CK148872

EST Overview


Name	CK148872
Unique Name	CK148872
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	621

Libraries


Library Name	Type
LIBEST_014674 Suppressive subtractive hybridization library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of CK148872 vs. SwissProt
Match: AAK1_HUMAN (AP2-associated protein kinase 1 OS=Homo sapiens GN=AAK1 PE=1 SV=3)

HSP 1 Score: 91.6633 bits (226), Expect = 3.332e-18
Identity = 44/86 (51.16%), Postives = 61/86 (70.93%), Query Frame = 2

Query:    5 AYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQAS 262
            +YRAPEM +L+  ++I  K DIWALGCLL+++CYF   F GES++ + +GN+ IPD  ++S  +  LIR ML+  PD RPDI Q S
Sbjct:  224 SYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPF-GESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVS 308

[Close]

BLAST of CK148872 vs. SwissProt
Match: Y6461_DICDI (Probable serine/threonine-protein kinase DDB_G0276461 OS=Dictyostelium discoideum GN=DDB_G0276461 PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 9.696e-18
Identity = 43/81 (53.09%), Postives = 59/81 (72.84%), Query Frame = 2

Query:    8 YRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDI 250
            YRAPEM DL+  ++I+EKVD+WALGCLLF++ ++   FDG S LQ++N NY+IPD  K+S+    LI+ +L A P  RP I
Sbjct:  234 YRAPEMVDLYRGQIIDEKVDVWALGCLLFKMAFYVDPFDGGS-LQIINNNYKIPDNSKYSNNFHKLIQFILVADPTQRPSI 313

[Close]

BLAST of CK148872 vs. SwissProt
Match: AAK1_MOUSE (AP2-associated protein kinase 1 OS=Mus musculus GN=Aak1 PE=1 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 1.266e-17
Identity = 43/86 (50.00%), Postives = 61/86 (70.93%), Query Frame = 2

Query:    5 AYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQAS 262
            +YRAPEM +L+  ++I  K DIWALGCLL+++CYF   F GES++ + +G++ IPD  ++S  +  LIR ML+  PD RPDI Q S
Sbjct:  224 SYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPF-GESQVAICDGSFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVS 308

[Close]

BLAST of CK148872 vs. SwissProt
Match: BMP2K_HUMAN (BMP-2-inducible protein kinase OS=Homo sapiens GN=BMP2K PE=1 SV=2)

HSP 1 Score: 86.2705 bits (212), Expect = 1.400e-16
Identity = 47/103 (45.63%), Postives = 66/103 (64.08%), Query Frame = 2

Query:    5 AYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQAS------ALLDWPFISIN 295
            +YRAPEM +L+  + I  K DIWALGCLL+++C+F   F GES++ + +GN+ IPD  ++S  +  LIR ML+  P+ RPDI Q S      A  D P  +IN
Sbjct:  228 SYRAPEMINLYGGKPITTKADIWALGCLLYKLCFFTLPF-GESQVAICDGNFTIPDNSRYSRNIHCLIRFMLEPDPEHRPDIFQVSYFAFKFAKKDCPVSNIN 329

[Close]

BLAST of CK148872 vs. SwissProt
Match: AAK1_RAT (AP2-associated protein kinase 1 OS=Rattus norvegicus GN=Aak1 PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 9.075e-16
Identity = 42/86 (48.84%), Postives = 60/86 (69.77%), Query Frame = 2

Query:    5 AYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQAS 262
            +YRAPEM +L+  ++I  K DIWALGCLL+++CYF   F GES++ + +G++ IPD  ++S  +  LI  ML+  PD RPDI Q S
Sbjct:  224 SYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPF-GESQVAICDGSFTIPDNSRYSQDMHCLIY-MLEPDPDKRPDIYQVS 307

[Close]

BLAST of CK148872 vs. SwissProt
Match: BMP2K_MOUSE (BMP-2-inducible protein kinase OS=Mus musculus GN=Bmp2k PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.185e-15
Identity = 46/103 (44.66%), Postives = 66/103 (64.08%), Query Frame = 2

Query:    5 AYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQAS------ALLDWPFISIN 295
            +YRAPEM +L+  + I  K DIWALGCLL+++C+F   F GES++ + +G++ IPD  ++S  +  LIR ML+  P+ RPDI Q S      A  D P  +IN
Sbjct:  225 SYRAPEMINLYGGKPITTKADIWALGCLLYKLCFFTLPF-GESQVAICDGSFTIPDNSRYSHNVHCLIRFMLEPDPECRPDIFQVSYFAFKFAKKDCPVSNIN 326

