GR916563

EST Overview


Name	GR916563
Unique Name	GR916563
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	446

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR916563 vs. SwissProt
Match: CV013_MOUSE (Uncharacterized protein C22orf13 homolog OS=Mus musculus PE=2 SV=2)

HSP 1 Score: 77.0258 bits (188), Expect = 3.890e-14
Identity = 44/114 (38.60%), Postives = 62/114 (54.39%), Query Frame = 1

Query:    1 DFERPPGQVPHITQIYTWDCGLACILMVLKTIG--DNNCDIQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQ 336
            DF + P  VP I Q+Y WDCGLAC  MVL+ +G  D+     AL EL  T SIWT+DLAYL++             G +  Y  +SFY++    +  RV+ LF +A    + ++
Sbjct:   16 DFVQLP--VPIIQQLYHWDCGLACSRMVLRYLGQLDDGEFENALQELQLTRSIWTIDLAYLMRHFGVRHRFCTQTLGVDKGYKNQSFYRKHFDTEETRVNQLFAQAKACKVQVE 127

[Close]

BLAST of GR916563 vs. SwissProt
Match: CV013_HUMAN (Uncharacterized protein C22orf13 OS=Homo sapiens GN=C22orf13 PE=2 SV=2)

HSP 1 Score: 74.3294 bits (181), Expect = 2.521e-13
Identity = 43/106 (40.57%), Postives = 60/106 (56.60%), Query Frame = 1

Query:    1 DFERPPGQVPHITQIYTWDCGLACILMVLKTIG--DNNCDIQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEA 312
            DF + P  VP I Q+Y WDCGLAC  MVL+ +G  D++   +AL +L  T SIWT+DLAYL+              G +  Y  +SFY++    +  RV+ LF +A
Sbjct:   16 DFVQLP--VPVIQQLYHWDCGLACSRMVLRYLGQLDDSEFERALQKLQLTRSIWTIDLAYLMHHFGVRHRFCTQTLGVDKGYKNQSFYRKHFDTEETRVNQLFAQA 119

[Close]

BLAST of GR916563 vs. TrEMBL
Match: A0FK53_SOYBN (Guanylyl cyclase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 5.955e-53
Identity = 83/105 (79.05%), Postives = 88/105 (83.81%), Query Frame = 1

Query:   22 QVPHITQIYTWDCGLACILMVLKTIGDNNCDIQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQ 336
            +VPHI QIYTWDCGLAC+LMVLKTIG NNCDIQ LAELC TTSIWTVDLAYLLQR            GANPNYC+ESFYKEELPNDL RVD+LFQ+AMEAGIDIQ
Sbjct:   54 EVPHINQIYTWDCGLACVLMVLKTIGVNNCDIQTLAELCCTTSIWTVDLAYLLQRFAVAFSYFTVTFGANPNYCIESFYKEELPNDLVRVDLLFQKAMEAGIDIQ 158

HSP 2 Score: 56.225 bits (134), Expect = 5.955e-53
Identity = 28/42 (66.67%), Postives = 34/42 (80.95%), Query Frame = 2

Query:  320 LELIYRSISEQEISFLILSGKYIGIALVDHSKLSHAGQDVHV 445
            +++  RSIS +EIS LILSGKY+ IALVDH+KLSH  QDV V
Sbjct:  155 IDIQCRSISAEEISILILSGKYVAIALVDHNKLSHVLQDVPV 196

[Close]

BLAST of GR916563 vs. TrEMBL
Match: B9SE40_RICCO (Protein C22orf13, putative OS=Ricinus communis GN=RCOM_1483470 PE=4 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 1.124e-41
Identity = 70/105 (66.67%), Postives = 82/105 (78.10%), Query Frame = 1

