GR916603

EST Overview


Name	GR916603
Unique Name	GR916603
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	387

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR916603 vs. SwissProt
Match: SGPL_ARATH (Sphingosine-1-phosphate lyase OS=Arabidopsis thaliana GN=At1g27980 PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 1.534e-52
Identity = 68/81 (83.95%), Postives = 75/81 (92.59%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RKHQFVAV EWSGGLYVSPTIAGSRPGSL+AGAWAAMMSLG+EGYL+NT  IME SKRL++G+ EI ELF+IGKPDMTIVA
Sbjct:  368 RKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWAAMMSLGEEGYLQNTSKIMEASKRLEEGVREIHELFVIGKPDMTIVA 448

HSP 2 Score: 82.4185 bits (202), Expect = 1.534e-52
Identity = 36/41 (87.80%), Postives = 39/41 (95.12%), Query Frame = -3

Query:   23 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKHVP 145
            FGS  LDIFEVNDIMSSKGWHLNALQRPNSIHIC+T++HVP
Sbjct:  449 FGSKALDIFEVNDIMSSKGWHLNALQRPNSIHICITLQHVP 489

[Close]

BLAST of GR916603 vs. SwissProt
Match: SGPL_ORYSJ (Sphingosine-1-phosphate lyase OS=Oryza sativa subsp. japonica GN=SPL PE=2 SV=2)

HSP 1 Score: 122.865 bits (307), Expect = 3.546e-45
Identity = 59/81 (72.84%), Postives = 68/81 (83.95%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RK  F +V EW+GGLYVSPTIAGSRPG LIAGAWAAM SLG  GY+ENT  IME SK++Q+GIE+I  LF+IGKPDMT+VA
Sbjct:  363 RKPYFFSVTEWTGGLYVSPTIAGSRPGGLIAGAWAAMTSLGLNGYMENTGHIMEVSKKIQRGIEDIPGLFVIGKPDMTVVA 443

HSP 2 Score: 78.5666 bits (192), Expect = 3.546e-45
Identity = 34/39 (87.18%), Postives = 38/39 (97.44%), Query Frame = -3

Query:   29 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKH 145
            FGSD +DIFEVNDIMSSKGWHLNALQRPNS+HICVT++H
Sbjct:  444 FGSDSVDIFEVNDIMSSKGWHLNALQRPNSLHICVTLQH 482

[Close]

BLAST of GR916603 vs. SwissProt
Match: SGPL_DICDI (Sphingosine-1-phosphate lyase OS=Dictyostelium discoideum GN=sglA PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.125e-29
Identity = 38/90 (42.22%), Postives = 60/90 (66.67%), Query Frame = -1

Query:  124 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVAL--DPMFWI 387
            R+  +   P W GG+Y SPT+ GSRPG L+A  WA+++S+G +G+LE  K +ME +K++ KG++ I  + IIG P   +VA   D +F++
Sbjct:  343 RRAMYFVAPNWPGGIYASPTLPGSRPGGLVAACWASLVSMGNDGFLEKAKGVMETTKKIIKGLQSINGVKIIGDPKAMVVAFTCDNIFYV 432

HSP 2 Score: 60.4622 bits (145), Expect = 1.125e-29
Identity = 25/34 (73.53%), Postives = 29/34 (85.29%), Query Frame = -3

Query:   26 DIFEVNDIMSSKGWHLNALQRPNSIHICVTVKHV 127
            +IF VND MS KGWHLNALQRPNS+H+CVT K +
Sbjct:  428 NIFYVNDYMSKKGWHLNALQRPNSLHVCVTAKMI 461

[Close]

BLAST of GR916603 vs. SwissProt
Match: SGPL1_RAT (Sphingosine-1-phosphate lyase 1 OS=Rattus norvegicus GN=Sgpl1 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 5.878e-28
Identity = 39/82 (47.56%), Postives = 59/82 (71.95%), Query Frame = -1

Query:  142 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVAL 387
            RK+QF    +W GG+Y SP+IAGSRPG +IA  WAA+M  G+ GY+E TK I++ ++ L+  +E I  +FI+G P ++++AL
Sbjct:  372 RKYQFFVDADWQGGIYASPSIAGSRPGGIIAACWAALMHFGENGYVEATKQIIKTARFLKSELENIKNIFILGDPQLSVIAL 453