[Close]

BLAST of CK148872 vs. SwissProt
Match: Y0111_DICDI (Probable serine/threonine-protein kinase DDB_G0280111 OS=Dictyostelium discoideum GN=DDB_G0280111 PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 5.882e-15
Identity = 39/89 (43.82%), Postives = 57/89 (64.04%), Query Frame = 2

Query:    8 YRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQASALLD 274
            YRAPE+ D +   VINEK+DIWALGCLL+++ ++ + F+    L +LN NY IP     S+ L  LI+ ML   P +RP+I + +  L+
Sbjct:  198 YRAPEIVDFYRSPVINEKIDIWALGCLLYKLLFYVTPFEDSGSLGILNSNYTIPPNHTHSNDLISLIKIMLNPDPINRPNIFEITNQLN 286

[Close]

BLAST of CK148872 vs. SwissProt
Match: PRK1_YEAST (Actin-regulating kinase PRK1 OS=Saccharomyces cerevisiae GN=PRK1 PE=1 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.310e-14
Identity = 37/83 (44.58%), Postives = 53/83 (63.86%), Query Frame = 2

Query:    8 YRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            YR+PEM DL+    I+EK DIWALG  L++ICY+ + F+   +  +L+  Y+ P  P++S  L +LIR ML   P  RP+I Q
Sbjct:  208 YRSPEMIDLYRGLPIDEKSDIWALGVFLYKICYYTTPFEKSGEAGILHARYQYPSFPQYSDRLKNLIRLMLMEAPSQRPNICQ 290

[Close]

BLAST of CK148872 vs. SwissProt
Match: GAK_RAT (Cyclin-G-associated kinase OS=Rattus norvegicus GN=Gak PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 8.494e-14
Identity = 36/85 (42.35%), Postives = 53/85 (62.35%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            P YR PE+ DL+    I EK DIWALGC+L+ +C+ +  F+  +KL+++NG Y IP      +   DLIR ML+  P++R  I +
Sbjct:  224 PMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRGMLKVNPEERLSIAE 308

[Close]

BLAST of CK148872 vs. SwissProt
Match: GAK_HUMAN (Cyclin-G-associated kinase OS=Homo sapiens GN=GAK PE=1 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 1.109e-13
Identity = 36/85 (42.35%), Postives = 52/85 (61.18%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            P YR PE+ DL+    I EK DIWALGC+L+ +C+ +  F+  +KL+++NG Y IP      +    LIR MLQ  P++R  I +
Sbjct:  224 PMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAE 308

[Close]

BLAST of CK148872 vs. TrEMBL
Match: D7LFM3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_482227 PE=4 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 6.306e-59
Identity = 70/85 (82.35%), Postives = 81/85 (95.29%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            P YRAPEMWDLF RE+I+EKVDIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S+F+TDLI++MLQA PD+RPDITQ
Sbjct:  205 PTYRAPEMWDLFRREMISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSAFVTDLIKEMLQASPDERPDITQ 289

HSP 2 Score: 95.1301 bits (235), Expect = 6.306e-59
Identity = 50/106 (47.17%), Postives = 66/106 (62.26%), Query Frame = 3

Query:  300 VWFRVNEQLPIDLQKSLPDRPPELPSSNIHEGASMSTNKSPAMXXXXXXXXXSVGESKTTPQPSLAPRGGESGGQLGAFWSTLHAKDSPVPEE-KGKPIYDEERSS 614
            +WFRVNEQLP +LQKSLPDRPPE+ S+ +H+G+S S NKS            S    ++ P P  +    +SGG LGAFW+T HAK S + E+ K  P +DE  S+
Sbjct:  290 IWFRVNEQLPANLQKSLPDRPPEMQSTGVHDGSSKSANKS------------SPVPRRSPPPPPPSSGESDSGGPLGAFWATQHAKTSVLSEDNKSMPKFDEPNSN 383

[Close]

BLAST of CK148872 vs. TrEMBL
Match: Q8H0Z0_ARATH (Putative serine/threonine protein kinase OS=Arabidopsis thaliana GN=At2g32850 PE=2 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 2.376e-58
Identity = 70/85 (82.35%), Postives = 80/85 (94.12%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            P YRAPEMWDLF RE+I+EKVDIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S F+TDLI++MLQA PD+RPDITQ
Sbjct:  205 PTYRAPEMWDLFRREMISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQ 289

