FL512401

EST Overview
NameFL512401
Unique NameFL512401
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length326
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of FL512401 vs. SwissProt
Match: AMYA_VIGMU (Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 2.999e-23
Identity = 49/58 (84.48%), Postives = 55/58 (94.83%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGIN KSTV I+A++GD+YVAKIDNKI+VKIGPKMDLGNL+PSN HVATSGQDYAVWE
Sbjct:  364 NGINEKSTVKIMASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNLHVATSGQDYAVWE 421
[Close]
BLAST of FL512401 vs. SwissProt
Match: AMY3C_ORYSJ (Alpha-amylase isozyme 3C OS=Oryza sativa subsp. japonica GN=AMY1.7 PE=2 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 1.033e-15
Identity = 36/58 (62.07%), Postives = 46/58 (79.31%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGI+  S +NILAADGD+YVA ID+K+I KIG + D+GNL+PS+FHV   G +Y VWE
Sbjct:  368 NGIHPGSKLNILAADGDVYVAMIDDKVITKIGTRYDVGNLIPSDFHVVAHGNNYCVWE 425
[Close]
BLAST of FL512401 vs. SwissProt
Match: AMY3E_ORYSJ (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 2.302e-15
Identity = 34/58 (58.62%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGI+ +ST++IL A+GDIYVA ID K+I K+GP+ D G ++PS+FHV   G DY VWE
Sbjct:  367 NGIHAESTLDILKAEGDIYVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWE 424
[Close]
BLAST of FL512401 vs. SwissProt
Match: AMY3A_ORYSJ (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 5.128e-15
Identity = 31/58 (53.45%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGIN  S + +LAA+ D+YVA +D ++I KIGP++D+GN++PS+FH+   G DY VWE
Sbjct:  370 NGINAGSKLRVLAAESDMYVAMVDERVITKIGPRIDVGNIIPSDFHIVAHGNDYCVWE 427
[Close]
BLAST of FL512401 vs. SwissProt
Match: AMY2_HORVU (Alpha-amylase type B isozyme OS=Hordeum vulgare GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 76.2554 bits (186), Expect = 5.669e-14
Identity = 32/58 (55.17%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            +GI+ +S + I+ AD D+Y+A+ID K+IVK+GP+ D+GNL+P  F VA  G DYAVWE
Sbjct:  368 HGIHNESKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFKVAAHGNDYAVWE 425
[Close]
BLAST of FL512401 vs. SwissProt
Match: AMY3B_ORYSJ (Alpha-amylase isozyme 3B OS=Oryza sativa subsp. japonica GN=AMY1.6 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.649e-13
Identity = 32/58 (55.17%), Postives = 44/58 (75.86%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            N I+  S + ILAA+GD+YVA ID+K+I KIG + D+GNL+PS+FHV   G +Y +WE
Sbjct:  368 NEIHPGSKLKILAAEGDVYVAMIDDKVITKIGTRYDVGNLIPSDFHVVAHGNNYCIWE 425
[Close]
BLAST of FL512401 vs. SwissProt
Match: AMY4_HORVU (Alpha-amylase type B isozyme (Fragment) OS=Hordeum vulgare GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.814e-13
Identity = 31/58 (53.45%), Postives = 44/58 (75.86%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            +GI+ +S + I+ AD D+Y+A+ID K+IVK+GP+ D+GNL+P  F  A  G DYAVWE
Sbjct:   94 HGIHNESKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFEGAAHGNDYAVWE 151
[Close]
BLAST of FL512401 vs. SwissProt
