FE669485

EST Overview


Name	FE669485
Unique Name	FE669485
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	543

Libraries


Library Name	Type
LIBEST_022821 Chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FE669485 vs. SwissProt
Match: APXT_ARATH (L-ascorbate peroxidase T, chloroplastic OS=Arabidopsis thaliana GN=APXT PE=2 SV=2)

HSP 1 Score: 235.728 bits (600), Expect = 1.073e-61
Identity = 117/144 (81.25%), Postives = 124/144 (86.11%), Query Frame = -2

Query:  108 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSPNAEKFVAAKYSSGKE 539
            MGL+DKEIVALSGAHTLGR+RPDRSGWGKPETKYTK GPG  GGQSWT +WLKFDNSYF         DLLVLPTDAALFEDPSFK YAEKYAED  AFFKDYAEAHAKLSNLG+KFDPPEGIVI+  P  EKFVAAKYS+GK+
Sbjct:  227 MGLDDKEIVALSGAHTLGRARPDRSGWGKPETKYTKTGPGEAGGQSWTVKWLKFDNSYFKDIKEKRDDDLLVLPTDAALFEDPSFKNYAEKYAEDVAAFFKDYAEAHAKLSNLGAKFDPPEGIVIENVP--EKFVAAKYSTGKK 368

[Close]

BLAST of FE669485 vs. SwissProt
Match: APX8_ORYSJ (Probable L-ascorbate peroxidase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=APX8 PE=2 SV=2)

HSP 1 Score: 230.72 bits (587), Expect = 3.451e-60
Identity = 110/133 (82.71%), Postives = 116/133 (87.22%), Query Frame = -2

Query:  141 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSPNAEK 539
            MGL+DKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPG PGGQSWT +WLKFDNSYF         DLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLS+LG+KFDPPEG  +D  P  E+
Sbjct:  232 MGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQSWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSDLGAKFDPPEGFSLDDEPAVEE 364

[Close]

BLAST of FE669485 vs. SwissProt
Match: APX7_ORYSJ (Probable L-ascorbate peroxidase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=APX7 PE=2 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 2.473e-58
Identity = 106/127 (83.46%), Postives = 114/127 (89.76%), Query Frame = -2

Query:  159 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDG 539
            MGL+DKEIV LSGAHTLGRSRP+RSGWGKPETKYTK+GPGAPGGQSWTA+WLKFDNSYF         DLLVLPTDAALFEDP+FKVYAEKYAEDQEAFFKDYA AHAKLSNLG+KF+PPEG  +DG
Sbjct:  233 MGLDDKEIVVLSGAHTLGRSRPERSGWGKPETKYTKNGPGAPGGQSWTAEWLKFDNSYFKEIKEKRDQDLLVLPTDAALFEDPTFKVYAEKYAEDQEAFFKDYAGAHAKLSNLGAKFNPPEGFTLDG 359

[Close]

BLAST of FE669485 vs. SwissProt
Match: APX6_ORYSJ (Probable L-ascorbate peroxidase 6, chloroplastic OS=Oryza sativa subsp. japonica GN=APX6 PE=2 SV=1)

HSP 1 Score: 217.238 bits (552), Expect = 3.949e-56
Identity = 102/126 (80.95%), Postives = 113/126 (89.68%), Query Frame = -2

Query:  162 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVID 539
            MGL+DKEIVALSGAHTLGRSRP+RSGWGKPETKYTK+GPGAPGGQSWT+QWLKFDNSYF         DLLVLPTDA LFED SFK+YAEKYA DQ+AFF+DYAEAHAKLSNLG+KFDPP+GI ++
Sbjct:  184 MGLSDKEIVALSGAHTLGRSRPERSGWGKPETKYTKNGPGAPGGQSWTSQWLKFDNSYFKDIKERRDEDLLVLPTDAVLFEDSSFKIYAEKYAADQDAFFEDYAEAHAKLSNLGAKFDPPKGISLE 309

[Close]

BLAST of FE669485 vs. SwissProt
Match: APXS_ARATH (L-ascorbate peroxidase S, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=APXS PE=1 SV=2)

HSP 1 Score: 214.927 bits (546), Expect = 1.960e-55
Identity = 102/125 (81.60%), Postives = 112/125 (89.60%), Query Frame = -2