Query:   22 QVPHITQIYTWDCGLACILMVLKTIGDNNCDIQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQ 336
            +VPH++Q+++WDCGLAC+LM L TIG NNC IQALAELCSTTSIWTVDLAYLLQ+            GANPNY  E+FYKE+LP DL RVD LFQ+A E GI+IQ
Sbjct:   23 EVPHVSQLHSWDCGLACVLMALNTIGINNCSIQALAELCSTTSIWTVDLAYLLQKFSVRFSYFTVTLGANPNYSAETFYKEQLPTDLVRVDRLFQKAREKGINIQ 127

HSP 2 Score: 43.1282 bits (100), Expect = 1.124e-41
Identity = 21/31 (67.74%), Postives = 25/31 (80.65%), Query Frame = 2

Query:  335 RSISEQEISFLILSGKYIGIALVDHSKLSHA 427
            RSI+E+EIS  ILSGKYI +ALVD  KLS +
Sbjct:  129 RSINEKEISLFILSGKYIAVALVDQYKLSRS 159

[Close]

BLAST of GR916563 vs. TrEMBL
Match: B9HHJ1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_720589 PE=4 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 2.070e-40
Identity = 69/105 (65.71%), Postives = 80/105 (76.19%), Query Frame = 1

Query:   22 QVPHITQIYTWDCGLACILMVLKTIGDNNCDIQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQ 336
            QVPHI Q+++WDCGLAC+LM L TIG NNC IQ LA+LC T+SIWTVDLAYLLQ+            GANPNY VE+FYKE+LP DL RVDMLFQ+A   GI+IQ
Sbjct:   53 QVPHIKQLHSWDCGLACVLMALNTIGINNCSIQGLADLCCTSSIWTVDLAYLLQKYSVSFSFYTVTLGANPNYSVETFYKEQLPADLVRVDMLFQKARGEGINIQ 157

HSP 2 Score: 43.8986 bits (102), Expect = 2.070e-40
Identity = 22/31 (70.97%), Postives = 25/31 (80.65%), Query Frame = 2

Query:  335 RSISEQEISFLILSGKYIGIALVDHSKLSHA 427
            RSI+E EIS  ILSGKYI IALV+  KLSH+
Sbjct:  159 RSINETEISLFILSGKYIAIALVNQYKLSHS 189

[Close]

BLAST of GR916563 vs. TrEMBL
Match: Q0PY32_IPONI (Guanylyl cyclase OS=Ipomoea nil PE=2 SV=2)

HSP 1 Score: 142.124 bits (357), Expect = 1.427e-38
Identity = 63/105 (60.00%), Postives = 84/105 (80.00%), Query Frame = 1

Query:   22 QVPHITQIYTWDCGLACILMVLKTIGDNNCDIQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQ 336
            +VPH+ Q+YTWDCGLAC++MVL+T+G  N +I+ LA+ CSTTSIWTVDLAY+L++            GANPN+CVE+FYKE+LP+DL RV+ LFQ+A EAGI+I+
Sbjct:   64 EVPHVKQLYTWDCGLACVVMVLRTLGIYNSNIEELAQSCSTTSIWTVDLAYMLRKFSVNFSYFTITIGANPNFCVETFYKEQLPSDLARVNTLFQKAREAGINIE 168

HSP 2 Score: 41.5874 bits (96), Expect = 1.427e-38
Identity = 21/30 (70.00%), Postives = 23/30 (76.67%), Query Frame = 2

Query:  335 RSISEQEISFLILSGKYIGIALVDHSKLSH 424
            RSIS +E+  LILSG  I IALVDH KLSH
Sbjct:  170 RSISGEEMCMLILSGNCIAIALVDHHKLSH 199

[Close]

BLAST of GR916563 vs. TrEMBL
Match: A0FK54_SOLLC (Guanylyl cyclase OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.669e-36
Identity = 61/104 (58.65%), Postives = 79/104 (75.96%), Query Frame = 1

