GR914099

EST Overview


Name	GR914099
Unique Name	GR914099
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	243

Libraries


Library Name	Type
LIBEST_024968 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. JG-62) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR914099 vs. SwissProt
Match: MMT1_WOLBI (Methionine S-methyltransferase OS=Wollastonia biflora GN=MMT1 PE=1 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 7.299e-22
Identity = 44/74 (59.46%), Postives = 59/74 (79.73%), Query Frame = 3

Query:   21 RSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            + L+ VL TV  PWVYISGPTINPTGL+YSN E++++L+ CAR+GAR IIDTS SG++F+   W GW+L+  L+
Sbjct:  825 KQLSSVLETVHKPWVYISGPTINPTGLLYSNEEMKSLLTVCARYGARTIIDTSFSGIKFNSQDWDGWNLDASLA 898

[Close]

BLAST of GR914099 vs. SwissProt
Match: MMT1_MAIZE (Methionine S-methyltransferase OS=Zea mays GN=MMT1 PE=2 SV=2)

HSP 1 Score: 102.064 bits (253), Expect = 9.533e-22
Identity = 43/75 (57.33%), Postives = 56/75 (74.67%), Query Frame = 3

Query:   18 PRSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            P+ L   L  V  PWVY+ GPTINPTG +YS+++I  +LS CA +GARV+IDTS SGLE++ +GW  W+L GCLS
Sbjct:  822 PKVLADTLKNVSRPWVYVCGPTINPTGFLYSDSDIRELLSVCAEYGARVVIDTSFSGLEYETDGWRQWNLAGCLS 896

[Close]

BLAST of GR914099 vs. SwissProt
Match: MMT1_HORVU (Methionine S-methyltransferase OS=Hordeum vulgare GN=MMT1 PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.124e-21
Identity = 46/75 (61.33%), Postives = 55/75 (73.33%), Query Frame = 3

Query:   18 PRSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            P +L   L  V  PWVYISGPTINPTG +YS+++I  +LS CA +GARV+IDTSSSGLEF   G   W+LE CLS
Sbjct:  817 PSALADTLEKVSQPWVYISGPTINPTGFLYSDDDIAELLSVCATYGARVVIDTSSSGLEFQATGCSQWNLERCLS 891

[Close]

BLAST of GR914099 vs. SwissProt
Match: MMT1_ARATH (Methionine S-methyltransferase OS=Arabidopsis thaliana GN=MMT1 PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 3.391e-19
Identity = 45/74 (60.81%), Postives = 57/74 (77.03%), Query Frame = 3

Query:   21 RSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            ++LT  L +VK PWV ISGPT++PTGLVYSN E++ +LSTCA+FGA+VIIDTS SGLE+       WDL+  LS
Sbjct:  813 KTLTKALESVKKPWVCISGPTVSPTGLVYSNEEMDILLSTCAKFGAKVIIDTSFSGLEYSAT---SWDLKNALS 883

[Close]

BLAST of GR914099 vs. TrEMBL
Match: Q1SKS5_MEDTR (SAM (And some other nucleotide) binding motif OS=Medicago truncatula GN=MtrDRAFT_AC140551g25v2 PE=4 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 1.198e-30
Identity = 64/74 (86.49%), Postives = 66/74 (89.19%), Query Frame = 3

Query:   21 RSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            ++LTGVLGTVKNPWVYISGPTINPTGLVYSN EI  IL TCARFGARVIIDTSSSGLEFD  GWGGWDL  CLS
Sbjct:  824 KTLTGVLGTVKNPWVYISGPTINPTGLVYSNKEIGEILITCARFGARVIIDTSSSGLEFDSKGWGGWDLGECLS 897

[Close]

BLAST of GR914099 vs. TrEMBL
Match: B9N0J3_POPTR (Methionine s-methyltransferase OS=Populus trichocarpa GN=MMT PE=4 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.516e-25
Identity = 54/72 (75.00%), Postives = 61/72 (84.72%), Query Frame = 3

Query:   27 LTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            L GVL TV  PWVYISGPTINPTGL+YS+ E+E IL+TC++FGARV+IDTS SGLEFD  GWGGWDLE  LS
Sbjct:  825 LNGVLQTVNKPWVYISGPTINPTGLLYSSKEMETILTTCSKFGARVVIDTSVSGLEFDTEGWGGWDLEPTLS 896

[Close]

BLAST of GR914099 vs. TrEMBL
Match: D7TGK4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_35.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00023133001 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 7.522e-25
Identity = 52/73 (71.23%), Postives = 62/73 (84.93%), Query Frame = 3

