GR398412

EST Overview


Name	GR398412
Unique Name	GR398412
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	645

Libraries


Library Name	Type
LIBEST_024782 ICC4958 field drought stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR398412 vs. SwissProt
Match: P4HA1_PONAB (Prolyl 4-hydroxylase subunit alpha-1 OS=Pongo abelii GN=P4HA1 PE=2 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 7.286e-19
Identity = 57/205 (27.80%), Postives = 102/205 (49.76%), Query Frame = 2

Query:   41 IINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYF----TDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            I+ P+K +     PR   +   ++D E + +  LAK  L+R+ +++ ++GD +    R S   ++S  ++P+V+ I  +I   T L     E++QV  Y  G +Y+PH+D+      D       G+R  T L Y+++V+ GG T+FP                     + G +V P++G A+ +++L  +   D S+ HA CPV
Sbjct:  322 ILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFP---------------------EVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPV 505

[Close]

BLAST of GR398412 vs. SwissProt
Match: P4HA1_HUMAN (Prolyl 4-hydroxylase subunit alpha-1 OS=Homo sapiens GN=P4HA1 PE=1 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 7.286e-19
Identity = 57/205 (27.80%), Postives = 102/205 (49.76%), Query Frame = 2

Query:   41 IINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYF----TDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            I+ P+K +     PR   +   ++D E + +  LAK  L+R+ +++ ++GD +    R S   ++S  ++P+V+ I  +I   T L     E++QV  Y  G +Y+PH+D+      D       G+R  T L Y+++V+ GG T+FP                     + G +V P++G A+ +++L  +   D S+ HA CPV
Sbjct:  322 ILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFP---------------------EVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPV 505

[Close]

BLAST of GR398412 vs. SwissProt
Match: P4HA1_BOVIN (Prolyl 4-hydroxylase subunit alpha-1 OS=Bos taurus GN=P4HA1 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.243e-18
Identity = 57/205 (27.80%), Postives = 101/205 (49.27%), Query Frame = 2

Query:   41 IINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYF----TDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            I+ P+K +     PR   +   ++D E + +  LAK  L+R+ +++ ++GD +    R S   ++S  ++P+V+ I  +I   T L     E++QV  Y  G +Y+PH+D+      D       G+R  T L Y+++V  GG T+FP                     + G +V P++G A+ +++L  +   D S+ HA CPV
Sbjct:  322 ILAPAKQEDEWDKPRIIRFHDIISDAEIEVVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVLAGGATVFP---------------------EVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPV 505

[Close]

BLAST of GR398412 vs. SwissProt
Match: P4HA1_RAT (Prolyl 4-hydroxylase subunit alpha-1 OS=Rattus norvegicus GN=P4ha1 PE=2 SV=2)

HSP 1 Score: 92.8189 bits (229), Expect = 1.623e-18
Identity = 59/205 (28.78%), Postives = 98/205 (47.80%), Query Frame = 2

Query:   41 IINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYF----TDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            I+ P+K +     PR   +   ++D E + +  LAK  L R+ V D  +G    +  R S   ++S  +DP+V+ I  +I   T L     E++QV  Y  G +Y+PH+D+      D       G+R  T L Y+++V+ GG T+FP                     + G +V P++G A+ +++L  +   D S+ HA CPV
Sbjct:  322 ILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLSRATVHDPETGKLTTAQYRVSKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFP---------------------EVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPV 505

[Close]

BLAST of GR398412 vs. SwissProt
Match: P4HA1_MOUSE (Prolyl 4-hydroxylase subunit alpha-1 OS=Mus musculus GN=P4ha1 PE=2 SV=2)

HSP 1 Score: 92.4337 bits (228), Expect = 2.120e-18
Identity = 57/205 (27.80%), Postives = 101/205 (49.27%), Query Frame = 2

Query:   41 IINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYF----TDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            I+ P+K +     PR   +   ++D E + +  LAK  L+R+ +++ ++G  +    R S   ++S  +DP+V+ I  +I   T L     E++QV  Y  G +Y+PH+D+      D       G+R  T L Y+++V+ GG T+FP                     + G +V P++G A+ +++L  +   D S+ HA CPV
Sbjct:  322 ILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPVTGALETVHYRISKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFP---------------------EVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPV 505

[Close]

BLAST of GR398412 vs. SwissProt
Match: P4HA2_CAEEL (Prolyl 4-hydroxylase subunit alpha-2 OS=Caenorhabditis elegans GN=phy-2 PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 2.769e-18
Identity = 56/202 (27.72%), Postives = 98/202 (48.51%), Query Frame = 2