HSP 2 Score: 93.9745 bits (232), Expect = 2.376e-58
Identity = 53/107 (49.53%), Postives = 65/107 (60.75%), Query Frame = 3

Query:  300 VWFRVNEQLPIDLQKSLPDRPPELPSSNIHEGASMSTNK-SPAMXXXXXXXXXSVGESKTTPQPSLAPRGGESGGQLGAFWSTLHAKDSPVPEE-KGKPIYDEERSS 614
            +WFRVNEQLP +LQKSLPDRPPE+ S+ +H+G+S S  K SPA          S GES             +SGG LGAFW+T HAK S V E+ K  P +DE  S+
Sbjct:  290 IWFRVNEQLPANLQKSLPDRPPEMQSTGVHDGSSKSATKPSPAPRRSPPPPPPSSGES-------------DSGGPLGAFWATQHAKTSVVSEDNKNMPKFDEPNSN 383

[Close]

BLAST of CK148872 vs. TrEMBL
Match: O50071_ARATH (Putative serine threonine protein kinase OS=Arabidopsis thaliana GN=At2g32850 PE=2 SV=2)

HSP 1 Score: 156.762 bits (395), Expect = 2.376e-58
Identity = 70/85 (82.35%), Postives = 80/85 (94.12%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            P YRAPEMWDLF RE+I+EKVDIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S F+TDLI++MLQA PD+RPDITQ
Sbjct:  205 PTYRAPEMWDLFRREMISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQ 289

HSP 2 Score: 93.9745 bits (232), Expect = 2.376e-58
Identity = 53/107 (49.53%), Postives = 65/107 (60.75%), Query Frame = 3

Query:  300 VWFRVNEQLPIDLQKSLPDRPPELPSSNIHEGASMSTNK-SPAMXXXXXXXXXSVGESKTTPQPSLAPRGGESGGQLGAFWSTLHAKDSPVPEE-KGKPIYDEERSS 614
            +WFRVNEQLP +LQKSLPDRPPE+ S+ +H+G+S S  K SPA          S GES             +SGG LGAFW+T HAK S V E+ K  P +DE  S+
Sbjct:  290 IWFRVNEQLPANLQKSLPDRPPEMQSTGVHDGSSKSATKPSPAPRRSPPPPPPSSGES-------------DSGGPLGAFWATQHAKTSVVSEDNKNMPKFDEPNSN 383

[Close]

BLAST of CK148872 vs. TrEMBL
Match: Q8VYC0_ARATH (Putative serine/threonine protein kinase OS=Arabidopsis thaliana GN=At2g32850 PE=2 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 3.098e-58
Identity = 69/85 (81.18%), Postives = 80/85 (94.12%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            P YRAPEMWDLF RE+I+EKVDIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S F+TDLI++MLQA PD+RPD+TQ
Sbjct:  205 PTYRAPEMWDLFRREMISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDVTQ 289

HSP 2 Score: 93.9745 bits (232), Expect = 3.098e-58
Identity = 53/107 (49.53%), Postives = 65/107 (60.75%), Query Frame = 3

Query:  300 VWFRVNEQLPIDLQKSLPDRPPELPSSNIHEGASMSTNK-SPAMXXXXXXXXXSVGESKTTPQPSLAPRGGESGGQLGAFWSTLHAKDSPVPEE-KGKPIYDEERSS 614
            +WFRVNEQLP +LQKSLPDRPPE+ S+ +H+G+S S  K SPA          S GES             +SGG LGAFW+T HAK S V E+ K  P +DE  S+
Sbjct:  290 IWFRVNEQLPANLQKSLPDRPPEMQSTGVHDGSSKSATKPSPAPRRSPPPPPPSSGES-------------DSGGPLGAFWATQHAKTSVVSEDNKNMPKFDEPNSN 383

[Close]

BLAST of CK148872 vs. TrEMBL
Match: B9REI2_RICCO (Serine/threonine protein kinase, putative OS=Ricinus communis GN=RCOM_1771100 PE=4 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 1.168e-57
Identity = 73/85 (85.88%), Postives = 79/85 (92.94%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            PAYRAPEMWDL  R+ I+EKVDIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIPDLPK+S  +TDLIRDMLQA PDDRPDITQ
Sbjct:  204 PAYRAPEMWDLLRRDRISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPDLPKYSMPVTDLIRDMLQASPDDRPDITQ 288