Query:   25 VPHITQIYTWDCGLACILMVLKTIGDNNCDIQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQ 336
            VPHI Q+++WDCGLAC+LMVL+ +G  + ++Q L E C TTSIWTVDLAYLL++            GANP++ VE+FYKE+LPNDL RVDMLFQ+A +AGI I+
Sbjct:   52 VPHINQLHSWDCGLACVLMVLRFLGIKDGNMQELEEFCCTTSIWTVDLAYLLKKFSVSFSYFTVTLGANPSFSVETFYKEQLPNDLVRVDMLFQKARDAGISIE 155

HSP 2 Score: 44.2838 bits (103), Expect = 1.669e-36
Identity = 22/35 (62.86%), Postives = 26/35 (74.29%), Query Frame = 2

Query:  335 RSISEQEISFLILSGKYIGIALVDHSKLSHAGQDV 439
            RSIS +EIS LILSG ++ I LVD  KLSH+  DV
Sbjct:  157 RSISSEEISLLILSGNFLAITLVDQYKLSHSWLDV 191

[Close]

BLAST of GR916563 vs. TrEMBL
Match: D7TML6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_19.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016433001 PE=4 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.123e-34
Identity = 71/118 (60.17%), Postives = 88/118 (74.58%), Query Frame = 1

Query:   22 QVPHITQIYTWDCGLACILMVLKTIGDNNCDIQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQVN**ARDFLSHIV 375
            +VPH+ Q+ TWDCGLAC+LMVL+T G NNC+IQAL ELC TTSIWTVDLAYLLQ+            GANPN+ VE+FYK++L  DL RVD LF++AMEAGIDIQ    + D +S ++
Sbjct:   56 EVPHMNQLSTWDCGLACVLMVLRTFGINNCNIQALEELCCTTSIWTVDLAYLLQKFSVSFSYFTVTLGANPNFSVETFYKDQLATDLVRVDSLFKKAMEAGIDIQCRSISGDEISLLI 173

[Close]

BLAST of GR916563 vs. TrEMBL
Match: A5C221_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_014350 PE=4 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.123e-34
Identity = 71/118 (60.17%), Postives = 88/118 (74.58%), Query Frame = 1

Query:   22 QVPHITQIYTWDCGLACILMVLKTIGDNNCDIQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQVN**ARDFLSHIV 375
            +VPH+ Q+ TWDCGLAC+LMVL+T G NNC+IQAL ELC TTSIWTVDLAYLLQ+            GANPN+ VE+FYK++L  DL RVD LF++AMEAGIDIQ    + D +S ++
Sbjct:   56 EVPHMNQLSTWDCGLACVLMVLRTFGINNCNIQALEELCCTTSIWTVDLAYLLQKFSVSFSYFTVTLGANPNFSVETFYKDQLATDLVRVDSLFKKAMEAGIDIQCRSISGDEISLLI 173

[Close]

BLAST of GR916563 vs. TrEMBL
Match: Q2EN86_ORYSA (Guanylyl cyclase OS=Oryza sativa PE=2 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.952e-32
Identity = 61/105 (58.10%), Postives = 74/105 (70.48%), Query Frame = 1

Query:   25 VPHITQIYTWDCGLACILMVLKTIGDNNCD-IQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQ 336
            VPH+ Q +TWDCGLAC+LMVL+T+G + CD I  L +LC TTSIWTVDLAYLL R            GANP Y  E+FY+E+L  D++RVD LF  A+EAGI IQ
Sbjct:   46 VPHVQQAFTWDCGLACVLMVLRTLGMDCCDGIADLEKLCRTTSIWTVDLAYLLNRFSVCFSFFTVTLGANPQYSAETFYREQLQEDIDRVDELFGRALEAGISIQ 150

HSP 2 Score: 35.8094 bits (81), Expect = 2.952e-32
Identity = 18/29 (62.07%), Postives = 23/29 (79.31%), Query Frame = 2

Query:  335 RSISEQEISFLILSGKYIGIALVDHSKLS 421
            RSIS  +I+FL+LSG  I IALVD +KL+
Sbjct:  152 RSISAYDIAFLLLSGHCIAIALVDKTKLN 180

[Close]

