FE668661

EST Overview


Name	FE668661
Unique Name	FE668661
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	653

Libraries


Library Name	Type
LIBEST_022821 Chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FE668661 vs. SwissProt
Match: SR54C_ARATH (Signal recognition particle 54 kDa protein, chloroplastic OS=Arabidopsis thaliana GN=FFC PE=1 SV=1)

HSP 1 Score: 259.225 bits (661), Expect = 1.348e-68
Identity = 134/166 (80.72%), Postives = 147/166 (88.55%), Query Frame = 2

Query:  155 RNSFAKDIWGLVQSKSVCVRRTMRPVFVRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            RN   ++IW  V+SK+V      R   VRAEMFGQLT GLE+AW+KLKGEEVLTK+NI EPMRDIRRALLEADVSLPVVRRFVQSV+DQAVG+GVIRGVKPDQQLVKIVH+ELV LMGG+VSEL FAK+GPTVILLAGLQGVGKTTVCAKLA YLKKQGKSCML+A
Sbjct:   46 RNLSTREIWSWVKSKTVVGHGRYRRSQVRAEMFGQLTGGLEAAWSKLKGEEVLTKDNIAEPMRDIRRALLEADVSLPVVRRFVQSVSDQAVGMGVIRGVKPDQQLVKIVHDELVKLMGGEVSELQFAKSGPTVILLAGLQGVGKTTVCAKLACYLKKQGKSCMLIA 211

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BLAST of FE668661 vs. SwissProt
Match: SRP54_SYNE7 (Signal sequence-binding protein OS=Synechococcus elongatus (strain PCC 7942) GN=ffh PE=3 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 2.837e-34
Identity = 71/135 (52.59%), Postives = 100/135 (74.07%), Query Frame = 2

Query:  248 MFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            MF  L   LE AW KL+G++ +++ N+ E ++++RRALLEADV+L VV+ F+  V ++AVG  VI G++PDQQ +K+V++ELV +MG     LA A   PTVIL+AGLQG GKTT  AKLA +L+K+G+S +LVA
Sbjct:    1 MFDALAERLEQAWTKLRGQDKISESNVQEALKEVRRALLEADVNLGVVKEFIAQVQEKAVGTQVISGIRPDQQFIKVVYDELVQVMGETHVPLAQAAKAPTVILMAGLQGAGKTTATAKLALHLRKEGRSTLLVA 135

[Close]

BLAST of FE668661 vs. SwissProt
Match: SRP54_SYNY3 (Signal recognition particle protein OS=Synechocystis sp. (strain PCC 6803) GN=ffh PE=3 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.078e-33
Identity = 73/135 (54.07%), Postives = 98/135 (72.59%), Query Frame = 2

Query:  248 MFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            MF  L   LE AW KL+G++ +++ NI E ++++RRALL ADV+L VV+ F++ V  +A+G  VI GV P QQ +KIV++ELVNLMG     LA A+  PTVIL+AGLQG GKTT  AKLA YL+KQ +S ++VA
Sbjct:    1 MFDALADRLEDAWKKLRGQDKISESNIKEALQEVRRALLAADVNLQVVKGFIKDVEQKALGADVISGVNPGQQFIKIVYDELVNLMGESNVPLAQAEQAPTVILMAGLQGTGKTTATAKLALYLRKQKRSALMVA 135

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BLAST of FE668661 vs. SwissProt
Match: SRP54_MYCTU (Signal recognition particle protein OS=Mycobacterium tuberculosis GN=ffh PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 9.444e-30
Identity = 71/135 (52.59%), Postives = 95/135 (70.37%), Query Frame = 2

Query:  248 MFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            MF  L+  L +A   L+G+  LT  +I    R+IR ALLEADVSLPVVR F+  + ++A G  V   + P QQ+VKIV+EEL++++GG+  ELAFAKT PTV++LAGLQG GKTT+  KLA  L+ QG + +LVA
Sbjct:    1 MFESLSDRLTAALQGLRGKGRLTDADIDATTREIRLALLEADVSLPVVRAFIHRIKERARGAEVSSALNPAQQVVKIVNEELISILGGETRELAFAKTPPTVVMLAGLQGSGKTTLAGKLAARLRGQGHTPLLVA 135

