GR398650

EST Overview


Name	GR398650
Unique Name	GR398650
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	800

Libraries


Library Name	Type
LIBEST_024782 ICC4958 field drought stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR398650 vs. SwissProt
Match: DIOXL_ARATH (4,5-DOPA dioxygenase extradiol-like protein OS=Arabidopsis thaliana GN=At4g15093 PE=2 SV=1)

HSP 1 Score: 264.618 bits (675), Expect = 1.133e-72
Identity = 125/201 (62.19%), Postives = 157/201 (78.11%), Query Frame = 3

Query:  144 LKETFYISHGSPTLAIDETIPAWKFLKQW-KEVFPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELI-EASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHR--DSPPPAWALAFDSWLKNHSLK 734
            + +TF++SHGSPTL+ID+++ A +F K W ++V P +P +ILVIS HWDT  P+VN V  N TI+DFSGFP  MYKLKY APGA +L ++VKEL+ +  G+ RVDED KRGLDHG WVPLMLMYPEADIP+CQLSV S ++G+YHYN+GKALA LKDEGVLI+GSGSATHN+R +     D  P  WAL FD WL++  L+
Sbjct:    4 VNQTFFLSHGSPTLSIDDSLEARQFFKSWTQKVLPQKPKSILVISAHWDTKFPSVNTVLRNNTIHDFSGFPDPMYKLKYEAPGAIELGKRVKELLMKEGGMKRVDEDTKRGLDHGAWVPLMLMYPEADIPICQLSVQSNQNGSYHYNMGKALASLKDEGVLIIGSGSATHNLRKLDFNITDGSPVPWALEFDHWLRDSLLQ 204

HSP 2 Score: 30.8018 bits (68), Expect = 1.133e-72
Identity = 12/21 (57.14%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHPW 795
            RY D+N ++EK P  K+AHPW
Sbjct:  206 RYGDVNEWEEKAPNAKMAHPW 226

[Close]

BLAST of GR398650 vs. SwissProt
Match: DODA_PORGR (4,5-DOPA dioxygenase extradiol OS=Portulaca grandiflora GN=DODA PE=2 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 6.356e-55
Identity = 100/202 (49.50%), Postives = 138/202 (68.32%), Query Frame = 3

Query:  132 EKMALKETFYISHGSPTLAIDETIPAWKFLKQWKE-VFPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHRDSPPPAWALAFDSWLKNHSLK 734
            ++++ KE+F++SHG+P +  DE+  A  FL  WK+ VFP +P +ILV+S HW+T VP V+  Q+   IYDF+  P SM+++KYPAPG P+LA++V+EL+ A G      D++RG DH +WVPL +M PEADIPVCQLSV    D T+H+N+G+ALAPLK EGVL +GSG A H      H       WA  FD WL++  L+
Sbjct:    5 KEVSFKESFFLSHGNPAMLADESFIARNFLLGWKKNVFPVKPKSILVVSAHWETDVPCVSAGQYPNVIYDFTEVPASMFQMKYPAPGCPKLAKRVQELLIAGGFKSAKLDEERGFDHSSWVPLSMMCPEADIPVCQLSVQPGLDATHHFNVGRALAPLKGEGVLFIGSGGAVHPSDDTPHWFDGVAPWAAEFDQWLEDALLE 206

HSP 2 Score: 22.7126 bits (47), Expect = 6.356e-55
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 1

Query:  736 RYDDINHYDEKPI--CKVAHP 792
            RY+D+N+Y  K     K+AHP
Sbjct:  208 RYEDVNNYQTKAPEGWKLAHP 228

[Close]

BLAST of GR398650 vs. SwissProt
Match: YGID_ECOLI (Uncharacterized protein ygiD OS=Escherichia coli (strain K12) GN=ygiD PE=1 SV=3)

HSP 1 Score: 152.91 bits (385), Expect = 1.932e-36
Identity = 75/192 (39.06%), Postives = 123/192 (64.06%), Query Frame = 3

