HO066780

EST Overview


Name	HO066780
Unique Name	HO066780
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	550

Libraries


Library Name	Type
LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of HO066780 vs. SwissProt
Match: NAS_SOLLC (Nicotianamine synthase OS=Solanum lycopersicum GN=CHLN PE=2 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.166e-34
Identity = 80/148 (54.05%), Postives = 103/148 (69.59%), Query Frame = -1

Query:  104 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKCSVNQEVYL----------KSKCCEVEGFINPLNHGNVIEELSXDE 517
            D+S RM F   ++  V   LKDY+VVFLAALVGMDK++K KV++HLAK M+ GA L++RSA+GARAFLYP++D   DL+GFEVLS++HP DEVINSVI+ARK  V     L          K  C E+  F NPLN  N++EE + +E
Sbjct:  172 DMSSRMTFHTADVMDVTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDP-RDLRGFEVLSVYHPTDEVINSVIIARKLPVPSVPLLDGLGAYVLPSKCACAEIHAF-NPLNKMNLVEEFALEE 317

[Close]

BLAST of HO066780 vs. SwissProt
Match: NAS2_ARATH (Nicotianamine synthase 2 OS=Arabidopsis thaliana GN=NAS2 PE=2 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 1.861e-32
Identity = 78/153 (50.98%), Postives = 99/153 (64.71%), Query Frame = -1

Query:  104 RGQDLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARK---------CSVNQEVYLKSKCCEVEGFINPLNHG---NVIEELSXDE 526
            R  DLSKRM F   ++   K  L  Y+VVFLAALVGMDK+ K K I HL K MA GA++++RSA+G RAFLYPIVD  CDLKGFEVL+I+HP D+V+NSV++ARK           + + V +   C +V   +N  N G   N+IEE S  E
Sbjct:  171 RDSDLSKRMIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVD-SCDLKGFEVLTIYHPSDDVVNSVVIARKLGGSNGARGSQIGRCVVMPCNCSKVHAILN--NRGMEKNLIEEYSAIE 320

[Close]

BLAST of HO066780 vs. SwissProt
Match: NAS4_ARATH (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana GN=NAS4 PE=2 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 3.510e-31
Identity = 73/145 (50.34%), Postives = 99/145 (68.28%), Query Frame = -1

Query:  113 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKCS----------VNQEVYLKSKCCEVEGFINPLNHGNVIEELS 517
            DLS+RMFF   +I  V   LK ++VVFLAALVGMDKKEK KV+ HL K M+ GA+L++RSA+G RAFLYPIV+  CDL+GFEVLS++HP DEVINS++++RK            ++Q   L   C ++   +N     ++IEE +
Sbjct:  175 DLSQRMFFHTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEP-CDLEGFEVLSVYHPTDEVINSIVISRKLGEDANGVVHDHIDQASDLACNCSKIHVIMN--KKKSIIEEFA 316

[Close]

BLAST of HO066780 vs. SwissProt
Match: NAS1_ARATH (Nicotianamine synthase 1 OS=Arabidopsis thaliana GN=NAS1 PE=2 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 5.069e-30
Identity = 72/150 (48.00%), Postives = 95/150 (63.33%), Query Frame = -1

Query:  104 RGQDLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKC------SVNQE---VYLKSKCCEVEGFINPLNHGNVIEELSXDE 526
            R  DLSKRM F   ++      L  Y+VVFLAALVGMDK+ K K I HL K MA GA+L++RSA+  RAFLYPIVD   DLKGF++L+I+HP D+V+NSV++ARK        VN     +++   C ++   +N     N+IEE S  E
Sbjct:  172 RDPDLSKRMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD-SSDLKGFQLLTIYHPTDDVVNSVVIARKLGGPTTPGVNGTRGCMFMPCNCSKIHAIMNNRGKKNMIEEFSAIE 320

[Close]

BLAST of HO066780 vs. SwissProt
Match: NAS3_ARATH (Nicotianamine synthase 3 OS=Arabidopsis thaliana GN=NAS3 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 6.620e-30
Identity = 65/102 (63.73%), Postives = 84/102 (82.35%), Query Frame = -1

