CK148661

EST Overview


Name	CK148661
Unique Name	CK148661
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	463

Libraries


Library Name	Type
LIBEST_014674 Suppressive subtractive hybridization library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of CK148661 vs. SwissProt
Match: E138_ARATH (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana GN=At1g64760 PE=1 SV=2)

HSP 1 Score: 154.836 bits (390), Expect = 1.636e-37
Identity = 79/122 (64.75%), Postives = 91/122 (74.59%), Query Frame = -2

Query:   94 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAKD 459
            G G   I+VG VG    +GDK ANAG+A RF NG +      K    RP +IEVYLFGL+DEDAKSIAPG FERHWGIF++DGQPKFP+DL+GQGQ+KFLIGAQNV YL   WC  NP+AKD
Sbjct:  255 GHGDMPIIVGEVGWPT-EGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKD 375

HSP 2 Score: 55.8398 bits (133), Expect = 1.012e-7
Identity = 25/40 (62.50%), Postives = 30/40 (75.00%), Query Frame = -3

Query:    3 WLTLMP--KMLSKLADNLNFACAYGDCTALGYGSSCNNLD 116
            W T  P  K L+KLA N+++AC + DCTALGYGSSCN LD
Sbjct:  366 WCTFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLD 405

[Close]

BLAST of CK148661 vs. SwissProt
Match: E135_ARATH (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana GN=At4g31140 PE=1 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 3.955e-30
Identity = 60/120 (50.00%), Postives = 75/120 (62.50%), Query Frame = -2

Query:  100 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDA 459
            G G   I+VG VG     GDK AN   A R+  G ++  +  K    RPG ++ YLFGLIDEDAKSI PGNFERHWGIF  DGQPK+ + L   G    LI A++V YL+  WC++ P+A
Sbjct:  257 GFGNLTIIVGEVGWPT-DGDKNANLMYARRYNQGFMNRQKANKGTPMRPGAMDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSL---GSGNGLIPAKDVHYLAKKWCILAPNA 372

HSP 2 Score: 41.2022 bits (95), Expect = 3.955e-30
Identity = 15/28 (53.57%), Postives = 23/28 (82.14%), Query Frame = -3

Query:    3 KLADNLNFACAYGDCTALGYGSSCNNLD 86
            +L  ++++AC + DCT+LGYGSSC NL+
Sbjct:  378 QLGPSVSYACDHADCTSLGYGSSCGNLN 405

[Close]

BLAST of CK148661 vs. SwissProt
Match: E136_ARATH (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana GN=At5g58090 PE=1 SV=2)

HSP 1 Score: 100.908 bits (250), Expect = 3.766e-27
Identity = 53/121 (43.80%), Postives = 71/121 (58.68%), Query Frame = -2

Query:   97 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAK 459
            G G   I++G +G     GD  AN   A +F  G ++     K    RPG I+ YLF LIDEDAKS+ PG FERHWGIF +DG PK+ ++L G      LI A+ V+YL   WC++ P+ +
Sbjct:  252 GFGNMPIIIGEIGWPT-DGDSNANLDYAKKFNQGFMAHISGGKGTPRRPGPIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLERKWCVMKPNVR 370

HSP 2 Score: 40.4318 bits (93), Expect = 3.766e-27
Identity = 17/41 (41.46%), Postives = 27/41 (65.85%), Query Frame = -3

Query:    3 WLTLMPKML---SKLADNLNFACAYGDCTALGYGSSCNNLD 116
            W  + P +     ++A  +++AC+ GDCT+LG G+SC NLD
Sbjct:  362 WCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLD 402

[Close]

BLAST of CK148661 vs. SwissProt
Match: E139_ARATH (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana GN=At5g58480 PE=1 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.213e-16
Identity = 44/117 (37.61%), Postives = 64/117 (54.70%), Query Frame = -2

Query:  109 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVN 459
            G     I+V  +G     G + A +  A  F  G I   ++    L RP  +E Y+  L+DED ++++ GNFERHWG+F +DGQ K+      + Q    + AQNV+YL P WC+VN
Sbjct:  257 GFSEVDIVVSKIGWPT-DGAENATSLTAEAFFKGLIVHLEKKTASLPRPP-VETYIESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFNHKNQ----VNAQNVQYLPPKWCVVN 367