Query:  165 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVI 539
            MGL+DK+IVALSGAHTLGRSRP+RSGWGKPETKYTK+GPGAPGGQSWT +WLKFDNSYF         DLLVLPTDAA+FED SFKVYAEKYA DQ+AFFKDYA AHAKLSNLG++F+PPEGIVI
Sbjct:  248 MGLDDKDIVALSGAHTLGRSRPERSGWGKPETKYTKEGPGAPGGQSWTPEWLKFDNSYFKEIKEKRDEDLLVLPTDAAIFEDSSFKVYAEKYAADQDAFFKDYAVAHAKLSNLGAEFNPPEGIVI 372

[Close]

BLAST of FE669485 vs. SwissProt
Match: APX5_ORYSJ (Probable L-ascorbate peroxidase 5, chloroplastic OS=Oryza sativa subsp. japonica GN=APX5 PE=2 SV=1)

HSP 1 Score: 213.772 bits (543), Expect = 4.366e-55
Identity = 99/126 (78.57%), Postives = 114/126 (90.48%), Query Frame = -2

Query:  162 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVID 539
            MGL+DKEIVALSGAHTLGR+RP+RSGWGKPETKYT++GPGAPGGQSWT++WLKFDNSYF         DLLVLPTDA LFED SFK++AEKYAEDQ+AFF+DYAEAHAKLSNLG+KFDPP+GI ++
Sbjct:  195 MGLSDKEIVALSGAHTLGRARPERSGWGKPETKYTENGPGAPGGQSWTSEWLKFDNSYFKEIKERRDEDLLVLPTDAVLFEDSSFKIHAEKYAEDQDAFFEDYAEAHAKLSNLGAKFDPPKGISLE 320

[Close]

BLAST of FE669485 vs. SwissProt
Match: APX3_ORYSJ (Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. japonica GN=APX3 PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 2.949e-27
Identity = 69/142 (48.59%), Postives = 87/142 (61.27%), Query Frame = -2

Query:  114 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSPNAEKFVAAKYSSG 539
            MGL+DK+IVALSG HTLG++RP+RSG+         DG       +WT   LKFDNSYF          LL LPTD AL EDP+F+ Y E YA+D++AFF+DYAE+H KLS LG  F PP    I  S    K +  + ++G
Sbjct:  147 MGLSDKDIVALSGGHTLGKARPERSGF---------DG-------AWTKDPLKFDNSYFIELLKENSEGLLKLPTDKALVEDPTFRRYVELYAKDEDAFFRDYAESHKKLSELG--FTPPRSAFIYKSCQKPKSLLMQTAAG 270

[Close]

BLAST of FE669485 vs. SwissProt
Match: APX3_ORYSI (Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. indica GN=APX3 PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 2.949e-27
Identity = 69/142 (48.59%), Postives = 87/142 (61.27%), Query Frame = -2

Query:  114 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSPNAEKFVAAKYSSG 539
            MGL+DK+IVALSG HTLG++RP+RSG+         DG       +WT   LKFDNSYF          LL LPTD AL EDP+F+ Y E YA+D++AFF+DYAE+H KLS LG  F PP    I  S    K +  + ++G
Sbjct:  147 MGLSDKDIVALSGGHTLGKARPERSGF---------DG-------AWTKDPLKFDNSYFIELLKENSEGLLKLPTDKALVEDPTFRRYVELYAKDEDAFFRDYAESHKKLSELG--FTPPRSAFIYKSCQKPKSLLMQTAAG 270

[Close]

BLAST of FE669485 vs. SwissProt
Match: APX4_ORYSJ (Probable L-ascorbate peroxidase 4 OS=Oryza sativa subsp. japonica GN=APX4 PE=2 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.113e-25
Identity = 62/114 (54.39%), Postives = 73/114 (64.04%), Query Frame = -2

Query:  198 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLG 539
            MGL+DK+IVALSG HTLGR+ P+RSG+            GA     WT + LKFDNSYF          LL LPTD AL EDPSF+ Y + YA D++ FFKDYAE+H KLS LG
Sbjct:  146 MGLSDKDIVALSGGHTLGRAHPERSGF-----------EGA-----WTQEPLKFDNSYFLELLKGESEGLLKLPTDKALLEDPSFRRYVDLYARDEDTFFKDYAESHKKLSELG 243

[Close]

BLAST of FE669485 vs. SwissProt
Match: APX3_ARATH (L-ascorbate peroxidase 3, peroxisomal OS=Arabidopsis thaliana GN=APX3 PE=1 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.113e-25
Identity = 63/119 (52.94%), Postives = 76/119 (63.87%), Query Frame = -2

