FL512444

EST Overview


Name	FL512444
Unique Name	FL512444
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	364

Libraries


Library Name	Type
LIBEST_023567 Subtracted cDNA library of drought-stressed chickpea cultivar	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FL512444 vs. SwissProt
Match: POT2_ARATH (Potassium transporter 2 OS=Arabidopsis thaliana GN=POT2 PE=1 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 1.796e-26
Identity = 48/89 (53.93%), Postives = 66/89 (74.16%), Query Frame = 2

Query:    2 SMTDVIEMEQM--DVTQRRVRFAIDD----DHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            SM DVI+M +     T RRVRFA+++    D  S + +E   +++ EL DL AAQEAG AFILGHS+V+AKQGSS++++LA+N+GYNFL
Sbjct:  679 SMEDVIQMAEPAPTATIRRVRFAVEENSYEDEGSTSSAEADAELRSELRDLLAAQEAGTAFILGHSHVKAKQGSSVMKRLAVNFGYNFL 767

HSP 2 Score: 47.3654 bits (111), Expect = 1.796e-26
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVALKVPPVSLLEVGMVY+
Sbjct:  770 NCRGPDVALKVPPVSLLEVGMVYV 793

[Close]

BLAST of FL512444 vs. SwissProt
Match: HAK8_ORYSJ (Putative potassium transporter 8 OS=Oryza sativa subsp. japonica GN=HAK8 PE=2 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 2.184e-19
Identity = 37/68 (54.41%), Postives = 50/68 (73.53%), Query Frame = 2

Query:   47 RRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            ++VRF ID  H +   +    Q+ EELE L AA++AG AFILGHS+V+ K GSSLL++LA++ GYNFL
Sbjct:  700 KQVRFFIDS-HVASPEAADNKQVAEELEALAAARDAGTAFILGHSHVQCKPGSSLLKRLAVDVGYNFL 766

HSP 2 Score: 44.669 bits (104), Expect = 2.184e-19
Identity = 19/24 (79.17%), Postives = 22/24 (91.67%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVAL+VPP SLLEVGMVY+
Sbjct:  769 NCRGPDVALRVPPASLLEVGMVYV 792

[Close]

BLAST of FL512444 vs. SwissProt
Match: HAK9_ORYSJ (Probable potassium transporter 9 OS=Oryza sativa subsp. japonica GN=HAK9 PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.893e-17
Identity = 35/68 (51.47%), Postives = 47/68 (69.12%), Query Frame = 2

Query:   47 RRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            ++VRF ++         EV   + EEL++L  A+EAG AFILGHS+V+ K GSSLL+KLA+  GYNFL
Sbjct:  699 KKVRFVVEA-----ASPEVEKGVVEELQELCEAREAGTAFILGHSHVQTKPGSSLLKKLAVGVGYNFL 761

HSP 2 Score: 43.1282 bits (100), Expect = 1.893e-17
Identity = 18/24 (75.00%), Postives = 21/24 (87.50%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DV L+VPP SLLEVGMVY+
Sbjct:  764 NCRGPDVVLRVPPASLLEVGMVYV 787

[Close]

BLAST of FL512444 vs. SwissProt
Match: POT4_ARATH (Potassium transporter 4 OS=Arabidopsis thaliana GN=POT4 PE=1 SV=2)

HSP 1 Score: 65.4698 bits (158), Expect = 2.116e-15
Identity = 33/77 (42.86%), Postives = 53/77 (68.83%), Query Frame = 2

Query:   20 EMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            E  Q  V +R VRF +     + +   +G  ++EEL DL  A+EAG+A+I+GHSYV++++ SS L+K+A++ GY+FL
Sbjct:  691 EYPQGQVRRRHVRFQL-----TASSGGMGSSVREELMDLIRAKEAGVAYIMGHSYVKSRKSSSWLKKMAIDIGYSFL 762

HSP 2 Score: 35.8094 bits (81), Expect = 2.116e-15
Identity = 14/23 (60.87%), Postives = 19/23 (82.61%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVY 321
            +CRG  VAL +P +SL+EVGM+Y
Sbjct:  765 NCRGPAVALNIPHISLIEVGMIY 787

[Close]