HSP 2 Score: 25.7942 bits (55), Expect = 1.213e-16
Identity = 12/31 (38.71%), Postives = 17/31 (54.84%), Query Frame = -3

Query:    6 KMLSKLADNLNFACAYGDCTALGYGSSCNNL 98
            K LS  +     ACA  DCT++  G SC+ +
Sbjct:  370 KDLSNASARALEACAVADCTSILPGGSCSGI 400

[Close]

BLAST of CK148661 vs. TrEMBL
Match: A5BTD4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041708 PE=3 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 3.665e-46
Identity = 77/125 (61.60%), Postives = 92/125 (73.60%), Query Frame = -2

Query:   94 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLG---RPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAKD 459
            G+G   ILVG VG     GDK AN+ NA RF NG   +  +   ++G   RPG+IEVYLFGLIDEDAKSIAPGNFERHWGIF+YDGQPK+ +DL+GQGQNK L+ A++V YL   WCM NP+A D
Sbjct:  256 GLGDMTILVGEVGWPT-DGDKNANSNNAYRFYNG---LLPRLAGNIGTPLRPGYIEVYLFGLIDEDAKSIAPGNFERHWGIFKYDGQPKYALDLSGQGQNKLLVAAKDVTYLPQKWCMFNPNAGD 376

HSP 2 Score: 56.9954 bits (136), Expect = 3.665e-46
Identity = 23/30 (76.67%), Postives = 26/30 (86.67%), Query Frame = -3

Query:    3 LSKLADNLNFACAYGDCTALGYGSSCNNLD 92
            LSKL DN+N+AC + DCTALGYGSSCN LD
Sbjct:  377 LSKLGDNINYACTFSDCTALGYGSSCNGLD 406

[Close]

BLAST of CK148661 vs. TrEMBL
Match: Q9ZUP5_ARATH (Putative beta-1,3-glucanase OS=Arabidopsis thaliana GN=At2g19440 PE=2 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.947e-44
Identity = 75/122 (61.48%), Postives = 90/122 (73.77%), Query Frame = -2

Query:   94 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAKD 459
            G G   I+VG VG    +GDK AN+G+A RF NG +    + +    RP +IEVYLFGL+DEDAKSIAPG FERHWGIF++DGQPKFP+DL+GQGQNK LIGA+NV Y    WCM N +AKD
Sbjct:  251 GHGDMPIIVGEVGWPT-EGDKHANSGSAYRFYNGLLPRLGENRGTPLRPTYIEVYLFGLLDEDAKSIAPGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAKD 371

HSP 2 Score: 53.1434 bits (126), Expect = 1.947e-44
Identity = 22/32 (68.75%), Postives = 27/32 (84.38%), Query Frame = -3

Query:    3 KMLSKLADNLNFACAYGDCTALGYGSSCNNLD 98
            K L+KLA N+++AC + DCTALGYGSSCN LD
Sbjct:  370 KDLTKLAANIDYACTFSDCTALGYGSSCNTLD 401

[Close]

BLAST of CK148661 vs. TrEMBL
Match: D7LXS8_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_661672 PE=4 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 1.243e-43
Identity = 74/119 (62.18%), Postives = 84/119 (70.59%), Query Frame = -2

Query:  103 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPD 459
            G G  +++VG VG     GDK AN  NA RF  G +      +    RPG+IEVYLFG IDEDAKSIAPGNFERHWGIF+YDGQPKFP DL+G GQ K L GAQNV+YL   WCM+NPD
Sbjct:  260 GHGDMSVIVGEVGWPT-DGDKNANIPNAERFYTGLLPKLAANRGTPMRPGYIEVYLFGFIDEDAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYLQNQWCMLNPD 377

HSP 2 Score: 52.7582 bits (125), Expect = 1.243e-43
Identity = 24/44 (54.55%), Postives = 29/44 (65.91%), Query Frame = -3

Query:    3 WLTLMPKMLS------KLADNLNFACAYGDCTALGYGSSCNNLD 116
            W  L P  L+      +L DN+N+AC + DCTALGYGSSC NLD
Sbjct:  371 WCMLNPDGLTFSNNTNQLGDNVNYACTFSDCTALGYGSSCGNLD 414

[Close]