Query:  212 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARK 517
            D+S+RMFF   +I  V   LK ++VVFLAALVGM+K+EK KVI HL K MA GA+L++RSA+G RAFLYPIV+  CDL+GFEVLSI+HP D+VINSV++++K
Sbjct:  174 DISQRMFFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVEP-CDLQGFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of HO066780 vs. SwissProt
Match: NAS3_ORYSJ (Nicotianamine synthase 3 OS=Oryza sativa subsp. japonica GN=NAS3 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.209e-23
Identity = 59/104 (56.73%), Postives = 75/104 (72.12%), Query Frame = -1

Query:  212 QDLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVD-HCCDLKGFEVLSIFHPIDEVINSVIVARK 520
            +DLS RM F  +++ +V   L  Y+VVFLAALVGM  +EK +++ HL K MA GA LVVRSA+GAR FLYP+VD       GF+VL++ HP  EVINSVI+ARK
Sbjct:  189 RDLSARMAFHTSDVAHVTTDLAAYDVVFLAALVGMAAEEKARMVEHLGKHMAPGAALVVRSAHGARGFLYPVVDPEEIRRGGFDVLAVHHPEGEVINSVIIARK 292

[Close]

BLAST of HO066780 vs. SwissProt
Match: NAS3_ORYSI (Nicotianamine synthase 3 OS=Oryza sativa subsp. indica GN=NAS3 PE=2 SV=2)

HSP 1 Score: 109.383 bits (272), Expect = 1.209e-23
Identity = 59/104 (56.73%), Postives = 75/104 (72.12%), Query Frame = -1

Query:  212 QDLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVD-HCCDLKGFEVLSIFHPIDEVINSVIVARK 520
            +DLS RM F  +++ +V   L  Y+VVFLAALVGM  +EK +++ HL K MA GA LVVRSA+GAR FLYP+VD       GF+VL++ HP  EVINSVI+ARK
Sbjct:  189 RDLSARMAFHTSDVAHVTTDLAAYDVVFLAALVGMAAEEKARMVEHLGKHMAPGAALVVRSAHGARGFLYPVVDPEEIRRGGFDVLAVHHPEGEVINSVIIARK 292

[Close]

BLAST of HO066780 vs. SwissProt
Match: NAS2_ORYSJ (Nicotianamine synthase 2 OS=Oryza sativa subsp. japonica GN=NAS2 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.209e-23
Identity = 72/145 (49.66%), Postives = 87/145 (60.00%), Query Frame = -1

Query:  161 ERPPGQVRGQD--LSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLK--GFEVLSIFHPIDEVINSVIVARKCSVNQ------------EVYLKSKCCEVE 547
            ER     RG D  L  RM F   ++  +   L  Y+VVFLAALVGM  +EK  VI HL   MADGA LVVRSA+GAR FLYPIVD   D++  GF+VL+++HP DEVINSVIVARK    +             V    KCC++E
Sbjct:  164 ERARRLFRGADEGLGARMAFHTADVATLTGELGAYDVVFLAALVGMAAEEKAGVIAHLGAHMADGAALVVRSAHGARGFLYPIVD-LEDIRRGGFDVLAVYHPDDEVINSVIVARKADPRRGGGLAGARGAVPVVSPPCKCCKME 307

[Close]

BLAST of HO066780 vs. SwissProt
Match: NAS9_HORVU (Nicotianamine synthase 9 OS=Hordeum vulgare GN=NAS9 PE=1 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.595e-23
Identity = 62/116 (53.45%), Postives = 77/116 (66.38%), Query Frame = -1

Query:  206 ERPPGQVRGQ-DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVD-HCCDLKGFEVLSIFHPIDEVINSVIVARKCS 547
            ER    VR   D   RM F   ++  V   L+ Y+VVFLAALVGM  +EK +++ HL + MA GA LVVRSA+GAR FLYP+VD       GFEVL++ HP DEVINSVI+ARK +
Sbjct:  171 ERASRLVRADADAGARMAFRTADVADVTTELEGYDVVFLAALVGMAAEEKARLVEHLGRHMAPGAALVVRSAHGARGFLYPVVDPEEIRRGGFEVLTVHHPEDEVINSVIIARKAA 286

[Close]