HSP 2 Score: 51.2174 bits (121), Expect = 5.878e-28
Identity = 21/44 (47.73%), Postives = 31/44 (70.45%), Query Frame = -3

Query:   11 GSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKHVPARAA 142
            GS+  DI+ ++++MS+KGW+ N LQ P SIH C+T+ H   R A
Sbjct:  454 GSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITLVHTRKRVA 497

[Close]

BLAST of GR916603 vs. SwissProt
Match: SGPL_DROME (Sphingosine-1-phosphate lyase OS=Drosophila melanogaster GN=Sply PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 3.725e-27
Identity = 38/82 (46.34%), Postives = 57/82 (69.51%), Query Frame = -1

Query:  142 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVAL 387
            + HQF    +W GG+Y SPT+ GSR G +IA  WA MMS G +GYLE TK I++ ++ +++G+ +I  +FI GKP  +++AL
Sbjct:  361 KDHQFTVTTDWPGGVYGSPTVNGSRAGGIIAACWATMMSFGYDGYLEATKRIVDTARYIERGVRDIDGIFIFGKPATSVIAL 442

HSP 2 Score: 50.0618 bits (118), Expect = 3.725e-27
Identity = 21/38 (55.26%), Postives = 28/38 (73.68%), Query Frame = -3

Query:   29 GSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKH 142
            GS+V DIF ++D +   GW+LNALQ P+ IH+CVT  H
Sbjct:  443 GSNVFDIFRLSDSLCKLGWNLNALQFPSGIHLCVTDMH 480

[Close]

BLAST of GR916603 vs. SwissProt
Match: SGPL1_MOUSE (Sphingosine-1-phosphate lyase 1 OS=Mus musculus GN=Sgpl1 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 6.307e-27
Identity = 38/82 (46.34%), Postives = 57/82 (69.51%), Query Frame = -1

Query:  142 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVAL 387
            R +QF    +W GG+Y SP+IAGSRPG +IA  WAA+M  G+ GY+E TK I++ ++ L+  +E I  +FI G P ++++AL
Sbjct:  372 RTYQFFVGADWQGGVYASPSIAGSRPGGIIAACWAALMHFGENGYVEATKQIIKTARFLKSELENIKNIFIFGDPQLSVIAL 453

HSP 2 Score: 51.2174 bits (121), Expect = 6.307e-27
Identity = 21/44 (47.73%), Postives = 31/44 (70.45%), Query Frame = -3

Query:   11 GSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKHVPARAA 142
            GS+  DI+ ++++MS+KGW+ N LQ P SIH C+T+ H   R A
Sbjct:  454 GSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITLVHTRKRVA 497

[Close]

BLAST of GR916603 vs. SwissProt
Match: SGPL1_PONAB (Sphingosine-1-phosphate lyase 1 OS=Pongo abelii GN=SGPL1 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.810e-26
Identity = 37/82 (45.12%), Postives = 56/82 (68.29%), Query Frame = -1

Query:  142 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVAL 387
            R +QF    +W GG+Y SPTIAGSRPG + A  WAA+M  G+ GY+E TK I++ ++ L+  +E I  +F+ G P ++++AL
Sbjct:  372 RNYQFFVDTDWQGGIYASPTIAGSRPGGISAACWAALMHFGENGYVEATKQIIKTARFLKSELENIKGIFVFGNPQLSVIAL 453

HSP 2 Score: 50.447 bits (119), Expect = 1.810e-26
Identity = 21/44 (47.73%), Postives = 31/44 (70.45%), Query Frame = -3

Query:   11 GSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKHVPARAA 142
            GS   DI+ ++++M++KGW+LN LQ P SIH C+T+ H   R A
Sbjct:  454 GSRDFDIYRLSNLMTAKGWNLNQLQFPPSIHFCITLLHARKRVA 497

[Close]

BLAST of GR916603 vs. SwissProt
Match: SGPL1_HUMAN (Sphingosine-1-phosphate lyase 1 OS=Homo sapiens GN=SGPL1 PE=1 SV=3)