BLAST of GR916563 vs. TrEMBL
Match: B8AYT0_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_20176 PE=4 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.952e-32
Identity = 61/105 (58.10%), Postives = 74/105 (70.48%), Query Frame = 1

Query:   25 VPHITQIYTWDCGLACILMVLKTIGDNNCD-IQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQ 336
            VPH+ Q +TWDCGLAC+LMVL+T+G + CD I  L +LC TTSIWTVDLAYLL R            GANP Y  E+FY+E+L  D++RVD LF  A+EAGI IQ
Sbjct:   46 VPHVQQAFTWDCGLACVLMVLRTLGMDCCDGIADLEKLCRTTSIWTVDLAYLLNRFSVCFSFFTVTLGANPQYSAETFYREQLQEDIDRVDELFGRALEAGISIQ 150

HSP 2 Score: 35.8094 bits (81), Expect = 2.952e-32
Identity = 18/29 (62.07%), Postives = 23/29 (79.31%), Query Frame = 2

Query:  335 RSISEQEISFLILSGKYIGIALVDHSKLS 421
            RSIS  +I+FL+LSG  I IALVD +KL+
Sbjct:  152 RSISAYDIAFLLLSGHCIAIALVDKTKLN 180

[Close]

BLAST of GR916563 vs. TrEMBL
Match: Q2EN88_MAIZE (Guanylyl cyclase-like protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 6.522e-32
Identity = 60/105 (57.14%), Postives = 74/105 (70.48%), Query Frame = 1

Query:   25 VPHITQIYTWDCGLACILMVLKTIGDNNCD-IQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQ 336
            VPH+ Q +TWDCGLAC+LMVL+T+G + CD I  L  LC TTSIWTVDLAYLL +            GANP Y  ESFY+E+L  D++RVD LF +A++AGI IQ
Sbjct:   44 VPHVQQAFTWDCGLACVLMVLRTLGIDCCDGIADLERLCRTTSIWTVDLAYLLNKFSVSFSFCTVTLGANPQYSAESFYREQLQEDIDRVDELFGKALDAGISIQ 148

HSP 2 Score: 35.8094 bits (81), Expect = 6.522e-32
Identity = 18/29 (62.07%), Postives = 23/29 (79.31%), Query Frame = 2

Query:  335 RSISEQEISFLILSGKYIGIALVDHSKLS 421
            RSI+  +I+FL+LSG  I IALVD SKL+
Sbjct:  150 RSITAYDIAFLLLSGHCIAIALVDKSKLN 178

[Close]

BLAST of GR916563 vs. TAIR peptide
Match: AT5G05930.1 (| Symbols: ATGC1, GC1 | guanylyl cyclase 1 | chr5:1780270-1782659 REVERSE LENGTH=274)

HSP 1 Score: 132.494 bits (332), Expect = 9.299e-32
Identity = 61/104 (58.65%), Postives = 77/104 (74.04%), Query Frame = 1

Query:   25 VPHITQIYTWDCGLACILMVLKTIGDNNCDIQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQ 336
            VPH+ Q+ +WDCGLAC+LMVL+  G  +C ++ LAE+CST SIWTVDLAYLLQ+            GANPNY +E FYKE+LP DL RVD+LF++A E+GI IQ
Sbjct:   59 VPHVHQLASWDCGLACVLMVLRASGIASCTLEDLAEICSTNSIWTVDLAYLLQKFCVEFSYYTITFGANPNYSIEEFYKEQLPEDLVRVDLLFRKAHESGIIIQ 162

[Close]

BLAST of GR916563 vs. TAIR peptide
Match: AT5G05930.2 (| Symbols: GC1 | guanylyl cyclase 1 | chr5:1780270-1782532 REVERSE LENGTH=210)

HSP 1 Score: 95.9005 bits (237), Expect = 9.646e-21
Identity = 46/80 (57.50%), Postives = 57/80 (71.25%), Query Frame = 1