BLAST of HO066780 vs. SwissProt
Match: NAS1_ORYSJ (Nicotianamine synthase 1 OS=Oryza sativa subsp. japonica GN=NAS1 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.024e-22
Identity = 66/115 (57.39%), Postives = 75/115 (65.22%), Query Frame = -1

Query:  212 ERPPGQVRGQD--LSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVD-HCCDLKGFEVLSIFHPIDEVINSVIVARK 547
            ER     RG D  L  RM F   ++  +   L  Y+VVFLAALVGM  +EK  VI HL   MADGA LVVRSA+GAR FLYPIVD       GF+VL++ HP DEVINSVIVARK
Sbjct:  164 ERARRLFRGADEGLGARMAFHTADVATLTGELGAYDVVFLAALVGMAAEEKAGVIAHLGAHMADGAALVVRSAHGARGFLYPIVDPEDVRRGGFDVLAVCHPEDEVINSVIVARK 278

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BLAST of HO066780 vs. TrEMBL
Match: C6TI45_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 3.376e-44
Identity = 99/140 (70.71%), Postives = 111/140 (79.29%), Query Frame = -1

Query:   98 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKCSVNQEVYLKSKCCEVEGFINPLNHGNVIEELSXDEKA 517
            DLSKRMFF   +I  V N LKDYNVVFLAALVGMD KEK +VI+HLAK MA GAIL++RSA+GARAFLYP+VD   DLKGFE LS+FHP DEVINSVIVARK   +  V L SKC  VEGF N  NHGNVIEEL+ D++A
Sbjct:  172 DLSKRMFFHTCDILNVSNGLKDYNVVFLAALVGMDHKEKGRVISHLAKFMAPGAILLLRSAHGARAFLYPVVDPSSDLKGFEALSVFHPTDEVINSVIVARKGLYSSPV-LASKCSGVEGF-NHFNHGNVIEELTVDDQA 309

[Close]

BLAST of HO066780 vs. TrEMBL
Match: B9A9Z2_LOTJA (Nicotianamine synthase OS=Lotus japonicus GN=LjNAS2 PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 3.376e-44
Identity = 100/137 (72.99%), Postives = 111/137 (81.02%), Query Frame = -1

Query:  113 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKCSVNQEVYLKSKCCEV--EGFINPLNHGNVIEELS 517
            DLSKRMFF   +I +V N LK+YNVVFLAALVGMDKK K  VINHLAK MA GA+LV+RSA+GARAFLYP+VD   DLKGFEVLS+FHP DEVINSVIVARK SVN  + L SKC E+  EGF N LNH NVI+ELS
Sbjct:  171 DLSKRMFFHTTDIAHVSNALKEYNVVFLAALVGMDKKGKESVINHLAKHMAPGAVLVLRSAHGARAFLYPVVDP-SDLKGFEVLSVFHPTDEVINSVIVARKHSVNPGILLSSKCHELAAEGF-NMLNHRNVIDELS 305

[Close]

BLAST of HO066780 vs. TrEMBL
Match: B9RFJ1_RICCO (Nicotianamine synthase, putative OS=Ricinus communis GN=RCOM_1435160 PE=4 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 3.160e-42
Identity = 92/150 (61.33%), Postives = 121/150 (80.67%), Query Frame = -1

Query:  101 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARK-----CSVNQEVY------LKSKCCEVEGFINPLNHGNVIEELSXDEK 517
            DLSKRMFF   +I  V+N L++++VVFLAALVGMDK+EK +VI+HLAK MA GAIL++RSA+GARAFLYP+VD  CDL+GFEVLS+FHP DEVINSV++A+K      S++Q+V+      L SKC E++ F NPLNHGN+IEEL+ +++
Sbjct:  171 DLSKRMFFHTTDIMNVENGLQEFDVVFLAALVGMDKEEKIRVIDHLAKYMAPGAILMLRSAHGARAFLYPVVDP-CDLRGFEVLSVFHPTDEVINSVVIAQKHQLPIQSIDQQVFVPNNIILPSKCSEIQAF-NPLNHGNMIEELAIEDQ 318

[Close]

BLAST of HO066780 vs. TrEMBL
Match: A3DUI9_MALXI (Nicotianamine synthase OS=Malus xiaojinensis PE=2 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.734e-40
Identity = 88/154 (57.14%), Postives = 112/154 (72.73%), Query Frame = -1

