FE671395

EST Overview


Name	FE671395
Unique Name	FE671395
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	659

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FE671395 vs. SwissProt
Match: AMY1_HORVU (Alpha-amylase type A isozyme OS=Hordeum vulgare GN=AMY1.1 PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 9.538e-14
Identity = 33/55 (60.00%), Postives = 42/55 (76.36%), Query Frame = 2

Query:  410 LLFSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQVL 568
            ++F F T   L  AV+ E WRL DPQGK PGV+GWWP+++ TF+DNHDTGSTQ +
Sbjct:  268 MVFDFTTKGILNAAVEGELWRLIDPQGKAPGVMGWWPAKAATFVDNHDTGSTQAM 322

HSP 2 Score: 66.6254 bits (161), Expect = 1.289e-10
Identity = 33/74 (44.59%), Postives = 45/74 (60.81%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSIL-DYNQDSHRQRILNWIDGTGMLSTA---FDFTTKGILQVPI 215
            YSP+  K YI+G  P  +V E WD     G    +Y+QD+HRQ ++NW+D  G  ++A   FDFTTKGIL   +
Sbjct:  209 YSPEMAKVYIDGTSPSLAVAEVWDNMATGGDGKPNYDQDAHRQNLVNWVDKVGGAASAGMVFDFTTKGILNAAV 282

[Close]

BLAST of FE671395 vs. SwissProt
Match: AMYA_VIGMU (Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.246e-13
Identity = 36/71 (50.70%), Postives = 48/71 (67.61%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNY-KGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQVPI 215
            Y+P   K Y+E  KP F+VGE WD+ +Y +    +YNQDSHR  ++NW++  G   TAFDFTTKGILQ  +
Sbjct:  206 YAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQDSHRGALVNWVESAGGAITAFDFTTKGILQAAV 276

HSP 2 Score: 68.5514 bits (166), Expect = 3.393e-11
Identity = 35/53 (66.04%), Postives = 38/53 (71.70%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQVL 568
            F F T   LQ AV+ E WRL DP GKPPG+IG  P  +VTFIDNHDTGSTQ L
Sbjct:  264 FDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRL 316

[Close]

BLAST of FE671395 vs. SwissProt
Match: AMY2A_ORYSJ (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. japonica GN=AMY2A PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.625e-13
Identity = 33/53 (62.26%), Postives = 41/53 (77.36%), Query Frame = 2

Query:  410 LLFSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            ++F F T   +  AV+ E WRL D QGK PGVIGWWP+++VTF+DNHDTGSTQ
Sbjct:  267 MVFDFTTKGIMNTAVEGELWRLIDQQGKAPGVIGWWPAKAVTFVDNHDTGSTQ 319

HSP 2 Score: 67.781 bits (164), Expect = 5.787e-11
Identity = 34/75 (45.33%), Postives = 46/75 (61.33%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPL-FSVGEYWDTCNYKGSIL-DYNQDSHRQRILNWID---GTGMLSTAFDFTTKGILQVPI 215
            YSP+  K YIEG  P+  +V E WD+  Y G    +YNQD+HRQ +++W+D   GT      FDFTTKGI+   +
Sbjct:  207 YSPEVAKVYIEGTTPVGLAVAELWDSMAYGGDGKPEYNQDAHRQALVDWVDRVGGTASAGMVFDFTTKGIMNTAV 281

[Close]

BLAST of FE671395 vs. SwissProt
Match: AMY2A_ORYSI (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. indica GN=AMYC2 PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.625e-13
Identity = 33/53 (62.26%), Postives = 41/53 (77.36%), Query Frame = 2

Query:  410 LLFSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            ++F F T   +  AV+ E WRL D QGK PGVIGWWP+++VTF+DNHDTGSTQ
Sbjct:  267 MVFDFTTKGIMNTAVEGELWRLIDQQGKAPGVIGWWPAKAVTFVDNHDTGSTQ 319

HSP 2 Score: 67.781 bits (164), Expect = 5.787e-11
Identity = 34/75 (45.33%), Postives = 46/75 (61.33%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPL-FSVGEYWDTCNYKGSIL-DYNQDSHRQRILNWID---GTGMLSTAFDFTTKGILQVPI 215
            YSP+  K YIEG  P+  +V E WD+  Y G    +YNQD+HRQ +++W+D   GT      FDFTTKGI+   +
Sbjct:  207 YSPEVAKVYIEGTTPVGLAVAELWDSMAYGGDGKPEYNQDAHRQALVDWVDRVGGTASAGMVFDFTTKGIMNTAV 281