Query:  183 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDP 539
            MGL+DK+IVALSG HTLGR+ P+RSG+         DGP       WT + LKFDNSYF          LL LPTD  L EDP F+   E YA+D++AFF+DYAE+H KLS LG  F+P
Sbjct:  146 MGLSDKDIVALSGGHTLGRAHPERSGF---------DGP-------WTQEPLKFDNSYFVELLKGESEGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHKKLSELG--FNP 246

[Close]

BLAST of FE669485 vs. TrEMBL
Match: Q8H1K8_9FABA (Stromal ascorbate peroxidase OS=Retama raetam PE=2 SV=1)

HSP 1 Score: 263.848 bits (673), Expect = 4.890e-69
Identity = 131/146 (89.73%), Postives = 133/146 (91.10%), Query Frame = -2

Query:  108 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSPNA--EKFVAAKYSSGKE 539
            MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYF         DLLVLPTDAALFEDP FKVYAEKYAEDQEAFFKDYAEAHAKLSNLG+KFDPPEGIVIDGSPNA  EKF+AAKYSS KE
Sbjct:  216 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFKDIKEKKDEDLLVLPTDAALFEDPCFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGAKFDPPEGIVIDGSPNAQGEKFLAAKYSSEKE 361

[Close]

BLAST of FE669485 vs. TrEMBL
Match: Q5QHW6_VIGUN (Chloroplast stromal ascorbate peroxidase OS=Vigna unguiculata PE=2 SV=1)

HSP 1 Score: 261.151 bits (666), Expect = 3.170e-68
Identity = 128/146 (87.67%), Postives = 132/146 (90.41%), Query Frame = -2

Query:  108 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSPN--AEKFVAAKYSSGKE 539
            MGLNDKEIVALSGAHTLGR+RPDRSGWGKPETKYTKDGPGAPGGQSWT QWLKFDNSYF         DLL+LPTDAALFEDPSFKVYAEKYAEDQE FFKDYAEAHAKLSNLG+KFDPPEGIVID SPN  AEKFVAAKYS+GKE
Sbjct:  219 MGLNDKEIVALSGAHTLGRARPDRSGWGKPETKYTKDGPGAPGGQSWTVQWLKFDNSYFKDIKEKKDEDLLILPTDAALFEDPSFKVYAEKYAEDQETFFKDYAEAHAKLSNLGAKFDPPEGIVIDESPNAGAEKFVAAKYSTGKE 364

[Close]

BLAST of FE669485 vs. TrEMBL
Match: Q5QHW7_VIGUN (Chloroplast thylakoid-bound ascorbate peroxidase OS=Vigna unguiculata PE=2 SV=1)

HSP 1 Score: 259.225 bits (661), Expect = 1.205e-67
Identity = 127/145 (87.59%), Postives = 131/145 (90.34%), Query Frame = -2

Query:  111 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSPN--AEKFVAAKYSSGK 539
            MGLNDKEIVALSGAHTLGR+RPDRSGWGKPETKYTKDGPGAPGGQSWT QWLKFDNSYF         DLL+LPTDAALFEDPSFKVYAEKYAEDQE FFKDYAEAHAKLSNLG+KFDPPEGIVID SPN  AEKFVAAKYS+GK
Sbjct:  219 MGLNDKEIVALSGAHTLGRARPDRSGWGKPETKYTKDGPGAPGGQSWTVQWLKFDNSYFKDIKEKKDEDLLILPTDAALFEDPSFKVYAEKYAEDQETFFKDYAEAHAKLSNLGAKFDPPEGIVIDESPNAGAEKFVAAKYSTGK 363

[Close]

BLAST of FE669485 vs. TrEMBL
Match: D2KLG9_SUASA (Stroma ascorbate peroxidase OS=Suaeda salsa PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.127e-65
Identity = 124/147 (84.35%), Postives = 129/147 (87.76%), Query Frame = -2

Query:  108 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSP---NAEKFVAAKYSSGKE 539
            MGLNDKEIVALSGAHTLGRSRP+RSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYF         +LLVLPTDAALFEDPSFKVYAEKYA DQEAFFKDYAEAHAKLSN G+KFDPPEG  +DGSP     EKFVAAKYSSGK+
Sbjct:  232 MGLNDKEIVALSGAHTLGRSRPERSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFKDIKERKDAELLVLPTDAALFEDPSFKVYAEKYAADQEAFFKDYAEAHAKLSNGGAKFDPPEGFSLDGSPAGATPEKFVAAKYSSGKD 378

[Close]

