EH058699

EST Overview


Name	EH058699
Unique Name	EH058699
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	499

Libraries


Library Name	Type
LIBEST_020668 subtracted cDNA library of drought stressed chickpea	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28

Homology

[Close]

BLAST of EH058699 vs. SwissProt
Match: G3PA_CHLRE (Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic OS=Chlamydomonas reinhardtii GN=GAPA PE=1 SV=1)

HSP 1 Score: 40.817 bits (94), Expect = 3.470e-9
Identity = 30/59 (50.85%), Postives = 38/59 (64.41%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNCTSRASNLTFRLVDLLLINVKREKKGLFAE 263
            +L IVPT++GA+KAV+LVLPSL G L      R    T  +VDL+   V+ EKK  FAE
Sbjct:  240 ALNIVPTTTGAAKAVSLVLPSLKGKL-NGIALRVPTPTVSVVDLV---VQVEKK-TFAE 293

HSP 2 Score: 40.0466 bits (92), Expect = 3.470e-9
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  212 TMTTTHSYTGDQRLLDASHRDLRRAR 237

[Close]

BLAST of EH058699 vs. SwissProt
Match: G3PA_PEA (Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic OS=Pisum sativum GN=GAPA PE=2 SV=2)

HSP 1 Score: 40.0466 bits (92), Expect = 7.674e-9
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  244 TMTTTHSYTGDQRLLDASHRDLRRAR 269

HSP 2 Score: 39.6614 bits (91), Expect = 7.674e-9
Identity = 19/26 (73.08%), Postives = 23/26 (88.46%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL 164
            +L IVPTS+GA+KAVALVLP+L G L
Sbjct:  272 ALNIVPTSTGAAKAVALVLPTLKGKL 297

[Close]

BLAST of EH058699 vs. SwissProt
Match: G3PA_SCEVA (Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic (Fragment) OS=Scenedesmus vacuolatus GN=GapA PE=2 SV=1)

HSP 1 Score: 38.5058 bits (88), Expect = 6.082e-8
Identity = 29/59 (49.15%), Postives = 36/59 (61.02%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNCTSRASNLTFRLVDLLLINVKREKKGLFAE 263
            +L IVPT++GA+KAVALVLP L G L      R       +VDL+   V+ EKK  FAE
Sbjct:  198 ALNIVPTTTGAAKAVALVLPKLKGKL-NGIALRVPTPNVSVVDLV---VQVEKK-TFAE 251

HSP 2 Score: 38.1206 bits (87), Expect = 6.082e-8
Identity = 17/26 (65.38%), Postives = 21/26 (80.77%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMT TH  +GDQRL ++ HR+LRRAR
Sbjct:  170 TMTNTHSYTGDQRLLDASHRDLRRAR 195

[Close]

BLAST of EH058699 vs. SwissProt
Match: G3P2_ANAVT (Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=gap2 PE=3 SV=2)

HSP 1 Score: 38.5058 bits (88), Expect = 7.993e-8
Identity = 17/26 (65.38%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            +MTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  176 SMTTTHSYTGDQRLLDASHRDLRRAR 201

HSP 2 Score: 37.7354 bits (86), Expect = 7.993e-8
Identity = 17/26 (65.38%), Postives = 22/26 (84.62%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL 164
            ++ IVPTS+GA+KAVALV+P L G L
Sbjct:  204 AINIVPTSTGAAKAVALVIPELKGKL 229

[Close]

BLAST of EH058699 vs. SwissProt
Match: G3P2_ANASP (Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Anabaena sp. (strain PCC 7120) GN=gap2 PE=1 SV=1)

HSP 1 Score: 38.5058 bits (88), Expect = 7.993e-8
Identity = 17/26 (65.38%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            +MTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  176 SMTTTHSYTGDQRLLDASHRDLRRAR 201

HSP 2 Score: 37.7354 bits (86), Expect = 7.993e-8
Identity = 17/26 (65.38%), Postives = 22/26 (84.62%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL 164
            ++ IVPTS+GA+KAVALV+P L G L
Sbjct:  204 AINIVPTSTGAAKAVALVIPELKGKL 229

[Close]

BLAST of EH058699 vs. SwissProt
Match: G3P_CORGL (Glyceraldehyde-3-phosphate dehydrogenase OS=Corynebacterium glutamicum GN=gap PE=1 SV=2)

