HO062209

EST Overview


Name	HO062209
Unique Name	HO062209
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	656

Libraries


Library Name	Type
LIBEST_026408 Chickpea drought stressed cDNA SSH library AB1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO062209 vs. SwissProt
Match: DNLI3_SCHPO (DNA ligase 3 OS=Schizosaccharomyces pombe GN=adl1 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.778e-26
Identity = 55/122 (45.08%), Postives = 80/122 (65.57%), Query Frame = -2

Query:  236 MFQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYY---QTGE-TPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQ 589
            ++  +T  Y++VC+ MSGFSD FY E+ + +S +   S   P Y   +TG+ TP ++F PQ VWEI+GA  T SP + AA+GL+   RG+SIRFPRFI   SD+ PE+ ST + + +M+  Q
Sbjct:  650 VYNPDTGAYEAVCKCMSGFSDQFYKELTQKYSLESGNSSLKPIYNFCETGKVTPQIYFAPQEVWEIKGAQITSSPAYKAALGLIQDDRGLSIRFPRFIRVRSDKGPEDASTNSILADMYMKQ 771

HSP 2 Score: 29.6462 bits (65), Expect = 1.778e-26
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = -1

Query:  600 HGNGRKAGWYSP 635
            +GNGRKAGW+SP
Sbjct:  634 YGNGRKAGWFSP 645

[Close]

BLAST of HO062209 vs. SwissProt
Match: DNLI1_ARATH (DNA ligase 1 OS=Arabidopsis thaliana GN=At1g08130 PE=2 SV=2)

HSP 1 Score: 108.612 bits (270), Expect = 2.930e-23
Identity = 52/140 (37.14%), Postives = 83/140 (59.29%), Query Frame = -2

Query:  233 ACMEMEGKLDGIVHSH--GMFQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGET--PDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQK 640
            A     GK  G+  +     +  + EE+QS+C++ +GFSD+   E         + + K  YY+ G++  PD+WF P  VWE++ AD T+SPVH AA G+V P +GIS+RFPR +    D+ PEE +++  I +++ +QK
Sbjct:  639 AAFHGRGKRTGVYGAFLLACYDVDKEEFQSICKIGTGFSDAMLDERSSSLRSQVIATPKQ-YYRVGDSLNPDVWFEPTEVWEVKAADLTISPVHRAATGIVDPDKGISLRFPRLLRVREDKKPEEATSSEQIADLYQAQK 777

[Close]

BLAST of HO062209 vs. SwissProt
Match: DNLI_PICTO (DNA ligase OS=Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) GN=lig PE=3 SV=2)

HSP 1 Score: 107.842 bits (268), Expect = 4.998e-23
Identity = 48/122 (39.34%), Postives = 77/122 (63.11%), Query Frame = -2

Query:  221 FQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMD 586
            + S+ + +++VC++ SGF+D    EM     GDK++ KKP    +   PD W  P LV EIRGA+ TVSPVH  A+ ++    G+++RFPR I    D+ PE+ +T  +I+EM+ +QK+ ++
Sbjct:  463 YNSKDDTFETVCKLGSGFTDEMLSEMPRLL-GDKIVEKKPARVNSSMEPDHWIYPSLVLEIRGAEITVSPVHTCAMNIIEKGSGLALRFPRLIKPRDDKKPEDATTTNEIIEMYKAQKKVIE 583

[Close]

BLAST of HO062209 vs. SwissProt
Match: DNLI1_DROME (DNA ligase 1 OS=Drosophila melanogaster GN=CG5602 PE=1 SV=2)

HSP 1 Score: 103.219 bits (256), Expect = 1.231e-21
Identity = 52/119 (43.70%), Postives = 72/119 (60.50%), Query Frame = -2