Query:  101 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKCSVNQE--------------VYLKSKCCEVEGFINPLN-HGNVIEELSXDEK 517
            DLSKRM F   +I  V   LKDY +VFLAALVGMDK EK K+I+HLAK MA GA L++RSA+GARAFLYP++D C DL+GFEVLS+FHP DEVINS+++ARK ++  +              + L SKCCE+E F NPL+ HGN+IEE + +E+
Sbjct:  171 DLSKRMVFHTTDIMDVTTALKDYEIVFLAALVGMDKMEKLKIIDHLAKYMAPGATLMLRSAHGARAFLYPVIDPCDDLRGFEVLSVFHPTDEVINSIVIARKYALPVQHSSPIDSQGLNAGAIILPSKCCELEAF-NPLHKHGNMIEEFAFEEQ 323

[Close]

BLAST of HO066780 vs. TrEMBL
Match: C6TI94_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.468e-39
Identity = 86/151 (56.95%), Postives = 115/151 (76.16%), Query Frame = -1

Query:  101 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKC-------SVNQE-----VYLKSKCCEVEGFINPLNHGNVIEELSXDEK 517
            DLSKRMFF  N+I  V   LK+Y VV+LAALVGMDK+ K ++I+HL K M  GA+L++RSA+GARAFLYP+V+  CDL+GFEVLS+FHP DEV+NSV++ARK        S++Q+     + L +KC E++ F NPLNHGN+IEEL+ +E+
Sbjct:  171 DLSKRMFFHTNDILEVSTALKEYEVVYLAALVGMDKEGKNRIIDHLGKHMNPGALLMLRSAHGARAFLYPVVEP-CDLRGFEVLSVFHPSDEVVNSVVIARKLYHPVPMHSLDQQALVGSMILPNKCSEIQAF-NPLNHGNMIEELAVEEQ 319

[Close]

BLAST of HO066780 vs. TrEMBL
Match: B9N4R2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_581960 PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 4.271e-39
Identity = 87/151 (57.62%), Postives = 116/151 (76.82%), Query Frame = -1

Query:  101 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKC-----SVNQE-------VYLKSKCCEVEGFINPLNHGNVIEELSXDEK 517
            DLS RMFF   +I  V + L++++VVFLAALVGMDK+EK KVI+HLAK MA GAIL++RSA+GARAFLYP++D  CDL+GFEVLS+FHP DEVINSV++ARK      S++Q+       + L +KCCE+  F NPL+H  ++EEL+ +E+
Sbjct:  171 DLSSRMFFHTTDIMDVTSELQEFDVVFLAALVGMDKEEKVKVIDHLAKQMAPGAILMLRSAHGARAFLYPVIDP-CDLRGFEVLSVFHPSDEVINSVVIARKYSLPINSIDQQQTGLGSMIVLPNKCCEILAF-NPLSHVTMVEELAVEEQ 319

[Close]

BLAST of HO066780 vs. TrEMBL
Match: A2Q2X7_MEDTR (Nicotianamine synthase OS=Medicago truncatula GN=MtrDRAFT_AC152185g32v2 PE=4 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 1.623e-38
Identity = 84/111 (75.68%), Postives = 94/111 (84.68%), Query Frame = -1

Query:  194 RGQDLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKCSVNQE 526
            +  DLSKRM F  ++I  VKN LK++NVVFLAALVGMDKKEK KVINHLAK MA GAILV+RSA+GA+AFLY +VD  CDLKGFEVLSIFHP DEVINSVIVARK  VNQ+
Sbjct:  169 KDNDLSKRMLFHTSDIVDVKNELKEFNVVFLAALVGMDKKEKAKVINHLAKYMAPGAILVLRSAHGAKAFLYHVVDPSCDLKGFEVLSIFHPTDEVINSVIVARKGLVNQQ 279

[Close]

BLAST of HO066780 vs. TrEMBL
Match: B9RZP0_RICCO (Nicotianamine synthase, putative OS=Ricinus communis GN=RCOM_1000280 PE=4 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 6.818e-37
Identity = 87/147 (59.18%), Postives = 110/147 (74.83%), Query Frame = -1

