GR406722

EST Overview


Name	GR406722
Unique Name	GR406722
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	257

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR406722 vs. TrEMBL
Match: B9T8E7_RICCO (Monoglyceride lipase, putative OS=Ricinus communis GN=RCOM_0386810 PE=4 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 5.482e-31
Identity = 62/81 (76.54%), Postives = 74/81 (91.36%), Query Frame = -2

Query:   14 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNE 256
            F+VLHGEEDRV DK+VS QL++VASS DKT+KLYPGMWHGLL+GEP EN+DIVFKDII W+E+R+  GNTRLERELKQ+N+
Sbjct:  112 FMVLHGEEDRVTDKSVSSQLFNVASSTDKTIKLYPGMWHGLLYGEPLENIDIVFKDIIEWLEKRATQGNTRLERELKQRND 192

[Close]

BLAST of GR406722 vs. TrEMBL
Match: O80628_ARATH (Putative phospholipase; alternative splicing isoform OS=Arabidopsis thaliana GN=At2g39410 PE=4 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 3.326e-28
Identity = 58/81 (71.60%), Postives = 70/81 (86.42%), Query Frame = -2

Query:   14 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNE 256
            FLVLHG++D+V DKAVS++LY VA S DKTLKLYPGMWHGLL GE PEN++IVF D+ISW+E+RS YGN R E ELKQ+N+
Sbjct:  231 FLVLHGDDDKVTDKAVSQELYKVALSADKTLKLYPGMWHGLLTGETPENIEIVFADVISWLEKRSDYGNDRFESELKQRND 311

[Close]

BLAST of GR406722 vs. TrEMBL
Match: D7MXF4_ARALY (Predicted protein (Fragment) OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_686651 PE=4 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 2.156e-27
Identity = 57/83 (68.67%), Postives = 68/83 (81.93%), Query Frame = -2

Query:    8 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNEDF 256
            F+VLHG +D+V DKAVS++LY VA S DKTLKLYPGMWHGLL GE PEN++IVF D+I W+E+RS YGN R E ELKQ N+ F
Sbjct:   84 FMVLHGGDDKVTDKAVSQELYKVALSADKTLKLYPGMWHGLLNGETPENIEIVFADVIGWLEKRSDYGNDRFESELKQNNDGF 166

[Close]

BLAST of GR406722 vs. TrEMBL
Match: D7LD42_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_669799 PE=4 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 2.156e-27
Identity = 57/83 (68.67%), Postives = 68/83 (81.93%), Query Frame = -2

Query:    8 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNEDF 256
            F+VLHG +D+V DKAVS++LY VA S DKTLKLYPGMWHGLL GE PEN++IVF D+I W+E+RS YGN R E ELKQ N+ F
Sbjct:  231 FMVLHGGDDKVTDKAVSQELYKVALSADKTLKLYPGMWHGLLNGETPENIEIVFADVIGWLEKRSDYGNDRFESELKQNNDGF 313

[Close]

BLAST of GR406722 vs. TrEMBL
Match: B9HTX2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_567499 PE=4 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 6.272e-27
Identity = 58/85 (68.24%), Postives = 70/85 (82.35%), Query Frame = -2

Query:    2 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNEDFVK 256
            F+VLHGE DRV DK+VS+QL  VASS DKT+KLYP MWHGLL+GEP EN DIVF+DII W++ R+  GNTRLERE K +++DF K
Sbjct:  231 FIVLHGEADRVTDKSVSEQLLRVASSSDKTIKLYPEMWHGLLYGEPVENSDIVFEDIIDWLDNRTDRGNTRLEREQKHKHDDFSK 315

[Close]

BLAST of GR406722 vs. TrEMBL
Match: D7TRP7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_317.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00005971001 PE=4 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 5.309e-26
Identity = 52/84 (61.90%), Postives = 68/84 (80.95%), Query Frame = -2

Query:    5 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNEDFV 256
            FLV+HG +D+V D + SKQL++ A+S DKT KLYPGMWHGL  GEPPEN+D VF DII+W++ERS  GN+RLE+E K +N+ F+
Sbjct:  228 FLVVHGGDDKVTDPSTSKQLHETAASADKTFKLYPGMWHGLTSGEPPENIDTVFSDIINWLDERSAIGNSRLEKERKSRNDGFI 311

[Close]