BLAST of CK148661 vs. TrEMBL
Match: A2Q5Q4_MEDTR (Glycoside hydrolase, family 17; X8 OS=Medicago truncatula GN=MtrDRAFT_AC167711g16v2 PE=3 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 2.162e-43
Identity = 89/122 (72.95%), Postives = 96/122 (78.69%), Query Frame = -2

Query:   94 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAKD 459
            G G T ILVG VG    +GDK AN+GNA+RF NG +S     +    RPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDL+GQ QNK L+GAQ VKYL P WCM NPDAKD
Sbjct:  257 GFGDTPILVGEVGWPT-EGDKNANSGNAIRFYNGLLSRLAANRGTPLRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLSGQLQNKLLVGAQGVKYLDPMWCMFNPDAKD 377

HSP 2 Score: 65.4698 bits (158), Expect = 1.990e-9
Identity = 30/40 (75.00%), Postives = 32/40 (80.00%), Query Frame = -3

Query:    3 WLTLMP--KMLSKLADNLNFACAYGDCTALGYGSSCNNLD 116
            W    P  K LSKLADN+NFAC +GDCTALGYGSSCNNLD
Sbjct:  368 WCMFNPDAKDLSKLADNINFACTFGDCTALGYGSSCNNLD 407

[Close]

BLAST of CK148661 vs. TrEMBL
Match: Q9FK49_ARATH (Beta-1,3-glucanase-like protein OS=Arabidopsis thaliana GN=At5g18220 PE=3 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 6.086e-43
Identity = 74/120 (61.67%), Postives = 83/120 (69.17%), Query Frame = -2

Query:  100 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDA 459
            G G   ++VG VG     GDK AN  NA RF  G +      +    RPG+IEVYLFG IDEDAKSIAPGNFERHWGIF+YDGQPKFP DL+G GQ K L GAQNV+YL   WCM NP+A
Sbjct:  258 GHGDMTVIVGEVGWPT-DGDKNANIPNAERFYTGLLPKLAANRGTPMRPGYIEVYLFGFIDEDAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYLRNQWCMFNPNA 376

HSP 2 Score: 52.373 bits (124), Expect = 6.086e-43
Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = -3

Query:    3 SKLADNLNFACAYGDCTALGYGSSCNNLD 89
            ++L DN+N+AC + DCTALGYGSSC NLD
Sbjct:  384 NQLGDNVNYACTFSDCTALGYGSSCGNLD 412

[Close]

BLAST of CK148661 vs. TrEMBL
Match: Q9SQR1_ARATH (At3g04010 OS=Arabidopsis thaliana GN=At3g04010 PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 3.883e-42
Identity = 70/122 (57.38%), Postives = 85/122 (69.67%), Query Frame = -2

Query:   97 GYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRP---GFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAK 453
            G   ++VG VG     GDK AN  NA RF +G   +  +   ++G P   G+IEVYLFG IDEDAKS+APGNFERHWGIF++DGQPKFP+D  GQGQ KFL GAQNV+Y    WCM NP+ +
Sbjct:  265 GNMEVIVGEVGWPT-DGDKNANVPNAERFYSG---LLPRLANNVGTPMRKGYIEVYLFGFIDEDAKSVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYFLNQWCMFNPNGR 382

HSP 2 Score: 55.0694 bits (131), Expect = 3.883e-42
Identity = 21/30 (70.00%), Postives = 27/30 (90.00%), Query Frame = -3

Query:    3 LSKLADNLNFACAYGDCTALGYGSSCNNLD 92
            +S+L DN+N+AC++ DCTALGYGSSC NLD
Sbjct:  385 MSRLGDNINYACSHSDCTALGYGSSCGNLD 414

[Close]

BLAST of CK148661 vs. TrEMBL
Match: D7L207_ARALY (Glycosyl hydrolase family 17 protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_340560 PE=4 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 6.593e-42
Identity = 70/122 (57.38%), Postives = 85/122 (69.67%), Query Frame = -2

Query:   97 GYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRP---GFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAK 453
            G   ++VG VG     GDK AN  NA RF +G   +  +   ++G P   G+IEVYLFG IDED KS+APGNFERHWGIF++DGQPKFP+D  GQGQ KFL GAQNV+YL   WCM NP+ +
Sbjct:  265 GNMEVIVGEVGWPT-DGDKNANVQNAERFYSG---LLPRLANNVGTPMRKGYIEVYLFGFIDEDVKSVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYLLNQWCMFNPNGR 382

HSP 2 Score: 54.299 bits (129), Expect = 6.593e-42
Identity = 21/30 (70.00%), Postives = 26/30 (86.67%), Query Frame = -3

Query:    3 LSKLADNLNFACAYGDCTALGYGSSCNNLD 92
            +S L DN+N+AC++ DCTALGYGSSC NLD
Sbjct:  385 MSSLGDNINYACSHSDCTALGYGSSCGNLD 414

[Close]

BLAST of CK148661 vs. TrEMBL
Match: Q852K4_ORYSJ (Glucan endo-1,3-beta-glucosidase 5, putative, expressed OS=Oryza sativa subsp. japonica GN=OSJNBb0060J21.19 PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.058e-40
Identity = 71/123 (57.72%), Postives = 85/123 (69.11%), Query Frame = -2

Query:   94 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPG-FIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAKD 459
            G G   I+VG VG     GDK A A  A RF NG +           RPG +IEVYLFGL+DEDAKS+APG+FERHWGI R+DGQPK+P+DLTGQGQN  L+ A+ V YL  TWC++N +AKD
Sbjct:  265 GHGDMPIVVGEVGWPT-DGDKHATATYAQRFYNGLLKRLAANAGTPARPGQYIEVYLFGLLDEDAKSVAPGDFERHWGILRFDGQPKYPVDLTGQGQNTMLVPAKGVTYLPRTWCVINTNAKD 386

HSP 2 Score: 53.5286 bits (127), Expect = 2.058e-40
Identity = 22/32 (68.75%), Postives = 26/32 (81.25%), Query Frame = -3

Query:    3 KMLSKLADNLNFACAYGDCTALGYGSSCNNLD 98
            K  SKLADN+NFAC + DCTALGYGS+C  +D
Sbjct:  385 KDTSKLADNINFACTFADCTALGYGSTCAGMD 416

[Close]

BLAST of CK148661 vs. TrEMBL
Match: A2XMT6_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_13858 PE=3 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.058e-40
Identity = 71/123 (57.72%), Postives = 85/123 (69.11%), Query Frame = -2

Query:   94 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPG-FIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAKD 459
            G G   I+VG VG     GDK A A  A RF NG +           RPG +IEVYLFGL+DEDAKS+APG+FERHWGI R+DGQPK+P+DLTGQGQN  L+ A+ V YL  TWC++N +AKD
Sbjct:  265 GHGDMPIVVGEVGWPT-DGDKHATATYAQRFYNGLLKRLAANAGTPARPGQYIEVYLFGLLDEDAKSVAPGDFERHWGILRFDGQPKYPVDLTGQGQNTMLVPAKGVTYLPRTWCVINTNAKD 386

HSP 2 Score: 53.5286 bits (127), Expect = 2.058e-40
Identity = 22/32 (68.75%), Postives = 26/32 (81.25%), Query Frame = -3

Query:    3 KMLSKLADNLNFACAYGDCTALGYGSSCNNLD 98
            K  SKLADN+NFAC + DCTALGYGS+C  +D
Sbjct:  385 KDTSKLADNINFACTFADCTALGYGSTCAGMD 416

[Close]

BLAST of CK148661 vs. TrEMBL
Match: Q0DMU8_ORYSJ (Os03g0792800 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os03g0792800 PE=3 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.063e-40
Identity = 71/123 (57.72%), Postives = 85/123 (69.11%), Query Frame = -2

Query:   94 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPG-FIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAKD 459
            G G   I+VG VG     GDK A A  A RF NG +           RPG +IEVYLFGL+DEDAKS+APG+FERHWGI R+DGQPK+P+DLTGQGQN  L+ A+ V YL  TWC++N +AKD
Sbjct:  173 GHGDMPIVVGEVGWPT-DGDKHATATYAQRFYNGLLKRLAANAGTPARPGQYIEVYLFGLLDEDAKSVAPGDFERHWGILRFDGQPKYPVDLTGQGQNTMLVPAKGVTYLPRTWCVINTNAKD 294

HSP 2 Score: 53.5286 bits (127), Expect = 2.063e-40
Identity = 22/32 (68.75%), Postives = 26/32 (81.25%), Query Frame = -3