BLAST of FL512444 vs. SwissProt
Match: POT8_ARATH (Potassium transporter 8 OS=Arabidopsis thaliana GN=POT8 PE=2 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 3.574e-15
Identity = 38/83 (45.78%), Postives = 57/83 (68.67%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            S ++VI +      ++RVRF + +    D  +E      EEL +L  A+EAG+AFI+GHSYVRAK GSS+++K+A+N+GY+FL
Sbjct:  678 SSSEVIMVAPSPRFKKRVRFVLPESARIDRSAE------EELTELTEAREAGMAFIMGHSYVRAKSGSSVMKKIAINFGYDFL 754

HSP 2 Score: 26.5646 bits (57), Expect = 3.574e-15
Identity = 13/22 (59.09%), Postives = 13/22 (59.09%), Query Frame = 1

Query:  259 RGTDVALKVPPVSLLEVGMVYI 324
            RG    L  P  S LEVGMVYI
Sbjct:  759 RGPCYGLSTPHASTLEVGMVYI 780

[Close]

BLAST of FL512444 vs. SwissProt
Match: HAK7_ORYSJ (Potassium transporter 7 OS=Oryza sativa subsp. japonica GN=HAK7 PE=2 SV=3)

HSP 1 Score: 65.0846 bits (157), Expect = 4.639e-15
Identity = 33/84 (39.29%), Postives = 55/84 (65.48%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRR-VRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            S+  + E E   +++RR VRF I ++   D       Q+++EL DL  A+EAG+ +I+GHSYV+A++ S+ L+  A++Y Y+FL
Sbjct:  707 SLQSIYEQESGSLSRRRRVRFEIAEEERIDP------QVRDELADLLDAKEAGVTYIIGHSYVKARKNSNFLKTFAIDYAYSFL 784

HSP 2 Score: 35.039 bits (79), Expect = 4.639e-15
Identity = 14/23 (60.87%), Postives = 19/23 (82.61%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVY 321
            +CRG  VAL +P +SL+EVGM+Y
Sbjct:  787 NCRGPAVALHIPHISLVEVGMIY 809

[Close]

BLAST of FL512444 vs. SwissProt
Match: HAK10_ORYSJ (Potassium transporter 10 OS=Oryza sativa subsp. japonica GN=HAK10 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 2.343e-13
Identity = 35/69 (50.72%), Postives = 50/69 (72.46%), Query Frame = 2

Query:   44 QRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            ++RVRF +     +  R + GV+  EEL++L  A+EAG+AFILGHSYV+AK GSS  R+L +N+ Y+FL
Sbjct:  753 RKRVRFVLP---AASPRPDAGVR--EELQELMDAREAGMAFILGHSYVKAKSGSSFFRRLVINFCYDFL 816

HSP 2 Score: 27.335 bits (59), Expect = 2.343e-13
Identity = 11/21 (52.38%), Postives = 15/21 (71.43%), Query Frame = 1

Query:  259 RGTDVALKVPPVSLLEVGMVY 321
            RG + A+ +P  S LEVGM+Y
Sbjct:  821 RGPNYAVTIPHASTLEVGMIY 841

[Close]

BLAST of FL512444 vs. SwissProt
Match: POT1_ARATH (Potassium transporter 1 OS=Arabidopsis thaliana GN=POT1 PE=1 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 2.352e-13
Identity = 24/45 (53.33%), Postives = 36/45 (80.00%), Query Frame = 2

Query:  116 QEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            +EE  ++  A+EAG+A+ILGHSY +AKQ SS+L+KLA+N  + F+
Sbjct:  641 KEECMEIMEAKEAGVAYILGHSYAKAKQSSSVLKKLAVNVVFAFM 685

HSP 2 Score: 40.0466 bits (92), Expect = 2.352e-13
Identity = 18/23 (78.26%), Postives = 19/23 (82.61%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVY 321
            +CRGTDV L VP  SLLEVGMVY
Sbjct:  688 NCRGTDVVLNVPHTSLLEVGMVY 710

[Close]

BLAST of FL512444 vs. SwissProt
Match: HAK3_ORYSJ (Probable potassium transporter 3 OS=Oryza sativa subsp. japonica GN=HAK3 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.135e-13
Identity = 30/70 (42.86%), Postives = 48/70 (68.57%), Query Frame = 2

Query:   41 TQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            ++RRVRF + + H +D R      ++EEL  L  A+ AG+A+I+GHSY++A++ SS+ +K A+N  Y FL
Sbjct:  719 SRRRVRFEVANQH-TDPR------VKEELSALVEAKHAGVAYIMGHSYIKARKSSSVFKKFAVNVAYAFL 781

