GR917858

EST Overview
NameGR917858
Unique NameGR917858
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length244
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
BLAST of GR917858 vs. SwissProt
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 5.426e-9
Identity = 25/34 (73.53%), Postives = 31/34 (91.18%), Query Frame = -3
Query:   42 DMIDMAERDGFVLTKLIPLKAMCKTRPVVNRLIK 143
            D ID+AERDGF LTKL+PLKAMCK+RPV+N++ K
Sbjct:  465 DKIDLAERDGFTLTKLVPLKAMCKSRPVINKVFK 498          
BLAST of GR917858 vs. TrEMBL
Match: Q8L6V5_CICAR (Putative cytochrome P450 monooxygenase (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.471e-12
Identity = 36/37 (97.30%), Postives = 37/37 (100.00%), Query Frame = -3
Query:   42 TSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRLIK 152
            TSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNR+IK
Sbjct:   27 TSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 63          
BLAST of GR917858 vs. TrEMBL
Match: Q9XFX0_CICAR (Cytochrome P450 monooxygenase OS=Cicer arietinum GN=cyp81E3v2 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.246e-11
Identity = 33/37 (89.19%), Postives = 36/37 (97.30%), Query Frame = -3
Query:   42 TSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRLIK 152
            TSDDMIDMAERDGFVLTKL+PLKAMCKTRPVVN++ K
Sbjct:  462 TSDDMIDMAERDGFVLTKLVPLKAMCKTRPVVNKIFK 498          
BLAST of GR917858 vs. TrEMBL
Match: Q9ZRW6_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.182e-11
Identity = 33/37 (89.19%), Postives = 35/37 (94.59%), Query Frame = -3
Query:   42 TSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRLIK 152
            TSDD IDMAERDGFVLTKLIPLKAMCKTRPVVN++ K
Sbjct:  462 TSDDKIDMAERDGFVLTKLIPLKAMCKTRPVVNKVFK 498          
BLAST of GR917858 vs. TrEMBL
Match: Q9ZWF2_GLYEC (Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-31 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.289e-8
Identity = 27/37 (72.97%), Postives = 33/37 (89.19%), Query Frame = -3
Query:   42 TSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRLIK 152
            T+ D IDMAERDGF LTKL+PLKAMCK+RPV+N++ K
Sbjct:  462 TNGDKIDMAERDGFTLTKLVPLKAMCKSRPVINKVFK 498          
BLAST of GR917858 vs. TrEMBL
Match: Q6WNR0_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.872e-8
Identity = 26/36 (72.22%), Postives = 33/36 (91.67%), Query Frame = -3
Query:   42 SDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRLIK 149
            +D+ IDM+ERDGF +TKL+PLKAMCKTRPVVN++ K
Sbjct:  463 NDEKIDMSERDGFTMTKLLPLKAMCKTRPVVNKVFK 498          
BLAST of GR917858 vs. TrEMBL
Match: Q9MBE4_LOTJA (Cytochrome P450 OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.238e-7
Identity = 25/36 (69.44%), Postives = 30/36 (83.33%), Query Frame = -3
Query:   42 SDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRLIK 149
            SD+ IDM ERDGFVL K IP+KAMCK+RPV+N + K
Sbjct:  464 SDEEIDMGERDGFVLMKSIPVKAMCKSRPVINNVFK 499          
The following BLAST results are available for this feature:
BLAST of GR917858 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1
Match NameE-valueIdentityDescription
C81E1_GLYEC5.426e-973.53Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata ... [more]
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BLAST of GR917858 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 6
Match NameE-valueIdentityDescription
Q8L6V5_CICAR1.471e-1297.30Putative cytochrome P450 monooxygenase (Fragment) ... [more]
Q9XFX0_CICAR1.246e-1189.19Cytochrome P450 monooxygenase OS=Cicer arietinum G... [more]
Q9ZRW6_CICAR6.182e-1189.19Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2... [more]
Q9ZWF2_GLYEC1.289e-872.97Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-... [more]
Q6WNR0_MEDTR2.872e-872.22Isoflavone 2'-hydroxylase OS=Medicago truncatula P... [more]
Q9MBE4_LOTJA9.238e-769.44Cytochrome P450 OS=Lotus japonicus PE=2 SV=1[more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007770Protein of unknown function DUF679PFAMPF05078DUF679coord: 4..44
score: 8.4

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GR917858 ID=GR917858; Name=GR917858; organism=Cicer arietinum; type=EST; length=244bp
GATTAGCGTGGTCGCGGCCGAGGTACCGTGAGATTTACTTACTTGATAAG
TCTGTTTACGACCGGACGAGTTTTACACATGGCCTTTAATGGAATTAATT
TTGTCAAAACAAATCCATCTCGTTCTGCCATATCAATCATGTCATCACTT
GTACCTGTGTAGCTATCTGTGAAAGTGGAAAAGATGCAATTGAAACCGCA
TATTGCAAGAAGAATTGAACTTAGGTACCTGCCCGGGCGGTCGC
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: INTERPRO
TermDefinition
IPR007770DMP