Query:  101 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKC-----SVNQEV---YLKSKCCEVEGFINPLNHGNVIEELSXDEK 517
            +LS+RMFF   NI  V + L +Y VV LAALVGMDKKEK +VI HLA+ MA GAIL++RSA+GARAFLYP++D   DL+GFEVLS+FHP DEVINSVI+ARK      S+N  +    L SKC +++GF  P+ H N+IEEL+ DE+
Sbjct:  171 NLSERMFFHTANIMSVSSSLTEYEVVILAALVGMDKKEKVQVIKHLAEHMAPGAILLLRSAHGARAFLYPVIDP-QDLEGFEVLSVFHPTDEVINSVIIARKFLRAVHSLNTGIGSALLPSKCSDIQGF-KPITHTNLIEELTVDEQ 315

[Close]

BLAST of HO066780 vs. TrEMBL
Match: A5B0Y3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_020491 PE=4 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 8.335e-35
Identity = 84/146 (57.53%), Postives = 108/146 (73.97%), Query Frame = -1

Query:  104 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKC-----SVNQ---EVYLKSKCCEVEGFINPLNHGNVIEELSXDE 517
            D SKRM F   +I  V N LK+Y+VV+LAALVGMDK+EK +V++HLAK MA GA+L++RSA+GARAFLYP++D  CDL+GFEVLSIFHP DEVINSV+VARK      S++Q    + L SKC E++ F       N +EEL+ +E
Sbjct:  171 DFSKRMLFHTTDIMNVTNELKEYDVVYLAALVGMDKEEKIQVMDHLAKYMAPGALLMLRSAHGARAFLYPVIDP-CDLRGFEVLSIFHPTDEVINSVVVARKFPMHVHSLDQGLGPMILPSKCSEIQVF-------NPLEELTIEE 308

[Close]

BLAST of HO066780 vs. TrEMBL
Match: D7UA30_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_60.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031240001 PE=4 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 1.422e-34
Identity = 83/148 (56.08%), Postives = 109/148 (73.65%), Query Frame = -1

Query:   98 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKC-----SVNQ---EVYLKSKCCEVEGFINPLNHGNVIEELSXDEKA 517
            D SKRM F   +I  V N LK+Y+VV+LAALVGMDK+EK +V++HLAK MA GA+L++RSA+GARAFLYP++D  CDL+GFEVLSIFHP DEVINSV+VARK      S++Q    + L SKC E++ F       N +EEL+ ++ +
Sbjct:  319 DFSKRMLFHTTDIMNVTNELKEYDVVYLAALVGMDKEEKIQVMDHLAKHMAPGALLMLRSAHGARAFLYPVIDP-CDLRGFEVLSIFHPTDEVINSVVVARKFPMHVHSLDQGLGPMILPSKCSEIQVF-------NPLEELTIEDSS 458

[Close]

BLAST of HO066780 vs. TAIR peptide
Match: AT5G56080.1 (| Symbols: ATNAS2, NAS2 | nicotianamine synthase 2 | chr5:22711402-22712364 REVERSE LENGTH=320)

HSP 1 Score: 138.658 bits (348), Expect = 2.007e-33
Identity = 78/153 (50.98%), Postives = 99/153 (64.71%), Query Frame = -1

Query:  104 RGQDLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARK---------CSVNQEVYLKSKCCEVEGFINPLNHG---NVIEELSXDE 526
            R  DLSKRM F   ++   K  L  Y+VVFLAALVGMDK+ K K I HL K MA GA++++RSA+G RAFLYPIVD  CDLKGFEVL+I+HP D+V+NSV++ARK           + + V +   C +V   +N  N G   N+IEE S  E
Sbjct:  171 RDSDLSKRMIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVD-SCDLKGFEVLTIYHPSDDVVNSVVIARKLGGSNGARGSQIGRCVVMPCNCSKVHAILN--NRGMEKNLIEEYSAIE 320

[Close]

BLAST of HO066780 vs. TAIR peptide
Match: AT1G56430.1 (| Symbols: ATNAS4, NAS4 | nicotianamine synthase 4 | chr1:21137023-21137997 FORWARD LENGTH=324)

HSP 1 Score: 134.42 bits (337), Expect = 3.785e-32
Identity = 73/145 (50.34%), Postives = 99/145 (68.28%), Query Frame = -1

