HO062872

EST Overview
NameHO062872
Unique NameHO062872
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length393
Libraries
Library NameType
LIBEST_026409 Chickpea drought stressed cDNA SSH library AB2cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of HO062872 vs. SwissProt
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 134.806 bits (338), Expect = 1.319e-31
Identity = 64/98 (65.31%), Postives = 77/98 (78.57%), Query Frame = -1 
Query:   16 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 309
            DP+ W         R + KKGE++K+IAFGMGRR CPGE LA+R ISMTLALLIQCFDWK  + D ID+AERDGF LTKL+PLKAMCK+RPV+N++ K
Sbjct:  402 DPELWTEATTFKPER-FEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVPLKAMCKSRPVINKVFK 498
[Close]
BLAST of HO062872 vs. SwissProt
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 7.506e-19
Identity = 43/97 (44.33%), Postives = 62/97 (63.92%), Query Frame = -1 
Query:   19 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 309
            DP +W+  P       + K+ E +K++AFG+GRR CPG  LA R + + L  LIQCF+W+R  +  +DM E  G  + K IPLKA+CK RP +++II
Sbjct:  406 DPNTWD-DPDSFKPERFEKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKII 501
[Close]
BLAST of HO062872 vs. SwissProt
Match: C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 77.411 bits (189), Expect = 2.499e-14
Identity = 36/100 (36.00%), Postives = 60/100 (60.00%), Query Frame = -1 
Query:   19 DPKSWE*RPQPSSRR---GWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 309
            DP+ WE   + +  R   G      + K++ FG GRR CPG  L  R +++ L  LIQCF+W+    + +DM+E  G  + K+ PL+AMC+ RP++++++
Sbjct:  400 DPEIWEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSKLL 499
[Close]
BLAST of HO062872 vs. SwissProt
Match: C93A2_SOYBN (Cytochrome P450 93A2 OS=Glycine max GN=CYP93A2 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.189e-10
Identity = 37/102 (36.27%), Postives = 56/102 (54.90%), Query Frame = -1 
Query:   28 DPKSWE*----RPQPSSRRGWTK---KGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWK-RTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVN 309
            DP  WE     RP+     G ++   +G+    I FG GRR CPG +LAL+ + + LA++IQCF WK    ++ +DM E+ G  L +  P+  +C   P +N
Sbjct:  398 DPNHWENPFEFRPERFFENGQSQLDVRGQHYHFIPFGSGRRSCPGTSLALQIVHVNLAIMIQCFQWKFDNGNNKVDMEEKSGITLPRAHPI--ICVPVPRLN 497
[Close]
BLAST of HO062872 vs. SwissProt
Match: C93A1_SOYBN (Cytochrome P450 93A1 OS=Glycine max GN=CYP93A1 PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 2.859e-10
Identity = 37/102 (36.27%), Postives = 54/102 (52.94%), Query Frame = -1 
Query:   28 DPKSWE*----RPQPSSRRGWTK---KGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRT-SDDMIDMAERDGFVLTKLIPLKAMCKTRPVVN 309
            DP  WE     RP+   R G  +   +G+    I FG GRR CPG +LA + + + LA++IQCF WK    +  +DM E+ G  L +  P+  +C   P +N
Sbjct:  405 DPNHWEKPFEFRPERFIRDGQNQLDVRGQHYHFIPFGSGRRTCPGASLAWQVVPVNLAIIIQCFQWKLVGGNGKVDMEEKSGITLPRANPI--ICVPVPRIN 504
[Close]
BLAST of HO062872 vs. SwissProt
Match: C93A3_SOYBN (Cytochrome P450 93A3 OS=Glycine max GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 6.369e-10
Identity = 34/91 (37.36%), Postives = 52/91 (57.14%), Query Frame = -1 
Query:   61 DPKSWE*----RPQPSSRRGWTK---KGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDD-MIDMAERDGFVLTKLIPL 309
            DP  WE     RP+     G ++   +G+   ++ FG GRR CPG +LAL+ + + LA+LIQCF WK   D+  ++M E+ G  L +  P+
Sbjct:  406 DPNHWENPLEFRPERFVENGKSQLDVRGQHYHLLPFGSGRRACPGTSLALQVVHVNLAVLIQCFQWKVDCDNGKVNMEEKAGITLPRAHPI 496
[Close]
BLAST of HO062872 vs. SwissProt
Match: C71AE_ARATH (Cytochrome P450 71A14 OS=Arabidopsis thaliana GN=CYP71A14 PE=2 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 3.648e-9
Identity = 25/67 (37.31%), Postives = 38/67 (56.72%), Query Frame = -1 
Query:   61 KGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWK---RTSDDMIDMAERDGFVLTKLIPL 252
            +G   + I FG GRR+CPG   A+  + +TLA L+  F+W+   + S D  D+AE  G  + +  PL
Sbjct:  424 RGTNFEFIPFGSGRRICPGIGFAMALVEVTLANLVNRFNWRMEVQHSGDEYDLAESTGLDVCRKFPL 490