BLAST of FE669485 vs. TrEMBL
Match: D2KLH0_SUASA (Thylakoid ascorbate peroxidase OS=Suaeda salsa PE=2 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 1.923e-65
Identity = 124/146 (84.93%), Postives = 128/146 (87.67%), Query Frame = -2

Query:  111 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSP---NAEKFVAAKYSSGK 539
            MGLNDKEIVALSGAHTLGRSRP+RSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYF         +LLVLPTDAALFEDPSFKVYAEKYA DQEAFFKDYAEAHAKLSN G+KFDPPEG  +DGSP     EKFVAAKYSSGK
Sbjct:  232 MGLNDKEIVALSGAHTLGRSRPERSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFKDIKERKDAELLVLPTDAALFEDPSFKVYAEKYAADQEAFFKDYAEAHAKLSNGGAKFDPPEGFSLDGSPAGATPEKFVAAKYSSGK 377

[Close]

BLAST of FE669485 vs. TrEMBL
Match: Q9SBE2_MESCR (Stromal L-ascorbate peroxidase OS=Mesembryanthemum crystallinum PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 2.512e-65
Identity = 125/147 (85.03%), Postives = 128/147 (87.07%), Query Frame = -2

Query:  108 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSPNA---EKFVAAKYSSGKE 539
            MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYF         DLLVLPTDAALFEDPSFKVYAEKYA D EAFFKDYAEAHAKLSNLG+KFDP EG  +DGSP     EKFVAAKYSSGK+
Sbjct:  234 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFKDIKERRDEDLLVLPTDAALFEDPSFKVYAEKYAADPEAFFKDYAEAHAKLSNLGAKFDPAEGFSLDGSPAGAAPEKFVAAKYSSGKD 380

[Close]

BLAST of FE669485 vs. TrEMBL
Match: O81333_MESCR (Thylakoid-bound L-ascorbate peroxidase OS=Mesembryanthemum crystallinum PE=2 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 3.280e-65
Identity = 125/147 (85.03%), Postives = 128/147 (87.07%), Query Frame = -2

Query:  108 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSPNA---EKFVAAKYSSGKE 539
            MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYF         DLLVLPTDAALFEDPSFKVYAEKYA D EAFFKDYAEAHAKLSNLG+KFDP EG  +DGSP     EKFVAAKYSSGK+
Sbjct:  234 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFKDIKERRDEDLLVLPTDAALFEDPSFKVYAEKYAADPEAFFKDYAEAHAKLSNLGAKFDPAEGFSLDGSPAGAAPEKFVAAKYSSGKK 380

[Close]

BLAST of FE669485 vs. TrEMBL
Match: B9H7G1_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_209946 PE=4 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 3.627e-64
Identity = 121/143 (84.62%), Postives = 129/143 (90.21%), Query Frame = -2

Query:  111 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSPNAEKFVAAKYSSGK 539
            MGL+DKEI ALSGAHTLGRSRP+RSGWGKPETKYTK+GPGAPGGQSWTA+WLKFDNSYF         DLLVLPTDAALFEDPSFKVYAEKYAED+EAFFKDYAEAHAKLSNLG+KFDPPEGI++DG    EKFVAAKYSSGK
Sbjct:  149 MGLDDKEIAALSGAHTLGRSRPERSGWGKPETKYTKNGPGAPGGQSWTAEWLKFDNSYFKDIKERKDEDLLVLPTDAALFEDPSFKVYAEKYAEDKEAFFKDYAEAHAKLSNLGAKFDPPEGIMLDGVA-GEKFVAAKYSSGK 290

[Close]

BLAST of FE669485 vs. TrEMBL
Match: Q4JRC4_9ROSI (Stromal ascorbate peroxidase OS=Cucurbita cv. Kurokawa Amakuri PE=2 SV=1)

HSP 1 Score: 247.284 bits (630), Expect = 4.737e-64
Identity = 121/147 (82.31%), Postives = 127/147 (86.39%), Query Frame = -2

Query:  108 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVID---GSPNAEKFVAAKYSSGKE 539
            MGLND+EIVALSGAHTLGRSRP+RSGWGKPETKYTKDGPGAPGGQSWT QWLKF+NSYF         +LLVLPTDAALFEDPSFKVYAEKY EDQEAFFKDYAEAHAKLSNLG+KFDPPEGIVID     P  EKF AAKYS GK+
Sbjct:  226 MGLNDREIVALSGAHTLGRSRPERSGWGKPETKYTKDGPGAPGGQSWTVQWLKFNNSYFKDIKERRDEELLVLPTDAALFEDPSFKVYAEKYVEDQEAFFKDYAEAHAKLSNLGAKFDPPEGIVIDDASSKPAGEKFDAAKYSYGKD 372