Query:    3 KMLSKLADNLNFACAYGDCTALGYGSSCNNLD 98
            K  SKLADN+NFAC + DCTALGYGS+C  +D
Sbjct:  293 KDTSKLADNINFACTFADCTALGYGSTCAGMD 324

[Close]

BLAST of CK148661 vs. TAIR peptide
Match: AT2G19440.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr2:8418164-8419806 REVERSE LENGTH=478)

HSP 1 Score: 150.214 bits (378), Expect = 1.437e-46
Identity = 75/122 (61.48%), Postives = 90/122 (73.77%), Query Frame = -2

Query:   94 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAKD 459
            G G   I+VG VG    +GDK AN+G+A RF NG +    + +    RP +IEVYLFGL+DEDAKSIAPG FERHWGIF++DGQPKFP+DL+GQGQNK LIGA+NV Y    WCM N +AKD
Sbjct:  251 GHGDMPIIVGEVGWPT-EGDKHANSGSAYRFYNGLLPRLGENRGTPLRPTYIEVYLFGLLDEDAKSIAPGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAKD 371

HSP 2 Score: 53.1434 bits (126), Expect = 1.437e-46
Identity = 22/32 (68.75%), Postives = 27/32 (84.38%), Query Frame = -3

Query:    3 KMLSKLADNLNFACAYGDCTALGYGSSCNNLD 98
            K L+KLA N+++AC + DCTALGYGSSCN LD
Sbjct:  370 KDLTKLAANIDYACTFSDCTALGYGSSCNTLD 401

[Close]

BLAST of CK148661 vs. TAIR peptide
Match: AT5G18220.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr5:6018914-6020453 REVERSE LENGTH=488)

HSP 1 Score: 145.976 bits (367), Expect = 4.490e-45
Identity = 74/120 (61.67%), Postives = 83/120 (69.17%), Query Frame = -2

Query:  100 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDA 459
            G G   ++VG VG     GDK AN  NA RF  G +      +    RPG+IEVYLFG IDEDAKSIAPGNFERHWGIF+YDGQPKFP DL+G GQ K L GAQNV+YL   WCM NP+A
Sbjct:  258 GHGDMTVIVGEVGWPT-DGDKNANIPNAERFYTGLLPKLAANRGTPMRPGYIEVYLFGFIDEDAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYLRNQWCMFNPNA 376

HSP 2 Score: 52.373 bits (124), Expect = 4.490e-45
Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = -3

Query:    3 SKLADNLNFACAYGDCTALGYGSSCNNLD 89
            ++L DN+N+AC + DCTALGYGSSC NLD
Sbjct:  384 NQLGDNVNYACTFSDCTALGYGSSCGNLD 412

[Close]

BLAST of CK148661 vs. TAIR peptide
Match: AT3G04010.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr3:1036879-1039263 REVERSE LENGTH=491)

HSP 1 Score: 140.584 bits (353), Expect = 2.865e-44
Identity = 70/122 (57.38%), Postives = 85/122 (69.67%), Query Frame = -2

Query:   97 GYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRP---GFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAK 453
            G   ++VG VG     GDK AN  NA RF +G   +  +   ++G P   G+IEVYLFG IDEDAKS+APGNFERHWGIF++DGQPKFP+D  GQGQ KFL GAQNV+Y    WCM NP+ +
Sbjct:  265 GNMEVIVGEVGWPT-DGDKNANVPNAERFYSG---LLPRLANNVGTPMRKGYIEVYLFGFIDEDAKSVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYFLNQWCMFNPNGR 382

HSP 2 Score: 55.0694 bits (131), Expect = 2.865e-44
Identity = 21/30 (70.00%), Postives = 27/30 (90.00%), Query Frame = -3

Query:    3 LSKLADNLNFACAYGDCTALGYGSSCNNLD 92
            +S+L DN+N+AC++ DCTALGYGSSC NLD
Sbjct:  385 MSRLGDNINYACSHSDCTALGYGSSCGNLD 414

[Close]

BLAST of CK148661 vs. TAIR peptide
Match: AT1G64760.2 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr1:24054220-24056194 REVERSE LENGTH=481)

HSP 1 Score: 154.836 bits (390), Expect = 1.897e-38
Identity = 79/122 (64.75%), Postives = 91/122 (74.59%), Query Frame = -2