HSP 1 Score: 88.1965 bits (217), Expect = 1.810e-26
Identity = 37/82 (45.12%), Postives = 56/82 (68.29%), Query Frame = -1

Query:  142 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVAL 387
            R +QF    +W GG+Y SPTIAGSRPG + A  WAA+M  G+ GY+E TK I++ ++ L+  +E I  +F+ G P ++++AL
Sbjct:  372 RNYQFFVDTDWQGGIYASPTIAGSRPGGISAACWAALMHFGENGYVEATKQIIKTARFLKSELENIKGIFVFGNPQLSVIAL 453

HSP 2 Score: 50.447 bits (119), Expect = 1.810e-26
Identity = 21/44 (47.73%), Postives = 31/44 (70.45%), Query Frame = -3

Query:   11 GSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKHVPARAA 142
            GS   DI+ ++++M++KGW+LN LQ P SIH C+T+ H   R A
Sbjct:  454 GSRDFDIYRLSNLMTAKGWNLNQLQFPPSIHFCITLLHARKRVA 497

[Close]

BLAST of GR916603 vs. SwissProt
Match: SGPL_YEAST (Sphingosine-1-phosphate lyase OS=Saccharomyces cerevisiae GN=DPL1 PE=1 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 5.949e-24
Identity = 35/82 (42.68%), Postives = 59/82 (71.95%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEE-IAELFIIGKPDMTIVA 387
            R HQ+   P W+GGLY SPT+AGSRPG+++ G WA M+++G+ GY+E+ + I+  + + +K I+E I +L I+G P  ++++
Sbjct:  399 RMHQYYVNPAWTGGLYGSPTLAGSRPGAIVVGCWATMVNMGENGYIESCQEIVGAAMKFKKYIQENIPDLNIMGNPRYSVIS 480

HSP 2 Score: 47.3654 bits (111), Expect = 5.949e-24
Identity = 20/42 (47.62%), Postives = 28/42 (66.67%), Query Frame = -3

Query:   38 RYDNSGFGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVT 163
            RY    F S  L+I E++D +S KGWH NALQ+P ++H+  T
Sbjct:  475 RYSVISFSSKTLNIHELSDRLSKKGWHFNALQKPVALHMAFT 516

[Close]

BLAST of GR916603 vs. SwissProt
Match: SGPL_CAEEL (Sphingosine-1-phosphate lyase OS=Caenorhabditis elegans GN=spl-1 PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 8.199e-19
Identity = 31/78 (39.74%), Postives = 52/78 (66.67%), Query Frame = -1

Query:  145 QFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 378
            Q+ +V +W GG+Y +PTIAGSR G+  A AWA ++S G++ Y+     I++ ++ L + IE+I  +   GK D+++VA
Sbjct:  369 QYFSVADWCGGIYATPTIAGSRAGANTAVAWATLLSFGRDEYVRRCAQIVKHTRMLAEKIEKIKWIKPYGKSDVSLVA 446

HSP 2 Score: 45.0542 bits (105), Expect = 8.199e-19
Identity = 18/37 (48.65%), Postives = 27/37 (72.97%), Query Frame = -3

Query:   35 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTV 145
            F  + ++I+EV+D M   GW+LN LQ P +IHIC+T+
Sbjct:  447 FSGNGVNIYEVSDKMMKLGWNLNTLQNPAAIHICLTI 483

[Close]

BLAST of GR916603 vs. TrEMBL
Match: Q549V9_ARATH (Sphingosine-1-phosphate lyase OS=Arabidopsis thaliana GN=AtSPL1 PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 3.126e-51
Identity = 68/81 (83.95%), Postives = 75/81 (92.59%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RKHQFVAV EWSGGLYVSPTIAGSRPGSL+AGAWAAMMSLG+EGYL+NT  IME SKRL++G+ EI ELF+IGKPDMTIVA
Sbjct:  368 RKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWAAMMSLGEEGYLQNTSKIMEASKRLEEGVREIHELFVIGKPDMTIVA 448

