GR420643

EST Overview


Name	GR420643
Unique Name	GR420643
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	436

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28

Homology

[Close]

BLAST of GR420643 vs. SwissProt
Match: PHSL1_SOLTU (Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic OS=Solanum tuberosum PE=1 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 1.851e-18
Identity = 50/83 (60.24%), Postives = 56/83 (67.47%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            NYD+LIGSLEGNEGFGR      GKDFP      SYIECQ   DEAYRDQ++WT +   +   S+KFSSDRTIHE     WNI
Sbjct:  884 NYDDLIGSLEGNEGFGRADYFLVGKDFP------SYIECQEKVDEAYRDQKRWTTMSILNTAGSYKFSSDRTIHEYAKDIWNI 960

[Close]

BLAST of GR420643 vs. SwissProt
Match: PHSL_IPOBA (Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Ipomoea batatas PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.200e-17
Identity = 49/87 (56.32%), Postives = 56/87 (64.37%), Query Frame = 1

Query:   13 YDELIGSLEGNEGFGRG------KDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNIMHEIY 240
            YDEL+GSLEGNEGFGRG      KDFP      SYIECQ   DEAYRDQ+ WT +   +   S+KFSSDRTIHE     WNI   ++
Sbjct:  874 YDELLGSLEGNEGFGRGDYFLVGKDFP------SYIECQEKVDEAYRDQKIWTRMSILNTAGSYKFSSDRTIHEYAKDIWNIQPVVF 954

[Close]

BLAST of GR420643 vs. SwissProt
Match: PHSL_VICFA (Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Vicia faba GN=PHO1 PE=2 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 1.567e-17
Identity = 49/83 (59.04%), Postives = 55/83 (66.27%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            NYDELIGSLEGNEGFGR      G+DFP      SY+ECQ   D+AYRDQ+KWT +   +   S KFSSDRTIHE     WNI
Sbjct:  921 NYDELIGSLEGNEGFGRADYFLVGQDFP------SYLECQEEVDKAYRDQKKWTRMSILNTAGSSKFSSDRTIHEYAREIWNI 997

[Close]

BLAST of GR420643 vs. SwissProt
Match: PHSL2_SOLTU (Alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic OS=Solanum tuberosum GN=STP-1 PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.567e-17
Identity = 45/76 (59.21%), Postives = 54/76 (71.05%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHEW 219
            NY+EL+GSLEGNEG+GR      GKDFP       YIECQ   DEAYRDQ+KWT++   +   SFKFSSDRTIH++
Sbjct:  892 NYEELMGSLEGNEGYGRADYFLVGKDFPD------YIECQDKVDEAYRDQKKWTKMSILNTAGSFKFSSDRTIHQY 961

[Close]

BLAST of GR420643 vs. SwissProt
Match: PHSH_WHEAT (Alpha-glucan phosphorylase, H isozyme OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.423e-10
Identity = 33/70 (47.14%), Postives = 44/70 (62.86%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGRGKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHEW 219
            +Y  L+ SLEGN GFGRG  F       SYI+ Q   DEAY+D++KW ++   +   S KFSSDRTI ++
Sbjct:  750 DYTPLLDSLEGNTGFGRGDYFLVGYDFPSYIDAQARVDEAYKDKKKWVKMSILNTAGSGKFSSDRTIDQY 819

[Close]

BLAST of GR420643 vs. SwissProt
Match: PHSH_VICFA (Alpha-glucan phosphorylase, H isozyme OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 3.164e-10
Identity = 35/77 (45.45%), Postives = 47/77 (61.04%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGRGKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            +Y+ L+ SLEGN G+GRG  F       SY++ Q   DEAYRD+++W ++   S   S KFSSDRTI +     WNI
Sbjct:  760 DYNPLLDSLEGNSGYGRGDYFLVGYDFPSYMDAQEKVDEAYRDKKRWLKMSILSTAGSGKFSSDRTIAQYAKEIWNI 836

[Close]

BLAST of GR420643 vs. SwissProt
Match: PHSH_SOLTU (Alpha-glucan phosphorylase, H isozyme OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 9.206e-10
Identity = 34/77 (44.16%), Postives = 47/77 (61.04%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGRGKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            +Y+ L+ SLEGN G+GRG  F       SY++ Q   DEAY+D+++W ++   S   S KFSSDRTI +     WNI
Sbjct:  756 DYNPLLESLEGNSGYGRGDYFLVGHDFPSYMDAQARVDEAYKDRKRWIKMSILSTSGSGKFSSDRTISQYAKEIWNI 832