[Close]

BLAST of FE668661 vs. SwissProt
Match: SRP54_MYCBO (Signal recognition particle protein OS=Mycobacterium bovis GN=ffh PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 9.444e-30
Identity = 71/135 (52.59%), Postives = 95/135 (70.37%), Query Frame = 2

Query:  248 MFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            MF  L+  L +A   L+G+  LT  +I    R+IR ALLEADVSLPVVR F+  + ++A G  V   + P QQ+VKIV+EEL++++GG+  ELAFAKT PTV++LAGLQG GKTT+  KLA  L+ QG + +LVA
Sbjct:    1 MFESLSDRLTAALQGLRGKGRLTDADIDATTREIRLALLEADVSLPVVRAFIHRIKERARGAEVSSALNPAQQVVKIVNEELISILGGETRELAFAKTPPTVVMLAGLQGSGKTTLAGKLAARLRGQGHTPLLVA 135

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BLAST of FE668661 vs. SwissProt
Match: SRP54_MYCLE (Signal recognition particle protein OS=Mycobacterium leprae GN=ffh PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 6.121e-29
Identity = 69/135 (51.11%), Postives = 92/135 (68.15%), Query Frame = 2

Query:  248 MFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            MF  L+  L      L+G+  LT  +I    R+IR AL EADVSLPVVR FV  + ++A G  V   + P QQ+VKIV+EEL+ ++GG+  +LAFAKT PTV++LAGLQG GKTT+  KLA  L++QG + +LVA
Sbjct:    1 MFESLSNRLTGVIQGLRGKGRLTDADIEATTREIRLALFEADVSLPVVRAFVHRIKERARGTEVSAALNPAQQIVKIVNEELIGILGGETRQLAFAKTPPTVVMLAGLQGSGKTTLAGKLAVRLRRQGHTPLLVA 135

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BLAST of FE668661 vs. SwissProt
Match: SRP54_BACSU (Signal recognition particle protein OS=Bacillus subtilis GN=ffh PE=1 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.508e-27
Identity = 64/135 (47.41%), Postives = 95/135 (70.37%), Query Frame = 2

Query:  251 FGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYL-KKQGKSCMLVA 652
            F  L   L+   +K++G+  ++++++ E MR++R ALLEADV+  VV+ FV+ V+++AVG  V++ + P QQ++K+V EEL  LMGG+ S++A AK  PTVI++ GLQG GKTT   KLAN L KK  +  MLVA
Sbjct:    3 FEGLADRLQQTISKIRGKGKVSEQDVKEMMREVRLALLEADVNFKVVKDFVKKVSERAVGQDVMKSLTPGQQVIKVVQEELTELMGGEESKIAVAKRPPTVIMMVGLQGAGKTTTSGKLANLLRKKHNRKPMLVA 137

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BLAST of FE668661 vs. SwissProt
Match: SRP54_RICFE (Signal recognition particle protein OS=Rickettsia felis GN=ffh PE=3 SV=2)

HSP 1 Score: 112.079 bits (279), Expect = 2.661e-24
Identity = 62/137 (45.26%), Postives = 84/137 (61.31%), Query Frame = 2

Query:  248 MFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSE--LAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            MF  LT  L   ++KL    +LT+  I   MRDIR ALLE+DV+LPV++ F+  V  +A+G  VI+ V P Q ++KI+HEE++NL+    SE  L      P   L+ GLQG GKTT  +KLA  LK Q K  +LV+
Sbjct:    1 MFKTLTQNLTKIFDKLVSSGILTEAQIDAAMRDIRVALLESDVALPVIKDFIAEVKQKALGQEVIKSVSPGQMIIKIIHEEMINLLASSESETKLNLNSKPPVNFLMVGLQGSGKTTASSKLALRLKNQNKKVLLVS 137

