GR400519

EST Overview


Name	GR400519
Unique Name	GR400519
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	303

Libraries


Library Name	Type
LIBEST_024783 ICC4958 Slow drought stresses root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR400519 vs. SwissProt
Match: LOX3_SOYBN (Seed lipoxygenase-3 OS=Glycine max GN=LOX1.3 PE=1 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.079e-12
Identity = 34/59 (57.63%), Postives = 41/59 (69.49%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            + FK+FG KLA I  K+   N    ++ R GPVQ PY LL+PSS+EGLTFRGIP SI I
Sbjct:  799 EAFKRFGNKLAQIENKLSERNNDEKLRNRCGPVQMPYTLLLPSSKEGLTFRGIPNSISI 857

[Close]

BLAST of GR400519 vs. SwissProt
Match: LOX3_PEA (Seed lipoxygenase-3 OS=Pisum sativum GN=LOX1.3 PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 5.252e-12
Identity = 34/59 (57.63%), Postives = 41/59 (69.49%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            + FKKFG KLA+I  K+   N    ++ R GPV+ PY LL PSS+EGLTFRGIP SI I
Sbjct:  803 EAFKKFGNKLAEIEKKLTQRNNDEKLRNRHGPVEMPYTLLYPSSKEGLTFRGIPNSISI 861

[Close]

BLAST of GR400519 vs. SwissProt
Match: LOXX_SOYBN (Seed lipoxygenase OS=Glycine max GN=LOX1.4 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.958e-12
Identity = 34/59 (57.63%), Postives = 42/59 (71.19%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            + FK+FG+KL  I GKI   N+  +++ R GPVQ PY LL  SSEEGLTF+GIP SI I
Sbjct:  806 EAFKRFGSKLTGIEGKINARNSDPSLRNRTGPVQLPYTLLHRSSEEGLTFKGIPNSISI 864

[Close]

BLAST of GR400519 vs. SwissProt
Match: LOX2_PEA (Seed lipoxygenase-2 OS=Pisum sativum GN=LOX1.2 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.170e-11
Identity = 35/59 (59.32%), Postives = 41/59 (69.49%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            Q F+KFG KLA+I  K+   N   ++  R GPVQ PY LL PSS+EGLTFRGIP SI I
Sbjct:  806 QAFQKFGNKLAEIEAKLTNKNNDPSLYHRVGPVQLPYTLLHPSSKEGLTFRGIPNSISI 864

[Close]

BLAST of GR400519 vs. SwissProt
Match: LOXA_PHAVU (Lipoxygenase 1 OS=Phaseolus vulgaris GN=LOXA PE=3 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 3.404e-11
Identity = 34/60 (56.67%), Postives = 43/60 (71.67%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSE-EGLTFRGIPTSIFI 297
            Q FK+FG KL +I  KI G N  S+++ R GPV+ PY +L+P+ E EGLTFRGIP SI I
Sbjct:  803 QAFKRFGQKLKEIEEKIMGRNKDSSLRNRNGPVKMPYTVLLPTCEDEGLTFRGIPNSISI 862

[Close]

BLAST of GR400519 vs. SwissProt
Match: LOX1_LENCU (Lipoxygenase OS=Lens culinaris GN=LOX1.1 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 5.807e-11
Identity = 33/59 (55.93%), Postives = 41/59 (69.49%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            + FK+FG KLA+I  K+   N   +++ R GPV+ PY LL PSSEEGLT RGIP SI I
Sbjct:  808 EAFKRFGKKLAEIEEKLTQRNNDESLRNRYGPVKMPYTLLYPSSEEGLTCRGIPNSISI 866

[Close]

BLAST of GR400519 vs. SwissProt
Match: LOX1_SOYBN (Seed lipoxygenase-1 OS=Glycine max GN=LOX1.1 PE=1 SV=2)

HSP 1 Score: 65.855 bits (159), Expect = 7.584e-11
Identity = 37/60 (61.67%), Postives = 40/60 (66.67%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGK-IRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            Q F+KFG KL +I  K +R  N  S    R GPVQ PY LL PSSEEGLTFRGIP SI I
Sbjct:  780 QAFQKFGNKLKEIEEKLVRRNNDPSLQGNRLGPVQLPYTLLYPSSEEGLTFRGIPNSISI 839

[Close]

