FE670547

EST Overview


Name	FE670547
Unique Name	FE670547
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	251

Libraries


Library Name	Type
LIBEST_022821 Chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FE670547 vs. SwissProt
Match: CYT10_ORYSJ (Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. japonica GN=Os04g0350100 PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 8.287e-10
Identity = 37/84 (44.05%), Postives = 51/84 (60.71%), Query Frame = 1

Query:   10 STASCGRVLVGAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGGEA------LKFVEVVEAHQQVVSGMKYYLNISTTDR 243
            + A    VLVG +TEI D  +N+ V  LG+FAV E+N ++  + G GG A      L F  VVEA +QVVSG+ YYL ++ + R
Sbjct:   30 AAAPAAPVLVGGRTEIRDVGSNKAVQSLGRFAVAEHN-RRLRHGGSGGPADPVPVKLAFARVVEAQKQVVSGVAYYLKVAASAR 112

[Close]

BLAST of FE670547 vs. SwissProt
Match: CYT10_ORYSI (Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. indica GN=OSIGBa0144C23.1 PE=2 SV=2)

HSP 1 Score: 62.3882 bits (150), Expect = 8.287e-10
Identity = 37/84 (44.05%), Postives = 51/84 (60.71%), Query Frame = 1

Query:   10 STASCGRVLVGAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGGEA------LKFVEVVEAHQQVVSGMKYYLNISTTDR 243
            + A    VLVG +TEI D  +N+ V  LG+FAV E+N ++  + G GG A      L F  VVEA +QVVSG+ YYL ++ + R
Sbjct:   30 AAAPAAPVLVGGRTEIRDVGSNKAVQSLGRFAVAEHN-RRLRHGGSGGPADPVPVKLAFARVVEAQKQVVSGVAYYLKVAASAR 112

[Close]

BLAST of FE670547 vs. SwissProt
Match: CYT4_ORYSJ (Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica GN=Os01g0915200 PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.149e-9
Identity = 31/68 (45.59%), Postives = 46/68 (67.65%), Query Frame = 1

Query:   37 VGAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGGEALKFVEVVEAHQQVVSGMKYYLNISTTD 240
            VG +TE+ D   +REV ELG+F+VEE+N ++  +   G   L+F  VV A +QVVSG+KYYL ++  +
Sbjct:   50 VGGRTEVRDVEGDREVQELGRFSVEEHNRRRR-SRDCGDVRLEFGRVVAAQRQVVSGLKYYLRVAAAE 116

[Close]

BLAST of FE670547 vs. SwissProt
Match: CYTB_HELAN (Cysteine proteinase inhibitor B OS=Helianthus annuus PE=1 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 4.113e-9
Identity = 28/70 (40.00%), Postives = 44/70 (62.86%), Query Frame = 1

Query:   40 GAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGGEALKFVEVVEAHQQVVSGMKYYLNISTTDRSG 249
            G +T++ + +T+ E+ +LG ++V+EYN  Q     G G+ LKF +V+ A  QVV+G KYYL I    + G
Sbjct:    3 GGRTKVKNVKTDTEIQQLGSYSVDEYNRLQRTKKTGAGD-LKFSQVIAAETQVVAGTKYYLKIEAITKGG 71

[Close]

BLAST of FE670547 vs. SwissProt
Match: CYT2_ARATH (Cysteine proteinase inhibitor 2 OS=Arabidopsis thaliana GN=CYS2 PE=2 SV=2)

HSP 1 Score: 58.9214 bits (141), Expect = 9.163e-9
Identity = 31/83 (37.35%), Postives = 48/83 (57.83%), Query Frame = 1

Query:   31 VLVGAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGG----------EALKFVEVVEAHQQVVSGMKYYLNISTTDRSG 249
            V++G K+ + + RTNRE+ +LG++ VE++N +     G  G            L+F  VV A +QVV+G+KYYL I  T  +G
Sbjct:   34 VVLGGKSGVPNIRTNREIQQLGRYCVEQFNQQAQNEQGNIGSIAKTDTAISNPLQFSRVVSAQKQVVAGLKYYLRIEVTQPNG 116

[Close]

BLAST of FE670547 vs. SwissProt
Match: CYT5_ORYSJ (Cysteine proteinase inhibitor 5 OS=Oryza sativa subsp. japonica GN=Os01g0915401 PE=3 SV=2)