[Close]

BLAST of FE668661 vs. SwissProt
Match: SRP54_ECOLI (Signal recognition particle protein OS=Escherichia coli (strain K12) GN=ffh PE=1 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 2.661e-24
Identity = 60/134 (44.78%), Postives = 83/134 (61.94%), Query Frame = 2

Query:  248 MFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLV 649
            MF  LT  L      + G   LT++N+ + +R++R ALLEADV+LPVVR F+  V ++AVG  V + + P Q+ VKIV  ELV  MG +   L  A   P V+L+AGLQG GKTT   KL  +L+++ K  +LV
Sbjct:    1 MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVALPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAVVLMAGLQGAGKTTSVGKLGKFLREKHKKKVLV 134

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BLAST of FE668661 vs. SwissProt
Match: SRP54_ECOL6 (Signal recognition particle protein OS=Escherichia coli O6 GN=ffh PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 2.661e-24
Identity = 60/134 (44.78%), Postives = 83/134 (61.94%), Query Frame = 2

Query:  248 MFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLV 649
            MF  LT  L      + G   LT++N+ + +R++R ALLEADV+LPVVR F+  V ++AVG  V + + P Q+ VKIV  ELV  MG +   L  A   P V+L+AGLQG GKTT   KL  +L+++ K  +LV
Sbjct:    1 MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVALPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAVVLMAGLQGAGKTTSVGKLGKFLREKHKKKVLV 134

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BLAST of FE668661 vs. TrEMBL
Match: A2Q2E1_MEDTR (GTP-binding signal recognition particle SRP54, G-domain OS=Medicago truncatula GN=MtrDRAFT_AC150442g28v2 PE=4 SV=1)

HSP 1 Score: 311.227 bits (796), Expect = 4.349e-83
Identity = 169/204 (82.84%), Postives = 176/204 (86.27%), Query Frame = 2

Query:   47 MEAVLASCHLSTPFSSHNRTTXXXXXXXXXXXXXXXRNSFAKDIWGLVQSKSVCVRRT--MRPVFVRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            MEAVLAS HLSTP S H+RTT               RNSFAK+IWG VQSKSV +RR   +RPV VRAEMFGQLTTGLESAWNKLKGEEVLTK+NIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGV RGVKPDQQLVKIVH+ELV LMGG+VSEL FAKTGPTVILLAGLQGVGKTTVCAKLANY KKQGKSCMLVA
Sbjct:    1 MEAVLASRHLSTPLS-HHRTTASSSSKLCSSLSY--RNSFAKEIWGFVQSKSVTMRRMDMIRPVVVRAEMFGQLTTGLESAWNKLKGEEVLTKDNIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVTRGVKPDQQLVKIVHDELVQLMGGEVSELTFAKTGPTVILLAGLQGVGKTTVCAKLANYFKKQGKSCMLVA 201

[Close]

BLAST of FE668661 vs. TrEMBL
Match: D7TJ83_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033862001 PE=4 SV=1)

HSP 1 Score: 274.633 bits (701), Expect = 4.511e-72
Identity = 151/215 (70.23%), Postives = 166/215 (77.21%), Query Frame = 2

Query:   47 MEAVLASCHLSTPFSS-------------HNRTTXXXXXXXXXXXXXXXRNSFAKDIWGLVQSKSVCVRRTMRPVFVRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            MEAV  S   S PFSS              +  T               RN F +++W LV+SKSV VRR M P  +RAEMFGQLT+GLE+AW KLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQ+V++QAVGVG+IRGVKPDQQLVKIVH+ELV LMGG+VSEL FAK+GPTVILLAGLQGVGKTTVCAKLA YLKKQGKSCMLVA
Sbjct:    1 MEAVPFSTVASRPFSSTSPSLSTPKSLSRRSTNTRCSWTASKLPLPLSSRNPFTREVWSLVKSKSVSVRRQM-PGVIRAEMFGQLTSGLEAAWTKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQAVSEQAVGVGLIRGVKPDQQLVKIVHDELVKLMGGEVSELVFAKSGPTVILLAGLQGVGKTTVCAKLAFYLKKQGKSCMLVA 214