[Close]

BLAST of FE669485 vs. TrEMBL
Match: O04873_9ROSI (Thylakoid-bound ascorbate peroxidase OS=Cucurbita cv. Kurokawa Amakuri PE=2 SV=1)

HSP 1 Score: 246.514 bits (628), Expect = 8.080e-64
Identity = 121/146 (82.88%), Postives = 126/146 (86.30%), Query Frame = -2

Query:  111 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVID---GSPNAEKFVAAKYSSGK 539
            MGLND+EIVALSGAHTLGRSRP+RSGWGKPETKYTKDGPGAPGGQSWT QWLKF+NSYF         +LLVLPTDAALFEDPSFKVYAEKY EDQEAFFKDYAEAHAKLSNLG+KFDPPEGIVID     P  EKF AAKYS GK
Sbjct:  226 MGLNDREIVALSGAHTLGRSRPERSGWGKPETKYTKDGPGAPGGQSWTVQWLKFNNSYFKDIKERRDEELLVLPTDAALFEDPSFKVYAEKYVEDQEAFFKDYAEAHAKLSNLGAKFDPPEGIVIDDASSKPAGEKFDAAKYSYGK 371

[Close]

BLAST of FE669485 vs. TAIR peptide
Match: AT1G77490.1 (| Symbols: TAPX | thylakoidal ascorbate peroxidase | chr1:29117688-29120046 FORWARD LENGTH=426)

HSP 1 Score: 235.728 bits (600), Expect = 1.166e-62
Identity = 117/144 (81.25%), Postives = 124/144 (86.11%), Query Frame = -2

Query:  108 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVIDGSPNAEKFVAAKYSSGKE 539
            MGL+DKEIVALSGAHTLGR+RPDRSGWGKPETKYTK GPG  GGQSWT +WLKFDNSYF         DLLVLPTDAALFEDPSFK YAEKYAED  AFFKDYAEAHAKLSNLG+KFDPPEGIVI+  P  EKFVAAKYS+GK+
Sbjct:  227 MGLDDKEIVALSGAHTLGRARPDRSGWGKPETKYTKTGPGEAGGQSWTVKWLKFDNSYFKDIKEKRDDDLLVLPTDAALFEDPSFKNYAEKYAEDVAAFFKDYAEAHAKLSNLGAKFDPPEGIVIENVP--EKFVAAKYSTGKK 368

[Close]

BLAST of FE669485 vs. TAIR peptide
Match: AT4G08390.3 (| Symbols: SAPX | stromal ascorbate peroxidase | chr4:5314999-5317071 FORWARD LENGTH=371)

HSP 1 Score: 214.927 bits (546), Expect = 2.129e-56
Identity = 102/125 (81.60%), Postives = 112/125 (89.60%), Query Frame = -2

Query:  165 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVI 539
            MGL+DK+IVALSGAHTLGRSRP+RSGWGKPETKYTK+GPGAPGGQSWT +WLKFDNSYF         DLLVLPTDAA+FED SFKVYAEKYA DQ+AFFKDYA AHAKLSNLG++F+PPEGIVI
Sbjct:  247 MGLDDKDIVALSGAHTLGRSRPERSGWGKPETKYTKEGPGAPGGQSWTPEWLKFDNSYFKEIKEKRDEDLLVLPTDAAIFEDSSFKVYAEKYAADQDAFFKDYAVAHAKLSNLGAEFNPPEGIVI 371

[Close]

BLAST of FE669485 vs. TAIR peptide
Match: AT4G08390.2 (| Symbols: SAPX | stromal ascorbate peroxidase | chr4:5314999-5317071 FORWARD LENGTH=372)

HSP 1 Score: 214.927 bits (546), Expect = 2.129e-56
Identity = 102/125 (81.60%), Postives = 112/125 (89.60%), Query Frame = -2

Query:  165 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVI 539
            MGL+DK+IVALSGAHTLGRSRP+RSGWGKPETKYTK+GPGAPGGQSWT +WLKFDNSYF         DLLVLPTDAA+FED SFKVYAEKYA DQ+AFFKDYA AHAKLSNLG++F+PPEGIVI
Sbjct:  248 MGLDDKDIVALSGAHTLGRSRPERSGWGKPETKYTKEGPGAPGGQSWTPEWLKFDNSYFKEIKEKRDEDLLVLPTDAAIFEDSSFKVYAEKYAADQDAFFKDYAVAHAKLSNLGAEFNPPEGIVI 372