HSP 1 Score: 58.5362 bits (140), Expect = 1.197e-8
Identity = 33/68 (48.53%), Postives = 41/68 (60.29%), Query Frame = 1

Query:   37 VGAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGGEALKFVEVVEAHQQVVSGMKYYLNISTTD 240
            VG +T++ D   NREV ELG F V E+N + G    G G  L F  VV A  QVVSG+KYYL I+  +
Sbjct:   49 VGGRTDVEDVEGNREVQELGLFCVVEHNRRGGSATRGRG--LVFSRVVAAQTQVVSGIKYYLRIAAQE 114

[Close]

BLAST of FE670547 vs. TrEMBL
Match: C6T5V2_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.194e-17
Identity = 54/81 (66.67%), Postives = 59/81 (72.84%), Query Frame = 1

Query:    7 LSTASCGRVLVGAKTEISDARTNREV*ELGKFAVEEYN--YKQGVNNGGGGEALKFVEVVEAHQQVVSGMKYYLNISTTDR 243
            LS+ASC R +VG KTE+ D RTNREV ELG+FAVEEYN   KQ  NN  G E L F EVVEA QQVVSGM YYL IS T +
Sbjct:   15 LSSASCAR-MVGGKTEVPDVRTNREVQELGRFAVEEYNRGLKQWKNN--GSEQLNFSEVVEAQQQVVSGMMYYLKISATHK 92

[Close]

BLAST of FE670547 vs. TrEMBL
Match: Q84XY6_MALDO (Cystatin OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.697e-14
Identity = 42/78 (53.85%), Postives = 55/78 (70.51%), Query Frame = 1

Query:    1 CFLSTASCGRV-LVGAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGGEALKFVEVVEAHQQVVSGMKYYLNIS 231
            C L  AS G   +VG + EI + +TN+EV ELG+F+VEEYN ++G     GG  L+F+EVVEA  QVVSG+KYYL +S
Sbjct:   12 CLLFVASNGYGGMVGGRKEIENVKTNKEVQELGRFSVEEYNRQRGTQKMDGGGELQFLEVVEAQSQVVSGIKYYLKVS 89

[Close]

BLAST of FE670547 vs. TrEMBL
Match: B9IAB9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_664681 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 8.012e-14
Identity = 39/73 (53.42%), Postives = 56/73 (76.71%), Query Frame = 1

Query:   34 LVGAKTEISDARTNREV*ELGKFAVEEYNYKQGVN-NGGGGEALKFVEVVEAHQQVVSGMKYYLNISTTDRSG 249
            +VG ++E+SD +TN++V ELG+F+V+E+N  + +   GGG   L F EVVEAH+QVVSG+KYYLNI  T ++G
Sbjct:   33 MVGGRSEVSDVKTNKQVQELGRFSVKEFNSHRSLYWKGGGVGKLMFSEVVEAHKQVVSGLKYYLNIVATTQNG 105

[Close]

BLAST of FE670547 vs. TrEMBL
Match: B9RRK6_RICCO (Cysteine proteinase inhibitor B, putative OS=Ricinus communis GN=RCOM_1645090 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.157e-12
Identity = 38/78 (48.72%), Postives = 54/78 (69.23%), Query Frame = 1

Query:   31 VLVGAKTEISDARTNREV*ELGKFAVEEYNYKQ-----GVNNGGGGEALKFVEVVEAHQQVVSGMKYYLNISTTDRSG 249
            ++VG +T++ D ++N  V ELG+F V+E+N +Q     G +NGGGGE L F EVV A  QVVSG+KYYL I+ ++  G
Sbjct:   25 MMVGGRTQVRDVKSNEAVQELGRFCVKEFNKQQLRQQHGGSNGGGGELLMFSEVVAAETQVVSGIKYYLKIAASNGKG 102

[Close]

BLAST of FE670547 vs. TrEMBL
Match: A5BLI6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_001398 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.279e-11
Identity = 42/82 (51.22%), Postives = 55/82 (67.07%), Query Frame = 1

Query:   16 ASCGRVLVGAKTEISDARTNREV*ELGKFAVEEYN------YKQGVNN--GGGGEALKFVEVVEAHQQVVSGMKYYLNISTT 237
            A  GR +VG +TEI D +TN+EV +LG+F+VEEYN      +++ + N  G   E L F EVVEA +QVVSG KYYL I+ T
Sbjct:   28 AGIGR-MVGGRTEIKDVKTNKEVQQLGRFSVEEYNRSHGRRWREAIGNVLGMDYEYLTFSEVVEAQKQVVSGFKYYLKIAAT 108