HSP 1 Score: 38.5058 bits (88), Expect = 3.722e-7
Identity = 28/70 (40.00%), Postives = 37/70 (52.86%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNC------TSRASNLTFR-----LVDLLLINVKREKKGLFAE 263
            ++ IVPTS+GA+KAVALVLP L G L          T  A++LTF       V+ +   +K    G F E
Sbjct:  203 AVNIVPTSTGAAKAVALVLPELKGKLDGYALRVPVITGSATDLTFNTKSEVTVESINAAIKEAAVGEFGE 272

HSP 2 Score: 35.4242 bits (80), Expect = 3.722e-7
Identity = 16/25 (64.00%), Postives = 20/25 (80.00%), Query Frame = 1

Query:    4 MTTTHF*SGDQRLFESIHRNLRRAR 78
            MTT H  +GDQRL ++ HR+LRRAR
Sbjct:  176 MTTVHAYTGDQRLHDAPHRDLRRAR 200

[Close]

BLAST of EH058699 vs. TrEMBL
Match: C0PRT6_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 42.3578 bits (98), Expect = 1.556e-8
Identity = 29/59 (49.15%), Postives = 38/59 (64.41%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNCTSRASNLTFRLVDLLLINVKREKKGLFAE 263
            +L IVPT++GA+KAV+LVLP L G L      R       +VD L++NV  EKKG+ AE
Sbjct:  297 ALNIVPTTTGAAKAVSLVLPQLKGKL-NGIALRVPTPNVSVVD-LVVNV--EKKGISAE 351

HSP 2 Score: 40.0466 bits (92), Expect = 1.556e-8
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  269 TMTTTHSYTGDQRLLDASHRDLRRAR 294

[Close]

BLAST of EH058699 vs. TrEMBL
Match: C0PQB6_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 42.3578 bits (98), Expect = 1.556e-8
Identity = 29/59 (49.15%), Postives = 38/59 (64.41%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNCTSRASNLTFRLVDLLLINVKREKKGLFAE 263
            +L IVPT++GA+KAV+LVLP L G L      R       +VD L++NV  EKKG+ AE
Sbjct:  297 ALNIVPTTTGAAKAVSLVLPQLKGKL-NGIALRVPTPNVSVVD-LVVNV--EKKGISAE 351

HSP 2 Score: 40.0466 bits (92), Expect = 1.556e-8
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  269 TMTTTHSYTGDQRLLDASHRDLRRAR 294

[Close]

BLAST of EH058699 vs. TrEMBL
Match: A9NVI4_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 42.3578 bits (98), Expect = 1.556e-8
Identity = 29/59 (49.15%), Postives = 38/59 (64.41%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNCTSRASNLTFRLVDLLLINVKREKKGLFAE 263
            +L IVPT++GA+KAV+LVLP L G L      R       +VD L++NV  EKKG+ AE
Sbjct:  297 ALNIVPTTTGAAKAVSLVLPQLKGKL-NGIALRVPTPNVSVVD-LVVNV--EKKGISAE 351

HSP 2 Score: 40.0466 bits (92), Expect = 1.556e-8
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  269 TMTTTHSYTGDQRLLDASHRDLRRAR 294

[Close]

BLAST of EH058699 vs. TrEMBL
Match: Q20FB5_CHLAT (Glyceraldehyde-3-phosphate dehydrogenase subunit B OS=Chlorokybus atmophyticus GN=gapB PE=2 SV=1)

HSP 1 Score: 42.3578 bits (98), Expect = 1.556e-8
Identity = 27/59 (45.76%), Postives = 36/59 (61.02%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNCTSRASNLTFRLVDLLLINVKREKKGLFAE 263
            +L IVPT++GA+KAVALVLP L G L      R       +VDL+   +  EKKG+ A+
Sbjct:  292 ALNIVPTTTGAAKAVALVLPQLKGKL-NGIALRVPTPNVSVVDLV---INTEKKGITAD 346

HSP 2 Score: 40.0466 bits (92), Expect = 1.556e-8
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  264 TMTTTHSYTGDQRLLDASHRDLRRAR 289

[Close]

BLAST of EH058699 vs. TrEMBL
Match: Q1WLW6_CHLIN (Chloroplast glyceraldehyde-3-phosphate dehydrogenase (Fragment) OS=Chlamydomonas incerta GN=GapA PE=2 SV=1)