[Close]

BLAST of FE671395 vs. SwissProt
Match: AMY3A_ORYSJ (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 2.349e-12
Identity = 32/51 (62.75%), Postives = 39/51 (76.47%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQ AV+ E WR+RD  GK PG+IGW+P ++VTF+DNHDTGSTQ
Sbjct:  270 FDFTTKGILQSAVQGELWRMRDKDGKAPGMIGWYPEKAVTFVDNHDTGSTQ 320

HSP 2 Score: 65.0846 bits (157), Expect = 3.751e-10
Identity = 31/68 (45.59%), Postives = 42/68 (61.76%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDY-NQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            YS    + Y++ A+P F V E W++ +Y G      NQD  RQ ++NW+   G  +TAFDFTTKGILQ
Sbjct:  212 YSAAVARTYVQNARPSFVVAEIWNSLSYDGDGKPAANQDGQRQELVNWVKQVGGPATAFDFTTKGILQ 279

[Close]

BLAST of FE671395 vs. SwissProt
Match: AMY6_HORVU (Alpha-amylase type B isozyme OS=Hordeum vulgare GN=AMY1.6 PE=2 SV=2)

HSP 1 Score: 71.633 bits (174), Expect = 4.007e-12
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   L  AV+ E WRLR   GK PG+IGWWP+++VTF+DNHDTGSTQ
Sbjct:  270 FDFTTKGILNVAVEGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQ 320

HSP 2 Score: 60.8474 bits (146), Expect = 7.074e-9
Identity = 31/73 (42.47%), Postives = 44/73 (60.27%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSIL-DYNQDSHRQRILNWIDGTGML--STAFDFTTKGILQVPI 215
            YS    K Y++ ++P F+V E W +  Y G    + NQD HRQ ++NW++  G    +T FDFTTKGIL V +
Sbjct:  210 YSADVAKIYVDRSEPSFAVAEIWTSLAYGGDGKPNLNQDPHRQELVNWVNKVGGSGPATTFDFTTKGILNVAV 282

[Close]

BLAST of FE671395 vs. SwissProt
Match: AMY3_HORVU (Alpha-amylase type B isozyme (Fragment) OS=Hordeum vulgare GN=AMY1.3 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 4.007e-12
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   L  AV+ E WRLR   GK PG+IGWWP+++VTF+DNHDTGSTQ
Sbjct:  268 FDFTTKGILNVAVEGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQ 318

HSP 2 Score: 63.5438 bits (153), Expect = 1.091e-9
Identity = 33/73 (45.21%), Postives = 44/73 (60.27%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSIL-DYNQDSHRQRILNWIDGTGML--STAFDFTTKGILQVPI 215
            YS    K YI+ ++P F+V E W +  Y G    + NQD HRQ ++NW+D  G    +T FDFTTKGIL V +
Sbjct:  208 YSADVAKIYIDRSEPSFAVAEIWTSLAYGGDGKPNLNQDQHRQELVNWVDKVGGKGPATTFDFTTKGILNVAV 280

[Close]

BLAST of FE671395 vs. SwissProt
Match: AMY2_HORVU (Alpha-amylase type B isozyme OS=Hordeum vulgare GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 71.633 bits (174), Expect = 4.007e-12
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   L  AV+ E WRLR   GK PG+IGWWP+++VTF+DNHDTGSTQ
Sbjct:  268 FDFTTKGILNVAVEGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQ 318

HSP 2 Score: 63.5438 bits (153), Expect = 1.091e-9
Identity = 33/73 (45.21%), Postives = 44/73 (60.27%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSIL-DYNQDSHRQRILNWIDGTGML--STAFDFTTKGILQVPI 215
            YS    K YI+ ++P F+V E W +  Y G    + NQD HRQ ++NW+D  G    +T FDFTTKGIL V +
Sbjct:  208 YSADVAKIYIDRSEPSFAVAEIWTSLAYGGDGKPNLNQDQHRQELVNWVDKVGGKGPATTFDFTTKGILNVAV 280

[Close]