HSP 2 Score: 90.8929 bits (224), Expect = 1.168e-57
Identity = 50/104 (48.08%), Postives = 63/104 (60.58%), Query Frame = 3

Query:  300 VWFRVNEQLPIDLQKSLPDRPPELPSSNIHEGASMSTNKSPAMXXXXXXXXXSVGESKTTPQPSLAPRGGESGGQLGAFWSTLHAKDSPVPEEKGKPIYDEERS 611
            VWFRVNEQLP++LQKSLPD  PE+ S+++HE  S   N+ P M                +P P  +  G   GGQLGAFWS+ HA+DS V EEK K  +D+E S
Sbjct:  289 VWFRVNEQLPVNLQKSLPDGLPEMKSTSVHEVTSRPANRPPPM-------------PNRSPPPPPSSGGVGGGGQLGAFWSSQHARDSFV-EEKSKSKFDDESS 378

[Close]

BLAST of CK148872 vs. TrEMBL
Match: Q53UK1_LOTJA (Ser/Thr protein kinase OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 5.342e-53
Identity = 98/104 (94.23%), Postives = 104/104 (100.00%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQASALLDWPFISINLGLVSC 313
            PAYRAPEMWDLFL+EVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPD+PK+SS++TDLIRDMLQA+PDDRPDITQASALLDWPFISINLGLVSC
Sbjct:  204 PAYRAPEMWDLFLKEVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDVPKYSSYVTDLIRDMLQAKPDDRPDITQASALLDWPFISINLGLVSC 307

[Close]

BLAST of CK148872 vs. TrEMBL
Match: D7TNX0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_156.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001401001 PE=4 SV=1)

HSP 1 Score: 195.667 bits (496), Expect = 2.322e-48
Identity = 91/104 (87.50%), Postives = 99/104 (95.19%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQASALLDWPFISINLGLVSC 313
            PAYRAPEMWDL  RE+INEKVDIWALGCLLFRICYFKSAFDGESKLQ+LNGNYRIP+LPK+SS +TDLIRDMLQA PD+RPDITQASALLDWPFIS+ LG+VSC
Sbjct:  294 PAYRAPEMWDLLRRELINEKVDIWALGCLLFRICYFKSAFDGESKLQILNGNYRIPELPKYSSNVTDLIRDMLQASPDNRPDITQASALLDWPFISMTLGVVSC 397

[Close]

BLAST of CK148872 vs. TrEMBL
Match: A5AJP3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_036304 PE=4 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 2.839e-46
Identity = 88/100 (88.00%), Postives = 95/100 (95.00%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQASALLDWPFISINLG 301
            PAYRAPEMWDL  RE+INEKVDIWALGCLLFRICYFKSAFDGESKLQ+LNGNYRIP+LPK+SS +TDLIRDMLQA PD+RPDITQASALLDWPFIS+ LG
Sbjct:  204 PAYRAPEMWDLLRRELINEKVDIWALGCLLFRICYFKSAFDGESKLQILNGNYRIPELPKYSSNVTDLIRDMLQASPDNRPDITQASALLDWPFISMTLG 303

[Close]

BLAST of CK148872 vs. TrEMBL
Match: Q69L76_ORYSJ (Cyclin G-associated kinase-like protein OS=Oryza sativa subsp. japonica GN=OSJNBa0016G10.6-1 PE=2 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 2.425e-45
Identity = 68/85 (80.00%), Postives = 80/85 (94.12%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            PAYRAPEMWDL+ REVI+EKVDIWALGCLL+RICYFKSAFDGESKLQ+LNGNYRIP+ PK+S+ +T LI+DML+A P+DRPD+TQ
Sbjct:  204 PAYRAPEMWDLYRREVISEKVDIWALGCLLYRICYFKSAFDGESKLQILNGNYRIPEQPKYSAAVTKLIKDMLEASPNDRPDVTQ 288

HSP 2 Score: 54.299 bits (129), Expect = 2.425e-45
Identity = 38/104 (36.54%), Postives = 53/104 (50.96%), Query Frame = 3