HSP 2 Score: 82.4185 bits (202), Expect = 3.126e-51
Identity = 36/41 (87.80%), Postives = 39/41 (95.12%), Query Frame = -3

Query:   23 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKHVP 145
            FGS  LDIFEVNDIMSSKGWHLNALQRPNSIHIC+T++HVP
Sbjct:  449 FGSKALDIFEVNDIMSSKGWHLNALQRPNSIHICITLQHVP 489

[Close]

BLAST of GR916603 vs. TrEMBL
Match: D7KBV9_ARALY (Pyridoxal-dependent decarboxylase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_473000 PE=4 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 3.126e-51
Identity = 68/81 (83.95%), Postives = 75/81 (92.59%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RKHQFVAV EWSGGLYVSPTIAGSRPGSL+AGAWAAMMSLG+EGYL+NT  IME SKRL++G+ EI ELF+IGKPDMTIVA
Sbjct:  368 RKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWAAMMSLGEEGYLQNTSKIMEASKRLEEGVREIHELFVIGKPDMTIVA 448

HSP 2 Score: 82.4185 bits (202), Expect = 3.126e-51
Identity = 36/41 (87.80%), Postives = 39/41 (95.12%), Query Frame = -3

Query:   23 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKHVP 145
            FGS  LDIFEVNDIMSSKGWHLNALQRPNSIHIC+T++HVP
Sbjct:  449 FGSKALDIFEVNDIMSSKGWHLNALQRPNSIHICITLQHVP 489

[Close]

BLAST of GR916603 vs. TrEMBL
Match: B9GZM5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1074404 PE=3 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 4.075e-51
Identity = 71/81 (87.65%), Postives = 76/81 (93.83%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RKHQFVAV EWSGGLYVSPTIAGSRPG LIAGAWAA+M+LG EGYLENTKAIME SKR+QKGI+EI ELFIIG+PDMTIVA
Sbjct:  364 RKHQFVAVTEWSGGLYVSPTIAGSRPGGLIAGAWAALMALGLEGYLENTKAIMEVSKRIQKGIKEIPELFIIGRPDMTIVA 444

HSP 2 Score: 80.4925 bits (197), Expect = 4.075e-51
Identity = 35/41 (85.37%), Postives = 39/41 (95.12%), Query Frame = -3

Query:   23 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKHVP 145
            FGS+ LDIFEVNDIMSSKGWHLNALQRPNSIHIC+T++H P
Sbjct:  445 FGSNDLDIFEVNDIMSSKGWHLNALQRPNSIHICITLQHAP 485

[Close]

BLAST of GR916603 vs. TrEMBL
Match: B9RMB8_RICCO (Sphingosine phosphate lyase, putative OS=Ricinus communis GN=RCOM_1079290 PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 9.805e-50
Identity = 68/81 (83.95%), Postives = 75/81 (92.59%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RKHQFVAV EWSGGLYVSPTIAGSRPG LIAGAWAAMMSLG EGYL+NTK IME +K++QKGIEE+ ELF+IG+PDMTIVA
Sbjct:  365 RKHQFVAVTEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGLEGYLKNTKVIMEVTKKIQKGIEEMPELFVIGRPDMTIVA 445

HSP 2 Score: 78.9518 bits (193), Expect = 9.805e-50
Identity = 34/39 (87.18%), Postives = 39/39 (100.00%), Query Frame = -3

Query:   29 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKH 145
            FGS+VLDIFEVNDI+SSKGWHLNALQRPNS+HICVT++H
Sbjct:  446 FGSNVLDIFEVNDILSSKGWHLNALQRPNSLHICVTLQH 484

[Close]

BLAST of GR916603 vs. TrEMBL
Match: D7TB09_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_10.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00010526001 PE=4 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 2.358e-48
Identity = 65/81 (80.25%), Postives = 75/81 (92.59%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RKHQFVAV EWSGGLYVSPTIAGSRPG+LIAGAWAAM+SLG+EGYL NT+  ME SK+LQ+GI++I ELF+IG+PDMTIVA
Sbjct:  353 RKHQFVAVTEWSGGLYVSPTIAGSRPGALIAGAWAAMISLGQEGYLNNTRETMEVSKKLQRGIKDIPELFVIGRPDMTIVA 433