BLAST of GR400519 vs. SwissProt
Match: LOX4_SOYBN (Lipoxygenase-4 OS=Glycine max GN=LOX1.5 PE=1 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.207e-10
Identity = 32/59 (54.24%), Postives = 39/59 (66.10%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            + FK+FG KL +I  K+   N    ++ R GP + PY LL PSSEEGLTFRGIP SI I
Sbjct:  795 EAFKRFGKKLEEIEKKLIEKNKDETLRNRYGPAKMPYTLLYPSSEEGLTFRGIPNSISI 853

[Close]

BLAST of GR400519 vs. SwissProt
Match: LOXB_PHAVU (Lipoxygenase (Fragment) OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.186e-9
Identity = 29/53 (54.72%), Postives = 36/53 (67.92%), Query Frame = -1

Query:  139 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGI 297
            + FK+FG KLA+I  K+   N    ++ R GP + PY LL PSSEEGLTFRGI
Sbjct:  689 EAFKRFGKKLAEIEKKLVQKNNDETLRNRTGPAKMPYTLLYPSSEEGLTFRGI 741

[Close]

BLAST of GR400519 vs. SwissProt
Match: LOXB_SOLLC (Lipoxygenase B OS=Solanum lycopersicum GN=LOX1.2 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.435e-9
Identity = 29/57 (50.88%), Postives = 38/57 (66.67%), Query Frame = -1

Query:  121 FKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 291
            F++FG  L+DI  +I   N+  + K R GPV  PY LL P+SEEGLT +GIP S+ I
Sbjct:  803 FERFGKMLSDIENRIMIMNSHKSWKNRSGPVNVPYTLLFPTSEEGLTGKGIPNSVSI 859

[Close]

BLAST of GR400519 vs. TrEMBL
Match: Q9FEQ3_PEA (Lipoxygenase OS=Pisum sativum GN=lox1:Ps:7 PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.377e-12
Identity = 37/59 (62.71%), Postives = 44/59 (74.58%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            Q F KFGTKLA+I GKI   N  S++K R GPVQ PY LL+ +S+EGLTF+GIP SI I
Sbjct:  807 QAFTKFGTKLAEIEGKIHSRNKDSSLKNRYGPVQLPYSLLVRTSKEGLTFKGIPNSISI 865

[Close]

BLAST of GR400519 vs. TrEMBL
Match: Q9M3Z5_CICAR (Lipoxygenase (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.825e-12
Identity = 37/59 (62.71%), Postives = 44/59 (74.58%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            Q F+KFG+KL +I GKI   N  S++K R GP+Q PY LL+ SSEEGLTFRGIP SI I
Sbjct:  482 QAFQKFGSKLTEIEGKIIERNKDSSLKNRYGPIQLPYNLLLRSSEEGLTFRGIPNSISI 540

[Close]

BLAST of GR400519 vs. TrEMBL
Match: A7LCD5_SOYBN (Lipoxygenase OS=Glycine max GN=LOX9 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.283e-11
Identity = 35/59 (59.32%), Postives = 42/59 (71.19%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            Q FKKFG KL +I  KI G N  S+++ R GP Q PY +L+P+S EGLTFRGIP SI I
Sbjct:  807 QAFKKFGNKLKEIEDKISGRNKNSSLRNRNGPAQMPYTVLLPTSGEGLTFRGIPNSISI 865

[Close]

BLAST of GR400519 vs. TrEMBL
Match: O24320_PHAVU (Lipoxygenase OS=Phaseolus vulgaris PE=2 SV=2)

HSP 1 Score: 71.633 bits (174), Expect = 2.859e-11
Identity = 34/59 (57.63%), Postives = 44/59 (74.58%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            Q F+KFG KL +I  KI G N  S+++ R GPV+ PY +L+P+S+EGLTFRGIP SI I
Sbjct:  807 QAFQKFGNKLKEIENKILGRNNNSSLRNRVGPVKMPYTVLLPTSKEGLTFRGIPNSISI 865

[Close]

BLAST of GR400519 vs. TrEMBL
Match: O24295_PEA (Lipoxygenase OS=Pisum sativum GN=lox1:Ps:5 PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.859e-11
Identity = 36/59 (61.02%), Postives = 41/59 (69.49%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            Q FKKFG KLA I G I+  N  S++K R GPVQ PY +L PS E+GL FRGIP SI I
Sbjct:  808 QAFKKFGNKLAKIEGTIKERNNDSSLKNRYGPVQLPYTILQPSGEDGLAFRGIPNSISI 866

[Close]