[Close]

BLAST of GR420643 vs. SwissProt
Match: PHSH_ARATH (Alpha-glucan phosphorylase, H isozyme OS=Arabidopsis thaliana GN=At3g46970 PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.202e-9
Identity = 35/77 (45.45%), Postives = 45/77 (58.44%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGRGKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            +Y  L+ SLEGN GFGRG  F       SY++ Q   DEAY+D++ W ++   S   S KFSSDRTI +     WNI
Sbjct:  759 DYGPLLDSLEGNTGFGRGDYFLVGYDFPSYMDAQAKVDEAYKDRKGWLKMSILSTAGSGKFSSDRTIAQYAKEIWNI 835

[Close]

BLAST of GR420643 vs. TrEMBL
Match: Q9LIB2_ARATH (Phosphorylase OS=Arabidopsis thaliana GN=At3g29320 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.531e-17
Identity = 51/83 (61.45%), Postives = 56/83 (67.47%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            +YDELIGSLEGNEGFGR      GKDFP      SYIECQ   DEAYRDQ++WT +   +   SFKFSSDRTIHE     WNI
Sbjct:  880 SYDELIGSLEGNEGFGRADYFLVGKDFP------SYIECQEKVDEAYRDQKRWTRMSIMNTAGSFKFSSDRTIHEYAKDIWNI 956

[Close]

BLAST of GR420643 vs. TrEMBL
Match: Q56YE5_ARATH (Alpha-glucan phosphorylase (Fragment) OS=Arabidopsis thaliana GN=At3g29320 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.531e-17
Identity = 51/83 (61.45%), Postives = 56/83 (67.47%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            +YDELIGSLEGNEGFGR      GKDFP      SYIECQ   DEAYRDQ++WT +   +   SFKFSSDRTIHE     WNI
Sbjct:   66 SYDELIGSLEGNEGFGRADYFLVGKDFP------SYIECQEKVDEAYRDQKRWTRMSIMNTAGSFKFSSDRTIHEYAKDIWNI 142

[Close]

BLAST of GR420643 vs. TrEMBL
Match: D7LNX4_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_323006 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.999e-17
Identity = 51/83 (61.45%), Postives = 56/83 (67.47%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            +YDELIGSLEGNEGFGR      GKDFP      SYIECQ   DEAYRDQ++WT +   +   SFKFSSDRTIHE     WNI
Sbjct:  867 SYDELIGSLEGNEGFGRADYFLVGKDFP------SYIECQEKVDEAYRDQKRWTRMSILNTAGSFKFSSDRTIHEYAKDIWNI 943

[Close]

BLAST of GR420643 vs. TrEMBL
Match: C6TI30_SOYBN (Phosphorylase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 9.921e-17
Identity = 50/83 (60.24%), Postives = 55/83 (66.27%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            NYDEL+GSLEGNEGFGR      GKDFP      SYIECQ   DEAYR+Q KWT +   +   S+KFSSDRTIHE     WNI
Sbjct:  195 NYDELMGSLEGNEGFGRADYFLVGKDFP------SYIECQEKVDEAYRNQTKWTRMSILNTAGSYKFSSDRTIHEYAREIWNI 271

[Close]

BLAST of GR420643 vs. TrEMBL
Match: O24363_SPIOL (Phosphorylase OS=Spinacia oleracea GN=SoSTPII PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.296e-16
Identity = 48/83 (57.83%), Postives = 56/83 (67.47%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            +YDEL+GSLEGNEGFGR      GKDFP      SY+ECQ   D+AYRDQ+KWT +   +   SFKFSSDRTIH+     WNI
Sbjct:  889 SYDELLGSLEGNEGFGRADYFLVGKDFP------SYVECQEQVDQAYRDQQKWTRMSILNTAGSFKFSSDRTIHQYAKDIWNI 965

[Close]