HSP 2 Score: 78.9518 bits (193), Expect = 2.358e-48
Identity = 34/40 (85.00%), Postives = 40/40 (100.00%), Query Frame = -3

Query:   26 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKHV 145
            FGS+V+DIFEVNDI+SSKGWHLNALQRPNSIHIC+T++HV
Sbjct:  434 FGSNVVDIFEVNDILSSKGWHLNALQRPNSIHICLTLQHV 473

[Close]

BLAST of GR916603 vs. TrEMBL
Match: C5XFN1_SORBI (Putative uncharacterized protein Sb03g009170 OS=Sorghum bicolor GN=Sb03g009170 PE=3 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 8.870e-48
Identity = 63/81 (77.78%), Postives = 72/81 (88.89%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RKHQFVAV EW+GGLYVSPT+AGSRPG LIAGAWAAMMSLG  GYL+NT+ +ME SK++Q+GI EI  LF+IGKPDMTIVA
Sbjct:  357 RKHQFVAVTEWTGGLYVSPTMAGSRPGGLIAGAWAAMMSLGLNGYLDNTRCVMEVSKKIQRGIGEIPGLFVIGKPDMTIVA 437

HSP 2 Score: 80.8777 bits (198), Expect = 8.870e-48
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = -3

Query:   29 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKH 145
            FGSDV+DIFEVNDIMSSKGWHLNALQRPNS+HICVT++H
Sbjct:  438 FGSDVVDIFEVNDIMSSKGWHLNALQRPNSLHICVTLQH 476

[Close]

BLAST of GR916603 vs. TrEMBL
Match: B6SZI2_MAIZE (Sphingosine-1-phosphate lyase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 7.386e-47
Identity = 62/81 (76.54%), Postives = 71/81 (87.65%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RKHQFVAV EW+GGLYVSPTIAGSRPG LIAGAWAAMMSLG  GYL++T  IM+ SK++Q+GI EI  LF+IGKPDMT+VA
Sbjct:  357 RKHQFVAVTEWTGGLYVSPTIAGSRPGGLIAGAWAAMMSLGLNGYLDSTSRIMDVSKKIQRGIGEIPGLFVIGKPDMTVVA 437

HSP 2 Score: 80.8777 bits (198), Expect = 7.386e-47
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = -3

Query:   29 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKH 145
            FGSDV+DIFEVNDIMSSKGWHLNALQRPNS+HICVT++H
Sbjct:  438 FGSDVVDIFEVNDIMSSKGWHLNALQRPNSLHICVTLQH 476

[Close]

BLAST of GR916603 vs. TrEMBL
Match: B8ACR6_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_00010 PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 1.255e-46
Identity = 63/81 (77.78%), Postives = 71/81 (87.65%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RKHQFVAV EW+GGLYVSPTIAGSRPG LIAGAWAAM SLG  GY+ENT  IME SK++Q+GIE+I  LF+IGKPDMT+VA
Sbjct:  363 RKHQFVAVTEWTGGLYVSPTIAGSRPGGLIAGAWAAMTSLGLNGYMENTGHIMEVSKKIQRGIEDIPGLFVIGKPDMTVVA 443

HSP 2 Score: 78.5666 bits (192), Expect = 1.255e-46
Identity = 34/39 (87.18%), Postives = 38/39 (97.44%), Query Frame = -3

Query:   29 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKH 145
            FGSD +DIFEVNDIMSSKGWHLNALQRPNS+HICVT++H
Sbjct:  444 FGSDSVDIFEVNDIMSSKGWHLNALQRPNSLHICVTLQH 482

[Close]

BLAST of GR916603 vs. TrEMBL
Match: B7FAK6_ORYSJ (Os01g0100900 protein OS=Oryza sativa subsp. japonica GN=Os01g0100900 PE=2 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 1.255e-46
Identity = 63/81 (77.78%), Postives = 71/81 (87.65%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RKHQFVAV EW+GGLYVSPTIAGSRPG LIAGAWAAM SLG  GY+ENT  IME SK++Q+GIE+I  LF+IGKPDMT+VA
Sbjct:  363 RKHQFVAVTEWTGGLYVSPTIAGSRPGGLIAGAWAAMTSLGLNGYMENTGHIMEVSKKIQRGIEDIPGLFVIGKPDMTVVA 443