Query:  113 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKCS----------VNQEVYLKSKCCEVEGFINPLNHGNVIEELS 517
            DLS+RMFF   +I  V   LK ++VVFLAALVGMDKKEK KV+ HL K M+ GA+L++RSA+G RAFLYPIV+  CDL+GFEVLS++HP DEVINS++++RK            ++Q   L   C ++   +N     ++IEE +
Sbjct:  175 DLSQRMFFHTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEP-CDLEGFEVLSVYHPTDEVINSIVISRKLGEDANGVVHDHIDQASDLACNCSKIHVIMN--KKKSIIEEFA 316

[Close]

BLAST of HO066780 vs. TAIR peptide
Match: AT5G04950.1 (| Symbols: NAS1, ATNAS1 | nicotianamine synthase 1 | chr5:1457876-1458838 REVERSE LENGTH=320)

HSP 1 Score: 130.568 bits (327), Expect = 5.466e-31
Identity = 72/150 (48.00%), Postives = 95/150 (63.33%), Query Frame = -1

Query:  104 RGQDLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARKC------SVNQE---VYLKSKCCEVEGFINPLNHGNVIEELSXDE 526
            R  DLSKRM F   ++      L  Y+VVFLAALVGMDK+ K K I HL K MA GA+L++RSA+  RAFLYPIVD   DLKGF++L+I+HP D+V+NSV++ARK        VN     +++   C ++   +N     N+IEE S  E
Sbjct:  172 RDPDLSKRMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD-SSDLKGFQLLTIYHPTDDVVNSVVIARKLGGPTTPGVNGTRGCMFMPCNCSKIHAIMNNRGKKNMIEEFSAIE 320

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BLAST of HO066780 vs. TAIR peptide
Match: AT1G09240.1 (| Symbols: NAS3, ATNAS3 | nicotianamine synthase 3 | chr1:2984950-2985912 FORWARD LENGTH=320)

HSP 1 Score: 130.183 bits (326), Expect = 7.138e-31
Identity = 65/102 (63.73%), Postives = 84/102 (82.35%), Query Frame = -1

Query:  212 DLSKRMFFXXNNIFYVKNVLKDYNVVFLAALVGMDKKEKTKVINHLAKXMADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHPIDEVINSVIVARK 517
            D+S+RMFF   +I  V   LK ++VVFLAALVGM+K+EK KVI HL K MA GA+L++RSA+G RAFLYPIV+  CDL+GFEVLSI+HP D+VINSV++++K
Sbjct:  174 DISQRMFFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVEP-CDLQGFEVLSIYHPTDDVINSVVISKK 274

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BLAST of HO066780 vs. TAIR peptide
Match: AT4G26483.1 (| Symbols: | nicotianamine synthases | chr4:13376647-13376970 REVERSE LENGTH=84)

HSP 1 Score: 60.4622 bits (145), Expect = 6.946e-10
Identity = 34/55 (61.82%), Postives = 41/55 (74.55%), Query Frame = -1

Query:  212 MADGAILVVRSAYGARAFLYPIVDHCCDLKGFEVLSIFHP--IDEVINSVIVARK 370
            M  GA+L++RSA G R+FLY  VD  CDLKGFEVL I+HP   D  +NSV+VARK
Sbjct:    1 MDPGALLMLRSARGLRSFLYVDVDP-CDLKGFEVLEIYHPSMSDGFVNSVMVARK 54

The following BLAST results are available for this feature:

BLAST of HO066780 vs. SwissProt
BLAST of HO066780 vs. TrEMBL
BLAST of HO066780 vs. TAIR peptide