BLAST of GR406722 vs. TrEMBL
Match: D7LV17_ARALY (Esterase/lipase/thioesterase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_485941 PE=4 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 5.309e-26
Identity = 56/81 (69.14%), Postives = 66/81 (81.48%), Query Frame = -2

Query:   14 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNE 256
            F+VLHGE+D+V DK VSK LY+VASS DKT KLYP MWHGLL+GE PENL+IVF DIISW+ ER+   N RLE ELKQ ++
Sbjct:  226 FIVLHGEDDKVTDKNVSKLLYEVASSSDKTFKLYPNMWHGLLYGESPENLEIVFSDIISWLNERASVTNQRLETELKQMDD 306

[Close]

BLAST of GR406722 vs. TrEMBL
Match: Q9M3D1_ARATH (Lipase-like protein OS=Arabidopsis thaliana GN=At3g55180 PE=4 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.545e-25
Identity = 55/83 (66.27%), Postives = 67/83 (80.72%), Query Frame = -2

Query:    8 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNEDF 256
            F+VLHGE+D+V DK VSK LY+VASS DKT KLYP MWHGLL+GE PENL+IVF DIISW++ER+   N +LE ELK  ++ F
Sbjct:  226 FIVLHGEDDKVTDKNVSKLLYEVASSSDKTFKLYPNMWHGLLYGESPENLEIVFSDIISWLKERASVTNQKLETELKHVDDGF 308

[Close]

BLAST of GR406722 vs. TrEMBL
Match: B9HM32_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_765387 PE=4 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 2.018e-25
Identity = 55/81 (67.90%), Postives = 66/81 (81.48%), Query Frame = -2

Query:   14 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNE 256
            FLVLHGEED+V DK+VS++L+ VASS DKT+KLYP MWHGLL+GEP EN DIVF DII W+  R+  GNTRLE E K+ N+
Sbjct:  231 FLVLHGEEDKVTDKSVSEKLFSVASSSDKTIKLYPEMWHGLLYGEPVENRDIVFGDIIDWLGNRTHLGNTRLEMEQKRMND 311

[Close]

BLAST of GR406722 vs. TrEMBL
Match: D7LD44_ARALY (Esterase/lipase/thioesterase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_482961 PE=4 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 3.441e-25
Identity = 52/81 (64.20%), Postives = 68/81 (83.95%), Query Frame = -2

Query:   14 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNE 256
            F+VLHGE+D+V DKAVS+QLY+VASS DKT KLYPGMWHGLL+GE PEN++IVF DII W++++   G+   E ELK++N+
Sbjct:  231 FMVLHGEDDKVTDKAVSRQLYEVASSADKTFKLYPGMWHGLLYGETPENIEIVFADIIGWLDKKVSDGHGGFESELKRKND 311

[Close]

BLAST of GR406722 vs. TAIR peptide
Match: AT2G39410.2 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr2:16455667-16458217 FORWARD LENGTH=317)

HSP 1 Score: 127.872 bits (320), Expect = 1.294e-30
Identity = 58/81 (71.60%), Postives = 70/81 (86.42%), Query Frame = -2

Query:   14 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNE 256
            FLVLHG++D+V DKAVS++LY VA S DKTLKLYPGMWHGLL GE PEN++IVF D+ISW+E+RS YGN R E ELKQ+N+
Sbjct:  231 FLVLHGDDDKVTDKAVSQELYKVALSADKTLKLYPGMWHGLLTGETPENIEIVFADVISWLEKRSDYGNDRFESELKQRND 311

[Close]

BLAST of GR406722 vs. TAIR peptide
Match: AT3G55180.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:20454903-20456682 FORWARD LENGTH=312)

HSP 1 Score: 119.013 bits (297), Expect = 6.009e-28
Identity = 55/83 (66.27%), Postives = 67/83 (80.72%), Query Frame = -2

Query:    8 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNEDF 256
            F+VLHGE+D+V DK VSK LY+VASS DKT KLYP MWHGLL+GE PENL+IVF DIISW++ER+   N +LE ELK  ++ F
Sbjct:  226 FIVLHGEDDKVTDKNVSKLLYEVASSSDKTFKLYPNMWHGLLYGESPENLEIVFSDIISWLKERASVTNQKLETELKHVDDGF 308

[Close]

BLAST of GR406722 vs. TAIR peptide
Match: AT2G39420.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr2:16460442-16462872 FORWARD LENGTH=317)