BLAST of FE671395 vs. SwissProt
Match: AMY1_ORYSJ (Alpha-amylase OS=Oryza sativa subsp. japonica GN=AMY1.1 PE=2 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 8.928e-12
Identity = 32/53 (60.38%), Postives = 38/53 (71.70%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQVL 568
            F F T   L  AV+ E WRLR   GK PG+IGWWP+++ TF+DNHDTGSTQ L
Sbjct:  275 FDFTTKGILNVAVEGELWRLRGEDGKAPGMIGWWPAKATTFVDNHDTGSTQHL 327

HSP 2 Score: 62.7734 bits (151), Expect = 1.862e-9
Identity = 32/74 (43.24%), Postives = 45/74 (60.81%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDT-CNYKGSILDYNQDSHRQRILNWID---GTGMLSTAFDFTTKGILQVPI 215
            YS    K YI+  +P F+V E W +  N      +Y+Q++HRQ ++NW+D   G    +TAFDFTTKGIL V +
Sbjct:  214 YSADMAKIYIDATEPSFAVAEIWTSMANGGDGKPNYDQNAHRQELVNWVDRVGGANSNATAFDFTTKGILNVAV 287

[Close]

BLAST of FE671395 vs. SwissProt
Match: AMY3E_ORYSJ (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.989e-11
Identity = 32/71 (45.07%), Postives = 43/71 (60.56%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSIL-DYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQVPI 215
            YS    + Y++   P F VGE W +  Y G      NQD+ RQ ++NW++G G  +TAFDFTTKGILQ  +
Sbjct:  209 YSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPATAFDFTTKGILQAAV 279

HSP 2 Score: 68.5514 bits (166), Expect = 3.393e-11
Identity = 32/53 (60.38%), Postives = 38/53 (71.70%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQVL 568
            F F T   LQ AV+ E WRL D  GK PG++GW P ++VTF+DNHDTGSTQ L
Sbjct:  267 FDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSL 319

[Close]

BLAST of FE671395 vs. TrEMBL
Match: C6TCX9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.477e-30
Identity = 61/67 (91.04%), Postives = 64/67 (95.52%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            +SPKYVKEYIEGAKPLF VGEYWD+CNYKGS LDYNQDSHRQRI+NWIDGTG LSTAFDFTTKGILQ
Sbjct:  198 FSPKYVKEYIEGAKPLFCVGEYWDSCNYKGSTLDYNQDSHRQRIINWIDGTGQLSTAFDFTTKGILQ 264

HSP 2 Score: 92.8189 bits (229), Expect = 2.447e-17
Identity = 42/51 (82.35%), Postives = 44/51 (86.27%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQEAVK  +WRLRDPQGKPPGVIGWWPSRSVTF+DNHDTGSTQ
Sbjct:  255 FDFTTKGILQEAVKGNFWRLRDPQGKPPGVIGWWPSRSVTFVDNHDTGSTQ 305

[Close]

BLAST of FE671395 vs. TrEMBL
Match: Q2VMU3_MANES (Alpha-amylase 2 OS=Manihot esculenta GN=amy2 PE=2 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.133e-29
Identity = 59/67 (88.06%), Postives = 63/67 (94.03%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            YSPKYVKEYIEGAKP+FSV EYWD+CNY GS L+YNQDSHRQRI+NWIDGTG LSTAFDFTTKGILQ
Sbjct:  193 YSPKYVKEYIEGAKPIFSVWEYWDSCNYSGSYLEYNQDSHRQRIINWIDGTGQLSTAFDFTTKGILQ 259

HSP 2 Score: 87.4261 bits (215), Expect = 1.028e-15
Identity = 40/51 (78.43%), Postives = 43/51 (84.31%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQEAVK ++WRLRD  GKPPGVIGWWPSR+VTFIDNHDTGSTQ
Sbjct:  250 FDFTTKGILQEAVKGQFWRLRDSPGKPPGVIGWWPSRAVTFIDNHDTGSTQ 300

[Close]

BLAST of FE671395 vs. TrEMBL
Match: Q5ZFR9_PLAMJ (Alpha-amylase OS=Plantago major GN=aamy1 PE=2 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 4.753e-29
Identity = 57/67 (85.07%), Postives = 62/67 (92.54%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            YSPKYVKEYIE AKP+FSVGEYWD+CNYKG  L+YNQDSHRQR++NWIDGTG LS AFDFTTKGILQ
Sbjct:  198 YSPKYVKEYIESAKPIFSVGEYWDSCNYKGQYLEYNQDSHRQRVINWIDGTGQLSAAFDFTTKGILQ 264