Match: AMY5_HORVU (Alpha-amylase type B isozyme (Fragment) OS=Hordeum vulgare GN=AMY1.5 PE=2 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.675e-13
Identity = 31/58 (53.45%), Postives = 44/58 (75.86%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            +GI+ +S + I+ AD D+ +A+ID K+IVK+GP+ D+GNL+P  F VA  G DYAVWE
Sbjct:   76 HGIHSESKLQIIEADADLCLAEIDGKVIVKLGPRYDVGNLIPGGFEVAAHGNDYAVWE 133
[Close]
BLAST of FL512401 vs. SwissProt
Match: AMY3D_ORYSJ (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 73.559 bits (179), Expect = 3.675e-13
Identity = 32/58 (55.17%), Postives = 42/58 (72.41%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGIN  S + I+ AD D YVA +D K++VKIG + D+GN VPS+FH    G+DY+VWE
Sbjct:  367 NGINAGSKLRIVVADADAYVAVVDEKVMVKIGTRYDVGNAVPSDFHQTVHGKDYSVWE 424
[Close]
BLAST of FL512401 vs. SwissProt
Match: AMY6_HORVU (Alpha-amylase type B isozyme OS=Hordeum vulgare GN=AMY1.6 PE=2 SV=2)

HSP 1 Score: 71.2478 bits (173), Expect = 1.824e-12
Identity = 29/57 (50.88%), Postives = 43/57 (75.44%), Query Frame = 3 
Query:    6 GINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            GI+ +S + I+ AD D+Y+A+I+ K+IVK+GP+ D+G+L+P  F V   G DYAVWE
Sbjct:  371 GIHSESKLQIMEADADLYLAEIEGKVIVKLGPRYDVGHLIPEGFKVVAHGNDYAVWE 427
[Close]
BLAST of FL512401 vs. TrEMBL
Match: Q7X9T1_PHAAN (Alpha-amylase OS=Phaseolus angularis PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 7.052e-23
Identity = 51/58 (87.93%), Postives = 56/58 (96.55%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGIN KSTV ILA++GD+YVAKIDNKI+VKIGPKMDLGNL+PSNFHVATSGQDYAVWE
Sbjct:  364 NGINEKSTVKILASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNFHVATSGQDYAVWE 421
[Close]
BLAST of FL512401 vs. TrEMBL
Match: Q9ZP43_PHAVU (Alpha-amylase OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.680e-22
Identity = 50/58 (86.21%), Postives = 55/58 (94.83%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGIN KSTV I+AA+GD+YVAKIDNKI+VKIGPKMDLG L+PSNFHVATSGQDYAVWE
Sbjct:  363 NGINEKSTVEIMAAEGDLYVAKIDNKIMVKIGPKMDLGKLIPSNFHVATSGQDYAVWE 420
[Close]
BLAST of FL512401 vs. TrEMBL
Match: Q42678_CUSRE (Alpha amylase (Fragment) OS=Cuscuta reflexa GN=CUS AMY2 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.682e-16
Identity = 41/58 (70.69%), Postives = 48/58 (82.76%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGI   S+VNILAAD D+YVAK+D+K+IVKIG +MDLGNL+P  F V  SGQDYAVWE
Sbjct:  360 NGITETSSVNILAADHDLYVAKVDDKVIVKIGTRMDLGNLIPPGFKVVASGQDYAVWE 417
[Close]
BLAST of FL512401 vs. TrEMBL
Match: B9NHL8_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_276338 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.682e-16
Identity = 41/57 (71.93%), Postives = 47/57 (82.46%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVW 173
            +GI   STVNILA+D D+YVA ID  +I+KIGPKMDLGNL+PSNF VATSG DY VW
Sbjct:  137 SGIGSASTVNILASDADLYVAAIDGNMIMKIGPKMDLGNLIPSNFQVATSGADYCVW 193
[Close]
BLAST of FL512401 vs. TrEMBL
Match: Q8LP27_IPONI (Alpha-amylase OS=Ipomoea nil GN=PnAmy1 PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 6.393e-16