HSP 2 Score: 30.8018 bits (68), Expect = 3.648e-9
Identity = 13/24 (54.17%), Postives = 16/24 (66.67%), Query Frame = -3 
Query:  263 INAWAIHRGS*VMGVKATTFKPER 334
            INAWAI R +   G+ A  F+PER
Sbjct:  392 INAWAIQRDTMTWGIDAEEFRPER 415
[Close]
BLAST of HO062872 vs. SwissProt
Match: C71AF_ARATH (Cytochrome P450 71A15 OS=Arabidopsis thaliana GN=CYP71A15 PE=2 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 6.134e-9
Identity = 26/67 (38.81%), Postives = 38/67 (56.72%), Query Frame = -1 
Query:   61 KGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWK---RTSDDMIDMAERDGFVLTKLIPL 252
            +G   + I FG GRR+CPG   A+  + +TLA L+  F+W+   R S D  D+AE  G  + +  PL
Sbjct:  423 RGTNFEYIPFGSGRRICPGIGFAMALVEVTLANLVNRFNWRMDARLSGDEYDLAEATGIDVCRKFPL 489

HSP 2 Score: 28.1054 bits (61), Expect = 6.134e-9
Identity = 12/23 (52.17%), Postives = 14/23 (60.87%), Query Frame = -3 
Query:  263 NAWAIHRGS*VMGVKATTFKPER 331
            NAWAI R     G+ A  F+PER
Sbjct:  392 NAWAIQRDIVTWGIDAEEFRPER 414
[Close]
BLAST of HO062872 vs. SwissProt
Match: C82A3_SOYBN (Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 7.042e-9
Identity = 39/117 (33.33%), Postives = 58/117 (49.57%), Query Frame = -1 
Query:   28 GTTRHHSIRSMHGPFTEDPKSW----E*RPQP--SSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVN 360
            GT   H++  +H     DP  W    E +P+   ++ +    +G   +++ FG GRRVC G +L L  +  TLA L+  FD    S + +DM E  GF  TK  PL+ + K R   N
Sbjct:  410 GTRLIHNLWKIH----RDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKPRQSPN 522
[Close]
BLAST of HO062872 vs. SwissProt
Match: C71A8_MENPI (Cytochrome P450 71A8 OS=Mentha piperita GN=CYP71A8 PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 7.042e-9
Identity = 35/91 (38.46%), Postives = 50/91 (54.95%), Query Frame = -1 
Query:   52 DPKSWE*----RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSD-DMIDMAERDGFVLTKLIPLKAM 309
            DP SW+     RP+         KG   ++I FG GRR CPG    + T+  TLA L+Q FDW+   +   +DM+ER G  + ++IPL A+
Sbjct:  408 DPTSWDEPEKFRPERFLNSSVDFKGLDFELIPFGAGRRGCPGTTFPMATLEFTLANLMQKFDWELPHECRELDMSERPGVAIRRVIPLLAI 498
[Close]
BLAST of HO062872 vs. TrEMBL
Match: Q9XFX0_CICAR (Cytochrome P450 monooxygenase OS=Cicer arietinum GN=cyp81E3v2 PE=2 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 2.052e-38
Identity = 81/98 (82.65%), Postives = 86/98 (87.76%), Query Frame = -1 
Query:   16 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 309
            DPKSW         R + KKGE++KVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKL+PLKAMCKTRPVVN+I K
Sbjct:  402 DPKSWSEATTFKPER-FDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLVPLKAMCKTRPVVNKIFK 498
[Close]
BLAST of HO062872 vs. TrEMBL
Match: Q9ZRW6_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.019e-37
Identity = 79/98 (80.61%), Postives = 85/98 (86.73%), Query Frame = -1 
Query:   16 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 309
            DPKSW         R + KKGE++KVIAFGMGRRVCPGEALALRTISMTLALL+QCFDWKRTSDD IDMAERDGFVLTKLIPLKAMCKTRPVVN++ K
Sbjct:  402 DPKSWSEATSFKPER-FDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLVQCFDWKRTSDDKIDMAERDGFVLTKLIPLKAMCKTRPVVNKVFK 498
[Close]
BLAST of HO062872 vs. TrEMBL
Match: Q6WNR0_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 2.870e-32
Identity = 66/98 (67.35%), Postives = 80/98 (81.63%), Query Frame = -1 
Query:   16 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 309
            DP++W         R + KKGE++K+IAFGMGRR CPGE LALR ISMTLALL+QCFDWKR +D+ IDM+ERDGF +TKL+PLKAMCKTRPVVN++ K
Sbjct:  402 DPETWSEATTFKPER-FDKKGELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTKLLPLKAMCKTRPVVNKVFK 498
[Close]
BLAST of HO062872 vs. TrEMBL
Match: Q9ZWF2_GLYEC (Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-31 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 2.430e-31