Query:   50 PSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIA----QGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            P KV+ + + P A +++  + D E + +  LA  +LKR+ V ++ +G+ + +  R S   ++  + DP++  +  +I  +T L +   E++QV  Y  G  YDPH+D+   +   A      G+R  TVL Y++   +GG T+F                        G AV P + DAL +++L  +   D  + HA CPV
Sbjct:  315 PIKVEILRFDPLAVLFKNVIHDSEIEVIKELASPKLKRATVQNSKTGELEHATYRISKSAWLKGDLDPVIDRVNRRIEDFTNLNQATSEELQVANYGLGGHYDPHFDFARKEEKNAFKTLNTGNRIATVLFYMSQPERGGATVF---------------------NHLGTAVFPSKNDALFWYNLRRDGEGDLRTRHAACPV 495

[Close]

BLAST of GR398412 vs. SwissProt
Match: P4HA1_CHICK (Prolyl 4-hydroxylase subunit alpha-1 OS=Gallus gallus GN=P4HA1 PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 3.998e-17
Identity = 58/205 (28.29%), Postives = 96/205 (46.83%), Query Frame = 2

Query:   41 IINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDY----FTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            I+ P K +     PR   +   ++D E + +  LAK  L R+ V D  +G    +  R S   ++S  + P+V+ I  +I   T L     E++QV  Y  G +Y+PH+D+      D       G+R  T L Y+++V+ GG T+FP                     + G +V P++G A+ +++L  +   D S+ HA CPV
Sbjct:  304 ILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLSRATVHDPETGKLTTAHYRVSKSAWLSGYESPVVSRINTRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFP---------------------EVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPV 487

[Close]

BLAST of GR398412 vs. SwissProt
Match: P4HA2_CHICK (Prolyl 4-hydroxylase subunit alpha-2 OS=Gallus gallus GN=P4HA2 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 5.221e-17
Identity = 55/203 (27.09%), Postives = 96/203 (47.29%), Query Frame = 2

Query:   41 IINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVN--IAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            +I P K +     P    Y   ++D E + +  LAK +L R+ V D  +G   ++  R S   ++ ++ DP+VA +  ++   T L  +  E +QV  Y  G +Y+PH+D+     +  +   G+R  T L Y+++V  GG T+FP                       G A+ P++G A+ +++L  +   D  + HA CPV
Sbjct:  324 LIAPFKEEDEWDSPHIVRYYDVMSDEEIEKIKQLAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVAKVNQRMQQITGLTVKTAELLQVANYGMGGQYEPHFDFSRRPFDSTLKSEGNRLATFLNYMSDVEAGGATVFP---------------------DFGAAIWPKKGTAVFWYNLFRSGEGDYRTRHAACPV 505

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BLAST of GR398412 vs. SwissProt
Match: P4H_MIMIV (Putative prolyl 4-hydroxylase OS=Acanthamoeba polyphaga mimivirus GN=MIMI_L593 PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 8.906e-17
Identity = 65/193 (33.68%), Postives = 91/193 (47.15%), Query Frame = 2

Query:   83 RAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVN-----IAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATP-DTSSLHAGCPV 643
            + FV    +   +C  ++  A  +L  S V   LSG  K  ++R S  M+ISKN +P+V  I + I     +P +N ED+QV+RY   Q Y+ H+D   D        I +GG R +TVL+YL N    G T FP   +                       KP+ GDAL+F+ L  N+      SLHAG PV
Sbjct:   59 KPFVLNNLINPTKCQEIMQFANGKLFDSQV---LSGTDK--NIRNSQQMWISKN-NPMVKPIFENICRQFNVPFDNAEDLQVVRYLPNQYYNEHHDSCCDSSKQCSEFIERGGQRILTVLIYLNNEFSDGHTYFPNLNQ---------------------KFKPKTGDALVFYPLANNSNKCHPYSLHAGMPV 224

[Close]

BLAST of GR398412 vs. SwissProt
Match: P4HA2_MOUSE (Prolyl 4-hydroxylase subunit alpha-2 OS=Mus musculus GN=P4ha2 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.591e-16
Identity = 57/205 (27.80%), Postives = 97/205 (47.32%), Query Frame = 2

Query:   41 IINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYF----TDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            +I P K +     P    Y   ++D E + +  +AK +L R+ V D  +G   ++  R S   ++ ++ DP+VA +  ++   T L  +  E +QV  Y  G +Y+PH+D+      D       G+R  T L Y+++V  GG T+FP               DL      G A+ P++G A+ +++L  +   D  + HA CPV
Sbjct:  325 LIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKTAELLQVANYGMGGQYEPHFDFSRSDDEDAFKRLGTGNRVATFLNYMSDVEAGGATVFP---------------DL------GAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPV 508