Query:  236 FQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGET--PDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQ 586
            + +E EEYQS+C++ +GF+D       EF  G  V S    YY+   +  PD WF P  VWE++ AD ++SP+H AAIG+V   RGIS+RFPRFI    D+N E  + A  +  M+ SQ
Sbjct:  610 YDTENEEYQSICKIGTGFTDEDLQTHSEFL-GKHVTSAAKSYYRYDPSLEPDHWFEPVQVWEVKCADLSLSPIHRAAIGIVDGERGISLRFPRFIRIRDDKNSENATDANQVAHMYQSQ 727

[Close]

BLAST of HO062209 vs. SwissProt
Match: DNLI1_CAEEL (DNA ligase 1 OS=Caenorhabditis elegans GN=lig-1 PE=2 SV=2)

HSP 1 Score: 103.219 bits (256), Expect = 1.231e-21
Identity = 53/134 (39.55%), Postives = 81/134 (60.45%), Query Frame = -2

Query:  233 GKLDGIVHSH--GMFQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGET--PDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQK 622
            GK  G+   +  G +   TEEY+SVC++ +GF+D    E  +    DK + K P YYQ   T  PD  F P LV+E++ AD T+SP H AA GL    +GIS+RFPRF+    D+N ++ +++  ++EM+ +Q+
Sbjct:  488 GKRTGVYGGYLLGCYNPTTEEYESVCKIGTGFTDEDLAEQYKILQ-DKKIDKSPSYYQFDHTLKPDDTFSPYLVFEVKCADITISPRHKAASGLTDDGKGISLRFPRFLRIRDDKNSDDATSSEQVLEMYKNQE 620

[Close]

BLAST of HO062209 vs. SwissProt
Match: DNLI1_XENLA (DNA ligase 1 OS=Xenopus laevis GN=lig1 PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 1.608e-21
Identity = 51/136 (37.50%), Postives = 81/136 (59.56%), Query Frame = -2

Query:  230 EGKLDGIVHSH--GMFQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQ--TGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKR 625
            +GK  GI        +  E+EEYQ++C++ +GF+D   +E    F  D V+     YY+  +   PD WF P  VWE++ AD ++SPVH AA+GLV   +GIS+RFPRF+    D+ PEE + +  + +++  Q++
Sbjct:  921 KGKRTGIYGGFLLASYDEESEEYQTICKIGTGFTDDD-LEKHYNFLKDHVIENPRSYYRWDSATEPDHWFDPVQVWEVKCADLSISPVHKAALGLVEDQKGISLRFPRFLRIRDDKKPEESTNSFQVADLYRKQQQ 1055

[Close]

BLAST of HO062209 vs. SwissProt
Match: DNLI1_SCHPO (DNA ligase 1 OS=Schizosaccharomyces pombe GN=cdc17 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 3.960e-20
Identity = 52/137 (37.96%), Postives = 81/137 (59.12%), Query Frame = -2

Query:  206 GMFQSETEEYQSVCRVMSGFSD----SFYIEMKEFFSGDKVLSKKPPYYQTGETP----DMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDVTTEG 592
            G +  +TE  QS+C++ +GFS+    +FY ++K     D V+SKK  +Y   + P    D+WF P+ +WE+  AD ++SPV+ AAIG V   +GIS+RFPRFI    D++ E+ +T+  + E + SQ        EG
Sbjct:  630 GCYDPDTETVQSICKLGTGFSEEHLETFYNQLK-----DIVISKKKDFYAHSDVPAHQPDVWFEPKYLWEVLAADLSLSPVYKAAIGYVQEDKGISLRFPRFIRIREDKSWEDATTSEQVSEFYRSQVAYSQKEKEG 761

[Close]

BLAST of HO062209 vs. SwissProt
Match: DNLI1_DICDI (DNA ligase 1 OS=Dictyostelium discoideum GN=lig1 PE=3 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.966e-19
Identity = 44/122 (36.07%), Postives = 81/122 (66.39%), Query Frame = -2