Query:   97 GDNNCDIQALAELCSTTSIWTVDLAYLLQRXXXXXXXXXXXXGANPNYCVESFYKEELPNDLERVDMLFQEAMEAGIDIQ 336
            G  +C ++ LAE+CST SIWTVDLAYLLQ+            GANPNY +E FYKE+LP DL RVD+LF++A E+GI IQ
Sbjct:   19 GIASCTLEDLAEICSTNSIWTVDLAYLLQKFCVEFSYYTITFGANPNYSIEEFYKEQLPEDLVRVDLLFRKAHESGIIIQ 98

The following BLAST results are available for this feature:

BLAST of GR916563 vs. SwissProt
BLAST of GR916563 vs. TrEMBL
BLAST of GR916563 vs. TAIR peptide

BLAST of GR916563 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
CV013_MOUSE	3.890e-14	38.60	Uncharacterized protein C22orf13 homolog OS=Mus mu...	[more]
CV013_HUMAN	2.521e-13	40.57	Uncharacterized protein C22orf13 OS=Homo sapiens G...	[more]

BLAST of GR916563 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A0FK53_SOYBN	5.955e-53	79.05	Guanylyl cyclase OS=Glycine max PE=2 SV=1	[more]
B9SE40_RICCO	1.124e-41	66.67	Protein C22orf13, putative OS=Ricinus communis GN=...	[more]
B9HHJ1_POPTR	2.070e-40	65.71	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q0PY32_IPONI	1.427e-38	60.00	Guanylyl cyclase OS=Ipomoea nil PE=2 SV=2	[more]
A0FK54_SOLLC	1.669e-36	58.65	Guanylyl cyclase OS=Solanum lycopersicum PE=2 SV=1	[more]
D7TML6_VITVI	3.123e-34	60.17	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5C221_VITVI	3.123e-34	60.17	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q2EN86_ORYSA	2.952e-32	58.10	Guanylyl cyclase OS=Oryza sativa PE=2 SV=1	[more]
B8AYT0_ORYSI	2.952e-32	58.10	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q2EN88_MAIZE	6.522e-32	57.14	Guanylyl cyclase-like protein OS=Zea mays PE=2 SV=...	[more]

BLAST of GR916563 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G05930.1	9.299e-32	58.65	\| Symbols: ATGC1, GC1 \| guanylyl cyclase 1 \| chr5:...	[more]
AT5G05930.2	9.646e-21	57.50	\| Symbols: GC1 \| guanylyl cyclase 1 \| chr5:1780270...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005074	Peptidase C39, bacteriocin processing	PFAM	PF03412	Peptidase_C39	coord: 13..49 score: 0.0
IPR018616	Guanylyl cyclase	PFAM	PF09778	Guanylate_cyc_2	coord: 9..113 score: 2.8
None	No IPR available	SEG	seg	seg	coord: 63..74 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR916563 ID=GR916563; Name=GR916563; organism=Cicer arietinum; type=EST; length=446bp
GATTTCGAGCGGCCGCCCGGGCAGGTACCTCATATAACTCAAATATATAC
ATGGGATTGTGGCCTTGCTTGTATATTAATGGTTCTGAAAACTATTGGTG
ACAACAATTGCGATATTCAAGCACTGGCTGAACTATGCTCCACAACTAGC
ATTTGGACTGTTGATTTGGCATATTTGTTACAGAGATTTTCTGTTACTTT
TTCCTACTTCACAGTGACATTTGGTGCGAACCCAAACTATTGTGTTGAAT
CCTTTTACAAGGAGGAATTGCCTAATGATCTAGAAAGGGTGGATATGCTG
TTTCAGGAAGCAATGGAGGCTGGAATTGATATACAGGTCAATTAGTGAGC
AAGAGATTTCCTTTCTCATATTGTCTGGGAAATATATAGGCATTGCATTA
GTTGATCACAGTAAATTGAGTCATGCAGGGCAAGATGTTCATGTCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005524	ATP binding
GO:0008233	peptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR005074	Peptidase C39, bacteriocin processing
IPR018616	GUCD1

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