HSP 2 Score: 86.2705 bits (212), Expect = 2.290e-15
Identity = 39/51 (76.47%), Postives = 43/51 (84.31%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQ+AVK E WRLRD QGKPPGV+GWWPSR+VTF+DNHDTGSTQ
Sbjct:  255 FDFTTKGILQQAVKGELWRLRDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQ 305

[Close]

BLAST of FE671395 vs. TrEMBL
Match: Q1L5X0_NICLS (Alpha-amylase 1 large isoform (Fragment) OS=Nicotiana langsdorffii x Nicotiana sanderae GN=AAM1 PE=2 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 1.059e-28
Identity = 59/67 (88.06%), Postives = 62/67 (92.54%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            YSPKYVKEYIEGAKP+FSVGEYWDTCNYKGS LD NQDSHRQRI+NWID TG LS+AFDFTTK ILQ
Sbjct:  186 YSPKYVKEYIEGAKPIFSVGEYWDTCNYKGSYLDCNQDSHRQRIINWIDQTGQLSSAFDFTTKAILQ 252

HSP 2 Score: 88.5817 bits (218), Expect = 4.614e-16
Identity = 40/51 (78.43%), Postives = 44/51 (86.27%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQEAVK E+WRLRD +GKPPGV+GWWPSR+VTFIDNHDTGSTQ
Sbjct:  243 FDFTTKAILQEAVKGEFWRLRDSKGKPPGVLGWWPSRAVTFIDNHDTGSTQ 293

[Close]

BLAST of FE671395 vs. TrEMBL
Match: Q1L5W9_NICLS (Alpha-amylase 1 small isoform (Fragment) OS=Nicotiana langsdorffii x Nicotiana sanderae GN=AAM1 PE=2 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 1.059e-28
Identity = 59/67 (88.06%), Postives = 62/67 (92.54%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            YSPKYVKEYIEGAKP+FSVGEYWDTCNYKGS LD NQDSHRQRI+NWID TG LS+AFDFTTK ILQ
Sbjct:   56 YSPKYVKEYIEGAKPIFSVGEYWDTCNYKGSYLDCNQDSHRQRIINWIDQTGQLSSAFDFTTKAILQ 122

HSP 2 Score: 88.5817 bits (218), Expect = 4.614e-16
Identity = 40/51 (78.43%), Postives = 44/51 (86.27%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQEAVK E+WRLRD +GKPPGV+GWWPSR+VTFIDNHDTGSTQ
Sbjct:  113 FDFTTKAILQEAVKGEFWRLRDSKGKPPGVLGWWPSRAVTFIDNHDTGSTQ 163

[Close]

BLAST of FE671395 vs. TrEMBL
Match: Q41442_SOLTU (Alpha-amylase OS=Solanum tuberosum PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 4.023e-28
Identity = 56/67 (83.58%), Postives = 62/67 (92.54%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            Y+ KYVKEYIEGA+P+F+VGEYWDTCNYKGS LDYNQDSHRQRI+NWIDG G LSTAFDFTTK +LQ
Sbjct:  192 YASKYVKEYIEGAEPIFAVGEYWDTCNYKGSNLDYNQDSHRQRIINWIDGAGQLSTAFDFTTKAVLQ 258

HSP 2 Score: 83.5741 bits (205), Expect = 1.484e-14
Identity = 39/51 (76.47%), Postives = 43/51 (84.31%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQEAVK E+WRLRD +GKPPGV+G WPSR+VTFIDNHDTGSTQ
Sbjct:  249 FDFTTKAVLQEAVKGEFWRLRDSKGKPPGVLGLWPSRAVTFIDNHDTGSTQ 299

[Close]

BLAST of FE671395 vs. TrEMBL
Match: D1MEA8_SOLTU (Alpha-amylase OS=Solanum tuberosum GN=amyA2 PE=2 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 4.023e-28
Identity = 56/67 (83.58%), Postives = 62/67 (92.54%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            Y+ KYVKEYIEGA+P+F+VGEYWDTCNYKGS LDYNQDSHRQRI+NWIDG G LSTAFDFTTK +LQ
Sbjct:  192 YASKYVKEYIEGAEPIFAVGEYWDTCNYKGSNLDYNQDSHRQRIINWIDGAGQLSTAFDFTTKAVLQ 258