HSP 2 Score: 33.4982 bits (75), Expect = 5.135e-13
Identity = 12/23 (52.17%), Postives = 18/23 (78.26%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVY 321
            +CRG  + L +P +SL+EVGM+Y
Sbjct:  784 NCRGPGLVLNIPHISLIEVGMIY 806

[Close]

BLAST of FL512444 vs. SwissProt
Match: HAK2_ORYSJ (Probable potassium transporter 2 OS=Oryza sativa subsp. japonica GN=HAK2 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 9.073e-12
Identity = 27/84 (32.14%), Postives = 52/84 (61.90%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRR-VRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            S+    E E  +V++RR VRF + ++ + D       Q+++EL  L  A+  G+ +++GH Y++A++ SS  ++ A++ GY+FL
Sbjct:  679 SLQSSYEQESPNVSRRRRVRFELPEEDDMDQ------QVKDELLALVEAKHTGVTYVMGHVYIKARKNSSFFKRFAIDVGYSFL 756

HSP 2 Score: 33.4982 bits (75), Expect = 9.073e-12
Identity = 13/23 (56.52%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVY 321
            +CRG  V L +P +SL+EVGM Y
Sbjct:  759 NCRGPSVTLHIPHISLIEVGMAY 781

[Close]

BLAST of FL512444 vs. TrEMBL
Match: A5ACL8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_043599 PE=4 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 3.612e-36
Identity = 62/83 (74.70%), Postives = 76/83 (91.57%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            S+TDVIEME + VT+RRVRFAIDD+ E+DTRSE  VQ+QEELE+L+AAQ++G AFILGHS+VRAKQGSSL+R+LA+N GYNFL
Sbjct:  685 SVTDVIEMEPISVTKRRVRFAIDDESETDTRSETDVQLQEELEELWAAQQSGTAFILGHSHVRAKQGSSLVRRLAINVGYNFL 767

HSP 2 Score: 47.7506 bits (112), Expect = 3.612e-36
Identity = 22/24 (91.67%), Postives = 23/24 (95.83%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVALKVPPVSLLEVGMVYI
Sbjct:  770 NCRGPDVALKVPPVSLLEVGMVYI 793

[Close]

BLAST of FL512444 vs. TrEMBL
Match: Q06XL8_VITVI (KUP2 OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 2.299e-35
Identity = 62/83 (74.70%), Postives = 76/83 (91.57%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            S+TDVIEME + VT+RRVRFAIDD+ E+DTRSE  VQ+QEELE+L+AAQ++G AFILGHS+VRAKQGSSL+R+LA+N GYNFL
Sbjct:  684 SVTDVIEMEPISVTKRRVRFAIDDESETDTRSETDVQLQEELEELWAAQQSGTAFILGHSHVRAKQGSSLVRRLAINVGYNFL 766

HSP 2 Score: 45.0542 bits (105), Expect = 2.299e-35
Identity = 21/24 (87.50%), Postives = 22/24 (91.67%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVALKVPPVSL EVGMVYI
Sbjct:  769 NCRGPDVALKVPPVSLHEVGMVYI 792

[Close]

BLAST of FL512444 vs. TrEMBL
Match: D7T4E8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_67.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032701001 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 2.995e-35
Identity = 60/83 (72.29%), Postives = 75/83 (90.36%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            + +DVIEME + VT+RRVRFAIDD+ E+DTRSE  VQ+QEELE+L+AAQ++G AFILGHS+VRAKQGSSL+R+LA+N GYNFL
Sbjct:  657 AFSDVIEMEPISVTKRRVRFAIDDESETDTRSETDVQLQEELEELWAAQQSGTAFILGHSHVRAKQGSSLVRRLAINVGYNFL 739

HSP 2 Score: 47.7506 bits (112), Expect = 2.995e-35
Identity = 22/24 (91.67%), Postives = 23/24 (95.83%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVALKVPPVSLLEVGMVYI
Sbjct:  742 NCRGPDVALKVPPVSLLEVGMVYI 765

[Close]

BLAST of FL512444 vs. TrEMBL
Match: B9N126_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_596946 PE=4 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 2.216e-32
Identity = 56/83 (67.47%), Postives = 73/83 (87.95%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            S+TDVIEME + V +RRVRFAIDD+  S + +++ +Q+QEELEDL +AQ+AG AFILGHS+V+AKQGSSLL++LALN+GYNFL
Sbjct:  683 SVTDVIEMEPVGVVERRVRFAIDDESGSHSPADMHLQLQEELEDLLSAQQAGTAFILGHSHVKAKQGSSLLKRLALNFGYNFL 765

