CK148861

EST Overview
NameCK148861
Unique NameCK148861
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length546
Libraries
Library NameType
LIBEST_014674 Suppressive subtractive hybridization librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of CK148861 vs. TrEMBL
Match: D7TKB9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_3.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00021542001 PE=4 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.959e-40
Identity = 71/132 (53.79%), Postives = 85/132 (64.39%), Query Frame = 2 
Query:  149 SSRVQGSKATSLSYVGSQPPWTSMSTSASGSAFGSPRSSCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQKVGQILVRTKLRASHVVHT*VLHHCYLGQYPEAQDARESFARQLDSGHKANCAWKGNS 544
            SS   GSKA SLS +GSQ P TS+STSA GSA GS R+SCRPWERGDLLRRLATF P NWFGKP++ +SLACAQ+    +   K+                G   E   A E+F ++LD+ HK NC W+GNS
Sbjct:   38 SSHGHGSKAASLSCIGSQQPRTSLSTSAGGSALGSSRTSCRPWERGDLLRRLATFKPSNWFGKPKVASSLACAQRGWINVDVDKIMCESCGAYLSFVSLPSGTPAEVDSAGEAFGKELDTEHKVNCPWRGNS 169

HSP 2 Score: 38.891 bits (89), Expect = 2.959e-40
Identity = 18/20 (90.00%), Postives = 19/20 (95.00%), Query Frame = 3 
Query:   36 MREEVISSGGTVDPTTAASS 95
            MREEVISSGGT+DPT AASS
Sbjct:    1 MREEVISSGGTIDPTPAASS 20

HSP 3 Score: 35.4242 bits (80), Expect = 2.959e-40
Identity = 17/23 (73.91%), Postives = 17/23 (73.91%), Query Frame = 1 
Query:   76 PQPLPVLAGASSPAVPMNVGSID 144
            P P    AGASSPAVP NVGSID
Sbjct:   14 PTPAASSAGASSPAVPTNVGSID 36
[Close]
BLAST of CK148861 vs. TrEMBL
Match: A5AZQ1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_032620 PE=4 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 3.830e-35
Identity = 71/132 (53.79%), Postives = 85/132 (64.39%), Query Frame = 2 
Query:  149 SSRVQGSKATSLSYVGSQPPWTSMSTSASGSAFGSPRSSCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQKVGQILVRTKLRASHVVHT*VLHHCYLGQYPEAQDARESFARQLDSGHKANCAWKGNS 544
            SS   GSKA SLS +GSQ P TS+STSA GSA GS R+SCRPWERGDLLRRLATF P NWFGKP++ +SLACAQ+    +   K+                G   E   A E+F ++LD+ HK NC W+GNS
Sbjct:   39 SSHGHGSKAASLSCIGSQQPRTSLSTSAGGSALGSSRTSCRPWERGDLLRRLATFKPSNWFGKPKVASSLACAQRGWINVDVDKIMCESCGAYLSFVSLPSGTPAEVDSAGEAFGKELDTEHKVNCPWRGNS 170

HSP 2 Score: 36.5798 bits (83), Expect = 3.830e-35
Identity = 19/32 (59.38%), Postives = 21/32 (65.62%), Query Frame = 1 
Query:   49 LSAPVAPLIPQPLPVLAGASSPAVPMNVGSID 144
            +S+  A   P P    AGASSPAVP NVGSID
Sbjct:    6 MSSGDAKFDPSPAASSAGASSPAVPTNVGSID 37
[Close]
BLAST of CK148861 vs. TrEMBL
Match: B9HZP7_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_660537 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 5.069e-28
Identity = 63/120 (52.50%), Postives = 76/120 (63.33%), Query Frame = 2 
Query:  212 TSMSTSASGSAFGSP-RSSCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQK------VGQILVRTKLRASHVVHT*VLHHCYLGQY--PEAQDARESFARQLDSGHKANCAWKGNS 544
            TS+STSA GSA GS  R SCRPWERGDLLRRLATF P NWF KP+I +SLACAQ+      + QI   T    +H+          L  +   E + A E+FA+QLD GH+A C W+GNS
Sbjct:   48 TSLSTSAGGSALGSSSRPSCRPWERGDLLRRLATFKPPNWFAKPKIASSLACAQRGWTNIDINQIACET--CGAHMTFV------SLSSWTPAEVESAAEAFAKQLDVGHQATCPWRGNS 159