HSP 1 Score: 41.9726 bits (97), Expect = 2.035e-8
Identity = 31/59 (52.54%), Postives = 38/59 (64.41%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNCTSRASNLTFRLVDLLLINVKREKKGLFAE 263
            +L IVPT++GA+KAVALVLPSL G L      R    T  +VDL+   V+ EKK  FAE
Sbjct:  235 ALNIVPTTTGAAKAVALVLPSLKGKL-NGIALRVPTPTVSVVDLV---VQVEKK-TFAE 288

HSP 2 Score: 40.0466 bits (92), Expect = 2.035e-8
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  207 TMTTTHSYTGDQRLLDASHRDLRRAR 232

[Close]

BLAST of EH058699 vs. TrEMBL
Match: Q1WLT5_CHLIN (Chloroplast glyceraldehyde-3-phosphate dehydrogenase (Fragment) OS=Chlamydomonas incerta GN=GapA PE=3 SV=1)

HSP 1 Score: 41.9726 bits (97), Expect = 2.041e-8
Identity = 31/59 (52.54%), Postives = 38/59 (64.41%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNCTSRASNLTFRLVDLLLINVKREKKGLFAE 263
            +L IVPT++GA+KAVALVLPSL G L      R    T  +VDL+   V+ EKK  FAE
Sbjct:  227 ALNIVPTTTGAAKAVALVLPSLKGKL-NGIALRVPTPTVSVVDLV---VQVEKK-TFAE 280

HSP 2 Score: 40.0466 bits (92), Expect = 2.041e-8
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  199 TMTTTHSYTGDQRLLDASHRDLRRAR 224

[Close]

BLAST of EH058699 vs. TrEMBL
Match: Q20FB4_MESVI (Glyceraldehyde-3-phosphate dehydrogenase subunit B OS=Mesostigma viride GN=gapB PE=2 SV=1)

HSP 1 Score: 41.2022 bits (95), Expect = 3.408e-8
Identity = 27/59 (45.76%), Postives = 35/59 (59.32%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNCTSRASNLTFRLVDLLLINVKREKKGLFAE 263
            +L IVPTS+GA+KAV+LVLP L G L      R       +VDL+   +   KKG+ AE
Sbjct:  252 ALNIVPTSTGAAKAVSLVLPKLKGKL-NGIALRVPTPNVSVVDLV---INTTKKGMTAE 306

HSP 2 Score: 40.0466 bits (92), Expect = 3.408e-8
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  224 TMTTTHSYTGDQRLLDASHRDLRRAR 249

[Close]

BLAST of EH058699 vs. TrEMBL
Match: Q41019_PINSY (Glyceraldehyde-phosphate dehydrogenase OS=Pinus sylvestris PE=2 SV=1)

HSP 1 Score: 41.2022 bits (95), Expect = 3.409e-8
Identity = 31/59 (52.54%), Postives = 37/59 (62.71%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNCTSRASNLTFRLVDLLLINVKREKKGLFAE 263
            +L IVPTS+GA+KAVALVLPSL G L      R       +VDL+   V+ EKK  FAE
Sbjct:  277 ALNIVPTSTGAAKAVALVLPSLKGKL-NGIALRVPTPNVSVVDLV---VQVEKK-TFAE 330

HSP 2 Score: 40.0466 bits (92), Expect = 3.409e-8
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  249 TMTTTHSYTGDQRLLDASHRDLRRAR 274

[Close]

BLAST of EH058699 vs. TrEMBL
Match: Q5TME3_PINTH (Glyceraldehyde-3-phosphate dehydrogenase (Fragment) OS=Pinus thunbergii GN=PtGAPDH PE=2 SV=1)

HSP 1 Score: 41.2022 bits (95), Expect = 3.520e-8
Identity = 31/59 (52.54%), Postives = 37/59 (62.71%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL*WNCTSRASNLTFRLVDLLLINVKREKKGLFAE 263
            +L IVPTS+GA+KAVALVLPSL G L      R       +VDL+   V+ EKK  FAE
Sbjct:  100 ALNIVPTSTGAAKAVALVLPSLKGKL-NGIALRVPTPNVSVVDLV---VQVEKK-TFAE 153

HSP 2 Score: 40.0466 bits (92), Expect = 3.520e-8
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:   72 TMTTTHSYTGDQRLLDASHRDLRRAR 97

[Close]

BLAST of EH058699 vs. TrEMBL
Match: C8CGU0_MEDSA (Putative glyceraldehyde-3-phosphate dehydrogenase OS=Medicago sativa GN=GAPDH PE=2 SV=1)