[Close]

BLAST of FE669485 vs. TAIR peptide
Match: AT4G08390.1 (| Symbols: SAPX | stromal ascorbate peroxidase | chr4:5314999-5317071 FORWARD LENGTH=372)

HSP 1 Score: 214.927 bits (546), Expect = 2.129e-56
Identity = 102/125 (81.60%), Postives = 112/125 (89.60%), Query Frame = -2

Query:  165 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVI 539
            MGL+DK+IVALSGAHTLGRSRP+RSGWGKPETKYTK+GPGAPGGQSWT +WLKFDNSYF         DLLVLPTDAA+FED SFKVYAEKYA DQ+AFFKDYA AHAKLSNLG++F+PPEGIVI
Sbjct:  248 MGLDDKDIVALSGAHTLGRSRPERSGWGKPETKYTKEGPGAPGGQSWTPEWLKFDNSYFKEIKEKRDEDLLVLPTDAAIFEDSSFKVYAEKYAADQDAFFKDYAVAHAKLSNLGAEFNPPEGIVI 372

[Close]

BLAST of FE669485 vs. TAIR peptide
Match: AT4G08390.4 (| Symbols: SAPX | stromal ascorbate peroxidase | chr4:5314999-5317071 FORWARD LENGTH=346)

HSP 1 Score: 177.563 bits (449), Expect = 3.767e-45
Identity = 87/125 (69.60%), Postives = 96/125 (76.80%), Query Frame = -2

Query:  165 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDPPEGIVI 539
            MGL+DK+IVALSGAHTLGRSRP+RSGWGKPETKYTK+GPGAPGGQSWT +WLKFDNSY                          FKVYAEKYA DQ+AFFKDYA AHAKLSNLG++F+PPEGIVI
Sbjct:  248 MGLDDKDIVALSGAHTLGRSRPERSGWGKPETKYTKEGPGAPGGQSWTPEWLKFDNSY--------------------------FKVYAEKYAADQDAFFKDYAVAHAKLSNLGAEFNPPEGIVI 346

[Close]

BLAST of FE669485 vs. TAIR peptide
Match: AT4G35000.1 (| Symbols: APX3 | ascorbate peroxidase 3 | chr4:16665007-16667541 REVERSE LENGTH=287)

HSP 1 Score: 115.161 bits (287), Expect = 2.296e-26
Identity = 63/119 (52.94%), Postives = 76/119 (63.87%), Query Frame = -2

Query:  183 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLGSKFDP 539
            MGL+DK+IVALSG HTLGR+ P+RSG+         DGP       WT + LKFDNSYF          LL LPTD  L EDP F+   E YA+D++AFF+DYAE+H KLS LG  F+P
Sbjct:  146 MGLSDKDIVALSGGHTLGRAHPERSGF---------DGP-------WTQEPLKFDNSYFVELLKGESEGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHKKLSELG--FNP 246

[Close]

BLAST of FE669485 vs. TAIR peptide
Match: AT3G09640.2 (| Symbols: APX2, APX1B | ascorbate peroxidase 2 | chr3:2956301-2958163 FORWARD LENGTH=251)

HSP 1 Score: 106.301 bits (264), Expect = 1.067e-23
Identity = 60/114 (52.63%), Postives = 68/114 (59.65%), Query Frame = -2

Query:  198 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLG 539
            MGLNDK+IVALSG HTLGR   +RSG+            GA     WT   L FDNSYF          LL LPTD AL +DP F  + EKYA D++AFF+DY EAH KLS LG
Sbjct:  149 MGLNDKDIVALSGGHTLGRCHKERSGF-----------EGA-----WTPNPLIFDNSYFKEILSGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSELG 246

[Close]

BLAST of FE669485 vs. TAIR peptide
Match: AT3G09640.1 (| Symbols: APX2, APX1B | ascorbate peroxidase 2 | chr3:2956301-2958163 FORWARD LENGTH=251)

HSP 1 Score: 106.301 bits (264), Expect = 1.067e-23
Identity = 60/114 (52.63%), Postives = 68/114 (59.65%), Query Frame = -2

Query:  198 MGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLG 539
            MGLNDK+IVALSG HTLGR   +RSG+            GA     WT   L FDNSYF          LL LPTD AL +DP F  + EKYA D++AFF+DY EAH KLS LG
Sbjct:  149 MGLNDKDIVALSGGHTLGRCHKERSGF-----------EGA-----WTPNPLIFDNSYFKEILSGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSELG 246