BLAST of GR420643 vs. TrEMBL
Match: D7T9U5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00012194001 PE=4 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.296e-16
Identity = 46/76 (60.53%), Postives = 55/76 (72.37%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHEW 219
            NY+EL+GSLEGNEG+GR      GKDFP      SYIECQ   DEAYRDQ+KWT++   +   S+KFSSDRTIHE+
Sbjct:  851 NYEELMGSLEGNEGYGRADYFLVGKDFP------SYIECQEKVDEAYRDQKKWTKMSILNTAGSYKFSSDRTIHEY 920

[Close]

BLAST of GR420643 vs. TrEMBL
Match: B9H0D3_POPTR (Phosphorylase OS=Populus trichocarpa GN=POPTRDRAFT_712800 PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.692e-16
Identity = 49/83 (59.04%), Postives = 55/83 (66.27%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            NYDELIGSLEGNEGFGR      GKDFP      SYIECQ   D+AY DQ+ WT++   +   S+KFSSDRTIHE     WNI
Sbjct:  867 NYDELIGSLEGNEGFGRADYFLVGKDFP------SYIECQEEVDKAYHDQKTWTKMSIMNTAGSYKFSSDRTIHEYAREIWNI 943

[Close]

BLAST of GR420643 vs. TrEMBL
Match: D7SXJ4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_108.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011504001 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.210e-16
Identity = 49/83 (59.04%), Postives = 55/83 (66.27%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            NYDELIGSLEGNEGFG+      GKDFP      SYIECQ   DEAY DQ++WT +   +   S+KFSSDRTIHE     WNI
Sbjct:  900 NYDELIGSLEGNEGFGQADYFLVGKDFP------SYIECQEKVDEAYGDQKRWTRMSILNAAGSYKFSSDRTIHEYAKDIWNI 976

[Close]

BLAST of GR420643 vs. TrEMBL
Match: B9RCW0_RICCO (Phosphorylase OS=Ricinus communis GN=RCOM_1621440 PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 3.770e-16
Identity = 46/76 (60.53%), Postives = 53/76 (69.74%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHEW 219
            NYDEL+GSLEGNEG+GR      GKDFP      SY+ECQ   DEAYRDQ+KWT +   +   SFKFSSDRTI E+
Sbjct:  891 NYDELMGSLEGNEGYGRADYFLVGKDFP------SYLECQEKVDEAYRDQKKWTRMSILNTAGSFKFSSDRTIREY 960

[Close]

BLAST of GR420643 vs. TrEMBL
Match: B9HXL0_POPTR (Phosphorylase OS=Populus trichocarpa GN=POPTRDRAFT_881100 PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 8.398e-16
Identity = 43/76 (56.58%), Postives = 55/76 (72.37%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHEW 219
            NY+EL+GSLEGNEG+GR      GKDFP      SY+ECQ   DEAY+DQ++WT++   +   S+KFSSDRTIHE+
Sbjct:  871 NYEELMGSLEGNEGYGRADYFLVGKDFP------SYVECQEKVDEAYKDQKRWTKMSILNTAGSYKFSSDRTIHEY 940

[Close]

BLAST of GR420643 vs. TAIR peptide
Match: AT3G29320.1 (| Symbols: | Glycosyl transferase, family 35 | chr3:11252871-11257587 FORWARD LENGTH=962)

HSP 1 Score: 92.4337 bits (228), Expect = 1.010e-19
Identity = 51/83 (61.45%), Postives = 56/83 (67.47%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGR------GKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            +YDELIGSLEGNEGFGR      GKDFP      SYIECQ   DEAYRDQ++WT +   +   SFKFSSDRTIHE     WNI
Sbjct:  880 SYDELIGSLEGNEGFGRADYFLVGKDFP------SYIECQEKVDEAYRDQKRWTRMSIMNTAGSFKFSSDRTIHEYAKDIWNI 956

[Close]

BLAST of GR420643 vs. TAIR peptide
Match: AT3G46970.1 (| Symbols: ATPHS2, PHS2 | alpha-glucan phosphorylase 2 | chr3:17301625-17306111 REVERSE LENGTH=841)

HSP 1 Score: 62.003 bits (149), Expect = 1.462e-10
Identity = 35/77 (45.45%), Postives = 45/77 (58.44%), Query Frame = 1