Query:   21 RSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCL 239
            ++L GV  +V NPW+YISGPTINPTGLVYSN E+ENILS CA+FGA+V++DTS SGLE+D  G GGWDLEG L
Sbjct:  815 KTLAGVFESVNNPWLYISGPTINPTGLVYSNGEMENILSICAKFGAKVVLDTSFSGLEYDFEGCGGWDLEGIL 887

[Close]

BLAST of GR914099 vs. TrEMBL
Match: A5B4C3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_013862 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 7.522e-25
Identity = 52/73 (71.23%), Postives = 62/73 (84.93%), Query Frame = 3

Query:   21 RSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCL 239
            ++L GV  +V NPW+YISGPTINPTGLVYSN E+ENILS CA+FGA+V++DTS SGLE+D  G GGWDLEG L
Sbjct:  824 KTLAGVFESVNNPWLYISGPTINPTGLVYSNGEMENILSICAKFGAKVVLDTSFSGLEYDFEGCGGWDLEGIL 896

[Close]

BLAST of GR914099 vs. TrEMBL
Match: B9SIP8_RICCO (Methionine S-methyltransferase, putative OS=Ricinus communis GN=RCOM_0825000 PE=4 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 6.368e-24
Identity = 54/68 (79.41%), Postives = 59/68 (86.76%), Query Frame = 3

Query:   27 LTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLE 230
            L GVL TV  PWVYISGPTI PTGL+YSN E+ENIL+TCARFGARVIIDTS SGLEF+  GWGGW+LE
Sbjct:  732 LDGVLDTVNKPWVYISGPTITPTGLLYSNKEMENILTTCARFGARVIIDTSFSGLEFE--GWGGWNLE 797

[Close]

BLAST of GR914099 vs. TrEMBL
Match: C5YXZ8_SORBI (Putative uncharacterized protein Sb09g000490 OS=Sorghum bicolor GN=Sb09g000490 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.201e-22
Identity = 47/75 (62.67%), Postives = 58/75 (77.33%), Query Frame = 3

Query:   18 PRSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            P+ L   L  V  PWVYISGPTINPTG +YS+N+I+ +LS CA +GARV+IDTS SGLE+  +GW  W+LEGCLS
Sbjct:  822 PKVLADTLKNVSQPWVYISGPTINPTGFLYSDNDIQELLSVCAEYGARVVIDTSFSGLEYQTDGWSQWNLEGCLS 896

[Close]

BLAST of GR914099 vs. TrEMBL
Match: B8AWY8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_18110 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.201e-22
Identity = 48/75 (64.00%), Postives = 59/75 (78.67%), Query Frame = 3

Query:   18 PRSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            PR L   L TV  PWVYISGPTINPTG +YS+++I+ +LS CA++GARV+IDTS SGLEF  +GW  W+LE CLS
Sbjct:  812 PRVLADTLETVSRPWVYISGPTINPTGFLYSDSDIQELLSVCAKYGARVVIDTSFSGLEFQTDGWSRWNLERCLS 886

[Close]

BLAST of GR914099 vs. TrEMBL
Match: Q75M18_ORYSJ (Os05g0105000 protein OS=Oryza sativa subsp. japonica GN=P0668H12.19 PE=2 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 3.494e-22
Identity = 47/75 (62.67%), Postives = 58/75 (77.33%), Query Frame = 3

Query:   18 PRSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            PR L   L TV  PWVYISGPTINPTG +YS+++I+ +LS C ++GARV+IDTS SGLEF  +GW  W+LE CLS
Sbjct:  812 PRVLADTLETVSRPWVYISGPTINPTGFLYSDSDIQELLSVCGKYGARVVIDTSFSGLEFQTDGWSRWNLERCLS 886

[Close]

BLAST of GR914099 vs. TrEMBL
Match: D7MPF5_ARALY (Methionine S-methyltransferase OS=Arabidopsis lyrata subsp. lyrata GN=MMT PE=4 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.374e-18
Identity = 46/74 (62.16%), Postives = 57/74 (77.03%), Query Frame = 3

Query:   21 RSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            ++LT  L TVK PWV ISGPT++PTGLVYSN E++ +LSTCA+FGA+VIIDTS SGLE+       WDL+  LS
Sbjct:  812 KTLTNALETVKKPWVCISGPTVSPTGLVYSNEEMDILLSTCAKFGAKVIIDTSFSGLEYSAT---SWDLKSALS 882