[Close]

BLAST of FE668661 vs. TrEMBL
Match: O82532_PEA (Signal recognition particle 54 kDa subunit (Fragment) OS=Pisum sativum GN=Ffc PE=2 SV=1)

HSP 1 Score: 273.478 bits (698), Expect = 1.005e-71
Identity = 144/168 (85.71%), Postives = 153/168 (91.07%), Query Frame = 2

Query:  155 RNSFAKDIWGLVQSKSVCVRRT--MRPVFVRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            RNSFA++IWGLVQSKS+  RRT  +RPV VRAEMFGQLT+GLESAWNKLK EEVLTKENIVEPMRDIRRA   ADVSLPVVRRFVQSVTD+AVGVGV RGVKPDQQLVKIVH+ELV LMGG+VSEL FA +GPTVILLAGLQGVGKTTVCAKLA Y KKQGKSCMLVA
Sbjct:    5 RNSFAREIWGLVQSKSLTSRRTDMIRPVVVRAEMFGQLTSGLESAWNKLKREEVLTKENIVEPMRDIRRA--RADVSLPVVRRFVQSVTDKAVGVGVTRGVKPDQQLVKIVHDELVQLMGGEVSELTFANSGPTVILLAGLQGVGKTTVCAKLATYFKKQGKSCMLVA 170

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BLAST of FE668661 vs. TrEMBL
Match: B9IIU1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1103700 PE=4 SV=1)

HSP 1 Score: 270.396 bits (690), Expect = 8.508e-71
Identity = 140/167 (83.83%), Postives = 151/167 (90.42%), Query Frame = 2

Query:  155 RNSFAKDIWGLVQSKSVCV-RRTMRPVFVRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            RNSF K++W  V  KSV   RR M    V+AEMFGQLT+GLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSV+DQAVGVG++RGVKPDQQLVKIVH+ELV LMGG+VSEL FAK+ PTVILLAGLQGVGKTTVCAKLANYLKKQGKSCML+A
Sbjct:   57 RNSFTKEMWRWVTCKSVVTFRREMGGGVVKAEMFGQLTSGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVSDQAVGVGLVRGVKPDQQLVKIVHDELVKLMGGEVSELVFAKSAPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLIA 223

[Close]

BLAST of FE668661 vs. TrEMBL
Match: B9HC80_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_763079 PE=4 SV=1)

HSP 1 Score: 263.462 bits (672), Expect = 1.040e-68
Identity = 137/167 (82.04%), Postives = 148/167 (88.62%), Query Frame = 2

Query:  155 RNSFAKDIWGLVQSKSVC-VRRTMRPVFVRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            RNSF ++IW  V  KSV  + R M    ++AEMFGQLT+GLESAWNKLKGEEVLTKENI EPMRDIRRALLEADVSLPVVRRFVQSV DQAVGVG+IRGVKPDQQ VKIV +ELV LMGG+VSEL FAK+GPTVILLAGLQGVGKTT+CAKLANYLKKQGKSCMLVA
Sbjct:   57 RNSFTREIWRWVNCKSVVTLGREMGGGVIKAEMFGQLTSGLESAWNKLKGEEVLTKENIAEPMRDIRRALLEADVSLPVVRRFVQSVGDQAVGVGLIRGVKPDQQFVKIVRDELVKLMGGEVSELVFAKSGPTVILLAGLQGVGKTTICAKLANYLKKQGKSCMLVA 223

[Close]

BLAST of FE668661 vs. TrEMBL
Match: D7LWV6_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487173 PE=4 SV=1)

HSP 1 Score: 260.766 bits (665), Expect = 6.741e-68
Identity = 136/166 (81.93%), Postives = 149/166 (89.76%), Query Frame = 2