Query:  159 YISHGSPTLAIDETIPAWKFLKQWKEV---FPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKR-GLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAI-GHRDSPPPAWALAFDSWLK 719
            ++ HGSP   +++ +    + + W+++    P RP AI+V+S HW T    V  ++   TI+DF GFP+++Y   YPAPG+P LA+++ EL+    ++    DK+  G DHG+W  L+ MYP+ADIP+ QLS+ S +   +H+ +G+ LA L+DEG+++V SG+  HN+R +  H DS P  WA +F+ ++K
Sbjct:   10 FLGHGSPMNVLEDNL----YTRSWQKLGMTLP-RPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVTL---DKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVK 193

[Close]

BLAST of GR398650 vs. SwissProt
Match: DIOXL_SCHPO (4,5-DOPA dioxygenase extradiol-like protein OS=Schizosaccharomyces pombe GN=SPBC1709.16c PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.760e-13
Identity = 54/195 (27.69%), Postives = 97/195 (49.74%), Query Frame = 3

Query:  117 VNSIIEKMALKETFYISHGSPTLAIDETIPA-------------WKFLKQWKEVF--PHRPSAILVISGHWDT--SVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEA-DIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATH 647
            + S+ +   L    Y++HGSP L + ++                ++FL+Q         RP  I+V S H+++  SV   +    N   YD+ GFP  +Y++K+ + G+ ++A ++   ++   +        RGLDHG +VP  +M+P+  +IP+ ++S+ +  D    Y +G+AL  L+ E  LIV  G   H
Sbjct:    1 MRSVPQLERLIPALYLAHGSPFLMLPQSSDDEVFSNDSKLGGLHYQFLEQLGPFLLEKFRPKGIIVFSAHYESRGSVEVYSRDDENPLFYDYYGFPDYLYQIKFHSKGSKRIADQIISALKEYQIPAKTVSGDRGLDHGVFVPFKIMFPDGLNIPLIEVSMHT-LDPMQLYKVGQALQSLRKE-YLIVSGGLNIH 193

[Close]

BLAST of GR398650 vs. TrEMBL
Match: C6TM42_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 317.775 bits (813), Expect = 1.229e-87
Identity = 147/198 (74.24%), Postives = 164/198 (82.83%), Query Frame = 3

Query:  138 MALKETFYISHGSPTLAIDETIPAWKFLKQWKEVFPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHRDSPPPAWALAFDSWLKNHSL 731
            + LKETFY+SHG PTLA+D++IPAW F   WKE FP RPS+IL+IS HWDT VPTVNVV  N TIYDF GFP+SMYKLKYPAPGAP LA++VKE +  SG S VDEDKKRGLDHG WVPL+LMYPEADIPVCQLS+SS + GTYHYN+GKAL PLKDEGVLIVGSGSATHN+RAI  R +PP  WA AF SWLK   L
Sbjct:   39 LRLKETFYLSHGVPTLAVDDSIPAWNFFNSWKEQFPTRPSSILIISAHWDTHVPTVNVVHQNDTIYDFYGFPKSMYKLKYPAPGAPHLAKRVKEPLLGSGFSHVDEDKKRGLDHGAWVPLLLMYPEADIPVCQLSISSNKGGTYHYNMGKALTPLKDEGVLIVGSGSATHNLRAIAPRGTPPAPWASAFMSWLKTFFL 236

HSP 2 Score: 31.5722 bits (70), Expect = 1.229e-87
Identity = 11/21 (52.38%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHPW 795
            RY+++N Y+EK P  K+ HPW
Sbjct:  239 RYEEVNEYEEKAPYAKMVHPW 259

[Close]

BLAST of GR398650 vs. TrEMBL
Match: C6TJ92_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 306.99 bits (785), Expect = 2.739e-84
Identity = 149/201 (74.13%), Postives = 172/201 (85.57%), Query Frame = 3