Identity = 66/98 (67.35%), Postives = 78/98 (79.59%), Query Frame = -1 
Query:   16 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 309
            DP+ W         R + KKGE++K+IAFGMGRR CPGE LA+R ISMTLALLIQCFDWK T+ D IDMAERDGF LTKL+PLKAMCK+RPV+N++ K
Sbjct:  402 DPELWTEATTFKPER-FEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLTNGDKIDMAERDGFTLTKLVPLKAMCKSRPVINKVFK 498
[Close]
BLAST of HO062872 vs. TrEMBL
Match: Q9MBE4_LOTJA (Cytochrome P450 OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 2.970e-29
Identity = 63/98 (64.29%), Postives = 75/98 (76.53%), Query Frame = -1 
Query:   16 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 309
            DP+ W         R + KKGE++K+I FG+GRR CPGE LA+R ISMTLALLIQCFDWKR SD+ IDM ERDGFVL K IP+KAMCK+RPV+N + K
Sbjct:  403 DPQLWTEATTFKPER-FDKKGELEKLIPFGLGRRACPGELLAIRAISMTLALLIQCFDWKRVSDEEIDMGERDGFVLMKSIPVKAMCKSRPVINNVFK 499
[Close]
BLAST of HO062872 vs. TrEMBL
Match: Q8L6V5_CICAR (Putative cytochrome P450 monooxygenase (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 6.193e-27
Identity = 61/61 (100.00%), Postives = 61/61 (100.00%), Query Frame = -1 
Query:   16 GEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 198
            GEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK
Sbjct:    3 GEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 63
[Close]
BLAST of HO062872 vs. TrEMBL
Match: Q2LAL0_SOYBN (Cytochrome P450 monooxygenase CYP81E11 OS=Glycine max GN=CYP81E11 PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.062e-22
Identity = 48/96 (50.00%), Postives = 70/96 (72.92%), Query Frame = -1 
Query:   22 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 309
            DPK W+        R + ++GE KK++AFGMGRR CPGE +A++++S TL L+IQCFDWKR S+  +DM E +   L++LIPL+AMCK RP+ +++
Sbjct:  406 DPKIWKDATSFKPER-FDEEGEEKKLVAFGMGRRACPGEPMAMQSVSYTLGLMIQCFDWKRVSEKKLDMTENNWITLSRLIPLEAMCKARPLASKV 500
[Close]
BLAST of HO062872 vs. TrEMBL
Match: Q2LAK9_SOYBN (Cytochrome P450 monooxygenase CYP81E10 OS=Glycine max GN=CYP82E13 PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 7.835e-22
Identity = 45/77 (58.44%), Postives = 62/77 (80.52%), Query Frame = -1 
Query:   22 KGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 252
            +GE KK++AFGMGRR CPGE +A++++S TL LLIQCFDWKR S++ +DM E +   L++LIPL+AMCK RP+  +I
Sbjct:  424 EGEEKKLVAFGMGRRACPGEPMAMQSVSFTLGLLIQCFDWKRVSEEKLDMTENNWITLSRLIPLEAMCKARPLATKI 500
[Close]
BLAST of HO062872 vs. TrEMBL
Match: Q0WTJ5_ARATH (Cytochrome P450 like protein (Fragment) OS=Arabidopsis thaliana GN=At4g37330 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.930e-20
Identity = 47/97 (48.45%), Postives = 65/97 (67.01%), Query Frame = -1 
Query:   19 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 309
            DP+ WE  P+      + K+GE +K+I+FGMGRR CPG  LA R I+  L  L+QCF+W+R  +D +DM E  G  L K IPL+AMCK R +V+++I
Sbjct:  384 DPEVWE-DPEIFKPERFEKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKAIPLRAMCKARSIVDKLI 479
[Close]
BLAST of HO062872 vs. TrEMBL
Match: O23155_ARATH (At4g37330 OS=Arabidopsis thaliana GN=AT4g37330 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.930e-20
Identity = 47/97 (48.45%), Postives = 65/97 (67.01%), Query Frame = -1 
Query:   19 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 309
            DP+ WE  P+      + K+GE +K+I+FGMGRR CPG  LA R I+  L  L+QCF+W+R  +D +DM E  G  L K IPL+AMCK R +V+++I
Sbjct:  397 DPEVWE-DPEIFKPERFEKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKAIPLRAMCKARSIVDKLI 492
[Close]
BLAST of HO062872 vs. TAIR peptide
Match: AT4G37330.1 (| Symbols: CYP81D4 | cytochrome P450, family 81, subfamily D, polypeptide 4 | chr4:17562547-17564569 REVERSE LENGTH=492)