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BLAST of GR398412 vs. TrEMBL
Match: C6TG86_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 403.29 bits (1035), Expect = 8.116e-111
Identity = 197/214 (92.06%), Postives = 206/214 (96.26%), Query Frame = 2

Query:    2 DEVHSSYAGSASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            D V SSYAGSASS+INPSKVKQISW PRAFVY+GFLTDLECDHLISLAKSELKRSAVADNLSG+S+LSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQV RYEHGQKYDPHYDYFTDKVNIA+GGHR  TVLMYLT+V KGGET+FP A+EPPRRRG+ETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV
Sbjct:   17 DHVWSSYAGSASSVINPSKVKQISWKPRAFVYEGFLTDLECDHLISLAKSELKRSAVADNLSGESQLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVSRYEHGQKYDPHYDYFTDKVNIARGGHRIATVLMYLTDVAKGGETVFPSAEEPPRRRGAETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 230

[Close]

BLAST of GR398412 vs. TrEMBL
Match: C6TJY4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 402.905 bits (1034), Expect = 1.060e-110
Identity = 195/213 (91.55%), Postives = 206/213 (96.71%), Query Frame = 2

Query:    5 EVHSSYAGSASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            +V  SYAGSASSI+NPSKVKQISW PRAFVY+GFLTDLECDHLISLAKSELKRSAVADNLSG+S+LSDVRTSSGMFISKNKDPI++GIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIA+GGHR  TVLMYLTNVTKGGET+FP A+EPPRRRG+ETSSDLSECAKKGIAVKP RGDALLFFSLHTNATPDTSSLHAGCPV
Sbjct:   19 QVWGSYAGSASSIVNPSKVKQISWKPRAFVYEGFLTDLECDHLISLAKSELKRSAVADNLSGESQLSDVRTSSGMFISKNKDPIISGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIARGGHRIATVLMYLTNVTKGGETVFPSAEEPPRRRGTETSSDLSECAKKGIAVKPHRGDALLFFSLHTNATPDTSSLHAGCPV 231

[Close]

BLAST of GR398412 vs. TrEMBL
Match: C6THC6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 371.703 bits (953), Expect = 2.617e-101
Identity = 181/213 (84.98%), Postives = 198/213 (92.96%), Query Frame = 2

Query:    5 EVHSSYAGSASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            E  SSYAGSAS+II+PSKVKQ+SW PRAFVY+GFLT+LECDHLIS+AKSELKRSAVADNLSG+SKLS+VRTSSGMFI KNKD IVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYF DKVNIA+GGHR  TVLMYLT+VTKGGET+FP A+E PR +GSET+ +LSECA+KGIAVKPRRGDALLFFSL+ NA PDT SLHAGCPV
Sbjct:   22 EAFSSYAGSASAIIDPSKVKQVSWKPRAFVYEGFLTELECDHLISIAKSELKRSAVADNLSGESKLSEVRTSSGMFIPKNKDLIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFADKVNIARGGHRVATVLMYLTDVTKGGETVFPDAEESPRHKGSETNENLSECAQKGIAVKPRRGDALLFFSLYPNAIPDTLSLHAGCPV 234

[Close]

BLAST of GR398412 vs. TrEMBL
Match: B9RSW4_RICCO (Prolyl 4-hydroxylase alpha subunit, putative OS=Ricinus communis GN=RCOM_0679070 PE=4 SV=1)

HSP 1 Score: 360.147 bits (923), Expect = 7.879e-98
Identity = 171/210 (81.43%), Postives = 192/210 (91.43%), Query Frame = 2

Query:   14 SSYAGSASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            SSY GS +SII+PSKVKQ+SW PRAFVY+GFLTDLECDHLISLAKSELKRSAVADN SG SKLS+VRTSSGMFI+K KDPI+AGIE+KIS+WTFLPKENGED+QVLRYEHGQKYDPHYDYF DK+NIA+GGHR  TVLMYL++V KGGET+FP A+EPPRR+ +E+  DLSECAKKGI+VKPRRGDALLFFSLH  A PD +SLHAGCPV
Sbjct:   21 SSYPGSPTSIIDPSKVKQVSWKPRAFVYEGFLTDLECDHLISLAKSELKRSAVADNESGKSKLSEVRTSSGMFIAKGKDPIIAGIEEKISTWTFLPKENGEDLQVLRYEHGQKYDPHYDYFADKINIARGGHRMATVLMYLSDVVKGGETVFPNAEEPPRRKATESHEDLSECAKKGISVKPRRGDALLFFSLHPTAIPDPNSLHAGCPV 230