Query:  155 RNSFAKDIWGLVQSKSVCVRRTMRPVFVRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            RN   ++IW  V+SK+V   R  R  +VRAEMFGQLT GLE+AW+KLKGEEVLTKENI EPMRDIRRALLEADVSLPVVRRFVQSV+DQAVG+GVIRGVKPDQQLVKIVH+ELV LMGG+VSEL FAK+GPTVILLAGLQGVGKTTVCAKLA YLKKQGKSCML+A
Sbjct:   45 RNPSTREIWSWVKSKTVGQGRYRRS-YVRAEMFGQLTGGLEAAWSKLKGEEVLTKENIAEPMRDIRRALLEADVSLPVVRRFVQSVSDQAVGMGVIRGVKPDQQLVKIVHDELVKLMGGEVSELQFAKSGPTVILLAGLQGVGKTTVCAKLACYLKKQGKSCMLIA 209

[Close]

BLAST of FE668661 vs. TrEMBL
Match: B9SWF9_RICCO (Signal recognition particle protein, putative OS=Ricinus communis GN=RCOM_0292970 PE=4 SV=1)

HSP 1 Score: 257.299 bits (656), Expect = 7.453e-67
Identity = 136/169 (80.47%), Postives = 147/169 (86.98%), Query Frame = 2

Query:  155 RNSFAKDIWGLVQSKSVCVRRTMRPVF---VRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            RN F ++IW  V  KS  V  T+R  F   V+AEMFGQLTTGLESAW+KLKG E LTKENI EPMRD+RRALLEADVSLPVVRRFVQSVTD+A+GVG+IRGVKPDQQLVKIVH+ELV LMGG+VSEL FAK  PTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA
Sbjct:   53 RNLFTREIWRWVNCKSSVV--TLRRDFHSAVKAEMFGQLTTGLESAWSKLKGAETLTKENIAEPMRDMRRALLEADVSLPVVRRFVQSVTDEAIGVGLIRGVKPDQQLVKIVHDELVKLMGGEVSELVFAKYSPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 219

[Close]

BLAST of FE668661 vs. TrEMBL
Match: B9G9F9_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_33001 PE=4 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 8.824e-60
Identity = 119/147 (80.95%), Postives = 134/147 (91.16%), Query Frame = 2

Query:  212 RRTMRPVFVRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            RR    + VRAEMFGQLTTGLESAWNKL+G +VLTKENIVEPMRDIRRALLEADVSLPVVRRFV S++++A+G  +IRGV+P+QQLVKIVH+ELV LMGG+VS+L FAK+GPTVILLAGLQGVGKTTVCAKLA YLKK GKSCMLVA
Sbjct:   59 RRRGSGLVVRAEMFGQLTTGLESAWNKLRGVDVLTKENIVEPMRDIRRALLEADVSLPVVRRFVSSISEKALGSDLIRGVRPEQQLVKIVHDELVKLMGGEVSDLVFAKSGPTVILLAGLQGVGKTTVCAKLAFYLKKLGKSCMLVA 205

[Close]

BLAST of FE668661 vs. TrEMBL
Match: B8BJ55_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_35150 PE=4 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 8.824e-60
Identity = 119/147 (80.95%), Postives = 134/147 (91.16%), Query Frame = 2

Query:  212 RRTMRPVFVRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            RR    + VRAEMFGQLTTGLESAWNKL+G +VLTKENIVEPMRDIRRALLEADVSLPVVRRFV S++++A+G  +IRGV+P+QQLVKIVH+ELV LMGG+VS+L FAK+GPTVILLAGLQGVGKTTVCAKLA YLKK GKSCMLVA
Sbjct:   59 RRRGSGLVVRAEMFGQLTTGLESAWNKLRGVDVLTKENIVEPMRDIRRALLEADVSLPVVRRFVSSISEKALGSDLIRGVRPEQQLVKIVHDELVQLMGGEVSDLVFAKSGPTVILLAGLQGVGKTTVCAKLAFYLKKLGKSCMLVA 205

[Close]

BLAST of FE668661 vs. TrEMBL
Match: C5YS38_SORBI (Putative uncharacterized protein Sb08g003410 OS=Sorghum bicolor GN=Sb08g003410 PE=4 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 1.152e-59
Identity = 115/139 (82.73%), Postives = 131/139 (94.24%), Query Frame = 2

