CK148994

EST Overview
NameCK148994
Unique NameCK148994
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length481
Libraries
Library NameType
LIBEST_014674 Suppressive subtractive hybridization librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of CK148994 vs. TrEMBL
Match: Q9C6L9_ARATH (Putative uncharacterized protein F2J7.7 OS=Arabidopsis thaliana GN=At1g25500 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 7.282e-14
Identity = 33/55 (60.00%), Postives = 39/55 (70.91%), Query Frame = -3 
Query:  315 SDRVIMIGSTSXXXXXXXXXXXXXXVESAVTSIYICYAEDPLLIQRWDAEFFNQM 479
            SDRVIM+ ST+              VESAVTSIYIC+AEDPLLIQRWDA+F+ +M
Sbjct:  412 SDRVIMVASTAMLMGMVLVGLGMVVVESAVTSIYICFAEDPLLIQRWDADFYTEM 466

HSP 2 Score: 33.4982 bits (75), Expect = 7.282e-14
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = -2 
Query:  256 ETLHHRLQYRSARAREVLT 312
            E LH RLQ+RS+RAREVLT
Sbjct:  468 EVLHRRLQHRSSRAREVLT 486
[Close]
BLAST of CK148994 vs. TrEMBL
Match: Q56X79_ARATH (Putative uncharacterized protein At1g25500 OS=Arabidopsis thaliana GN=At1g25500 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 7.418e-14
Identity = 33/55 (60.00%), Postives = 39/55 (70.91%), Query Frame = -3 
Query:  315 SDRVIMIGSTSXXXXXXXXXXXXXXVESAVTSIYICYAEDPLLIQRWDAEFFNQM 479
            SDRVIM+ ST+              VESAVTSIYIC+AEDPLLIQRWDA+F+ +M
Sbjct:  184 SDRVIMVASTAMLMGMVLVGLGMVVVESAVTSIYICFAEDPLLIQRWDADFYTEM 238

HSP 2 Score: 33.4982 bits (75), Expect = 7.418e-14
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = -2 
Query:  256 ETLHHRLQYRSARAREVLT 312
            E LH RLQ+RS+RAREVLT
Sbjct:  240 EVLHRRLQHRSSRAREVLT 258
[Close]
BLAST of CK148994 vs. TrEMBL
Match: Q6EP45_ORYSJ (Choline transporter-like protein OS=Oryza sativa subsp. japonica GN=B1267B06.27 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.227e-13
Identity = 34/55 (61.82%), Postives = 38/55 (69.09%), Query Frame = -3 
Query:  315 SDRVIMIGSTSXXXXXXXXXXXXXXVESAVTSIYICYAEDPLLIQRWDAEFFNQM 479
            SD+ IM+GSTS              VESAVTSIYICYAEDP LIQRWD +FF+QM
Sbjct:  437 SDKAIMVGSTSMLMGMILVGVTVVVVESAVTSIYICYAEDPRLIQRWDPDFFDQM 491

HSP 2 Score: 31.9574 bits (71), Expect = 1.227e-13
Identity = 16/24 (66.67%), Postives = 19/24 (79.17%), Query Frame = -2 
Query:  241 ETLHHRLQYRSARAREVLTHYRLD 312
            E LH RLQYRSARA ++L + RLD
Sbjct:  493 EALHQRLQYRSARAHQIL-NSRLD 515
[Close]
BLAST of CK148994 vs. TrEMBL
Match: D7KAH9_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_313379 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 3.502e-13
Identity = 33/55 (60.00%), Postives = 39/55 (70.91%), Query Frame = -3 
Query:  315 SDRVIMIGSTSXXXXXXXXXXXXXXVESAVTSIYICYAEDPLLIQRWDAEFFNQM 479
            SDRVIM+ ST+              VESAVTSIYIC+AEDPLLIQRWDA+F+ +M
Sbjct:  412 SDRVIMVASTAMLMGMVLVGLGMVVVESAVTSIYICFAEDPLLIQRWDADFYTEM 466