[Close]

BLAST of GR398412 vs. TrEMBL
Match: B9HM91_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1085714 PE=4 SV=1)

HSP 1 Score: 357.066 bits (915), Expect = 6.670e-97
Identity = 172/209 (82.30%), Postives = 192/209 (91.87%), Query Frame = 2

Query:   17 SYAGSASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            SY G++SSIINP+KVKQ+SW PRAFVY+GFLTDLECDHLISLAKSELKRSAVADN SG SKLS+VRTSSGMFI+K KDPIVAGIEDKI++WTFLP+ENGEDIQVLRYEHGQKYDPHYDYF+DKVNIA+GGHR  TVLMYLT+V KGGET+FP A+E PRR+ S +  DLSECA+KGIAVKPRRGDALLFFSL+  A PDTSS+HAGCPV
Sbjct:   25 SYPGTSSSIINPAKVKQVSWKPRAFVYEGFLTDLECDHLISLAKSELKRSAVADNESGKSKLSEVRTSSGMFITKAKDPIVAGIEDKIATWTFLPRENGEDIQVLRYEHGQKYDPHYDYFSDKVNIARGGHRVATVLMYLTDVEKGGETVFPSAEELPRRKASVSHEDLSECARKGIAVKPRRGDALLFFSLYPTAVPDTSSIHAGCPV 233

[Close]

BLAST of GR398412 vs. TrEMBL
Match: B6ZN57_TOBAC (Type 2 proly 4-hydroxylase OS=Nicotiana tabacum GN=NtP4H2.1 PE=2 SV=1)

HSP 1 Score: 333.954 bits (855), Expect = 6.047e-90
Identity = 162/205 (79.02%), Postives = 179/205 (87.32%), Query Frame = 2

Query:   29 SASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            S+S+IINPSK KQISW PRAFVY+GFLTD EC+HLISLAKSELKRSAVADN SG+SK S+VRTSSGMFI K KDPIV+GIE+KI++WTFLPKENGE+IQVLRYE GQKY+PHYDYF DKVNIA+GGHR  TVLMYLTNV KGGET+FP A+E PRRR       LSECAKKGI VKPR+GDALLF+SLH NATPD  SLH GCPV
Sbjct:   23 SSSAIINPSKAKQISWKPRAFVYEGFLTDEECNHLISLAKSELKRSAVADNESGNSKTSEVRTSSGMFIPKAKDPIVSGIEEKIATWTFLPKENGEEIQVLRYEEGQKYEPHYDYFVDKVNIARGGHRLATVLMYLTNVEKGGETVFPKAEESPRRRSMIADDSLSECAKKGIPVKPRKGDALLFYSLHPNATPDPLSLHGGCPV 227

[Close]

BLAST of GR398412 vs. TrEMBL
Match: D7T6M1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_20.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00017841001 PE=4 SV=1)

HSP 1 Score: 332.798 bits (852), Expect = 1.347e-89
Identity = 160/213 (75.12%), Postives = 185/213 (86.85%), Query Frame = 2

Query:    5 EVHSSYAGSASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            E  SSYA +A S ++ +KV+QISW PRAFVY+GFL++ ECDHLISLAKSELKRSAVADN+SG S+LS+VRTSSGMFI K KDPIVAGIEDKI++WTFLPK+NGED+QVLRYE GQKYD HYDYF DKVNIA+GGHR  TVLMYL++V KGGET+FP+A+EP RR+   T+ DLSECA+KGIAVKPR+GDALLFFSLH  A PD  SLH GCPV
Sbjct:   19 EFSSSYADAAGSNVSAAKVRQISWKPRAFVYEGFLSEEECDHLISLAKSELKRSAVADNVSGKSRLSEVRTSSGMFIGKGKDPIVAGIEDKIAAWTFLPKDNGEDMQVLRYEPGQKYDAHYDYFVDKVNIARGGHRIATVLMYLSDVVKGGETVFPMAEEPSRRKPLPTNDDLSECARKGIAVKPRKGDALLFFSLHPTAIPDPMSLHGGCPV 231

[Close]

BLAST of GR398412 vs. TrEMBL
Match: Q8RWK0_ARATH (Putative uncharacterized protein At5g18900 OS=Arabidopsis thaliana GN=At5g18900 PE=2 SV=1)

HSP 1 Score: 324.324 bits (830), Expect = 4.791e-87
Identity = 156/210 (74.29%), Postives = 180/210 (85.71%), Query Frame = 2