HSP 2 Score: 47.3654 bits (111), Expect = 2.216e-32
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVALKVPPVSLLEVGMVY+
Sbjct:  768 NCRGPDVALKVPPVSLLEVGMVYV 791

[Close]

BLAST of FL512444 vs. TrEMBL
Match: B9S4D1_RICCO (Potassium transporter, putative OS=Ricinus communis GN=RCOM_0689490 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.758e-32
Identity = 55/83 (66.27%), Postives = 71/83 (85.54%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            SM DVIEME + V +RRVRFAIDD+  +  +SE+ +Q++EELEDL+ AQ+AG AFILGHS+V+AKQGSSLL++LA+N GYNFL
Sbjct:  683 SMADVIEMEPITVVERRVRFAIDDESGTHPQSEMDLQLKEELEDLFTAQQAGTAFILGHSHVKAKQGSSLLKRLAINIGYNFL 765

HSP 2 Score: 47.7506 bits (112), Expect = 3.758e-32
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVALKVPPVSLLEVGMVY+
Sbjct:  768 NCRGADVALKVPPVSLLEVGMVYV 791

[Close]

BLAST of FL512444 vs. TrEMBL
Match: B9IQ44_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_780455 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.516e-30
Identity = 53/83 (63.86%), Postives = 71/83 (85.54%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            S+TDVIEME + V +RRVRFA DD+  + + +++ +QMQ ELEDL +AQ+AG AFILGHS+V+AKQGSS+L++LALN+GYNFL
Sbjct:  683 SVTDVIEMEPVGVVERRVRFATDDESVTLSSADMDLQMQGELEDLSSAQQAGTAFILGHSHVQAKQGSSVLKRLALNFGYNFL 765

HSP 2 Score: 48.1358 bits (113), Expect = 1.516e-30
Identity = 22/24 (91.67%), Postives = 23/24 (95.83%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVALKVPPVSLLEVGMVYI
Sbjct:  768 NCRGADVALKVPPVSLLEVGMVYI 791

[Close]

BLAST of FL512444 vs. TrEMBL
Match: B9P545_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_790873 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.542e-30
Identity = 53/83 (63.86%), Postives = 71/83 (85.54%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            S+TDVIEME + V +RRVRFA DD+  + + +++ +QMQ ELEDL +AQ+AG AFILGHS+V+AKQGSS+L++LALN+GYNFL
Sbjct:  131 SVTDVIEMEPVGVVERRVRFATDDESVTLSSADMDLQMQGELEDLSSAQQAGTAFILGHSHVQAKQGSSVLKRLALNFGYNFL 213

HSP 2 Score: 48.1358 bits (113), Expect = 1.542e-30
Identity = 22/24 (91.67%), Postives = 23/24 (95.83%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVALKVPPVSLLEVGMVYI
Sbjct:  216 NCRGADVALKVPPVSLLEVGMVYI 239

[Close]

BLAST of FL512444 vs. TrEMBL
Match: D7LF53_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_483110 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.677e-25
Identity = 48/89 (53.93%), Postives = 66/89 (74.16%), Query Frame = 2

Query:    2 SMTDVIEMEQM--DVTQRRVRFAIDD----DHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            SM DVI+M +     T RRVRFA+++    D  S + +E   +++ EL DL AAQEAG AFILGHS+V+AKQGSS++++LA+N+GYNFL
Sbjct:  679 SMEDVIQMAEAAPTATIRRVRFALEENSYEDEGSSSSAEAEAELRSELRDLLAAQEAGTAFILGHSHVKAKQGSSVMKRLAVNFGYNFL 767

HSP 2 Score: 47.3654 bits (111), Expect = 3.677e-25
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVALKVPPVSLLEVGMVY+
Sbjct:  770 NCRGPDVALKVPPVSLLEVGMVYV 793

[Close]

BLAST of FL512444 vs. TrEMBL
Match: Q94KC6_MESCR (Potassium transporter HAK2p OS=Mesembryanthemum crystallinum GN=HAK2 PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 5.127e-24
Identity = 46/83 (55.42%), Postives = 64/83 (77.11%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            S+TD++EM  +   +RRVRFA DD+       EVG  M++ELEDL+ AQ++G AFI+GHS+VRAKQGSS L+++A+N GY+FL
Sbjct:  684 SVTDIMEMGPI---KRRVRFADDDEVSGGNEKEVG--MRQELEDLWDAQQSGTAFIIGHSHVRAKQGSSFLKRVAINCGYSFL 761