Query:  233 FQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDK--VLSKKPPYYQTGET--PDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQK 586
            +    EE+Q++C++ +GFSD    ++  F S  K  ++++    ++  ++  PD+WF P  VWE+  AD ++SPVH AA G++ P++GI++RFPRFI    D++PE+ +++  +V+M+ +QK
Sbjct: 1059 YDENNEEFQTLCKIGTGFSDE---QLTTFTSQLKPHLINQPRNQFRFSDSIKPDVWFSPSCVWEVLAADLSISPVHTAASGILDPNKGIALRFPRFIRIRPDKSPEDATSSDQVVDMYQNQK 1177

[Close]

BLAST of HO062209 vs. SwissProt
Match: DNLI_SULAC (DNA ligase OS=Sulfolobus acidocaldarius GN=lig PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 3.353e-19
Identity = 54/139 (38.85%), Postives = 86/139 (61.87%), Query Frame = -2

Query:  209 GKLDGIVHSHGMFQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQ-KRKMDVTTE 622
            GKL  ++ +   +  + + + +VC+V SGFSD+   E+++  S  K L KK P  ++   PD+W  P+ V EI GA+ T+SP+H    G+V    G+SIRFPRFI    D++ EE +T  +I+EM+ S+ K+K++  TE
Sbjct:  462 GKLSSLLMA--AYDPDKDTFYTVCKVASGFSDAELEEVQKMLSEAK-LDKKDPRVESEIQPDIWVKPKYVAEIIGAEITLSPLHTCCKGVVSEEAGLSIRFPRFIRWRDDKSVEEATTPNEILEMYQSRLKKKVEDITE 597

[Close]

BLAST of HO062209 vs. SwissProt
Match: DNLI_THENV (DNA ligase OS=Thermoproteus neutrophilus (strain DSM 2338 / JCM 9278 / V24Sta) GN=lig PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 5.719e-19
Identity = 46/120 (38.33%), Postives = 76/120 (63.33%), Query Frame = -2

Query:  230 FQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSV-SDRNPEECSTAADIVEMFHSQKR 586
            +  +T+ + +VC+V SGF+D+   +M E     K+  + P       TPD+WF PQ+V E+ GA+ T+SP+H   +G V P  G++IRFPRF     +D++PE+ +T A++VE++  QK+
Sbjct:  461 YDPQTDMFYTVCKVGSGFTDADLKKMYEVLQPYKIPHRHPRVVSK-MTPDVWFTPQVVIEVIGAEITLSPLHTCCLGAVRPGVGLAIRFPRFTGRYRTDKSPEQATTPAEMVELYKRQKK 579

[Close]

BLAST of HO062209 vs. TrEMBL
Match: D7KUD3_ARALY (ATP dependent DNA ligase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_338905 PE=4 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 1.455e-59
Identity = 99/126 (78.57%), Postives = 116/126 (92.06%), Query Frame = -2

Query:  209 FQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDVTTE 586
            F  ETEE+QSVCRVMSGFSD+FYIEMKE +SGDK+L+KKPPYY+TGETPDMWF  ++VWEIRGADFTVSPVH AA+GLVHPSRGIS+RFPRFI  V+DRNPEECSTAADI EMFH+Q RK+++T++
Sbjct: 1287 FNPETEEFQSVCRVMSGFSDAFYIEMKELYSGDKILTKKPPYYRTGETPDMWFSTEVVWEIRGADFTVSPVHSAALGLVHPSRGISVRFPRFIRKVTDRNPEECSTAADIAEMFHAQTRKLNITSQ 1412

HSP 2 Score: 33.4982 bits (75), Expect = 1.455e-59
Identity = 12/12 (100.00%), Postives = 12/12 (100.00%), Query Frame = -1

Query:  600 HGNGRKAGWYSP 635
            HGNGRKAGWYSP
Sbjct: 1270 HGNGRKAGWYSP 1281

[Close]

BLAST of HO062209 vs. TrEMBL
Match: A5AGP3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041957 PE=4 SV=1)