Query:  138 MALKETFYISHGSPTLAIDETIPAWKFLKQWK-EVFPHRPSAILVISGHWDTSVPTVNVVQH-NQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHRDSPPPAWALAFDSWLKNHSLK 734
            MALK+TFYISHGSPTL+IDE+I A KFL+ WK +VFP RPS+ILVISGHW+T+VPTVNVV   N TIYDF GFP+ MY+LKYPAPGAPQLAR+VKEL++ SG S VDED KRGLDHG WVPL LMYPEADIPVCQ+S+ SQ+DGTYHYNLGKALAPLKDEGVLI+GSGSA HN+RA+    +  P WAL FD+WLK+  L+
Sbjct:    1 MALKDTFYISHGSPTLSIDESIQARKFLQSWKKDVFPQRPSSILVISGHWETAVPTVNVVDSINDTIYDFYGFPKQMYQLKYPAPGAPQLARRVKELLKKSGFSHVDEDTKRGLDHGAWVPLFLMYPEADIPVCQISIQSQQDGTYHYNLGKALAPLKDEGVLIMGSGSAVHNLRALEPHSTVAP-WALEFDNWLKDALLE 200

HSP 2 Score: 31.187 bits (69), Expect = 2.739e-84
Identity = 12/21 (57.14%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHPW 795
            RY+ +NHY++K P  K AHPW
Sbjct:  202 RYEVVNHYEQKAPHAKKAHPW 222

[Close]

BLAST of GR398650 vs. TrEMBL
Match: B9NCP2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_590946 PE=4 SV=1)

HSP 1 Score: 296.975 bits (759), Expect = 1.924e-82
Identity = 137/195 (70.26%), Postives = 160/195 (82.05%), Query Frame = 3

Query:  150 ETFYISHGSPTLAIDETIPAWKFLKQWKEVFPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHRDSPPPAWALAFDSWLKNHSLK 734
            +T YISHGSP +AIDE+IPA KFLK W+++F  RP AILVISGHWDT  PTVNVV  N TIYDF GFP+SMYKLKY  PGAP LA++VKEL+ A+G  RV EDK RG+DHGTWVPLM MYPEADIPVCQLSV + RDGTYHYNLGKALAPL++EG+LI+GSG+ THN+  +    SP P+WAL FD+WLKN  L+
Sbjct:    2 DTVYISHGSPMMAIDESIPARKFLKTWQQIFKERPKAILVISGHWDTKEPTVNVVNRNDTIYDFYGFPKSMYKLKYTPPGAPLLAKRVKELLMANGFKRVHEDKTRGVDHGTWVPLMFMYPEADIPVCQLSVQTDRDGTYHYNLGKALAPLREEGILIMGSGATTHNLGTMQPSGSPVPSWALQFDTWLKNALLE 196

HSP 2 Score: 35.039 bits (79), Expect = 1.924e-82
Identity = 13/21 (61.90%), Postives = 17/21 (80.95%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHPW 795
            RY+D+NHYD + P  K+AHPW
Sbjct:  198 RYEDVNHYDSRAPYGKMAHPW 218

[Close]

BLAST of GR398650 vs. TrEMBL
Match: B9IP00_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_573569 PE=4 SV=1)

HSP 1 Score: 296.975 bits (759), Expect = 1.924e-82
Identity = 137/195 (70.26%), Postives = 160/195 (82.05%), Query Frame = 3

Query:  150 ETFYISHGSPTLAIDETIPAWKFLKQWKEVFPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHRDSPPPAWALAFDSWLKNHSLK 734
            +T YISHGSP +AIDE+IPA KFLK W+++F  RP AILVISGHWDT  PTVNVV  N TIYDF GFP+SMYKLKY  PGAP LA++VKEL+ A+G  RV EDK RG+DHGTWVPLM MYPEADIPVCQLSV + RDGTYHYNLGKALAPL++EG+LI+GSG+ THN+  +    SP P+WAL FD+WLKN  L+
Sbjct:    2 DTVYISHGSPMMAIDESIPARKFLKTWQQIFKERPKAILVISGHWDTKEPTVNVVNRNDTIYDFYGFPKSMYKLKYTPPGAPLLAKRVKELLMANGFKRVHEDKTRGVDHGTWVPLMFMYPEADIPVCQLSVQTDRDGTYHYNLGKALAPLREEGILIMGSGATTHNLGTMQPSGSPVPSWALQFDTWLKNALLE 196