HSP 2 Score: 46.2098 bits (108), Expect = 5.127e-24
Identity = 20/24 (83.33%), Postives = 23/24 (95.83%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVAL+VPPVSLLEVGMVY+
Sbjct:  764 NCRGPDVALRVPPVSLLEVGMVYV 787

[Close]

BLAST of FL512444 vs. TrEMBL
Match: A2XGG4_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_11472 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 4.471e-18
Identity = 37/68 (54.41%), Postives = 50/68 (73.53%), Query Frame = 2

Query:   47 RRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            ++VRF ID  H +   +    Q+ EELE L AA++AG AFILGHS+V+ K GSSLL++LA++ GYNFL
Sbjct:  700 KQVRFFIDS-HVASPEAADSKQVAEELEALAAARDAGTAFILGHSHVQCKPGSSLLKRLAVDVGYNFL 766

HSP 2 Score: 44.669 bits (104), Expect = 4.471e-18
Identity = 19/24 (79.17%), Postives = 22/24 (91.67%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVAL+VPP SLLEVGMVY+
Sbjct:  769 NCRGPDVALRVPPASLLEVGMVYV 792

[Close]

BLAST of FL512444 vs. TAIR peptide
Match: AT2G40540.2 (| Symbols: KT2, ATKT2, SHY3, KUP2, ATKUP2, TRK2 | potassium transporter 2 | chr2:16931445-16934516 FORWARD LENGTH=794)

HSP 1 Score: 91.2781 bits (225), Expect = 1.533e-27
Identity = 48/89 (53.93%), Postives = 66/89 (74.16%), Query Frame = 2

Query:    2 SMTDVIEMEQM--DVTQRRVRFAIDD----DHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            SM DVI+M +     T RRVRFA+++    D  S + +E   +++ EL DL AAQEAG AFILGHS+V+AKQGSS++++LA+N+GYNFL
Sbjct:  679 SMEDVIQMAEPAPTATIRRVRFAVEENSYEDEGSTSSAEADAELRSELRDLLAAQEAGTAFILGHSHVKAKQGSSVMKRLAVNFGYNFL 767

HSP 2 Score: 47.3654 bits (111), Expect = 1.533e-27
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVALKVPPVSLLEVGMVY+
Sbjct:  770 NCRGPDVALKVPPVSLLEVGMVYV 793

[Close]

BLAST of FL512444 vs. TAIR peptide
Match: AT2G40540.1 (| Symbols: KT2, ATKT2, SHY3, KUP2, ATKUP2, TRK2 | potassium transporter 2 | chr2:16931445-16934516 FORWARD LENGTH=794)

HSP 1 Score: 91.2781 bits (225), Expect = 1.533e-27
Identity = 48/89 (53.93%), Postives = 66/89 (74.16%), Query Frame = 2

Query:    2 SMTDVIEMEQM--DVTQRRVRFAIDD----DHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            SM DVI+M +     T RRVRFA+++    D  S + +E   +++ EL DL AAQEAG AFILGHS+V+AKQGSS++++LA+N+GYNFL
Sbjct:  679 SMEDVIQMAEPAPTATIRRVRFAVEENSYEDEGSTSSAEADAELRSELRDLLAAQEAGTAFILGHSHVKAKQGSSVMKRLAVNFGYNFL 767

HSP 2 Score: 47.3654 bits (111), Expect = 1.533e-27
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CRG DVALKVPPVSLLEVGMVY+
Sbjct:  770 NCRGPDVALKVPPVSLLEVGMVYV 793

[Close]

BLAST of FL512444 vs. TAIR peptide
Match: AT3G02050.1 (| Symbols: KUP3, ATKUP3, ATKT4 | K+ uptake transporter 3 | chr3:350815-354135 FORWARD LENGTH=789)

HSP 1 Score: 65.4698 bits (158), Expect = 1.807e-16
Identity = 33/77 (42.86%), Postives = 53/77 (68.83%), Query Frame = 2

Query:   20 EMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            E  Q  V +R VRF +     + +   +G  ++EEL DL  A+EAG+A+I+GHSYV++++ SS L+K+A++ GY+FL
Sbjct:  691 EYPQGQVRRRHVRFQL-----TASSGGMGSSVREELMDLIRAKEAGVAYIMGHSYVKSRKSSSWLKKMAIDIGYSFL 762