HSP 1 Score: 217.238 bits (552), Expect = 5.559e-59
Identity = 99/124 (79.84%), Postives = 110/124 (88.71%), Query Frame = -2

Query:  215 FQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDVT 586
            +  +TEE+QSVCRVMSGFSD+FY EMKEFF+ DK+LSKKPPYYQT E PDMWF P+L+WEIRGADFTVSPVH AAIGLVHPSRGIS+RFPRFI  + DR PEECSTAADI +MFHSQ RKMDVT
Sbjct:  146 YNPDTEEFQSVCRVMSGFSDNFYKEMKEFFNEDKILSKKPPYYQTAELPDMWFTPELIWEIRGADFTVSPVHQAAIGLVHPSRGISVRFPRFIRPIMDRRPEECSTAADIADMFHSQTRKMDVT 269

HSP 2 Score: 35.8094 bits (81), Expect = 5.559e-59
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -1

Query:  597 HGNGRKAGWYSPF 635
            HGNGRKAGWYSPF
Sbjct:  129 HGNGRKAGWYSPF 141

[Close]

BLAST of HO062209 vs. TrEMBL
Match: B9H344_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_555902 PE=4 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 9.474e-59
Identity = 102/132 (77.27%), Postives = 115/132 (87.12%), Query Frame = -2

Query:  209 FQSETEEYQSVCRVMSGFSDSFYIE------MKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDVTTE 586
            +  ETEE+QSVCRVMSGFSD+FYIE      MKEFFSGD++L+KKPPYY+T E PDMWF P++VWEIRGADFT+SPVH AA+GLVH SRGISIRFPRFI S+SDRNPEECSTAADI EMF+SQ RKMDVT E
Sbjct:   95 YNPETEEFQSVCRVMSGFSDAFYIEVITELQMKEFFSGDRILAKKPPYYRTVEAPDMWFSPEVVWEIRGADFTISPVHQAAVGLVHQSRGISIRFPRFIHSISDRNPEECSTAADIAEMFNSQTRKMDVTAE 226

HSP 2 Score: 35.8094 bits (81), Expect = 9.474e-59
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -1

Query:  597 HGNGRKAGWYSPF 635
            HGNGRKAGWYSPF
Sbjct:   78 HGNGRKAGWYSPF 90

[Close]

BLAST of HO062209 vs. TrEMBL
Match: D7TSX4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_6.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00030942001 PE=4 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 2.693e-58
Identity = 98/124 (79.03%), Postives = 109/124 (87.90%), Query Frame = -2

Query:  215 FQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDVT 586
            +  +TEE+QSVCRVMSGFSD+FY EMKEFF+ DK+LSKKPPYYQT E PDMWF P+L+WEIRGADFTVSPVH AAIGLVHPSRGIS+RFPRFI  + DR PEECSTAADI +MFH Q RKMDVT
Sbjct: 1264 YNPDTEEFQSVCRVMSGFSDNFYKEMKEFFNEDKILSKKPPYYQTAELPDMWFTPELIWEIRGADFTVSPVHQAAIGLVHPSRGISVRFPRFIRPIMDRRPEECSTAADIADMFHFQTRKMDVT 1387

HSP 2 Score: 35.8094 bits (81), Expect = 2.693e-58
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -1

Query:  597 HGNGRKAGWYSPF 635
            HGNGRKAGWYSPF
Sbjct: 1247 HGNGRKAGWYSPF 1259

[Close]

BLAST of HO062209 vs. TrEMBL
Match: Q9C9M5_ARATH (DNA ligase OS=Arabidopsis thaliana GN=F4N21_14 PE=3 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 3.188e-56
Identity = 99/143 (69.23%), Postives = 116/143 (81.12%), Query Frame = -2