Query:   14 SSYAGSASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            +S   S+S  +NPSKVKQ+S  PRAFVY+GFLT+LECDH++SLAK+ LKRSAVADN SG+SK S+VRTSSG FISK KDPIV+GIEDKIS+WTFLPKENGEDIQVLRYEHGQKYD H+DYF DKVNI +GGHR  T+LMYL+NVTKGGET+FP A+ P RR  SE   DLS+CAK+GIAVKPR+GDALLFF+LH +A PD  SLH GCPV
Sbjct:   22 TSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENEEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPV 231

[Close]

BLAST of GR398412 vs. TrEMBL
Match: Q8LAN3_ARATH (Prolyl 4-hydroxylase alpha subunit-like protein OS=Arabidopsis thaliana GN=At5g18900 PE=2 SV=1)

HSP 1 Score: 324.324 bits (830), Expect = 4.791e-87
Identity = 156/210 (74.29%), Postives = 180/210 (85.71%), Query Frame = 2

Query:   14 SSYAGSASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            +S   S+S  +NPSKVKQ+S  PRAFVY+GFLT+LECDH++SLAK+ LKRSAVADN SG+SK S+VRTSSG FISK KDPIV+GIEDKIS+WTFLPKENGEDIQVLRYEHGQKYD H+DYF DKVNI +GGHR  T+LMYL+NVTKGGET+FP A+ P RR  SE   DLS+CAK+GIAVKPR+GDALLFF+LH +A PD  SLH GCPV
Sbjct:   22 TSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPV 231

[Close]

BLAST of GR398412 vs. TrEMBL
Match: Q8L8T9_ARATH (Prolyl 4-hydroxylase alpha subunit-like protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 320.472 bits (820), Expect = 6.919e-86
Identity = 156/205 (76.10%), Postives = 179/205 (87.32%), Query Frame = 2

Query:   29 SASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            S SSIINPSKVKQ+S  PRAFVY+GFLTDLECDHLISLAK  L+RSAVADN +G+S++SDVRTSSG FISK KDPIV+GIEDK+S+WTFLPKENGED+QVLRYEHGQKYD H+DYF DKVNIA+GGHR  TVL+YL+NVTKGGET+FP A+E  RR  SE   DLS+CAKKGIAVKP++G+ALLFF+L  +A PD  SLH GCPV
Sbjct:   26 SPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPV 230

[Close]

BLAST of GR398412 vs. TAIR peptide
Match: AT5G18900.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr5:6304409-6306166 REVERSE LENGTH=298)

HSP 1 Score: 324.324 bits (830), Expect = 3.398e-89
Identity = 156/210 (74.29%), Postives = 180/210 (85.71%), Query Frame = 2

Query:   14 SSYAGSASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            +S   S+S  +NPSKVKQ+S  PRAFVY+GFLT+LECDH++SLAK+ LKRSAVADN SG+SK S+VRTSSG FISK KDPIV+GIEDKIS+WTFLPKENGEDIQVLRYEHGQKYD H+DYF DKVNI +GGHR  T+LMYL+NVTKGGET+FP A+ P RR  SE   DLS+CAK+GIAVKPR+GDALLFF+LH +A PD  SLH GCPV
Sbjct:   22 TSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPV 231

[Close]

BLAST of GR398412 vs. TAIR peptide
Match: AT3G06300.1 (| Symbols: AT-P4H-2 | P4H isoform 2 | chr3:1907744-1909675 FORWARD LENGTH=299)

HSP 1 Score: 320.472 bits (820), Expect = 4.906e-88
Identity = 156/205 (76.10%), Postives = 179/205 (87.32%), Query Frame = 2

Query:   29 SASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            S SSIINPSKVKQ+S  PRAFVY+GFLTDLECDHLISLAK  L+RSAVADN +G+S++SDVRTSSG FISK KDPIV+GIEDK+S+WTFLPKENGED+QVLRYEHGQKYD H+DYF DKVNIA+GGHR  TVL+YL+NVTKGGET+FP A+E  RR  SE   DLS+CAKKGIAVKP++G+ALLFF+L  +A PD  SLH GCPV
Sbjct:   28 SPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPV 232

[Close]

BLAST of GR398412 vs. TAIR peptide
Match: AT3G28480.1 (| Symbols: | Oxoglutarate/iron-dependent oxygenase | chr3:10676266-10678262 REVERSE LENGTH=316)

HSP 1 Score: 258.84 bits (660), Expect = 1.753e-69
Identity = 123/206 (59.71%), Postives = 162/206 (78.64%), Query Frame = 2