HSP 2 Score: 35.8094 bits (81), Expect = 1.807e-16
Identity = 14/23 (60.87%), Postives = 19/23 (82.61%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVY 321
            +CRG  VAL +P +SL+EVGM+Y
Sbjct:  765 NCRGPAVALNIPHISLIEVGMIY 787

[Close]

BLAST of FL512444 vs. TAIR peptide
Match: AT5G14880.1 (| Symbols: | Potassium transporter family protein | chr5:4814244-4817667 FORWARD LENGTH=781)

HSP 1 Score: 73.9442 bits (180), Expect = 3.050e-16
Identity = 38/83 (45.78%), Postives = 57/83 (68.67%), Query Frame = 2

Query:    2 SMTDVIEMEQMDVTQRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            S ++VI +      ++RVRF + +    D  +E      EEL +L  A+EAG+AFI+GHSYVRAK GSS+++K+A+N+GY+FL
Sbjct:  678 SSSEVIMVAPSPRFKKRVRFVLPESARIDRSAE------EELTELTEAREAGMAFIMGHSYVRAKSGSSVMKKIAINFGYDFL 754

HSP 2 Score: 26.5646 bits (57), Expect = 3.050e-16
Identity = 13/22 (59.09%), Postives = 13/22 (59.09%), Query Frame = 1

Query:  259 RGTDVALKVPPVSLLEVGMVYI 324
            RG    L  P  S LEVGMVYI
Sbjct:  759 RGPCYGLSTPHASTLEVGMVYI 780

[Close]

BLAST of FL512444 vs. TAIR peptide
Match: AT2G30070.1 (| Symbols: ATKT1, ATKT1P, ATKUP1, KUP1, KT1 | potassium transporter 1 | chr2:12835097-12838466 FORWARD LENGTH=712)

HSP 1 Score: 54.299 bits (129), Expect = 2.008e-14
Identity = 24/45 (53.33%), Postives = 36/45 (80.00%), Query Frame = 2

Query:  116 QEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            +EE  ++  A+EAG+A+ILGHSY +AKQ SS+L+KLA+N  + F+
Sbjct:  641 KEECMEIMEAKEAGVAYILGHSYAKAKQSSSVLKKLAVNVVFAFM 685

HSP 2 Score: 40.0466 bits (92), Expect = 2.008e-14
Identity = 18/23 (78.26%), Postives = 19/23 (82.61%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVY 321
            +CRGTDV L VP  SLLEVGMVY
Sbjct:  688 NCRGTDVVLNVPHTSLLEVGMVY 710

[Close]

BLAST of FL512444 vs. TAIR peptide
Match: AT1G70300.1 (| Symbols: KUP6 | K+ uptake permease 6 | chr1:26477993-26481233 REVERSE LENGTH=782)

HSP 1 Score: 58.5362 bits (140), Expect = 1.415e-10
Identity = 27/69 (39.13%), Postives = 48/69 (69.57%), Query Frame = 2

Query:   44 QRRVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            ++RVRF + +        ++  + ++EL +L  A+E G+A+I+G++Y++AK GS LL++LA+N GY FL
Sbjct:  693 KKRVRFVVPET------PKIEKETRQELMELTEAREGGVAYIMGNAYMKAKPGSGLLKRLAINIGYEFL 755

HSP 2 Score: 22.7126 bits (47), Expect = 1.415e-10
Identity = 11/21 (52.38%), Postives = 12/21 (57.14%), Query Frame = 1

Query:  259 RGTDVALKVPPVSLLEVGMVY 321
            RG    L  P  S LEVGM+Y
Sbjct:  760 RGPRNMLTSPHASTLEVGMIY 780

[Close]

BLAST of FL512444 vs. TAIR peptide
Match: AT4G23640.1 (| Symbols: TRH1, ATKT3, KUP4 | Potassium transporter family protein | chr4:12320476-12324291 REVERSE LENGTH=775)

HSP 1 Score: 48.9062 bits (115), Expect = 8.992e-9
Identity = 21/47 (44.68%), Postives = 36/47 (76.60%), Query Frame = 2

Query:  110 QMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            Q++EEL DL  A++A +A+I+GH +V+AK+ S  +++L +N  Y+FL
Sbjct:  702 QVKEELFDLVNAKDAEVAYIVGHGHVKAKRNSVFVKQLVVNVAYSFL 748