Query:  209 FQSETEEYQSVCRVMSGFSDSFYIE-----------------MKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDVTTE 586
            F  ETEE+QSVCRVMSGFSD+FYIE                 MKEF+S DK+L+KKPPYY+TGETPDMWF  ++VWEIRGADFTVSPVH A++GLVHPSRGIS+RFPRFIS V+DRNPEECSTA DI EMFH+Q RKM++T++
Sbjct: 1274 FNPETEEFQSVCRVMSGFSDAFYIEVMKSSTLTLLFNDETDGMKEFYSEDKILAKKPPYYRTGETPDMWFSAEVVWEIRGADFTVSPVHSASLGLVHPSRGISVRFPRFISKVTDRNPEECSTATDIAEMFHAQTRKMNITSQ 1416

HSP 2 Score: 33.4982 bits (75), Expect = 3.188e-56
Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = -1

Query:  597 HGNGRKAGWYSPF 635
            +GNGRKAGWYSPF
Sbjct: 1257 YGNGRKAGWYSPF 1269

[Close]

BLAST of HO062209 vs. TrEMBL
Match: B9SPY8_RICCO (DNA ligase OS=Ricinus communis GN=RCOM_0466830 PE=3 SV=1)

HSP 1 Score: 203.756 bits (517), Expect = 5.896e-55
Identity = 92/123 (74.80%), Postives = 108/123 (87.80%), Query Frame = -2

Query:  218 FQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDV 586
            +  ETEE+QSVCRVMSGFSD+FY EMKE+FSGD++L KKP YY+T E PD WF P++VWEIRGA+FTVSPVH AA+GLVHPSRGIS+RFPRFI SV+DRN EECSTA DI +MF+SQ RKMD+
Sbjct: 1234 YNPETEEFQSVCRVMSGFSDAFYTEMKEYFSGDRILLKKPHYYRTAEVPDKWFSPEVVWEIRGAEFTVSPVHQAAVGLVHPSRGISMRFPRFIRSVTDRNAEECSTAEDIADMFNSQTRKMDI 1356

HSP 2 Score: 35.8094 bits (81), Expect = 5.896e-55
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -1

Query:  597 HGNGRKAGWYSPF 635
            HGNGRKAGWYSPF
Sbjct: 1217 HGNGRKAGWYSPF 1229

[Close]

BLAST of HO062209 vs. TrEMBL
Match: Q0JKB6_ORYSJ (Os01g0685500 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os01g0685500 PE=4 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 6.954e-50
Identity = 84/127 (66.14%), Postives = 105/127 (82.68%), Query Frame = -2

Query:  206 FQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDVTTEG 586
            +  E EE+QSVCRVMSGFSD FY EMKEF+SGD++L KKP YY+T E P++WF  + VWEIRGAD T+SPVHHAAIGLVHPSRGIS+R PR+I S  DR+PE+CSTA D+  +F +Q RKM+V+++G
Sbjct:  187 YNPEYEEFQSVCRVMSGFSDEFYKEMKEFYSGDRILPKKPVYYKTDELPELWFSAEQVWEIRGADLTLSPVHHAAIGLVHPSRGISVRMPRYIRSRPDRSPEDCSTATDVASLFKAQTRKMEVSSDG 313

HSP 2 Score: 33.4982 bits (75), Expect = 6.954e-50
Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = -1

Query:  597 HGNGRKAGWYSPF 635
            +GNGRKAGWYSPF
Sbjct:  170 YGNGRKAGWYSPF 182

[Close]

BLAST of HO062209 vs. TrEMBL
Match: C5XGT3_SORBI (Putative uncharacterized protein Sb03g031440 OS=Sorghum bicolor GN=Sb03g031440 PE=4 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 2.326e-36
Identity = 68/102 (66.67%), Postives = 85/102 (83.33%), Query Frame = -2

Query:  206 MKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDVTTEG 511
            MKEF+SG+++L KKP YY+T E P++WF  + +WEIRGAD T+SPVHHAAIGLVHPSRGIS+R PR+I SV DR+PE+C TA DI  MF +Q RKMDV++EG
Sbjct:    1 MKEFYSGERILPKKPVYYKTDEQPELWFTAEQIWEIRGADLTLSPVHHAAIGLVHPSRGISVRMPRYIRSVPDRSPEDCGTATDIASMFKAQTRKMDVSSEG 102