BLAST of GR400519 vs. TrEMBL
Match: D6PAW7_SOYBN (Lipoxygenase OS=Glycine max GN=Lx3 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.368e-11
Identity = 34/59 (57.63%), Postives = 41/59 (69.49%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            + FK+FG KLA I  K+   N    ++ R GPVQ PY LL+PSS+EGLTFRGIP SI I
Sbjct:  799 EAFKRFGNKLAQIENKLSERNNDEKLRNRCGPVQMPYTLLLPSSKEGLTFRGIPNSISI 857

[Close]

BLAST of GR400519 vs. TrEMBL
Match: B3TDK6_SOYBN (Lipoxygenase OS=Glycine max GN=Lx3 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.368e-11
Identity = 34/59 (57.63%), Postives = 41/59 (69.49%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            + FK+FG KLA I  K+   N    ++ R GPVQ PY LL+PSS+EGLTFRGIP SI I
Sbjct:  799 EAFKRFGNKLAQIENKLSERNNDEKLRNRCGPVQMPYTLLLPSSKEGLTFRGIPNSISI 857

[Close]

BLAST of GR400519 vs. TrEMBL
Match: Q43440_SOYBN (Lipoxygenase OS=Glycine max GN=vlxC PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.317e-11
Identity = 35/59 (59.32%), Postives = 43/59 (72.88%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            + FKKFG KLA+I GKI   N   ++K+R GPVQ PY LL  SSEEG++F+GIP SI I
Sbjct:  801 EAFKKFGNKLAEIEGKITQRNNDPSLKSRHGPVQLPYTLLHRSSEEGMSFKGIPNSISI 859

[Close]

BLAST of GR400519 vs. TrEMBL
Match: O24470_PEA (Lipoxygenase OS=Pisum sativum GN=Lox1:Ps:1 PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.317e-11
Identity = 35/59 (59.32%), Postives = 40/59 (67.80%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            Q F KFGTKL +I GKI   N    ++ R GPVQ PY LL+ SSEEGLTFR IP S+ I
Sbjct:  810 QAFAKFGTKLTEIEGKIHSRNNEPGLRNRYGPVQLPYTLLLRSSEEGLTFRRIPNSVSI 868

[Close]

BLAST of GR400519 vs. TrEMBL
Match: Q41244_SOYBN (Lipoxygenase (Fragment) OS=Glycine max GN=loxA PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.853e-10
Identity = 34/59 (57.63%), Postives = 42/59 (71.19%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            + FK+FG+KL  I GKI   N+  +++ R GPVQ PY LL  SSEEGLTF+GIP SI I
Sbjct:  541 EAFKRFGSKLTGIEGKINARNSDPSLRNRTGPVQLPYTLLHRSSEEGLTFKGIPNSISI 599

[Close]

BLAST of GR400519 vs. TAIR peptide
Match: AT1G55020.1 (| Symbols: LOX1, ATLOX1 | lipoxygenase 1 | chr1:20525798-20530143 FORWARD LENGTH=859)

HSP 1 Score: 53.9138 bits (128), Expect = 2.351e-8
Identity = 28/59 (47.46%), Postives = 36/59 (61.02%), Query Frame = -1

Query:  121 QPFKKFGTKLADIGGKIRGGNTISNMKTRGGPVQFPYPLLIPSSEEGLTFRGIPTSIFI 297
            + F+KFG K+ +I   I   N    +K R G V+ PY LL PSSE G+T RGIP S+ I
Sbjct:  801 EAFEKFGEKVKEIEKNIDERNDDETLKNRTGLVKMPYTLLFPSSEGGVTGRGIPNSVSI 859

The following BLAST results are available for this feature:

BLAST of GR400519 vs. SwissProt
BLAST of GR400519 vs. TrEMBL
BLAST of GR400519 vs. TAIR peptide

