%), Postives = 35/52 (67.31%), Query Frame = -3

Query:   44 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQ 199
            MFT T + P  VS M KEY  YMT   R S  GLSS+TV H+ADA+HA +T+
Sbjct:  396 MFTFTGLNPAQVSFMTKEYHIYMTSDGRISMAGLSSKTVPHLADAIHAVVTK 447

[Close]

BLAST of FE672911 vs. TrEMBL
Match: B9RKN9_RICCO (Aspartate aminotransferase OS=Ricinus communis GN=RCOM_1052360 PE=3 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.883e-7
Identity = 29/52 (55.77%), Postives = 36/52 (69.23%), Query Frame = -3

Query:   44 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQ 199
            MFT T +  E V+ M KEY  YMT   R S  GLSS+TV H+ADA+HAA+T+
Sbjct:  351 MFTFTGLNSEQVAFMTKEYHIYMTADGRISMAGLSSKTVPHLADAIHAAVTR 402

[Close]

BLAST of FE672911 vs. TrEMBL
Match: B9RS47_RICCO (Aspartate aminotransferase OS=Ricinus communis GN=RCOM_0802970 PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.460e-7
Identity = 29/52 (55.77%), Postives = 36/52 (69.23%), Query Frame = -3

Query:   44 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQ 199
            MFT T +  E V+ M KEY  YMT   R S  GLSS+TV H+ADA+HAA+T+
Sbjct:  387 MFTFTGLNSEQVAFMTKEYHIYMTSDGRISMAGLSSKTVPHLADAIHAAVTR 438

[Close]

BLAST of FE672911 vs. TrEMBL
Match: B4FUH2_MAIZE (Aspartate aminotransferase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 3.213e-7
Identity = 29/53 (54.72%), Postives = 36/53 (67.92%), Query Frame = -3

Query:   41 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQV 199
            MFT T +  E V+ M +EY  YMT   R S  GLS RTV H+ADA+HAA+TQ+
Sbjct:  406 MFTFTGLNSEQVAFMRQEYHIYMTSDGRISMAGLSMRTVPHLADAIHAAVTQL 458

[Close]

BLAST of FE672911 vs. TAIR peptide
Match: AT5G11520.1 (| Symbols: ASP3, YLS4 | aspartate aminotransferase 3 | chr5:3685257-3687721 REVERSE LENGTH=449)

HSP 1 Score: 58.9214 bits (141), Expect = 7.201e-10
Identity = 29/52 (55.77%), Postives = 35/52 (67.31%), Query Frame = -3

Query:   44 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQ 199
            MFT T + P  VS M KEY  YMT   R S  GLSS+TV H+ADA+HA +T+
Sbjct:  396 MFTFTGLNPAQVSFMTKEYHIYMTSDGRISMAGLSSKTVPHLADAIHAVVTK 447

[Close]

BLAST of FE672911 vs. TAIR peptide
Match: AT5G19550.1 (| Symbols: ASP2, AAT2 | aspartate aminotransferase 2 | chr5:6598201-6601597 FORWARD LENGTH=405)

HSP 1 Score: 56.225 bits (134), Expect = 4.667e-9
Identity = 28/53 (52.83%), Postives = 36/53 (67.92%), Query Frame = -3

Query:   41 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQV 199
            MFT T +  E V  M KE+  YMT   R S  GLSS+TV H+ADA+HAA+T++
Sbjct:  352 MFTFTGLNKEQVEFMTKEFHIYMTSDGRISMAGLSSKTVPHLADAMHAAVTRL 404

[Close]

BLAST of FE672911 vs. TAIR peptide
Match: AT1G62800.2 (| Symbols: ASP4 | aspartate aminotransferase 4 | chr1:23253934-23257416 REVERSE LENGTH=405)

HSP 1 Score: 53.1434 bits (126), Expect = 3.951e-8
Identity = 27/54 (50.00%), Postives = 35/54 (64.81%), Query Frame = -3

Query:   38 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQVA 199
            MFT T +  E V +M KEY  YMT   R S   LSS+TV  +ADA+HA +T++A
Sbjct:  352 MFTFTGLSEEQVRLMAKEYHIYMTYDGRISMASLSSKTVPQLADAIHAVVTRIA 405

[Close]

BLAST of FE672911 vs. TAIR peptide
Match: AT1G62800.1 (| Symbols: ASP4 | aspartate aminotransferase 4 | chr1:23253934-23257416 REVERSE LENGTH=403)