HSP 2 Score: 35.039 bits (79), Expect = 1.924e-82
Identity = 13/21 (61.90%), Postives = 17/21 (80.95%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHPW 795
            RY+D+NHYD + P  K+AHPW
Sbjct:  198 RYEDVNHYDSRAPYGKMAHPW 218

[Close]

BLAST of GR398650 vs. TrEMBL
Match: B7FN83_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 298.516 bits (763), Expect = 1.612e-81
Identity = 145/197 (73.60%), Postives = 166/197 (84.26%), Query Frame = 3

Query:  138 MALKETFYISHGSPTLAIDETIPAWKFLKQWK-EVFPHRPSAILVISGHWDTSVPTVNVVQH-NQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHRDSPPPAWALAFDSWLKN 722
            MALK+TFYISHGSPTL+ID++I A KFL+ WK +VF  RP +ILVISGHWDT+VPTVNV+Q  N TI+DF GFP+ MY+LKYPAPGAP+LA++VKEL+  SG  RVDEDKKRGLDHG WVPLMLMYPEADIPVCQLSV S  DGTYHYNLGKALAPLKDEGVLI+GSGSA HN+  +  R    P WAL FD+WLK+
Sbjct:    1 MALKDTFYISHGSPTLSIDDSIEARKFLQSWKKDVFEERPKSILVISGHWDTTVPTVNVIQTTNDTIHDFYGFPKPMYQLKYPAPGAPELAKRVKELLNKSGFDRVDEDKKRGLDHGAWVPLMLMYPEADIPVCQLSVQSDLDGTYHYNLGKALAPLKDEGVLIMGSGSAVHNLGTLNPRAGVAP-WALEFDNWLKD 196

HSP 2 Score: 30.4166 bits (67), Expect = 1.612e-81
Identity = 12/20 (60.00%), Postives = 16/20 (80.00%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHP 792
            RY+D+NHY++K P  K AHP
Sbjct:  202 RYEDVNHYEQKAPHAKKAHP 221

[Close]

BLAST of GR398650 vs. TrEMBL
Match: A5BGZ7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021798 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 6.654e-80
Identity = 135/191 (70.68%), Postives = 157/191 (82.20%), Query Frame = 3

Query:  150 ETFYISHGSPTLAIDETIPAWKFLKQWKE-VFPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHRDSPPPAWALAFDSWLK 719
            +TFYISHGSPTL+IDE++PA  FLK WKE V   RP++ILVIS HW+T  PTVNVV  N TIYDF GFP+SMYKLKYPAPGAP+LA++VKEL+ AS    V EDKKRGLDHG WVPLMLMYPEADIPVCQLSV + RDG+YHYN+G+ALAPL++EGVLI+GSGSATHN+RA+         WA  FD+WLK
Sbjct:    2 DTFYISHGSPTLSIDESLPARHFLKSWKEKVMGQRPTSILVISAHWETHEPTVNVVPQNDTIYDFYGFPKSMYKLKYPAPGAPELAKRVKELLMASNFKSVKEDKKRGLDHGAWVPLMLMYPEADIPVCQLSVQTNRDGSYHYNMGRALAPLREEGVLIIGSGSATHNLRALNPNSESVVPWAYEFDTWLK 192

HSP 2 Score: 35.039 bits (79), Expect = 6.654e-80
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHPW 795
            RY+DINHY+EK P  + AHPW
Sbjct:  199 RYEDINHYEEKAPHAREAHPW 219

[Close]

BLAST of GR398650 vs. TrEMBL
Match: B9H432_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_818033 PE=4 SV=1)

HSP 1 Score: 285.419 bits (729), Expect = 1.237e-78
Identity = 133/196 (67.86%), Postives = 158/196 (80.61%), Query Frame = 3