[Close]

BLAST of FE670547 vs. TrEMBL
Match: B9GNE2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_645343 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 4.861e-11
Identity = 36/75 (48.00%), Postives = 51/75 (68.00%), Query Frame = 1

Query:   25 GRVLVGAKTEISDARTNREV*ELGKFAVEEYN-YKQGVNNGGGGEALKFVEVVEAHQQVVSGMKYYLNISTTDRS 246
            G  +VG + ++SD +TN+E+ ELG+F+V+E+N ++     GG    L F EVVEA  QVVSG+KYYL I  T +S
Sbjct:   29 GGGMVGGRAKVSDVKTNKEIQELGRFSVKEFNNHRSTYGKGGEVGELMFSEVVEAQTQVVSGVKYYLKIEATTQS 103

[Close]

BLAST of FE670547 vs. TrEMBL
Match: Q4FZ47_MAIZE (Putative cystatin OS=Zea mays GN=cc10 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.847e-10
Identity = 36/71 (50.70%), Postives = 47/71 (66.20%), Query Frame = 1

Query:   37 VGAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGGEALKFVEVVEAHQQVVSGMKYYLNISTTDRSG 249
            VGA+TE+ D   + EV ELG+F+V EYN +Q    GGGG  L+F  VV A +QVVSG+KYYL +   +  G
Sbjct:   52 VGARTEVPDVEGDGEVQELGRFSVAEYN-RQLREGGGGGGRLEFGRVVAAQRQVVSGLKYYLRVVAVEEGG 121

[Close]

BLAST of FE670547 vs. TrEMBL
Match: Q4A1J1_MAIZE (Putative cystatin (Fragment) OS=Zea mays GN=cc10 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.847e-10
Identity = 35/71 (49.30%), Postives = 48/71 (67.61%), Query Frame = 1

Query:   37 VGAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGGEALKFVEVVEAHQQVVSGMKYYLNISTTDRSG 249
            VGA+TE+ D   + EV ELG+F+V EYN +  + +GGGG  L+F  VV A +QVVSG+KYYL +   +  G
Sbjct:   18 VGARTEVRDVEGDGEVQELGRFSVAEYNRQ--LRDGGGGGRLEFGRVVAAQRQVVSGLKYYLRVVAVEEGG 86

[Close]

BLAST of FE670547 vs. TrEMBL
Match: B6SIE7_MAIZE (Cysteine proteinase inhibitor B OS=Zea mays PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.847e-10
Identity = 36/71 (50.70%), Postives = 47/71 (66.20%), Query Frame = 1

Query:   37 VGAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGGEALKFVEVVEAHQQVVSGMKYYLNISTTDRSG 249
            VGA+TE+ D   + EV ELG+F+V EYN +Q    GGGG  L+F  VV A +QVVSG+KYYL +   +  G
Sbjct:   48 VGARTEVRDVEGDGEVQELGRFSVAEYN-RQLREGGGGGGRLEFGRVVAAQRQVVSGLKYYLRVVAVEEGG 117

[Close]

BLAST of FE670547 vs. TrEMBL
Match: O04430_DAUCA (Extracellular insoluble cystatin OS=Daucus carota GN=EICC PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 9.167e-10
Identity = 36/74 (48.65%), Postives = 51/74 (68.92%), Query Frame = 1

Query:   25 GRVLVGAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGGEA------LKFVEVVEAHQQVVSGMKYYLNI 228
            G   VG +TEI D  +N E+ +LG+++VE+YN  Q  +NG GG++      LKFV+VV A +QVV+G+KYYL I
Sbjct:   28 GSGAVGGRTEIPDVESNEEIQQLGEYSVEQYN--QQHHNGDGGDSTDSAGDLKFVKVVAAEKQVVAGIKYYLKI 99

[Close]

BLAST of FE670547 vs. TAIR peptide
Match: AT2G31980.1 (| Symbols: AtCYS2, CYS2 | PHYTOCYSTATIN 2 | chr2:13609246-13609770 REVERSE LENGTH=147)