HSP 2 Score: 83.5741 bits (205), Expect = 1.484e-14
Identity = 39/51 (76.47%), Postives = 43/51 (84.31%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQEAVK E+WRLRD +GKPPGV+G WPSR+VTFIDNHDTGSTQ
Sbjct:  249 FDFTTKAVLQEAVKGEFWRLRDSKGKPPGVLGLWPSRAVTFIDNHDTGSTQ 299

[Close]

BLAST of FE671395 vs. TrEMBL
Match: C8CII0_SOLTU (Alpha-amylase OS=Solanum tuberosum GN=amyA1 PE=2 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 4.023e-28
Identity = 56/67 (83.58%), Postives = 62/67 (92.54%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            Y+ KYVKEYIEGA+P+F+VGEYWDTCNYKGS LDYNQDSHRQRI+NWIDG G LSTAFDFTTK +LQ
Sbjct:  192 YASKYVKEYIEGAEPIFAVGEYWDTCNYKGSNLDYNQDSHRQRIINWIDGAGQLSTAFDFTTKAVLQ 258

HSP 2 Score: 83.5741 bits (205), Expect = 1.484e-14
Identity = 39/51 (76.47%), Postives = 43/51 (84.31%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQEAVK E+WRLRD +GKPPGV+G WPSR+VTFIDNHDTGSTQ
Sbjct:  249 FDFTTKAVLQEAVKGEFWRLRDSKGKPPGVLGLWPSRAVTFIDNHDTGSTQ 299

[Close]

BLAST of FE671395 vs. TrEMBL
Match: B9GQ54_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_798380 PE=3 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 5.255e-28
Identity = 57/67 (85.07%), Postives = 61/67 (91.04%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            YSPKYVKEYIEGAKP+FSVGEYWD+CNY G  L+YNQDSHRQRI+NWID TG LS AFDFTTKGILQ
Sbjct:  191 YSPKYVKEYIEGAKPIFSVGEYWDSCNYNGHFLEYNQDSHRQRIVNWIDLTGQLSAAFDFTTKGILQ 257

HSP 2 Score: 92.0485 bits (227), Expect = 4.173e-17
Identity = 41/51 (80.39%), Postives = 45/51 (88.24%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQEAVK ++WRLRDPQGKPPGV+GWWPSR+VTFIDNHDTGSTQ
Sbjct:  248 FDFTTKGILQEAVKGQFWRLRDPQGKPPGVLGWWPSRAVTFIDNHDTGSTQ 298

[Close]

BLAST of FE671395 vs. TrEMBL
Match: Q5BLY0_MALDO (Cytosolic alpha-amylase OS=Malus domestica GN=AMY9 PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.997e-27
Identity = 57/67 (85.07%), Postives = 61/67 (91.04%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            YS KYVKEYIEGAKP+FSVGEYWD+CNY    LDYNQDSHRQRI+NWI+GTG LSTAFDFTTKGILQ
Sbjct:  199 YSAKYVKEYIEGAKPIFSVGEYWDSCNYNDHGLDYNQDSHRQRIVNWINGTGQLSTAFDFTTKGILQ 265

HSP 2 Score: 89.3521 bits (220), Expect = 2.705e-16
Identity = 40/51 (78.43%), Postives = 44/51 (86.27%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQEAVK + WRLRDPQGKPPG+IGWWPSR+VTF+DNHDTGSTQ
Sbjct:  256 FDFTTKGILQEAVKGQLWRLRDPQGKPPGLIGWWPSRAVTFLDNHDTGSTQ 306

[Close]

BLAST of FE671395 vs. TAIR peptide
Match: AT1G76130.1 (| Symbols: ATAMY2, AMY2 | alpha-amylase-like 2 | chr1:28561647-28563914 FORWARD LENGTH=413)

HSP 1 Score: 114.775 bits (286), Expect = 4.262e-26
Identity = 52/67 (77.61%), Postives = 56/67 (83.58%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQ 206
            YS  YVKEYI  AKPLFSVGE WD+CNY G  LDYNQDSHRQRI++WID TG +S AFDFTTKGILQ
Sbjct:  198 YSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTTKGILQ 264