HSP 2 Score: 38.1206 bits (87), Expect = 5.069e-28
Identity = 18/20 (90.00%), Postives = 19/20 (95.00%), Query Frame = 3 
Query:   36 MREEVISSGGTVDPTTAASS 95
            MREEVISSGGT+DPT AASS
Sbjct:    4 MREEVISSGGTMDPTPAASS 23
[Close]
BLAST of CK148861 vs. TrEMBL
Match: B9T408_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0485200 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 7.053e-23
Identity = 57/111 (51.35%), Postives = 73/111 (65.77%), Query Frame = 2 
Query:  218 MSTSASGSAFGSPRSSCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQK--VGQILVRTKLRASHVVHT*VLHHCYLGQYPEAQDARESFARQLDSGHKANCAWKGNS 544
            MSTSA GS  GS + SCRPWERGDLLRRLATF P NWFGKP+I +SLACA++  +   + +    +     + VL   +     E + A E+FA+QLD GHK +C W+GNS
Sbjct:    1 MSTSAGGSVLGSSKPSCRPWERGDLLRRLATFKPSNWFGKPKIASSLACARRGWMNTDVDKVVCESCSACLSFVLLPSW--TQAEVESAGEAFAKQLDDGHKVSCPWRGNS 109
[Close]
BLAST of CK148861 vs. TrEMBL
Match: B9F6C4_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_12854 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 6.969e-19
Identity = 52/138 (37.68%), Postives = 73/138 (52.90%), Query Frame = 2 
Query:  131 LAALM*SSRVQGSKATSLSYVGSQPPWTSMSTSASGSAFGSPRSSCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQKVGQILVRTKLRASHVVHT*VLHHCYLGQYPEAQDARESFARQLDSGHKANCAWKGNS 544
            +A++  S   Q S+  S S+V       S S  A+G+A  S   SCRPWERGDLLRRLAT+ P  W  +P+  +SLACA++    +   K+         +          E  +A E+FA QLD+ H  +C W+GNS
Sbjct:   35 VASIDWSGSRQASRVDSSSHVAPHAHQPSHSFDATGTALDSA-PSCRPWERGDLLRRLATYKPTTWASRPKAASSLACARRGWVNVDMDKIECESCGAHLIFSTLTSWSPAEVSNAGEAFAEQLDASHHNSCPWRGNS 171

HSP 2 Score: 28.1054 bits (61), Expect = 6.969e-19
Identity = 15/25 (60.00%), Postives = 16/25 (64.00%), Query Frame = 1 
Query:   76 PQPLPVL--AGASSPAVPMNVGSID 144
            P P PV   AG SSPA+  NV SID
Sbjct:   15 PHPTPVASSAGPSSPAMQANVASID 39
[Close]
BLAST of CK148861 vs. TrEMBL
Match: Q10CF1_ORYSJ (Expressed protein OS=Oryza sativa subsp. japonica GN=LOC_Os03g57160 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 9.064e-19
Identity = 52/138 (37.68%), Postives = 73/138 (52.90%), Query Frame = 2 
Query:  131 LAALM*SSRVQGSKATSLSYVGSQPPWTSMSTSASGSAFGSPRSSCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQKVGQILVRTKLRASHVVHT*VLHHCYLGQYPEAQDARESFARQLDSGHKANCAWKGNS 544
            +A++  S   Q S+  S S+V       S S  A+G+A  S   SCRPWERGDLLRRLAT+ P  W  +P+  +SLACA++    +   K+         +          E  +A E+FA QLD+ H  +C W+GNS
Sbjct:   35 VASIDWSGSRQASRVDSSSHVAPHAHQPSHSFDATGTALDSA-PSCRPWERGDLLRRLATYKPTTWASRPKAASSLACARRGWVNVDMDKIECESCGAHLIFSTLTSWSPAEVSNAGEAFAEQLDASHHNSCPWRGNS 171

HSP 2 Score: 27.7202 bits (60), Expect = 9.064e-19
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 1 
Query:   76 PQPLPVLAGASSPAVPMNVGSID 144
            P P+   AG SSPA+  NV SID
Sbjct:   17 PTPVASSAGPSSPAMQANVASID 39
[Close]
BLAST of CK148861 vs. TrEMBL
Match: B8AKX3_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_13792 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 9.064e-19
Identity = 52/138 (37.68%), Postives = 73/138 (52.90%), Query Frame = 2 
Query:  131 LAALM*SSRVQGSKATSLSYVGSQPPWTSMSTSASGSAFGSPRSSCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQKVGQILVRTKLRASHVVHT*VLHHCYLGQYPEAQDARESFARQLDSGHKANCAWKGNS 544
            +A++  S   Q S+  S S+V       S S  A+G+A  S   SCRPWERGDLLRRLAT+ P  W  +P+  +SLACA++    +   K+         +          E  +A E+FA QLD+ H  +C W+GNS
Sbjct:   35 VASIDWSGSRQASRVDSSSHVAPHAHQPSHSFDATGTALDSA-PSCRPWERGDLLRRLATYKPTTWASRPKAASSLACARRGWVNVDMDKIECESCGAHLIFSTLTSWSPAEVSNAGEAFAEQLDASHHNSCPWRGNS 171