Identity = 40/58 (68.97%), Postives = 48/58 (82.76%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGI+  S V ILAAD D YVAKID+K+I+KIG K D+GNL+P NF++ TSGQDYAVWE
Sbjct:  364 NGISETSNVEILAADADAYVAKIDDKVIMKIGSKYDVGNLIPPNFNLVTSGQDYAVWE 421
[Close]
BLAST of FL512401 vs. TrEMBL
Match: B9GNW3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_644125 PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 6.393e-16
Identity = 40/57 (70.18%), Postives = 46/57 (80.70%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVW 173
            +GI   STVNILA+D D+YVA ID  +I+KIGPKMDLGNL+PSNF VA SG DY VW
Sbjct:  345 SGIGSASTVNILASDADLYVAAIDGNMIMKIGPKMDLGNLIPSNFQVAASGTDYCVW 401
[Close]
BLAST of FL512401 vs. TrEMBL
Match: B9MVQ5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_918430 PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.424e-15
Identity = 40/56 (71.43%), Postives = 46/56 (82.14%), Query Frame = 3 
Query:    6 GINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVW 173
            GI   STVNILA+D D+YVA I++ II+KIGPK DLGNL+PSNF VATSG DY VW
Sbjct:  366 GIGSASTVNILASDADLYVAAINDNIIMKIGPKTDLGNLIPSNFQVATSGTDYCVW 421
[Close]
BLAST of FL512401 vs. TrEMBL
Match: Q41441_SOLTU (Alpha-amylase (Fragment) OS=Solanum tuberosum GN=Amy23 PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.173e-15
Identity = 39/58 (67.24%), Postives = 47/58 (81.03%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            N I   S V I+A+D D+Y+A I +KIIVKIGPK+DLGNL+P N+ VATSGQDYAVWE
Sbjct:  289 NRICATSNVQIMASDSDLYIAMIHHKIIVKIGPKLDLGNLIPPNYEVATSGQDYAVWE 346
[Close]
BLAST of FL512401 vs. TrEMBL
Match: Q41770_MAIZE (Alpha-amylase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.412e-15
Identity = 37/58 (63.79%), Postives = 47/58 (81.03%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGI+  S +NILAADGD+YVAKID+K+IVKIG + D+GNL+PS+FH    G +Y VWE
Sbjct:  370 NGIHPGSELNILAADGDLYVAKIDDKVIVKIGSRYDVGNLIPSDFHAVAHGNNYCVWE 427
[Close]
BLAST of FL512401 vs. TrEMBL
Match: D7RD61_MAIZE (Alpha amylase OS=Zea mays PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.412e-15
Identity = 37/58 (63.79%), Postives = 47/58 (81.03%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGI+  S +NILAADGD+YVAKID+K+IVKIG + D+GNL+PS+FH    G +Y VWE
Sbjct:  370 NGIHPGSELNILAADGDLYVAKIDDKVIVKIGSRYDVGNLIPSDFHAVAHGNNYCVWE 427
[Close]
BLAST of FL512401 vs. TAIR peptide
Match: AT4G25000.1 (| Symbols: ATAMY1, AMY1 | alpha-amylase-like | chr4:12852109-12853825 REVERSE LENGTH=423)

HSP 1 Score: 78.1814 bits (191), Expect = 1.182e-15
Identity = 36/58 (62.07%), Postives = 46/58 (79.31%), Query Frame = 3 
Query:    3 NGINMKSTVNILAADGDIYVAKIDNKIIVKIGPKMDLGNLVPSNFHVATSGQDYAVWE 176
            NGI   S+V I AA+ D+Y+A ID+K+I+KIGPK D+G LVPSNF +A SG D+AVWE
Sbjct:  364 NGIGSTSSVTIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWE 421
The following BLAST results are available for this feature:
BLAST of FL512401 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
AMYA_VIGMU2.999e-2384.48Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1[more]
AMY3C_ORYSJ1.033e-1562.07Alpha-amylase isozyme 3C OS=Oryza sativa subsp. ja... [more]