HSP 1 Score: 40.817 bits (94), Expect = 4.425e-8
Identity = 20/26 (76.92%), Postives = 23/26 (88.46%), Query Frame = 3

Query:   87 SLTIVPTSSGASKAVALVLPSLNGPL 164
            +L IVPTS+GA+KAVALVLPSL G L
Sbjct:  271 ALNIVPTSTGAAKAVALVLPSLKGKL 296

HSP 2 Score: 40.0466 bits (92), Expect = 4.425e-8
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 1

Query:    1 TMTTTHF*SGDQRLFESIHRNLRRAR 78
            TMTTTH  +GDQRL ++ HR+LRRAR
Sbjct:  243 TMTTTHSYTGDQRLLDASHRDLRRAR 268

The following BLAST results are available for this feature:

BLAST of EH058699 vs. SwissProt
BLAST of EH058699 vs. TrEMBL

BLAST of EH058699 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 6


Match Name	E-value	Identity	Description
G3PA_CHLRE	3.470e-9	50.85	Glyceraldehyde-3-phosphate dehydrogenase A, chloro...	[more]
G3PA_PEA	7.674e-9	69.23	Glyceraldehyde-3-phosphate dehydrogenase A, chloro...	[more]
G3PA_SCEVA	6.082e-8	49.15	Glyceraldehyde-3-phosphate dehydrogenase A, chloro...	[more]
G3P2_ANAVT	7.993e-8	65.38	Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Anab...	[more]
G3P2_ANASP	7.993e-8	65.38	Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Anab...	[more]
G3P_CORGL	3.722e-7	40.00	Glyceraldehyde-3-phosphate dehydrogenase OS=Coryne...	[more]

BLAST of EH058699 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C0PRT6_PICSI	1.556e-8	49.15	Putative uncharacterized protein OS=Picea sitchens...	[more]
C0PQB6_PICSI	1.556e-8	49.15	Putative uncharacterized protein OS=Picea sitchens...	[more]
A9NVI4_PICSI	1.556e-8	49.15	Putative uncharacterized protein OS=Picea sitchens...	[more]
Q20FB5_CHLAT	1.556e-8	45.76	Glyceraldehyde-3-phosphate dehydrogenase subunit B...	[more]
Q1WLW6_CHLIN	2.035e-8	52.54	Chloroplast glyceraldehyde-3-phosphate dehydrogena...	[more]
Q1WLT5_CHLIN	2.041e-8	52.54	Chloroplast glyceraldehyde-3-phosphate dehydrogena...	[more]
Q20FB4_MESVI	3.408e-8	45.76	Glyceraldehyde-3-phosphate dehydrogenase subunit B...	[more]
Q41019_PINSY	3.409e-8	52.54	Glyceraldehyde-phosphate dehydrogenase OS=Pinus sy...	[more]
Q5TME3_PINTH	3.520e-8	52.54	Glyceraldehyde-3-phosphate dehydrogenase (Fragment...	[more]
C8CGU0_MEDSA	4.425e-8	76.92	Putative glyceraldehyde-3-phosphate dehydrogenase ...	[more]

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EH058699 ID=EH058699; Name=EH058699; organism=Cicer arietinum; type=EST; length=499bp
ACCATGACTACCACTCACTTCTAATCCGGGGACCAAAGGCTATTTGAGTC
TATCCACCGCAACCTAAGGCGTGCTAGGTGCCACCATCACTCACCATAGT
TCCAACTTCATCCGGAGCATCAAAGGCAGTAGCCCTTGTCCTCCCATCAC
TCAATGGCCCGCTCTAATGGAATTGCACTTCGCGTGCCAGCAACCTAACA
TTCCGGCTGGTGGACCTTCTTCTAATAAACGTCAAGCGAGAAAAGAAAGG
CCTTTTTGCTGAGGGGAGCTGAGTTGAGAGCGTTTAGAAGAGGAGTGGAT
TCCTCTTTGAGCTTATAATGGGTATTACTTATTCTAGGTGTTAGTGAATT
AAATCCAACATTTGTGTTCCTGGTAAAAATTTGAAGGCGTGGCAGCTGAA
TTGTAGTTCGTTCATCCCAGTTTGAATTCTATCGTTATGAATTTATTGGT
CCAATGGGGAAGGAATGGATCGTGGGGTTTAATGGGTGGGTTTGGTTTA

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