HSP 2 Score: 27.7202 bits (60), Expect = 9.064e-19
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 1 
Query:   76 PQPLPVLAGASSPAVPMNVGSID 144
            P P+   AG SSPA+  NV SID
Sbjct:   17 PTPVASSAGPSSPAMQANVASID 39
[Close]
BLAST of CK148861 vs. TrEMBL
Match: Q6ZLP6_ORYSJ (Os07g0150500 protein OS=Oryza sativa subsp. japonica GN=OJ1027_G06.28 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.376e-18
Identity = 53/127 (41.73%), Postives = 72/127 (56.69%), Query Frame = 2 
Query:  167 SKATSLSYVGSQPPWTSMSTSASGSAFGSPRSSCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQKVGQILVRTKLRASHVVHT*VLHHCYLGQYP-EAQDARESFARQLDSGHKANCAWKGNS 544
            SK  S S +    P  S+ST+A+G+A    + SCRPWERGDLLRRLATF    W  KP+  +SLACA++ G + +     A       ++        P E  +A E+FA QLD+ H  +C W+GNS
Sbjct:   54 SKVESSSQIAPHAPRPSLSTNAAGAAVDFSQPSCRPWERGDLLRRLATFKSSTWASKPKAASSLACARR-GWVNIEMDKIACESCGAHLIFTALTSWSPAEVANAGEAFAEQLDASHLGDCPWRGNS 179
[Close]
BLAST of CK148861 vs. TrEMBL
Match: A2YI76_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_24913 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.376e-18
Identity = 53/127 (41.73%), Postives = 72/127 (56.69%), Query Frame = 2 
Query:  167 SKATSLSYVGSQPPWTSMSTSASGSAFGSPRSSCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQKVGQILVRTKLRASHVVHT*VLHHCYLGQYP-EAQDARESFARQLDSGHKANCAWKGNS 544
            SK  S S +    P  S+ST+A+G+A    + SCRPWERGDLLRRLATF    W  KP+  +SLACA++ G + +     A       ++        P E  +A E+FA QLD+ H  +C W+GNS
Sbjct:   54 SKVESSSQIAPHAPRPSLSTNAAGAAVDFSQPSCRPWERGDLLRRLATFKSSTWASKPKAASSLACARR-GWVNIEMDKIACESCGAHLIFTALTSWSPAEVANAGEAFAEQLDASHLGDCPWRGNS 179
[Close]
BLAST of CK148861 vs. TrEMBL
Match: D7KFC8_ARALY (Zinc ion binding protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_471924 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 5.783e-17
Identity = 49/111 (44.14%), Postives = 63/111 (56.76%), Query Frame = 2 
Query:  221 STSASGSAFGSPRSSCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQKVGQILVRTKLRASHVVHT*VLHHCYLG---QYPEAQDARESFARQLDSGHKANCAWKGNS 544
            S   SG        SCR W+RGDLLRRLATF P NW GKP+  +SLACAQK    +   KL+  +     +LH+        +PEA   RE F++QLD  H+++C W G S
Sbjct:   66 SVDWSGHGLALSVRSCRTWDRGDLLRRLATFKPSNWLGKPKTASSLACAQKGWVSVDLDKLQCEYCGS--ILHYSPPQDSLNHPEADTTREEFSKQLDDAHESSCPWVGKS 174
[Close]
BLAST of CK148861 vs. TAIR peptide
Match: AT1G17210.1 (| Symbols: ATILP1, ILP1 | IAP-like protein 1 | chr1:5880502-5884657 REVERSE LENGTH=958)

HSP 1 Score: 82.4185 bits (202), Expect = 1.669e-16
Identity = 45/103 (43.69%), Postives = 57/103 (55.34%), Query Frame = 2 
Query:  263 SCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQKVGQILVRTKLRASHVVHT*VLHHCYLGQY---------PEAQDARESFARQLDSGHKANCAWKGNS 544
            SCR W+RGDLLRRLATF P NW GKP+  +SLACAQK    +   KL+  +           + QY         PEA    E F++QLD  H+++C W G S
Sbjct:   80 SCRTWDRGDLLRRLATFKPSNWLGKPKTASSLACAQKGWVSVDLDKLQCEYCGS--------ILQYSPPQDSLNPPEADTTGEKFSKQLDDAHESSCPWVGKS 174
[Close]
BLAST of CK148861 vs. TAIR peptide
Match: AT1G48950.1 (| Symbols: | C3HC zinc finger-like | chr1:18107063-18110108 FORWARD LENGTH=594)