BLAST of GR400519 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
LOX3_SOYBN	3.079e-12	57.63	Seed lipoxygenase-3 OS=Glycine max GN=LOX1.3 PE=1 ...	[more]
LOX3_PEA	5.252e-12	57.63	Seed lipoxygenase-3 OS=Pisum sativum GN=LOX1.3 PE=...	[more]
LOXX_SOYBN	8.958e-12	57.63	Seed lipoxygenase OS=Glycine max GN=LOX1.4 PE=1 SV...	[more]
LOX2_PEA	1.170e-11	59.32	Seed lipoxygenase-2 OS=Pisum sativum GN=LOX1.2 PE=...	[more]
LOXA_PHAVU	3.404e-11	56.67	Lipoxygenase 1 OS=Phaseolus vulgaris GN=LOXA PE=3 ...	[more]
LOX1_LENCU	5.807e-11	55.93	Lipoxygenase OS=Lens culinaris GN=LOX1.1 PE=2 SV=1	[more]
LOX1_SOYBN	7.584e-11	61.67	Seed lipoxygenase-1 OS=Glycine max GN=LOX1.1 PE=1 ...	[more]
LOX4_SOYBN	2.207e-10	54.24	Lipoxygenase-4 OS=Glycine max GN=LOX1.5 PE=1 SV=1	[more]
LOXB_PHAVU	3.186e-9	54.72	Lipoxygenase (Fragment) OS=Phaseolus vulgaris PE=2...	[more]
LOXB_SOLLC	5.435e-9	50.88	Lipoxygenase B OS=Solanum lycopersicum GN=LOX1.2 P...	[more]

BLAST of GR400519 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9FEQ3_PEA	3.377e-12	62.71	Lipoxygenase OS=Pisum sativum GN=lox1:Ps:7 PE=3 SV...	[more]
Q9M3Z5_CICAR	9.825e-12	62.71	Lipoxygenase (Fragment) OS=Cicer arietinum PE=2 SV...	[more]
A7LCD5_SOYBN	1.283e-11	59.32	Lipoxygenase OS=Glycine max GN=LOX9 PE=2 SV=1	[more]
O24320_PHAVU	2.859e-11	57.63	Lipoxygenase OS=Phaseolus vulgaris PE=2 SV=2	[more]
O24295_PEA	2.859e-11	61.02	Lipoxygenase OS=Pisum sativum GN=lox1:Ps:5 PE=3 SV...	[more]
D6PAW7_SOYBN	6.368e-11	57.63	Lipoxygenase OS=Glycine max GN=Lx3 PE=3 SV=1	[more]
B3TDK6_SOYBN	6.368e-11	57.63	Lipoxygenase OS=Glycine max GN=Lx3 PE=3 SV=1	[more]
Q43440_SOYBN	8.317e-11	59.32	Lipoxygenase OS=Glycine max GN=vlxC PE=2 SV=1	[more]
O24470_PEA	8.317e-11	59.32	Lipoxygenase OS=Pisum sativum GN=Lox1:Ps:1 PE=3 SV...	[more]
Q41244_SOYBN	1.853e-10	57.63	Lipoxygenase (Fragment) OS=Glycine max GN=loxA PE=...	[more]

BLAST of GR400519 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G55020.1	2.351e-8	47.46	\| Symbols: LOX1, ATLOX1 \| lipoxygenase 1 \| chr1:20...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000907	Lipoxygenase	PANTHER	PTHR11771	LIPOXYGENASE	coord: 5..61 score: 9.6
IPR013819	Lipoxygenase, C-terminal	PFAM	PF00305	Lipoxygenase	coord: 4..45 score: 1.3
IPR013819	Lipoxygenase, C-terminal	PROFILE	PS51393	LIPOXYGENASE_3	coord: 1..61 score: 19
IPR013819	Lipoxygenase, C-terminal	SUPERFAMILY	48484	Lipoxigenase	coord: 5..61 score: 1
None	No IPR available	GENE3D	1.20.245.10	no description	coord: 3..61 score: 2.9
None	No IPR available	PANTHER	PTHR11771:SF2	LIPOXYGENASE	coord: 5..61 score: 9.6

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR400519 ID=GR400519; Name=GR400519; organism=Cicer arietinum; type=EST; length=303bp
GCAGAAGAAGAGCACTAAGCAGCCAATTCCAAAAATACATTGGTACCAAC
ATACAAAAAATAATTGCCCATACCCCTAATTTGTGTTACTAAAAAGTAAA
GTGATTGAAAAGGTTCCTTAAATAAAGATACTGGTGGGAATTCCTCTGAA
GGTCAACCCTTCCTCACTTGAAGGAATGAGCAATGGGTATGGAAATTGAA
CTGGGCCACCCCGGGTTTTCATATTTGAAATGGTGTTTCCCCCCCTGATT
TTCCCCCCAATGTCCGCCAACTTGGTTCCAAACTTTTTGAAAGGTTGCCC
GCG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0046872	metal ion binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR000907	LipOase
IPR013819	LipOase_C

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