Query:  236 VRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            VRAEMFGQLTTGLESAWNKL+G ++LTKENI EPMRDIRRALLEADVSLPVVRRF++SV+++AVG  VIRGV+PDQQLVK+V++ELV LMGG+VS+L FAK+GPT+ILLAGLQGVGKTTVCAKLA YLKK GKSCMLVA
Sbjct:   74 VRAEMFGQLTTGLESAWNKLRGVDILTKENIAEPMRDIRRALLEADVSLPVVRRFIESVSEKAVGTDVIRGVRPDQQLVKVVNDELVQLMGGEVSDLVFAKSGPTIILLAGLQGVGKTTVCAKLAFYLKKMGKSCMLVA 212

[Close]

BLAST of FE668661 vs. TAIR peptide
Match: AT5G03940.1 (| Symbols: FFC, 54CP, CPSRP54, SRP54CP | chloroplast signal recognition particle 54 kDa subunit | chr5:1060265-1063257 REVERSE LENGTH=564)

HSP 1 Score: 259.225 bits (661), Expect = 1.376e-69
Identity = 134/166 (80.72%), Postives = 147/166 (88.55%), Query Frame = 2

Query:  155 RNSFAKDIWGLVQSKSVCVRRTMRPVFVRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLVA 652
            RN   ++IW  V+SK+V      R   VRAEMFGQLT GLE+AW+KLKGEEVLTK+NI EPMRDIRRALLEADVSLPVVRRFVQSV+DQAVG+GVIRGVKPDQQLVKIVH+ELV LMGG+VSEL FAK+GPTVILLAGLQGVGKTTVCAKLA YLKKQGKSCML+A
Sbjct:   46 RNLSTREIWSWVKSKTVVGHGRYRRSQVRAEMFGQLTGGLEAAWSKLKGEEVLTKDNIAEPMRDIRRALLEADVSLPVVRRFVQSVSDQAVGMGVIRGVKPDQQLVKIVHDELVKLMGGEVSELQFAKSGPTVILLAGLQGVGKTTVCAKLACYLKKQGKSCMLIA 211

[Close]

BLAST of FE668661 vs. TAIR peptide
Match: AT1G48900.2 (| Symbols: | Signal recognition particle, SRP54 subunit protein | chr1:18085031-18087743 REVERSE LENGTH=431)

HSP 1 Score: 62.003 bits (149), Expect = 3.225e-10
Identity = 38/138 (27.54%), Postives = 70/138 (50.72%), Query Frame = 2

Query:  236 VRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLV 649
            V AE+ G++T  ++    ++    ++ ++ + E + +I RALL++DVS P+V+    ++        +  G    + + + +  EL  ++       A  K   +V++  GLQG GKTT C K A Y +K+G    LV
Sbjct:    2 VLAELGGRITRAIQ----QMSNVTIIDEKALNECLNEITRALLQSDVSFPLVKEMQSNIKKIVNLEDLAAGHNKRRIIEQAIFSELCKMLDPGKPAFAPKKAKASVVMFVGLQGAGKTTTCTKYAYYHQKKGYKPALV 135

[Close]

BLAST of FE668661 vs. TAIR peptide
Match: AT1G48900.1 (| Symbols: | Signal recognition particle, SRP54 subunit protein | chr1:18084972-18087743 REVERSE LENGTH=495)

HSP 1 Score: 62.003 bits (149), Expect = 3.225e-10
Identity = 38/138 (27.54%), Postives = 70/138 (50.72%), Query Frame = 2

Query:  236 VRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLV 649
            V AE+ G++T  ++    ++    ++ ++ + E + +I RALL++DVS P+V+    ++        +  G    + + + +  EL  ++       A  K   +V++  GLQG GKTT C K A Y +K+G    LV
Sbjct:    2 VLAELGGRITRAIQ----QMSNVTIIDEKALNECLNEITRALLQSDVSFPLVKEMQSNIKKIVNLEDLAAGHNKRRIIEQAIFSELCKMLDPGKPAFAPKKAKASVVMFVGLQGAGKTTTCTKYAYYHQKKGYKPALV 135