Query:   94 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAKD 459
            G G   I+VG VG    +GDK ANAG+A RF NG +      K    RP +IEVYLFGL+DEDAKSIAPG FERHWGIF++DGQPKFP+DL+GQGQ+KFLIGAQNV YL   WC  NP+AKD
Sbjct:  255 GHGDMPIIVGEVGWPT-EGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKD 375

HSP 2 Score: 55.8398 bits (133), Expect = 1.180e-8
Identity = 25/40 (62.50%), Postives = 30/40 (75.00%), Query Frame = -3

Query:    3 WLTLMP--KMLSKLADNLNFACAYGDCTALGYGSSCNNLD 116
            W T  P  K L+KLA N+++AC + DCTALGYGSSCN LD
Sbjct:  366 WCTFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLD 405

[Close]

BLAST of CK148661 vs. TAIR peptide
Match: AT1G64760.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr1:24054220-24056194 REVERSE LENGTH=481)

HSP 1 Score: 154.836 bits (390), Expect = 1.897e-38
Identity = 79/122 (64.75%), Postives = 91/122 (74.59%), Query Frame = -2

Query:   94 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAKD 459
            G G   I+VG VG    +GDK ANAG+A RF NG +      K    RP +IEVYLFGL+DEDAKSIAPG FERHWGIF++DGQPKFP+DL+GQGQ+KFLIGAQNV YL   WC  NP+AKD
Sbjct:  255 GHGDMPIIVGEVGWPT-EGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKD 375

HSP 2 Score: 55.8398 bits (133), Expect = 1.180e-8
Identity = 25/40 (62.50%), Postives = 30/40 (75.00%), Query Frame = -3

Query:    3 WLTLMP--KMLSKLADNLNFACAYGDCTALGYGSSCNNLD 116
            W T  P  K L+KLA N+++AC + DCTALGYGSSCN LD
Sbjct:  366 WCTFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLD 405

[Close]

BLAST of CK148661 vs. TAIR peptide
Match: AT3G24330.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr3:8830336-8831838 FORWARD LENGTH=500)

HSP 1 Score: 120.553 bits (301), Expect = 1.857e-36
Identity = 59/115 (51.30%), Postives = 79/115 (68.70%), Query Frame = -2

Query:  100 ILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFI-EVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDA 441
            I+VG VG     GDK AN  +A RF  G +  +        R G I +VYLF L+DEDAKSIAPG FERHWGIF +DG+PK+ +DL+G+G +K L+  ++VKYL  TWC+++P+A
Sbjct:  276 IIVGEVGWPT-DGDKNANVKSAKRFNQGMVKHAMSGNGTPARKGVIMDVYLFSLVDEDAKSIAPGTFERHWGIFEFDGRPKYELDLSGKGNDKPLVPVEDVKYLPKTWCILDPNA 389

HSP 2 Score: 48.9062 bits (115), Expect = 1.857e-36
Identity = 22/39 (56.41%), Postives = 27/39 (69.23%), Query Frame = -3

Query:    6 WLTLMPKM--LSKLADNLNFACAYGDCTALGYGSSCNNL 116
            W  L P    L  L DN+++AC+  DCTALGYGSSCN+L
Sbjct:  382 WCILDPNAYNLDDLPDNIDYACSLSDCTALGYGSSCNHL 420

[Close]

BLAST of CK148661 vs. TAIR peptide
Match: AT4G31140.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr4:15141581-15143188 FORWARD LENGTH=484)

HSP 1 Score: 110.153 bits (274), Expect = 4.570e-31
Identity = 60/120 (50.00%), Postives = 75/120 (62.50%), Query Frame = -2

Query:  100 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDA 459
            G G   I+VG VG     GDK AN   A R+  G ++  +  K    RPG ++ YLFGLIDEDAKSI PGNFERHWGIF  DGQPK+ + L   G    LI A++V YL+  WC++ P+A
Sbjct:  257 GFGNLTIIVGEVGWPT-DGDKNANLMYARRYNQGFMNRQKANKGTPMRPGAMDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSL---GSGNGLIPAKDVHYLAKKWCILAPNA 372

HSP 2 Score: 41.2022 bits (95), Expect = 4.570e-31
Identity = 15/28 (53.57%), Postives = 23/28 (82.14%), Query Frame = -3