Query:  150 ETFYISHGSPTLAIDETIPAWKFLKQWKE-VFPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHRDSPPPAWALAFDSWLKNHSLK 734
            +T YISHGSP +AIDE+IPA +FLK W++ V   RP AILVISGHWDT  PTVNVV  N TIYDF GFP+ MY+LKY  PGAPQLA++VKEL+  +G   V EDKKRG+DHGTWVPLM MYPEADIPVCQLSV S RDGTYHYN+GKALAPLK+EG+L++GSG+ THN+ A+    +P P+WA  FD+WLKN  L+
Sbjct:    2 DTVYISHGSPMMAIDESIPARQFLKSWQQTVLKERPKAILVISGHWDTKEPTVNVVNINDTIYDFYGFPKPMYQLKYTPPGAPQLAKRVKELLMENGFKHVHEDKKRGVDHGTWVPLMFMYPEADIPVCQLSVQSDRDGTYHYNMGKALAPLKEEGILVMGSGATTHNLGAMHPEGTPVPSWASQFDTWLKNALLE 197

HSP 2 Score: 33.8834 bits (76), Expect = 1.237e-78
Identity = 13/21 (61.90%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHPW 795
            RY+D+NHYD + P  K AHPW
Sbjct:  199 RYEDVNHYDSRAPYGKKAHPW 219

[Close]

BLAST of GR398650 vs. TrEMBL
Match: A9PEK6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_831223 PE=2 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 2.104e-78
Identity = 133/196 (67.86%), Postives = 163/196 (83.16%), Query Frame = 3

Query:  138 MALKETFYISHGSPTLAIDETIPAWKFLKQWKE-VFPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHRDSPPPAWALAFDSWLKN 722
            MAL ETFY+SHG+PTLAIDET+PA KF + W++  +  +PS+ILVIS HW+T+ PTVNVV  N TIYDF GFP+ +Y++KY  PGAP+LA++VKEL+ ASG+  VDEDKKRGLDHG WVPLM MYPEADIPVCQLSV S RDGT+HYN+GKALAPL++EGVLI+GSGSA HN+ +     SP P+WAL FD+WLK+
Sbjct:    1 MALMETFYLSHGAPTLAIDETVPARKFFQSWQQSAYKEKPSSILVISAHWETAQPTVNVVDRNDTIYDFYGFPKPLYQIKYTPPGAPELAKRVKELLLASGIEHVDEDKKRGLDHGAWVPLMFMYPEADIPVCQLSVQSDRDGTHHYNMGKALAPLREEGVLILGSGSAVHNLGSRLPDGSPVPSWALEFDNWLKD 196

HSP 2 Score: 29.6462 bits (65), Expect = 2.104e-78
Identity = 12/20 (60.00%), Postives = 16/20 (80.00%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHP 792
            RY+D+NHY+ K P  K+AHP
Sbjct:  202 RYEDVNHYESKAPHPKMAHP 221

[Close]

BLAST of GR398650 vs. TrEMBL
Match: B9RWA0_RICCO (4,5-DOPA dioxygenase extradiol, putative OS=Ricinus communis GN=RCOM_1176870 PE=4 SV=1)

HSP 1 Score: 281.567 bits (719), Expect = 1.762e-77
Identity = 130/198 (65.66%), Postives = 157/198 (79.29%), Query Frame = 3

Query:  144 LKETFYISHGSPTLAIDETIPAWKFLKQWKEV-FPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHRDSPPPAWALAFDSWLKNHSLK 734
            ++ETFY+SHGSP ++IDE+I A  F + W    F  RP+AIL+ISGHWDT  P VN+V  N TIYDF GFP  MYKLKY  PGAP LA++VKEL+  SG  RVDEDKKRGLDHG WVPLM MYPEADIPVCQLS+ S +D TYHYN+G+AL+PLK++GVLI+GSGS THN+RAI    +PPP+WAL FDSWL++  L+
Sbjct:    4 VRETFYLSHGSPMMSIDESIQARHFFQSWTNATFKQRPNAILMISGHWDTPHPAVNLVPLNDTIYDFYGFPDPMYKLKYTPPGAPDLAKRVKELLVESGFKRVDEDKKRGLDHGAWVPLMFMYPEADIPVCQLSIQSDKDATYHYNMGRALSPLKEDGVLIIGSGSTTHNLRAIQPHGTPPPSWALQFDSWLQDALLQ 201

HSP 2 Score: 33.8834 bits (76), Expect = 1.762e-77
Identity = 13/21 (61.90%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHPW 795
            RY+D+NH+D K P  K AHPW
Sbjct:  203 RYEDVNHFDMKAPHAKTAHPW 223