[Close]

BLAST of HO062209 vs. TrEMBL
Match: B9EYN2_ORYSJ (DNA ligase OS=Oryza sativa subsp. japonica GN=OsJ_03041 PE=3 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 2.572e-35
Identity = 66/102 (64.71%), Postives = 85/102 (83.33%), Query Frame = -2

Query:  206 MKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDVTTEG 511
            MKEF+SGD++L KKP YY+T E P++WF  + VWEIRGAD T+SPVHHAAIGLVHPSRGIS+R PR+I S  DR+PE+CSTA D+  +F +Q RKM+V+++G
Sbjct: 1349 MKEFYSGDRILPKKPVYYKTDELPELWFSAEQVWEIRGADLTLSPVHHAAIGLVHPSRGISVRMPRYIRSRPDRSPEDCSTATDVASLFKAQTRKMEVSSDG 1450

[Close]

BLAST of HO062209 vs. TrEMBL
Match: B8A7M7_ORYSI (DNA ligase OS=Oryza sativa subsp. indica GN=OsI_03304 PE=3 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 2.572e-35
Identity = 66/102 (64.71%), Postives = 85/102 (83.33%), Query Frame = -2

Query:  206 MKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDVTTEG 511
            MKEF+SGD++L KKP YY+T E P++WF  + VWEIRGAD T+SPVHHAAIGLVHPSRGIS+R PR+I S  DR+PE+CSTA D+  +F +Q RKM+V+++G
Sbjct: 1120 MKEFYSGDRILPKKPVYYKTDELPELWFSAEQVWEIRGADLTLSPVHHAAIGLVHPSRGISVRMPRYIRSRPDRSPEDCSTATDVASLFKAQTRKMEVSSDG 1221

[Close]

BLAST of HO062209 vs. TAIR peptide
Match: AT1G66730.1 (| Symbols: AtLIG6, LIG6 | DNA LIGASE 6 | chr1:24884991-24891823 FORWARD LENGTH=1396)

HSP 1 Score: 221.09 bits (562), Expect = 1.346e-61
Identity = 99/126 (78.57%), Postives = 116/126 (92.06%), Query Frame = -2

Query:  209 FQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQKRKMDVTTE 586
            F  ETEE+QSVCRVMSGFSD+FYIEMKEF+S DK+L+KKPPYY+TGETPDMWF  ++VWEIRGADFTVSPVH A++GLVHPSRGIS+RFPRFIS V+DRNPEECSTA DI EMFH+Q RKM++T++
Sbjct: 1270 FNPETEEFQSVCRVMSGFSDAFYIEMKEFYSEDKILAKKPPYYRTGETPDMWFSAEVVWEIRGADFTVSPVHSASLGLVHPSRGISVRFPRFISKVTDRNPEECSTATDIAEMFHAQTRKMNITSQ 1395

HSP 2 Score: 33.4982 bits (75), Expect = 1.346e-61
Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = -1

Query:  597 HGNGRKAGWYSPF 635
            +GNGRKAGWYSPF
Sbjct: 1253 YGNGRKAGWYSPF 1265

[Close]

BLAST of HO062209 vs. TAIR peptide
Match: AT1G08130.1 (| Symbols: ATLIG1, LIG1 | DNA ligase 1 | chr1:2542913-2547815 REVERSE LENGTH=790)

HSP 1 Score: 108.612 bits (270), Expect = 3.030e-24
Identity = 52/140 (37.14%), Postives = 83/140 (59.29%), Query Frame = -2