Query:    3 KLADNLNFACAYGDCTALGYGSSCNNLD 86
            +L  ++++AC + DCT+LGYGSSC NL+
Sbjct:  378 QLGPSVSYACDHADCTSLGYGSSCGNLN 405

[Close]

BLAST of CK148661 vs. TAIR peptide
Match: AT5G64790.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr5:25902713-25904559 FORWARD LENGTH=485)

HSP 1 Score: 105.916 bits (263), Expect = 4.349e-28
Identity = 58/119 (48.74%), Postives = 71/119 (59.66%), Query Frame = -2

Query:  103 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPD 459
            GI    I+VG VG     G K A+   A +F  G +    +      RP  +EVYLFG +DED KSI PG FERHWGIFRYDG PKF +D TGQG+    + A+ V+YL   WC+VN D
Sbjct:  258 GIHDMKIIVGEVGWPT-DGHKYASPKLAEKFYAGLMKRLAKDGGTPTRPERLEVYLFGFLDEDMKSILPGPFERHWGIFRYDGTPKFMLDFTGQGRQMVPVAAKGVQYLEKQWCVVNKD 375

HSP 2 Score: 35.4242 bits (80), Expect = 4.349e-28
Identity = 13/29 (44.83%), Postives = 22/29 (75.86%), Query Frame = -3

Query:    6 LSKLADNLNFACAYGDCTALGYGSSCNNL 92
            L ++  +L++AC +GDCTA+  GS+C+ L
Sbjct:  379 LDEVGPDLDYACYHGDCTAMEAGSTCSKL 407

[Close]

BLAST of CK148661 vs. TAIR peptide
Match: AT5G58090.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr5:23505556-23507193 REVERSE LENGTH=477)

HSP 1 Score: 100.908 bits (250), Expect = 4.350e-28
Identity = 53/121 (43.80%), Postives = 71/121 (58.68%), Query Frame = -2

Query:   97 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNPDAK 459
            G G   I++G +G     GD  AN   A +F  G ++     K    RPG I+ YLF LIDEDAKS+ PG FERHWGIF +DG PK+ ++L G      LI A+ V+YL   WC++ P+ +
Sbjct:  252 GFGNMPIIIGEIGWPT-DGDSNANLDYAKKFNQGFMAHISGGKGTPRRPGPIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLERKWCVMKPNVR 370

HSP 2 Score: 40.4318 bits (93), Expect = 4.350e-28
Identity = 17/41 (41.46%), Postives = 27/41 (65.85%), Query Frame = -3

Query:    3 WLTLMPKML---SKLADNLNFACAYGDCTALGYGSSCNNLD 116
            W  + P +     ++A  +++AC+ GDCT+LG G+SC NLD
Sbjct:  362 WCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLD 402

[Close]

BLAST of CK148661 vs. TAIR peptide
Match: AT4G17180.1 (| Symbols: | O-Glycosyl hydrolases family 17 protein | chr4:9646541-9648045 FORWARD LENGTH=475)

HSP 1 Score: 93.2041 bits (230), Expect = 1.258e-23
Identity = 57/120 (47.50%), Postives = 71/120 (59.17%), Query Frame = -2

Query:  106 GIGYTAILVGGVGMRLRKGDKGANAGNALRFTNGPISISQQAKEHLGRPGF--IEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLTGQGQNKFLIGAQNVKYLSPTWCMVNP 459
            G G   I++G +G     G  GAN   A  F  G IS     K    RPG    +VYLFGL+DE AKS  PGNFERHWGIF +DGQ K+ ++L G G N+ L  A+NV+YL   WC+ +P
Sbjct:  248 GYGQMPIVIGEIGWPT-DGAVGANLTAARVFNQGLISHVLSNKGTPLRPGSPPADVYLFGLLDEGAKSTLPGNFERHWGIFSFDGQAKYRLNL-GLG-NRGLKNAKNVQYLPSRWCVAHP 364

HSP 2 Score: 33.113 bits (74), Expect = 1.258e-23
Identity = 14/38 (36.84%), Postives = 21/38 (55.26%), Query Frame = -3

Query:    6 WLTLMPKM-LSKLADNLNFACAYGDCTALGYGSSCNNL 116
            W    P   ++++ D+L  AC+  DCT L  G SC+ L
Sbjct:  359 WCVAHPSRDMTQVGDHLRLACSEADCTTLNDGGSCSQL 396