Query:  300 VWFRVNEQLPIDLQKSLPDRPPELPSSNIH-EGASMSTNKSPAMXXXXXXXXXSVGESKTTPQPSLAPRGGESG-GQLGAFWSTLHAKDSPVPEEKGKPIYDEE 605
            VWFRVNE LP++LQK+LPD      S ++  EGA   T+  P           S          SL+    ++G   LGAFW+T HA+ S V + +   ++DEE
Sbjct:  289 VWFRVNELLPLELQKNLPDGASSGISMSLQDEGAYKRTHVMPRRNPPPPPIEQS--------NSSLSHGSSKAGDAPLGAFWATQHAQGSQVADNRNS-LFDEE 383

[Close]

BLAST of CK148872 vs. TrEMBL
Match: B8AEP9_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_07872 PE=4 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 2.425e-45
Identity = 68/85 (80.00%), Postives = 80/85 (94.12%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            PAYRAPEMWDL+ REVI+EKVDIWALGCLL+RICYFKSAFDGESKLQ+LNGNYRIP+ PK+S+ +T LI+DML+A P+DRPD+TQ
Sbjct:  204 PAYRAPEMWDLYRREVISEKVDIWALGCLLYRICYFKSAFDGESKLQILNGNYRIPEQPKYSAAVTKLIKDMLEASPNDRPDVTQ 288

HSP 2 Score: 54.299 bits (129), Expect = 2.425e-45
Identity = 38/104 (36.54%), Postives = 53/104 (50.96%), Query Frame = 3

Query:  300 VWFRVNEQLPIDLQKSLPDRPPELPSSNIH-EGASMSTNKSPAMXXXXXXXXXSVGESKTTPQPSLAPRGGESG-GQLGAFWSTLHAKDSPVPEEKGKPIYDEE 605
            VWFRVNE LP++LQK+LPD      S ++  EGA   T+  P           S          SL+    ++G   LGAFW+T HA+ S V + +   ++DEE
Sbjct:  289 VWFRVNELLPLELQKNLPDGASSGISMSLQDEGAYKRTHVMPRRNPPPPPIEQS--------NSSLSHGSSKAGDAPLGAFWATQHAQGSQVADNRNS-LFDEE 383

[Close]

BLAST of CK148872 vs. TAIR peptide
Match: AT2G32850.2 (| Symbols: | Protein kinase superfamily protein | chr2:13935448-13937977 REVERSE LENGTH=670)

HSP 1 Score: 156.762 bits (395), Expect = 1.733e-60
Identity = 70/85 (82.35%), Postives = 80/85 (94.12%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            P YRAPEMWDLF RE+I+EKVDIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S F+TDLI++MLQA PD+RPDITQ
Sbjct:  205 PTYRAPEMWDLFRREMISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQ 289

HSP 2 Score: 93.9745 bits (232), Expect = 1.733e-60
Identity = 53/107 (49.53%), Postives = 65/107 (60.75%), Query Frame = 3

Query:  300 VWFRVNEQLPIDLQKSLPDRPPELPSSNIHEGASMSTNK-SPAMXXXXXXXXXSVGESKTTPQPSLAPRGGESGGQLGAFWSTLHAKDSPVPEE-KGKPIYDEERSS 614
            +WFRVNEQLP +LQKSLPDRPPE+ S+ +H+G+S S  K SPA          S GES             +SGG LGAFW+T HAK S V E+ K  P +DE  S+
Sbjct:  290 IWFRVNEQLPANLQKSLPDRPPEMQSTGVHDGSSKSATKPSPAPRRSPPPPPPSSGES-------------DSGGPLGAFWATQHAKTSVVSEDNKNMPKFDEPNSN 383

[Close]

BLAST of CK148872 vs. TAIR peptide
Match: AT2G32850.1 (| Symbols: | Protein kinase superfamily protein | chr2:13935448-13937977 REVERSE LENGTH=650)

HSP 1 Score: 156.762 bits (395), Expect = 1.733e-60
Identity = 70/85 (82.35%), Postives = 80/85 (94.12%), Query Frame = 2

Query:    2 PAYRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFDGESKLQVLNGNYRIPDLPKFSSFLTDLIRDMLQARPDDRPDITQ 256
            P YRAPEMWDLF RE+I+EKVDIWALGCLLFRICYFK+AFDGESKLQ+LNGNYRIP+ PK+S F+TDLI++MLQA PD+RPDITQ
Sbjct:  205 PTYRAPEMWDLFRREMISEKVDIWALGCLLFRICYFKNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQ 289