HSP 1 Score: 102.064 bits (253), Expect = 9.980e-23
Identity = 47/97 (48.45%), Postives = 65/97 (67.01%), Query Frame = -1 
Query:   19 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 309
            DP+ WE  P+      + K+GE +K+I+FGMGRR CPG  LA R I+  L  L+QCF+W+R  +D +DM E  G  L K IPL+AMCK R +V+++I
Sbjct:  397 DPEVWE-DPEIFKPERFEKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKAIPLRAMCKARSIVDKLI 492
[Close]
BLAST of HO062872 vs. TAIR peptide
Match: AT4G37340.1 (| Symbols: CYP81D3 | cytochrome P450, family 81, subfamily D, polypeptide 3 | chr4:17564953-17566706 REVERSE LENGTH=500)

HSP 1 Score: 97.0561 bits (240), Expect = 3.210e-21
Identity = 46/98 (46.94%), Postives = 64/98 (65.31%), Query Frame = -1 
Query:   19 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAE-RDGFVLTKLIPLKAMCKTRPVVNRII 309
            DPK W+  P       + K+GE +K++ FG+GRR CPG  LA R  S+T+  LIQCF+W+R  ++ +DM E   G ++ K IPL AMCK RPVV +I+
Sbjct:  400 DPKIWD-DPTSFKPERFEKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLVAMCKARPVVGKIL 496
[Close]
BLAST of HO062872 vs. TAIR peptide
Match: AT3G28740.1 (| Symbols: CYP81D1 | Cytochrome P450 superfamily protein | chr3:10788764-10790552 REVERSE LENGTH=509)

HSP 1 Score: 93.9745 bits (232), Expect = 2.718e-20
Identity = 43/98 (43.88%), Postives = 64/98 (65.31%), Query Frame = -1 
Query:   19 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERD-GFVLTKLIPLKAMCKTRPVVNRII 309
            DPK WE  P+      + KKGE KK++ FG+GRR CPG  LA R +++ L  L+QCF+W+R  +  +DM E + G  + K   L+AMCK RP+V++++
Sbjct:  408 DPKLWE-EPEKFKPERFEKKGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAMCKARPIVHKVL 504
[Close]
BLAST of HO062872 vs. TAIR peptide
Match: AT4G37410.1 (| Symbols: CYP81F4 | cytochrome P450, family 81, subfamily F, polypeptide 4 | chr4:17590848-17592780 FORWARD LENGTH=501)

HSP 1 Score: 92.8189 bits (229), Expect = 6.055e-20
Identity = 41/99 (41.41%), Postives = 66/99 (66.67%), Query Frame = -1 
Query:   22 DPKSWE*RPQPSSRR---GWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 309
            DP  W    + +  R   G  +K +++ +IAFG GRR+CPG  LA + +++ L  LIQCFDWK+ ++  IDM+E  G  +  ++PL+A+CKTRP++N++
Sbjct:  397 DPDLWTEPERFNPERFNGGEGEKDDVRMLIAFGSGRRICPGVGLAHKIVTLALGSLIQCFDWKKVNEKEIDMSEGPGMAMRMMVPLRALCKTRPIMNKL 495
[Close]
BLAST of HO062872 vs. TAIR peptide
Match: AT4G37320.1 (| Symbols: CYP81D5 | cytochrome P450, family 81, subfamily D, polypeptide 5 | chr4:17559742-17561690 REVERSE LENGTH=495)

HSP 1 Score: 92.8189 bits (229), Expect = 6.055e-20
Identity = 42/97 (43.30%), Postives = 60/97 (61.86%), Query Frame = -1 
Query:   19 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 309
            DP  WE  P+      + K+GE +K++ FGMGRR CPG  L  R +S+ L  LIQ F+W+R   +++DM E +G  + K  PL+AMCK R +V + I
Sbjct:  400 DPGLWE-EPERFKPERFEKEGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPKATPLRAMCKARAIVGKTI 495
[Close]
BLAST of HO062872 vs. TAIR peptide
Match: AT5G36220.1 (| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1 | chr5:14253827-14256015 REVERSE LENGTH=502)

HSP 1 Score: 92.4337 bits (228), Expect = 7.908e-20
Identity = 43/97 (44.33%), Postives = 62/97 (63.92%), Query Frame = -1 
Query:   19 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 309
            DP +W+  P       + K+ E +K++AFG+GRR CPG  LA R + + L  LIQCF+W+R  +  +DM E  G  + K IPLKA+CK RP +++II
Sbjct:  406 DPNTWD-DPDSFKPERFEKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKII 501
[Close]
BLAST of HO062872 vs. TAIR peptide
Match: AT1G66540.2 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24825446-24826502 FORWARD LENGTH=327)

HSP 1 Score: 91.6633 bits (226), Expect = 1.349e-19
Identity = 41/96 (42.71%), Postives = 60/96 (62.50%), Query Frame = -1 
Query:   22 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 309
            DP+ W+  P       + K+GE  K++ FG+GRR CPG  LA R +S++L  LIQCF+W+R  ++ +DM E  G  + + IPL AMC+ R  V +I
Sbjct:  228 DPRLWD-DPASFKPERFEKEGETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRARAFVGKI 322
[Close]
BLAST of HO062872 vs. TAIR peptide
Match: AT1G66540.1 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24824837-24826502 FORWARD LENGTH=386)