HSP 2 Score: 31.187 bits (69), Expect = 3.502e-13
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = -2 
Query:  256 ETLHHRLQYRSARAREVLT 312
            E LH RLQ+RS+RAREV T
Sbjct:  468 EVLHRRLQHRSSRAREVST 486
[Close]
BLAST of CK148994 vs. TrEMBL
Match: D7T979_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011930001 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.286e-12
Identity = 38/55 (69.09%), Postives = 40/55 (72.73%), Query Frame = -3 
Query:  315 SDRVIMIGSTSXXXXXXXXXXXXXXVESAVTSIYICYAEDPLLIQRWDAEFFNQM 479
            SDRVIM+GST+              VESAVTSIYICYAEDPLLI RWDAEFFNQM
Sbjct:  416 SDRVIMVGSTAMLMGMILVGLAMVVVESAVTSIYICYAEDPLLIHRWDAEFFNQM 470
[Close]
BLAST of CK148994 vs. TrEMBL
Match: B9ST27_RICCO (Protein PNS1, putative OS=Ricinus communis GN=RCOM_0353730 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.631e-11
Identity = 36/55 (65.45%), Postives = 40/55 (72.73%), Query Frame = -3 
Query:  315 SDRVIMIGSTSXXXXXXXXXXXXXXVESAVTSIYICYAEDPLLIQRWDAEFFNQM 479
            +DRVIM+GST+              VESAVTSIYICYAEDPLLI RWDAEFFNQ+
Sbjct:  409 NDRVIMVGSTAMLMGMVLVGVAMVVVESAVTSIYICYAEDPLLIHRWDAEFFNQL 463
[Close]
BLAST of CK148994 vs. TrEMBL
Match: C5XVR7_SORBI (Putative uncharacterized protein Sb04g024030 OS=Sorghum bicolor GN=Sb04g024030 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.197e-11
Identity = 35/55 (63.64%), Postives = 38/55 (69.09%), Query Frame = -3 
Query:  315 SDRVIMIGSTSXXXXXXXXXXXXXXVESAVTSIYICYAEDPLLIQRWDAEFFNQM 479
            SD+ +M+GSTS              VESAVTSIYICYAEDPLLIQRWD EFF QM
Sbjct:  444 SDKAVMVGSTSMLMGMILVGLTVVVVESAVTSIYICYAEDPLLIQRWDPEFFEQM 498
[Close]
BLAST of CK148994 vs. TrEMBL
Match: B6TKE2_MAIZE (Putative uncharacterized protein OS=Zea mays PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.197e-11
Identity = 35/55 (63.64%), Postives = 38/55 (69.09%), Query Frame = -3 
Query:  315 SDRVIMIGSTSXXXXXXXXXXXXXXVESAVTSIYICYAEDPLLIQRWDAEFFNQM 479
            SD+ +M+GSTS              VESAVTSIYICYAEDPLLIQRWD EFF QM
Sbjct:   23 SDKAVMVGSTSMLMGMILVGLTVVVVESAVTSIYICYAEDPLLIQRWDPEFFEQM 77
[Close]
BLAST of CK148994 vs. TAIR peptide
Match: AT1G25500.2 (| Symbols: | Plasma-membrane choline transporter family protein | chr1:8955437-8957793 REVERSE LENGTH=488)

HSP 1 Score: 67.0106 bits (162), Expect = 5.904e-16
Identity = 33/55 (60.00%), Postives = 39/55 (70.91%), Query Frame = -3 
Query:  315 SDRVIMIGSTSXXXXXXXXXXXXXXVESAVTSIYICYAEDPLLIQRWDAEFFNQM 479
            SDRVIM+ ST+              VESAVTSIYIC+AEDPLLIQRWDA+F+ +M
Sbjct:  412 SDRVIMVASTAMLMGMVLVGLGMVVVESAVTSIYICFAEDPLLIQRWDADFYTEM 466

HSP 2 Score: 33.4982 bits (75), Expect = 5.904e-16
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = -2 
Query:  256 ETLHHRLQYRSARAREVLT 312
            E LH RLQ+RS+RAREVLT
Sbjct:  468 EVLHRRLQHRSSRAREVLT 486
[Close]
BLAST of CK148994 vs. TAIR peptide
Match: AT1G25500.3 (| Symbols: | Plasma-membrane choline transporter family protein | chr1:8955437-8957294 REVERSE LENGTH=430)