Query:   29 SASSI-INPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            SASS   +P++V Q+SW PR F+Y+GFL+D ECDH I LAK +L++S VADN SG+S  S+VRTSSGMF+SK +D IV+ +E K+++WTFLP+ENGE +Q+L YE+GQKY+PH+DYF D+ N+  GGHR  TVLMYL+NV KGGET+FP+ K    +   ++    +ECAK+G AVKPR+GDALLFF+LH NAT D++SLH  CPV
Sbjct:   45 SASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDS---WTECAKQGYAVKPRKGDALLFFNLHPNATTDSNSLHGSCPV 247

[Close]

BLAST of GR398412 vs. TAIR peptide
Match: AT3G28490.1 (| Symbols: | Oxoglutarate/iron-dependent oxygenase | chr3:10679150-10680654 REVERSE LENGTH=288)

HSP 1 Score: 252.292 bits (643), Expect = 1.641e-67
Identity = 124/211 (58.77%), Postives = 162/211 (76.78%), Query Frame = 2

Query:   17 SYAGSASSIINPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRS-AVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAK-EPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            S   S S  ++P+++ Q+SW PRAF+Y+GFL+D ECDHLI LAK +L++S  VAD  SG+S+ S+VRTSSGMF++K +D IVA +E K+++WTFLP+ENGE +Q+L YE+GQKYDPH+DYF DK  +  GGHR  TVLMYL+NVTKGGET+FP  K + P+ +        S+CAK+G AVKPR+GDALLFF+LH N T D +SLH  CPV
Sbjct:   18 SQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYDKKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLK----DDSWSKCAKQGYAVKPRKGDALLFFNLHLNGTTDPNSLHGSCPV 224

[Close]

BLAST of GR398412 vs. TAIR peptide
Match: AT3G28480.2 (| Symbols: | Oxoglutarate/iron-dependent oxygenase | chr3:10676266-10678262 REVERSE LENGTH=324)

HSP 1 Score: 234.187 bits (596), Expect = 4.624e-62
Identity = 117/214 (54.67%), Postives = 156/214 (72.90%), Query Frame = 2

Query:   29 SASSI-INPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDS-----KLSDVRTSSGMFISKNK---DPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            SASS   +P++V Q+SW PR F+Y+GFL+D ECDH I LAK +L++S VADN SG+S      +S VR SS    + +    D IV+ +E K+++WTFLP+ENGE +Q+L YE+GQKY+PH+DYF D+ N+  GGHR  TVLMYL+NV KGGET+FP+ K    +   ++    +ECAK+G AVKPR+GDALLFF+LH NAT D++SLH  CPV
Sbjct:   45 SASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSVVRQSSSFIANMDSLEIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDS---WTECAKQGYAVKPRKGDALLFFNLHPNATTDSNSLHGSCPV 255

[Close]

BLAST of GR398412 vs. TAIR peptide
Match: AT1G20270.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr1:7021383-7022923 REVERSE LENGTH=287)

HSP 1 Score: 211.846 bits (538), Expect = 2.458e-55
Identity = 103/192 (53.65%), Postives = 137/192 (71.35%), Query Frame = 2

Query:   68 ISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            +SW PRAFVY  FL+  EC++LISLAK  + +S V D+ +G SK S VRTSSG F+ + +D I+  IE +I+ +TF+P ++GE +QVL YE GQKY+PHYDYF D+ N   GG R  T+LMYL++V +GGET+FP A            ++LSEC KKG++VKPR GDALLF+S+  +AT D +SLH GCPV
Sbjct:   79 LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAAN--MNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDATLDPTSLHGGCPV 268

[Close]

BLAST of GR398412 vs. TAIR peptide
Match: AT4G35810.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr4:16968921-16970358 FORWARD LENGTH=290)

HSP 1 Score: 208.764 bits (530), Expect = 2.081e-54
Identity = 105/196 (53.57%), Postives = 141/196 (71.94%), Query Frame = 2

Query:   68 ISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETS----SDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            ISW PRAFVY  FLT+ EC+HLISLAK  + +S V D  +G S  S VRTSSG F+++  D IV  IE++IS +TF+P ENGE +QVL YE GQ+Y+PH+DYF D+ N+ +GG R  TVLMYL++V +GGET+FP AK      G+ +      +LS+C K+G++V P++ DALLF+S+  +A+ D SSLH GCPV
Sbjct:   83 ISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKTGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPHHDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAK------GNVSDVPWWDELSQCGKEGLSVLPKKRDALLFWSMKPDASLDPSSLHGGCPV 272