HSP 1 Score: 91.6633 bits (226), Expect = 1.349e-19
Identity = 41/96 (42.71%), Postives = 60/96 (62.50%), Query Frame = -1 
Query:   22 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 309
            DP+ W+  P       + K+GE  K++ FG+GRR CPG  LA R +S++L  LIQCF+W+R  ++ +DM E  G  + + IPL AMC+ R  V +I
Sbjct:  287 DPRLWD-DPASFKPERFEKEGETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRARAFVGKI 381
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BLAST of HO062872 vs. TAIR peptide
Match: AT4G37360.1 (| Symbols: CYP81D2 | cytochrome P450, family 81, subfamily D, polypeptide 2 | chr4:17567124-17568858 REVERSE LENGTH=499)

HSP 1 Score: 90.8929 bits (224), Expect = 2.301e-19
Identity = 41/97 (42.27%), Postives = 61/97 (62.89%), Query Frame = -1 
Query:   19 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 309
            DP  W+  P       + K+GE KK++ FG+GRR CPG  LA R ++++L  LIQCF+W+R  ++ +DM E  G  + K  PL+AMC+ R  V +I+
Sbjct:  400 DPLLWD-DPTSFKPERFEKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCRARDFVGKIL 495
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BLAST of HO062872 vs. TAIR peptide
Match: AT4G37370.1 (| Symbols: CYP81D8 | cytochrome P450, family 81, subfamily D, polypeptide 8 | chr4:17569971-17571678 REVERSE LENGTH=497)