The following BLAST results are available for this feature:

BLAST of CK148661 vs. SwissProt
BLAST of CK148661 vs. TrEMBL
BLAST of CK148661 vs. TAIR peptide

BLAST of CK148661 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
E138_ARATH	1.636e-37	64.75	Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis ...	[more]
E135_ARATH	3.955e-30	50.00	Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis ...	[more]
E136_ARATH	3.766e-27	43.80	Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis ...	[more]
E139_ARATH	1.213e-16	37.61	Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis ...	[more]

BLAST of CK148661 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A5BTD4_VITVI	3.665e-46	61.60	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q9ZUP5_ARATH	1.947e-44	61.48	Putative beta-1,3-glucanase OS=Arabidopsis thalian...	[more]
D7LXS8_ARALY	1.243e-43	62.18	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
A2Q5Q4_MEDTR	2.162e-43	72.95	Glycoside hydrolase, family 17; X8 OS=Medicago tru...	[more]
Q9FK49_ARATH	6.086e-43	61.67	Beta-1,3-glucanase-like protein OS=Arabidopsis tha...	[more]
Q9SQR1_ARATH	3.883e-42	57.38	At3g04010 OS=Arabidopsis thaliana GN=At3g04010 PE=...	[more]
D7L207_ARALY	6.593e-42	57.38	Glycosyl hydrolase family 17 protein OS=Arabidopsi...	[more]
Q852K4_ORYSJ	2.058e-40	57.72	Glucan endo-1,3-beta-glucosidase 5, putative, expr...	[more]
A2XMT6_ORYSI	2.058e-40	57.72	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q0DMU8_ORYSJ	2.063e-40	57.72	Os03g0792800 protein (Fragment) OS=Oryza sativa su...	[more]

BLAST of CK148661 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT2G19440.1	1.437e-46	61.48	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]
AT5G18220.1	4.490e-45	61.67	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]
AT3G04010.1	2.865e-44	57.38	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]
AT1G64760.2	1.897e-38	64.75	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]
AT1G64760.1	1.897e-38	64.75	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]
AT3G24330.1	1.857e-36	51.30	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]
AT4G31140.1	4.570e-31	50.00	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]
AT5G64790.1	4.349e-28	48.74	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]
AT5G58090.1	4.350e-28	43.80	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]
AT4G17180.1	1.258e-23	47.50	\| Symbols: \| O-Glycosyl hydrolases family 17 prot...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 24..34 score
IPR000490	Glycoside hydrolase, family 17	PFAM	PF00332	Glyco_hydro_17	coord: 19..91 score: 2
IPR013781	Glycoside hydrolase, subgroup, catalytic core	GENE3D	3.20.20.80	no description	coord: 7..91 score: 2.5
IPR017853	Glycoside hydrolase, catalytic core	SUPERFAMILY	51445	(Trans)glycosidases	coord: 19..92 score: 4.6

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK148661 ID=CK148661; Name=CK148661; organism=Cicer arietinum; type=EST; length=463bp
GCATCAAGGTTGTTGCAAGAAGATCCATAACCAAGTGCAGTGCAATCTCC
ATAAGCGCAAGCAAAATTAAGGTTATCAGCTAGTTTGCTAAGCATCTTTG
GCATCAGGGTTAACCATGCACCATGTTGGAGAAAGATACTTCACATTTTG
TGCACCTATTAGAAATTTGTTTTGACCTTGACCAGTAAGATCCATTGGAA
ACTTAGGTTGTCCGTCGTATCGAAATATTCCCCAGTGTCTCTCGAAGTTC
CCCGGCGCAATGCTTTTGGCGTCCTCGTCGATGAGTCCGAACAGATAAAC
TTCGATGAATCCAGGACGGCCCAGGTGTTCCTTTGCTTGCTGCGAGATTG
AAATAGGGCCGTTTGTAAATCTTAATGCATTGCCTGCGTTGGCCCCCTTG
TCCCCCTTCCGTAGGCGCATCCCTACTCCTCCTACCAAAATGGCCGTGTA
CCCTATCCCACCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0043169	cation binding

Vocabulary: **INTERPRO**
Term	Definition
IPR000490	Glyco_hydro_17
IPR013781	Glyco_hydro_catalytic_dom
IPR017853	Glycoside_hydrolase_SF

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