[Close]

BLAST of FE668661 vs. TAIR peptide
Match: AT1G15310.1 (| Symbols: ATHSRP54A, SRP54-1 | signal recognition particle 54 kDa subunit | chr1:5269173-5271947 REVERSE LENGTH=479)

HSP 1 Score: 59.3066 bits (142), Expect = 2.091e-9
Identity = 37/138 (26.81%), Postives = 70/138 (50.72%), Query Frame = 2

Query:  236 VRAEMFGQLTTGLESAWNKLKGEEVLTKENIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVIRGVKPDQQLVKIVHEELVNLMGGDVSELAFAKTGPTVILLAGLQGVGKTTVCAKLANYLKKQGKSCMLV 649
            V AE+ G++T  ++    ++    ++ ++ + + + +I RALL++DVS  +V +   ++        +  G      + + + +EL  ++       A  K  P+V++  GLQG GKTT C K A Y +K+G    LV
Sbjct:    2 VLAELGGRITRAIQ----QMNNVTIIDEKVLNDFLNEITRALLQSDVSFGLVEKMQTNIKKIVNLDDLAAGHNKRLIIEQAIFKELCRMLDPGKPAFAPKKAKPSVVMFVGLQGAGKTTTCTKYAYYHQKKGYKAALV 135

The following BLAST results are available for this feature:

BLAST of FE668661 vs. SwissProt
BLAST of FE668661 vs. TrEMBL
BLAST of FE668661 vs. TAIR peptide

BLAST of FE668661 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SR54C_ARATH	1.348e-68	80.72	Signal recognition particle 54 kDa protein, chloro...	[more]
SRP54_SYNE7	2.837e-34	52.59	Signal sequence-binding protein OS=Synechococcus e...	[more]
SRP54_SYNY3	1.078e-33	54.07	Signal recognition particle protein OS=Synechocyst...	[more]
SRP54_MYCTU	9.444e-30	52.59	Signal recognition particle protein OS=Mycobacteri...	[more]
SRP54_MYCBO	9.444e-30	52.59	Signal recognition particle protein OS=Mycobacteri...	[more]
SRP54_MYCLE	6.121e-29	51.11	Signal recognition particle protein OS=Mycobacteri...	[more]
SRP54_BACSU	1.508e-27	47.41	Signal recognition particle protein OS=Bacillus su...	[more]
SRP54_RICFE	2.661e-24	45.26	Signal recognition particle protein OS=Rickettsia ...	[more]
SRP54_ECOLI	2.661e-24	44.78	Signal recognition particle protein OS=Escherichia...	[more]
SRP54_ECOL6	2.661e-24	44.78	Signal recognition particle protein OS=Escherichia...	[more]

BLAST of FE668661 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A2Q2E1_MEDTR	4.349e-83	82.84	GTP-binding signal recognition particle SRP54, G-d...	[more]
D7TJ83_VITVI	4.511e-72	70.23	Whole genome shotgun sequence of line PN40024, sca...	[more]
O82532_PEA	1.005e-71	85.71	Signal recognition particle 54 kDa subunit (Fragme...	[more]
B9IIU1_POPTR	8.508e-71	83.83	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9HC80_POPTR	1.040e-68	82.04	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7LWV6_ARALY	6.741e-68	81.93	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9SWF9_RICCO	7.453e-67	80.47	Signal recognition particle protein, putative OS=R...	[more]
B9G9F9_ORYSJ	8.824e-60	80.95	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8BJ55_ORYSI	8.824e-60	80.95	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C5YS38_SORBI	1.152e-59	82.73	Putative uncharacterized protein Sb08g003410 OS=So...	[more]