Query:   10 NYDELIGSLEGNEGFGRGKDFPQIIFLSSYIECQG*KDEAYRDQRKWTEIQQESILNSFKFSSDRTIHE-----WNI 225
            +Y  L+ SLEGN GFGRG  F       SY++ Q   DEAY+D++ W ++   S   S KFSSDRTI +     WNI
Sbjct:  759 DYGPLLDSLEGNTGFGRGDYFLVGYDFPSYMDAQAKVDEAYKDRKGWLKMSILSTAGSGKFSSDRTIAQYAKEIWNI 835

The following BLAST results are available for this feature:

BLAST of GR420643 vs. SwissProt
BLAST of GR420643 vs. TrEMBL
BLAST of GR420643 vs. TAIR peptide

BLAST of GR420643 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 8


Match Name	E-value	Identity	Description
PHSL1_SOLTU	1.851e-18	60.24	Alpha-1,4 glucan phosphorylase L-1 isozyme, chloro...	[more]
PHSL_IPOBA	1.200e-17	56.32	Alpha-1,4 glucan phosphorylase L isozyme, chloropl...	[more]
PHSL_VICFA	1.567e-17	59.04	Alpha-1,4 glucan phosphorylase L isozyme, chloropl...	[more]
PHSL2_SOLTU	1.567e-17	59.21	Alpha-1,4 glucan phosphorylase L-2 isozyme, chloro...	[more]
PHSH_WHEAT	2.423e-10	47.14	Alpha-glucan phosphorylase, H isozyme OS=Triticum ...	[more]
PHSH_VICFA	3.164e-10	45.45	Alpha-glucan phosphorylase, H isozyme OS=Vicia fab...	[more]
PHSH_SOLTU	9.206e-10	44.16	Alpha-glucan phosphorylase, H isozyme OS=Solanum t...	[more]
PHSH_ARATH	1.202e-9	45.45	Alpha-glucan phosphorylase, H isozyme OS=Arabidops...	[more]

BLAST of GR420643 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9LIB2_ARATH	1.531e-17	61.45	Phosphorylase OS=Arabidopsis thaliana GN=At3g29320...	[more]
Q56YE5_ARATH	1.531e-17	61.45	Alpha-glucan phosphorylase (Fragment) OS=Arabidops...	[more]
D7LNX4_ARALY	1.999e-17	61.45	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
C6TI30_SOYBN	9.921e-17	60.24	Phosphorylase OS=Glycine max PE=2 SV=1	[more]
O24363_SPIOL	1.296e-16	57.83	Phosphorylase OS=Spinacia oleracea GN=SoSTPII PE=2...	[more]
D7T9U5_VITVI	1.296e-16	60.53	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9H0D3_POPTR	1.692e-16	59.04	Phosphorylase OS=Populus trichocarpa GN=POPTRDRAFT...	[more]
D7SXJ4_VITVI	2.210e-16	59.04	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RCW0_RICCO	3.770e-16	60.53	Phosphorylase OS=Ricinus communis GN=RCOM_1621440 ...	[more]
B9HXL0_POPTR	8.398e-16	56.58	Phosphorylase OS=Populus trichocarpa GN=POPTRDRAFT...	[more]

BLAST of GR420643 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT3G29320.1	1.010e-19	61.45	\| Symbols: \| Glycosyl transferase, family 35 \| ch...	[more]
AT3G46970.1	1.462e-10	45.45	\| Symbols: ATPHS2, PHS2 \| alpha-glucan phosphoryla...	[more]

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR420643 ID=GR420643; Name=GR420643; organism=Cicer arietinum; type=EST; length=436bp
TTACGGGTCAACTATGATGAACTGATTGGATCCTTGGAGGGAAATGAAGG
TTTTGGGCGAGGCAAGGACTTCCCCCAGATTATTTTCTTGTCGAGCTACA
TAGAATGTCAGGGTTAGAAGGACGAAGCTTATCGCGACCAAAGAAAATGG
ACAGAAATACAGCAGGAGTCAATATTGAACTCGTTCAAGTTCAGCAGTGA
CCGTACCATTCATGAATGGAACATTATGCACGAGATATATAATTGAGCCA
GCCAGCAATAGAGGAGGGTATTTATAGCAGAGGTAATCTATACTATATCC
AGTTATATCGGTGCCACGTTCCCGTACTGAAAAATAAACAGAAACTGTAA
ATAATTGTAATTTGTCCGCGGGCCTGCGCTCAAAATAAGCCTGCATTTCT
GTAATATTGTATTGTATGTAGTATTAAAAAAAGAAA

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