[Close]

BLAST of GR398650 vs. TrEMBL
Match: D7TB33_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_130.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00000251001 PE=4 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 4.665e-75
Identity = 132/194 (68.04%), Postives = 155/194 (79.90%), Query Frame = 3

Query:  150 ETFYISHGSPTLAIDETIPAWKFLKQWKE-VFPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELIEASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHRDSPPPA---WALAFDSWLK 719
            +TFYISHGSPTL+IDE++PA  FLK W+E V    P++ILVISGHW+T  PTVNVV  N TIYDF GFP+ MY+ KYPAPGAP+LA++VKEL+ AS    V+EDK+RGLDHG WVPLMLMYPEADIPVCQLSV + RDG+YHYN+GKALAPL++EGVLI GSGSATHN+R I        A   WA  FD+WLK
Sbjct:    2 DTFYISHGSPTLSIDESLPARHFLKSWREKVMGQPPTSILVISGHWETHEPTVNVVPRNDTIYDFYGFPKPMYEFKYPAPGAPELAKRVKELLMASDFKCVNEDKERGLDHGAWVPLMLMYPEADIPVCQLSVQTNRDGSYHYNMGKALAPLREEGVLIFGSGSATHNLRTINRNSDSKAAVVPWAYEFDTWLK 195

HSP 2 Score: 30.4166 bits (67), Expect = 4.665e-75
Identity = 12/21 (57.14%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHPW 795
            RY+D+N Y+EK P  + AHPW
Sbjct:  202 RYEDVNCYEEKAPHAREAHPW 222

[Close]

BLAST of GR398650 vs. TAIR peptide
Match: AT4G15093.1 (| Symbols: | catalytic LigB subunit of aromatic ring-opening dioxygenase family | chr4:8618454-8619472 FORWARD LENGTH=269)

HSP 1 Score: 264.618 bits (675), Expect = 1.113e-73
Identity = 125/201 (62.19%), Postives = 157/201 (78.11%), Query Frame = 3

Query:  144 LKETFYISHGSPTLAIDETIPAWKFLKQW-KEVFPHRPSAILVISGHWDTSVPTVNVVQHNQTIYDFSGFPRSMYKLKYPAPGAPQLARKVKELI-EASGLSRVDEDKKRGLDHGTWVPLMLMYPEADIPVCQLSVSSQRDGTYHYNLGKALAPLKDEGVLIVGSGSATHNMRAIGHR--DSPPPAWALAFDSWLKNHSLK 734
            + +TF++SHGSPTL+ID+++ A +F K W ++V P +P +ILVIS HWDT  P+VN V  N TI+DFSGFP  MYKLKY APGA +L ++VKEL+ +  G+ RVDED KRGLDHG WVPLMLMYPEADIP+CQLSV S ++G+YHYN+GKALA LKDEGVLI+GSGSATHN+R +     D  P  WAL FD WL++  L+
Sbjct:    4 VNQTFFLSHGSPTLSIDDSLEARQFFKSWTQKVLPQKPKSILVISAHWDTKFPSVNTVLRNNTIHDFSGFPDPMYKLKYEAPGAIELGKRVKELLMKEGGMKRVDEDTKRGLDHGAWVPLMLMYPEADIPICQLSVQSNQNGSYHYNMGKALASLKDEGVLIIGSGSATHNLRKLDFNITDGSPVPWALEFDHWLRDSLLQ 204

HSP 2 Score: 30.8018 bits (68), Expect = 1.113e-73
Identity = 12/21 (57.14%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  736 RYDDINHYDEK-PICKVAHPW 795
            RY D+N ++EK P  K+AHPW
Sbjct:  206 RYGDVNEWEEKAPNAKMAHPW 226

The following BLAST results are available for this feature:

BLAST of GR398650 vs. SwissProt
BLAST of GR398650 vs. TrEMBL
BLAST of GR398650 vs. TAIR peptide