HSP 2 Score: 78.5666 bits (192), Expect = 1.255e-46
Identity = 34/39 (87.18%), Postives = 38/39 (97.44%), Query Frame = -3

Query:   29 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKH 145
            FGSD +DIFEVNDIMSSKGWHLNALQRPNS+HICVT++H
Sbjct:  444 FGSDSVDIFEVNDIMSSKGWHLNALQRPNSLHICVTLQH 482

[Close]

BLAST of GR916603 vs. TrEMBL
Match: A9NUF1_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 5.547e-44
Identity = 58/81 (71.60%), Postives = 72/81 (88.89%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RK QFVAV EWSGGLYVSPT+AGSRPG LIAGAWAAMM++G+EGYLE  + +ME SK +Q+GI++I ELFI+G+PDMT++A
Sbjct:  368 RKQQFVAVTEWSGGLYVSPTVAGSRPGGLIAGAWAAMMTVGEEGYLEAVRKVMEVSKTIQQGIKQIPELFIVGRPDMTVIA 448

HSP 2 Score: 73.559 bits (179), Expect = 5.547e-44
Identity = 30/39 (76.92%), Postives = 38/39 (97.44%), Query Frame = -3

Query:   29 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKH 145
            FGSD LDIF+VND++S++GWHLNALQRPNSIHIC+T++H
Sbjct:  449 FGSDKLDIFKVNDVLSTQGWHLNALQRPNSIHICLTLQH 487

[Close]

BLAST of GR916603 vs. TAIR peptide
Match: AT1G27980.1 (| Symbols: DPL1, ATDPL1 | dihydrosphingosine phosphate lyase | chr1:9748812-9752618 FORWARD LENGTH=544)

HSP 1 Score: 143.665 bits (361), Expect = 1.525e-53
Identity = 68/81 (83.95%), Postives = 75/81 (92.59%), Query Frame = -1

Query:  145 RKHQFVAVPEWSGGLYVSPTIAGSRPGSLIAGAWAAMMSLGKEGYLENTKAIMEGSKRLQKGIEEIAELFIIGKPDMTIVA 387
            RKHQFVAV EWSGGLYVSPTIAGSRPGSL+AGAWAAMMSLG+EGYL+NT  IME SKRL++G+ EI ELF+IGKPDMTIVA
Sbjct:  368 RKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWAAMMSLGEEGYLQNTSKIMEASKRLEEGVREIHELFVIGKPDMTIVA 448

HSP 2 Score: 82.4185 bits (202), Expect = 1.525e-53
Identity = 36/41 (87.80%), Postives = 39/41 (95.12%), Query Frame = -3

Query:   23 FGSDVLDIFEVNDIMSSKGWHLNALQRPNSIHICVTVKHVP 145
            FGS  LDIFEVNDIMSSKGWHLNALQRPNSIHIC+T++HVP
Sbjct:  449 FGSKALDIFEVNDIMSSKGWHLNALQRPNSIHICITLQHVP 489

The following BLAST results are available for this feature:

BLAST of GR916603 vs. SwissProt
BLAST of GR916603 vs. TrEMBL
BLAST of GR916603 vs. TAIR peptide

BLAST of GR916603 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SGPL_ARATH	1.534e-52	83.95	Sphingosine-1-phosphate lyase OS=Arabidopsis thali...	[more]
SGPL_ORYSJ	3.546e-45	72.84	Sphingosine-1-phosphate lyase OS=Oryza sativa subs...	[more]
SGPL_DICDI	1.125e-29	42.22	Sphingosine-1-phosphate lyase OS=Dictyostelium dis...	[more]
SGPL1_RAT	5.878e-28	47.56	Sphingosine-1-phosphate lyase 1 OS=Rattus norvegic...	[more]
SGPL_DROME	3.725e-27	46.34	Sphingosine-1-phosphate lyase OS=Drosophila melano...	[more]
SGPL1_MOUSE	6.307e-27	46.34	Sphingosine-1-phosphate lyase 1 OS=Mus musculus GN...	[more]
SGPL1_PONAB	1.810e-26	45.12	Sphingosine-1-phosphate lyase 1 OS=Pongo abelii GN...	[more]
SGPL1_HUMAN	1.810e-26	45.12	Sphingosine-1-phosphate lyase 1 OS=Homo sapiens GN...	[more]
SGPL_YEAST	5.949e-24	42.68	Sphingosine-1-phosphate lyase OS=Saccharomyces cer...	[more]
SGPL_CAEEL	8.199e-19	39.74	Sphingosine-1-phosphate lyase OS=Caenorhabditis el...	[more]