Query:  233 ACMEMEGKLDGIVHSH--GMFQSETEEYQSVCRVMSGFSDSFYIEMKEFFSGDKVLSKKPPYYQTGET--PDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQK 640
            A     GK  G+  +     +  + EE+QS+C++ +GFSD+   E         + + K  YY+ G++  PD+WF P  VWE++ AD T+SPVH AA G+V P +GIS+RFPR +    D+ PEE +++  I +++ +QK
Sbjct:  639 AAFHGRGKRTGVYGAFLLACYDVDKEEFQSICKIGTGFSDAMLDERSSSLRSQVIATPKQ-YYRVGDSLNPDVWFEPTEVWEVKAADLTISPVHRAATGIVDPDKGISLRFPRLLRVREDKKPEEATSSEQIADLYQAQK 777

[Close]

BLAST of HO062209 vs. TAIR peptide
Match: AT1G49250.1 (| Symbols: | ATP-dependent DNA ligase | chr1:18220533-18224256 FORWARD LENGTH=657)

HSP 1 Score: 107.071 bits (266), Expect = 8.815e-24
Identity = 52/122 (42.62%), Postives = 78/122 (63.93%), Query Frame = -2

Query:  233 FQSETEEYQSVCRVMSGFSDSFYIEMKEFFSG--DKVLSKKPPYYQTGE--TPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFISSVSDRNPEECSTAADIVEMFHSQK 586
            +  + EE+QS+C++ + FSD   +E+++  S    KV++    YYQ      PD+W  P  VWE++ AD TVSPV+  AIG+V P +GIS+RFPR +    D+NPEE +T+  I EM+ +QK
Sbjct:  526 YDVDKEEFQSICKIGTEFSD---VELQDLSSSLCSKVIATPKQYYQVDNDLNPDVWLEPTEVWEVKAADLTVSPVYREAIGIVDPDKGISLRFPRLVRIRKDKNPEEATTSDQIAEMYQAQK 644

The following BLAST results are available for this feature:

BLAST of HO062209 vs. SwissProt
BLAST of HO062209 vs. TrEMBL
BLAST of HO062209 vs. TAIR peptide

BLAST of HO062209 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
DNLI3_SCHPO	1.778e-26	45.08	DNA ligase 3 OS=Schizosaccharomyces pombe GN=adl1 ...	[more]
DNLI1_ARATH	2.930e-23	37.14	DNA ligase 1 OS=Arabidopsis thaliana GN=At1g08130 ...	[more]
DNLI_PICTO	4.998e-23	39.34	DNA ligase OS=Picrophilus torridus (strain ATCC 70...	[more]
DNLI1_DROME	1.231e-21	43.70	DNA ligase 1 OS=Drosophila melanogaster GN=CG5602 ...	[more]
DNLI1_CAEEL	1.231e-21	39.55	DNA ligase 1 OS=Caenorhabditis elegans GN=lig-1 PE...	[more]
DNLI1_XENLA	1.608e-21	37.50	DNA ligase 1 OS=Xenopus laevis GN=lig1 PE=2 SV=1	[more]
DNLI1_SCHPO	3.960e-20	37.96	DNA ligase 1 OS=Schizosaccharomyces pombe GN=cdc17...	[more]
DNLI1_DICDI	1.966e-19	36.07	DNA ligase 1 OS=Dictyostelium discoideum GN=lig1 P...	[more]
DNLI_SULAC	3.353e-19	38.85	DNA ligase OS=Sulfolobus acidocaldarius GN=lig PE=...	[more]
DNLI_THENV	5.719e-19	38.33	DNA ligase OS=Thermoproteus neutrophilus (strain D...	[more]