[Close]

BLAST of GR398412 vs. TAIR peptide
Match: AT2G17720.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr2:7697513-7699174 FORWARD LENGTH=291)

HSP 1 Score: 207.608 bits (527), Expect = 4.635e-54
Identity = 105/199 (52.76%), Postives = 140/199 (70.35%), Query Frame = 2

Query:   59 VKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETS----SDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            V+ ISW PRA VY  FLT+ EC+HLISLAK  + +S V D  +G SK S VRTSSG F+ +  D +V  IE +IS +TF+P ENGE +QVL Y+ GQKY+PHYDYF D+ N   GG R  TVLMYL++V  GGET+FP A      RG+ ++    ++LS+C K+G++V P++ DALLF+++  +A+ D SSLH GCPV
Sbjct:   80 VEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFLDEFNTKNGGQRIATVLMYLSDVDDGGETVFPAA------RGNISAVPWWNELSKCGKEGLSVLPKKRDALLFWNMRPDASLDPSSLHGGCPV 272

[Close]

BLAST of GR398412 vs. TAIR peptide
Match: AT5G66060.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr5:26419481-26421118 FORWARD LENGTH=289)

HSP 1 Score: 204.912 bits (520), Expect = 3.004e-53
Identity = 106/203 (52.22%), Postives = 139/203 (68.47%), Query Frame = 2

Query:   47 NPSKVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETS----SDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            N   V+ ISW PRA VY  FLT  EC +LI LAK  +++S V D  +G S  S VRTSSG F+++ +D  +  IE +IS +TF+P E+GE +QVL YE GQKY+PHYDYF D+ N   GG R  TVLMYL++V +GGET+FP AK      G+ ++    ++LSEC K G++VKP+ GDALLF+S+  +AT D SSLH GC V
Sbjct:   74 NERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAK------GNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSMTPDATLDPSSLHGGCAV 270

[Close]

BLAST of GR398412 vs. TAIR peptide
Match: AT2G43080.1 (| Symbols: AT-P4H-1 | P4H isoform 1 | chr2:17915755-17918599 FORWARD LENGTH=283)

HSP 1 Score: 162.925 bits (411), Expect = 1.309e-40
Identity = 88/198 (44.44%), Postives = 123/198 (62.12%), Query Frame = 2

Query:   56 KVKQISWIPRAFVYQGFLTDLECDHLISLAKSELKRSAVADNLSGDSKLSDVRTSSGMFIS--KNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHGQKYDPHYDYFTDKVNIAQGGHRFVTVLMYLTNVTKGGETMFPVAKEPPRRRGSETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPV 643
            K + +SW PR  V   FL+  EC++L ++A+  L+ S V D  +G    SDVRTSSGMF++  +   PI+  IE +I+ ++ +P ENGE IQVLRYE  Q Y PH+DYF D  N+ +GG R  T+LMYLT+  +GGET FP+A +     G +          KGI+VKP +GDA+LF+S+  +   D  S+H GC V
Sbjct:   76 KPEVVSWSPRIIVLHDFLSPEECEYLKAIARPRLQVSTVVDVKTGKGVKSDVRTSSGMFLTHVERSYPIIQAIEKRIAVFSQVPAENGELIQVLRYEPQQFYKPHHDYFADTFNLKRGGQRVATMLMYLTDDVEGGETYFPLAGDGDCTCGGK--------IMKGISVKPTKGDAVLFWSMGLDGQSDPRSIHGGCEV 265

The following BLAST results are available for this feature:

BLAST of GR398412 vs. SwissProt
BLAST of GR398412 vs. TrEMBL
BLAST of GR398412 vs. TAIR peptide

BLAST of GR398412 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
P4HA1_PONAB	7.286e-19	27.80	Prolyl 4-hydroxylase subunit alpha-1 OS=Pongo abel...	[more]
P4HA1_HUMAN	7.286e-19	27.80	Prolyl 4-hydroxylase subunit alpha-1 OS=Homo sapie...	[more]
P4HA1_BOVIN	1.243e-18	27.80	Prolyl 4-hydroxylase subunit alpha-1 OS=Bos taurus...	[more]
P4HA1_RAT	1.623e-18	28.78	Prolyl 4-hydroxylase subunit alpha-1 OS=Rattus nor...	[more]
P4HA1_MOUSE	2.120e-18	27.80	Prolyl 4-hydroxylase subunit alpha-1 OS=Mus muscul...	[more]
P4HA2_CAEEL	2.769e-18	27.72	Prolyl 4-hydroxylase subunit alpha-2 OS=Caenorhabd...	[more]
P4HA1_CHICK	3.998e-17	28.29	Prolyl 4-hydroxylase subunit alpha-1 OS=Gallus gal...	[more]
P4HA2_CHICK	5.221e-17	27.09	Prolyl 4-hydroxylase subunit alpha-2 OS=Gallus gal...	[more]
P4H_MIMIV	8.906e-17	33.68	Putative prolyl 4-hydroxylase OS=Acanthamoeba poly...	[more]
P4HA2_MOUSE	2.591e-16	27.80	Prolyl 4-hydroxylase subunit alpha-2 OS=Mus muscul...	[more]