HSP 2 Score: 26.1794 bits (56), Expect = 8.992e-9
Identity = 10/23 (43.48%), Postives = 15/23 (65.22%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVY 321
            +CR   V L +P + L++VGM Y
Sbjct:  751 NCRSPGVMLNIPHICLIKVGMNY 773

[Close]

BLAST of FL512444 vs. TAIR peptide
Match: AT5G09400.1 (| Symbols: KUP7 | K+ uptake permease 7 | chr5:2916377-2920604 FORWARD LENGTH=858)

HSP 1 Score: 49.6766 bits (117), Expect = 4.234e-8
Identity = 23/63 (36.51%), Postives = 39/63 (61.90%), Query Frame = 2

Query:   77 HESDTRSEVGVQ-----MQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            H  DT S+  V      ++ EL  ++ A+E+G+ ++LGH  +RA++ S  ++KL +NY Y FL
Sbjct:  769 HPFDTSSDSSVSEAEQSLERELSFIHKAKESGVVYLLGHGDIRARKDSWFIKKLVINYFYTFL 831

HSP 2 Score: 23.0978 bits (48), Expect = 4.234e-8
Identity = 10/24 (41.67%), Postives = 14/24 (58.33%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CR     L VP   L++VGM Y+
Sbjct:  834 NCRRGIANLSVPQSHLMQVGMTYM 857

[Close]

BLAST of FL512444 vs. TAIR peptide
Match: AT4G33530.1 (| Symbols: KUP5 | K+ uptake permease 5 | chr4:16126503-16130353 REVERSE LENGTH=855)

HSP 1 Score: 47.3654 bits (111), Expect = 7.247e-7
Identity = 23/67 (34.33%), Postives = 41/67 (61.19%), Query Frame = 2

Query:   50 RVRFAIDDDHESDTRSEVGVQMQEELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            R + +ID      +  +V   +++EL  ++ A+E+G+ ++LGH  +RA + S  L+KL +NY Y FL
Sbjct:  762 RRKASIDFGAGPSSALDVEQSLEKELSFIHKAKESGVVYLLGHGDIRATKDSWFLKKLVINYLYAFL 828

HSP 2 Score: 21.1718 bits (43), Expect = 7.247e-7
Identity = 8/16 (50.00%), Postives = 11/16 (68.75%), Query Frame = 1

Query:  277 LKVPPVSLLEVGMVYI 324
            L VP   L++VGM Y+
Sbjct:  839 LSVPHTHLMQVGMTYM 854

[Close]

BLAST of FL512444 vs. TAIR peptide
Match: AT1G60160.1 (| Symbols: | Potassium transporter family protein | chr1:22188330-22191395 REVERSE LENGTH=827)

HSP 1 Score: 44.669 bits (104), Expect = 7.257e-7
Identity = 26/65 (40.00%), Postives = 36/65 (55.38%), Query Frame = 2

Query:   74 DHESDTRSEVGVQMQE------ELEDLYAAQEAGIAFILGHSYVRAKQGSSLLRKLALNYGYNFL 250
            D E    S VG  M+E      EL  L  A ++G+ ++L H  VRAK+ S  ++KL +NY Y FL
Sbjct:  736 DSEVLPSSSVGSSMEEDPALEYELAALREATDSGLTYLLAHGDVRAKKNSIFVKKLVINYFYAFL 800

HSP 2 Score: 23.8682 bits (50), Expect = 7.257e-7
Identity = 9/24 (37.50%), Postives = 15/24 (62.50%), Query Frame = 1

Query:  253 SCRGTDVALKVPPVSLLEVGMVYI 324
            +CR     L VP +++L+ GM Y+
Sbjct:  803 NCRAGAANLTVPHMNILQAGMTYM 826

The following BLAST results are available for this feature:

BLAST of FL512444 vs. SwissProt
BLAST of FL512444 vs. TrEMBL
BLAST of FL512444 vs. TAIR peptide