BLAST of HO062209 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7KUD3_ARALY	1.455e-59	78.57	ATP dependent DNA ligase family protein OS=Arabido...	[more]
A5AGP3_VITVI	5.559e-59	79.84	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9H344_POPTR	9.474e-59	77.27	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TSX4_VITVI	2.693e-58	79.03	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q9C9M5_ARATH	3.188e-56	69.23	DNA ligase OS=Arabidopsis thaliana GN=F4N21_14 PE=...	[more]
B9SPY8_RICCO	5.896e-55	74.80	DNA ligase OS=Ricinus communis GN=RCOM_0466830 PE=...	[more]
Q0JKB6_ORYSJ	6.954e-50	66.14	Os01g0685500 protein (Fragment) OS=Oryza sativa su...	[more]
C5XGT3_SORBI	2.326e-36	66.67	Putative uncharacterized protein Sb03g031440 OS=So...	[more]
B9EYN2_ORYSJ	2.572e-35	64.71	DNA ligase OS=Oryza sativa subsp. japonica GN=OsJ_...	[more]
B8A7M7_ORYSI	2.572e-35	64.71	DNA ligase OS=Oryza sativa subsp. indica GN=OsI_03...	[more]

BLAST of HO062209 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G66730.1	1.346e-61	78.57	\| Symbols: AtLIG6, LIG6 \| DNA LIGASE 6 \| chr1:2488...	[more]
AT1G08130.1	3.030e-24	37.14	\| Symbols: ATLIG1, LIG1 \| DNA ligase 1 \| chr1:2542...	[more]
AT1G49250.1	8.815e-24	42.62	\| Symbols: \| ATP-dependent DNA ligase \| chr1:1822...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012309	DNA ligase, ATP-dependent, C-terminal	PFAM	PF04679	DNA_ligase_A_C	coord: 22..118 score: 2.5
IPR012340	Nucleic acid-binding, OB-fold	GENE3D	2.40.50.140	no description	coord: 22..141 score: 6.9
IPR016027	Nucleic acid-binding, OB-fold-like	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 22..141 score: 7.5
None	No IPR available	PANTHER	PTHR10459	ATP-DEPENDENT DNA LIGASE FAMILY	coord: 22..149 score: 4.9
None	No IPR available	PANTHER	PTHR10459:SF10	DNA LIGASE I	coord: 22..149 score: 4.9
None	No IPR available	SEG	seg	seg	coord: 62..77 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO062209 ID=HO062209; Name=HO062209; organism=Cicer arietinum; type=EST; length=656bp
GCAAGGGGAAACAAAGTTCTCTTATAGTGAACGGGCTTGACGTTAATCAT
GTTTCAAAGCTATCTGTCAACGAAGCTTACAAGCCAAAAAAGGAAGTTAA
ATACACTGTATGTACATACAAAACATTGAAACATCTTTAGTAAGCTGCTT
AAAAATCCTTTACATTTGGAACATAGTATGTACATAAAAATCAAACCAAC
ATTCAACCTTCAGTTGTGACATCCATCTTCCGTTTCTGCGAATGAAACAT
TTCAACTATATCGGCAGCTGTGCTACATTCTTCGGGGTTTCTGTCTGACA
CACTGCTAATAAACCTTGGAAATCTAATTGAGATGCCTCGAGATGGATGA
ACTAAACCAATGGCAGCATGATGAACTGGGGATACTGTGAAGTCTGCACC
TCTTATTTCCCAAACAAGTTGTGGACAAAACCACATGTCTGGTGTCTCTC
CTGTTTGATAATATGGTGGCTTTTTTGACAACACTTTGTCCCCAGAGAAG
AATTCTTTCATCTCTATGTAAAATGAGTCTGAAAACCCAGACATCACTCG
GCATACACTTTGATACTCCTCAGTTTCAGATTGAAACATGCCATGAGAAT
GGACTATACCATCCAGCTTTCCTTCCATTTCCATGCATGCACCAGTGGTA
CTAATC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003910	DNA ligase (ATP) activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006260	DNA replication
GO:0006281	DNA repair
GO:0006310	DNA recombination

Vocabulary: **INTERPRO**
Term	Definition
IPR012309	DNA_ligase_ATP-dep_C
IPR012340	NA-bd_OB-fold
IPR016027	Nucleic acid-binding, OB-fold-like

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