BLAST of GR916603 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q549V9_ARATH	3.126e-51	83.95	Sphingosine-1-phosphate lyase OS=Arabidopsis thali...	[more]
D7KBV9_ARALY	3.126e-51	83.95	Pyridoxal-dependent decarboxylase family protein O...	[more]
B9GZM5_POPTR	4.075e-51	87.65	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RMB8_RICCO	9.805e-50	83.95	Sphingosine phosphate lyase, putative OS=Ricinus c...	[more]
D7TB09_VITVI	2.358e-48	80.25	Whole genome shotgun sequence of line PN40024, sca...	[more]
C5XFN1_SORBI	8.870e-48	77.78	Putative uncharacterized protein Sb03g009170 OS=So...	[more]
B6SZI2_MAIZE	7.386e-47	76.54	Sphingosine-1-phosphate lyase OS=Zea mays PE=2 SV=...	[more]
B8ACR6_ORYSI	1.255e-46	77.78	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B7FAK6_ORYSJ	1.255e-46	77.78	Os01g0100900 protein OS=Oryza sativa subsp. japoni...	[more]
A9NUF1_PICSI	5.547e-44	71.60	Putative uncharacterized protein OS=Picea sitchens...	[more]

BLAST of GR916603 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G27980.1	1.525e-53	83.95	\| Symbols: DPL1, ATDPL1 \| dihydrosphingosine phosp...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002129	Pyridoxal phosphate-dependent decarboxylase	PANTHER	PTHR11999	GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE	coord: 1..83 score: 1.8
IPR002129	Pyridoxal phosphate-dependent decarboxylase	PANTHER	PTHR11999	GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE	coord: 15..58 score: 7.3
IPR015424	Pyridoxal phosphate-dependent transferase, major domain	SUPERFAMILY	53383	PLP-dependent transferases	coord: 1..82 score: 5.4
None	No IPR available	PANTHER	PTHR11999:SF5	SPHINGOSINE PHOSPHATE LYASE	coord: 1..83 score: 1.8
None	No IPR available	PANTHER	PTHR11999:SF5	SPHINGOSINE PHOSPHATE LYASE	coord: 15..58 score: 7.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR916603 ID=GR916603; Name=GR916603; organism=Cicer arietinum; type=EST; length=387bp
GATTTCGAGCGGCCGCCCGGGCAGGTACATGCTTCACCGTCACACAGATA
TGGATGCTGTTGGGTCTCTGCAATGCATTCAAATGCCACCCTTTGGATGA
CATGATATCGTTCACTTCAAATATATCCAGAACATCGGATCCAAAGCCAC
TATTGTCATATCGGGTTTTCCAATGATAAACAACTCAGCAATCTCCTCAA
TGCCTTTTTGTAGTCTTTTTGATCCTTCCATAATTGCTTTTGTATTTTCC
AAGTAACCTTCTTTCCCTAGAGACATCATTGCTGCCCATGCACCAGCAAT
AAGGGATCCAGGTCTGCTTCCAGCTATGGTTGGAGATACATACAATCCAC
CAGACCACTCGGGAACGGCAACAAATTGATGCTTTCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016831	carboxy-lyase activity
GO:0030170	pyridoxal phosphate binding

Vocabulary: **Biological Process**
Term	Definition
GO:0019752	carboxylic acid metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR002129	PyrdxlP-dep_de-COase
IPR015424	PyrdxlP-dep_Trfase

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