BLAST of FL512444 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
POT2_ARATH	1.796e-26	53.93	Potassium transporter 2 OS=Arabidopsis thaliana GN...	[more]
HAK8_ORYSJ	2.184e-19	54.41	Putative potassium transporter 8 OS=Oryza sativa s...	[more]
HAK9_ORYSJ	1.893e-17	51.47	Probable potassium transporter 9 OS=Oryza sativa s...	[more]
POT4_ARATH	2.116e-15	42.86	Potassium transporter 4 OS=Arabidopsis thaliana GN...	[more]
POT8_ARATH	3.574e-15	45.78	Potassium transporter 8 OS=Arabidopsis thaliana GN...	[more]
HAK7_ORYSJ	4.639e-15	39.29	Potassium transporter 7 OS=Oryza sativa subsp. jap...	[more]
HAK10_ORYSJ	2.343e-13	50.72	Potassium transporter 10 OS=Oryza sativa subsp. ja...	[more]
POT1_ARATH	2.352e-13	53.33	Potassium transporter 1 OS=Arabidopsis thaliana GN...	[more]
HAK3_ORYSJ	5.135e-13	42.86	Probable potassium transporter 3 OS=Oryza sativa s...	[more]
HAK2_ORYSJ	9.073e-12	32.14	Probable potassium transporter 2 OS=Oryza sativa s...	[more]

BLAST of FL512444 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A5ACL8_VITVI	3.612e-36	74.70	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q06XL8_VITVI	2.299e-35	74.70	KUP2 OS=Vitis vinifera PE=2 SV=1	[more]
D7T4E8_VITVI	2.995e-35	72.29	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9N126_POPTR	2.216e-32	67.47	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9S4D1_RICCO	3.758e-32	66.27	Potassium transporter, putative OS=Ricinus communi...	[more]
B9IQ44_POPTR	1.516e-30	63.86	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9P545_POPTR	1.542e-30	63.86	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7LF53_ARALY	3.677e-25	53.93	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q94KC6_MESCR	5.127e-24	55.42	Potassium transporter HAK2p OS=Mesembryanthemum cr...	[more]
A2XGG4_ORYSI	4.471e-18	54.41	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FL512444 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT2G40540.2	1.533e-27	53.93	\| Symbols: KT2, ATKT2, SHY3, KUP2, ATKUP2, TRK2 \| ...	[more]
AT2G40540.1	1.533e-27	53.93	\| Symbols: KT2, ATKT2, SHY3, KUP2, ATKUP2, TRK2 \| ...	[more]
AT3G02050.1	1.807e-16	42.86	\| Symbols: KUP3, ATKUP3, ATKT4 \| K+ uptake transpo...	[more]
AT5G14880.1	3.050e-16	45.78	\| Symbols: \| Potassium transporter family protein...	[more]
AT2G30070.1	2.008e-14	53.33	\| Symbols: ATKT1, ATKT1P, ATKUP1, KUP1, KT1 \| pota...	[more]
AT1G70300.1	1.415e-10	39.13	\| Symbols: KUP6 \| K+ uptake permease 6 \| chr1:2647...	[more]
AT4G23640.1	8.992e-9	44.68	\| Symbols: TRH1, ATKT3, KUP4 \| Potassium transport...	[more]
AT5G09400.1	4.234e-8	36.51	\| Symbols: KUP7 \| K+ uptake permease 7 \| chr5:2916...	[more]
AT4G33530.1	7.247e-7	34.33	\| Symbols: KUP5 \| K+ uptake permease 5 \| chr4:1612...	[more]
AT1G60160.1	7.257e-7	40.00	\| Symbols: \| Potassium transporter family protein...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003855	K+ potassium transporter	PFAM	PF02705	K_trans	coord: 14..83 score: 2.6

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FL512444 ID=FL512444; Name=FL512444; organism=Cicer arietinum; type=EST; length=364bp
GAGCATGACAGATGTTATTGAAATGGAACAGATGGATGTAACCCAACGAC
GAGTGAGGTTTGCTATTGATGATGATCATGAAAGTGATACAAGGTCTGAG
GTTGGTGTTCAAATGCAGGAAGAGCTGGAAGATCTTTATGCTGCTCAAGA
AGCTGGCATTGCATTCATTCTTGGACATTCTTATGTGAGAGCAAAGCAAG
GCTCATCACTGTTGAGGAAATTGGCACTTAACTATGGATACAATTTCTTG
GGAGTTGTAGAGGAACTGACGTAGCGCTTAAGGTTCCACCCGTTTCTCTA
CTGGAAGTTGGCATGGTTTATATTGCGTAGAAAAAGACTAACATGTATTT
TTGTTGTGTTTTTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006813	potassium ion transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0015079	potassium ion transmembrane transporter activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR003855	K+_transporter

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