HSP 1 Score: 53.1434 bits (126), Expect = 3.951e-8
Identity = 27/54 (50.00%), Postives = 35/54 (64.81%), Query Frame = -3

Query:   38 MFTVTTVYPEPVSVMNKEYQFYMTCVTRSSAVGLSSRTVSHVADAVHAALTQVA 199
            MFT T +  E V +M KEY  YMT   R S   LSS+TV  +ADA+HA +T++A
Sbjct:  350 MFTFTGLSEEQVRLMAKEYHIYMTYDGRISMASLSSKTVPQLADAIHAVVTRIA 403

The following BLAST results are available for this feature:

BLAST of FE672911 vs. SwissProt
BLAST of FE672911 vs. TrEMBL
BLAST of FE672911 vs. TAIR peptide

BLAST of FE672911 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 6


Match Name	E-value	Identity	Description
AAT1_MEDSA	2.453e-9	53.70	Aspartate aminotransferase 1 OS=Medicago sativa GN...	[more]
AAT3_ARATH	9.320e-9	55.77	Aspartate aminotransferase, chloroplastic OS=Arabi...	[more]
AATC_DAUCA	6.041e-8	54.90	Aspartate aminotransferase, cytoplasmic OS=Daucus ...	[more]
AAT2_ARATH	6.041e-8	52.83	Aspartate aminotransferase, cytoplasmic isozyme 1 ...	[more]
AATC_ORYSJ	1.030e-7	49.06	Aspartate aminotransferase, cytoplasmic OS=Oryza s...	[more]
AAT4_ARATH	5.114e-7	50.00	Aspartate aminotransferase, cytoplasmic isozyme 2 ...	[more]

BLAST of FE672911 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q93WX7_9FABA	7.646e-9	59.62	Aspartate aminotransferase (Fragment) OS=Securiger...	[more]
Q43781_LOTJA	2.225e-8	55.56	Aspartate aminotransferase OS=Lotus japonicus GN=A...	[more]
D7TBL7_VITVI	4.956e-8	56.60	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9PHH1_POPTR	1.104e-7	56.60	Aspartate aminotransferase OS=Populus trichocarpa ...	[more]
Q40108_LUPAN	1.883e-7	54.72	Aspartate aminotransferase OS=Lupinus angustifoliu...	[more]
Q40107_LUPAN	1.883e-7	54.72	Aspartate aminotransferase OS=Lupinus angustifoliu...	[more]
D7M3R5_ARALY	1.883e-7	55.77	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9RKN9_RICCO	1.883e-7	55.77	Aspartate aminotransferase OS=Ricinus communis GN=...	[more]
B9RS47_RICCO	2.460e-7	55.77	Aspartate aminotransferase OS=Ricinus communis GN=...	[more]
B4FUH2_MAIZE	3.213e-7	54.72	Aspartate aminotransferase OS=Zea mays PE=2 SV=1	[more]

BLAST of FE672911 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G11520.1	7.201e-10	55.77	\| Symbols: ASP3, YLS4 \| aspartate aminotransferase...	[more]
AT5G19550.1	4.667e-9	52.83	\| Symbols: ASP2, AAT2 \| aspartate aminotransferase...	[more]
AT1G62800.2	3.951e-8	50.00	\| Symbols: ASP4 \| aspartate aminotransferase 4 \| c...	[more]
AT1G62800.1	3.951e-8	50.00	\| Symbols: ASP4 \| aspartate aminotransferase 4 \| c...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000796	Aspartate/other aminotransferase	PANTHER	PTHR11879	ASPARTATE AMINOTRANSFERASE	coord: 1..54 score: 2.4
IPR015422	Pyridoxal phosphate-dependent transferase, major region, subdomain 2	GENE3D	3.90.1150.10	no description	coord: 1..49 score: 3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE672911 ID=FE672911; Name=FE672911; organism=Cicer arietinum; type=EST; length=201bp
GACGAGTAGAGCTGATGCGCGCGGTGGTTATAATTTAAGCAACTTGGGTT
AAAGCTGCATGTACTGCATCCGCAACGTGTGACACCGTTCTAGAGCTCAA
GCCAACCGCGCTACTCCTCGTAACACATGTCATGTAGAATTGGTACTCTT
TATTCATGACGGAAACTGGCTCAGGATACACTGTTGTGACAGTAAACATT
C

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006520	amino acid metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008483	transaminase activity
GO:0003824	catalytic activity
GO:0030170	pyridoxal phosphate binding

Vocabulary: **INTERPRO**
Term	Definition
IPR000796	Asp_trans
IPR015422	PyrdxlP-dep_Trfase_small

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