HSP 1 Score: 58.9214 bits (141), Expect = 7.421e-10
Identity = 31/83 (37.35%), Postives = 48/83 (57.83%), Query Frame = 1

Query:   31 VLVGAKTEISDARTNREV*ELGKFAVEEYNYKQGVNNGGGG----------EALKFVEVVEAHQQVVSGMKYYLNISTTDRSG 249
            V++G K+ + + RTNRE+ +LG++ VE++N +     G  G            L+F  VV A +QVV+G+KYYL I  T  +G
Sbjct:   34 VVLGGKSGVPNIRTNREIQQLGRYCVEQFNQQAQNEQGNIGSIAKTDTAISNPLQFSRVVSAQKQVVAGLKYYLRIEVTQPNG 116

The following BLAST results are available for this feature:

BLAST of FE670547 vs. SwissProt
BLAST of FE670547 vs. TrEMBL
BLAST of FE670547 vs. TAIR peptide

BLAST of FE670547 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 6


Match Name	E-value	Identity	Description
CYT10_ORYSJ	8.287e-10	44.05	Cysteine proteinase inhibitor 10 OS=Oryza sativa s...	[more]
CYT10_ORYSI	8.287e-10	44.05	Cysteine proteinase inhibitor 10 OS=Oryza sativa s...	[more]
CYT4_ORYSJ	3.149e-9	45.59	Cysteine proteinase inhibitor 4 OS=Oryza sativa su...	[more]
CYTB_HELAN	4.113e-9	40.00	Cysteine proteinase inhibitor B OS=Helianthus annu...	[more]
CYT2_ARATH	9.163e-9	37.35	Cysteine proteinase inhibitor 2 OS=Arabidopsis tha...	[more]
CYT5_ORYSJ	1.197e-8	48.53	Cysteine proteinase inhibitor 5 OS=Oryza sativa su...	[more]

BLAST of FE670547 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T5V2_SOYBN	1.194e-17	66.67	Putative uncharacterized protein (Fragment) OS=Gly...	[more]
Q84XY6_MALDO	4.697e-14	53.85	Cystatin OS=Malus domestica PE=2 SV=1	[more]
B9IAB9_POPTR	8.012e-14	53.42	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RRK6_RICCO	1.157e-12	48.72	Cysteine proteinase inhibitor B, putative OS=Ricin...	[more]
A5BLI6_VITVI	1.279e-11	51.22	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9GNE2_POPTR	4.861e-11	48.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q4FZ47_MAIZE	1.847e-10	50.70	Putative cystatin OS=Zea mays GN=cc10 PE=2 SV=1	[more]
Q4A1J1_MAIZE	1.847e-10	49.30	Putative cystatin (Fragment) OS=Zea mays GN=cc10 P...	[more]
B6SIE7_MAIZE	1.847e-10	50.70	Cysteine proteinase inhibitor B OS=Zea mays PE=2 S...	[more]
O04430_DAUCA	9.167e-10	48.65	Extracellular insoluble cystatin OS=Daucus carota ...	[more]

BLAST of FE670547 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G31980.1	7.421e-10	37.35	\| Symbols: AtCYS2, CYS2 \| PHYTOCYSTATIN 2 \| chr2:1...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Proteinase inhibitor I25, cystatin	PFAM	PF00031	Cystatin	coord: 4..49 score: 8.4
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 35..48 score
None	No IPR available	GENE3D	3.10.450.10	no description	coord: 1..52 score: 7.1
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 1..51 score: 1.1
None	No IPR available	SEG	seg	seg	coord: 7..20 score
None	No IPR available	SEG	seg	seg	coord: 37..48 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE670547 ID=FE670547; Name=FE670547; organism=Cicer arietinum; type=EST; length=251bp
TGCTTCCTCTCAACGGCGTCGTGCGGACGCGTTTTAGTTGGGGCGAAAAC
AGAGATATCTGATGCAAGGACAAACAGGGAGGTATAGGAGCTTGGGAAAT
TCGCGGTGGAGGAGTATAATTACAAGCAAGGTGTAAACAACGGCGGCGGC
GGAGAGGCGTTGAAATTCGTTGAGGTTGTGGAGGCGCACCAGCAAGTGGT
GTCGGGGATGAAATACTATCTCAACATCTCAACAACTGATCGGAGTGGGA
G

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000010	Cystatin_dom
IPR018073	Prot_inh_cystat_CS

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