HSP 1 Score: 70.0922 bits (170), Expect = 8.570e-13
Identity = 39/103 (37.86%), Postives = 55/103 (53.40%), Query Frame = 2 
Query:  251 SPRSSCRPWERGDLLRRLATFAPVNWFGKPQIINSLACAQKVGQILVRTKLRASHVVHT*VLHHCYLG-----QYPEAQDARESFARQLDSGHKANCAWKGNS 544
            SP   CRPW+RGDL+RRLATF  + WF KPQ+I+++ CA++ G +       A  +       H Y          + + A   F+ +L+SGHK  C W  NS
Sbjct:   74 SPSGLCRPWDRGDLMRRLATFKSMTWFAKPQVISAVNCARR-GWV----NDDADSIACESCGAHLYFSAPSSWSKQQVEKAASVFSLKLESGHKLLCPWIENS 171
The following BLAST results are available for this feature:
BLAST of CK148861 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
D7TKB9_VITVI2.959e-4053.79Whole genome shotgun sequence of line PN40024, sca... [more]
A5AZQ1_VITVI3.830e-3553.79Putative uncharacterized protein OS=Vitis vinifera... [more]
B9HZP7_POPTR5.069e-2852.50Predicted protein (Fragment) OS=Populus trichocarp... [more]
B9T408_RICCO7.053e-2351.35Putative uncharacterized protein OS=Ricinus commun... [more]
B9F6C4_ORYSJ6.969e-1937.68Putative uncharacterized protein OS=Oryza sativa s... [more]
Q10CF1_ORYSJ9.064e-1937.68Expressed protein OS=Oryza sativa subsp. japonica ... [more]
B8AKX3_ORYSI9.064e-1937.68Putative uncharacterized protein OS=Oryza sativa s... [more]
Q6ZLP6_ORYSJ1.376e-1841.73Os07g0150500 protein OS=Oryza sativa subsp. japoni... [more]
A2YI76_ORYSI1.376e-1841.73Putative uncharacterized protein OS=Oryza sativa s... [more]
D7KFC8_ARALY5.783e-1744.14Zinc ion binding protein OS=Arabidopsis lyrata sub... [more]
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BLAST of CK148861 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT1G17210.11.669e-1643.69| Symbols: ATILP1, ILP1 | IAP-like protein 1 | chr... [more]
AT1G48950.18.570e-1337.86| Symbols: | C3HC zinc finger-like | chr1:181070... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012935Zinc finger, C3HC-likePFAMPF07967zf-C3HCcoord: 29..56
score: 2.3
IPR012935Zinc finger, C3HC-likePFAMPF07967zf-C3HCcoord: 42..75
score: 1.1

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>CK148861 ID=CK148861; Name=CK148861; organism=Cicer arietinum; type=EST; length=546bp
CGCAGGCCGAGGTGTTAAGACAGAGTGTGTGATAGATGAGGGAAGAGGTT
ATCAGCTCCGGTGGCACCGTTGATCCCACAACCGCTGCCAGTTCTCGCTG
GGGCATCTTCCCCGGCTGTTCCGATGAATGTTGGCAGCATTGATGTGATC
AAGTCGTGTGCAAGGCTCTAAAGCGACTTCTCTATCATATGTTGGTTCAC
AACCACCATGGACTTCTATGAGCACAAGTGCTAGTGGTTCTGCATTTGGT
TCACCAAGATCTTCATGTAGGCCCTGGGAAAGAGGAGATTTATTAAGGCG
TTTGGCTACTTTTGCTCCTGTAAATTGGTTTGGGAAGCCTCAGATCATCA
ATTCATTGGCTTGTGCTCAAAAGGTTGGACAAATATTGGTGAGGACAAAA
TTGCGTGCGAGTCATGTGGTGCATACCTGAGTTTTACATCATTGTTATCT
TGGACAATATCCTGAAGCTCAAGATGCCAGGGAATCCTTTGCCAGACAGC
TGGATTCGGGGCATAAAGCCAATTGCGCTTGGAAAGGAAACAGTTG
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
Vocabulary: INTERPRO
TermDefinition
IPR012935Znf_C3HC-like