HSP 2 Score: 93.9745 bits (232), Expect = 1.733e-60
Identity = 53/107 (49.53%), Postives = 65/107 (60.75%), Query Frame = 3

Query:  300 VWFRVNEQLPIDLQKSLPDRPPELPSSNIHEGASMSTNK-SPAMXXXXXXXXXSVGESKTTPQPSLAPRGGESGGQLGAFWSTLHAKDSPVPEE-KGKPIYDEERSS 614
            +WFRVNEQLP +LQKSLPDRPPE+ S+ +H+G+S S  K SPA          S GES             +SGG LGAFW+T HAK S V E+ K  P +DE  S+
Sbjct:  290 IWFRVNEQLPANLQKSLPDRPPEMQSTGVHDGSSKSATKPSPAPRRSPPPPPPSSGES-------------DSGGPLGAFWATQHAKTSVVSEDNKNMPKFDEPNSN 383

[Close]

BLAST of CK148872 vs. TAIR peptide
Match: AT5G08160.2 (| Symbols: ATPK3, PK3 | serine/threonine protein kinase 3 | chr5:2625903-2627942 REVERSE LENGTH=311)

HSP 1 Score: 51.2174 bits (121), Expect = 5.175e-7
Identity = 31/91 (34.07%), Postives = 47/91 (51.65%), Query Frame = 2

Query:    8 YRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFD-------GESKLQVLNGNYRIPDLPKFSSF---LTDLIRDMLQARPDDRPDI 250
            +RAPE+WD      I+E+ DIW+LGC L+ I Y  S F+       G  +L ++N   + P+    +S+   L   +  MLQ +   RP I
Sbjct:  205 FRAPELWDCPSHADIDERTDIWSLGCTLYAIMYGVSPFEYALGESGGSLQLAIVNAQIKWPNTGPKASYPEALHQFVTWMLQPQAAVRPRI 295

[Close]

BLAST of CK148872 vs. TAIR peptide
Match: AT5G08160.1 (| Symbols: ATPK3, PK3 | serine/threonine protein kinase 3 | chr5:2625903-2627942 REVERSE LENGTH=347)

HSP 1 Score: 51.2174 bits (121), Expect = 5.175e-7
Identity = 31/91 (34.07%), Postives = 47/91 (51.65%), Query Frame = 2

Query:    8 YRAPEMWDLFLREVINEKVDIWALGCLLFRICYFKSAFD-------GESKLQVLNGNYRIPDLPKFSSF---LTDLIRDMLQARPDDRPDI 250
            +RAPE+WD      I+E+ DIW+LGC L+ I Y  S F+       G  +L ++N   + P+    +S+   L   +  MLQ +   RP I
Sbjct:  241 FRAPELWDCPSHADIDERTDIWSLGCTLYAIMYGVSPFEYALGESGGSLQLAIVNAQIKWPNTGPKASYPEALHQFVTWMLQPQAAVRPRI 331

The following BLAST results are available for this feature:

BLAST of CK148872 vs. SwissProt
BLAST of CK148872 vs. TrEMBL
BLAST of CK148872 vs. TAIR peptide

BLAST of CK148872 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AAK1_HUMAN	3.332e-18	51.16	AP2-associated protein kinase 1 OS=Homo sapiens GN...	[more]
Y6461_DICDI	9.696e-18	53.09	Probable serine/threonine-protein kinase DDB_G0276...	[more]
AAK1_MOUSE	1.266e-17	50.00	AP2-associated protein kinase 1 OS=Mus musculus GN...	[more]
BMP2K_HUMAN	1.400e-16	45.63	BMP-2-inducible protein kinase OS=Homo sapiens GN=...	[more]
AAK1_RAT	9.075e-16	48.84	AP2-associated protein kinase 1 OS=Rattus norvegic...	[more]
BMP2K_MOUSE	1.185e-15	44.66	BMP-2-inducible protein kinase OS=Mus musculus GN=...	[more]
Y0111_DICDI	5.882e-15	43.82	Probable serine/threonine-protein kinase DDB_G0280...	[more]
PRK1_YEAST	1.310e-14	44.58	Actin-regulating kinase PRK1 OS=Saccharomyces cere...	[more]
GAK_RAT	8.494e-14	42.35	Cyclin-G-associated kinase OS=Rattus norvegicus GN...	[more]
GAK_HUMAN	1.109e-13	42.35	Cyclin-G-associated kinase OS=Homo sapiens GN=GAK ...	[more]