BLAST of GR398412 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TG86_SOYBN	8.116e-111	92.06	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TJY4_SOYBN	1.060e-110	91.55	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6THC6_SOYBN	2.617e-101	84.98	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9RSW4_RICCO	7.879e-98	81.43	Prolyl 4-hydroxylase alpha subunit, putative OS=Ri...	[more]
B9HM91_POPTR	6.670e-97	82.30	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B6ZN57_TOBAC	6.047e-90	79.02	Type 2 proly 4-hydroxylase OS=Nicotiana tabacum GN...	[more]
D7T6M1_VITVI	1.347e-89	75.12	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q8RWK0_ARATH	4.791e-87	74.29	Putative uncharacterized protein At5g18900 OS=Arab...	[more]
Q8LAN3_ARATH	4.791e-87	74.29	Prolyl 4-hydroxylase alpha subunit-like protein OS...	[more]
Q8L8T9_ARATH	6.919e-86	76.10	Prolyl 4-hydroxylase alpha subunit-like protein OS...	[more]

BLAST of GR398412 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G18900.1	3.398e-89	74.29	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT3G06300.1	4.906e-88	76.10	\| Symbols: AT-P4H-2 \| P4H isoform 2 \| chr3:1907744...	[more]
AT3G28480.1	1.753e-69	59.71	\| Symbols: \| Oxoglutarate/iron-dependent oxygenas...	[more]
AT3G28490.1	1.641e-67	58.77	\| Symbols: \| Oxoglutarate/iron-dependent oxygenas...	[more]
AT3G28480.2	4.624e-62	54.67	\| Symbols: \| Oxoglutarate/iron-dependent oxygenas...	[more]
AT1G20270.1	2.458e-55	53.65	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT4G35810.1	2.081e-54	53.57	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT2G17720.1	4.635e-54	52.76	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT5G66060.1	3.004e-53	52.22	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT2G43080.1	1.309e-40	44.44	\| Symbols: AT-P4H-1 \| P4H isoform 1 \| chr2:1791575...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent oxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 104..214 score: 2.9
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	no description	coord: 27..214 score: 1.1
None	No IPR available	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 10..214 score: 2.6
None	No IPR available	PROFILE	PS51471	FE2OG_OXY	coord: 103..214 score: 10

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR398412 ID=GR398412; Name=GR398412; organism=Cicer arietinum; type=EST; length=645bp
GGATGAAGTTCATAGCTCCTACGCTGGTTCAGCCAGTTCCATCATCAATC
CTTCTAAAGTCAAACAGATTTCATGGATTCCAAGAGCTTTTGTTTACCAA
GGTTTTCTCACCGATTTGGAATGCGATCACTTGATCTCCTTAGCGAAATC
GGAGCTGAAGAGATCTGCCGTTGCTGATAATCTATCCGGTGATAGCAAGT
TAAGTGATGTTCGAACAAGCTCTGGCATGTTCATTTCCAAAAACAAGGAT
CCTATTGTTGCTGGTATTGAGGACAAGATTTCATCTTGGACCTTTCTTCC
TAAAGAAAATGGGGAAGACATACAAGTATTGAGATATGAGCATGGACAGA
AATATGACCCACATTATGATTATTTTACTGATAAAGTTAACATAGCTCAA
GGTGGGCATCGCTTCGTGACTGTTCTCATGTATCTCACTAATGTGACTAA
AGGTGGTGAGACTATGTTCCCAGTTGCAAAGGAACCTCCACGTCGCAGAG
GTTCTGAAACAAGTAGTGATCTCTCTGAGTGTGCGAAAAAAGGAATAGCA
GTGAAACCGCGTAGAGGGGATGCGCTTCTTTTCTTCAGTCTTCACACAAA
TGCTACACCAGACACTAGCAGTCTCCATGCAGGATGCCCAGTAAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0005506	iron ion binding
GO:0031418	L-ascorbic acid binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR006620	Pro_4_hyd_alph

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