HSP 1 Score: 112.464 bits (280), Expect = 5.624e-26
Identity = 50/81 (61.73%), Postives = 66/81 (81.48%), Query Frame = -2

Query:   14 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNE 256
            F+VLHGE+D+V DKAVS+QLY+VASS DKT KLYPGMWHGLL+GE PEN++ VF DII W++++    +   E ELK++N+
Sbjct:  231 FIVLHGEDDKVTDKAVSRQLYEVASSSDKTFKLYPGMWHGLLYGETPENIETVFADIIGWLDKKVADESGGFESELKRKND 311

[Close]

BLAST of GR406722 vs. TAIR peptide
Match: AT2G39400.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr2:16452719-16454650 FORWARD LENGTH=311)

HSP 1 Score: 110.538 bits (275), Expect = 2.137e-25
Identity = 50/85 (58.82%), Postives = 67/85 (78.82%), Query Frame = -2

Query:    2 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLERELKQQNEDFVK 256
            F+VLHGE+D+V DK++SK LY+VASS DKT KLYP MWH LL+GE  EN +IVF DII+W+E+R+   N  LE +LK +++ F+K
Sbjct:  225 FIVLHGEDDKVTDKSISKMLYEVASSSDKTFKLYPKMWHALLYGETNENSEIVFGDIINWLEDRATDSNGGLESQLKHKHDGFLK 309

[Close]

BLAST of GR406722 vs. TAIR peptide
Match: AT3G55190.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:20458007-20459890 FORWARD LENGTH=319)

HSP 1 Score: 98.2117 bits (243), Expect = 1.098e-21
Identity = 44/64 (68.75%), Postives = 52/64 (81.25%), Query Frame = -2

Query:   65 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEER 256
            F+VLHGE+D+V DK  SK LY+VA S DKTLKLYP MWH LLFGEPPEN +IVF DI+ W++ R
Sbjct:  232 FIVLHGEDDKVTDKGGSKLLYEVALSNDKTLKLYPEMWHSLLFGEPPENSEIVFNDIVQWMQTR 295

[Close]

BLAST of GR406722 vs. TAIR peptide
Match: AT3G62860.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:23239577-23242143 REVERSE LENGTH=348)

HSP 1 Score: 88.5817 bits (218), Expect = 8.697e-19
Identity = 38/65 (58.46%), Postives = 50/65 (76.92%), Query Frame = -2

Query:   62 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERS 256
            F VLHGE D V D  +SK L++ AS+ DKT+KLYPGMWHGL  GEP  N+D+VF DI++W++ R+
Sbjct:  228 FFVLHGEADIVTDPEISKALFEKASTRDKTIKLYPGMWHGLTSGEPDANVDLVFADIVNWLDART 292

[Close]

BLAST of GR406722 vs. TAIR peptide
Match: AT2G47630.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr2:19534637-19536911 FORWARD LENGTH=351)

HSP 1 Score: 88.1965 bits (217), Expect = 1.136e-18
Identity = 41/65 (63.08%), Postives = 49/65 (75.38%), Query Frame = -2

Query:   62 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERS 256
            F VLHGE D V D  VSK LY+ AS+ DKTLKLYPGMWH L  GEP  N+D+VF DII+W++ R+
Sbjct:  230 FFVLHGEADTVTDPEVSKALYEKASTRDKTLKLYPGMWHALTSGEPDCNVDLVFADIINWLDLRT 294

[Close]

BLAST of GR406722 vs. TAIR peptide
Match: AT1G11090.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr1:3702655-3703629 FORWARD LENGTH=324)

HSP 1 Score: 83.1889 bits (204), Expect = 3.654e-17
Identity = 36/73 (49.32%), Postives = 52/73 (71.23%), Query Frame = -2

Query:   38 FLVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERSRYGNTRLE 256
            F+++HG  D V D  VS++LY+ A S DKTLK+Y GM H +LFGEP +N++IV KDI+SW+ +R     T+ +
Sbjct:  251 FIIVHGSADAVTDPEVSRELYEHAKSKDKTLKIYDGMMHSMLFGEPDDNIEIVRKDIVSWLNDRCGGDKTKTQ 323

[Close]

BLAST of GR406722 vs. TAIR peptide
Match: AT5G14980.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr5:4849600-4850583 FORWARD LENGTH=327)