AMY3E_ORYSJ2.302e-1558.62Alpha-amylase isozyme 3E OS=Oryza sativa subsp. ja... [more]
AMY3A_ORYSJ5.128e-1553.45Alpha-amylase isozyme 3A OS=Oryza sativa subsp. ja... [more]
AMY2_HORVU5.669e-1455.17Alpha-amylase type B isozyme OS=Hordeum vulgare GN... [more]
AMY3B_ORYSJ1.649e-1355.17Alpha-amylase isozyme 3B OS=Oryza sativa subsp. ja... [more]
AMY4_HORVU2.814e-1353.45Alpha-amylase type B isozyme (Fragment) OS=Hordeum... [more]
AMY5_HORVU3.675e-1353.45Alpha-amylase type B isozyme (Fragment) OS=Hordeum... [more]
AMY3D_ORYSJ3.675e-1355.17Alpha-amylase isozyme 3D OS=Oryza sativa subsp. ja... [more]
AMY6_HORVU1.824e-1250.88Alpha-amylase type B isozyme OS=Hordeum vulgare GN... [more]
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BLAST of FL512401 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q7X9T1_PHAAN7.052e-2387.93Alpha-amylase OS=Phaseolus angularis PE=2 SV=1[more]
Q9ZP43_PHAVU2.680e-2286.21Alpha-amylase OS=Phaseolus vulgaris PE=2 SV=1[more]
Q42678_CUSRE1.682e-1670.69Alpha amylase (Fragment) OS=Cuscuta reflexa GN=CUS... [more]
B9NHL8_POPTR1.682e-1671.93Predicted protein (Fragment) OS=Populus trichocarp... [more]
Q8LP27_IPONI6.393e-1668.97Alpha-amylase OS=Ipomoea nil GN=PnAmy1 PE=2 SV=1[more]
B9GNW3_POPTR6.393e-1670.18Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9MVQ5_POPTR1.424e-1571.43Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q41441_SOLTU3.173e-1567.24Alpha-amylase (Fragment) OS=Solanum tuberosum GN=A... [more]
Q41770_MAIZE5.412e-1563.79Alpha-amylase OS=Zea mays PE=2 SV=1[more]
D7RD61_MAIZE5.412e-1563.79Alpha amylase OS=Zea mays PE=4 SV=1[more]
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BLAST of FL512401 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT4G25000.11.182e-1562.07| Symbols: ATAMY1, AMY1 | alpha-amylase-like | chr... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012850Alpha-amylase, C-terminal beta-sheetPFAMPF07821Alpha-amyl_C2coord: 1..58
score: 1.1
IPR012850Alpha-amylase, C-terminal beta-sheetSMARTSM00810no descriptioncoord: 1..58
score: 3.1
IPR013780Glycosyl hydrolase, family 13, all-betaGENE3D2.60.40.1180no descriptioncoord: 3..58
score: 4.4
NoneNo IPR availablePANTHERPTHR10357AMYLASEcoord: 1..55
score: 4.2
NoneNo IPR availablePANTHERPTHR10357:SF9ALPHA-AMYLASEcoord: 1..55
score: 4.2
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 3..58
score: 5.7

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FL512401 ID=FL512401; Name=FL512401; organism=Cicer arietinum; type=EST; length=326bp
GGAATGGGATCAACATGAAAAGCACAGTGAACATTCTAGCAGCAGATGGT
GACATTTATGTGGCAAAGATAGATAACAAGATTATTGTGAAGATAGGTCC
AAAGATGGACCTTGGAAATCTTGTTCCCTCAAATTTCCACGTTGCTACCT
CTGGTCAAGATTATGCCGTGTGGGAGTGACTATAAATTGAGTGCAATACA
GAATATATTTGGTGTGTTTATCATTAAGTATATATCATGTCTTGTGTGGA
AAAATGCAACTCTCAACTATAATCTTGTATGCTCCATAAAATAAAAACTT
GTTTGTCATACTGTTTTATCCGTTTC
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004556alpha-amylase activity
GO:0005509calcium ion binding
GO:0003824catalytic activity
GO:0043169cation binding
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR012850A-amylase_bs_C
IPR013780Glyco_hydro_b