BLAST of HO066780 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NAS_SOLLC	1.166e-34	54.05	Nicotianamine synthase OS=Solanum lycopersicum GN=...	[more]
NAS2_ARATH	1.861e-32	50.98	Nicotianamine synthase 2 OS=Arabidopsis thaliana G...	[more]
NAS4_ARATH	3.510e-31	50.34	Probable nicotianamine synthase 4 OS=Arabidopsis t...	[more]
NAS1_ARATH	5.069e-30	48.00	Nicotianamine synthase 1 OS=Arabidopsis thaliana G...	[more]
NAS3_ARATH	6.620e-30	63.73	Nicotianamine synthase 3 OS=Arabidopsis thaliana G...	[more]
NAS3_ORYSJ	1.209e-23	56.73	Nicotianamine synthase 3 OS=Oryza sativa subsp. ja...	[more]
NAS3_ORYSI	1.209e-23	56.73	Nicotianamine synthase 3 OS=Oryza sativa subsp. in...	[more]
NAS2_ORYSJ	1.209e-23	49.66	Nicotianamine synthase 2 OS=Oryza sativa subsp. ja...	[more]
NAS9_HORVU	4.595e-23	53.45	Nicotianamine synthase 9 OS=Hordeum vulgare GN=NAS...	[more]
NAS1_ORYSJ	1.024e-22	57.39	Nicotianamine synthase 1 OS=Oryza sativa subsp. ja...	[more]

BLAST of HO066780 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TI45_SOYBN	3.376e-44	70.71	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9A9Z2_LOTJA	3.376e-44	72.99	Nicotianamine synthase OS=Lotus japonicus GN=LjNAS...	[more]
B9RFJ1_RICCO	3.160e-42	61.33	Nicotianamine synthase, putative OS=Ricinus commun...	[more]
A3DUI9_MALXI	1.734e-40	57.14	Nicotianamine synthase OS=Malus xiaojinensis PE=2 ...	[more]
C6TI94_SOYBN	1.468e-39	56.95	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9N4R2_POPTR	4.271e-39	57.62	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A2Q2X7_MEDTR	1.623e-38	75.68	Nicotianamine synthase OS=Medicago truncatula GN=M...	[more]
B9RZP0_RICCO	6.818e-37	59.18	Nicotianamine synthase, putative OS=Ricinus commun...	[more]
A5B0Y3_VITVI	8.335e-35	57.53	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7UA30_VITVI	1.422e-34	56.08	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of HO066780 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT5G56080.1	2.007e-33	50.98	\| Symbols: ATNAS2, NAS2 \| nicotianamine synthase 2...	[more]
AT1G56430.1	3.785e-32	50.34	\| Symbols: ATNAS4, NAS4 \| nicotianamine synthase 4...	[more]
AT5G04950.1	5.466e-31	48.00	\| Symbols: NAS1, ATNAS1 \| nicotianamine synthase 1...	[more]
AT1G09240.1	7.138e-31	63.73	\| Symbols: NAS3, ATNAS3 \| nicotianamine synthase 3...	[more]
AT4G26483.1	6.946e-10	61.82	\| Symbols: \| nicotianamine synthases \| chr4:13376...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 9..21 score
None	No IPR available	SEG	seg	seg	coord: 42..62 score
None	No IPR available	PFAM	PF03059	NAS	coord: 11..116 score: 3.8
IPR004298	Nicotianamine synthase	PROFILE	PS51142	NAS	coord: 1..118 score: 19

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO066780 ID=HO066780; Name=HO066780; organism=Cicer arietinum; type=EST; length=550bp
AAAAAAAACATTGAATTCATTACGGCAATGNAATCTCATATCACAAATAA
ACATTCTCAACAACAAATTACATGCATTATTAAGAATAAATACATTAAGC
TTTCTCATCAANGCTCAACTCCTCGATAACATTCCCATGATTCAAAGGAT
TAATAAAACCTTCAACTTCACAACACTTGCTTTTCAAGTAAACCTCTTGA
TTCACCGAACACTTCCTTGCAACAATAACCGAATTTATAACCTCATCAAT
TGGATGAAAAATTGAAAGAACTTCAAAACCTTTAAGATCACAACAATGAT
CAACAATAGGGTATAGAAAAGCTCTAGCACCATAAGCACTTCTCACAACA
AGAATAGCACCATCAGCCATGNATTTGGCCAAATGATTAATCACTTTTGT
TTTTTCCTTTTTATCCATGCCAACCAATGCAGCCAAGAACACAACATTAT
AATCTTTAAGAACATTTTTCACATAAAAAATGTTATTAGNGNGAAAAAAC
ATTCTTTTTGATAAGTCTTGCCCGCGTACCTGCCCGGGCGGCCGCTCGAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0030410	nicotianamine synthase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0030418	nicotianamine biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR004298	Nicotian_synth

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