HSP 1 Score: 73.9442 bits (180), Expect = 2.217e-14
Identity = 33/64 (51.56%), Postives = 47/64 (73.44%), Query Frame = -2

Query:   62 LVLHGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEERS 253
            +++HG +D V D A  ++LY   SS DKT+K+YPGMWH L+ GE  EN+D+VF D++ WI+ RS
Sbjct:  254 MIVHGRDDVVCDPASVEELYRRCSSRDKTIKIYPGMWHQLI-GESEENVDLVFGDVLDWIKTRS 316

[Close]

BLAST of GR406722 vs. TAIR peptide
Match: AT1G52760.1 (| Symbols: LysoPL2 | lysophospholipase 2 | chr1:19651378-19652576 FORWARD LENGTH=332)

HSP 1 Score: 71.2478 bits (173), Expect = 1.437e-13
Identity = 35/66 (53.03%), Postives = 45/66 (68.18%), Query Frame = -2

Query:   50 HGEEDRVIDKAVSKQLYDVASSIDKTLKLYPGMWHGLLFGEPPENLDIVFKDIISWIEER-SRYGN 244
            HG  D V     SK LY+ ASS DKTLK+Y GM+H L+ GEP EN +IV KD+  WI+E+  +YG+
Sbjct:  264 HGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENAEIVLKDMREWIDEKVKKYGS 329

The following BLAST results are available for this feature:

BLAST of GR406722 vs. TrEMBL
BLAST of GR406722 vs. TAIR peptide

BLAST of GR406722 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9T8E7_RICCO	5.482e-31	76.54	Monoglyceride lipase, putative OS=Ricinus communis...	[more]
O80628_ARATH	3.326e-28	71.60	Putative phospholipase; alternative splicing isofo...	[more]
D7MXF4_ARALY	2.156e-27	68.67	Predicted protein (Fragment) OS=Arabidopsis lyrata...	[more]
D7LD42_ARALY	2.156e-27	68.67	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
B9HTX2_POPTR	6.272e-27	68.24	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TRP7_VITVI	5.309e-26	61.90	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7LV17_ARALY	5.309e-26	69.14	Esterase/lipase/thioesterase family protein OS=Ara...	[more]
Q9M3D1_ARATH	1.545e-25	66.27	Lipase-like protein OS=Arabidopsis thaliana GN=At3...	[more]
B9HM32_POPTR	2.018e-25	67.90	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7LD44_ARALY	3.441e-25	64.20	Esterase/lipase/thioesterase family protein OS=Ara...	[more]

BLAST of GR406722 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT2G39410.2	1.294e-30	71.60	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT3G55180.1	6.009e-28	66.27	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT2G39420.1	5.624e-26	61.73	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT2G39400.1	2.137e-25	58.82	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT3G55190.1	1.098e-21	68.75	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT3G62860.1	8.697e-19	58.46	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT2G47630.1	1.136e-18	63.08	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT1G11090.1	3.654e-17	49.32	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT5G14980.1	2.217e-14	51.56	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT1G52760.1	1.437e-13	53.03	\| Symbols: LysoPL2 \| lysophospholipase 2 \| chr1:19...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 38..49 score
None	No IPR available	SEG	seg	seg	coord: 49..60 score
None	No IPR available	GENE3D	3.40.50.1820	no description	coord: 1..66 score: 2
None	No IPR available	PANTHER	PTHR11614	PHOSPHOLIPASE-RELATED	coord: 1..83 score: 4.1
None	No IPR available	PANTHER	PTHR11614:SF20	LYSOPHOSPHOLIPASE	coord: 1..83 score: 4.1
None	No IPR available	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 1..64 score: 6.7

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR406722 ID=GR406722; Name=GR406722; organism=Cicer arietinum; type=EST; length=257bp
TTTTTACAAAATCTTCATTTTGTTGTTTCAGTTCTCTCTCTAATCTTGTG
TTACCATAACGAGATCTCTCCTCTATCCAACTAATAATATCTTTGAATAC
AATGTCCAAATTTTCTGGGGGCTCTCCAAACAATAAACCATGCCACATTC
CTGGGTACAACTTCAGTGTCTTATCAATGCTTGAGGCCACATCATATAAT
TGTTTGCTCACTGCTTTATCAATCACTCTATCTTCTTCACCATGTAAAAC
CAAAAAA

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