BLAST of FE668661 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G03940.1	1.376e-69	80.72	\| Symbols: FFC, 54CP, CPSRP54, SRP54CP \| chloropla...	[more]
AT1G48900.2	3.225e-10	27.54	\| Symbols: \| Signal recognition particle, SRP54 s...	[more]
AT1G48900.1	3.225e-10	27.54	\| Symbols: \| Signal recognition particle, SRP54 s...	[more]
AT1G15310.1	2.091e-9	26.81	\| Symbols: ATHSRP54A, SRP54-1 \| signal recognition...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 26..43 score
None	No IPR available	GENE3D	3.40.50.300	no description	coord: 170..211 score: 2.8
None	No IPR available	PANTHER	PTHR11564	GTPASE CONTAINING FAMILY OF SIGNAL RECOGNITION PARTICLE PROTEINS	coord: 150..211 score: 2.9
None	No IPR available	SEG	seg	seg	coord: 31..45 score
None	No IPR available	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 168..211 score: 2.1
None	No IPR available	SEG	seg	seg	coord: 88..102 score
IPR000897	Signal recognition particle, SRP54 subunit, GTPase	PFAM	PF00448	SRP54	coord: 176..211 score: 2.7
IPR004780	Signal recognition particle, SRP	PANTHER	PTHR11564:SF7	SIGNAL RECOGNITION PARTICLE PROTEIN	coord: 150..211 score: 2.9
IPR013822	Signal recognition particle, SRP54 subunit, helical bundle	GENE3D	1.20.120.140	no description	coord: 78..165 score: 3.2
IPR013822	Signal recognition particle, SRP54 subunit, helical bundle	PFAM	PF02881	SRP54_N	coord: 81..158 score: 1.1
IPR013822	Signal recognition particle, SRP54 subunit, helical bundle	SMART	SM00963	no description	coord: 77..162 score: 1.6
IPR013822	Signal recognition particle, SRP54 subunit, helical bundle	SUPERFAMILY	47364	Domain of the SRP/SRP receptor G-proteins	coord: 77..170 score: 4.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE668661 ID=FE668661; Name=FE668661; organism=Cicer arietinum; type=EST; length=653bp
ACTCATAGATTTTAGATAGATAGCACTAAGTACTGAGCTGGTGGCGATGG
AAGCCGTTCTAGCATCCTGCCATTTATCGACGCCTTTCTCTTCTCACAAT
CGAACCACATTCTCCTCTTCCAATTCGATCAATTTTTGCTCTTCCCTTTC
TTTCAGGAACTCCTTCGCGAAAGATATATGGGGTTTGGTACAGTCGAAGA
GCGTATGTGTTAGAAGAACAATGCGTCCAGTTTTTGTGAGGGCTGAAATG
TTCGGACAGTTAACCACTGGTCTTGAATCTGCTTGGAACAAGCTCAAAGG
AGAAGAGGTTTTGACCAAAGAGAATATTGTGGAGCCTATGCGAGATATCA
GGAGAGCTCTTTTAGAAGCTGATGTAAGTCTTCCTGTTGTAAGAAGGTTT
GTGCAATCTGTTACTGATCAGGCTGTGGGTGTTGGAGTCATTAGAGGCGT
CAAACCTGATCAGCAATTGGTTAAGATTGTTCACGAAGAACTTGTAAATC
TAATGGGTGGAGATGTCTCTGAATTGGCTTTTGCCAAAACTGGGCCCACT
GTCATTTTGTTGGCTGGACTCCAGGGAGTTGGGAAGACAACTGTGTGTGC
AAAGTTAGCAAATTATCTCAAGAAACAGGGAAAGAGTTGCATGCTAGTTG
CCG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005525	GTP binding
GO:0008312	7S RNA binding
GO:0005515	protein binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006614	SRP-dependent cotranslational protein targeting to membrane

Vocabulary: **Cellular Component**
Term	Definition
GO:0048500	signal recognition particle

Vocabulary: **INTERPRO**
Term	Definition
IPR000897	SRP54_GTPase_dom
IPR004780	SRP
IPR013822	Signal_recog_particl_SRP54_hlx

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