BLAST of CK148872 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7LFM3_ARALY	6.306e-59	82.35	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q8H0Z0_ARATH	2.376e-58	82.35	Putative serine/threonine protein kinase OS=Arabid...	[more]
O50071_ARATH	2.376e-58	82.35	Putative serine threonine protein kinase OS=Arabid...	[more]
Q8VYC0_ARATH	3.098e-58	81.18	Putative serine/threonine protein kinase OS=Arabid...	[more]
B9REI2_RICCO	1.168e-57	85.88	Serine/threonine protein kinase, putative OS=Ricin...	[more]
Q53UK1_LOTJA	5.342e-53	94.23	Ser/Thr protein kinase OS=Lotus japonicus PE=2 SV=...	[more]
D7TNX0_VITVI	2.322e-48	87.50	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AJP3_VITVI	2.839e-46	88.00	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q69L76_ORYSJ	2.425e-45	80.00	Cyclin G-associated kinase-like protein OS=Oryza s...	[more]
B8AEP9_ORYSI	2.425e-45	80.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of CK148872 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT2G32850.2	1.733e-60	82.35	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT2G32850.1	1.733e-60	82.35	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT5G08160.2	5.175e-7	34.07	\| Symbols: ATPK3, PK3 \| serine/threonine protein k...	[more]
AT5G08160.1	5.175e-7	34.07	\| Symbols: ATPK3, PK3 \| serine/threonine protein k...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 58..80 score
None	No IPR available	GENE3D	1.10.510.10	no description	coord: 1..87 score: 2.4
None	No IPR available	PANTHER	PTHR22967	SERINE/THREONINE PROTEIN KINASE	coord: 1..85 score: 6.8
None	No IPR available	PANTHER	PTHR22967:SF29	SERINE/THREONINE KINASE 16 (STK16 )	coord: 1..85 score: 6.8
None	No IPR available	PANTHER	PTHR22967	SERINE/THREONINE PROTEIN KINASE	coord: 1..94 score: 4.3
None	No IPR available	PANTHER	PTHR22967:SF29	SERINE/THREONINE KINASE 16 (STK16 )	coord: 1..94 score: 4.3
None	No IPR available	SEG	seg	seg	coord: 44..52 score
IPR000719	Protein kinase, catalytic domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 1..95 score: 14
IPR011009	Protein kinase-like domain	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 1..88 score: 6.3
IPR017442	Serine/threonine-protein kinase-like domain	PFAM	PF00069	Pkinase	coord: 2..87 score: 2.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK148872 ID=CK148872; Name=CK148872; organism=Cicer arietinum; type=EST; length=621bp
CCCTGCCTACAGAGCCCCTGAGATGTGGGATCTGTTCCTGAGAGAAGTCA
TTAACGAAAAAGTGGACATATGGGCACTTGGGTGTCTCCTTTTTCGCATA
TGCTACTTCAAAAGTGCATTTGATGGGGAATCGAAGCTCCAAGTCTTAAA
TGGAAACTACCGCATTCCTGATTTACCTAAGTTCAGTTCATTCCTCACAG
ACTTGATCAGAGACATGCTTCAAGCTAGACCAGATGACAGACCAGATATC
ACGCAGGCAAGCGCTTTGCTTGATTGGCCATTCATCTCCATCAATTTAGG
TTTGGTTTCGTGTTAATGAGCAGCTACCCATTGATTTACAAAAGTCTTTA
CCTGACAGGCCACCTGAATTGCCCTCTTCCAACATTCACGAAGGTGCTTC
AATGTCCACAAACAAATCCCCTGCAATGCCTCGCAGGAATCCACCTCCTC
CTCCTTCAGTTGGAGAATCCAAAACTACCCCTCAGCCATCTCTTGCTCCT
AGGGGAGGGGAAAGTGGGGGGCAGCTTGGTGCTTTCTGGTCCACTCTGCA
TGCAAAGGATTCACCTGTCCCCGAGGAAAAGGGAAAACCTATATATGATG
AAGAACGATCTAGCCACCATG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR011009	Kinase-like_dom_sf
IPR017442	Serine/threonine-protein kinase-like domain

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