BLAST of GR398650 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
DIOXL_ARATH	1.133e-72	62.19	4,5-DOPA dioxygenase extradiol-like protein OS=Ara...	[more]
DODA_PORGR	6.356e-55	49.50	4,5-DOPA dioxygenase extradiol OS=Portulaca grandi...	[more]
YGID_ECOLI	1.932e-36	39.06	Uncharacterized protein ygiD OS=Escherichia coli (...	[more]
DIOXL_SCHPO	1.760e-13	27.69	4,5-DOPA dioxygenase extradiol-like protein OS=Sch...	[more]

BLAST of GR398650 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TM42_SOYBN	1.229e-87	74.24	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TJ92_SOYBN	2.739e-84	74.13	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9NCP2_POPTR	1.924e-82	70.26	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9IP00_POPTR	1.924e-82	70.26	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B7FN83_MEDTR	1.612e-81	73.60	Putative uncharacterized protein OS=Medicago trunc...	[more]
A5BGZ7_VITVI	6.654e-80	70.68	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9H432_POPTR	1.237e-78	67.86	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PEK6_POPTR	2.104e-78	67.86	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RWA0_RICCO	1.762e-77	65.66	4,5-DOPA dioxygenase extradiol, putative OS=Ricinu...	[more]
D7TB33_VITVI	4.665e-75	68.04	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of GR398650 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G15093.1	1.113e-73	62.19	\| Symbols: \| catalytic LigB subunit of aromatic r...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004183	Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B	PFAM	PF02900	LigB	coord: 32..221 score: 3.4
IPR004183	Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B	SUPERFAMILY	53213	LigB subunit of an aromatic-ring-opening dioxygenase LigAB	coord: 27..219 score: 2.2
IPR014436	Extradiol aromatic ring-opening dioxygenase, DODA type	PIR	PIRSF006157	Extradiol aromatic ring-opening dioxygenase, DODA type	coord: 25..226 score: 6.8
None	No IPR available	GENE3D	3.40.830.10	no description	coord: 22..223 score: 5.8
None	No IPR available	SEG	seg	seg	coord: 39..59 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR398650 ID=GR398650; Name=GR398650; organism=Cicer arietinum; type=EST; length=800bp
AATGCAAAGTTGTTCTCACTTCTTCTCCTTTCTAATATATGTAATTAAAA
AACATATAAATGAAGATGATATCAAGATTTAATCATAAACTAAGAAACGA
AAAGAGAAAAGAAATAGTGAATTCAATAATAGAAAAGATGGCATTGAAGG
AAACGTTTTACATATCACATGGATCACCAACACTAGCCATAGATGAAACA
ATTCCTGCATGGAAATTCTTGAAGCAATGGAAGGAGGTTTTTCCTCATAG
ACCCTCTGCCATTCTTGTTATATCTGGTCACTGGGACACCTCTGTTCCAA
CCGTCAACGTTGTACAACACAATCAAACCATCTATGACTTCTCTGGCTTC
CCCAGAAGCATGTACAAGCTCAAGTATCCTGCACCAGGGGCACCACAGTT
GGCAAGGAAGGTGAAGGAACTAATTGAAGCATCCGGTTTAAGTCGAGTGG
ACGAAGATAAAAAGCGGGGCCTTGACCATGGAACATGGGTGCCTCTCATG
TTGATGTATCCAGAGGCTGATATACCAGTGTGTCAACTTTCTGTTTCATC
ACAAAGAGATGGTACTTACCATTATAACTTGGGAAAGGCATTAGCTCCTC
TTAAGGATGAAGGTGTTCTTATAGTGGGGTCAGGAAGTGCCACCCATAAC
ATGAGAGCAATTGGACATCGTGATAGCCCTCCTCCTGCTTGGGCTCTGGC
ATTTGATTCTTGGTTAAAAAATCACTCGTTGAAGAAGATATGATGACATA
AACCATTATGATGAGAAGCCCATATGCAAAGTGGCTCATCCTTGGCTGAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006725	cellular aromatic compound metabolic process
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008198	ferrous iron binding
GO:0016491	oxidoreductase activity
GO:0008270	zinc ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR004183	Xdiol_dOase_suB
IPR014436	Extradiol_dOase_DODA

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