HSP 1 Score: 85.8853 bits (211), Expect = 7.401e-18
Identity = 41/96 (42.71%), Postives = 62/96 (64.58%), Query Frame = -1 
Query:   22 DPKSWE*RPQPSSRRGWTKKGEMKKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 309
            DP+ W+  P       + K+GE +K++ FG+GRR CPG  LA R I++TL  LIQC +W++  ++ +DM+E  G  + K  PL+AMC+ RP V +I
Sbjct:  399 DPQLWD-DPMSFKPERFEKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLEAMCRARPSVVKI 492
The following BLAST results are available for this feature:
BLAST of HO062872 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
C81E1_GLYEC1.319e-3165.31Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata ... [more]
C81D1_ARATH7.506e-1944.33Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CY... [more]
C81F1_ARATH2.499e-1436.00Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CY... [more]
C93A2_SOYBN2.189e-1036.27Cytochrome P450 93A2 OS=Glycine max GN=CYP93A2 PE=... [more]
C93A1_SOYBN2.859e-1036.27Cytochrome P450 93A1 OS=Glycine max GN=CYP93A1 PE=... [more]
C93A3_SOYBN6.369e-1037.36Cytochrome P450 93A3 OS=Glycine max GN=CYP93A3 PE=... [more]
C71AE_ARATH3.648e-937.31Cytochrome P450 71A14 OS=Arabidopsis thaliana GN=C... [more]
C71AF_ARATH6.134e-938.81Cytochrome P450 71A15 OS=Arabidopsis thaliana GN=C... [more]
C82A3_SOYBN7.042e-933.33Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=... [more]
C71A8_MENPI7.042e-938.46Cytochrome P450 71A8 OS=Mentha piperita GN=CYP71A8... [more]
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BLAST of HO062872 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9XFX0_CICAR2.052e-3882.65Cytochrome P450 monooxygenase OS=Cicer arietinum G... [more]
Q9ZRW6_CICAR1.019e-3780.61Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2... [more]
Q6WNR0_MEDTR2.870e-3267.35Isoflavone 2'-hydroxylase OS=Medicago truncatula P... [more]
Q9ZWF2_GLYEC2.430e-3167.35Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-... [more]
Q9MBE4_LOTJA2.970e-2964.29Cytochrome P450 OS=Lotus japonicus PE=2 SV=1[more]
Q8L6V5_CICAR6.193e-27100.00Putative cytochrome P450 monooxygenase (Fragment) ... [more]
Q2LAL0_SOYBN2.062e-2250.00Cytochrome P450 monooxygenase CYP81E11 OS=Glycine ... [more]
Q2LAK9_SOYBN7.835e-2258.44Cytochrome P450 monooxygenase CYP81E10 OS=Glycine ... [more]
Q0WTJ5_ARATH1.930e-2048.45Cytochrome P450 like protein (Fragment) OS=Arabido... [more]
O23155_ARATH1.930e-2048.45At4g37330 OS=Arabidopsis thaliana GN=AT4g37330 PE=... [more]