[Close]

BLAST of FE669485 vs. TAIR peptide
Match: AT1G07890.8 (| Symbols: APX1, MEE6, CS1, ATAPX1, ATAPX01 | ascorbate peroxidase 1 | chr1:2438005-2439435 FORWARD LENGTH=250)

HSP 1 Score: 104.76 bits (260), Expect = 3.103e-23
Identity = 60/115 (52.17%), Postives = 70/115 (60.87%), Query Frame = -2

Query:  198 EMGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLG 542
            +MGL+DK+IVALSGAHTLGR   DRSG+            GA     WT+  L FDNSYF          LL L +D AL +DP F+   EKYA D++AFF DYAEAH KLS LG
Sbjct:  148 QMGLSDKDIVALSGAHTLGRCHKDRSGF-----------EGA-----WTSNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHMKLSELG 246

[Close]

BLAST of FE669485 vs. TAIR peptide
Match: AT1G07890.7 (| Symbols: APX1, MEE6, CS1, ATAPX1, ATAPX01 | ascorbate peroxidase 1 | chr1:2438005-2439435 FORWARD LENGTH=250)

HSP 1 Score: 104.76 bits (260), Expect = 3.103e-23
Identity = 60/115 (52.17%), Postives = 70/115 (60.87%), Query Frame = -2

Query:  198 EMGLNDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTAQWLKFDNSYFXXXXXXXXXDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSNLG 542
            +MGL+DK+IVALSGAHTLGR   DRSG+            GA     WT+  L FDNSYF          LL L +D AL +DP F+   EKYA D++AFF DYAEAH KLS LG
Sbjct:  148 QMGLSDKDIVALSGAHTLGRCHKDRSGF-----------EGA-----WTSNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHMKLSELG 246

The following BLAST results are available for this feature:

BLAST of FE669485 vs. SwissProt
BLAST of FE669485 vs. TrEMBL
BLAST of FE669485 vs. TAIR peptide

BLAST of FE669485 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
APXT_ARATH	1.073e-61	81.25	L-ascorbate peroxidase T, chloroplastic OS=Arabido...	[more]
APX8_ORYSJ	3.451e-60	82.71	Probable L-ascorbate peroxidase 8, chloroplastic O...	[more]
APX7_ORYSJ	2.473e-58	83.46	Probable L-ascorbate peroxidase 7, chloroplastic O...	[more]
APX6_ORYSJ	3.949e-56	80.95	Probable L-ascorbate peroxidase 6, chloroplastic O...	[more]
APXS_ARATH	1.960e-55	81.60	L-ascorbate peroxidase S, chloroplastic/mitochondr...	[more]
APX5_ORYSJ	4.366e-55	78.57	Probable L-ascorbate peroxidase 5, chloroplastic O...	[more]
APX3_ORYSJ	2.949e-27	48.59	Probable L-ascorbate peroxidase 3 OS=Oryza sativa ...	[more]
APX3_ORYSI	2.949e-27	48.59	Probable L-ascorbate peroxidase 3 OS=Oryza sativa ...	[more]
APX4_ORYSJ	2.113e-25	54.39	Probable L-ascorbate peroxidase 4 OS=Oryza sativa ...	[more]
APX3_ARATH	2.113e-25	52.94	L-ascorbate peroxidase 3, peroxisomal OS=Arabidops...	[more]

BLAST of FE669485 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q8H1K8_9FABA	4.890e-69	89.73	Stromal ascorbate peroxidase OS=Retama raetam PE=2...	[more]
Q5QHW6_VIGUN	3.170e-68	87.67	Chloroplast stromal ascorbate peroxidase OS=Vigna ...	[more]
Q5QHW7_VIGUN	1.205e-67	87.59	Chloroplast thylakoid-bound ascorbate peroxidase O...	[more]
D2KLG9_SUASA	1.127e-65	84.35	Stroma ascorbate peroxidase OS=Suaeda salsa PE=2 S...	[more]
D2KLH0_SUASA	1.923e-65	84.93	Thylakoid ascorbate peroxidase OS=Suaeda salsa PE=...	[more]
Q9SBE2_MESCR	2.512e-65	85.03	Stromal L-ascorbate peroxidase OS=Mesembryanthemum...	[more]
O81333_MESCR	3.280e-65	85.03	Thylakoid-bound L-ascorbate peroxidase OS=Mesembry...	[more]
B9H7G1_POPTR	3.627e-64	84.62	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
Q4JRC4_9ROSI	4.737e-64	82.31	Stromal ascorbate peroxidase OS=Cucurbita cv. Kuro...	[more]
O04873_9ROSI	8.080e-64	82.88	Thylakoid-bound ascorbate peroxidase OS=Cucurbita ...	[more]