HSP 1 Score: 67.0106 bits (162), Expect = 5.923e-16
Identity = 33/55 (60.00%), Postives = 39/55 (70.91%), Query Frame = -3 
Query:  315 SDRVIMIGSTSXXXXXXXXXXXXXXVESAVTSIYICYAEDPLLIQRWDAEFFNQM 479
            SDRVIM+ ST+              VESAVTSIYIC+AEDPLLIQRWDA+F+ +M
Sbjct:  354 SDRVIMVASTAMLMGMVLVGLGMVVVESAVTSIYICFAEDPLLIQRWDADFYTEM 408

HSP 2 Score: 33.4982 bits (75), Expect = 5.923e-16
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = -2 
Query:  256 ETLHHRLQYRSARAREVLT 312
            E LH RLQ+RS+RAREVLT
Sbjct:  410 EVLHRRLQHRSSRAREVLT 428
The following BLAST results are available for this feature:
BLAST of CK148994 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 8
Match NameE-valueIdentityDescription
Q9C6L9_ARATH7.282e-1460.00Putative uncharacterized protein F2J7.7 OS=Arabido... [more]
Q56X79_ARATH7.418e-1460.00Putative uncharacterized protein At1g25500 OS=Arab... [more]
Q6EP45_ORYSJ1.227e-1361.82Choline transporter-like protein OS=Oryza sativa s... [more]
D7KAH9_ARALY3.502e-1360.00Putative uncharacterized protein OS=Arabidopsis ly... [more]
D7T979_VITVI3.286e-1269.09Whole genome shotgun sequence of line PN40024, sca... [more]
B9ST27_RICCO1.631e-1165.45Protein PNS1, putative OS=Ricinus communis GN=RCOM... [more]
C5XVR7_SORBI6.197e-1163.64Putative uncharacterized protein Sb04g024030 OS=So... [more]
B6TKE2_MAIZE6.197e-1163.64Putative uncharacterized protein OS=Zea mays PE=4 ... [more]
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BLAST of CK148994 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT1G25500.25.904e-1660.00| Symbols: | Plasma-membrane choline transporter ... [more]
AT1G25500.35.923e-1660.00| Symbols: | Plasma-membrane choline transporter ... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007603Choline transporter-likePANTHERPTHR12385CTL TRANSPORTERcoord: 10..69
score: 1.3
IPR007603Choline transporter-likePFAMPF04515Choline_transpocoord: 4..46
score: 1.3
NoneNo IPR availablePANTHERPTHR12385:SF4CTL2-RELATEDcoord: 10..69
score: 1.3
NoneNo IPR availableSEGsegsegcoord: 12..26
score

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>CK148994 ID=CK148994; Name=CK148994; organism=Cicer arietinum; type=EST; length=481bp
TAACGGGGTCGCGTACGAGTGTCACATGGCGACGTTATAACTCATGCTAC
TACACTACAATGTACTTTTCAGTTCACTCACATCAATCTAACATTGTCGA
TTACACTACCAGAAACTTTATCCTATTCCTAATAATTTAACACACCATGT
TAATATGCTACATAAATAAACTATTGTAGAACAAAATAAAACAATGCATG
TGAATCTTTAAATAGATGCGTTTTCTCGTATACGATCATCATCGAGTCGA
TAGTGGGTTAAAACTTCCCTAGCTCGAGCGCTTCTATATTGAAGTCGATG
ATGTAGTGTCTCAACATCTGGTTGAAAAATTCTGCATCCCACCTTTGAAT
CAATAAAGGGTCTTCTGCATAACAAATATATATCGATGTAACAGCACTCT
CCACCACAACCATTGCCAATCCAACCAAAACCATACCCATTAGCATGGAT
GTGGACCCAATCATAATAACTCTGTCACTCC
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: INTERPRO
TermDefinition
IPR007603Choline_transptr-like