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BLAST of HO062872 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10
Match NameE-valueIdentityDescription
AT4G37330.19.980e-2348.45| Symbols: CYP81D4 | cytochrome P450, family 81, s... [more]
AT4G37340.13.210e-2146.94| Symbols: CYP81D3 | cytochrome P450, family 81, s... [more]
AT3G28740.12.718e-2043.88| Symbols: CYP81D1 | Cytochrome P450 superfamily p... [more]
AT4G37410.16.055e-2041.41| Symbols: CYP81F4 | cytochrome P450, family 81, s... [more]
AT4G37320.16.055e-2043.30| Symbols: CYP81D5 | cytochrome P450, family 81, s... [more]
AT5G36220.17.908e-2044.33| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1... [more]
AT1G66540.21.349e-1942.71| Symbols: | Cytochrome P450 superfamily protein ... [more]
AT1G66540.11.349e-1942.71| Symbols: | Cytochrome P450 superfamily protein ... [more]
AT4G37360.12.301e-1942.27| Symbols: CYP81D2 | cytochrome P450, family 81, s... [more]
AT4G37370.17.401e-1842.71| Symbols: CYP81D8 | cytochrome P450, family 81, s... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450GENE3D1.10.630.10no descriptioncoord: 13..83
score: 1.3
IPR001128Cytochrome P450PANTHERPTHR19383CYTOCHROME P450coord: 12..50
score: 3.9
IPR001128Cytochrome P450PFAMPF00067p450coord: 14..72
score: 5.1
IPR001128Cytochrome P450SUPERFAMILY48264Cytochrome P450coord: 7..83
score: 6.7
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 19..29
score: 4.1e-06coord: 29..52
score: 4.1
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 22..31
score
NoneNo IPR availablePANTHERPTHR19383:SF42CYTOCHROME P450 SUBFAMILY 81Dcoord: 12..50
score: 3.9

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO062872 ID=HO062872; Name=HO062872; organism=Cicer arietinum; type=EST; length=393bp
CGTGAGAATTAATTACTTGATAATTCTGTTTACGACCGGACGAGTTTTAC
ACATGGCCTTTAATGGAATTAATTTTGTCAAAACAAATCCATCTCGTTCT
GCCATATCAATCATGTCATCGCTTGTACGTTTCCAATCAAAGCATTGAAT
CAATAATGCCAAAGTCATGCTAATTGTTCGAAGAGCCAAAGCTTCTCCAG
GACACACTCTTCTTCCCATCCCAAATGCAATCACCTTTTTCATCTCCCCC
TTTTTTGTCCAACCTCTCCGGCTTGAAGGTTGTGGCCTTCACTCCCATGA
CTTAGGATCCTCTGTGAATGGCCCATGCATTGATCTAATACTATGGTGTC
TCGTGGTACCTGCCCGGGCGGCCGCTCTGAAATCTGAATTTCT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004497monooxygenase activity
GO:0005506iron ion binding
GO:0009055electron transfer activity
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114obsolete oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR002401Cyt_P450_E_grp-I
IPR017972Cyt_P450_CS