BLAST of FE669485 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G77490.1	1.166e-62	81.25	\| Symbols: TAPX \| thylakoidal ascorbate peroxidase...	[more]
AT4G08390.3	2.129e-56	81.60	\| Symbols: SAPX \| stromal ascorbate peroxidase \| c...	[more]
AT4G08390.2	2.129e-56	81.60	\| Symbols: SAPX \| stromal ascorbate peroxidase \| c...	[more]
AT4G08390.1	2.129e-56	81.60	\| Symbols: SAPX \| stromal ascorbate peroxidase \| c...	[more]
AT4G08390.4	3.767e-45	69.60	\| Symbols: SAPX \| stromal ascorbate peroxidase \| c...	[more]
AT4G35000.1	2.296e-26	52.94	\| Symbols: APX3 \| ascorbate peroxidase 3 \| chr4:16...	[more]
AT3G09640.2	1.067e-23	52.63	\| Symbols: APX2, APX1B \| ascorbate peroxidase 2 \| ...	[more]
AT3G09640.1	1.067e-23	52.63	\| Symbols: APX2, APX1B \| ascorbate peroxidase 2 \| ...	[more]
AT1G07890.8	3.103e-23	52.17	\| Symbols: APX1, MEE6, CS1, ATAPX1, ATAPX01 \| asco...	[more]
AT1G07890.7	3.103e-23	52.17	\| Symbols: APX1, MEE6, CS1, ATAPX1, ATAPX01 \| asco...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 3..16 score
None	No IPR available	GENE3D	1.10.420.10	no description	coord: 1..107 score: 6.4
None	No IPR available	SEG	seg	seg	coord: 61..70 score
IPR002016	Haem peroxidase, plant/fungal/bacterial	PRINTS	PR00458	PEROXIDASE	coord: 8..23 score: 1e-06coord: 48..63 score: 1
IPR002016	Haem peroxidase, plant/fungal/bacterial	PFAM	PF00141	peroxidase	coord: 2..96 score: 6.3
IPR002016	Haem peroxidase, plant/fungal/bacterial	PROFILE	PS50873	PEROXIDASE_4	coord: 1..133 score: 11
IPR002207	Plant ascorbate peroxidase	PRINTS	PR00459	ASPEROXIDASE	coord: 2..23 score: 4.2e-09coord: 77..101 score: 4.2
IPR010255	Haem peroxidase	SUPERFAMILY	48113	Heme-dependent peroxidases	coord: 2..128 score: 4.1
IPR019793	Peroxidases heam-ligand binding site	PROSITE	PS00435	PEROXIDASE_1	coord: 8..18 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE669485 ID=FE669485; Name=FE669485; organism=Cicer arietinum; type=EST; length=543bp
ATTTTAAGACTTCCAAATTTACAGGCGATAATCACACGATTTGAAAATAG
CTTCTTCTTGTATTATTAAAAGATACTTGCACAAGAAAACAAGATACTTG
TTTTTTACTCCTTTCCAGAGGAGTACTTGGCTGCTACAAACTTCTCTGCA
TTTGGAGAACCATCAATCACAATTCCCTCTGGAGGATCAAATTTGGATCC
AAGGTTGCTGAGTTTGGCATGTGCTTCAGCATAATCTTTAAAGAATGCTT
CCTGATCTTCAGCATATTTCTCAGCATATACCTTGAAGGAAGGATCTTCA
AAAAGAGCAGCATCAGTTGGCAATACCAATAGATCTTCATCCTTTTTTTC
TTTGATGTCCTTGAAGTATGAGTTGTCAAACTTTAACCATTGTGCGGTCC
AGGATTGTCCACCAGGTGCTCCTGGCCCATCTTTCGTGTACTTAGTCTCA
GGTTTTCCCCAGCCACTACGGTCTGGTCTAGACCGCCCCAGTGTGTGCGC
ACCAGATAATGCAACAATTTCCTTGTCATTCAATCCCATTTCG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004601	peroxidase activity
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006979	response to oxidative stress
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR002016	Haem_peroxidase
IPR002207	Peroxidase_I
IPR010255	Haem_peroxidase_sf
IPR019793	Peroxidases_heam-ligand_BS

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