[Close]

BLAST of GR914099 vs. TrEMBL
Match: Q0WM74_ARATH (Methionine S-methyltransferase (Fragment) OS=Arabidopsis thaliana GN=At5g49810 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.344e-18
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = 3

Query:   21 RSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            ++LT VL +VK PWV ISGPT++PTGLVYSN E++ +LSTCA+FGA+VIIDTS SGLE+       WDL+  LS
Sbjct:   62 KTLTKVLESVKKPWVCISGPTVSPTGLVYSNEEMDILLSTCAKFGAKVIIDTSFSGLEYSAT---SWDLKNALS 132

[Close]

BLAST of GR914099 vs. TAIR peptide
Match: AT5G49810.1 (| Symbols: MMT | methionine S-methyltransferase | chr5:20239418-20246046 FORWARD LENGTH=1071)

HSP 1 Score: 93.5893 bits (231), Expect = 2.640e-20
Identity = 45/74 (60.81%), Postives = 57/74 (77.03%), Query Frame = 3

Query:   21 RSLTGVLGTVKNPWVYISGPTINPTGLVYSNNEIENILSTCARFGARVIIDTSSSGLEFDCNGWGGWDLEGCLS 242
            ++LT  L +VK PWV ISGPT++PTGLVYSN E++ +LSTCA+FGA+VIIDTS SGLE+       WDL+  LS
Sbjct:  813 KTLTKALESVKKPWVCISGPTVSPTGLVYSNEEMDILLSTCAKFGAKVIIDTSFSGLEYSAT---SWDLKNALS 883

The following BLAST results are available for this feature:

BLAST of GR914099 vs. SwissProt
BLAST of GR914099 vs. TrEMBL
BLAST of GR914099 vs. TAIR peptide

BLAST of GR914099 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
MMT1_WOLBI	7.299e-22	59.46	Methionine S-methyltransferase OS=Wollastonia bifl...	[more]
MMT1_MAIZE	9.533e-22	57.33	Methionine S-methyltransferase OS=Zea mays GN=MMT1...	[more]
MMT1_HORVU	2.124e-21	61.33	Methionine S-methyltransferase OS=Hordeum vulgare ...	[more]
MMT1_ARATH	3.391e-19	60.81	Methionine S-methyltransferase OS=Arabidopsis thal...	[more]

BLAST of GR914099 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q1SKS5_MEDTR	1.198e-30	86.49	SAM (And some other nucleotide) binding motif OS=M...	[more]
B9N0J3_POPTR	1.516e-25	75.00	Methionine s-methyltransferase OS=Populus trichoca...	[more]
D7TGK4_VITVI	7.522e-25	71.23	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5B4C3_VITVI	7.522e-25	71.23	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9SIP8_RICCO	6.368e-24	79.41	Methionine S-methyltransferase, putative OS=Ricinu...	[more]
C5YXZ8_SORBI	1.201e-22	62.67	Putative uncharacterized protein Sb09g000490 OS=So...	[more]
B8AWY8_ORYSI	1.201e-22	64.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q75M18_ORYSJ	3.494e-22	62.67	Os05g0105000 protein OS=Oryza sativa subsp. japoni...	[more]
D7MPF5_ARALY	1.374e-18	62.16	Methionine S-methyltransferase OS=Arabidopsis lyra...	[more]
Q0WM74_ARATH	2.344e-18	62.16	Methionine S-methyltransferase (Fragment) OS=Arabi...	[more]

BLAST of GR914099 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G49810.1	2.640e-20	60.81	\| Symbols: MMT \| methionine S-methyltransferase \| ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015421	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	GENE3D	3.40.640.10	no description	coord: 21..66 score: 0.0

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR914099 ID=GR914099; Name=GR914099; organism=Cicer arietinum; type=EST; length=243bp
GATTAGCGTGGTCGCGGCCGAGGTCACTCACTGGAGTCCTTGGCACCGTG
AAAAACCCATGGGTGTATATTTCTGGACCTACAATCAATCCAACTGGCTT
AGTTTATAGCAACAATGAGATAGAAAACATTTTGAGCACTTGTGCTAGAT
TTGGTGCCAGAGTTATAATTGATACTTCGTCCTCTGGTTTGGAATTTGAC
TGTAACGGCTGGGGTGGCTGGGACTTGGAGGGGTGTTTGTCTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0030170	pyridoxal phosphate binding

Vocabulary: **INTERPRO**
Term	Definition
IPR015421	PyrdxlP-dep_Trfase_major

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