HSP 2 Score: 86.2705 bits (212), Expect = 1.623e-17
Identity = 39/51 (76.47%), Postives = 43/51 (84.31%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            F F T   LQEAVK +YWRL D QGKPPGV+GWWPSR+VTF+DNHDTGSTQ
Sbjct:  255 FDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQ 305

[Close]

BLAST of FE671395 vs. TAIR peptide
Match: AT1G69830.1 (| Symbols: ATAMY3, AMY3 | alpha-amylase-like 3 | chr1:26288518-26293003 REVERSE LENGTH=887)

HSP 1 Score: 87.0409 bits (214), Expect = 9.517e-18
Identity = 37/69 (53.62%), Postives = 51/69 (73.91%), Query Frame = 3

Query:   18 YVKEYIEGAKPLFSVGEYWDTCNYKGSILDYNQDSHRQRILNWIDGTGMLSTAFDFTTKGILQVPIILC 224
            YVK+Y++ +KP F+VGEYWD+ +Y    +DYNQD+HRQRI++WI+ T   + AFD TTKGIL   +  C
Sbjct:  675 YVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKC 743

HSP 2 Score: 80.1073 bits (196), Expect = 1.163e-15
Identity = 32/38 (84.21%), Postives = 36/38 (94.74%), Query Frame = 2

Query:  449 KREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQ 562
            K EYWRL DP+GKPPGV+GWWPSR+VTFI+NHDTGSTQ
Sbjct:  742 KCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQ 779

[Close]

BLAST of FE671395 vs. TAIR peptide
Match: AT4G25000.1 (| Symbols: ATAMY1, AMY1 | alpha-amylase-like | chr4:12852109-12853825 REVERSE LENGTH=423)

HSP 1 Score: 66.2402 bits (160), Expect = 1.738e-11
Identity = 34/55 (61.82%), Postives = 37/55 (67.27%), Query Frame = 2

Query:  416 FSFCT--CLQEAVKREYWRLRDPQGKPPGVIGWWPSRSVTFIDNHDTGSTQVLSS 574
            F F T   LQ AVK E WRL+D QGKPPG+IG  P  +VTFIDNHDT  T V  S
Sbjct:  267 FDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRTWVFPS 321

HSP 2 Score: 65.0846 bits (157), Expect = 3.873e-11
Identity = 35/70 (50.00%), Postives = 44/70 (62.86%), Query Frame = 3

Query:    6 YSPKYVKEYIEGAKPLFSVGEYWDTCNYKG-SILDYNQDSHRQRILNWID--GTGMLSTAFDFTTKGILQ 206
            Y+    K Y++   P F+VGE WD   Y G   LDY+Q+ HR  +  WI+  G G+L TAFDFTTKGILQ
Sbjct:  208 YASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVL-TAFDFTTKGILQ 276

The following BLAST results are available for this feature:

BLAST of FE671395 vs. SwissProt
BLAST of FE671395 vs. TrEMBL
BLAST of FE671395 vs. TAIR peptide

BLAST of FE671395 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AMY1_HORVU	9.538e-14	60.00	Alpha-amylase type A isozyme OS=Hordeum vulgare GN...	[more]
AMYA_VIGMU	1.246e-13	50.70	Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1	[more]
AMY2A_ORYSJ	3.625e-13	62.26	Alpha-amylase isozyme 2A OS=Oryza sativa subsp. ja...	[more]
AMY2A_ORYSI	3.625e-13	62.26	Alpha-amylase isozyme 2A OS=Oryza sativa subsp. in...	[more]
AMY3A_ORYSJ	2.349e-12	62.75	Alpha-amylase isozyme 3A OS=Oryza sativa subsp. ja...	[more]
AMY6_HORVU	4.007e-12	62.75	Alpha-amylase type B isozyme OS=Hordeum vulgare GN...	[more]
AMY3_HORVU	4.007e-12	62.75	Alpha-amylase type B isozyme (Fragment) OS=Hordeum...	[more]
AMY2_HORVU	4.007e-12	62.75	Alpha-amylase type B isozyme OS=Hordeum vulgare GN...	[more]
AMY1_ORYSJ	8.928e-12	60.38	Alpha-amylase OS=Oryza sativa subsp. japonica GN=A...	[more]
AMY3E_ORYSJ	1.989e-11	45.07	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. ja...	[more]

BLAST of FE671395 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TCX9_SOYBN	1.477e-30	91.04	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q2VMU3_MANES	2.133e-29	88.06	Alpha-amylase 2 OS=Manihot esculenta GN=amy2 PE=2 ...	[more]
Q5ZFR9_PLAMJ	4.753e-29	85.07	Alpha-amylase OS=Plantago major GN=aamy1 PE=2 SV=1	[more]
Q1L5X0_NICLS	1.059e-28	88.06	Alpha-amylase 1 large isoform (Fragment) OS=Nicoti...	[more]
Q1L5W9_NICLS	1.059e-28	88.06	Alpha-amylase 1 small isoform (Fragment) OS=Nicoti...	[more]
Q41442_SOLTU	4.023e-28	83.58	Alpha-amylase OS=Solanum tuberosum PE=3 SV=1	[more]
D1MEA8_SOLTU	4.023e-28	83.58	Alpha-amylase OS=Solanum tuberosum GN=amyA2 PE=2 S...	[more]
C8CII0_SOLTU	4.023e-28	83.58	Alpha-amylase OS=Solanum tuberosum GN=amyA1 PE=2 S...	[more]
B9GQ54_POPTR	5.255e-28	85.07	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q5BLY0_MALDO	1.997e-27	85.07	Cytosolic alpha-amylase OS=Malus domestica GN=AMY9...	[more]

BLAST of FE671395 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G76130.1	4.262e-26	77.61	\| Symbols: ATAMY2, AMY2 \| alpha-amylase-like 2 \| c...	[more]
AT1G69830.1	9.517e-18	53.62	\| Symbols: ATAMY3, AMY3 \| alpha-amylase-like 3 \| c...	[more]
AT4G25000.1	1.738e-11	61.82	\| Symbols: ATAMY1, AMY1 \| alpha-amylase-like \| chr...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013781	Glycoside hydrolase, subgroup, catalytic core	GENE3D	3.20.20.80	no description	coord: 10..55 score: 3.2
IPR013781	Glycoside hydrolase, subgroup, catalytic core	GENE3D	3.20.20.80	no description	coord: 6..67 score: 0.0
None	No IPR available	PANTHER	PTHR10357	AMYLASE	coord: 16..58 score: 6.9
None	No IPR available	PANTHER	PTHR10357:SF9	ALPHA-AMYLASE	coord: 16..58 score: 6.9
None	No IPR available	PANTHER	PTHR10357	AMYLASE	coord: 2..68 score: 9.4
None	No IPR available	PANTHER	PTHR10357:SF9	ALPHA-AMYLASE	coord: 2..68 score: 9.4

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE671395 ID=FE671395; Name=FE671395; organism=Cicer arietinum; type=EST; length=659bp
ATTCCTATTCACCAAAATATGTGAAAGAATATATTGAAGGAGCAAAGCCA
TTGTTCTCTGTTGGTGAATATTGGGATACTTGCAACTACAAGGGTTCTAT
TTTGGACTATAACCAAGATAGCCATAGGCAACGAATACTCAATTGGATTG
ATGGCACTGGGATGCTTTCAACCGCATTTGACTTTACAACAAAGGGAATT
CTCCAGGTACCCATCATTTTATGCCTAGTATGTTAAATAAAGGTTTGAAA
AATGAGAATGGAAGTTAGATCTTACTGTGCATATATAAATACTTTTTTTC
CACTCCTTTGCTATTCAATGAGGTAGTGTCTATCTACGAACATTGATACT
AAATCTCAAGTGTATCGGTTATTTAATTCATACTATAAAATTACATTTCT
CACCACAAACTCCTTTTCTCATTTTGCACTTGTTTGCAGGAAGCTGTCAA
GCGAGAATATTGGCGTCTGCGTGATCCTCAAGGGAAGCCACCTGGTGTGA
TTGGGTGGTGGCCTTCACGATCAGTCACATTTATCGATAACCATGATACT
GGTTCAACTCAGGTGCTGAGTTCACTATTTGTNCAAATAAAATAATACAG
AGATGCTTCTTGAACAGTATTTGTAATTACCATTCTTGTTTCATGAAATG
CATGCTGGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0043169	cation binding

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR013781	Glyco_hydro_catalytic_dom

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